CSPI05G05440 (gene) Cucumber (PI 183967) v1

Overview
NameCSPI05G05440
Typegene
OrganismCucumis sativus var. hardwickii cv. PI 183967 (Cucumber (PI 183967) v1)
DescriptionTubulin beta chain
LocationChr5: 5042407 .. 5045472 (+)
RNA-Seq ExpressionCSPI05G05440
SyntenyCSPI05G05440
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
ACAAGAACTAGCAGCTCTCCTCTTCTTGTCACCTTCCCTTGTCATCCAGCTCACACAACTCCAATTTGGAATTTGACAGGTCTCTCCTCACTCATACATTCCCACATGAAATTATTAATTGAATCTTCAACCATGTCTATGATTCTTCAGCTTCACTGTCCTTTTCCACCATTTTTTTCTTCAAGATAAAGACTAGTAAACTCCTTATATATCCCTCTGCCTCTCTTCCCATTCATCTGTGCAAATTCACAAACCATCTCCCATTTCCTTCTTGTTTATCTCTGTTTTTTTATTTTACACATCTGTTGAACTTCCCATCTGAAAAATGAGAGAAATTCTCCATATTCAAGCAGGACAGTGTGGAAACCAAATTGGAGCTAAGTTTTGGGAAGTTGTTTGTGATGAACATGGGATTGATCCTGCTGGAAATTACACCGGAAGTAGTAGCCTTCAACTCGAGAGGGTTAATGTTTACTACAACGAAGCGAGTGGGGGACGATATGTTCCAAGGGCTGTTTTGGTTGATCTTGAACCAGGAACCATGGACAGCCTCAGGACAGGTCCTTATGGGAATATATTTAGACCTGATAACTTTGTTTTTGGGCAAAATGGGGCTGGAAACAACTGGGCTAAGGGTCATTACACTGAAGGAGCTGAACTGGTTGATTCTGTTCTTGATGTGGTTCGCAAAGAAGCTGAGAATTGTGACTGTTTGCAAGGTATGAGTATGAGATTTTTCTTCCTTGATCTTCATTTCTTGGGCCATTTGAGTAGAAAGATGTTAACAATTGCTCCTTAGAGAATGAAAGAACTCTGTTTGGTGTATTGAGATATTGATATTATTGATATATTGTGAATGCGAATTGATTCTATAACCTAAAGCTGAAAAGTGTGAGGTGTCCTGTTTTGGTTAACTTTAACTTATCAAATTATTAGGAGCTTAATATGGAATCATAAGAGGAAACCCAAGAAATGTTGTTGGAATGTCTTAGATTTATTATTTGACTATTCAAAATGCAGTATATAAACTCTCTAACTCTAGAGATGATTCTGAATTATTCTTTCTGATAACAAACTATTCTCCCTTTGCCATAGATTAGATAACAAATTGTCACTGAACTATGTTAACTTTTTGTTGATTCTCTATTACTTTACATGTTTATGTTCTCTTCATCTGTCAATTCTTTAACATTTCCTTTCAAATTAATAATAATGGTACTTAGAAAGTTGTTCCAAATGAACTCTTATATTTGTGAATACAAGTGTTTAACACAAAGGTTGAATTCAAATCTTAGTTTGAATCTCGTTTGGTTGTGTGAAACAGGGTTTCAAGTTTGCCATTCACTTGGAGGAGGGACTGGATCTGGAATGGGAACTCTATTGATATCAAAGATCAGGGAAGAGTATCCTGACAGAATGATGTTAACTTTCTCTGTCTTCCCCTCACCTAAGGTCTCGGATACAGTGGTTGAGCCCTATAATGCCACTCTCTCTGTGCACCAGTTGGTTGAAAATGCTGATGAATGCATGGTTTTGGATAATGAAGCACTCTATGACATCTGCTTTAGGACCCTCAAGCTCACCAATCCAAGTTGTAAGTCTCTCTCTCAATATCACATTCTTGTCTAAGTTAACTATAGTTTTCGTCCTCTCACCACGGTGTGAGCTTTTAGCATCAAGAACGTGTATAGAAGATCAAATACACTTGGTTTAGATAAGGTTTAGAGTCTTAAGATGATAGTGTCGGGATATAAAATGTTGTTTTAAATGGCTCTGATGTTTTTTTCTCCATAATTAAGATTGATAGTAACTTGTAGGATCTCAAGGTAATTTTCGGACTGACAAGAGAGTATTAAGAATATATGAAAAGTTCATAAGATTAGGTATATCTTCACCAGTTAGATCAAGTCTTAGGACTCACGATTAACTCAATACTTGACATGGGTCGTTCAACTCTTTCGTATCTTGTGTGGTTACACACCAAGAGACCAACTCTTAAATCAGTAAGTTGGTTTCAAGCTGTGGGAAGTCTGGTAGAAAATGTGTTGTGTAGCATTCTAACTGGAGATGTAATGGTTGTTTATTTGGATTTATATGTGTTTCAGTTGGTGATCTCAACCACTTGATTTCTACAACCATGAGTGGAGTTACATGCTGCCTTCGCTTCCCTGGCCAGCTCAACTCCGACCTCCGGAAGCTAGCTGTGAACCTCATCCCTTTCCCGCGCCTCCACTTCTTCATGGTCGGTTTTGCTCCCTTGACTGCTCGTGGCTCACAGCAGTACCGTGCACTCACCATCCCTGAGCTCACCCAACAAATGTGGGATGCCAAAAACATGATGTGTGCTGCTGATCCTCGCCATGGTCGATACCTAACTGCCTCAGCTTTATTCCGTGGCAAGATGAGCACCAAGGAAGTCGATGAACAAATGATAAATGTACAGAACAAGAACTCATCTTATTTTGTTGAGTGGATCCCAAACAATGTTAAATCAAGTGTTTGTGACATCCCTCCAACTGGGTTGTCAATGTCTTCAACATTCATGGGAAACTCGACCTCAATACAAGAGATGTTTCGGCGAGTTTCTGAACAATTCACGGTCATGTTCAGGAGGAAGGCGTTTTTGCATTGGTACACAGGGGAGGGAATGGATGAGATGGAGTTCACTGAGGCTGAGAGTAATATGAATGATTTGGTATCAGAGTACCAGCAGTATCAAGATGCTGTGGCAGAGGATGAGGAGGAGGTGGAAGAAGATGTGTTGTAGCGCTAATAAGAGACGAGGAAATTTGAAAATGTATCCTGATGAATCTCTGTTTGTGTTGCCAAGTATATAGGCTTTGGAGTGAATAAGTTCTTGTTTGATGAGATGGGTTTGGGTGTATTGATGTTCTAAATTTGTTGTTGCATTAAATTATTGACATATGGGATGAGTTCTGTAAGTATGAAAGGTTGAATGAAATGTTTGAGATTCCTTGCAGTAAACAGTTTCTATAAAATAAATTTTGGTTATTTTCACAGATGTGTGAAATAGACCTTTCAGAAAACACAACCTAACCAGGGTGG

mRNA sequence

ACAAGAACTAGCAGCTCTCCTCTTCTTGTCACCTTCCCTTGTCATCCAGCTCACACAACTCCAATTTGGAATTTGACAGGTCTCTCCTCACTCATACATTCCCACATGAAATTATTAATTGAATCTTCAACCATGTCTATGATTCTTCAGCTTCACTGTCCTTTTCCACCATTTTTTTCTTCAAGATAAAGACTAGTAAACTCCTTATATATCCCTCTGCCTCTCTTCCCATTCATCTGTGCAAATTCACAAACCATCTCCCATTTCCTTCTTGTTTATCTCTGTTTTTTTATTTTACACATCTGTTGAACTTCCCATCTGAAAAATGAGAGAAATTCTCCATATTCAAGCAGGACAGTGTGGAAACCAAATTGGAGCTAAGTTTTGGGAAGTTGTTTGTGATGAACATGGGATTGATCCTGCTGGAAATTACACCGGAAGTAGTAGCCTTCAACTCGAGAGGGTTAATGTTTACTACAACGAAGCGAGTGGGGGACGATATGTTCCAAGGGCTGTTTTGGTTGATCTTGAACCAGGAACCATGGACAGCCTCAGGACAGGTCCTTATGGGAATATATTTAGACCTGATAACTTTGTTTTTGGGCAAAATGGGGCTGGAAACAACTGGGCTAAGGGTCATTACACTGAAGGAGCTGAACTGGTTGATTCTGTTCTTGATGTGGTTCGCAAAGAAGCTGAGAATTGTGACTGTTTGCAAGGGTTTCAAGTTTGCCATTCACTTGGAGGAGGGACTGGATCTGGAATGGGAACTCTATTGATATCAAAGATCAGGGAAGAGTATCCTGACAGAATGATGTTAACTTTCTCTGTCTTCCCCTCACCTAAGGTCTCGGATACAGTGGTTGAGCCCTATAATGCCACTCTCTCTGTGCACCAGTTGGTTGAAAATGCTGATGAATGCATGGTTTTGGATAATGAAGCACTCTATGACATCTGCTTTAGGACCCTCAAGCTCACCAATCCAAGTTTTGGTGATCTCAACCACTTGATTTCTACAACCATGAGTGGAGTTACATGCTGCCTTCGCTTCCCTGGCCAGCTCAACTCCGACCTCCGGAAGCTAGCTGTGAACCTCATCCCTTTCCCGCGCCTCCACTTCTTCATGGTCGGTTTTGCTCCCTTGACTGCTCGTGGCTCACAGCAGTACCGTGCACTCACCATCCCTGAGCTCACCCAACAAATGTGGGATGCCAAAAACATGATGTGTGCTGCTGATCCTCGCCATGGTCGATACCTAACTGCCTCAGCTTTATTCCGTGGCAAGATGAGCACCAAGGAAGTCGATGAACAAATGATAAATGTACAGAACAAGAACTCATCTTATTTTGTTGAGTGGATCCCAAACAATGTTAAATCAAGTGTTTGTGACATCCCTCCAACTGGGTTGTCAATGTCTTCAACATTCATGGGAAACTCGACCTCAATACAAGAGATGTTTCGGCGAGTTTCTGAACAATTCACGGTCATGTTCAGGAGGAAGGCGTTTTTGCATTGGTACACAGGGGAGGGAATGGATGAGATGGAGTTCACTGAGGCTGAGAGTAATATGAATGATTTGGTATCAGAGTACCAGCAGTATCAAGATGCTGTGGCAGAGGATGAGGAGGAGGTGGAAGAAGATGTGTTGTAGCGCTAATAAGAGACGAGGAAATTTGAAAATGTATCCTGATGAATCTCTGTTTGTGTTGCCAAGTATATAGGCTTTGGAGTGAATAAGTTCTTGTTTGATGAGATGGGTTTGGGTGTATTGATGTTCTAAATTTGTTGTTGCATTAAATTATTGACATATGGGATGAGTTCTGTAAGTATGAAAGGTTGAATGAAATGTTTGAGATTCCTTGCAGTAAACAGTTTCTATAAAATAAATTTTGGTTATTTTCACAGATGTGTGAAATAGACCTTTCAGAAAACACAACCTAACCAGGGTGG

Coding sequence (CDS)

ATGAGAGAAATTCTCCATATTCAAGCAGGACAGTGTGGAAACCAAATTGGAGCTAAGTTTTGGGAAGTTGTTTGTGATGAACATGGGATTGATCCTGCTGGAAATTACACCGGAAGTAGTAGCCTTCAACTCGAGAGGGTTAATGTTTACTACAACGAAGCGAGTGGGGGACGATATGTTCCAAGGGCTGTTTTGGTTGATCTTGAACCAGGAACCATGGACAGCCTCAGGACAGGTCCTTATGGGAATATATTTAGACCTGATAACTTTGTTTTTGGGCAAAATGGGGCTGGAAACAACTGGGCTAAGGGTCATTACACTGAAGGAGCTGAACTGGTTGATTCTGTTCTTGATGTGGTTCGCAAAGAAGCTGAGAATTGTGACTGTTTGCAAGGGTTTCAAGTTTGCCATTCACTTGGAGGAGGGACTGGATCTGGAATGGGAACTCTATTGATATCAAAGATCAGGGAAGAGTATCCTGACAGAATGATGTTAACTTTCTCTGTCTTCCCCTCACCTAAGGTCTCGGATACAGTGGTTGAGCCCTATAATGCCACTCTCTCTGTGCACCAGTTGGTTGAAAATGCTGATGAATGCATGGTTTTGGATAATGAAGCACTCTATGACATCTGCTTTAGGACCCTCAAGCTCACCAATCCAAGTTTTGGTGATCTCAACCACTTGATTTCTACAACCATGAGTGGAGTTACATGCTGCCTTCGCTTCCCTGGCCAGCTCAACTCCGACCTCCGGAAGCTAGCTGTGAACCTCATCCCTTTCCCGCGCCTCCACTTCTTCATGGTCGGTTTTGCTCCCTTGACTGCTCGTGGCTCACAGCAGTACCGTGCACTCACCATCCCTGAGCTCACCCAACAAATGTGGGATGCCAAAAACATGATGTGTGCTGCTGATCCTCGCCATGGTCGATACCTAACTGCCTCAGCTTTATTCCGTGGCAAGATGAGCACCAAGGAAGTCGATGAACAAATGATAAATGTACAGAACAAGAACTCATCTTATTTTGTTGAGTGGATCCCAAACAATGTTAAATCAAGTGTTTGTGACATCCCTCCAACTGGGTTGTCAATGTCTTCAACATTCATGGGAAACTCGACCTCAATACAAGAGATGTTTCGGCGAGTTTCTGAACAATTCACGGTCATGTTCAGGAGGAAGGCGTTTTTGCATTGGTACACAGGGGAGGGAATGGATGAGATGGAGTTCACTGAGGCTGAGAGTAATATGAATGATTTGGTATCAGAGTACCAGCAGTATCAAGATGCTGTGGCAGAGGATGAGGAGGAGGTGGAAGAAGATGTGTTGTAG

Protein sequence

MREILHIQAGQCGNQIGAKFWEVVCDEHGIDPAGNYTGSSSLQLERVNVYYNEASGGRYVPRAVLVDLEPGTMDSLRTGPYGNIFRPDNFVFGQNGAGNNWAKGHYTEGAELVDSVLDVVRKEAENCDCLQGFQVCHSLGGGTGSGMGTLLISKIREEYPDRMMLTFSVFPSPKVSDTVVEPYNATLSVHQLVENADECMVLDNEALYDICFRTLKLTNPSFGDLNHLISTTMSGVTCCLRFPGQLNSDLRKLAVNLIPFPRLHFFMVGFAPLTARGSQQYRALTIPELTQQMWDAKNMMCAADPRHGRYLTASALFRGKMSTKEVDEQMINVQNKNSSYFVEWIPNNVKSSVCDIPPTGLSMSSTFMGNSTSIQEMFRRVSEQFTVMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVSEYQQYQDAVAEDEEEVEEDVL*
Homology
BLAST of CSPI05G05440 vs. ExPASy Swiss-Prot
Match: P93176 (Tubulin beta chain OS=Hordeum vulgare OX=4513 GN=TUBB PE=2 SV=1)

HSP 1 Score: 860.1 bits (2221), Expect = 1.1e-248
Identity = 412/439 (93.85%), Postives = 427/439 (97.27%), Query Frame = 0

Query: 1   MREILHIQAGQCGNQIGAKFWEVVCDEHGIDPAGNYTGSSSLQLERVNVYYNEASGGRYV 60
           MREILHIQ GQCGNQIGAKFWEVVCDEHGIDP G YTG+S LQLERVNVYYNEAS GR+V
Sbjct: 1   MREILHIQGGQCGNQIGAKFWEVVCDEHGIDPTGRYTGTSDLQLERVNVYYNEASCGRFV 60

Query: 61  PRAVLVDLEPGTMDSLRTGPYGNIFRPDNFVFGQNGAGNNWAKGHYTEGAELVDSVLDVV 120
           PRAVL+DLEPGTMDS+RTGPYG IFRPDNFVFGQ+GAGNNWAKGHYTEGAEL+DSVLDVV
Sbjct: 61  PRAVLMDLEPGTMDSVRTGPYGQIFRPDNFVFGQSGAGNNWAKGHYTEGAELIDSVLDVV 120

Query: 121 RKEAENCDCLQGFQVCHSLGGGTGSGMGTLLISKIREEYPDRMMLTFSVFPSPKVSDTVV 180
           RKEAENCDCLQGFQVCHSLGGGTGSGMGTLLISKIREEYPDRMMLTFSVFPSPKVSDTVV
Sbjct: 121 RKEAENCDCLQGFQVCHSLGGGTGSGMGTLLISKIREEYPDRMMLTFSVFPSPKVSDTVV 180

Query: 181 EPYNATLSVHQLVENADECMVLDNEALYDICFRTLKLTNPSFGDLNHLISTTMSGVTCCL 240
           EPYNATLSVHQLVENADECMVLDNEALYDICFRTLKLT PSFGDLNHLIS TMSGVTCCL
Sbjct: 181 EPYNATLSVHQLVENADECMVLDNEALYDICFRTLKLTTPSFGDLNHLISATMSGVTCCL 240

Query: 241 RFPGQLNSDLRKLAVNLIPFPRLHFFMVGFAPLTARGSQQYRALTIPELTQQMWDAKNMM 300
           RFPGQLNSDLRKLAVNLIPFPRLHFFMVGFAPLT+RGSQQYRALT+PELTQQMWDAKNMM
Sbjct: 241 RFPGQLNSDLRKLAVNLIPFPRLHFFMVGFAPLTSRGSQQYRALTVPELTQQMWDAKNMM 300

Query: 301 CAADPRHGRYLTASALFRGKMSTKEVDEQMINVQNKNSSYFVEWIPNNVKSSVCDIPPTG 360
           CAADPRHGRYLTASA+FRGKMSTKEVDEQMINVQNKNSSYFVEWIPNNVKSSVCDIPPTG
Sbjct: 301 CAADPRHGRYLTASAMFRGKMSTKEVDEQMINVQNKNSSYFVEWIPNNVKSSVCDIPPTG 360

Query: 361 LSMSSTFMGNSTSIQEMFRRVSEQFTVMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVS 420
           LSM+STF+GNSTSIQEMFRRVSEQFT MFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVS
Sbjct: 361 LSMASTFVGNSTSIQEMFRRVSEQFTAMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVS 420

Query: 421 EYQQYQDAVAEDEEEVEED 440
           EYQQYQDA A++E E E++
Sbjct: 421 EYQQYQDATADEEGEYEDE 439

BLAST of CSPI05G05440 vs. ExPASy Swiss-Prot
Match: Q9ZRB1 (Tubulin beta-2 chain OS=Triticum aestivum OX=4565 GN=TUBB2 PE=2 SV=1)

HSP 1 Score: 858.2 bits (2216), Expect = 4.1e-248
Identity = 411/439 (93.62%), Postives = 427/439 (97.27%), Query Frame = 0

Query: 1   MREILHIQAGQCGNQIGAKFWEVVCDEHGIDPAGNYTGSSSLQLERVNVYYNEASGGRYV 60
           MREILHIQ GQCGNQIGAKFWEVVCDEHGIDP G YTG+S LQLERVNVYYNEAS GR+V
Sbjct: 1   MREILHIQGGQCGNQIGAKFWEVVCDEHGIDPTGRYTGTSDLQLERVNVYYNEASCGRFV 60

Query: 61  PRAVLVDLEPGTMDSLRTGPYGNIFRPDNFVFGQNGAGNNWAKGHYTEGAELVDSVLDVV 120
           PRAVL+DLEPGTMDS+RTGPYG IFRPDNFVFGQ+GAGNNWAKGHYTEGAEL+DSVLDVV
Sbjct: 61  PRAVLMDLEPGTMDSVRTGPYGQIFRPDNFVFGQSGAGNNWAKGHYTEGAELIDSVLDVV 120

Query: 121 RKEAENCDCLQGFQVCHSLGGGTGSGMGTLLISKIREEYPDRMMLTFSVFPSPKVSDTVV 180
           RKEAENCDCLQGFQVCHSLGGGTGSGMGTLLISKIREEYPDRMMLTFSVFPSPKVSDTVV
Sbjct: 121 RKEAENCDCLQGFQVCHSLGGGTGSGMGTLLISKIREEYPDRMMLTFSVFPSPKVSDTVV 180

Query: 181 EPYNATLSVHQLVENADECMVLDNEALYDICFRTLKLTNPSFGDLNHLISTTMSGVTCCL 240
           EPYNATLSVHQLVENADECMVLDNEALYDICFRTLKLT PSFGDLNHLIS TMSGVTCCL
Sbjct: 181 EPYNATLSVHQLVENADECMVLDNEALYDICFRTLKLTTPSFGDLNHLISATMSGVTCCL 240

Query: 241 RFPGQLNSDLRKLAVNLIPFPRLHFFMVGFAPLTARGSQQYRALTIPELTQQMWDAKNMM 300
           RFPGQLNSDLRKLAVNLIPFPRLHFFMVGFAPLT+RGSQQYRALT+PELTQQMWDAKNMM
Sbjct: 241 RFPGQLNSDLRKLAVNLIPFPRLHFFMVGFAPLTSRGSQQYRALTVPELTQQMWDAKNMM 300

Query: 301 CAADPRHGRYLTASALFRGKMSTKEVDEQMINVQNKNSSYFVEWIPNNVKSSVCDIPPTG 360
           CAADPRHGRYLTASA+FRGKMSTKEVDEQMINVQNKNSSYFVEWIPNNVKSSVCDIPPTG
Sbjct: 301 CAADPRHGRYLTASAMFRGKMSTKEVDEQMINVQNKNSSYFVEWIPNNVKSSVCDIPPTG 360

Query: 361 LSMSSTFMGNSTSIQEMFRRVSEQFTVMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVS 420
           LSM+STF+GNSTSIQEMFRRVSEQFT MFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVS
Sbjct: 361 LSMASTFVGNSTSIQEMFRRVSEQFTSMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVS 420

Query: 421 EYQQYQDAVAEDEEEVEED 440
           EYQQYQDA +++E E E++
Sbjct: 421 EYQQYQDATSDEEGEYEDE 439

BLAST of CSPI05G05440 vs. ExPASy Swiss-Prot
Match: Q41783 (Tubulin beta-6 chain OS=Zea mays OX=4577 GN=TUBB6 PE=2 SV=1)

HSP 1 Score: 858.2 bits (2216), Expect = 4.1e-248
Identity = 415/444 (93.47%), Postives = 427/444 (96.17%), Query Frame = 0

Query: 1   MREILHIQAGQCGNQIGAKFWEVVCDEHGIDPAGNYTGSSSLQLERVNVYYNEASGGRYV 60
           MREILHIQ GQCGNQIGAKFWEVVCDEHGIDP G YTG+S LQLERVNVYYNEAS GR+V
Sbjct: 1   MREILHIQGGQCGNQIGAKFWEVVCDEHGIDPTGRYTGNSDLQLERVNVYYNEASCGRFV 60

Query: 61  PRAVLVDLEPGTMDSLRTGPYGNIFRPDNFVFGQNGAGNNWAKGHYTEGAELVDSVLDVV 120
           PRAVL+DLEPGTMDS+RTGPYG IFRPDNFVFGQ+GAGNNWAKGHYTEGAEL+DSVLDVV
Sbjct: 61  PRAVLMDLEPGTMDSVRTGPYGQIFRPDNFVFGQSGAGNNWAKGHYTEGAELIDSVLDVV 120

Query: 121 RKEAENCDCLQGFQVCHSLGGGTGSGMGTLLISKIREEYPDRMMLTFSVFPSPKVSDTVV 180
           RKEAENCDCLQGFQVCHSLGGGTGSGMGTLLISKIREEYPDRMMLTFSVFPSPKVSDTVV
Sbjct: 121 RKEAENCDCLQGFQVCHSLGGGTGSGMGTLLISKIREEYPDRMMLTFSVFPSPKVSDTVV 180

Query: 181 EPYNATLSVHQLVENADECMVLDNEALYDICFRTLKLTNPSFGDLNHLISTTMSGVTCCL 240
           EPYNATLSVHQLVENADECMVLDNEALYDICFRTLKLT PSFGDLNHLIS TMSGVTCCL
Sbjct: 181 EPYNATLSVHQLVENADECMVLDNEALYDICFRTLKLTTPSFGDLNHLISATMSGVTCCL 240

Query: 241 RFPGQLNSDLRKLAVNLIPFPRLHFFMVGFAPLTARGSQQYRALTIPELTQQMWDAKNMM 300
           RFPGQLNSDLRKLAVNLIPFPRLHFFMVGFAPLT+RGSQQYRALT+PELTQQMWDAKNMM
Sbjct: 241 RFPGQLNSDLRKLAVNLIPFPRLHFFMVGFAPLTSRGSQQYRALTVPELTQQMWDAKNMM 300

Query: 301 CAADPRHGRYLTASALFRGKMSTKEVDEQMINVQNKNSSYFVEWIPNNVKSSVCDIPPTG 360
           CAADPRHGRYLTASA+FRGKMSTKEVDEQMINVQNKNSSYFVEWIPNNVKSSVCDIPP G
Sbjct: 301 CAADPRHGRYLTASAMFRGKMSTKEVDEQMINVQNKNSSYFVEWIPNNVKSSVCDIPPRG 360

Query: 361 LSMSSTFMGNSTSIQEMFRRVSEQFTVMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVS 420
           LSM+STF+GNSTSIQEMFRRVSEQFT MFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVS
Sbjct: 361 LSMASTFIGNSTSIQEMFRRVSEQFTAMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVS 420

Query: 421 EYQQYQDAVA-----EDEEEVEED 440
           EYQQYQDA A     EDEEEV+ D
Sbjct: 421 EYQQYQDATADEEEYEDEEEVQAD 444

BLAST of CSPI05G05440 vs. ExPASy Swiss-Prot
Match: P45960 (Tubulin beta-4 chain OS=Oryza sativa subsp. japonica OX=39947 GN=TUBB4 PE=2 SV=1)

HSP 1 Score: 857.8 bits (2215), Expect = 5.3e-248
Identity = 411/439 (93.62%), Postives = 426/439 (97.04%), Query Frame = 0

Query: 1   MREILHIQAGQCGNQIGAKFWEVVCDEHGIDPAGNYTGSSSLQLERVNVYYNEASGGRYV 60
           MREILHIQ GQCGNQIGAKFWEVVCDEHGIDP G YTG+S LQLERVNVYYNEAS GR+V
Sbjct: 1   MREILHIQGGQCGNQIGAKFWEVVCDEHGIDPTGRYTGNSDLQLERVNVYYNEASCGRFV 60

Query: 61  PRAVLVDLEPGTMDSLRTGPYGNIFRPDNFVFGQNGAGNNWAKGHYTEGAELVDSVLDVV 120
           PRAVL+DLEPGTMDS+RTGPYG IFRPDNFVFGQ+GAGNNWAKGHYTEGAEL+DSVLDVV
Sbjct: 61  PRAVLMDLEPGTMDSVRTGPYGQIFRPDNFVFGQSGAGNNWAKGHYTEGAELIDSVLDVV 120

Query: 121 RKEAENCDCLQGFQVCHSLGGGTGSGMGTLLISKIREEYPDRMMLTFSVFPSPKVSDTVV 180
           RKEAENCDCLQGFQVCHSLGGGTGSGMGTLLISKIREEYPDRMMLTFSVFPSPKVSDTVV
Sbjct: 121 RKEAENCDCLQGFQVCHSLGGGTGSGMGTLLISKIREEYPDRMMLTFSVFPSPKVSDTVV 180

Query: 181 EPYNATLSVHQLVENADECMVLDNEALYDICFRTLKLTNPSFGDLNHLISTTMSGVTCCL 240
           EPYNATLSVHQLVENADECMVLDNEALYDICFRTLKLT PSFGDLNHLIS TMSGVTCCL
Sbjct: 181 EPYNATLSVHQLVENADECMVLDNEALYDICFRTLKLTTPSFGDLNHLISATMSGVTCCL 240

Query: 241 RFPGQLNSDLRKLAVNLIPFPRLHFFMVGFAPLTARGSQQYRALTIPELTQQMWDAKNMM 300
           RFPGQLNSDLRKLAVNLIPFPRLHFFMVGFAPLT+RGSQQYRALT+PELTQQMWDAKNMM
Sbjct: 241 RFPGQLNSDLRKLAVNLIPFPRLHFFMVGFAPLTSRGSQQYRALTVPELTQQMWDAKNMM 300

Query: 301 CAADPRHGRYLTASALFRGKMSTKEVDEQMINVQNKNSSYFVEWIPNNVKSSVCDIPPTG 360
           CAADPRHGRYLTASA+FRGKMSTKEVDEQMINVQNKNSSYFVEWIPNNVKSSVCDIPP G
Sbjct: 301 CAADPRHGRYLTASAMFRGKMSTKEVDEQMINVQNKNSSYFVEWIPNNVKSSVCDIPPRG 360

Query: 361 LSMSSTFMGNSTSIQEMFRRVSEQFTVMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVS 420
           LSM+STF+GNSTSIQEMFRRVSEQFT MFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVS
Sbjct: 361 LSMASTFIGNSTSIQEMFRRVSEQFTAMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVS 420

Query: 421 EYQQYQDAVAEDEEEVEED 440
           EYQQYQDA A++E E E++
Sbjct: 421 EYQQYQDATADEEGEYEDE 439

BLAST of CSPI05G05440 vs. ExPASy Swiss-Prot
Match: Q9ZPN9 (Tubulin beta-2 chain OS=Eleusine indica OX=29674 GN=TUBB2 PE=2 SV=1)

HSP 1 Score: 857.1 bits (2213), Expect = 9.0e-248
Identity = 410/440 (93.18%), Postives = 427/440 (97.05%), Query Frame = 0

Query: 1   MREILHIQAGQCGNQIGAKFWEVVCDEHGIDPAGNYTGSSSLQLERVNVYYNEASGGRYV 60
           MREILHIQ GQCGNQIGAKFWEVVCDEHGIDP G YTG+S LQLERVNVYYNEAS GR+V
Sbjct: 1   MREILHIQGGQCGNQIGAKFWEVVCDEHGIDPTGRYTGTSDLQLERVNVYYNEASCGRFV 60

Query: 61  PRAVLVDLEPGTMDSLRTGPYGNIFRPDNFVFGQNGAGNNWAKGHYTEGAELVDSVLDVV 120
           PRAVL+DLEPGTMDS+RTGPYG IFRPDNFVFGQ+GAGNNWAKGHYTEGAEL+DSVLDVV
Sbjct: 61  PRAVLMDLEPGTMDSVRTGPYGQIFRPDNFVFGQSGAGNNWAKGHYTEGAELIDSVLDVV 120

Query: 121 RKEAENCDCLQGFQVCHSLGGGTGSGMGTLLISKIREEYPDRMMLTFSVFPSPKVSDTVV 180
           RKEAENCDCLQGFQVCHSLGGGTGSGMGTLLISKIREEYPDRMMLTFSVFPSPKVSDTVV
Sbjct: 121 RKEAENCDCLQGFQVCHSLGGGTGSGMGTLLISKIREEYPDRMMLTFSVFPSPKVSDTVV 180

Query: 181 EPYNATLSVHQLVENADECMVLDNEALYDICFRTLKLTNPSFGDLNHLISTTMSGVTCCL 240
           EPYNATLSVHQLVENADECMVLDNEALYDICFRTLKLT PSFGDLNHLIS TMSGVTCCL
Sbjct: 181 EPYNATLSVHQLVENADECMVLDNEALYDICFRTLKLTTPSFGDLNHLISATMSGVTCCL 240

Query: 241 RFPGQLNSDLRKLAVNLIPFPRLHFFMVGFAPLTARGSQQYRALTIPELTQQMWDAKNMM 300
           RFPGQLNSDLRKLAVNLIPFPRLHFFMVGFAPLT+RGSQQYRALT+PELTQQMWDAKNMM
Sbjct: 241 RFPGQLNSDLRKLAVNLIPFPRLHFFMVGFAPLTSRGSQQYRALTVPELTQQMWDAKNMM 300

Query: 301 CAADPRHGRYLTASALFRGKMSTKEVDEQMINVQNKNSSYFVEWIPNNVKSSVCDIPPTG 360
           CAADPRHGRYLTASA+FRGKMSTKEVDEQMINVQNKNSSYFVEWIPNNVKSSVCDIPP G
Sbjct: 301 CAADPRHGRYLTASAMFRGKMSTKEVDEQMINVQNKNSSYFVEWIPNNVKSSVCDIPPRG 360

Query: 361 LSMSSTFMGNSTSIQEMFRRVSEQFTVMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVS 420
           LSM+STF+GNSTSIQEMFRRVSEQFT MFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVS
Sbjct: 361 LSMASTFIGNSTSIQEMFRRVSEQFTAMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVS 420

Query: 421 EYQQYQDAVAEDEEEVEEDV 441
           EYQQYQDA A+++ E E+++
Sbjct: 421 EYQQYQDATADEDGEYEDEL 440

BLAST of CSPI05G05440 vs. ExPASy TrEMBL
Match: A0A0A0KQW7 (Tubulin beta chain OS=Cucumis sativus OX=3659 GN=Csa_5G153160 PE=3 SV=1)

HSP 1 Score: 906.0 bits (2340), Expect = 6.3e-260
Identity = 441/441 (100.00%), Postives = 441/441 (100.00%), Query Frame = 0

Query: 1   MREILHIQAGQCGNQIGAKFWEVVCDEHGIDPAGNYTGSSSLQLERVNVYYNEASGGRYV 60
           MREILHIQAGQCGNQIGAKFWEVVCDEHGIDPAGNYTGSSSLQLERVNVYYNEASGGRYV
Sbjct: 1   MREILHIQAGQCGNQIGAKFWEVVCDEHGIDPAGNYTGSSSLQLERVNVYYNEASGGRYV 60

Query: 61  PRAVLVDLEPGTMDSLRTGPYGNIFRPDNFVFGQNGAGNNWAKGHYTEGAELVDSVLDVV 120
           PRAVLVDLEPGTMDSLRTGPYGNIFRPDNFVFGQNGAGNNWAKGHYTEGAELVDSVLDVV
Sbjct: 61  PRAVLVDLEPGTMDSLRTGPYGNIFRPDNFVFGQNGAGNNWAKGHYTEGAELVDSVLDVV 120

Query: 121 RKEAENCDCLQGFQVCHSLGGGTGSGMGTLLISKIREEYPDRMMLTFSVFPSPKVSDTVV 180
           RKEAENCDCLQGFQVCHSLGGGTGSGMGTLLISKIREEYPDRMMLTFSVFPSPKVSDTVV
Sbjct: 121 RKEAENCDCLQGFQVCHSLGGGTGSGMGTLLISKIREEYPDRMMLTFSVFPSPKVSDTVV 180

Query: 181 EPYNATLSVHQLVENADECMVLDNEALYDICFRTLKLTNPSFGDLNHLISTTMSGVTCCL 240
           EPYNATLSVHQLVENADECMVLDNEALYDICFRTLKLTNPSFGDLNHLISTTMSGVTCCL
Sbjct: 181 EPYNATLSVHQLVENADECMVLDNEALYDICFRTLKLTNPSFGDLNHLISTTMSGVTCCL 240

Query: 241 RFPGQLNSDLRKLAVNLIPFPRLHFFMVGFAPLTARGSQQYRALTIPELTQQMWDAKNMM 300
           RFPGQLNSDLRKLAVNLIPFPRLHFFMVGFAPLTARGSQQYRALTIPELTQQMWDAKNMM
Sbjct: 241 RFPGQLNSDLRKLAVNLIPFPRLHFFMVGFAPLTARGSQQYRALTIPELTQQMWDAKNMM 300

Query: 301 CAADPRHGRYLTASALFRGKMSTKEVDEQMINVQNKNSSYFVEWIPNNVKSSVCDIPPTG 360
           CAADPRHGRYLTASALFRGKMSTKEVDEQMINVQNKNSSYFVEWIPNNVKSSVCDIPPTG
Sbjct: 301 CAADPRHGRYLTASALFRGKMSTKEVDEQMINVQNKNSSYFVEWIPNNVKSSVCDIPPTG 360

Query: 361 LSMSSTFMGNSTSIQEMFRRVSEQFTVMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVS 420
           LSMSSTFMGNSTSIQEMFRRVSEQFTVMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVS
Sbjct: 361 LSMSSTFMGNSTSIQEMFRRVSEQFTVMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVS 420

Query: 421 EYQQYQDAVAEDEEEVEEDVL 442
           EYQQYQDAVAEDEEEVEEDVL
Sbjct: 421 EYQQYQDAVAEDEEEVEEDVL 441

BLAST of CSPI05G05440 vs. ExPASy TrEMBL
Match: A0A5A7THJ1 (Tubulin beta chain OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold44G002600 PE=3 SV=1)

HSP 1 Score: 903.3 bits (2333), Expect = 4.1e-259
Identity = 440/441 (99.77%), Postives = 440/441 (99.77%), Query Frame = 0

Query: 1   MREILHIQAGQCGNQIGAKFWEVVCDEHGIDPAGNYTGSSSLQLERVNVYYNEASGGRYV 60
           MREILHIQAGQCGNQIGAKFWEVVCDEHGIDPAGNYTGSSSLQLERVNVYYNEASGGRYV
Sbjct: 1   MREILHIQAGQCGNQIGAKFWEVVCDEHGIDPAGNYTGSSSLQLERVNVYYNEASGGRYV 60

Query: 61  PRAVLVDLEPGTMDSLRTGPYGNIFRPDNFVFGQNGAGNNWAKGHYTEGAELVDSVLDVV 120
           PRAVLVDLEPGTMDSLRTGPYGNIFRPDNFVFGQNGAGNNWAKGHYTEGAELVDSVLDVV
Sbjct: 61  PRAVLVDLEPGTMDSLRTGPYGNIFRPDNFVFGQNGAGNNWAKGHYTEGAELVDSVLDVV 120

Query: 121 RKEAENCDCLQGFQVCHSLGGGTGSGMGTLLISKIREEYPDRMMLTFSVFPSPKVSDTVV 180
           RKEAENCDCLQGFQVCHSLGGGTGSGMGTLLISKIREEYPDRMMLTFSVFPSPKVSDTVV
Sbjct: 121 RKEAENCDCLQGFQVCHSLGGGTGSGMGTLLISKIREEYPDRMMLTFSVFPSPKVSDTVV 180

Query: 181 EPYNATLSVHQLVENADECMVLDNEALYDICFRTLKLTNPSFGDLNHLISTTMSGVTCCL 240
           EPYNATLSVHQLVENADECMVLDNEALYDICFRTLKLTNPSFGDLNHLISTTMSGVTCCL
Sbjct: 181 EPYNATLSVHQLVENADECMVLDNEALYDICFRTLKLTNPSFGDLNHLISTTMSGVTCCL 240

Query: 241 RFPGQLNSDLRKLAVNLIPFPRLHFFMVGFAPLTARGSQQYRALTIPELTQQMWDAKNMM 300
           RFPGQLNSDLRKLAVNLIPFPRLHFFMVGFAPLTARGSQQYRALTIPELTQQMWDAKNMM
Sbjct: 241 RFPGQLNSDLRKLAVNLIPFPRLHFFMVGFAPLTARGSQQYRALTIPELTQQMWDAKNMM 300

Query: 301 CAADPRHGRYLTASALFRGKMSTKEVDEQMINVQNKNSSYFVEWIPNNVKSSVCDIPPTG 360
           CAADPRHGRYLTASALFRGKMSTKEVDEQMINVQNKNSSYFVEWIPNNVKSSVCDIPPTG
Sbjct: 301 CAADPRHGRYLTASALFRGKMSTKEVDEQMINVQNKNSSYFVEWIPNNVKSSVCDIPPTG 360

Query: 361 LSMSSTFMGNSTSIQEMFRRVSEQFTVMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVS 420
           LSMSSTFMGNSTSIQEMFRRVSEQFTVMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVS
Sbjct: 361 LSMSSTFMGNSTSIQEMFRRVSEQFTVMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVS 420

Query: 421 EYQQYQDAVAEDEEEVEEDVL 442
           EYQQYQDAVAEDEEE EEDVL
Sbjct: 421 EYQQYQDAVAEDEEEGEEDVL 441

BLAST of CSPI05G05440 vs. ExPASy TrEMBL
Match: A0A1S3ATL6 (Tubulin beta chain OS=Cucumis melo OX=3656 GN=LOC103482745 PE=3 SV=1)

HSP 1 Score: 903.3 bits (2333), Expect = 4.1e-259
Identity = 440/441 (99.77%), Postives = 440/441 (99.77%), Query Frame = 0

Query: 1   MREILHIQAGQCGNQIGAKFWEVVCDEHGIDPAGNYTGSSSLQLERVNVYYNEASGGRYV 60
           MREILHIQAGQCGNQIGAKFWEVVCDEHGIDPAGNYTGSSSLQLERVNVYYNEASGGRYV
Sbjct: 1   MREILHIQAGQCGNQIGAKFWEVVCDEHGIDPAGNYTGSSSLQLERVNVYYNEASGGRYV 60

Query: 61  PRAVLVDLEPGTMDSLRTGPYGNIFRPDNFVFGQNGAGNNWAKGHYTEGAELVDSVLDVV 120
           PRAVLVDLEPGTMDSLRTGPYGNIFRPDNFVFGQNGAGNNWAKGHYTEGAELVDSVLDVV
Sbjct: 61  PRAVLVDLEPGTMDSLRTGPYGNIFRPDNFVFGQNGAGNNWAKGHYTEGAELVDSVLDVV 120

Query: 121 RKEAENCDCLQGFQVCHSLGGGTGSGMGTLLISKIREEYPDRMMLTFSVFPSPKVSDTVV 180
           RKEAENCDCLQGFQVCHSLGGGTGSGMGTLLISKIREEYPDRMMLTFSVFPSPKVSDTVV
Sbjct: 121 RKEAENCDCLQGFQVCHSLGGGTGSGMGTLLISKIREEYPDRMMLTFSVFPSPKVSDTVV 180

Query: 181 EPYNATLSVHQLVENADECMVLDNEALYDICFRTLKLTNPSFGDLNHLISTTMSGVTCCL 240
           EPYNATLSVHQLVENADECMVLDNEALYDICFRTLKLTNPSFGDLNHLISTTMSGVTCCL
Sbjct: 181 EPYNATLSVHQLVENADECMVLDNEALYDICFRTLKLTNPSFGDLNHLISTTMSGVTCCL 240

Query: 241 RFPGQLNSDLRKLAVNLIPFPRLHFFMVGFAPLTARGSQQYRALTIPELTQQMWDAKNMM 300
           RFPGQLNSDLRKLAVNLIPFPRLHFFMVGFAPLTARGSQQYRALTIPELTQQMWDAKNMM
Sbjct: 241 RFPGQLNSDLRKLAVNLIPFPRLHFFMVGFAPLTARGSQQYRALTIPELTQQMWDAKNMM 300

Query: 301 CAADPRHGRYLTASALFRGKMSTKEVDEQMINVQNKNSSYFVEWIPNNVKSSVCDIPPTG 360
           CAADPRHGRYLTASALFRGKMSTKEVDEQMINVQNKNSSYFVEWIPNNVKSSVCDIPPTG
Sbjct: 301 CAADPRHGRYLTASALFRGKMSTKEVDEQMINVQNKNSSYFVEWIPNNVKSSVCDIPPTG 360

Query: 361 LSMSSTFMGNSTSIQEMFRRVSEQFTVMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVS 420
           LSMSSTFMGNSTSIQEMFRRVSEQFTVMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVS
Sbjct: 361 LSMSSTFMGNSTSIQEMFRRVSEQFTVMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVS 420

Query: 421 EYQQYQDAVAEDEEEVEEDVL 442
           EYQQYQDAVAEDEEE EEDVL
Sbjct: 421 EYQQYQDAVAEDEEEGEEDVL 441

BLAST of CSPI05G05440 vs. ExPASy TrEMBL
Match: A0A6J1DZ61 (Tubulin beta chain OS=Momordica charantia OX=3673 GN=LOC111025947 PE=3 SV=1)

HSP 1 Score: 894.4 bits (2310), Expect = 1.9e-256
Identity = 435/441 (98.64%), Postives = 437/441 (99.09%), Query Frame = 0

Query: 1   MREILHIQAGQCGNQIGAKFWEVVCDEHGIDPAGNYTGSSSLQLERVNVYYNEASGGRYV 60
           MREILH+QAGQCGNQIGAKFWEVVCDEHGIDP GNY GSSS+QLERVNVYYNEASGGRYV
Sbjct: 1   MREILHVQAGQCGNQIGAKFWEVVCDEHGIDPTGNYVGSSSIQLERVNVYYNEASGGRYV 60

Query: 61  PRAVLVDLEPGTMDSLRTGPYGNIFRPDNFVFGQNGAGNNWAKGHYTEGAELVDSVLDVV 120
           PRAVLVDLEPGTMDSLRTGPYGNIFRPDNFVFGQNGAGNNWAKGHYTEGAELVDSVLDVV
Sbjct: 61  PRAVLVDLEPGTMDSLRTGPYGNIFRPDNFVFGQNGAGNNWAKGHYTEGAELVDSVLDVV 120

Query: 121 RKEAENCDCLQGFQVCHSLGGGTGSGMGTLLISKIREEYPDRMMLTFSVFPSPKVSDTVV 180
           RKEAEN DCLQGFQVCHSLGGGTGSGMGTLLISKIREEYPDRMMLTFSVFPSPKVSDTVV
Sbjct: 121 RKEAENGDCLQGFQVCHSLGGGTGSGMGTLLISKIREEYPDRMMLTFSVFPSPKVSDTVV 180

Query: 181 EPYNATLSVHQLVENADECMVLDNEALYDICFRTLKLTNPSFGDLNHLISTTMSGVTCCL 240
           EPYNATLSVHQLVENADECMVLDNEALYDICFRTLKLTNPSFGDLNHLISTTMSGVTCCL
Sbjct: 181 EPYNATLSVHQLVENADECMVLDNEALYDICFRTLKLTNPSFGDLNHLISTTMSGVTCCL 240

Query: 241 RFPGQLNSDLRKLAVNLIPFPRLHFFMVGFAPLTARGSQQYRALTIPELTQQMWDAKNMM 300
           RFPGQLNSDLRKLAVNLIPFPRLHFFMVGFAPLTARGSQQYRALTIPELTQQMWDAKNMM
Sbjct: 241 RFPGQLNSDLRKLAVNLIPFPRLHFFMVGFAPLTARGSQQYRALTIPELTQQMWDAKNMM 300

Query: 301 CAADPRHGRYLTASALFRGKMSTKEVDEQMINVQNKNSSYFVEWIPNNVKSSVCDIPPTG 360
           CAADPRHGRYLTASALFRGKMSTKEVDEQMINVQNKNSSYFVEWIPNNVKSSVCDIPPTG
Sbjct: 301 CAADPRHGRYLTASALFRGKMSTKEVDEQMINVQNKNSSYFVEWIPNNVKSSVCDIPPTG 360

Query: 361 LSMSSTFMGNSTSIQEMFRRVSEQFTVMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVS 420
           LSMSSTFMGNSTSIQEMFRRVSEQFTVMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVS
Sbjct: 361 LSMSSTFMGNSTSIQEMFRRVSEQFTVMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVS 420

Query: 421 EYQQYQDAVAEDEEEVEEDVL 442
           EYQQYQDAVAEDEEE EEDVL
Sbjct: 421 EYQQYQDAVAEDEEEEEEDVL 441

BLAST of CSPI05G05440 vs. ExPASy TrEMBL
Match: A0A6J1K569 (Tubulin beta chain OS=Cucurbita maxima OX=3661 GN=LOC111490856 PE=3 SV=1)

HSP 1 Score: 891.3 bits (2302), Expect = 1.6e-255
Identity = 431/435 (99.08%), Postives = 434/435 (99.77%), Query Frame = 0

Query: 1   MREILHIQAGQCGNQIGAKFWEVVCDEHGIDPAGNYTGSSSLQLERVNVYYNEASGGRYV 60
           MREILH+QAGQCGNQIGAKFWEVVCDEHGIDP GNYTG+S+LQLERVNVYYNEASGGRYV
Sbjct: 1   MREILHLQAGQCGNQIGAKFWEVVCDEHGIDPTGNYTGTSNLQLERVNVYYNEASGGRYV 60

Query: 61  PRAVLVDLEPGTMDSLRTGPYGNIFRPDNFVFGQNGAGNNWAKGHYTEGAELVDSVLDVV 120
           PRAVLVDLEPGTMDSLRTGPYGNIFRPDNFVFGQNGAGNNWAKGHYTEGAELVDSVLDVV
Sbjct: 61  PRAVLVDLEPGTMDSLRTGPYGNIFRPDNFVFGQNGAGNNWAKGHYTEGAELVDSVLDVV 120

Query: 121 RKEAENCDCLQGFQVCHSLGGGTGSGMGTLLISKIREEYPDRMMLTFSVFPSPKVSDTVV 180
           RKEAENCDCLQGFQVCHSLGGGTGSGMGTLLISKIREEYPDRMMLTFSVFPSPKVSDTVV
Sbjct: 121 RKEAENCDCLQGFQVCHSLGGGTGSGMGTLLISKIREEYPDRMMLTFSVFPSPKVSDTVV 180

Query: 181 EPYNATLSVHQLVENADECMVLDNEALYDICFRTLKLTNPSFGDLNHLISTTMSGVTCCL 240
           EPYNATLSVHQLVENADECMVLDNEALYDICFRTLKLTNPSFGDLNHLISTTMSGVTCCL
Sbjct: 181 EPYNATLSVHQLVENADECMVLDNEALYDICFRTLKLTNPSFGDLNHLISTTMSGVTCCL 240

Query: 241 RFPGQLNSDLRKLAVNLIPFPRLHFFMVGFAPLTARGSQQYRALTIPELTQQMWDAKNMM 300
           RFPGQLNSDLRKLAVNLIPFPRLHFFMVGFAPLTARGSQQYRALTIPELTQQMWDAKNMM
Sbjct: 241 RFPGQLNSDLRKLAVNLIPFPRLHFFMVGFAPLTARGSQQYRALTIPELTQQMWDAKNMM 300

Query: 301 CAADPRHGRYLTASALFRGKMSTKEVDEQMINVQNKNSSYFVEWIPNNVKSSVCDIPPTG 360
           CAADPRHGRYLTASALFRGKMSTKEVDEQMINVQNKNSSYFVEWIPNNVKSSVCDIPPTG
Sbjct: 301 CAADPRHGRYLTASALFRGKMSTKEVDEQMINVQNKNSSYFVEWIPNNVKSSVCDIPPTG 360

Query: 361 LSMSSTFMGNSTSIQEMFRRVSEQFTVMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVS 420
           LSMSSTFMGNSTSIQEMFRRVSEQFTVMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVS
Sbjct: 361 LSMSSTFMGNSTSIQEMFRRVSEQFTVMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVS 420

Query: 421 EYQQYQDAVAEDEEE 436
           EYQQYQDAVAEDEEE
Sbjct: 421 EYQQYQDAVAEDEEE 435

BLAST of CSPI05G05440 vs. NCBI nr
Match: XP_004143909.2 (tubulin beta chain [Cucumis sativus] >KGN50101.1 hypothetical protein Csa_000672 [Cucumis sativus])

HSP 1 Score: 906.0 bits (2340), Expect = 1.3e-259
Identity = 441/441 (100.00%), Postives = 441/441 (100.00%), Query Frame = 0

Query: 1   MREILHIQAGQCGNQIGAKFWEVVCDEHGIDPAGNYTGSSSLQLERVNVYYNEASGGRYV 60
           MREILHIQAGQCGNQIGAKFWEVVCDEHGIDPAGNYTGSSSLQLERVNVYYNEASGGRYV
Sbjct: 1   MREILHIQAGQCGNQIGAKFWEVVCDEHGIDPAGNYTGSSSLQLERVNVYYNEASGGRYV 60

Query: 61  PRAVLVDLEPGTMDSLRTGPYGNIFRPDNFVFGQNGAGNNWAKGHYTEGAELVDSVLDVV 120
           PRAVLVDLEPGTMDSLRTGPYGNIFRPDNFVFGQNGAGNNWAKGHYTEGAELVDSVLDVV
Sbjct: 61  PRAVLVDLEPGTMDSLRTGPYGNIFRPDNFVFGQNGAGNNWAKGHYTEGAELVDSVLDVV 120

Query: 121 RKEAENCDCLQGFQVCHSLGGGTGSGMGTLLISKIREEYPDRMMLTFSVFPSPKVSDTVV 180
           RKEAENCDCLQGFQVCHSLGGGTGSGMGTLLISKIREEYPDRMMLTFSVFPSPKVSDTVV
Sbjct: 121 RKEAENCDCLQGFQVCHSLGGGTGSGMGTLLISKIREEYPDRMMLTFSVFPSPKVSDTVV 180

Query: 181 EPYNATLSVHQLVENADECMVLDNEALYDICFRTLKLTNPSFGDLNHLISTTMSGVTCCL 240
           EPYNATLSVHQLVENADECMVLDNEALYDICFRTLKLTNPSFGDLNHLISTTMSGVTCCL
Sbjct: 181 EPYNATLSVHQLVENADECMVLDNEALYDICFRTLKLTNPSFGDLNHLISTTMSGVTCCL 240

Query: 241 RFPGQLNSDLRKLAVNLIPFPRLHFFMVGFAPLTARGSQQYRALTIPELTQQMWDAKNMM 300
           RFPGQLNSDLRKLAVNLIPFPRLHFFMVGFAPLTARGSQQYRALTIPELTQQMWDAKNMM
Sbjct: 241 RFPGQLNSDLRKLAVNLIPFPRLHFFMVGFAPLTARGSQQYRALTIPELTQQMWDAKNMM 300

Query: 301 CAADPRHGRYLTASALFRGKMSTKEVDEQMINVQNKNSSYFVEWIPNNVKSSVCDIPPTG 360
           CAADPRHGRYLTASALFRGKMSTKEVDEQMINVQNKNSSYFVEWIPNNVKSSVCDIPPTG
Sbjct: 301 CAADPRHGRYLTASALFRGKMSTKEVDEQMINVQNKNSSYFVEWIPNNVKSSVCDIPPTG 360

Query: 361 LSMSSTFMGNSTSIQEMFRRVSEQFTVMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVS 420
           LSMSSTFMGNSTSIQEMFRRVSEQFTVMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVS
Sbjct: 361 LSMSSTFMGNSTSIQEMFRRVSEQFTVMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVS 420

Query: 421 EYQQYQDAVAEDEEEVEEDVL 442
           EYQQYQDAVAEDEEEVEEDVL
Sbjct: 421 EYQQYQDAVAEDEEEVEEDVL 441

BLAST of CSPI05G05440 vs. NCBI nr
Match: XP_008437269.1 (PREDICTED: tubulin beta chain-like [Cucumis melo] >KAA0042760.1 tubulin beta chain-like [Cucumis melo var. makuwa])

HSP 1 Score: 903.3 bits (2333), Expect = 8.4e-259
Identity = 440/441 (99.77%), Postives = 440/441 (99.77%), Query Frame = 0

Query: 1   MREILHIQAGQCGNQIGAKFWEVVCDEHGIDPAGNYTGSSSLQLERVNVYYNEASGGRYV 60
           MREILHIQAGQCGNQIGAKFWEVVCDEHGIDPAGNYTGSSSLQLERVNVYYNEASGGRYV
Sbjct: 1   MREILHIQAGQCGNQIGAKFWEVVCDEHGIDPAGNYTGSSSLQLERVNVYYNEASGGRYV 60

Query: 61  PRAVLVDLEPGTMDSLRTGPYGNIFRPDNFVFGQNGAGNNWAKGHYTEGAELVDSVLDVV 120
           PRAVLVDLEPGTMDSLRTGPYGNIFRPDNFVFGQNGAGNNWAKGHYTEGAELVDSVLDVV
Sbjct: 61  PRAVLVDLEPGTMDSLRTGPYGNIFRPDNFVFGQNGAGNNWAKGHYTEGAELVDSVLDVV 120

Query: 121 RKEAENCDCLQGFQVCHSLGGGTGSGMGTLLISKIREEYPDRMMLTFSVFPSPKVSDTVV 180
           RKEAENCDCLQGFQVCHSLGGGTGSGMGTLLISKIREEYPDRMMLTFSVFPSPKVSDTVV
Sbjct: 121 RKEAENCDCLQGFQVCHSLGGGTGSGMGTLLISKIREEYPDRMMLTFSVFPSPKVSDTVV 180

Query: 181 EPYNATLSVHQLVENADECMVLDNEALYDICFRTLKLTNPSFGDLNHLISTTMSGVTCCL 240
           EPYNATLSVHQLVENADECMVLDNEALYDICFRTLKLTNPSFGDLNHLISTTMSGVTCCL
Sbjct: 181 EPYNATLSVHQLVENADECMVLDNEALYDICFRTLKLTNPSFGDLNHLISTTMSGVTCCL 240

Query: 241 RFPGQLNSDLRKLAVNLIPFPRLHFFMVGFAPLTARGSQQYRALTIPELTQQMWDAKNMM 300
           RFPGQLNSDLRKLAVNLIPFPRLHFFMVGFAPLTARGSQQYRALTIPELTQQMWDAKNMM
Sbjct: 241 RFPGQLNSDLRKLAVNLIPFPRLHFFMVGFAPLTARGSQQYRALTIPELTQQMWDAKNMM 300

Query: 301 CAADPRHGRYLTASALFRGKMSTKEVDEQMINVQNKNSSYFVEWIPNNVKSSVCDIPPTG 360
           CAADPRHGRYLTASALFRGKMSTKEVDEQMINVQNKNSSYFVEWIPNNVKSSVCDIPPTG
Sbjct: 301 CAADPRHGRYLTASALFRGKMSTKEVDEQMINVQNKNSSYFVEWIPNNVKSSVCDIPPTG 360

Query: 361 LSMSSTFMGNSTSIQEMFRRVSEQFTVMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVS 420
           LSMSSTFMGNSTSIQEMFRRVSEQFTVMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVS
Sbjct: 361 LSMSSTFMGNSTSIQEMFRRVSEQFTVMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVS 420

Query: 421 EYQQYQDAVAEDEEEVEEDVL 442
           EYQQYQDAVAEDEEE EEDVL
Sbjct: 421 EYQQYQDAVAEDEEEGEEDVL 441

BLAST of CSPI05G05440 vs. NCBI nr
Match: XP_038875501.1 (tubulin beta chain-like [Benincasa hispida])

HSP 1 Score: 896.0 bits (2314), Expect = 1.3e-256
Identity = 437/441 (99.09%), Postives = 439/441 (99.55%), Query Frame = 0

Query: 1   MREILHIQAGQCGNQIGAKFWEVVCDEHGIDPAGNYTGSSSLQLERVNVYYNEASGGRYV 60
           MREILHIQAGQCGNQIGAKFWEVVCDEHGIDP GNYTGSS+LQLERVNVYYNEASGGRYV
Sbjct: 1   MREILHIQAGQCGNQIGAKFWEVVCDEHGIDPTGNYTGSSNLQLERVNVYYNEASGGRYV 60

Query: 61  PRAVLVDLEPGTMDSLRTGPYGNIFRPDNFVFGQNGAGNNWAKGHYTEGAELVDSVLDVV 120
           PRAVLVDLEPGTMDSLRTGPYGNIFRPDNFVFGQNGAGNNWAKGHYTEGAELVDSVLDVV
Sbjct: 61  PRAVLVDLEPGTMDSLRTGPYGNIFRPDNFVFGQNGAGNNWAKGHYTEGAELVDSVLDVV 120

Query: 121 RKEAENCDCLQGFQVCHSLGGGTGSGMGTLLISKIREEYPDRMMLTFSVFPSPKVSDTVV 180
           RKEAENCDCLQGFQVCHSLGGGTGSGMGTLLISKIREEYPDRMMLTFSVFPSPKVSDTVV
Sbjct: 121 RKEAENCDCLQGFQVCHSLGGGTGSGMGTLLISKIREEYPDRMMLTFSVFPSPKVSDTVV 180

Query: 181 EPYNATLSVHQLVENADECMVLDNEALYDICFRTLKLTNPSFGDLNHLISTTMSGVTCCL 240
           EPYNATLSVHQLVENADECMVLDNEALYDICFRTLKLTNPSFGDLNHLISTTMSGVTCCL
Sbjct: 181 EPYNATLSVHQLVENADECMVLDNEALYDICFRTLKLTNPSFGDLNHLISTTMSGVTCCL 240

Query: 241 RFPGQLNSDLRKLAVNLIPFPRLHFFMVGFAPLTARGSQQYRALTIPELTQQMWDAKNMM 300
           RFPGQLNSDLRKLAVNLIPFPRLHFFMVGFAPLTARGSQQYRALTIPELTQQMWDAKNMM
Sbjct: 241 RFPGQLNSDLRKLAVNLIPFPRLHFFMVGFAPLTARGSQQYRALTIPELTQQMWDAKNMM 300

Query: 301 CAADPRHGRYLTASALFRGKMSTKEVDEQMINVQNKNSSYFVEWIPNNVKSSVCDIPPTG 360
           CAADPRHGRYLTASALFRGKMSTKEVDEQMINVQNKNSSYFVEWIPNNVKSSVCDIPPTG
Sbjct: 301 CAADPRHGRYLTASALFRGKMSTKEVDEQMINVQNKNSSYFVEWIPNNVKSSVCDIPPTG 360

Query: 361 LSMSSTFMGNSTSIQEMFRRVSEQFTVMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVS 420
           LSMSSTFMGNSTSIQEMFRRVSEQFTVMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVS
Sbjct: 361 LSMSSTFMGNSTSIQEMFRRVSEQFTVMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVS 420

Query: 421 EYQQYQDAVAEDEEEVEEDVL 442
           EYQQYQDAVAEDEEE EED+L
Sbjct: 421 EYQQYQDAVAEDEEE-EEDML 440

BLAST of CSPI05G05440 vs. NCBI nr
Match: XP_022159568.1 (tubulin beta chain-like [Momordica charantia])

HSP 1 Score: 894.4 bits (2310), Expect = 3.9e-256
Identity = 435/441 (98.64%), Postives = 437/441 (99.09%), Query Frame = 0

Query: 1   MREILHIQAGQCGNQIGAKFWEVVCDEHGIDPAGNYTGSSSLQLERVNVYYNEASGGRYV 60
           MREILH+QAGQCGNQIGAKFWEVVCDEHGIDP GNY GSSS+QLERVNVYYNEASGGRYV
Sbjct: 1   MREILHVQAGQCGNQIGAKFWEVVCDEHGIDPTGNYVGSSSIQLERVNVYYNEASGGRYV 60

Query: 61  PRAVLVDLEPGTMDSLRTGPYGNIFRPDNFVFGQNGAGNNWAKGHYTEGAELVDSVLDVV 120
           PRAVLVDLEPGTMDSLRTGPYGNIFRPDNFVFGQNGAGNNWAKGHYTEGAELVDSVLDVV
Sbjct: 61  PRAVLVDLEPGTMDSLRTGPYGNIFRPDNFVFGQNGAGNNWAKGHYTEGAELVDSVLDVV 120

Query: 121 RKEAENCDCLQGFQVCHSLGGGTGSGMGTLLISKIREEYPDRMMLTFSVFPSPKVSDTVV 180
           RKEAEN DCLQGFQVCHSLGGGTGSGMGTLLISKIREEYPDRMMLTFSVFPSPKVSDTVV
Sbjct: 121 RKEAENGDCLQGFQVCHSLGGGTGSGMGTLLISKIREEYPDRMMLTFSVFPSPKVSDTVV 180

Query: 181 EPYNATLSVHQLVENADECMVLDNEALYDICFRTLKLTNPSFGDLNHLISTTMSGVTCCL 240
           EPYNATLSVHQLVENADECMVLDNEALYDICFRTLKLTNPSFGDLNHLISTTMSGVTCCL
Sbjct: 181 EPYNATLSVHQLVENADECMVLDNEALYDICFRTLKLTNPSFGDLNHLISTTMSGVTCCL 240

Query: 241 RFPGQLNSDLRKLAVNLIPFPRLHFFMVGFAPLTARGSQQYRALTIPELTQQMWDAKNMM 300
           RFPGQLNSDLRKLAVNLIPFPRLHFFMVGFAPLTARGSQQYRALTIPELTQQMWDAKNMM
Sbjct: 241 RFPGQLNSDLRKLAVNLIPFPRLHFFMVGFAPLTARGSQQYRALTIPELTQQMWDAKNMM 300

Query: 301 CAADPRHGRYLTASALFRGKMSTKEVDEQMINVQNKNSSYFVEWIPNNVKSSVCDIPPTG 360
           CAADPRHGRYLTASALFRGKMSTKEVDEQMINVQNKNSSYFVEWIPNNVKSSVCDIPPTG
Sbjct: 301 CAADPRHGRYLTASALFRGKMSTKEVDEQMINVQNKNSSYFVEWIPNNVKSSVCDIPPTG 360

Query: 361 LSMSSTFMGNSTSIQEMFRRVSEQFTVMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVS 420
           LSMSSTFMGNSTSIQEMFRRVSEQFTVMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVS
Sbjct: 361 LSMSSTFMGNSTSIQEMFRRVSEQFTVMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVS 420

Query: 421 EYQQYQDAVAEDEEEVEEDVL 442
           EYQQYQDAVAEDEEE EEDVL
Sbjct: 421 EYQQYQDAVAEDEEEEEEDVL 441

BLAST of CSPI05G05440 vs. NCBI nr
Match: XP_022958255.1 (tubulin beta chain-like [Cucurbita moschata] >XP_022995254.1 tubulin beta chain-like [Cucurbita maxima] >XP_023533713.1 tubulin beta chain-like [Cucurbita pepo subsp. pepo] >KAG6606097.1 Tubulin beta-1 chain, partial [Cucurbita argyrosperma subsp. sororia] >KAG7036043.1 Tubulin beta-1 chain [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 891.3 bits (2302), Expect = 3.3e-255
Identity = 431/435 (99.08%), Postives = 434/435 (99.77%), Query Frame = 0

Query: 1   MREILHIQAGQCGNQIGAKFWEVVCDEHGIDPAGNYTGSSSLQLERVNVYYNEASGGRYV 60
           MREILH+QAGQCGNQIGAKFWEVVCDEHGIDP GNYTG+S+LQLERVNVYYNEASGGRYV
Sbjct: 1   MREILHLQAGQCGNQIGAKFWEVVCDEHGIDPTGNYTGTSNLQLERVNVYYNEASGGRYV 60

Query: 61  PRAVLVDLEPGTMDSLRTGPYGNIFRPDNFVFGQNGAGNNWAKGHYTEGAELVDSVLDVV 120
           PRAVLVDLEPGTMDSLRTGPYGNIFRPDNFVFGQNGAGNNWAKGHYTEGAELVDSVLDVV
Sbjct: 61  PRAVLVDLEPGTMDSLRTGPYGNIFRPDNFVFGQNGAGNNWAKGHYTEGAELVDSVLDVV 120

Query: 121 RKEAENCDCLQGFQVCHSLGGGTGSGMGTLLISKIREEYPDRMMLTFSVFPSPKVSDTVV 180
           RKEAENCDCLQGFQVCHSLGGGTGSGMGTLLISKIREEYPDRMMLTFSVFPSPKVSDTVV
Sbjct: 121 RKEAENCDCLQGFQVCHSLGGGTGSGMGTLLISKIREEYPDRMMLTFSVFPSPKVSDTVV 180

Query: 181 EPYNATLSVHQLVENADECMVLDNEALYDICFRTLKLTNPSFGDLNHLISTTMSGVTCCL 240
           EPYNATLSVHQLVENADECMVLDNEALYDICFRTLKLTNPSFGDLNHLISTTMSGVTCCL
Sbjct: 181 EPYNATLSVHQLVENADECMVLDNEALYDICFRTLKLTNPSFGDLNHLISTTMSGVTCCL 240

Query: 241 RFPGQLNSDLRKLAVNLIPFPRLHFFMVGFAPLTARGSQQYRALTIPELTQQMWDAKNMM 300
           RFPGQLNSDLRKLAVNLIPFPRLHFFMVGFAPLTARGSQQYRALTIPELTQQMWDAKNMM
Sbjct: 241 RFPGQLNSDLRKLAVNLIPFPRLHFFMVGFAPLTARGSQQYRALTIPELTQQMWDAKNMM 300

Query: 301 CAADPRHGRYLTASALFRGKMSTKEVDEQMINVQNKNSSYFVEWIPNNVKSSVCDIPPTG 360
           CAADPRHGRYLTASALFRGKMSTKEVDEQMINVQNKNSSYFVEWIPNNVKSSVCDIPPTG
Sbjct: 301 CAADPRHGRYLTASALFRGKMSTKEVDEQMINVQNKNSSYFVEWIPNNVKSSVCDIPPTG 360

Query: 361 LSMSSTFMGNSTSIQEMFRRVSEQFTVMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVS 420
           LSMSSTFMGNSTSIQEMFRRVSEQFTVMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVS
Sbjct: 361 LSMSSTFMGNSTSIQEMFRRVSEQFTVMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVS 420

Query: 421 EYQQYQDAVAEDEEE 436
           EYQQYQDAVAEDEEE
Sbjct: 421 EYQQYQDAVAEDEEE 435

BLAST of CSPI05G05440 vs. TAIR 10
Match: AT5G12250.1 (beta-6 tubulin )

HSP 1 Score: 854.7 bits (2207), Expect = 3.2e-248
Identity = 409/439 (93.17%), Postives = 424/439 (96.58%), Query Frame = 0

Query: 1   MREILHIQAGQCGNQIGAKFWEVVCDEHGIDPAGNYTGSSSLQLERVNVYYNEASGGRYV 60
           MREILHIQ GQCGNQIG+KFWEVVCDEHGIDP G Y G+S LQLERVNVYYNEAS GRYV
Sbjct: 1   MREILHIQGGQCGNQIGSKFWEVVCDEHGIDPTGRYVGNSDLQLERVNVYYNEASCGRYV 60

Query: 61  PRAVLVDLEPGTMDSLRTGPYGNIFRPDNFVFGQNGAGNNWAKGHYTEGAELVDSVLDVV 120
           PRA+L+DLEPGTMDS+RTGPYG IFRPDNFVFGQ+GAGNNWAKGHYTEGAEL+D+VLDVV
Sbjct: 61  PRAILMDLEPGTMDSVRTGPYGQIFRPDNFVFGQSGAGNNWAKGHYTEGAELIDAVLDVV 120

Query: 121 RKEAENCDCLQGFQVCHSLGGGTGSGMGTLLISKIREEYPDRMMLTFSVFPSPKVSDTVV 180
           RKEAENCDCLQGFQVCHSLGGGTGSGMGTLLISKIREEYPDRMMLTFSVFPSPKVSDTVV
Sbjct: 121 RKEAENCDCLQGFQVCHSLGGGTGSGMGTLLISKIREEYPDRMMLTFSVFPSPKVSDTVV 180

Query: 181 EPYNATLSVHQLVENADECMVLDNEALYDICFRTLKLTNPSFGDLNHLISTTMSGVTCCL 240
           EPYNATLSVHQLVENADECMVLDNEALYDICFRTLKLT PSFGDLNHLIS TMSGVTCCL
Sbjct: 181 EPYNATLSVHQLVENADECMVLDNEALYDICFRTLKLTTPSFGDLNHLISATMSGVTCCL 240

Query: 241 RFPGQLNSDLRKLAVNLIPFPRLHFFMVGFAPLTARGSQQYRALTIPELTQQMWDAKNMM 300
           RFPGQLNSDLRKLAVNLIPFPRLHFFMVGFAPLT+RGSQQYRALT+PELTQQMWD+KNMM
Sbjct: 241 RFPGQLNSDLRKLAVNLIPFPRLHFFMVGFAPLTSRGSQQYRALTVPELTQQMWDSKNMM 300

Query: 301 CAADPRHGRYLTASALFRGKMSTKEVDEQMINVQNKNSSYFVEWIPNNVKSSVCDIPPTG 360
           CAADPRHGRYLTASA+FRGKMSTKEVDEQMINVQNKNSSYFVEWIPNNVKSSVCDI P G
Sbjct: 301 CAADPRHGRYLTASAMFRGKMSTKEVDEQMINVQNKNSSYFVEWIPNNVKSSVCDIAPRG 360

Query: 361 LSMSSTFMGNSTSIQEMFRRVSEQFTVMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVS 420
           LSM+STF+GNSTSIQEMFRRVSEQFT MFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVS
Sbjct: 361 LSMASTFIGNSTSIQEMFRRVSEQFTAMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVS 420

Query: 421 EYQQYQDAVAEDEEEVEED 440
           EYQQYQDA A+DE E EED
Sbjct: 421 EYQQYQDATADDEGEYEED 439

BLAST of CSPI05G05440 vs. TAIR 10
Match: AT5G62690.1 (tubulin beta chain 2 )

HSP 1 Score: 847.0 bits (2187), Expect = 6.6e-246
Identity = 403/439 (91.80%), Postives = 423/439 (96.36%), Query Frame = 0

Query: 1   MREILHIQAGQCGNQIGAKFWEVVCDEHGIDPAGNYTGSSSLQLERVNVYYNEASGGRYV 60
           MREILHIQ GQCGNQIGAKFWEVVC EHGIDP G YTG S LQLER+NVYYNEAS GR+V
Sbjct: 1   MREILHIQGGQCGNQIGAKFWEVVCAEHGIDPTGRYTGDSDLQLERINVYYNEASCGRFV 60

Query: 61  PRAVLVDLEPGTMDSLRTGPYGNIFRPDNFVFGQNGAGNNWAKGHYTEGAELVDSVLDVV 120
           PRAVL+DLEPGTMDSLR+GPYG  FRPDNFVFGQ+GAGNNWAKGHYTEGAEL+DSVLDVV
Sbjct: 61  PRAVLMDLEPGTMDSLRSGPYGQTFRPDNFVFGQSGAGNNWAKGHYTEGAELIDSVLDVV 120

Query: 121 RKEAENCDCLQGFQVCHSLGGGTGSGMGTLLISKIREEYPDRMMLTFSVFPSPKVSDTVV 180
           RKEAENCDCLQGFQVCHSLGGGTGSGMGTLLISKIREEYPDRMMLTFSVFPSPKVSDTVV
Sbjct: 121 RKEAENCDCLQGFQVCHSLGGGTGSGMGTLLISKIREEYPDRMMLTFSVFPSPKVSDTVV 180

Query: 181 EPYNATLSVHQLVENADECMVLDNEALYDICFRTLKLTNPSFGDLNHLISTTMSGVTCCL 240
           EPYNATLSVHQLVENADECMVLDNEALYDICFRTLKLT PSFGDLNHLIS TMSGVTCCL
Sbjct: 181 EPYNATLSVHQLVENADECMVLDNEALYDICFRTLKLTTPSFGDLNHLISATMSGVTCCL 240

Query: 241 RFPGQLNSDLRKLAVNLIPFPRLHFFMVGFAPLTARGSQQYRALTIPELTQQMWDAKNMM 300
           RFPGQLNSDLRKLAVNLIPFPRLHFFMVGFAPLT+RGSQQYR+LT+PELTQQMWD+KNMM
Sbjct: 241 RFPGQLNSDLRKLAVNLIPFPRLHFFMVGFAPLTSRGSQQYRSLTVPELTQQMWDSKNMM 300

Query: 301 CAADPRHGRYLTASALFRGKMSTKEVDEQMINVQNKNSSYFVEWIPNNVKSSVCDIPPTG 360
           CAADPRHGRYLTASA+FRGKMSTKEVDEQM+NVQNKNSSYFVEWIPNNVKS+VCDIPPTG
Sbjct: 301 CAADPRHGRYLTASAMFRGKMSTKEVDEQMLNVQNKNSSYFVEWIPNNVKSTVCDIPPTG 360

Query: 361 LSMSSTFMGNSTSIQEMFRRVSEQFTVMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVS 420
           L M+STF+GNSTSIQEMFRRVSEQFT MFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVS
Sbjct: 361 LKMASTFIGNSTSIQEMFRRVSEQFTAMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVS 420

Query: 421 EYQQYQDAVAEDEEEVEED 440
           EYQQYQDA A++E + E++
Sbjct: 421 EYQQYQDATADEEGDYEDE 439

BLAST of CSPI05G05440 vs. TAIR 10
Match: AT5G62700.1 (tubulin beta chain 3 )

HSP 1 Score: 847.0 bits (2187), Expect = 6.6e-246
Identity = 403/439 (91.80%), Postives = 423/439 (96.36%), Query Frame = 0

Query: 1   MREILHIQAGQCGNQIGAKFWEVVCDEHGIDPAGNYTGSSSLQLERVNVYYNEASGGRYV 60
           MREILHIQ GQCGNQIGAKFWEVVC EHGIDP G YTG S LQLER+NVYYNEAS GR+V
Sbjct: 1   MREILHIQGGQCGNQIGAKFWEVVCAEHGIDPTGRYTGDSDLQLERINVYYNEASCGRFV 60

Query: 61  PRAVLVDLEPGTMDSLRTGPYGNIFRPDNFVFGQNGAGNNWAKGHYTEGAELVDSVLDVV 120
           PRAVL+DLEPGTMDSLR+GPYG  FRPDNFVFGQ+GAGNNWAKGHYTEGAEL+DSVLDVV
Sbjct: 61  PRAVLMDLEPGTMDSLRSGPYGQTFRPDNFVFGQSGAGNNWAKGHYTEGAELIDSVLDVV 120

Query: 121 RKEAENCDCLQGFQVCHSLGGGTGSGMGTLLISKIREEYPDRMMLTFSVFPSPKVSDTVV 180
           RKEAENCDCLQGFQVCHSLGGGTGSGMGTLLISKIREEYPDRMMLTFSVFPSPKVSDTVV
Sbjct: 121 RKEAENCDCLQGFQVCHSLGGGTGSGMGTLLISKIREEYPDRMMLTFSVFPSPKVSDTVV 180

Query: 181 EPYNATLSVHQLVENADECMVLDNEALYDICFRTLKLTNPSFGDLNHLISTTMSGVTCCL 240
           EPYNATLSVHQLVENADECMVLDNEALYDICFRTLKLT PSFGDLNHLIS TMSGVTCCL
Sbjct: 181 EPYNATLSVHQLVENADECMVLDNEALYDICFRTLKLTTPSFGDLNHLISATMSGVTCCL 240

Query: 241 RFPGQLNSDLRKLAVNLIPFPRLHFFMVGFAPLTARGSQQYRALTIPELTQQMWDAKNMM 300
           RFPGQLNSDLRKLAVNLIPFPRLHFFMVGFAPLT+RGSQQYR+LT+PELTQQMWD+KNMM
Sbjct: 241 RFPGQLNSDLRKLAVNLIPFPRLHFFMVGFAPLTSRGSQQYRSLTVPELTQQMWDSKNMM 300

Query: 301 CAADPRHGRYLTASALFRGKMSTKEVDEQMINVQNKNSSYFVEWIPNNVKSSVCDIPPTG 360
           CAADPRHGRYLTASA+FRGKMSTKEVDEQM+NVQNKNSSYFVEWIPNNVKS+VCDIPPTG
Sbjct: 301 CAADPRHGRYLTASAMFRGKMSTKEVDEQMLNVQNKNSSYFVEWIPNNVKSTVCDIPPTG 360

Query: 361 LSMSSTFMGNSTSIQEMFRRVSEQFTVMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVS 420
           L M+STF+GNSTSIQEMFRRVSEQFT MFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVS
Sbjct: 361 LKMASTFIGNSTSIQEMFRRVSEQFTAMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVS 420

Query: 421 EYQQYQDAVAEDEEEVEED 440
           EYQQYQDA A++E + E++
Sbjct: 421 EYQQYQDATADEEGDYEDE 439

BLAST of CSPI05G05440 vs. TAIR 10
Match: AT5G23860.1 (tubulin beta 8 )

HSP 1 Score: 846.3 bits (2185), Expect = 1.1e-245
Identity = 408/441 (92.52%), Postives = 423/441 (95.92%), Query Frame = 0

Query: 1   MREILHIQAGQCGNQIGAKFWEVVCDEHGIDPAGNYTGSSSLQLERVNVYYNEASGGRYV 60
           MREILHIQ GQCGNQIGAKFWEVVC EHGID  G Y G + LQLERVNVYYNEAS GR+V
Sbjct: 1   MREILHIQGGQCGNQIGAKFWEVVCAEHGIDSTGRYQGENDLQLERVNVYYNEASCGRFV 60

Query: 61  PRAVLVDLEPGTMDSLRTGPYGNIFRPDNFVFGQNGAGNNWAKGHYTEGAELVDSVLDVV 120
           PRAVL+DLEPGTMDS+R+GPYG IFRPDNFVFGQ+GAGNNWAKGHYTEGAEL+DSVLDVV
Sbjct: 61  PRAVLMDLEPGTMDSVRSGPYGQIFRPDNFVFGQSGAGNNWAKGHYTEGAELIDSVLDVV 120

Query: 121 RKEAENCDCLQGFQVCHSLGGGTGSGMGTLLISKIREEYPDRMMLTFSVFPSPKVSDTVV 180
           RKEAENCDCLQGFQVCHSLGGGTGSGMGTLLISKIREEYPDRMMLTFSVFPSPKVSDTVV
Sbjct: 121 RKEAENCDCLQGFQVCHSLGGGTGSGMGTLLISKIREEYPDRMMLTFSVFPSPKVSDTVV 180

Query: 181 EPYNATLSVHQLVENADECMVLDNEALYDICFRTLKLTNPSFGDLNHLISTTMSGVTCCL 240
           EPYNATLSVHQLVENADECMVLDNEALYDICFRTLKLT PSFGDLNHLIS TMSGVTCCL
Sbjct: 181 EPYNATLSVHQLVENADECMVLDNEALYDICFRTLKLTTPSFGDLNHLISATMSGVTCCL 240

Query: 241 RFPGQLNSDLRKLAVNLIPFPRLHFFMVGFAPLTARGSQQYRALTIPELTQQMWDAKNMM 300
           RFPGQLNSDLRKLAVNLIPFPRLHFFMVGFAPLT+RGSQQYRALT+PELTQQMWDAKNMM
Sbjct: 241 RFPGQLNSDLRKLAVNLIPFPRLHFFMVGFAPLTSRGSQQYRALTVPELTQQMWDAKNMM 300

Query: 301 CAADPRHGRYLTASALFRGKMSTKEVDEQMINVQNKNSSYFVEWIPNNVKSSVCDIPPTG 360
           CAADPRHGRYLTASA+FRGKMSTKEVDEQMINVQNKNSSYFVEWIPNNVKS+VCDIPPTG
Sbjct: 301 CAADPRHGRYLTASAMFRGKMSTKEVDEQMINVQNKNSSYFVEWIPNNVKSTVCDIPPTG 360

Query: 361 LSMSSTFMGNSTSIQEMFRRVSEQFTVMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVS 420
           L M+STF+GNSTSIQEMFRRVSEQFT MFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVS
Sbjct: 361 LKMASTFIGNSTSIQEMFRRVSEQFTAMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVS 420

Query: 421 EYQQYQDAVAEDEE--EVEED 440
           EYQQYQDA A++EE  E EED
Sbjct: 421 EYQQYQDATADEEEGYEYEED 441

BLAST of CSPI05G05440 vs. TAIR 10
Match: AT5G23860.2 (tubulin beta 8 )

HSP 1 Score: 846.3 bits (2185), Expect = 1.1e-245
Identity = 408/441 (92.52%), Postives = 423/441 (95.92%), Query Frame = 0

Query: 1   MREILHIQAGQCGNQIGAKFWEVVCDEHGIDPAGNYTGSSSLQLERVNVYYNEASGGRYV 60
           MREILHIQ GQCGNQIGAKFWEVVC EHGID  G Y G + LQLERVNVYYNEAS GR+V
Sbjct: 1   MREILHIQGGQCGNQIGAKFWEVVCAEHGIDSTGRYQGENDLQLERVNVYYNEASCGRFV 60

Query: 61  PRAVLVDLEPGTMDSLRTGPYGNIFRPDNFVFGQNGAGNNWAKGHYTEGAELVDSVLDVV 120
           PRAVL+DLEPGTMDS+R+GPYG IFRPDNFVFGQ+GAGNNWAKGHYTEGAEL+DSVLDVV
Sbjct: 61  PRAVLMDLEPGTMDSVRSGPYGQIFRPDNFVFGQSGAGNNWAKGHYTEGAELIDSVLDVV 120

Query: 121 RKEAENCDCLQGFQVCHSLGGGTGSGMGTLLISKIREEYPDRMMLTFSVFPSPKVSDTVV 180
           RKEAENCDCLQGFQVCHSLGGGTGSGMGTLLISKIREEYPDRMMLTFSVFPSPKVSDTVV
Sbjct: 121 RKEAENCDCLQGFQVCHSLGGGTGSGMGTLLISKIREEYPDRMMLTFSVFPSPKVSDTVV 180

Query: 181 EPYNATLSVHQLVENADECMVLDNEALYDICFRTLKLTNPSFGDLNHLISTTMSGVTCCL 240
           EPYNATLSVHQLVENADECMVLDNEALYDICFRTLKLT PSFGDLNHLIS TMSGVTCCL
Sbjct: 181 EPYNATLSVHQLVENADECMVLDNEALYDICFRTLKLTTPSFGDLNHLISATMSGVTCCL 240

Query: 241 RFPGQLNSDLRKLAVNLIPFPRLHFFMVGFAPLTARGSQQYRALTIPELTQQMWDAKNMM 300
           RFPGQLNSDLRKLAVNLIPFPRLHFFMVGFAPLT+RGSQQYRALT+PELTQQMWDAKNMM
Sbjct: 241 RFPGQLNSDLRKLAVNLIPFPRLHFFMVGFAPLTSRGSQQYRALTVPELTQQMWDAKNMM 300

Query: 301 CAADPRHGRYLTASALFRGKMSTKEVDEQMINVQNKNSSYFVEWIPNNVKSSVCDIPPTG 360
           CAADPRHGRYLTASA+FRGKMSTKEVDEQMINVQNKNSSYFVEWIPNNVKS+VCDIPPTG
Sbjct: 301 CAADPRHGRYLTASAMFRGKMSTKEVDEQMINVQNKNSSYFVEWIPNNVKSTVCDIPPTG 360

Query: 361 LSMSSTFMGNSTSIQEMFRRVSEQFTVMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVS 420
           L M+STF+GNSTSIQEMFRRVSEQFT MFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVS
Sbjct: 361 LKMASTFIGNSTSIQEMFRRVSEQFTAMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVS 420

Query: 421 EYQQYQDAVAEDEE--EVEED 440
           EYQQYQDA A++EE  E EED
Sbjct: 421 EYQQYQDATADEEEGYEYEED 441

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
P931761.1e-24893.85Tubulin beta chain OS=Hordeum vulgare OX=4513 GN=TUBB PE=2 SV=1[more]
Q9ZRB14.1e-24893.62Tubulin beta-2 chain OS=Triticum aestivum OX=4565 GN=TUBB2 PE=2 SV=1[more]
Q417834.1e-24893.47Tubulin beta-6 chain OS=Zea mays OX=4577 GN=TUBB6 PE=2 SV=1[more]
P459605.3e-24893.62Tubulin beta-4 chain OS=Oryza sativa subsp. japonica OX=39947 GN=TUBB4 PE=2 SV=1[more]
Q9ZPN99.0e-24893.18Tubulin beta-2 chain OS=Eleusine indica OX=29674 GN=TUBB2 PE=2 SV=1[more]
Match NameE-valueIdentityDescription
A0A0A0KQW76.3e-260100.00Tubulin beta chain OS=Cucumis sativus OX=3659 GN=Csa_5G153160 PE=3 SV=1[more]
A0A5A7THJ14.1e-25999.77Tubulin beta chain OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold44G00... [more]
A0A1S3ATL64.1e-25999.77Tubulin beta chain OS=Cucumis melo OX=3656 GN=LOC103482745 PE=3 SV=1[more]
A0A6J1DZ611.9e-25698.64Tubulin beta chain OS=Momordica charantia OX=3673 GN=LOC111025947 PE=3 SV=1[more]
A0A6J1K5691.6e-25599.08Tubulin beta chain OS=Cucurbita maxima OX=3661 GN=LOC111490856 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
XP_004143909.21.3e-259100.00tubulin beta chain [Cucumis sativus] >KGN50101.1 hypothetical protein Csa_000672... [more]
XP_008437269.18.4e-25999.77PREDICTED: tubulin beta chain-like [Cucumis melo] >KAA0042760.1 tubulin beta cha... [more]
XP_038875501.11.3e-25699.09tubulin beta chain-like [Benincasa hispida][more]
XP_022159568.13.9e-25698.64tubulin beta chain-like [Momordica charantia][more]
XP_022958255.13.3e-25599.08tubulin beta chain-like [Cucurbita moschata] >XP_022995254.1 tubulin beta chain-... [more]
Match NameE-valueIdentityDescription
AT5G12250.13.2e-24893.17beta-6 tubulin [more]
AT5G62690.16.6e-24691.80tubulin beta chain 2 [more]
AT5G62700.16.6e-24691.80tubulin beta chain 3 [more]
AT5G23860.11.1e-24592.52tubulin beta 8 [more]
AT5G23860.21.1e-24592.52tubulin beta 8 [more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (PI 183967) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 408..435
NoneNo IPR availablePANTHERPTHR11588:SF396TUBULIN BETA CHAINcoord: 1..436
NoneNo IPR availableCDDcd02187beta_tubulincoord: 2..426
e-value: 0.0
score: 871.503
IPR000217TubulinPRINTSPR01161TUBULINcoord: 190..210
score: 67.82
coord: 10..30
score: 72.97
coord: 132..150
score: 79.04
coord: 370..398
score: 63.78
coord: 51..70
score: 69.96
coord: 93..104
score: 72.69
coord: 106..130
score: 70.76
coord: 151..172
score: 59.63
coord: 176..189
score: 79.11
IPR000217TubulinPANTHERPTHR11588TUBULINcoord: 1..436
IPR002453Beta tubulinPRINTSPR01163BETATUBULINcoord: 265..281
score: 82.86
coord: 311..329
score: 78.83
coord: 41..58
score: 74.89
coord: 347..370
score: 73.48
coord: 108..126
score: 88.12
coord: 230..239
score: 75.09
coord: 329..343
score: 87.09
coord: 152..164
score: 91.43
coord: 377..388
score: 71.71
coord: 88..99
score: 87.95
coord: 212..224
score: 77.71
coord: 412..430
score: 89.23
coord: 245..258
score: 88.61
IPR003008Tubulin/FtsZ, GTPase domainSMARTSM00864Tubulin_4coord: 47..244
e-value: 2.5E-69
score: 246.3
IPR003008Tubulin/FtsZ, GTPase domainPFAMPF00091Tubulincoord: 3..211
e-value: 1.2E-69
score: 234.5
IPR018316Tubulin/FtsZ, 2-layer sandwich domainSMARTSM00865Tubulin_C_4coord: 246..383
e-value: 5.5E-40
score: 148.8
IPR018316Tubulin/FtsZ, 2-layer sandwich domainPFAMPF03953Tubulin_Ccoord: 261..382
e-value: 1.6E-38
score: 131.7
IPR036525Tubulin/FtsZ, GTPase domain superfamilyGENE3D3.40.50.1440Tubulin/FtsZ, GTPase domaincoord: 1..262
e-value: 1.0E-131
score: 440.3
IPR036525Tubulin/FtsZ, GTPase domain superfamilySUPERFAMILY52490Tubulin nucleotide-binding domain-likecoord: 1..243
IPR037103Tubulin/FtsZ-like, C-terminal domainGENE3D3.30.1330.20coord: 263..373
e-value: 1.3E-40
score: 140.1
IPR023123Tubulin, C-terminalGENE3D1.10.287.600Helix hairpin bincoord: 374..429
e-value: 4.6E-34
score: 118.0
IPR013838Beta tubulin, autoregulation binding sitePROSITEPS00228TUBULIN_B_AUTOREGcoord: 1..4
IPR017975Tubulin, conserved sitePROSITEPS00227TUBULINcoord: 140..146
IPR008280Tubulin/FtsZ, C-terminalSUPERFAMILY55307Tubulin C-terminal domain-likecoord: 244..428

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CSPI05G05440.1CSPI05G05440.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0000226 microtubule cytoskeleton organization
biological_process GO:0000278 mitotic cell cycle
biological_process GO:0007017 microtubule-based process
cellular_component GO:0005737 cytoplasm
cellular_component GO:0005874 microtubule
molecular_function GO:0003924 GTPase activity
molecular_function GO:0005525 GTP binding
molecular_function GO:0005200 structural constituent of cytoskeleton