CSPI05G02400 (gene) Cucumber (PI 183967) v1

Overview


Name	CSPI05G02400
Type	gene
Organism	Cucumis sativus var. hardwickii cv. PI 183967 (Cucumber (PI 183967) v1)
Description	Pectinesterase
Location	Chr5: 3072676 .. 3074778 (+)
RNA-Seq Expression	CSPI05G02400
Synteny	CSPI05G02400

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGCTCGGGAAGGTGATCGTGTCGGGATTATCCCTTATTCTCGTTGTTGGTATAGCTCTAGCCATTGCCGCCACGATAAACAAAAGCAATGCTTCTAATGGTGGAACAGAGGACGTATCACCAAAGATGAAGGCAGTCTCAGCCATTTGTTCTACTACAGATTATCAAGATGAATGCAAAACTACACTTGACCATGTTGCTCGTAATACCTCTTCAAATGACCCCAAAGACTATGCTGAAGCAGCAATTCTTGCCACTATTGGGGAAATTACAAAAGGCTACAACTTGTCTGATAGCCTTATCGTGGAGGCCAGTACCAATGCCTCCATCAAAATGAGTGTTGAAGATTGCAAGGATTTGTTACAATTCGCCATTGATGAGTTGCAAGCATCGTATTCCGCTGTTGGAGAGTCTGATTTGCATACCGATGCAGATCGTGTTGCTGATATTAAGAATTGGCTTAGTGCTGTTATTTCTTATCAACAATCATGTCTTGATGGACTTGGAGAATTTGATCCTCAGCTTAAACAGAGGATGCAAGATGGTCTTGATGTTGCTGGCAAGCTTACCAGCAATGCTCTTGCTATTGTCACTGCAGTTTCCAATATCCTCGACAACTACCGCTTGCAATTGAAGGTAATTATGCTAAATCATCACTTATTAGAAAGCACTTTTTAACTCCATCTAGTTGGATGGAGTGGAGGAGTTGAGGGTAGTCAAGTCGTAAACTTGGCCTGAACGAATTTGCAGTCCTATTACTAAAAAAAAAATCTCAATTTTATACCTTATTAACTTCTTACATGGTAGAAACCACGAGTTTACAATAACTCCACTCCTTACCTCAAACAACCTCGTAAAAAGTTTAAACCAATGGGATATGACATATTTAATAAGTGTAGTTTGTTTTTTATTAGCAGGTTCAACCAAGTGGCCGTAGGCTTCTTGGAACAACAGTAGTAGATAGAGACGGGTTTCCGACATGGTTGACGGGGGCAGATAGGAAGTTGCTTGCATCGAAACAAGGAGGCGTTCGACCAACACCGAACGCAGTGGTGGCTAAGGACGGTAGTGGGAAATATAAGACCATTGCTGCTGCATTAGCTGCATACCCGAAGGTCCTAAGAGGAAGATACGTAATCTACGTGAAAGCAGGGATCTATGATGAATACATCACTCTAACTAAAGACATGAAAAATGTTTTCATGTATGGAGATGGTCCAAGAAAAACCATTGTCACGGGTCGCAAGAGCAACCGCGACGGTTTCACCACACAAAACACTGCCTCTTTTGGTAAATCACTCCCTACTCCAATTTTTCCCAACAATAATATTTTGTCCTAAATTTTCAAATTATCATAAGATATAATATACAAACTAACACATTTACACTTCGAAATTACCTCTTGCAGCTGCCATAGGGGAAGGATTTCTTTGCAAATCCATGGGATTCACCAACACAGCTGGTCCTGAAGGCCACCAAGCAGTAGCCTTACGCGTTCAATCCGATAGATCCGCCTTCTTCAACTGCAGAATGGACGGTCATCAAGACACTCTCTACGTACAAACTCACCGTCAATTCTACCGCAACTGTGTCGTATCGGGTACCGTAGACTTCATCTTCGGTGATTCATCCACCGTCATCCAAAACTCCTTAATCATCGTTCGCCGCCCAATGGACAACCAACAAAACACCGTCACAGCCCAAGGTCGCGCCGAGCAAAAAGAAATCACCGGTCTAGTGATCCACAACTGCAGAATCGTCCCAGAACAGAAGTTGTTCGCTGAGAGGTTCAAGATTCCAACATTCTTGGGGCGACCATGGAAGCAATATGCGAGAACTGTGATCATGGAGTCTACATTGGGTGATTTCATTCAACCAGCTGGATACATGCCTTGGTCTGGAGATTTTGCATTGGAGACATGTTTGTATTTGGAGTATGGGAACAGAGGACCTGGTGCTAACACCAACAGAAGAGTGAGATGGAAGGGTGCTAAAGTGATTGGAAGAAATGAAGCTTTGCAATATACTGCTGGTGCATTTCTTCTCGGAAGATCTTGGCTTCCAACTACTGGTGGGCTTTACTACTTGGGATTGAAGAACTGA

mRNA sequence

ATGCTCGGGAAGGTGATCGTGTCGGGATTATCCCTTATTCTCGTTGTTGGTATAGCTCTAGCCATTGCCGCCACGATAAACAAAAGCAATGCTTCTAATGGTGGAACAGAGGACGTATCACCAAAGATGAAGGCAGTCTCAGCCATTTGTTCTACTACAGATTATCAAGATGAATGCAAAACTACACTTGACCATGTTGCTCGTAATACCTCTTCAAATGACCCCAAAGACTATGCTGAAGCAGCAATTCTTGCCACTATTGGGGAAATTACAAAAGGCTACAACTTGTCTGATAGCCTTATCGTGGAGGCCAGTACCAATGCCTCCATCAAAATGAGTGTTGAAGATTGCAAGGATTTGTTACAATTCGCCATTGATGAGTTGCAAGCATCGTATTCCGCTGTTGGAGAGTCTGATTTGCATACCGATGCAGATCGTGTTGCTGATATTAAGAATTGGCTTAGTGCTGTTATTTCTTATCAACAATCATGTCTTGATGGACTTGGAGAATTTGATCCTCAGCTTAAACAGAGGATGCAAGATGGTCTTGATGTTGCTGGCAAGCTTACCAGCAATGCTCTTGCTATTGTCACTGCAGTTTCCAATATCCTCGACAACTACCGCTTGCAATTGAAGGTTCAACCAAGTGGCCGTAGGCTTCTTGGAACAACAGTAGTAGATAGAGACGGGTTTCCGACATGGTTGACGGGGGCAGATAGGAAGTTGCTTGCATCGAAACAAGGAGGCGTTCGACCAACACCGAACGCAGTGGTGGCTAAGGACGGTAGTGGGAAATATAAGACCATTGCTGCTGCATTAGCTGCATACCCGAAGGTCCTAAGAGGAAGATACGTAATCTACGTGAAAGCAGGGATCTATGATGAATACATCACTCTAACTAAAGACATGAAAAATGTTTTCATGTATGGAGATGGTCCAAGAAAAACCATTGTCACGGGTCGCAAGAGCAACCGCGACGGTTTCACCACACAAAACACTGCCTCTTTTGCTGCCATAGGGGAAGGATTTCTTTGCAAATCCATGGGATTCACCAACACAGCTGGTCCTGAAGGCCACCAAGCAGTAGCCTTACGCGTTCAATCCGATAGATCCGCCTTCTTCAACTGCAGAATGGACGGTCATCAAGACACTCTCTACGTACAAACTCACCGTCAATTCTACCGCAACTGTGTCGTATCGGGTACCGTAGACTTCATCTTCGGTGATTCATCCACCGTCATCCAAAACTCCTTAATCATCGTTCGCCGCCCAATGGACAACCAACAAAACACCGTCACAGCCCAAGGTCGCGCCGAGCAAAAAGAAATCACCGGTCTAGTGATCCACAACTGCAGAATCGTCCCAGAACAGAAGTTGTTCGCTGAGAGGTTCAAGATTCCAACATTCTTGGGGCGACCATGGAAGCAATATGCGAGAACTGTGATCATGGAGTCTACATTGGGTGATTTCATTCAACCAGCTGGATACATGCCTTGGTCTGGAGATTTTGCATTGGAGACATGTTTGTATTTGGAGTATGGGAACAGAGGACCTGGTGCTAACACCAACAGAAGAGTGAGATGGAAGGGTGCTAAAGTGATTGGAAGAAATGAAGCTTTGCAATATACTGCTGGTGCATTTCTTCTCGGAAGATCTTGGCTTCCAACTACTGGTGGGCTTTACTACTTGGGATTGAAGAACTGA

Coding sequence (CDS)

ATGCTCGGGAAGGTGATCGTGTCGGGATTATCCCTTATTCTCGTTGTTGGTATAGCTCTAGCCATTGCCGCCACGATAAACAAAAGCAATGCTTCTAATGGTGGAACAGAGGACGTATCACCAAAGATGAAGGCAGTCTCAGCCATTTGTTCTACTACAGATTATCAAGATGAATGCAAAACTACACTTGACCATGTTGCTCGTAATACCTCTTCAAATGACCCCAAAGACTATGCTGAAGCAGCAATTCTTGCCACTATTGGGGAAATTACAAAAGGCTACAACTTGTCTGATAGCCTTATCGTGGAGGCCAGTACCAATGCCTCCATCAAAATGAGTGTTGAAGATTGCAAGGATTTGTTACAATTCGCCATTGATGAGTTGCAAGCATCGTATTCCGCTGTTGGAGAGTCTGATTTGCATACCGATGCAGATCGTGTTGCTGATATTAAGAATTGGCTTAGTGCTGTTATTTCTTATCAACAATCATGTCTTGATGGACTTGGAGAATTTGATCCTCAGCTTAAACAGAGGATGCAAGATGGTCTTGATGTTGCTGGCAAGCTTACCAGCAATGCTCTTGCTATTGTCACTGCAGTTTCCAATATCCTCGACAACTACCGCTTGCAATTGAAGGTTCAACCAAGTGGCCGTAGGCTTCTTGGAACAACAGTAGTAGATAGAGACGGGTTTCCGACATGGTTGACGGGGGCAGATAGGAAGTTGCTTGCATCGAAACAAGGAGGCGTTCGACCAACACCGAACGCAGTGGTGGCTAAGGACGGTAGTGGGAAATATAAGACCATTGCTGCTGCATTAGCTGCATACCCGAAGGTCCTAAGAGGAAGATACGTAATCTACGTGAAAGCAGGGATCTATGATGAATACATCACTCTAACTAAAGACATGAAAAATGTTTTCATGTATGGAGATGGTCCAAGAAAAACCATTGTCACGGGTCGCAAGAGCAACCGCGACGGTTTCACCACACAAAACACTGCCTCTTTTGCTGCCATAGGGGAAGGATTTCTTTGCAAATCCATGGGATTCACCAACACAGCTGGTCCTGAAGGCCACCAAGCAGTAGCCTTACGCGTTCAATCCGATAGATCCGCCTTCTTCAACTGCAGAATGGACGGTCATCAAGACACTCTCTACGTACAAACTCACCGTCAATTCTACCGCAACTGTGTCGTATCGGGTACCGTAGACTTCATCTTCGGTGATTCATCCACCGTCATCCAAAACTCCTTAATCATCGTTCGCCGCCCAATGGACAACCAACAAAACACCGTCACAGCCCAAGGTCGCGCCGAGCAAAAAGAAATCACCGGTCTAGTGATCCACAACTGCAGAATCGTCCCAGAACAGAAGTTGTTCGCTGAGAGGTTCAAGATTCCAACATTCTTGGGGCGACCATGGAAGCAATATGCGAGAACTGTGATCATGGAGTCTACATTGGGTGATTTCATTCAACCAGCTGGATACATGCCTTGGTCTGGAGATTTTGCATTGGAGACATGTTTGTATTTGGAGTATGGGAACAGAGGACCTGGTGCTAACACCAACAGAAGAGTGAGATGGAAGGGTGCTAAAGTGATTGGAAGAAATGAAGCTTTGCAATATACTGCTGGTGCATTTCTTCTCGGAAGATCTTGGCTTCCAACTACTGGTGGGCTTTACTACTTGGGATTGAAGAACTGA

Protein sequence

MLGKVIVSGLSLILVVGIALAIAATINKSNASNGGTEDVSPKMKAVSAICSTTDYQDECKTTLDHVARNTSSNDPKDYAEAAILATIGEITKGYNLSDSLIVEASTNASIKMSVEDCKDLLQFAIDELQASYSAVGESDLHTDADRVADIKNWLSAVISYQQSCLDGLGEFDPQLKQRMQDGLDVAGKLTSNALAIVTAVSNILDNYRLQLKVQPSGRRLLGTTVVDRDGFPTWLTGADRKLLASKQGGVRPTPNAVVAKDGSGKYKTIAAALAAYPKVLRGRYVIYVKAGIYDEYITLTKDMKNVFMYGDGPRKTIVTGRKSNRDGFTTQNTASFAAIGEGFLCKSMGFTNTAGPEGHQAVALRVQSDRSAFFNCRMDGHQDTLYVQTHRQFYRNCVVSGTVDFIFGDSSTVIQNSLIIVRRPMDNQQNTVTAQGRAEQKEITGLVIHNCRIVPEQKLFAERFKIPTFLGRPWKQYARTVIMESTLGDFIQPAGYMPWSGDFALETCLYLEYGNRGPGANTNRRVRWKGAKVIGRNEALQYTAGAFLLGRSWLPTTGGLYYLGLKN*

Homology

[Close]

BLAST of CSPI05G02400 vs. ExPASy Swiss-Prot
Match: Q3E8Z8 (Putative pectinesterase/pectinesterase inhibitor 28 OS=Arabidopsis thaliana OX=3702 GN=PME28 PE=2 SV=1)

HSP 1 Score: 444.9 bits (1143), Expect = 1.4e-123
Identity = 221/562 (39.32%), Postives = 348/562 (61.92%), Query Frame = 0


Query: 4   KVIVSGLSLILVVGIALAIAATINKSNASNGGTEDVSPKMKAVSAICSTTDYQDECKTTL 63
           + ++  +S +L++ + +A+   ++ + + N G E+++  +KA+  +C+ TDY++ C+ TL
Sbjct: 15  RYVIISISSVLLISMVVAVTIGVSVNKSDNAGDEEITTSVKAIKDVCAPTDYKETCEDTL 74

Query: 64  DHVARNTSSNDPKDYAEAAILATIGEITKGYNLSDSLIVEASTNASIKMSVEDCKDLLQF 123
              A++TS  DP +  + A  AT+ +I+     S ++I E   +   KM+++ CK+L+ +
Sbjct: 75  RKDAKDTS--DPLELVKTAFNATMKQISDVAKKSQTMI-ELQKDPRAKMALDQCKELMDY 134

Query: 124 AIDELQASYSAVGESDLHTDADRVADIKNWLSAVISYQQSCLDGLGEFDPQLKQRMQDGL 183
           AI EL  S+  +G+ + H   + +  ++ WLSA IS++Q+CLDG         + ++  L
Sbjct: 135 AIGELSKSFEELGKFEFHKVDEALVKLRIWLSATISHEQTCLDGFQGTQGNAGETIKKAL 194

Query: 184 DVAGKLTSNALAIVTAVSNILDNYRLQLKVQPSGRRLLGTTVVDRDGFPTWLTGADRKLL 243
             A +LT N LA+VT +SN L   ++    + + RRLL         FP+W+    R+LL
Sbjct: 195 KTAVQLTHNGLAMVTEMSNYLGQMQIP---EMNSRRLLS------QEFPSWMDARARRLL 254

Query: 244 ASKQGGVRPTPNAVVAKDGSGKYKTIAAALAAYPKVLRGRYVIYVKAGIYDEYITLTKDM 303
            +    V+  P+ VVA+DGSG+YKTI  AL   PK     +V+++K GIY EY+ + + M
Sbjct: 255 NAPMSEVK--PDIVVAQDGSGQYKTINEALNFVPKKKNTTFVVHIKEGIYKEYVQVNRSM 314

Query: 304 KNVFMYGDGPRKTIVTGRKSNRDGFTTQNTASFAAIGEGFLCKSMGFTNTAGPEGHQAVA 363
            ++   GDGP KT+++G KS +DG TT  TA+ A +G+ F+ K++ F NTAG   HQAVA
Sbjct: 315 THLVFIGDGPDKTVISGSKSYKDGITTYKTATVAIVGDHFIAKNIAFENTAGAIKHQAVA 374

Query: 364 LRVQSDRSAFFNCRMDGHQDTLYVQTHRQFYRNCVVSGTVDFIFGDSSTVIQNSLIIVRR 423
           +RV +D S F+NC+ DG+QDTLY  +HRQFYR+C +SGT+DF+FGD++ V QN  ++VR+
Sbjct: 375 IRVLADESIFYNCKFDGYQDTLYAHSHRQFYRDCTISGTIDFLFGDAAAVFQNCTLLVRK 434

Query: 424 PMDNQQNTVTAQGRAEQKEITGLVIHNCRIVPEQKLFAERFKIPTFLGRPWKQYARTVIM 483
           P+ NQ   +TA GR + +E TG V+  C IV E    A + +  T+LGRPWK+Y+RT+IM
Sbjct: 435 PLLNQACPITAHGRKDPRESTGFVLQGCTIVGEPDYLAVKEQSKTYLGRPWKEYSRTIIM 494

Query: 484 ESTLGDFIQPAGYMPWSGDFALETCLYLEYGNRGPGANTNRRVRWKGAKVIGRNEALQYT 543
            + + DF+ P G+ PW G+F L T  Y E  N GPGA   +RV W G K +   E L++T
Sbjct: 495 NTFIPDFVPPEGWQPWLGEFGLNTLFYSEVQNTGPGAAITKRVTWPGIKKLSDEEILKFT 554

Query: 544 AGAFLLGRSWLPTTGGLYYLGL 566
              ++ G +W+P  G  Y LGL
Sbjct: 555 PAQYIQGDAWIPGKGVPYILGL 562

[Close]

BLAST of CSPI05G02400 vs. ExPASy Swiss-Prot
Match: Q8GX86 (Probable pectinesterase/pectinesterase inhibitor 21 OS=Arabidopsis thaliana OX=3702 GN=PME21 PE=2 SV=2)

HSP 1 Score: 442.6 bits (1137), Expect = 6.8e-123
Identity = 223/561 (39.75%), Postives = 343/561 (61.14%), Query Frame = 0


Query: 5   VIVSGLSLILVVGIALAIAATINKSNASNGGTEDVSPKMKAVSAICSTTDYQDECKTTLD 64
           + +S + LI +V +A+ +  ++NK +  + G  +V+  +KAV  +C+ TDY+  C+ TL 
Sbjct: 18  ITISSVLLISMV-VAVTVGVSLNKHDGDSKGKAEVNASVKAVKDVCAPTDYRKTCEDTLI 77

Query: 65  HVARNTSSNDPKDYAEAAILATIGEITKGYNLSDSLIVEASTNASIKMSVEDCKDLLQFA 124
              +NT+  DP +  + A   T+ +IT     S + I+E   ++  +M+++ CK+L+ +A
Sbjct: 78  KNGKNTT--DPMELVKTAFNVTMKQITDAAKKSQT-IMELQKDSRTRMALDQCKELMDYA 137

Query: 125 IDELQASYSAVGESDLHTDADRVADIKNWLSAVISYQQSCLDGLGEFDPQLKQRMQDGLD 184
           +DEL  S+  +G+ + H   + + +++ WLSA IS++++CL+G         + M+  L 
Sbjct: 138 LDELSNSFEELGKFEFHLLDEALINLRIWLSAAISHEETCLEGFQGTQGNAGETMKKALK 197

Query: 185 VAGKLTSNALAIVTAVSNILDNYRLQLKVQPSGRRLLGTTVVDRDGFPTWLTGADRKLLA 244
            A +LT N LAI++ +SN +   ++      + RRLL       +GFP+W+    RKLL 
Sbjct: 198 TAIELTHNGLAIISEMSNFVGQMQIP---GLNSRRLLA------EGFPSWVDQRGRKLLQ 257

Query: 245 SKQGGVRPTPNAVVAKDGSGKYKTIAAALAAYPKVLRGRYVIYVKAGIYDEYITLTKDMK 304
           +        P+ VVA+DGSG+YKTI  AL   PK     +V+++KAG+Y EY+ + K M 
Sbjct: 258 AAAAYSDVKPDIVVAQDGSGQYKTINEALQFVPKKRNTTFVVHIKAGLYKEYVQVNKTMS 317

Query: 305 NVFMYGDGPRKTIVTGRKSNRDGFTTQNTASFAAIGEGFLCKSMGFTNTAGPEGHQAVAL 364
           ++   GDGP KTI++G K+ +DG TT  TA+ A +G  F+ K++GF NTAG   HQAVA+
Sbjct: 318 HLVFIGDGPDKTIISGNKNYKDGITTYRTATVAIVGNYFIAKNIGFENTAGAIKHQAVAV 377

Query: 365 RVQSDRSAFFNCRMDGHQDTLYVQTHRQFYRNCVVSGTVDFIFGDSSTVIQNSLIIVRRP 424
           RVQSD S FFNCR DG+QDTLY  +HRQF+R+C +SGT+DF+FGD++ V QN  ++VR+P
Sbjct: 378 RVQSDESIFFNCRFDGYQDTLYTHSHRQFFRDCTISGTIDFLFGDAAAVFQNCTLLVRKP 437

Query: 425 MDNQQNTVTAQGRAEQKEITGLVIHNCRIVPEQKLFAERFKIPTFLGRPWKQYARTVIME 484
           + NQ   +TA GR + +E TG V   C I  E    A +     +LGRPWK+Y+RT+IM 
Sbjct: 438 LPNQACPITAHGRKDPRESTGFVFQGCTIAGEPDYLAVKETSKAYLGRPWKEYSRTIIMN 497

Query: 485 STLGDFIQPAGYMPWSGDFALETCLYLEYGNRGPGANTNRRVRWKGAKVIGRNEALQYTA 544
           + + DF+QP G+ PW GDF L+T  Y E  N GPG+    RV W G K +   + L++T 
Sbjct: 498 TFIPDFVQPQGWQPWLGDFGLKTLFYSEVQNTGPGSALANRVTWAGIKTLSEEDILKFTP 557

Query: 545 GAFLLGRSWLPTTGGLYYLGL 566
             ++ G  W+P  G  Y  GL
Sbjct: 558 AQYIQGDDWIPGKGVPYTTGL 565

[Close]

BLAST of CSPI05G02400 vs. ExPASy Swiss-Prot
Match: Q9SMY6 (Putative pectinesterase/pectinesterase inhibitor 45 OS=Arabidopsis thaliana OX=3702 GN=PME45 PE=2 SV=1)

HSP 1 Score: 427.9 bits (1099), Expect = 1.7e-118
Identity = 248/600 (41.33%), Postives = 354/600 (59.00%), Query Frame = 0


Query: 4   KVIVSGLSLILVVGIALAIAA----------------TINKSNASNGGTEDVSPKM---- 63
           K I+ G+  +LVV  A+   A                T NKS  S   +E  SPK     
Sbjct: 23  KRIILGVVSVLVVAAAIIGGAFAYVTYENKTQEQGKTTNNKSKDSPTKSESPSPKPPSSA 82

Query: 64  ----------KAVSAICSTTDYQDECKTTL-DHVARNTSSNDPKDYAEAAILATIGEITK 123
                     K +  +C++T Y+  C+ TL +   ++T   DP+   ++AI+A   ++ +
Sbjct: 83  AQTVKAGQVDKIIQTLCNSTLYKPTCQNTLKNETKKDTPQTDPRSLLKSAIVAVNDDLDQ 142

Query: 124 GYNLSDSLIVEASTNASIKMSVEDCKDLLQFAIDELQASYSAVGESDLHTDADRVADIKN 183
            +    SL  E   N   K ++  CK L+  A +EL  S   + +S+++  A  V D+ +
Sbjct: 143 VFKRVLSLKTE---NKDDKDAIAQCKLLVDEAKEELGTSMKRINDSEVNNFAKIVPDLDS 202

Query: 184 WLSAVISYQQSCLDGLGEFDPQLKQRMQDGLDVAGKLTSNALAIVTAVSNILDNY----- 243
           WLSAV+SYQ++C+DG  E   +LK  ++   + +  LTSN+LA++ ++   L +      
Sbjct: 203 WLSAVMSYQETCVDGFEE--GKLKTEIRKNFNSSQVLTSNSLAMIKSLDGYLSSVPKVKT 262

Query: 244 RLQLKVQPSGRRLLGTTVVDRDGFPTWLTGADRKLLASKQGGVRP-TPNAVVAKDGSGKY 303
           RL L+ + S +        + D   +WL+  +R++L  K   V+   PNA VAKDGSG +
Sbjct: 263 RLLLEARSSAK--------ETDHITSWLSNKERRML--KAVDVKALKPNATVAKDGSGNF 322

Query: 304 KTIAAALAAYPKVLRGRYVIYVKAGIYDEYITLTKDMKNVFMYGDGPRKTIVTGRKSNRD 363
            TI AAL A P   +GRY IY+K GIYDE + + K   NV M GDG +KTIVTG KS+  
Sbjct: 323 TTINAALKAMPAKYQGRYTIYIKHGIYDESVIIDKKKPNVTMVGDGSQKTIVTGNKSHAK 382

Query: 364 GFTTQNTASFAAIGEGFLCKSMGFTNTAGPEGHQAVALRVQSDRSAFFNCRMDGHQDTLY 423
              T  TA+F A GEGF+ +SMGF NTAGPEGHQAVA+RVQSDRS F NCR +G+QDTLY
Sbjct: 383 KIRTFLTATFVAQGEGFMAQSMGFRNTAGPEGHQAVAIRVQSDRSVFLNCRFEGYQDTLY 442

Query: 424 VQTHRQFYRNCVVSGTVDFIFGDSSTVIQNSLIIVRRPMDNQQNTVTAQGRAEQKEITGL 483
             THRQ+YR+CV+ GTVDFIFGD++ + QN  I +R+ +  Q+NTVTAQGR ++ + TG 
Sbjct: 443 AYTHRQYYRSCVIIGTVDFIFGDAAAIFQNCDIFIRKGLPGQKNTVTAQGRVDKFQTTGF 502

Query: 484 VIHNCRIVPEQKLFAERFKIPTFLGRPWKQYARTVIMESTLGDFIQPAGYMPW-SGDFAL 543
           VIHNC + P + L   + +  ++LGRPWK ++RTV+MEST+ D I P G++ W   DFA+
Sbjct: 503 VIHNCTVAPNEDLKPVKAQFKSYLGRPWKPHSRTVVMESTIEDVIDPVGWLRWQETDFAI 562

Query: 544 ETCLYLEYGNRGPGANTNRRVRWKGAKVIGRNEALQYTAGAFLLGRSWLPTTGGLYYLGL 566
           +T  Y EY N GP   T  RV+W G +V+ + EA+++T G FL G  W+   G    LGL
Sbjct: 563 DTLSYAEYKNDGPSGATAARVKWPGFRVLNKEEAMKFTVGPFLQG-EWIQAIGSPVKLGL 606

[Close]

BLAST of CSPI05G02400 vs. ExPASy Swiss-Prot
Match: O80722 (Pectinesterase 4 OS=Arabidopsis thaliana OX=3702 GN=PME4 PE=2 SV=1)

HSP 1 Score: 427.9 bits (1099), Expect = 1.7e-118
Identity = 255/604 (42.22%), Postives = 358/604 (59.27%), Query Frame = 0


Query: 1   MLGKVIVSGLSLILVVGIALAIAATINKSNASNGGTEDVSPKMKAVSAICSTTDYQDECK 60
           M+GKV+VS  S++L+VG+A+ + A INK+     G  ++SP+MKAV  IC +T  +  C 
Sbjct: 1   MIGKVVVSVASILLIVGVAIGVVAFINKN-----GDANLSPQMKAVQGICQSTSDKASCV 60

Query: 61  TTLDHVARNTSSNDPKDYAEAAILATIGEITKGYNLSDSLIVE--ASTNASIKMSVEDCK 120
            TL+ V     S DP    +A +LAT  E+TK  N +    V   +S + + K  ++ CK
Sbjct: 61  KTLEPV----KSEDPNKLIKAFMLATKDELTKSSNFTGQTEVNMGSSISPNNKAVLDYCK 120

Query: 121 DLLQFAIDELQASYSAVGESDLHTDADRVADIKNWLSAVISYQQSCLDGLGEFDPQLKQR 180
            +  +A+++L      +GE DL     ++  +K WL  V +YQ  CLD + E D  L++ 
Sbjct: 121 RVFMYALEDLATIIEEMGE-DLSQIGSKIDQLKQWLIGVYNYQTDCLDDIEEDD--LRKA 180

Query: 181 MQDGLDVAGKLTSNALAI-------VTAVSNILDNYRLQLKVQPS--------------- 240
           + +G+  +  LT+NA+ I       +  ++N +D+ +      P+               
Sbjct: 181 IGEGIANSKILTTNAIDIFHTVVSAMAKINNKVDDLKNMTGGIPTPGAPPVVDESPVADP 240

Query: 241 ---GRRLLGTTVVDRDGFPTWLTGADRKLLA--------SKQGGVRPTPNAVVAKDGSGK 300
               RRLL    +D  G PTW++GADRKL+A         + GG R   N VVAKDGSG+
Sbjct: 241 DGPARRLLED--IDETGIPTWVSGADRKLMAKAGRGRRGGRGGGARVRTNFVVAKDGSGQ 300

Query: 301 YKTIAAALAAYPKVLRGRYVIYVKAGIYDEYITLTKDMKNVFMYGDGPRKTIVTGRKS-- 360
           +KT+  A+ A P+  RGR +IY+KAG+Y E + + K   N+FM+GDG RKT+++  +S  
Sbjct: 301 FKTVQQAVDACPENNRGRCIIYIKAGLYREQVIIPKKKNNIFMFGDGARKTVISYNRSVA 360

Query: 361 NRDGFTTQNTASFAAIGEGFLCKSMGFTNTAGPEGHQAVALRVQSDRSAFFNCRMDGHQD 420
              G TT  +A+     EGF+ K MGF NTAGP GHQA A+RV  DR+  FNCR DG+QD
Sbjct: 361 LSRGTTTSLSATVQVESEGFMAKWMGFKNTAGPMGHQAAAIRVNGDRAVIFNCRFDGYQD 420

Query: 421 TLYVQTHRQFYRNCVVSGTVDFIFGDSSTVIQNSLIIVRRPMDNQQNTVTAQGRAEQKEI 480
           TLYV   RQFYRNCVVSGTVDFIFG S+TVIQN+LI+VR+    Q NTVTA G      +
Sbjct: 421 TLYVNNGRQFYRNCVVSGTVDFIFGKSATVIQNTLIVVRKGSKGQYNTVTADGNELGLGM 480

Query: 481 -TGLVIHNCRIVPEQKLFAERFKIPTFLGRPWKQYARTVIMESTLGDFIQPAGYMPWSGD 540
             G+V+ NCRIVP++KL  ER  + T+LGRPWK+++ TVIM + +GD I+P G+  W G+
Sbjct: 481 KIGIVLQNCRIVPDRKLTPERLTVATYLGRPWKKFSTTVIMSTEMGDLIRPEGWKIWDGE 540

Query: 541 FALETCLYLEYGNRGPGANTNRRVRWKGAKVI-GRNEALQYTAGAFLLGRSWL-----PT 561
              ++C Y+EY NRGPGA  NRRV W  AKV     E   +TA  +L   +W+     P 
Sbjct: 541 SFHKSCRYVEYNNRGPGAFANRRVNW--AKVARSAAEVNGFTAANWLGPINWIQEANVPV 588

[Close]

BLAST of CSPI05G02400 vs. ExPASy Swiss-Prot
Match: Q7Y201 (Probable pectinesterase/pectinesterase inhibitor 13 OS=Arabidopsis thaliana OX=3702 GN=PME13 PE=2 SV=2)

HSP 1 Score: 424.9 bits (1091), Expect = 1.5e-117
Identity = 228/524 (43.51%), Postives = 330/524 (62.98%), Query Frame = 0


Query: 44  KAVSAICSTTDYQDECKTTL-DHVARNTSSNDPKDYAEAAILATIGEITKGYNLSDSLIV 103
           K +  +CS+T Y   C+ TL +   +  + ++P  + ++AI A   ++     + + ++ 
Sbjct: 107 KIIQTLCSSTLYMQICEKTLKNRTDKGFALDNPTTFLKSAIEAVNEDLDL---VLEKVLS 166

Query: 104 EASTNASIKMSVEDCKDLLQFAIDELQASYSAVGESDLHTDADRVADIKNWLSAVISYQQ 163
             + N   K ++E CK L++ A +E  AS + +  +++++    V D+++WLSAV+SYQ+
Sbjct: 167 LKTENQDDKDAIEQCKLLVEDAKEETVASLNKINVTEVNSFEKVVPDLESWLSAVMSYQE 226

Query: 164 SCLDGLGEFDPQLKQRMQDGLDVAGKLTSNALAIVTAVSNILDNYRLQLKVQPSGRRLLG 223
           +CLDG  E    LK  ++  ++ +  LTSN+LA+   +    +N    +KV    R LL 
Sbjct: 227 TCLDGFEE--GNLKSEVKTSVNSSQVLTSNSLAL---IKTFTENLSPVMKVVE--RHLL- 286

Query: 224 TTVVDRDGFPTWLTGADRKLLASKQGGVRPTPNAVVAKDGSGKYKTIAAALAAYPKVLRG 283
                 D  P+W++  DR++L +        PNA VAKDGSG + TI  AL A P+   G
Sbjct: 287 ------DDIPSWVSNDDRRMLRAVDVKAL-KPNATVAKDGSGDFTTINDALRAMPEKYEG 346

Query: 284 RYVIYVKAGIYDEYITLTKDMKNVFMYGDGPRKTIVTGRKSNRDGFTTQNTASFAAIGEG 343
           RY+IYVK GIYDEY+T+ K   N+ M GDG +KTIVTG KS+     T  TA+F A GEG
Sbjct: 347 RYIIYVKQGIYDEYVTVDKKKANLTMVGDGSQKTIVTGNKSHAKKIRTFLTATFVAQGEG 406

Query: 344 FLCKSMGFTNTAGPEGHQAVALRVQSDRSAFFNCRMDGHQDTLYVQTHRQFYRNCVVSGT 403
           F+ +SMGF NTAGPEGHQAVA+RVQSDRS F NCR +G+QDTLY  THRQ+YR+CV+ GT
Sbjct: 407 FMAQSMGFRNTAGPEGHQAVAIRVQSDRSIFLNCRFEGYQDTLYAYTHRQYYRSCVIVGT 466

Query: 404 VDFIFGDSSTVIQNSLIIVRRPMDNQQNTVTAQGRAEQKEITGLVIHNCRIVPEQKLFAE 463
           +DFIFGD++ + QN  I +R+ +  Q+NTVTAQGR ++ + TG V+HNC+I   + L   
Sbjct: 467 IDFIFGDAAAIFQNCNIFIRKGLPGQKNTVTAQGRVDKFQTTGFVVHNCKIAANEDLKPV 526

Query: 464 RFKIPTFLGRPWKQYARTVIMESTLGDFIQPAGYMPW-SGDFALETCLYLEYGNRGPGAN 523
           + +  ++LGRPWK Y+RT+IMES + + I P G++ W   DFA++T  Y EY N+G   +
Sbjct: 527 KEEYKSYLGRPWKNYSRTIIMESKIENVIDPVGWLRWQETDFAIDTLYYAEYNNKGSSGD 586

Query: 524 TNRRVRWKGAKVIGRNEALQYTAGAFLLGRSWLPTTGGLYYLGL 566
           T  RV+W G KVI + EAL YT G FL G  W+  +G    LGL
Sbjct: 587 TTSRVKWPGFKVINKEEALNYTVGPFLQG-DWISASGSPVKLGL 611

[Close]

BLAST of CSPI05G02400 vs. ExPASy TrEMBL
Match: A0A0A0KJ73 (Pectinesterase OS=Cucumis sativus OX=3659 GN=Csa_5G125700 PE=3 SV=1)

HSP 1 Score: 1122.8 bits (2903), Expect = 0.0e+00
Identity = 565/567 (99.65%), Postives = 566/567 (99.82%), Query Frame = 0


Query: 1   MLGKVIVSGLSLILVVGIALAIAATINKSNASNGGTEDVSPKMKAVSAICSTTDYQDECK 60
           MLGKVIVSGLSLILVVGIALAIAATINKSNASNGGTEDVSPKMKAVSAICSTTDYQDECK
Sbjct: 1   MLGKVIVSGLSLILVVGIALAIAATINKSNASNGGTEDVSPKMKAVSAICSTTDYQDECK 60

Query: 61  TTLDHVARNTSSNDPKDYAEAAILATIGEITKGYNLSDSLIVEASTNASIKMSVEDCKDL 120
           TTLDHVARNTSSNDPKDYAEAAILATIGEITKGYNLSDSLIVEASTNASIKMSVEDCKDL
Sbjct: 61  TTLDHVARNTSSNDPKDYAEAAILATIGEITKGYNLSDSLIVEASTNASIKMSVEDCKDL 120

Query: 121 LQFAIDELQASYSAVGESDLHTDADRVADIKNWLSAVISYQQSCLDGLGEFDPQLKQRMQ 180
           LQFAIDELQASYSAVGESDLHTD+DRVADIKNWLSAVISYQQSCLDGLGEFDPQLKQRMQ
Sbjct: 121 LQFAIDELQASYSAVGESDLHTDSDRVADIKNWLSAVISYQQSCLDGLGEFDPQLKQRMQ 180

Query: 181 DGLDVAGKLTSNALAIVTAVSNILDNYRLQLKVQPSGRRLLGTTVVDRDGFPTWLTGADR 240
           DGLDVAGKLTSNALAIVTAVSNILDNYRLQLKVQPSGRRLLGTTVVDRDGFPTWLTGADR
Sbjct: 181 DGLDVAGKLTSNALAIVTAVSNILDNYRLQLKVQPSGRRLLGTTVVDRDGFPTWLTGADR 240

Query: 241 KLLASKQGGVRPTPNAVVAKDGSGKYKTIAAALAAYPKVLRGRYVIYVKAGIYDEYITLT 300
           KLLASKQ GVRPTPNAVVAKDGSGKYKTIAAALAAYPKVLRGRYVIYVKAGIYDEYITLT
Sbjct: 241 KLLASKQRGVRPTPNAVVAKDGSGKYKTIAAALAAYPKVLRGRYVIYVKAGIYDEYITLT 300

Query: 301 KDMKNVFMYGDGPRKTIVTGRKSNRDGFTTQNTASFAAIGEGFLCKSMGFTNTAGPEGHQ 360
           KDMKNVFMYGDGPRKTIVTGRKSNRDGFTTQNTASFAAIGEGFLCKSMGFTNTAGPEGHQ
Sbjct: 301 KDMKNVFMYGDGPRKTIVTGRKSNRDGFTTQNTASFAAIGEGFLCKSMGFTNTAGPEGHQ 360

Query: 361 AVALRVQSDRSAFFNCRMDGHQDTLYVQTHRQFYRNCVVSGTVDFIFGDSSTVIQNSLII 420
           AVALRVQSDRSAFFNCRMDGHQDTLYVQTHRQFYRNCVVSGTVDFIFGDSSTVIQNSLII
Sbjct: 361 AVALRVQSDRSAFFNCRMDGHQDTLYVQTHRQFYRNCVVSGTVDFIFGDSSTVIQNSLII 420

Query: 421 VRRPMDNQQNTVTAQGRAEQKEITGLVIHNCRIVPEQKLFAERFKIPTFLGRPWKQYART 480
           VRRPMDNQQNTVTAQGRAEQKEITGLVIHNCRIVPEQKLFAERFKIPTFLGRPWKQYART
Sbjct: 421 VRRPMDNQQNTVTAQGRAEQKEITGLVIHNCRIVPEQKLFAERFKIPTFLGRPWKQYART 480

Query: 481 VIMESTLGDFIQPAGYMPWSGDFALETCLYLEYGNRGPGANTNRRVRWKGAKVIGRNEAL 540
           VIMESTLGDFIQPAGYMPWSGDFALETCLYLEYGNRGPGANTNRRVRWKGAKVIGRNEAL
Sbjct: 481 VIMESTLGDFIQPAGYMPWSGDFALETCLYLEYGNRGPGANTNRRVRWKGAKVIGRNEAL 540

Query: 541 QYTAGAFLLGRSWLPTTGGLYYLGLKN 568
           QYTAGAFLLGRSWLPTTGGLYYLGLKN
Sbjct: 541 QYTAGAFLLGRSWLPTTGGLYYLGLKN 567

[Close]

BLAST of CSPI05G02400 vs. ExPASy TrEMBL
Match: A0A5A7U4V7 (Pectinesterase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold180G00110 PE=3 SV=1)

HSP 1 Score: 1075.1 bits (2779), Expect = 0.0e+00
Identity = 533/567 (94.00%), Postives = 550/567 (97.00%), Query Frame = 0


Query: 1   MLGKVIVSGLSLILVVGIALAIAATINKSNASNGGTEDVSPKMKAVSAICSTTDYQDECK 60
           M+GKVIVS LSLILVVGIALA+ ATINK N SNGG E+VSPKMKA+SAICSTTDYQ+ECK
Sbjct: 1   MVGKVIVSALSLILVVGIALAVVATINKRNGSNGGKEEVSPKMKAISAICSTTDYQEECK 60

Query: 61  TTLDHVARNTSSNDPKDYAEAAILATIGEITKGYNLSDSLIVEASTNASIKMSVEDCKDL 120
            TL+HVA NTSSNDPKDYA+AAILATI EITKGYNLSDSLIVEA  NASIKMSVEDCKDL
Sbjct: 61  NTLNHVAHNTSSNDPKDYAKAAILATIKEITKGYNLSDSLIVEAGNNASIKMSVEDCKDL 120

Query: 121 LQFAIDELQASYSAVGESDLHTDADRVADIKNWLSAVISYQQSCLDGLGEFDPQLKQRMQ 180
           LQFAIDELQASYS VGESDLHTDADR+ADIKNWLSAV+SYQQSCLDGLGEFDPQLKQRMQ
Sbjct: 121 LQFAIDELQASYSTVGESDLHTDADRIADIKNWLSAVVSYQQSCLDGLGEFDPQLKQRMQ 180

Query: 181 DGLDVAGKLTSNALAIVTAVSNILDNYRLQLKVQPSGRRLLGTTVVDRDGFPTWLTGADR 240
           DGLDVAGKLTSNALAIVTAVSNILDNY LQLKVQPSGRRLLGTT VD DGFPTWLTGADR
Sbjct: 181 DGLDVAGKLTSNALAIVTAVSNILDNYGLQLKVQPSGRRLLGTTEVDSDGFPTWLTGADR 240

Query: 241 KLLASKQGGVRPTPNAVVAKDGSGKYKTIAAALAAYPKVLRGRYVIYVKAGIYDEYITLT 300
           KLLASKQGGVRPTPNAVVAKDGSG+YKTIAAALAAYPK LRGRYVIYVKAGIYDEYIT+T
Sbjct: 241 KLLASKQGGVRPTPNAVVAKDGSGQYKTIAAALAAYPKALRGRYVIYVKAGIYDEYITVT 300

Query: 301 KDMKNVFMYGDGPRKTIVTGRKSNRDGFTTQNTASFAAIGEGFLCKSMGFTNTAGPEGHQ 360
           KDMKNVFMYGDGPRKTIVTGRKSNRDGFTTQNTASFAAIGEGFLCKSMGFTNTAGP+GHQ
Sbjct: 301 KDMKNVFMYGDGPRKTIVTGRKSNRDGFTTQNTASFAAIGEGFLCKSMGFTNTAGPQGHQ 360

Query: 361 AVALRVQSDRSAFFNCRMDGHQDTLYVQTHRQFYRNCVVSGTVDFIFGDSSTVIQNSLII 420
           AVALRVQSDRSAFFNCRMDGHQDTLYVQTHRQFYRNCV+SGTVDFIFGDSST+IQNSLII
Sbjct: 361 AVALRVQSDRSAFFNCRMDGHQDTLYVQTHRQFYRNCVISGTVDFIFGDSSTIIQNSLII 420

Query: 421 VRRPMDNQQNTVTAQGRAEQKEITGLVIHNCRIVPEQKLFAERFKIPTFLGRPWKQYART 480
           VRRPMDNQQNTVTAQGRAEQKEITGLVIHNCRIVPEQKLFAERFKIPTFLGRPWKQYART
Sbjct: 421 VRRPMDNQQNTVTAQGRAEQKEITGLVIHNCRIVPEQKLFAERFKIPTFLGRPWKQYART 480

Query: 481 VIMESTLGDFIQPAGYMPWSGDFALETCLYLEYGNRGPGANTNRRVRWKGAKVIGRNEAL 540
           VIMESTLGDFIQPAGYMPWSG+FALETCLYLEYGNRGPGANTNRRVRWKG KVIGRNEAL
Sbjct: 481 VIMESTLGDFIQPAGYMPWSGNFALETCLYLEYGNRGPGANTNRRVRWKGVKVIGRNEAL 540

Query: 541 QYTAGAFLLGRSWLPTTGGLYYLGLKN 568
           QYTAGAFLLGR+WLP TGG+YYLGLKN
Sbjct: 541 QYTAGAFLLGRTWLPRTGGIYYLGLKN 567

[Close]

BLAST of CSPI05G02400 vs. ExPASy TrEMBL
Match: A0A1S3CSH2 (Pectinesterase OS=Cucumis melo OX=3656 GN=LOC103503817 PE=3 SV=1)

HSP 1 Score: 1072.4 bits (2772), Expect = 6.3e-310
Identity = 531/567 (93.65%), Postives = 550/567 (97.00%), Query Frame = 0


Query: 1   MLGKVIVSGLSLILVVGIALAIAATINKSNASNGGTEDVSPKMKAVSAICSTTDYQDECK 60
           M+GKVIVS LSLILVVGIALA+ ATINK N SNGG E+VSPKMKA+SAICSTTDYQ+ECK
Sbjct: 1   MVGKVIVSALSLILVVGIALAVVATINKRNGSNGGKEEVSPKMKAISAICSTTDYQEECK 60

Query: 61  TTLDHVARNTSSNDPKDYAEAAILATIGEITKGYNLSDSLIVEASTNASIKMSVEDCKDL 120
            TL+HVA+NTSSNDPKDYA+AAILATI EITKGYNLSDSLIVEA  NASIKMSVEDC DL
Sbjct: 61  NTLNHVAQNTSSNDPKDYAKAAILATIKEITKGYNLSDSLIVEAGNNASIKMSVEDCNDL 120

Query: 121 LQFAIDELQASYSAVGESDLHTDADRVADIKNWLSAVISYQQSCLDGLGEFDPQLKQRMQ 180
           LQFAIDELQASYS VGESDLHTDADR+ADIKNWLSAV+SYQQSCLDGLGEFDPQLKQRMQ
Sbjct: 121 LQFAIDELQASYSTVGESDLHTDADRIADIKNWLSAVVSYQQSCLDGLGEFDPQLKQRMQ 180

Query: 181 DGLDVAGKLTSNALAIVTAVSNILDNYRLQLKVQPSGRRLLGTTVVDRDGFPTWLTGADR 240
           DGLDVAGKLTSNALAI+TAVSNILDNY LQLKVQPSGRRLLGTT VD DGFPTWLTGADR
Sbjct: 181 DGLDVAGKLTSNALAILTAVSNILDNYGLQLKVQPSGRRLLGTTEVDSDGFPTWLTGADR 240

Query: 241 KLLASKQGGVRPTPNAVVAKDGSGKYKTIAAALAAYPKVLRGRYVIYVKAGIYDEYITLT 300
           KLLASKQGGVRPTPNAVVAKDGSG+YKTIAAALAAYPK LRGRYVIYVKAGIYDEYIT+T
Sbjct: 241 KLLASKQGGVRPTPNAVVAKDGSGQYKTIAAALAAYPKALRGRYVIYVKAGIYDEYITVT 300

Query: 301 KDMKNVFMYGDGPRKTIVTGRKSNRDGFTTQNTASFAAIGEGFLCKSMGFTNTAGPEGHQ 360
           KDMKNVFMYGDGPRKTIVTGRKSNRDGFTTQNTASFAAIGEGFLCKSMGFTNTAGP+GHQ
Sbjct: 301 KDMKNVFMYGDGPRKTIVTGRKSNRDGFTTQNTASFAAIGEGFLCKSMGFTNTAGPQGHQ 360

Query: 361 AVALRVQSDRSAFFNCRMDGHQDTLYVQTHRQFYRNCVVSGTVDFIFGDSSTVIQNSLII 420
           AVALRVQSDRSAFFNCRMDGHQDTLYVQTHRQFYRNCV+SGTVDFIFGDSST+IQNSLII
Sbjct: 361 AVALRVQSDRSAFFNCRMDGHQDTLYVQTHRQFYRNCVISGTVDFIFGDSSTIIQNSLII 420

Query: 421 VRRPMDNQQNTVTAQGRAEQKEITGLVIHNCRIVPEQKLFAERFKIPTFLGRPWKQYART 480
           VRRPMDNQQNTVTAQGRAEQKEITGLVIHNCRIVPEQKLFAERFKIPTFLGRPWKQYART
Sbjct: 421 VRRPMDNQQNTVTAQGRAEQKEITGLVIHNCRIVPEQKLFAERFKIPTFLGRPWKQYART 480

Query: 481 VIMESTLGDFIQPAGYMPWSGDFALETCLYLEYGNRGPGANTNRRVRWKGAKVIGRNEAL 540
           VIMESTLGDFIQPAGYMPWSG+FALETCLYLEYGNRGPGANTNRRVRWKG KVIGRNEAL
Sbjct: 481 VIMESTLGDFIQPAGYMPWSGNFALETCLYLEYGNRGPGANTNRRVRWKGVKVIGRNEAL 540

Query: 541 QYTAGAFLLGRSWLPTTGGLYYLGLKN 568
           QYTAGAFLLGR+WLP TGG+YYLGLKN
Sbjct: 541 QYTAGAFLLGRTWLPRTGGIYYLGLKN 567

[Close]

BLAST of CSPI05G02400 vs. ExPASy TrEMBL
Match: A0A0A0KLN2 (Pectinesterase OS=Cucumis sativus OX=3659 GN=Csa_5G119700 PE=3 SV=1)

HSP 1 Score: 938.7 bits (2425), Expect = 1.1e-269
Identity = 461/567 (81.31%), Postives = 517/567 (91.18%), Query Frame = 0


Query: 1   MLGKVIVSGLSLILVVGIALAIAATINKSNASNGGTEDVSPKMKAVSAICSTTDYQDECK 60
           M+GKV++SG+SLILVVG+ALA+ A +NKS++S+  T ++SPKMKAV++ICS  DYQ EC+
Sbjct: 1   MVGKVVMSGISLILVVGVALAVVAIVNKSDSSD-ETANLSPKMKAVASICSNADYQPECQ 60

Query: 61  TTLDHVARNTSSNDPKDYAEAAILATIGEITKGYNLSDSLIVEASTNASIKMSVEDCKDL 120
           TTL  V  N+S  DPK++ +AAI +TI E+ KGYNL+D+L+VEA+ NA+IKMSV+DCKDL
Sbjct: 61  TTLGSVGNNSSVEDPKEFIKAAITSTIEEMKKGYNLTDNLMVEAANNATIKMSVDDCKDL 120

Query: 121 LQFAIDELQASYSAVGESDLHTDADRVADIKNWLSAVISYQQSCLDGLGEFDPQLKQRMQ 180
           LQ AIDEL ASYS VG+ DLHT+ DR+ADIKNWL+AVISYQQSCLDGL EFDPQLKQ+MQ
Sbjct: 121 LQSAIDELHASYSTVGDPDLHTNEDRIADIKNWLTAVISYQQSCLDGLEEFDPQLKQKMQ 180

Query: 181 DGLDVAGKLTSNALAIVTAVSNILDNYRLQLKVQPSGRRLLGTTVVDRDGFPTWLTGADR 240
           DGLDVAGKLTSNALAIV AVS+IL    LQLKVQPSGRRLLGTT VD D FPTWLTG+DR
Sbjct: 181 DGLDVAGKLTSNALAIVGAVSDILKELGLQLKVQPSGRRLLGTTEVDSDSFPTWLTGSDR 240

Query: 241 KLLASKQGGVRPTPNAVVAKDGSGKYKTIAAALAAYPKVLRGRYVIYVKAGIYDEYITLT 300
           KLLA+K+GGVR  PN VVAKDGSG+YKTI AALAAYPK L+GRYVIYVKAG+Y+E I +T
Sbjct: 241 KLLAAKRGGVRVKPNVVVAKDGSGQYKTIGAALAAYPKALKGRYVIYVKAGVYNEPIIVT 300

Query: 301 KDMKNVFMYGDGPRKTIVTGRKSNRDGFTTQNTASFAAIGEGFLCKSMGFTNTAGPEGHQ 360
           KDMKN+FMYGDGPRKTIVTGRKSNRDG TTQNTASFAAIGEGFLCKSMGFTNTAGPEGHQ
Sbjct: 301 KDMKNIFMYGDGPRKTIVTGRKSNRDGITTQNTASFAAIGEGFLCKSMGFTNTAGPEGHQ 360

Query: 361 AVALRVQSDRSAFFNCRMDGHQDTLYVQTHRQFYRNCVVSGTVDFIFGDSSTVIQNSLII 420
           AVALRVQSDRSAFFNCRMDG+QDTLYVQTHRQFYRNCV+SGTVDFIFGDS+T+IQNSLII
Sbjct: 361 AVALRVQSDRSAFFNCRMDGYQDTLYVQTHRQFYRNCVISGTVDFIFGDSNTIIQNSLII 420

Query: 421 VRRPMDNQQNTVTAQGRAEQKEITGLVIHNCRIVPEQKLFAERFKIPTFLGRPWKQYART 480
           VRRPMDNQQNTVTA GRA++KEI+GLVIHNCRIVPEQKLFAERFKIPTFLGRPWK+YART
Sbjct: 421 VRRPMDNQQNTVTANGRADKKEISGLVIHNCRIVPEQKLFAERFKIPTFLGRPWKEYART 480

Query: 481 VIMESTLGDFIQPAGYMPWSGDFALETCLYLEYGNRGPGANTNRRVRWKGAKVIGRNEAL 540
           VIMESTLGDFIQPAGYMPWSG+FAL TC Y EYGNRGPGANTNRRVRWKG +VIGRNEA+
Sbjct: 481 VIMESTLGDFIQPAGYMPWSGNFALATCSYFEYGNRGPGANTNRRVRWKGVRVIGRNEAM 540

Query: 541 QYTAGAFLLGRSWLPTTGGLYYLGLKN 568
           Q+TAG FLLG++WLP TGG Y LGLKN
Sbjct: 541 QFTAGPFLLGKAWLPGTGGPYLLGLKN 566

[Close]

BLAST of CSPI05G02400 vs. ExPASy TrEMBL
Match: A0A5A7U7W4 (Pectinesterase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold180G00090 PE=3 SV=1)

HSP 1 Score: 932.2 bits (2408), Expect = 1.0e-267
Identity = 463/567 (81.66%), Postives = 514/567 (90.65%), Query Frame = 0


Query: 1   MLGKVIVSGLSLILVVGIALAIAATINKSNASNGGTEDVSPKMKAVSAICSTTDYQDECK 60
           M+ KV+++  SL LVVG+ LA+ A +NK N S+  T D+SPKMKAVS+ICS TDYQ EC+
Sbjct: 1   MVAKVVMAVTSL-LVVGVVLAVFAMVNK-NHSSDETADMSPKMKAVSSICSNTDYQQECQ 60

Query: 61  TTLDHVARNTSSNDPKDYAEAAILATIGEITKGYNLSDSLIVEASTNASIKMSVEDCKDL 120
           TTL +V  N+S +DPK++ +AAI ATI E+ KGYNL+D+L+VEA+ NASIKM+V+DC+DL
Sbjct: 61  TTLGNVGHNSSVDDPKEFLKAAITATIEEMKKGYNLTDNLMVEAANNASIKMTVDDCRDL 120

Query: 121 LQFAIDELQASYSAVGESDLHTDADRVADIKNWLSAVISYQQSCLDGLGEFDPQLKQRMQ 180
           LQ AIDEL ASYS VG+ DLHT+ DRVADIKNWL+AVISYQQSCLDGL EFDPQLKQ+MQ
Sbjct: 121 LQSAIDELHASYSTVGDPDLHTNEDRVADIKNWLTAVISYQQSCLDGLEEFDPQLKQKMQ 180

Query: 181 DGLDVAGKLTSNALAIVTAVSNILDNYRLQLKVQPSGRRLLGTTVVDRDGFPTWLTGADR 240
            GLDVA KLTSNALAIV AVS+IL +  LQLKVQPSGRRLLGTT VD DG+PTWLTGADR
Sbjct: 181 GGLDVAAKLTSNALAIVDAVSDILKSLGLQLKVQPSGRRLLGTTEVDNDGYPTWLTGADR 240

Query: 241 KLLASKQGGVRPTPNAVVAKDGSGKYKTIAAALAAYPKVLRGRYVIYVKAGIYDEYITLT 300
           KLLA+K+GGVR  PNAVVAKDGSG+YKTIAAALAAYPK L+GRYVIYVKAGIY+EYI +T
Sbjct: 241 KLLAAKRGGVRVKPNAVVAKDGSGQYKTIAAALAAYPKALKGRYVIYVKAGIYNEYIIVT 300

Query: 301 KDMKNVFMYGDGPRKTIVTGRKSNRDGFTTQNTASFAAIGEGFLCKSMGFTNTAGPEGHQ 360
           KDMKNVFMYGDGPRKTIVTGRKSNRDG  TQNTA+FAAIGEGFLCKSMGFTNTAGPEGHQ
Sbjct: 301 KDMKNVFMYGDGPRKTIVTGRKSNRDGINTQNTATFAAIGEGFLCKSMGFTNTAGPEGHQ 360

Query: 361 AVALRVQSDRSAFFNCRMDGHQDTLYVQTHRQFYRNCVVSGTVDFIFGDSSTVIQNSLII 420
           AVALRVQSDRSAFFNCRMDG+QDTLYVQTHRQFYRNCVVSGTVDFIFGDS+T+IQNSLII
Sbjct: 361 AVALRVQSDRSAFFNCRMDGYQDTLYVQTHRQFYRNCVVSGTVDFIFGDSNTIIQNSLII 420

Query: 421 VRRPMDNQQNTVTAQGRAEQKEITGLVIHNCRIVPEQKLFAERFKIPTFLGRPWKQYART 480
           VRRPMDNQQNTVTA GRA++KEI+GLVIHNCRIVPEQKLFAERFKIPTFLGRPWK+YART
Sbjct: 421 VRRPMDNQQNTVTANGRADKKEISGLVIHNCRIVPEQKLFAERFKIPTFLGRPWKEYART 480

Query: 481 VIMESTLGDFIQPAGYMPWSGDFALETCLYLEYGNRGPGANTNRRVRWKGAKVIGRNEAL 540
           VIME+TLGDFIQPAGYMPWSGDFAL TC Y EYGNRGPGANT RRVRWKG +VIGRNEA+
Sbjct: 481 VIMETTLGDFIQPAGYMPWSGDFALATCSYFEYGNRGPGANTKRRVRWKGVRVIGRNEAM 540

Query: 541 QYTAGAFLLGRSWLPTTGGLYYLGLKN 568
           Q+TAG FLLGR+WLP TGG Y LGLKN
Sbjct: 541 QFTAGPFLLGRAWLPATGGPYLLGLKN 565

[Close]

BLAST of CSPI05G02400 vs. NCBI nr
Match: XP_004149499.1 (pectinesterase [Cucumis sativus] >KGN49780.1 hypothetical protein Csa_004678 [Cucumis sativus])

HSP 1 Score: 1122.8 bits (2903), Expect = 0.0e+00
Identity = 565/567 (99.65%), Postives = 566/567 (99.82%), Query Frame = 0


Query: 1   MLGKVIVSGLSLILVVGIALAIAATINKSNASNGGTEDVSPKMKAVSAICSTTDYQDECK 60
           MLGKVIVSGLSLILVVGIALAIAATINKSNASNGGTEDVSPKMKAVSAICSTTDYQDECK
Sbjct: 1   MLGKVIVSGLSLILVVGIALAIAATINKSNASNGGTEDVSPKMKAVSAICSTTDYQDECK 60

Query: 61  TTLDHVARNTSSNDPKDYAEAAILATIGEITKGYNLSDSLIVEASTNASIKMSVEDCKDL 120
           TTLDHVARNTSSNDPKDYAEAAILATIGEITKGYNLSDSLIVEASTNASIKMSVEDCKDL
Sbjct: 61  TTLDHVARNTSSNDPKDYAEAAILATIGEITKGYNLSDSLIVEASTNASIKMSVEDCKDL 120

Query: 121 LQFAIDELQASYSAVGESDLHTDADRVADIKNWLSAVISYQQSCLDGLGEFDPQLKQRMQ 180
           LQFAIDELQASYSAVGESDLHTD+DRVADIKNWLSAVISYQQSCLDGLGEFDPQLKQRMQ
Sbjct: 121 LQFAIDELQASYSAVGESDLHTDSDRVADIKNWLSAVISYQQSCLDGLGEFDPQLKQRMQ 180

Query: 181 DGLDVAGKLTSNALAIVTAVSNILDNYRLQLKVQPSGRRLLGTTVVDRDGFPTWLTGADR 240
           DGLDVAGKLTSNALAIVTAVSNILDNYRLQLKVQPSGRRLLGTTVVDRDGFPTWLTGADR
Sbjct: 181 DGLDVAGKLTSNALAIVTAVSNILDNYRLQLKVQPSGRRLLGTTVVDRDGFPTWLTGADR 240

Query: 241 KLLASKQGGVRPTPNAVVAKDGSGKYKTIAAALAAYPKVLRGRYVIYVKAGIYDEYITLT 300
           KLLASKQ GVRPTPNAVVAKDGSGKYKTIAAALAAYPKVLRGRYVIYVKAGIYDEYITLT
Sbjct: 241 KLLASKQRGVRPTPNAVVAKDGSGKYKTIAAALAAYPKVLRGRYVIYVKAGIYDEYITLT 300

Query: 301 KDMKNVFMYGDGPRKTIVTGRKSNRDGFTTQNTASFAAIGEGFLCKSMGFTNTAGPEGHQ 360
           KDMKNVFMYGDGPRKTIVTGRKSNRDGFTTQNTASFAAIGEGFLCKSMGFTNTAGPEGHQ
Sbjct: 301 KDMKNVFMYGDGPRKTIVTGRKSNRDGFTTQNTASFAAIGEGFLCKSMGFTNTAGPEGHQ 360

Query: 361 AVALRVQSDRSAFFNCRMDGHQDTLYVQTHRQFYRNCVVSGTVDFIFGDSSTVIQNSLII 420
           AVALRVQSDRSAFFNCRMDGHQDTLYVQTHRQFYRNCVVSGTVDFIFGDSSTVIQNSLII
Sbjct: 361 AVALRVQSDRSAFFNCRMDGHQDTLYVQTHRQFYRNCVVSGTVDFIFGDSSTVIQNSLII 420

Query: 421 VRRPMDNQQNTVTAQGRAEQKEITGLVIHNCRIVPEQKLFAERFKIPTFLGRPWKQYART 480
           VRRPMDNQQNTVTAQGRAEQKEITGLVIHNCRIVPEQKLFAERFKIPTFLGRPWKQYART
Sbjct: 421 VRRPMDNQQNTVTAQGRAEQKEITGLVIHNCRIVPEQKLFAERFKIPTFLGRPWKQYART 480

Query: 481 VIMESTLGDFIQPAGYMPWSGDFALETCLYLEYGNRGPGANTNRRVRWKGAKVIGRNEAL 540
           VIMESTLGDFIQPAGYMPWSGDFALETCLYLEYGNRGPGANTNRRVRWKGAKVIGRNEAL
Sbjct: 481 VIMESTLGDFIQPAGYMPWSGDFALETCLYLEYGNRGPGANTNRRVRWKGAKVIGRNEAL 540

Query: 541 QYTAGAFLLGRSWLPTTGGLYYLGLKN 568
           QYTAGAFLLGRSWLPTTGGLYYLGLKN
Sbjct: 541 QYTAGAFLLGRSWLPTTGGLYYLGLKN 567

[Close]

BLAST of CSPI05G02400 vs. NCBI nr
Match: KAA0050274.1 (pectinesterase-like [Cucumis melo var. makuwa] >TYJ98133.1 pectinesterase-like [Cucumis melo var. makuwa])

HSP 1 Score: 1075.1 bits (2779), Expect = 0.0e+00
Identity = 533/567 (94.00%), Postives = 550/567 (97.00%), Query Frame = 0


Query: 1   MLGKVIVSGLSLILVVGIALAIAATINKSNASNGGTEDVSPKMKAVSAICSTTDYQDECK 60
           M+GKVIVS LSLILVVGIALA+ ATINK N SNGG E+VSPKMKA+SAICSTTDYQ+ECK
Sbjct: 1   MVGKVIVSALSLILVVGIALAVVATINKRNGSNGGKEEVSPKMKAISAICSTTDYQEECK 60

Query: 61  TTLDHVARNTSSNDPKDYAEAAILATIGEITKGYNLSDSLIVEASTNASIKMSVEDCKDL 120
            TL+HVA NTSSNDPKDYA+AAILATI EITKGYNLSDSLIVEA  NASIKMSVEDCKDL
Sbjct: 61  NTLNHVAHNTSSNDPKDYAKAAILATIKEITKGYNLSDSLIVEAGNNASIKMSVEDCKDL 120

Query: 121 LQFAIDELQASYSAVGESDLHTDADRVADIKNWLSAVISYQQSCLDGLGEFDPQLKQRMQ 180
           LQFAIDELQASYS VGESDLHTDADR+ADIKNWLSAV+SYQQSCLDGLGEFDPQLKQRMQ
Sbjct: 121 LQFAIDELQASYSTVGESDLHTDADRIADIKNWLSAVVSYQQSCLDGLGEFDPQLKQRMQ 180

Query: 181 DGLDVAGKLTSNALAIVTAVSNILDNYRLQLKVQPSGRRLLGTTVVDRDGFPTWLTGADR 240
           DGLDVAGKLTSNALAIVTAVSNILDNY LQLKVQPSGRRLLGTT VD DGFPTWLTGADR
Sbjct: 181 DGLDVAGKLTSNALAIVTAVSNILDNYGLQLKVQPSGRRLLGTTEVDSDGFPTWLTGADR 240

Query: 241 KLLASKQGGVRPTPNAVVAKDGSGKYKTIAAALAAYPKVLRGRYVIYVKAGIYDEYITLT 300
           KLLASKQGGVRPTPNAVVAKDGSG+YKTIAAALAAYPK LRGRYVIYVKAGIYDEYIT+T
Sbjct: 241 KLLASKQGGVRPTPNAVVAKDGSGQYKTIAAALAAYPKALRGRYVIYVKAGIYDEYITVT 300

Query: 301 KDMKNVFMYGDGPRKTIVTGRKSNRDGFTTQNTASFAAIGEGFLCKSMGFTNTAGPEGHQ 360
           KDMKNVFMYGDGPRKTIVTGRKSNRDGFTTQNTASFAAIGEGFLCKSMGFTNTAGP+GHQ
Sbjct: 301 KDMKNVFMYGDGPRKTIVTGRKSNRDGFTTQNTASFAAIGEGFLCKSMGFTNTAGPQGHQ 360

Query: 361 AVALRVQSDRSAFFNCRMDGHQDTLYVQTHRQFYRNCVVSGTVDFIFGDSSTVIQNSLII 420
           AVALRVQSDRSAFFNCRMDGHQDTLYVQTHRQFYRNCV+SGTVDFIFGDSST+IQNSLII
Sbjct: 361 AVALRVQSDRSAFFNCRMDGHQDTLYVQTHRQFYRNCVISGTVDFIFGDSSTIIQNSLII 420

Query: 421 VRRPMDNQQNTVTAQGRAEQKEITGLVIHNCRIVPEQKLFAERFKIPTFLGRPWKQYART 480
           VRRPMDNQQNTVTAQGRAEQKEITGLVIHNCRIVPEQKLFAERFKIPTFLGRPWKQYART
Sbjct: 421 VRRPMDNQQNTVTAQGRAEQKEITGLVIHNCRIVPEQKLFAERFKIPTFLGRPWKQYART 480

Query: 481 VIMESTLGDFIQPAGYMPWSGDFALETCLYLEYGNRGPGANTNRRVRWKGAKVIGRNEAL 540
           VIMESTLGDFIQPAGYMPWSG+FALETCLYLEYGNRGPGANTNRRVRWKG KVIGRNEAL
Sbjct: 481 VIMESTLGDFIQPAGYMPWSGNFALETCLYLEYGNRGPGANTNRRVRWKGVKVIGRNEAL 540

Query: 541 QYTAGAFLLGRSWLPTTGGLYYLGLKN 568
           QYTAGAFLLGR+WLP TGG+YYLGLKN
Sbjct: 541 QYTAGAFLLGRTWLPRTGGIYYLGLKN 567

[Close]

BLAST of CSPI05G02400 vs. NCBI nr
Match: XP_008466403.1 (PREDICTED: pectinesterase-like [Cucumis melo])

HSP 1 Score: 1072.4 bits (2772), Expect = 1.3e-309
Identity = 531/567 (93.65%), Postives = 550/567 (97.00%), Query Frame = 0


Query: 1   MLGKVIVSGLSLILVVGIALAIAATINKSNASNGGTEDVSPKMKAVSAICSTTDYQDECK 60
           M+GKVIVS LSLILVVGIALA+ ATINK N SNGG E+VSPKMKA+SAICSTTDYQ+ECK
Sbjct: 1   MVGKVIVSALSLILVVGIALAVVATINKRNGSNGGKEEVSPKMKAISAICSTTDYQEECK 60

Query: 61  TTLDHVARNTSSNDPKDYAEAAILATIGEITKGYNLSDSLIVEASTNASIKMSVEDCKDL 120
            TL+HVA+NTSSNDPKDYA+AAILATI EITKGYNLSDSLIVEA  NASIKMSVEDC DL
Sbjct: 61  NTLNHVAQNTSSNDPKDYAKAAILATIKEITKGYNLSDSLIVEAGNNASIKMSVEDCNDL 120

Query: 121 LQFAIDELQASYSAVGESDLHTDADRVADIKNWLSAVISYQQSCLDGLGEFDPQLKQRMQ 180
           LQFAIDELQASYS VGESDLHTDADR+ADIKNWLSAV+SYQQSCLDGLGEFDPQLKQRMQ
Sbjct: 121 LQFAIDELQASYSTVGESDLHTDADRIADIKNWLSAVVSYQQSCLDGLGEFDPQLKQRMQ 180

Query: 181 DGLDVAGKLTSNALAIVTAVSNILDNYRLQLKVQPSGRRLLGTTVVDRDGFPTWLTGADR 240
           DGLDVAGKLTSNALAI+TAVSNILDNY LQLKVQPSGRRLLGTT VD DGFPTWLTGADR
Sbjct: 181 DGLDVAGKLTSNALAILTAVSNILDNYGLQLKVQPSGRRLLGTTEVDSDGFPTWLTGADR 240

Query: 241 KLLASKQGGVRPTPNAVVAKDGSGKYKTIAAALAAYPKVLRGRYVIYVKAGIYDEYITLT 300
           KLLASKQGGVRPTPNAVVAKDGSG+YKTIAAALAAYPK LRGRYVIYVKAGIYDEYIT+T
Sbjct: 241 KLLASKQGGVRPTPNAVVAKDGSGQYKTIAAALAAYPKALRGRYVIYVKAGIYDEYITVT 300

Query: 301 KDMKNVFMYGDGPRKTIVTGRKSNRDGFTTQNTASFAAIGEGFLCKSMGFTNTAGPEGHQ 360
           KDMKNVFMYGDGPRKTIVTGRKSNRDGFTTQNTASFAAIGEGFLCKSMGFTNTAGP+GHQ
Sbjct: 301 KDMKNVFMYGDGPRKTIVTGRKSNRDGFTTQNTASFAAIGEGFLCKSMGFTNTAGPQGHQ 360

Query: 361 AVALRVQSDRSAFFNCRMDGHQDTLYVQTHRQFYRNCVVSGTVDFIFGDSSTVIQNSLII 420
           AVALRVQSDRSAFFNCRMDGHQDTLYVQTHRQFYRNCV+SGTVDFIFGDSST+IQNSLII
Sbjct: 361 AVALRVQSDRSAFFNCRMDGHQDTLYVQTHRQFYRNCVISGTVDFIFGDSSTIIQNSLII 420

Query: 421 VRRPMDNQQNTVTAQGRAEQKEITGLVIHNCRIVPEQKLFAERFKIPTFLGRPWKQYART 480
           VRRPMDNQQNTVTAQGRAEQKEITGLVIHNCRIVPEQKLFAERFKIPTFLGRPWKQYART
Sbjct: 421 VRRPMDNQQNTVTAQGRAEQKEITGLVIHNCRIVPEQKLFAERFKIPTFLGRPWKQYART 480

Query: 481 VIMESTLGDFIQPAGYMPWSGDFALETCLYLEYGNRGPGANTNRRVRWKGAKVIGRNEAL 540
           VIMESTLGDFIQPAGYMPWSG+FALETCLYLEYGNRGPGANTNRRVRWKG KVIGRNEAL
Sbjct: 481 VIMESTLGDFIQPAGYMPWSGNFALETCLYLEYGNRGPGANTNRRVRWKGVKVIGRNEAL 540

Query: 541 QYTAGAFLLGRSWLPTTGGLYYLGLKN 568
           QYTAGAFLLGR+WLP TGG+YYLGLKN
Sbjct: 541 QYTAGAFLLGRTWLPRTGGIYYLGLKN 567

[Close]

BLAST of CSPI05G02400 vs. NCBI nr
Match: XP_038875889.1 (pectinesterase-like [Benincasa hispida])

HSP 1 Score: 979.5 bits (2531), Expect = 1.2e-281
Identity = 484/568 (85.21%), Postives = 527/568 (92.78%), Query Frame = 0


Query: 1   MLGKVIVSGLSLILVVGIALAIAATINKSNASNGGTEDVSPKMKAVSAICSTTDYQDECK 60
           M+GKV+VSGLSLILVVG+ALA+ AT+NKSN+SN   E  SPKMKA+S ICST+DYQ+ECK
Sbjct: 1   MVGKVVVSGLSLILVVGVALAVVATVNKSNSSNAALE--SPKMKAISTICSTSDYQEECK 60

Query: 61  TTLDHVARNTSSNDPKDYAEAAILATIGEITKGYNLSDSLIVEASTNASIKMSVEDCKDL 120
            TL +VA N +SNDPKDYA+AA+LATI EITKGYN SD+L+VEA+ NAS KMSVEDCKDL
Sbjct: 61  NTLSNVAHNATSNDPKDYAKAAVLATIEEITKGYNFSDTLMVEAANNASTKMSVEDCKDL 120

Query: 121 LQFAIDELQASYSAVGESDLHTDADRVADIKNWLSAVISYQQSCLDGLGEFDPQLKQRMQ 180
           LQFAIDELQ SYSA+G+SDLHTD DRVADI NWLSAVISYQQSCLDGL EF+PQLKQ+MQ
Sbjct: 121 LQFAIDELQESYSAIGDSDLHTDTDRVADINNWLSAVISYQQSCLDGLEEFNPQLKQKMQ 180

Query: 181 DGLDVAGKLTSNALAIVTAVSNILDNYRLQLK-VQPSGRRLLGTTVVDRDGFPTWLTGAD 240
           D L VA KLTSNALA+V+AVSNILDNYRLQLK VQP+GRRLLGTT V  DGFP WL+GAD
Sbjct: 181 DNLSVASKLTSNALAVVSAVSNILDNYRLQLKQVQPNGRRLLGTTEVGSDGFPIWLSGAD 240

Query: 241 RKLLASKQGGVRPTPNAVVAKDGSGKYKTIAAALAAYPKVLRGRYVIYVKAGIYDEYITL 300
           RKLLA++  G R  PNAVVAKDGSG+YK+IAAALAAYPK L+GRYVIYVKAGIYDE+I +
Sbjct: 241 RKLLAARGRGGRARPNAVVAKDGSGQYKSIAAALAAYPKALKGRYVIYVKAGIYDEHIIV 300

Query: 301 TKDMKNVFMYGDGPRKTIVTGRKSNRDGFTTQNTASFAAIGEGFLCKSMGFTNTAGPEGH 360
           TKDMKNVFMYGDGPRKTIVTGRK+NRDGFTTQNTASFAA+G GFLCKSMGFTNTAGPEGH
Sbjct: 301 TKDMKNVFMYGDGPRKTIVTGRKNNRDGFTTQNTASFAALGNGFLCKSMGFTNTAGPEGH 360

Query: 361 QAVALRVQSDRSAFFNCRMDGHQDTLYVQTHRQFYRNCVVSGTVDFIFGDSSTVIQNSLI 420
           QAVALRVQSDRSAFFNCRMDGHQDTLY QTHRQFYRNCV+SGTVDFIFGDS+T+IQNSLI
Sbjct: 361 QAVALRVQSDRSAFFNCRMDGHQDTLYAQTHRQFYRNCVISGTVDFIFGDSTTLIQNSLI 420

Query: 421 IVRRPMDNQQNTVTAQGRAEQKEITGLVIHNCRIVPEQKLFAERFKIPTFLGRPWKQYAR 480
           IVRRPMDNQQNTVTAQGR  QK+ITGLVIHNCRIVPEQKLFAERFKIPTFLGRPWKQYAR
Sbjct: 421 IVRRPMDNQQNTVTAQGRDLQKQITGLVIHNCRIVPEQKLFAERFKIPTFLGRPWKQYAR 480

Query: 481 TVIMESTLGDFIQPAGYMPWSGDFALETCLYLEYGNRGPGANTNRRVRWKGAKVIGRNEA 540
           TVIME+TLGDFIQPAGYMPWSG+FALETCLYLEYGNRGPGANTNRRVRWKG +VIGRNEA
Sbjct: 481 TVIMETTLGDFIQPAGYMPWSGNFALETCLYLEYGNRGPGANTNRRVRWKGVRVIGRNEA 540

Query: 541 LQYTAGAFLLGRSWLPTTGGLYYLGLKN 568
           LQYTAG FLLGR+WLP+TGG Y+LGLKN
Sbjct: 541 LQYTAGPFLLGRAWLPSTGGPYFLGLKN 566

[Close]

BLAST of CSPI05G02400 vs. NCBI nr
Match: XP_038875879.1 (LOW QUALITY PROTEIN: pectinesterase-like [Benincasa hispida])

HSP 1 Score: 949.9 bits (2454), Expect = 1.0e-272
Identity = 469/567 (82.72%), Postives = 517/567 (91.18%), Query Frame = 0


Query: 1   MLGKVIVSGLSLILVVGIALAIAATINKSNASNGGTEDVSPKMKAVSAICSTTDYQDECK 60
           M+GKV+VSG+SLILVVG+ALA+ A + K+N  N   +  SPKMKAVS ICSTTDYQ EC+
Sbjct: 1   MVGKVVVSGISLILVVGVALAVVAMVTKNN--NSDVDLSSPKMKAVSTICSTTDYQQECQ 60

Query: 61  TTLDHVARNTSSNDPKDYAEAAILATIGEITKGYNLSDSLIVEASTNASIKMSVEDCKDL 120
            TL +VA+N S +DPK+YA+AA+LATI EITKGYNL+  L+VEA+ NASIKMSV+DCKDL
Sbjct: 61  NTLTNVAQNASVDDPKEYAKAAVLATIQEITKGYNLTADLMVEAANNASIKMSVDDCKDL 120

Query: 121 LQFAIDELQASYSAVGESDLHTDADRVADIKNWLSAVISYQQSCLDGLGEFDPQLKQRMQ 180
           LQ AIDEL ASYS VG+ DLHT+ DRVADIKNWL+AVISYQQSCLDGL EFDPQLKQ+MQ
Sbjct: 121 LQSAIDELHASYSTVGDPDLHTNEDRVADIKNWLTAVISYQQSCLDGLEEFDPQLKQKMQ 180

Query: 181 DGLDVAGKLTSNALAIVTAVSNILDNYRLQLKVQPSGRRLLGTTVVDRDGFPTWLTGADR 240
           DGLDVAGKLTSNALAIV AVS+IL N+ LQLKVQPSGRRLL TT +  DGFPTW++GADR
Sbjct: 181 DGLDVAGKLTSNALAIVGAVSDILKNFGLQLKVQPSGRRLLETTEIGSDGFPTWMSGADR 240

Query: 241 KLLASKQGGVRPTPNAVVAKDGSGKYKTIAAALAAYPKVLRGRYVIYVKAGIYDEYITLT 300
           KLLAS+ GG R  PNAVVAKDGSG+YK+I AALAAYPK LRGRYVIYVKAGIYDEYIT+T
Sbjct: 241 KLLASRGGGARVKPNAVVAKDGSGQYKSIGAALAAYPKALRGRYVIYVKAGIYDEYITVT 300

Query: 301 KDMKNVFMYGDGPRKTIVTGRKSNRDGFTTQNTASFAAIGEGFLCKSMGFTNTAGPEGHQ 360
           KDMKNVFMYGDGPRKTIVTGRK+NRDG TTQNTASF+AIGEGFLCKSMGF NTAGPEGHQ
Sbjct: 301 KDMKNVFMYGDGPRKTIVTGRKNNRDGITTQNTASFSAIGEGFLCKSMGFRNTAGPEGHQ 360

Query: 361 AVALRVQSDRSAFFNCRMDGHQDTLYVQTHRQFYRNCVVSGTVDFIFGDSSTVIQNSLII 420
           AVALRVQSDRSAF+NCRMDG+QDTLYVQTHRQFYRNCV+SGTVDFIFGDSST+IQNSLII
Sbjct: 361 AVALRVQSDRSAFYNCRMDGYQDTLYVQTHRQFYRNCVISGTVDFIFGDSSTIIQNSLII 420

Query: 421 VRRPMDNQQNTVTAQGRAEQKEITGLVIHNCRIVPEQKLFAERFKIPTFLGRPWKQYART 480
           VRRPMDNQ+NTVTA GRA++KEI+GLVIHNCRIVPEQKLFAERFKIPTFLGRPWKQYART
Sbjct: 421 VRRPMDNQKNTVTANGRADKKEISGLVIHNCRIVPEQKLFAERFKIPTFLGRPWKQYART 480

Query: 481 VIMESTLGDFIQPAGYMPWSGDFALETCLYLEYGNRGPGANTNRRVRWKGAKVIGRNEAL 540
           VIME+TLGDFIQP GYMPWSG+FALETC Y EYGNRGPGANTNRRVRWKG +VIGRNEAL
Sbjct: 481 VIMETTLGDFIQPDGYMPWSGNFALETCSYFEYGNRGPGANTNRRVRWKGVRVIGRNEAL 540

Query: 541 QYTAGAFLLGRSWLPTTGGLYYLGLKN 568
           QYTAG FL+GR+WLP TGG Y LGLK+
Sbjct: 541 QYTAGPFLMGRAWLPATGGPYLLGLKH 565

[Close]

BLAST of CSPI05G02400 vs. TAIR 10
Match: AT5G27870.1 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 444.9 bits (1143), Expect = 9.8e-125
Identity = 221/562 (39.32%), Postives = 348/562 (61.92%), Query Frame = 0


Query: 4   KVIVSGLSLILVVGIALAIAATINKSNASNGGTEDVSPKMKAVSAICSTTDYQDECKTTL 63
           + ++  +S +L++ + +A+   ++ + + N G E+++  +KA+  +C+ TDY++ C+ TL
Sbjct: 15  RYVIISISSVLLISMVVAVTIGVSVNKSDNAGDEEITTSVKAIKDVCAPTDYKETCEDTL 74

Query: 64  DHVARNTSSNDPKDYAEAAILATIGEITKGYNLSDSLIVEASTNASIKMSVEDCKDLLQF 123
              A++TS  DP +  + A  AT+ +I+     S ++I E   +   KM+++ CK+L+ +
Sbjct: 75  RKDAKDTS--DPLELVKTAFNATMKQISDVAKKSQTMI-ELQKDPRAKMALDQCKELMDY 134

Query: 124 AIDELQASYSAVGESDLHTDADRVADIKNWLSAVISYQQSCLDGLGEFDPQLKQRMQDGL 183
           AI EL  S+  +G+ + H   + +  ++ WLSA IS++Q+CLDG         + ++  L
Sbjct: 135 AIGELSKSFEELGKFEFHKVDEALVKLRIWLSATISHEQTCLDGFQGTQGNAGETIKKAL 194

Query: 184 DVAGKLTSNALAIVTAVSNILDNYRLQLKVQPSGRRLLGTTVVDRDGFPTWLTGADRKLL 243
             A +LT N LA+VT +SN L   ++    + + RRLL         FP+W+    R+LL
Sbjct: 195 KTAVQLTHNGLAMVTEMSNYLGQMQIP---EMNSRRLLS------QEFPSWMDARARRLL 254

Query: 244 ASKQGGVRPTPNAVVAKDGSGKYKTIAAALAAYPKVLRGRYVIYVKAGIYDEYITLTKDM 303
            +    V+  P+ VVA+DGSG+YKTI  AL   PK     +V+++K GIY EY+ + + M
Sbjct: 255 NAPMSEVK--PDIVVAQDGSGQYKTINEALNFVPKKKNTTFVVHIKEGIYKEYVQVNRSM 314

Query: 304 KNVFMYGDGPRKTIVTGRKSNRDGFTTQNTASFAAIGEGFLCKSMGFTNTAGPEGHQAVA 363
            ++   GDGP KT+++G KS +DG TT  TA+ A +G+ F+ K++ F NTAG   HQAVA
Sbjct: 315 THLVFIGDGPDKTVISGSKSYKDGITTYKTATVAIVGDHFIAKNIAFENTAGAIKHQAVA 374

Query: 364 LRVQSDRSAFFNCRMDGHQDTLYVQTHRQFYRNCVVSGTVDFIFGDSSTVIQNSLIIVRR 423
           +RV +D S F+NC+ DG+QDTLY  +HRQFYR+C +SGT+DF+FGD++ V QN  ++VR+
Sbjct: 375 IRVLADESIFYNCKFDGYQDTLYAHSHRQFYRDCTISGTIDFLFGDAAAVFQNCTLLVRK 434

Query: 424 PMDNQQNTVTAQGRAEQKEITGLVIHNCRIVPEQKLFAERFKIPTFLGRPWKQYARTVIM 483
           P+ NQ   +TA GR + +E TG V+  C IV E    A + +  T+LGRPWK+Y+RT+IM
Sbjct: 435 PLLNQACPITAHGRKDPRESTGFVLQGCTIVGEPDYLAVKEQSKTYLGRPWKEYSRTIIM 494

Query: 484 ESTLGDFIQPAGYMPWSGDFALETCLYLEYGNRGPGANTNRRVRWKGAKVIGRNEALQYT 543
            + + DF+ P G+ PW G+F L T  Y E  N GPGA   +RV W G K +   E L++T
Sbjct: 495 NTFIPDFVPPEGWQPWLGEFGLNTLFYSEVQNTGPGAAITKRVTWPGIKKLSDEEILKFT 554

Query: 544 AGAFLLGRSWLPTTGGLYYLGL 566
              ++ G +W+P  G  Y LGL
Sbjct: 555 PAQYIQGDAWIPGKGVPYILGL 562

[Close]

BLAST of CSPI05G02400 vs. TAIR 10
Match: AT3G05610.1 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 442.6 bits (1137), Expect = 4.8e-124
Identity = 223/561 (39.75%), Postives = 343/561 (61.14%), Query Frame = 0


Query: 5   VIVSGLSLILVVGIALAIAATINKSNASNGGTEDVSPKMKAVSAICSTTDYQDECKTTLD 64
           + +S + LI +V +A+ +  ++NK +  + G  +V+  +KAV  +C+ TDY+  C+ TL 
Sbjct: 18  ITISSVLLISMV-VAVTVGVSLNKHDGDSKGKAEVNASVKAVKDVCAPTDYRKTCEDTLI 77

Query: 65  HVARNTSSNDPKDYAEAAILATIGEITKGYNLSDSLIVEASTNASIKMSVEDCKDLLQFA 124
              +NT+  DP +  + A   T+ +IT     S + I+E   ++  +M+++ CK+L+ +A
Sbjct: 78  KNGKNTT--DPMELVKTAFNVTMKQITDAAKKSQT-IMELQKDSRTRMALDQCKELMDYA 137

Query: 125 IDELQASYSAVGESDLHTDADRVADIKNWLSAVISYQQSCLDGLGEFDPQLKQRMQDGLD 184
           +DEL  S+  +G+ + H   + + +++ WLSA IS++++CL+G         + M+  L 
Sbjct: 138 LDELSNSFEELGKFEFHLLDEALINLRIWLSAAISHEETCLEGFQGTQGNAGETMKKALK 197

Query: 185 VAGKLTSNALAIVTAVSNILDNYRLQLKVQPSGRRLLGTTVVDRDGFPTWLTGADRKLLA 244
            A +LT N LAI++ +SN +   ++      + RRLL       +GFP+W+    RKLL 
Sbjct: 198 TAIELTHNGLAIISEMSNFVGQMQIP---GLNSRRLLA------EGFPSWVDQRGRKLLQ 257

Query: 245 SKQGGVRPTPNAVVAKDGSGKYKTIAAALAAYPKVLRGRYVIYVKAGIYDEYITLTKDMK 304
           +        P+ VVA+DGSG+YKTI  AL   PK     +V+++KAG+Y EY+ + K M 
Sbjct: 258 AAAAYSDVKPDIVVAQDGSGQYKTINEALQFVPKKRNTTFVVHIKAGLYKEYVQVNKTMS 317

Query: 305 NVFMYGDGPRKTIVTGRKSNRDGFTTQNTASFAAIGEGFLCKSMGFTNTAGPEGHQAVAL 364
           ++   GDGP KTI++G K+ +DG TT  TA+ A +G  F+ K++GF NTAG   HQAVA+
Sbjct: 318 HLVFIGDGPDKTIISGNKNYKDGITTYRTATVAIVGNYFIAKNIGFENTAGAIKHQAVAV 377

Query: 365 RVQSDRSAFFNCRMDGHQDTLYVQTHRQFYRNCVVSGTVDFIFGDSSTVIQNSLIIVRRP 424
           RVQSD S FFNCR DG+QDTLY  +HRQF+R+C +SGT+DF+FGD++ V QN  ++VR+P
Sbjct: 378 RVQSDESIFFNCRFDGYQDTLYTHSHRQFFRDCTISGTIDFLFGDAAAVFQNCTLLVRKP 437

Query: 425 MDNQQNTVTAQGRAEQKEITGLVIHNCRIVPEQKLFAERFKIPTFLGRPWKQYARTVIME 484
           + NQ   +TA GR + +E TG V   C I  E    A +     +LGRPWK+Y+RT+IM 
Sbjct: 438 LPNQACPITAHGRKDPRESTGFVFQGCTIAGEPDYLAVKETSKAYLGRPWKEYSRTIIMN 497

Query: 485 STLGDFIQPAGYMPWSGDFALETCLYLEYGNRGPGANTNRRVRWKGAKVIGRNEALQYTA 544
           + + DF+QP G+ PW GDF L+T  Y E  N GPG+    RV W G K +   + L++T 
Sbjct: 498 TFIPDFVQPQGWQPWLGDFGLKTLFYSEVQNTGPGSALANRVTWAGIKTLSEEDILKFTP 557

Query: 545 GAFLLGRSWLPTTGGLYYLGL 566
             ++ G  W+P  G  Y  GL
Sbjct: 558 AQYIQGDDWIPGKGVPYTTGL 565

[Close]

BLAST of CSPI05G02400 vs. TAIR 10
Match: AT2G47030.1 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 427.9 bits (1099), Expect = 1.2e-119
Identity = 255/604 (42.22%), Postives = 358/604 (59.27%), Query Frame = 0


Query: 1   MLGKVIVSGLSLILVVGIALAIAATINKSNASNGGTEDVSPKMKAVSAICSTTDYQDECK 60
           M+GKV+VS  S++L+VG+A+ + A INK+     G  ++SP+MKAV  IC +T  +  C 
Sbjct: 1   MIGKVVVSVASILLIVGVAIGVVAFINKN-----GDANLSPQMKAVQGICQSTSDKASCV 60

Query: 61  TTLDHVARNTSSNDPKDYAEAAILATIGEITKGYNLSDSLIVE--ASTNASIKMSVEDCK 120
            TL+ V     S DP    +A +LAT  E+TK  N +    V   +S + + K  ++ CK
Sbjct: 61  KTLEPV----KSEDPNKLIKAFMLATKDELTKSSNFTGQTEVNMGSSISPNNKAVLDYCK 120

Query: 121 DLLQFAIDELQASYSAVGESDLHTDADRVADIKNWLSAVISYQQSCLDGLGEFDPQLKQR 180
            +  +A+++L      +GE DL     ++  +K WL  V +YQ  CLD + E D  L++ 
Sbjct: 121 RVFMYALEDLATIIEEMGE-DLSQIGSKIDQLKQWLIGVYNYQTDCLDDIEEDD--LRKA 180

Query: 181 MQDGLDVAGKLTSNALAI-------VTAVSNILDNYRLQLKVQPS--------------- 240
           + +G+  +  LT+NA+ I       +  ++N +D+ +      P+               
Sbjct: 181 IGEGIANSKILTTNAIDIFHTVVSAMAKINNKVDDLKNMTGGIPTPGAPPVVDESPVADP 240

Query: 241 ---GRRLLGTTVVDRDGFPTWLTGADRKLLA--------SKQGGVRPTPNAVVAKDGSGK 300
               RRLL    +D  G PTW++GADRKL+A         + GG R   N VVAKDGSG+
Sbjct: 241 DGPARRLLED--IDETGIPTWVSGADRKLMAKAGRGRRGGRGGGARVRTNFVVAKDGSGQ 300

Query: 301 YKTIAAALAAYPKVLRGRYVIYVKAGIYDEYITLTKDMKNVFMYGDGPRKTIVTGRKS-- 360
           +KT+  A+ A P+  RGR +IY+KAG+Y E + + K   N+FM+GDG RKT+++  +S  
Sbjct: 301 FKTVQQAVDACPENNRGRCIIYIKAGLYREQVIIPKKKNNIFMFGDGARKTVISYNRSVA 360

Query: 361 NRDGFTTQNTASFAAIGEGFLCKSMGFTNTAGPEGHQAVALRVQSDRSAFFNCRMDGHQD 420
              G TT  +A+     EGF+ K MGF NTAGP GHQA A+RV  DR+  FNCR DG+QD
Sbjct: 361 LSRGTTTSLSATVQVESEGFMAKWMGFKNTAGPMGHQAAAIRVNGDRAVIFNCRFDGYQD 420

Query: 421 TLYVQTHRQFYRNCVVSGTVDFIFGDSSTVIQNSLIIVRRPMDNQQNTVTAQGRAEQKEI 480
           TLYV   RQFYRNCVVSGTVDFIFG S+TVIQN+LI+VR+    Q NTVTA G      +
Sbjct: 421 TLYVNNGRQFYRNCVVSGTVDFIFGKSATVIQNTLIVVRKGSKGQYNTVTADGNELGLGM 480

Query: 481 -TGLVIHNCRIVPEQKLFAERFKIPTFLGRPWKQYARTVIMESTLGDFIQPAGYMPWSGD 540
             G+V+ NCRIVP++KL  ER  + T+LGRPWK+++ TVIM + +GD I+P G+  W G+
Sbjct: 481 KIGIVLQNCRIVPDRKLTPERLTVATYLGRPWKKFSTTVIMSTEMGDLIRPEGWKIWDGE 540

Query: 541 FALETCLYLEYGNRGPGANTNRRVRWKGAKVI-GRNEALQYTAGAFLLGRSWL-----PT 561
              ++C Y+EY NRGPGA  NRRV W  AKV     E   +TA  +L   +W+     P 
Sbjct: 541 SFHKSCRYVEYNNRGPGAFANRRVNW--AKVARSAAEVNGFTAANWLGPINWIQEANVPV 588

[Close]

BLAST of CSPI05G02400 vs. TAIR 10
Match: AT4G33230.1 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 427.9 bits (1099), Expect = 1.2e-119
Identity = 248/600 (41.33%), Postives = 354/600 (59.00%), Query Frame = 0


Query: 4   KVIVSGLSLILVVGIALAIAA----------------TINKSNASNGGTEDVSPKM---- 63
           K I+ G+  +LVV  A+   A                T NKS  S   +E  SPK     
Sbjct: 23  KRIILGVVSVLVVAAAIIGGAFAYVTYENKTQEQGKTTNNKSKDSPTKSESPSPKPPSSA 82

Query: 64  ----------KAVSAICSTTDYQDECKTTL-DHVARNTSSNDPKDYAEAAILATIGEITK 123
                     K +  +C++T Y+  C+ TL +   ++T   DP+   ++AI+A   ++ +
Sbjct: 83  AQTVKAGQVDKIIQTLCNSTLYKPTCQNTLKNETKKDTPQTDPRSLLKSAIVAVNDDLDQ 142

Query: 124 GYNLSDSLIVEASTNASIKMSVEDCKDLLQFAIDELQASYSAVGESDLHTDADRVADIKN 183
            +    SL  E   N   K ++  CK L+  A +EL  S   + +S+++  A  V D+ +
Sbjct: 143 VFKRVLSLKTE---NKDDKDAIAQCKLLVDEAKEELGTSMKRINDSEVNNFAKIVPDLDS 202

Query: 184 WLSAVISYQQSCLDGLGEFDPQLKQRMQDGLDVAGKLTSNALAIVTAVSNILDNY----- 243
           WLSAV+SYQ++C+DG  E   +LK  ++   + +  LTSN+LA++ ++   L +      
Sbjct: 203 WLSAVMSYQETCVDGFEE--GKLKTEIRKNFNSSQVLTSNSLAMIKSLDGYLSSVPKVKT 262

Query: 244 RLQLKVQPSGRRLLGTTVVDRDGFPTWLTGADRKLLASKQGGVRP-TPNAVVAKDGSGKY 303
           RL L+ + S +        + D   +WL+  +R++L  K   V+   PNA VAKDGSG +
Sbjct: 263 RLLLEARSSAK--------ETDHITSWLSNKERRML--KAVDVKALKPNATVAKDGSGNF 322

Query: 304 KTIAAALAAYPKVLRGRYVIYVKAGIYDEYITLTKDMKNVFMYGDGPRKTIVTGRKSNRD 363
            TI AAL A P   +GRY IY+K GIYDE + + K   NV M GDG +KTIVTG KS+  
Sbjct: 323 TTINAALKAMPAKYQGRYTIYIKHGIYDESVIIDKKKPNVTMVGDGSQKTIVTGNKSHAK 382

Query: 364 GFTTQNTASFAAIGEGFLCKSMGFTNTAGPEGHQAVALRVQSDRSAFFNCRMDGHQDTLY 423
              T  TA+F A GEGF+ +SMGF NTAGPEGHQAVA+RVQSDRS F NCR +G+QDTLY
Sbjct: 383 KIRTFLTATFVAQGEGFMAQSMGFRNTAGPEGHQAVAIRVQSDRSVFLNCRFEGYQDTLY 442

Query: 424 VQTHRQFYRNCVVSGTVDFIFGDSSTVIQNSLIIVRRPMDNQQNTVTAQGRAEQKEITGL 483
             THRQ+YR+CV+ GTVDFIFGD++ + QN  I +R+ +  Q+NTVTAQGR ++ + TG 
Sbjct: 443 AYTHRQYYRSCVIIGTVDFIFGDAAAIFQNCDIFIRKGLPGQKNTVTAQGRVDKFQTTGF 502

Query: 484 VIHNCRIVPEQKLFAERFKIPTFLGRPWKQYARTVIMESTLGDFIQPAGYMPW-SGDFAL 543
           VIHNC + P + L   + +  ++LGRPWK ++RTV+MEST+ D I P G++ W   DFA+
Sbjct: 503 VIHNCTVAPNEDLKPVKAQFKSYLGRPWKPHSRTVVMESTIEDVIDPVGWLRWQETDFAI 562

Query: 544 ETCLYLEYGNRGPGANTNRRVRWKGAKVIGRNEALQYTAGAFLLGRSWLPTTGGLYYLGL 566
           +T  Y EY N GP   T  RV+W G +V+ + EA+++T G FL G  W+   G    LGL
Sbjct: 563 DTLSYAEYKNDGPSGATAARVKWPGFRVLNKEEAMKFTVGPFLQG-EWIQAIGSPVKLGL 606

[Close]

BLAST of CSPI05G02400 vs. TAIR 10
Match: AT2G26450.1 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 424.9 bits (1091), Expect = 1.0e-118
Identity = 228/524 (43.51%), Postives = 330/524 (62.98%), Query Frame = 0


Query: 44  KAVSAICSTTDYQDECKTTL-DHVARNTSSNDPKDYAEAAILATIGEITKGYNLSDSLIV 103
           K +  +CS+T Y   C+ TL +   +  + ++P  + ++AI A   ++     + + ++ 
Sbjct: 107 KIIQTLCSSTLYMQICEKTLKNRTDKGFALDNPTTFLKSAIEAVNEDLDL---VLEKVLS 166

Query: 104 EASTNASIKMSVEDCKDLLQFAIDELQASYSAVGESDLHTDADRVADIKNWLSAVISYQQ 163
             + N   K ++E CK L++ A +E  AS + +  +++++    V D+++WLSAV+SYQ+
Sbjct: 167 LKTENQDDKDAIEQCKLLVEDAKEETVASLNKINVTEVNSFEKVVPDLESWLSAVMSYQE 226

Query: 164 SCLDGLGEFDPQLKQRMQDGLDVAGKLTSNALAIVTAVSNILDNYRLQLKVQPSGRRLLG 223
           +CLDG  E    LK  ++  ++ +  LTSN+LA+   +    +N    +KV    R LL 
Sbjct: 227 TCLDGFEE--GNLKSEVKTSVNSSQVLTSNSLAL---IKTFTENLSPVMKVVE--RHLL- 286

Query: 224 TTVVDRDGFPTWLTGADRKLLASKQGGVRPTPNAVVAKDGSGKYKTIAAALAAYPKVLRG 283
                 D  P+W++  DR++L +        PNA VAKDGSG + TI  AL A P+   G
Sbjct: 287 ------DDIPSWVSNDDRRMLRAVDVKAL-KPNATVAKDGSGDFTTINDALRAMPEKYEG 346

Query: 284 RYVIYVKAGIYDEYITLTKDMKNVFMYGDGPRKTIVTGRKSNRDGFTTQNTASFAAIGEG 343
           RY+IYVK GIYDEY+T+ K   N+ M GDG +KTIVTG KS+     T  TA+F A GEG
Sbjct: 347 RYIIYVKQGIYDEYVTVDKKKANLTMVGDGSQKTIVTGNKSHAKKIRTFLTATFVAQGEG 406

Query: 344 FLCKSMGFTNTAGPEGHQAVALRVQSDRSAFFNCRMDGHQDTLYVQTHRQFYRNCVVSGT 403
           F+ +SMGF NTAGPEGHQAVA+RVQSDRS F NCR +G+QDTLY  THRQ+YR+CV+ GT
Sbjct: 407 FMAQSMGFRNTAGPEGHQAVAIRVQSDRSIFLNCRFEGYQDTLYAYTHRQYYRSCVIVGT 466

Query: 404 VDFIFGDSSTVIQNSLIIVRRPMDNQQNTVTAQGRAEQKEITGLVIHNCRIVPEQKLFAE 463
           +DFIFGD++ + QN  I +R+ +  Q+NTVTAQGR ++ + TG V+HNC+I   + L   
Sbjct: 467 IDFIFGDAAAIFQNCNIFIRKGLPGQKNTVTAQGRVDKFQTTGFVVHNCKIAANEDLKPV 526

Query: 464 RFKIPTFLGRPWKQYARTVIMESTLGDFIQPAGYMPW-SGDFALETCLYLEYGNRGPGAN 523
           + +  ++LGRPWK Y+RT+IMES + + I P G++ W   DFA++T  Y EY N+G   +
Sbjct: 527 KEEYKSYLGRPWKNYSRTIIMESKIENVIDPVGWLRWQETDFAIDTLYYAEYNNKGSSGD 586

Query: 524 TNRRVRWKGAKVIGRNEALQYTAGAFLLGRSWLPTTGGLYYLGL 566
           T  RV+W G KVI + EAL YT G FL G  W+  +G    LGL
Sbjct: 587 TTSRVKWPGFKVINKEEALNYTVGPFLQG-DWISASGSPVKLGL 611

The following BLAST results are available for this feature:

BLAST of CSPI05G02400 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q3E8Z8	1.4e-123	39.32	Putative pectinesterase/pectinesterase inhibitor 28 OS=Arabidopsis thaliana OX=3...	[more]
Q8GX86	6.8e-123	39.75	Probable pectinesterase/pectinesterase inhibitor 21 OS=Arabidopsis thaliana OX=3...	[more]
Q9SMY6	1.7e-118	41.33	Putative pectinesterase/pectinesterase inhibitor 45 OS=Arabidopsis thaliana OX=3...	[more]
O80722	1.7e-118	42.22	Pectinesterase 4 OS=Arabidopsis thaliana OX=3702 GN=PME4 PE=2 SV=1	[more]
Q7Y201	1.5e-117	43.51	Probable pectinesterase/pectinesterase inhibitor 13 OS=Arabidopsis thaliana OX=3...	[more]

BLAST of CSPI05G02400 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0KJ73	0.0e+00	99.65	Pectinesterase OS=Cucumis sativus OX=3659 GN=Csa_5G125700 PE=3 SV=1	[more]
A0A5A7U4V7	0.0e+00	94.00	Pectinesterase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold180G00110...	[more]
A0A1S3CSH2	6.3e-310	93.65	Pectinesterase OS=Cucumis melo OX=3656 GN=LOC103503817 PE=3 SV=1	[more]
A0A0A0KLN2	1.1e-269	81.31	Pectinesterase OS=Cucumis sativus OX=3659 GN=Csa_5G119700 PE=3 SV=1	[more]
A0A5A7U7W4	1.0e-267	81.66	Pectinesterase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold180G00090...	[more]

BLAST of CSPI05G02400 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_004149499.1	0.0e+00	99.65	pectinesterase [Cucumis sativus] >KGN49780.1 hypothetical protein Csa_004678 [Cu...	[more]
KAA0050274.1	0.0e+00	94.00	pectinesterase-like [Cucumis melo var. makuwa] >TYJ98133.1 pectinesterase-like [...	[more]
XP_008466403.1	1.3e-309	93.65	PREDICTED: pectinesterase-like [Cucumis melo]	[more]
XP_038875889.1	1.2e-281	85.21	pectinesterase-like [Benincasa hispida]	[more]
XP_038875879.1	1.0e-272	82.72	LOW QUALITY PROTEIN: pectinesterase-like [Benincasa hispida]	[more]

BLAST of CSPI05G02400 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G27870.1	9.8e-125	39.32	Plant invertase/pectin methylesterase inhibitor superfamily	[more]
AT3G05610.1	4.8e-124	39.75	Plant invertase/pectin methylesterase inhibitor superfamily	[more]
AT2G47030.1	1.2e-119	42.22	Plant invertase/pectin methylesterase inhibitor superfamily	[more]
AT4G33230.1	1.2e-119	41.33	Plant invertase/pectin methylesterase inhibitor superfamily	[more]
AT2G26450.1	1.0e-118	43.51	Plant invertase/pectin methylesterase inhibitor superfamily	[more]

InterPro

Analysis Name: InterPro Annotations of Cucumber (PI 183967) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR006501	Pectinesterase inhibitor domain	SMART	SM00856	PMEI_2	coord: 41..196 e-value: 1.7E-34 score: 130.6
IPR006501	Pectinesterase inhibitor domain	TIGRFAM	TIGR01614	TIGR01614	coord: 34..198 e-value: 3.6E-18 score: 63.5
IPR006501	Pectinesterase inhibitor domain	PFAM	PF04043	PMEI	coord: 45..196 e-value: 4.4E-25 score: 88.8
IPR035513	Invertase/pectin methylesterase inhibitor domain superfamily	GENE3D	1.20.140.40	Invertase/pectin methylesterase inhibitor family protein	coord: 43..201 e-value: 2.7E-31 score: 110.6
IPR035513	Invertase/pectin methylesterase inhibitor domain superfamily	SUPERFAMILY	101148	Plant invertase/pectin methylesterase inhibitor	coord: 44..200
IPR000070	Pectinesterase, catalytic	PFAM	PF01095	Pectinesterase	coord: 255..550 e-value: 2.4E-129 score: 430.3
IPR012334	Pectin lyase fold	GENE3D	2.160.20.10		coord: 250..565 e-value: 1.2E-106 score: 358.7
None	No IPR available	PANTHER	PTHR31707	PECTINESTERASE	coord: 1..565
None	No IPR available	PANTHER	PTHR31707:SF292	PECTINESTERASE	coord: 1..565
None	No IPR available	CDD	cd15798	PMEI-like_3	coord: 48..202 e-value: 8.21784E-50 score: 167.23
IPR033131	Pectinesterase, Asp active site	PROSITE	PS00503	PECTINESTERASE_2	coord: 399..408
IPR011050	Pectin lyase fold/virulence factor	SUPERFAMILY	51126	Pectin lyase-like	coord: 252..561

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CSPI05G02400.1	CSPI05G02400.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0042545	cell wall modification
biological_process	GO:0043086	negative regulation of catalytic activity
biological_process	GO:0045490	pectin catabolic process
cellular_component	GO:0005576	extracellular region
molecular_function	GO:0045330	aspartyl esterase activity
molecular_function	GO:0030599	pectinesterase activity
molecular_function	GO:0046910	pectinesterase inhibitor activity
molecular_function	GO:0004857	enzyme inhibitor activity