CSPI04G23190 (gene) Cucumber (PI 183967) v1

Overview
NameCSPI04G23190
Typegene
OrganismCucumis sativus var. hardwickii cv. PI 183967 (Cucumber (PI 183967) v1)
DescriptionD-amino-acid transaminase, chloroplastic-like
LocationChr4: 21322621 .. 21325355 (-)
RNA-Seq ExpressionCSPI04G23190
SyntenyCSPI04G23190
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
CAATAATGTACTATCAACTCTAAATCAACGTGGATTCTAATCCGAATATTGTCGATATAAGAAATAGAAAATGGCAACTGCCGGCCTATTGGCGTGTATGAGATTTTTTAGTGAATGTTTGTTCATTCTCTGCTGCTGTATTCTTATTCTTACACCTTTTGCCACGAATCTTTTGGCCAAAGAATGCGCCGATTTACACTGCCACACAGCCATACTCTTCCAAAATCACAATCAGATTAAAGGGGAATTTGATCTCCCTCTACCCTCCAAATACGTATCGACAAACTTTCAGATAGTCAATTCCAAAAGATTCTTTCTTTTCACAAATGTTCACTCGAATACGACTTAAACCCAAAAGATTACAGTCGAAGTGGTTAGTTTGGATGTTTTTTTATATCGATCTTTACGCCCTTTTATGTTGGCCACTTTTCATGCTTCTGCACTCCAAGTTCTGTAAATTTTGTTTGAGTTCTGTTCAATGGAGGCTTTGAATTCTTCTTCTAGTGAAATTCAAGGTGGGATTTTCTTTTTTTCTGTTTTTTTCTTTTCATGTGGGGTTTGAGATTTTCGTGGGTTTTTTTTGGTCTGGTCTTTTGTTGTTGTTAATATGATTGTTTCTGTTTGTGTGATGATGCATTAGCGATTGGAAATGGAAGTGAAACTGACTTGAAGGTTCCTGTGTTCTCATCATCTTTGGAGGTAAATTCTTGAAACCCTATATTTGGTTGATCTTTTTTGCCCCTTACATGTTTGTATTGTATGTTTTTCATTTGGCATGTAGAAAGTTTGAGATAGTTAGTCGAGGCTCTGAGGTACTGAATCAATCTACTAATAGAAAGGATTGGATTATGGTTTCGTTAAATTTGTTCTCTATATGGTTGAACTTCCATGTCTCGAGTCTCTGGACCTGTTAGAACCTGTTTGAGTAAAAAAAAAATTGTGATAGAATCCAAATAAATTGAAATATAAATGATATCAGAACTAAGAGATATCTCTAAAGTCTTAATTCACTAACCAAAATAATGGTTTCGTTTCTCATTCTCCTTCTCCCTAAACCCTAAACCCTACTTCTCCCTACTTATAGCCAAATAGCACAAGTAGAGAATTAATTGGTTGTTGAAGGTGAGAGACATTGTTGTATGAGATTATGCAGGAGACATTTTTCTTGAGAAGGAAAGAGTAATCTGATATAGTTGAGTTTTCTACTGGCTTTTAGAAAGTATCATCATTGTTGCAATGCAATTATTTTGCTCTTCCTTTCAAACAGTTGCTCGAAAAACTTCACGAGAAATGGAGTTTGGTCAACAAGAAACCGTATCCAGCAATGTATTCCAGTGTTTTTGGTGGGATTATTACAGATCCAGCTATGATGGTGATTCCTATCGATGACCACATGGTTCATCGAGGCCACGGCGTGTTTGATACAGCCATTATTTTAAATGGGTTGGTACATGGGAATATTTCATCAAATTTATATCAGTTCCTCCTAATTTCTCAAGCTGCTATAACTGTTACTTCCTTACTGGCTTTCCTTGTTTTAAAACCTTTGTTTTGGTCTTTAACAGATATTTGTATGAGTTGGATGCCCACATCGACCGCTTTCTAAGATCGGCTTCAAAAGCAAAGATCTCTCCTCCCTTTCCACGGTCAATTCTTCGGAGCATTCTTATCCAATTGACAGCAGTATCACAGCTCAAGAAAGGCACTTTAAGATATTGGTTAAGTGCAGGACCTGGAGATTTCTTGCTCACGCCTGCCGTTGATGCAAATTCTGCATTTTATGCAGCAGCCATTGACGACGACTTCTCTCAATGCAAAGAAGGGGTTAAAGCGATAACATCCACAATCCCGATGAAGACACCTCAGTTTGCAACAATGAAGAATGTGAACTACCTACCAAATGTACTAGCCAAACTGGAAGCTGAAGAGAAAGGGGCATTTGCTTCCATTTGGGTTGATGAAGAAGGCTATATTGCAGAAGGTCCAAATGTGAACGTTGCATTTATAACCAATGAAAAGGAGCTTATCCTGCCTTCTTTCGACAAGATTCTCTCAGGTTGCACTGCACTGCGACTTCTTAAGTTAGCTCCGAAGTTGGTCAAGGAAGGGAAGCTGAAGAGTGTTGGAACTGCAAACCTTACGGTTAAGGAAGCCAAAGACGCTGCCGAAATGATGTTTGTAGGAAGCACACTGCCGCTGCTGCCAATCATCTCATGGGATGAAGAACCTATTGGAGATGGTAATATCTATGGACTGATCTAAACTTCTGTTAAAGTGATAATGAATGAAATGTTCTTATAAGCAACTCTATTGATTCTCTGTTACAGGACGCGTAGGAGAACTGACGATGGCACTTTCAGATCTGCTTTGGGATGATATGGTCTCAGGCCCTGAAACAGAGAGAATACCAGTTTCATACACAGAGTGAAACCAAGCCCCAACAGAATCAAAGGATGTCAAAGGCTTGCTTTTCCTATGGTCATATCTGCTTTCTTTGAACGTGAATAAAAGGAAAATATCCTATTGGACATGTTCCTAATAGCGATCGTTTTTCGGTTATGTTGTGTATATATTCATTGCGAATTCAGTTGTTTTACCTTTGGAAACTCGATGCTTGCATCAGTTGAAAAGACAGACCGGTGTGCATTTATCCAAGCTGAGCTATGCTTCAAGTTTATTGAAGCTGACGATGAATATTAAAATGAAGGTCTACTGGAAGATAAATTTTGCAGCAC

mRNA sequence

CAATAATGTACTATCAACTCTAAATCAACGTGGATTCTAATCCGAATATTGTCGATATAAGAAATAGAAAATGGCAACTGCCGGCCTATTGGCGTGTATGAGATTTTTTAGTGAATGTTTGTTCATTCTCTGCTGCTGTATTCTTATTCTTACACCTTTTGCCACGAATCTTTTGGCCAAAGAATGCGCCGATTTACACTGCCACACAGCCATACTCTTCCAAAATCACAATCAGATTAAAGGGGAATTTGATCTCCCTCTACCCTCCAAATACGTATCGACAAACTTTCAGATAGTCAATTCCAAAAGATTCTTTCTTTTCACAAATGTTCACTCGAATACGACTTAAACCCAAAAGATTACAGTCGAAGTGGTTAGTTTGGATGTTTTTTTATATCGATCTTTACGCCCTTTTATGTTGGCCACTTTTCATGCTTCTGCACTCCAAGTTCTGTAAATTTTGTTTGAGTTCTGTTCAATGGAGGCTTTGAATTCTTCTTCTAGTGAAATTCAAGCGATTGGAAATGGAAGTGAAACTGACTTGAAGGTTCCTGTGTTCTCATCATCTTTGGAGTTGCTCGAAAAACTTCACGAGAAATGGAGTTTGGTCAACAAGAAACCGTATCCAGCAATGTATTCCAGTGTTTTTGGTGGGATTATTACAGATCCAGCTATGATGGTGATTCCTATCGATGACCACATGGTTCATCGAGGCCACGGCGTGTTTGATACAGCCATTATTTTAAATGGATATTTGTATGAGTTGGATGCCCACATCGACCGCTTTCTAAGATCGGCTTCAAAAGCAAAGATCTCTCCTCCCTTTCCACGGTCAATTCTTCGGAGCATTCTTATCCAATTGACAGCAGTATCACAGCTCAAGAAAGGCACTTTAAGATATTGGTTAAGTGCAGGACCTGGAGATTTCTTGCTCACGCCTGCCGTTGATGCAAATTCTGCATTTTATGCAGCAGCCATTGACGACGACTTCTCTCAATGCAAAGAAGGGGTTAAAGCGATAACATCCACAATCCCGATGAAGACACCTCAGTTTGCAACAATGAAGAATGTGAACTACCTACCAAATGTACTAGCCAAACTGGAAGCTGAAGAGAAAGGGGCATTTGCTTCCATTTGGGTTGATGAAGAAGGCTATATTGCAGAAGGTCCAAATGTGAACGTTGCATTTATAACCAATGAAAAGGAGCTTATCCTGCCTTCTTTCGACAAGATTCTCTCAGGTTGCACTGCACTGCGACTTCTTAAGTTAGCTCCGAAGTTGGTCAAGGAAGGGAAGCTGAAGAGTGTTGGAACTGCAAACCTTACGGTTAAGGAAGCCAAAGACGCTGCCGAAATGATGTTTGTAGGAAGCACACTGCCGCTGCTGCCAATCATCTCATGGGATGAAGAACCTATTGGAGATGGACGCGTAGGAGAACTGACGATGGCACTTTCAGATCTGCTTTGGGATGATATGGTCTCAGGCCCTGAAACAGAGAGAATACCAGTTTCATACACAGAGTGAAACCAAGCCCCAACAGAATCAAAGGATGTCAAAGGCTTGCTTTTCCTATGGTCATATCTGCTTTCTTTGAACGTGAATAAAAGGAAAATATCCTATTGGACATGTTCCTAATAGCGATCGTTTTTCGGTTATGTTGTGTATATATTCATTGCGAATTCAGTTGTTTTACCTTTGGAAACTCGATGCTTGCATCAGTTGAAAAGACAGACCGGTGTGCATTTATCCAAGCTGAGCTATGCTTCAAGTTTATTGAAGCTGACGATGAATATTAAAATGAAGGTCTACTGGAAGATAAATTTTGCAGCAC

Coding sequence (CDS)

ATGGAGGCTTTGAATTCTTCTTCTAGTGAAATTCAAGCGATTGGAAATGGAAGTGAAACTGACTTGAAGGTTCCTGTGTTCTCATCATCTTTGGAGTTGCTCGAAAAACTTCACGAGAAATGGAGTTTGGTCAACAAGAAACCGTATCCAGCAATGTATTCCAGTGTTTTTGGTGGGATTATTACAGATCCAGCTATGATGGTGATTCCTATCGATGACCACATGGTTCATCGAGGCCACGGCGTGTTTGATACAGCCATTATTTTAAATGGATATTTGTATGAGTTGGATGCCCACATCGACCGCTTTCTAAGATCGGCTTCAAAAGCAAAGATCTCTCCTCCCTTTCCACGGTCAATTCTTCGGAGCATTCTTATCCAATTGACAGCAGTATCACAGCTCAAGAAAGGCACTTTAAGATATTGGTTAAGTGCAGGACCTGGAGATTTCTTGCTCACGCCTGCCGTTGATGCAAATTCTGCATTTTATGCAGCAGCCATTGACGACGACTTCTCTCAATGCAAAGAAGGGGTTAAAGCGATAACATCCACAATCCCGATGAAGACACCTCAGTTTGCAACAATGAAGAATGTGAACTACCTACCAAATGTACTAGCCAAACTGGAAGCTGAAGAGAAAGGGGCATTTGCTTCCATTTGGGTTGATGAAGAAGGCTATATTGCAGAAGGTCCAAATGTGAACGTTGCATTTATAACCAATGAAAAGGAGCTTATCCTGCCTTCTTTCGACAAGATTCTCTCAGGTTGCACTGCACTGCGACTTCTTAAGTTAGCTCCGAAGTTGGTCAAGGAAGGGAAGCTGAAGAGTGTTGGAACTGCAAACCTTACGGTTAAGGAAGCCAAAGACGCTGCCGAAATGATGTTTGTAGGAAGCACACTGCCGCTGCTGCCAATCATCTCATGGGATGAAGAACCTATTGGAGATGGACGCGTAGGAGAACTGACGATGGCACTTTCAGATCTGCTTTGGGATGATATGGTCTCAGGCCCTGAAACAGAGAGAATACCAGTTTCATACACAGAGTGA

Protein sequence

MEALNSSSSEIQAIGNGSETDLKVPVFSSSLELLEKLHEKWSLVNKKPYPAMYSSVFGGIITDPAMMVIPIDDHMVHRGHGVFDTAIILNGYLYELDAHIDRFLRSASKAKISPPFPRSILRSILIQLTAVSQLKKGTLRYWLSAGPGDFLLTPAVDANSAFYAAAIDDDFSQCKEGVKAITSTIPMKTPQFATMKNVNYLPNVLAKLEAEEKGAFASIWVDEEGYIAEGPNVNVAFITNEKELILPSFDKILSGCTALRLLKLAPKLVKEGKLKSVGTANLTVKEAKDAAEMMFVGSTLPLLPIISWDEEPIGDGRVGELTMALSDLLWDDMVSGPETERIPVSYTE*
Homology
[Close]
BLAST of CSPI04G23190 vs. ExPASy Swiss-Prot
Match: Q8L493 (D-amino-acid transaminase, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=DAAT PE=1 SV=1)

HSP 1 Score: 362.1 bits (928), Expect = 7.2e-99
Identity = 186/332 (56.02%), Postives = 240/332 (72.29%), Query Frame = 0 

Query: 16  NGSETDLKVPVFSSSLELLEKLHEKWSLVNKKPYPAMYSSVFGGIITDPAMMVIPIDDHM 75
           + S     VPV  SS E+ E+L         + + AMYSSV  GI TDPA MV+P+DDHM
Sbjct: 46  DSSSQSWNVPVL-SSYEVGERLK---LARGGQQFLAMYSSVVDGITTDPAAMVLPLDDHM 105

Query: 76  VHRGHGVFDTAIILNGYLYELDAHIDRFLRSASKAKISPPFPRSILRSILIQLTAVSQLK 135
           VHRGHGVFDTA+I+NGYLYELD H+DR LRSAS AKI  PF R  ++ ILIQ  +VS  +
Sbjct: 106 VHRGHGVFDTALIINGYLYELDQHLDRILRSASMAKIPLPFDRETIKRILIQTVSVSGCR 165

Query: 136 KGTLRYWLSAGPGDFLLTPAVDANSAFYAAAIDDDFSQCKEGVKAITSTIPMKTPQFATM 195
            G+LRYWLSAGPGDFLL+P+       YA  I  +F+    GVK +TS+IP+K P+FAT+
Sbjct: 166 DGSLRYWLSAGPGDFLLSPSQCLKPTLYAIVIKTNFAINPIGVKVVTSSIPIKPPEFATV 225

Query: 196 KNVNYLPNVLAKLEAEEKGAFASIWVDEEGYIAEGPNVNVAFITN-EKELILPSFDKILS 255
           K+VNYLPNVL+++EAE KGA+A IWV ++G+IAEGPN+NVAF+ N  KEL++P FD +LS
Sbjct: 226 KSVNYLPNVLSQMEAEAKGAYAGIWVCKDGFIAEGPNMNVAFVVNGGKELVMPRFDNVLS 285

Query: 256 GCTALRLLKLAPKLVKEGKLKSVGTANLTVKEAKDAAEMMFVGSTLPLLPIISWDEEPIG 315
           GCTA R L LA +LV +G LK+V   ++TV++ K A EMM +GS +P+ P+I WDEE IG
Sbjct: 286 GCTAKRTLTLAEQLVSKGILKTVKVMDVTVEDGKKADEMMLIGSGIPIRPVIQWDEEFIG 345

Query: 316 DGRVGELTMALSDLLWDDMVSGPETERIPVSY 347
           +G+ G +  AL DLL +DM SGP + R+ V Y
Sbjct: 346 EGKEGPIAKALLDLLLEDMRSGPPSVRVLVPY 373

[Close]
BLAST of CSPI04G23190 vs. ExPASy Swiss-Prot
Match: Q58414 (Putative branched-chain-amino-acid aminotransferase OS=Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440) OX=243232 GN=ilvE PE=3 SV=1)

HSP 1 Score: 104.0 bits (258), Expect = 3.5e-21
Identity = 75/276 (27.17%), Postives = 131/276 (47.46%), Query Frame = 0 

Query: 59  GIITDPAMMVIPIDDHMVHRGHGVFDTAIILNGYLYELDAHIDRFLRSASKAKISPPFPR 118
           G   D     + + DH +  G GVF+     +G ++ L  HIDR   SA    I  P  +
Sbjct: 7   GKFVDEKDAKVSVFDHGLLYGDGVFEGIRAYDGVVFMLKEHIDRLYDSAKSLCIDIPLTK 66

Query: 119 SILRSILIQLTAVSQLKKGTLRYWLSAGPGDFLLTPAVDANSAFYAAAIDDDFSQCKEGV 178
             +  ++++   V+ L+   +R  ++ G GD  L P        +  AI       ++G+
Sbjct: 67  EEMIDVVLETLRVNNLRDAYIRLVVTRGVGDLGLDPRKCGKPTIFCIAIPMPPLLGEDGI 126

Query: 179 KAITSTI---PMKTPQFATMKNVNYLPNVLAKLEAEEKGAFASIWVDEEGYIAEGPNVNV 238
           +AIT ++   P+     A +K++NYL +VLAK++A   G   +  +D++G++ EG   N+
Sbjct: 127 RAITVSVRRLPVDVLNPA-VKSLNYLNSVLAKIQANYAGVDEAFLLDDKGFVVEGTGDNI 186

Query: 239 AFITNEKELILPSFDKILSGCTALRLLKLAPKLVKEGKLKSVGTANLTVKEAKDAAEMMF 298
             + N      P +  IL G T   ++KLA    KE  ++ V    LT+ +   A E+  
Sbjct: 187 FIVKNGVLKTPPVYQSILKGITRDVVIKLA----KEEGIEVV-EEPLTLHDLYTADELFI 246

Query: 299 VGSTLPLLPIISWDEEPIGDGRVGELTMALSDLLWD 332
            G+   ++P+   D   I + +VGE+T  L +   D
Sbjct: 247 TGTAAEIVPVFEIDGRVINNKQVGEITKKLKEKFKD 276

[Close]
BLAST of CSPI04G23190 vs. ExPASy Swiss-Prot
Match: O29329 (Putative branched-chain-amino-acid aminotransferase OS=Archaeoglobus fulgidus (strain ATCC 49558 / VC-16 / DSM 4304 / JCM 9628 / NBRC 100126) OX=224325 GN=ilvE PE=1 SV=1)

HSP 1 Score: 99.8 bits (247), Expect = 6.7e-20
Identity = 76/284 (26.76%), Postives = 130/284 (45.77%), Query Frame = 0 

Query: 69  IPIDDHMVHRGHGVFDTAIILNGYLYELDAHIDRFLRSASKAKISPPFPRSILRSILIQL 128
           + I DH    G GVF+     NG ++ L  HIDR   SA    +  P  +     I+++ 
Sbjct: 18  VSIFDHGFLYGDGVFEGIRAYNGRVFRLKEHIDRLYDSAKAIDLEIPITKEEFMEIILET 77

Query: 129 TAVSQLKKGTLRYWLSAGPGDFLLTPAVDANSAF------YAAAIDDDFSQCKEGVKAIT 188
              + L+   +R  ++ G GD  L P    N +       +     D +   ++G+ AIT
Sbjct: 78  LRKNNLRDAYIRPIVTRGIGDLGLDPRKCQNPSIIVITKPWGKLYGDLY---EKGLTAIT 137

Query: 189 STIPMKTPQF--ATMKNVNYLPNVLAKLEAEEKGAFASIWVDEEGYIAEGPNVNVAFITN 248
             +   +       +K++NYL N+LAK+EA  KG   +I++D  GY++EG   N+ F+  
Sbjct: 138 VAVRRNSFDALPPNIKSLNYLNNILAKIEANAKGGDEAIFLDRNGYVSEGSGDNI-FVVK 197

Query: 249 EKELILPSFDKILSGCTALRLLKLAPKLVKEGKLKSVGTANLTVKEAKDAAEMMFVGSTL 308
              +  P     L G T   ++++  +L    K  ++G  +L       A E+   G+  
Sbjct: 198 NGAITTPPTINNLRGITREAVIEIINRLGIPFKETNIGLYDLYT-----ADEVFVTGTAA 257

Query: 309 PLLPIISWDEEPIGDGRVGELTMALSDLLWDDMVSGPETERIPV 345
            + PI+  D   IGDG+ GE+T      L ++     E+E +P+
Sbjct: 258 EIAPIVVIDGRKIGDGKPGEITRK----LMEEFSKLTESEGVPI 288

[Close]
BLAST of CSPI04G23190 vs. ExPASy Swiss-Prot
Match: O07597 (D-alanine aminotransferase OS=Bacillus subtilis (strain 168) OX=224308 GN=dat PE=3 SV=1)

HSP 1 Score: 86.7 bits (213), Expect = 5.8e-16
Identity = 68/259 (26.25%), Postives = 124/259 (47.88%), Query Frame = 0 

Query: 69  IPIDDHMVHRGHGVFDTAIILNGYLYELDAHIDRFLRSASKAKISPPFPRSILRSILIQL 128
           I ++D     G G+++   +  G L+ L  H +RF RSA++  IS PF    L   L +L
Sbjct: 17  IDLEDRGYQFGDGIYEVIRVYKGVLFGLREHAERFFRSAAEIGISLPFSIEDLEWDLQKL 76

Query: 129 TAVSQLKKGTLRYWLSAG--PGDFLLTPAVDANSAFYAAAIDDDFSQCKEGVKAITSTIP 188
              + + +G +    + G  P        ++  +  Y   +     +   GV AIT    
Sbjct: 77  VQENAVSEGAVYIQTTRGVAPRKHQYEAGLEPQTTAYTFTVKKPEQEQAYGVAAITDE-D 136

Query: 189 MKTPQFATMKNVNYLPNVLAKLEAEEKGAFASIWVDEEGYIAEGPNVNVAFITNEKELIL 248
           ++  +   +K++N L NV+ K  A E GAF +I +  +G + EG + NV  + N      
Sbjct: 137 LRWLR-CDIKSLNLLYNVMTKQRAYEAGAFEAILL-RDGVVTEGTSSNVYAVINGTVRTH 196

Query: 249 PSFDKILSGCTALRLLKLAPKLVKEGKLKSVGTANLTVKEAKDAAEMMFVGSTLPLLPII 308
           P+   IL+G T + +L     L+++  +K +    ++ +E K A E+    +T  ++P++
Sbjct: 197 PANRLILNGITRMNIL----GLIEKNGIK-LDETPVSEEELKQAEEIFISSTTAEIIPVV 256

Query: 309 SWDEEPIGDGRVGELTMAL 326
           + D + IG G+ G +T  L
Sbjct: 257 TLDGQSIGSGKPGPVTKQL 267

[Close]
BLAST of CSPI04G23190 vs. ExPASy Swiss-Prot
Match: P63511 (D-alanine aminotransferase OS=Staphylococcus aureus (strain Mu50 / ATCC 700699) OX=158878 GN=dat PE=3 SV=1)

HSP 1 Score: 78.6 bits (192), Expect = 1.6e-13
Identity = 69/272 (25.37%), Postives = 123/272 (45.22%), Query Frame = 0 

Query: 59  GIITDPAMMVIPIDDHMVHRGHGVFDTAIILNGYLYELDAHIDRFLRSASKAKISPPFPR 118
           G    P+   +  +D     G G+++   + NG L+ +  H +RFLRSA++  +   +  
Sbjct: 8   GEFVSPSEAKVSYNDRGYVFGDGIYEYIRVYNGKLFTVTEHYERFLRSANEIGLDLNYSV 67

Query: 119 SILRSILIQLTAVSQLKKGTLRYWLSAGPGD---FLLTPAVDANSAFYAAAIDDDFSQCK 178
             L  +  +L  ++Q++ G +    + G  +      TP V+     Y  + D  +   +
Sbjct: 68  EELIELSRKLVDMNQIETGAIYIQATRGVAERNHSFPTPEVEPAIVAYTKSYDRPYDHLE 127

Query: 179 EGVKAITSTIPMKTPQFATMKNVNYLPNVLAKLEAEEKGAFASIWVDEEGYIAEGPNVNV 238
            GV  +  T+         +K++N L NVLAK  A +  A  +I    E  + EG + N 
Sbjct: 128 NGVNGV--TVEDIRWLRCDIKSLNLLGNVLAKEYAVKYNAVEAIQHRGE-TVTEGSSSNA 187

Query: 239 AFITNEKELILPSFDKILSGCTALRLLKLAPKLVKEGKLKSVGTANLTVKEAKDAAEMMF 298
             I +      P  + IL+G T + + K+A       K ++      TV   K+A E++ 
Sbjct: 188 YAIKDGVIYTHPINNYILNGITRIVIKKIAEDYNIPFKEET-----FTVDFLKNADEVIV 247

Query: 299 VGSTLPLLPIISWDEEPIGDGRVGELTMALSD 328
             ++  + P+I  D EPI DG+VG +T  L +
Sbjct: 248 SSTSAEVTPVIKLDGEPINDGKVGPITRQLQE 271

[Close]
BLAST of CSPI04G23190 vs. ExPASy TrEMBL
Match: A0A0A0KZK6 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_4G629460 PE=4 SV=1)

HSP 1 Score: 686.4 bits (1770), Expect = 6.2e-194
Identity = 346/348 (99.43%), Postives = 347/348 (99.71%), Query Frame = 0 

Query: 1   MEALNSSSSEIQAIGNGSETDLKVPVFSSSLELLEKLHEKWSLVNKKPYPAMYSSVFGGI 60
           MEALNSSSSEIQAIGNGSETDLKVPVFSSSLELLEKLHEKWSLVNKKPYPAMYSSVFGGI
Sbjct: 1   MEALNSSSSEIQAIGNGSETDLKVPVFSSSLELLEKLHEKWSLVNKKPYPAMYSSVFGGI 60

Query: 61  ITDPAMMVIPIDDHMVHRGHGVFDTAIILNGYLYELDAHIDRFLRSASKAKISPPFPRSI 120
           ITDPAMMVIPIDDHMVHRGHGVFDTAIILNGYLYELDAHIDRFLRSASKAKISPPFPRSI
Sbjct: 61  ITDPAMMVIPIDDHMVHRGHGVFDTAIILNGYLYELDAHIDRFLRSASKAKISPPFPRSI 120

Query: 121 LRSILIQLTAVSQLKKGTLRYWLSAGPGDFLLTPAVDANSAFYAAAIDDDFSQCKEGVKA 180
           LRSILIQLTAVSQLKKGTLRYWLSAGPGDFLLTPAV+ANSAFYA AIDDDFSQCKEGVKA
Sbjct: 121 LRSILIQLTAVSQLKKGTLRYWLSAGPGDFLLTPAVNANSAFYAVAIDDDFSQCKEGVKA 180

Query: 181 ITSTIPMKTPQFATMKNVNYLPNVLAKLEAEEKGAFASIWVDEEGYIAEGPNVNVAFITN 240
           ITSTIPMKTPQFATMKNVNYLPNVLAKLEAEEKGAFASIWVDEEGYIAEGPNVNVAFITN
Sbjct: 181 ITSTIPMKTPQFATMKNVNYLPNVLAKLEAEEKGAFASIWVDEEGYIAEGPNVNVAFITN 240

Query: 241 EKELILPSFDKILSGCTALRLLKLAPKLVKEGKLKSVGTANLTVKEAKDAAEMMFVGSTL 300
           EKELILPSFDKILSGCTALRLLKLAPKLVKEGKLKSVGTANLTVKEAKDAAEMMFVGSTL
Sbjct: 241 EKELILPSFDKILSGCTALRLLKLAPKLVKEGKLKSVGTANLTVKEAKDAAEMMFVGSTL 300

Query: 301 PLLPIISWDEEPIGDGRVGELTMALSDLLWDDMVSGPETERIPVSYTE 349
           PLLPIISWDEEPIGDGRVGELTMALSDLLWDDMVSGPETERIPVSYTE
Sbjct: 301 PLLPIISWDEEPIGDGRVGELTMALSDLLWDDMVSGPETERIPVSYTE 348

[Close]
BLAST of CSPI04G23190 vs. ExPASy TrEMBL
Match: A0A1S3CJV5 (D-amino-acid transaminase, chloroplastic-like OS=Cucumis melo OX=3656 GN=LOC103501786 PE=4 SV=1)

HSP 1 Score: 669.8 bits (1727), Expect = 6.0e-189
Identity = 336/348 (96.55%), Postives = 340/348 (97.70%), Query Frame = 0 

Query: 1   MEALNSSSSEIQAIGNGSETDLKVPVFSSSLELLEKLHEKWSLVNKKPYPAMYSSVFGGI 60
           MEALNSSSSEIQAIGNGSETDLKVPVFSSSLELLEKLHEKWSLV KKPYPAMYSS+ GGI
Sbjct: 1   MEALNSSSSEIQAIGNGSETDLKVPVFSSSLELLEKLHEKWSLVEKKPYPAMYSSILGGI 60

Query: 61  ITDPAMMVIPIDDHMVHRGHGVFDTAIILNGYLYELDAHIDRFLRSASKAKISPPFPRSI 120
           ITDPAMMVIPIDDHMVHRGHGVFDTAIILNGYLYELDAHIDRFLRSASKAKISPPFPRSI
Sbjct: 61  ITDPAMMVIPIDDHMVHRGHGVFDTAIILNGYLYELDAHIDRFLRSASKAKISPPFPRSI 120

Query: 121 LRSILIQLTAVSQLKKGTLRYWLSAGPGDFLLTPAVDANSAFYAAAIDDDFSQCKEGVKA 180
           LRSILIQLT VSQLKKGTLRYWLSAGPGDFLLTPAVDANSAFYA AIDDDFSQCKEGVK 
Sbjct: 121 LRSILIQLTTVSQLKKGTLRYWLSAGPGDFLLTPAVDANSAFYAVAIDDDFSQCKEGVKV 180

Query: 181 ITSTIPMKTPQFATMKNVNYLPNVLAKLEAEEKGAFASIWVDEEGYIAEGPNVNVAFITN 240
           IT+TIPMKTP FATMKNVNYLPNVLAK+EAEEKGAFASIWVDEEGYIAEGPNVNVAFITN
Sbjct: 181 ITTTIPMKTPLFATMKNVNYLPNVLAKMEAEEKGAFASIWVDEEGYIAEGPNVNVAFITN 240

Query: 241 EKELILPSFDKILSGCTALRLLKLAPKLVKEGKLKSVGTANLTVKEAKDAAEMMFVGSTL 300
           EKELILPSFDKILSGCTALRLLKLAPKLVKEGKLKSVGTANLTVKEAK AAEMMFVGSTL
Sbjct: 241 EKELILPSFDKILSGCTALRLLKLAPKLVKEGKLKSVGTANLTVKEAKGAAEMMFVGSTL 300

Query: 301 PLLPIISWDEEPIGDGRVGELTMALSDLLWDDMVSGPETERIPVSYTE 349
           PLLPIISWDEEPIGDG VGELTMALSDLLWDDMVSGPETERIPVSYT+
Sbjct: 301 PLLPIISWDEEPIGDGHVGELTMALSDLLWDDMVSGPETERIPVSYTQ 348

[Close]
BLAST of CSPI04G23190 vs. ExPASy TrEMBL
Match: A0A5D3DWQ3 (Aminotran_1_2 domain-containing protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold384G001560 PE=4 SV=1)

HSP 1 Score: 648.7 bits (1672), Expect = 1.4e-182
Identity = 323/336 (96.13%), Postives = 328/336 (97.62%), Query Frame = 0 

Query: 13  AIGNGSETDLKVPVFSSSLELLEKLHEKWSLVNKKPYPAMYSSVFGGIITDPAMMVIPID 72
           +IGNGSETDLKVPVFSSSLELLEKLHEKWSLV KKPYPAMYSS+ GGIITDPAMMVIPID
Sbjct: 405 SIGNGSETDLKVPVFSSSLELLEKLHEKWSLVEKKPYPAMYSSILGGIITDPAMMVIPID 464

Query: 73  DHMVHRGHGVFDTAIILNGYLYELDAHIDRFLRSASKAKISPPFPRSILRSILIQLTAVS 132
           DHMVHRGHGVFDTAIILNGYLYELDAHIDRFLRSASKAKISPPFPRSILRSILIQLT VS
Sbjct: 465 DHMVHRGHGVFDTAIILNGYLYELDAHIDRFLRSASKAKISPPFPRSILRSILIQLTTVS 524

Query: 133 QLKKGTLRYWLSAGPGDFLLTPAVDANSAFYAAAIDDDFSQCKEGVKAITSTIPMKTPQF 192
           QLKKGTLRYWLSAGPGDFLLTPAVDANSAFYA AIDDDFSQCKEGVK IT+TIPMKTP F
Sbjct: 525 QLKKGTLRYWLSAGPGDFLLTPAVDANSAFYAVAIDDDFSQCKEGVKVITTTIPMKTPLF 584

Query: 193 ATMKNVNYLPNVLAKLEAEEKGAFASIWVDEEGYIAEGPNVNVAFITNEKELILPSFDKI 252
           ATMKNVNYLPNVLAK+EAEEKGAFASIWVDEEGYIAEGPNVNVAFITNEKELILPSFDKI
Sbjct: 585 ATMKNVNYLPNVLAKMEAEEKGAFASIWVDEEGYIAEGPNVNVAFITNEKELILPSFDKI 644

Query: 253 LSGCTALRLLKLAPKLVKEGKLKSVGTANLTVKEAKDAAEMMFVGSTLPLLPIISWDEEP 312
           LSGCTALRLLKLAPKLVKEGKLKSVGTANLTVKEAK AAEMMFVGSTLPLLPIISWDEEP
Sbjct: 645 LSGCTALRLLKLAPKLVKEGKLKSVGTANLTVKEAKGAAEMMFVGSTLPLLPIISWDEEP 704

Query: 313 IGDGRVGELTMALSDLLWDDMVSGPETERIPVSYTE 349
           IGDG VGELTMALSDLLWDDMVSGPETERIPVSYT+
Sbjct: 705 IGDGHVGELTMALSDLLWDDMVSGPETERIPVSYTQ 740

[Close]
BLAST of CSPI04G23190 vs. ExPASy TrEMBL
Match: A0A6J1FBG0 (D-amino-acid transaminase, chloroplastic-like OS=Cucurbita moschata OX=3662 GN=LOC111442546 PE=4 SV=1)

HSP 1 Score: 611.7 bits (1576), Expect = 1.9e-171
Identity = 305/347 (87.90%), Postives = 326/347 (93.95%), Query Frame = 0 

Query: 1   MEALNSSSSEIQAIGNGSETDLKVPVFSSSLELLEKLHEKWSLVNKKPYPAMYSSVFGGI 60
           M A NS S+E  AIGNGSE+DLKV VFSSSLELLEKLHEKWSL  KKPYPAMYSS+FGGI
Sbjct: 1   MAASNSHSNETGAIGNGSESDLKVHVFSSSLELLEKLHEKWSL-KKKPYPAMYSSIFGGI 60

Query: 61  ITDPAMMVIPIDDHMVHRGHGVFDTAIILNGYLYELDAHIDRFLRSASKAKISPPFPRSI 120
           ITDPAMM+IPIDDHMVHRGHGVFDTAIILNG+LYELDAHIDRFLRSA+KAKIS PFPRS+
Sbjct: 61  ITDPAMMMIPIDDHMVHRGHGVFDTAIILNGHLYELDAHIDRFLRSAAKAKISSPFPRSV 120

Query: 121 LRSILIQLTAVSQLKKGTLRYWLSAGPGDFLLTPAVDANSAFYAAAIDDDFSQCKEGVKA 180
           LRSILIQLTAVS+LKKGTLRYWLSAGPGDFLLTP+   NSAFYA AIDDDFSQCKEGVKA
Sbjct: 121 LRSILIQLTAVSELKKGTLRYWLSAGPGDFLLTPSGHGNSAFYAVAIDDDFSQCKEGVKA 180

Query: 181 ITSTIPMKTPQFATMKNVNYLPNVLAKLEAEEKGAFASIWVDEEGYIAEGPNVNVAFITN 240
           ITST+P+KTPQFATMK+VNYLPNVL K+EAEEKGAFASIWVD+EGY+AEGPNVNVAFIT 
Sbjct: 181 ITSTVPIKTPQFATMKSVNYLPNVLTKMEAEEKGAFASIWVDDEGYVAEGPNVNVAFITK 240

Query: 241 EKELILPSFDKILSGCTALRLLKLAPKLVKEGKLKSVGTANLTVKEAKDAAEMMFVGSTL 300
           EKELILP FDK+LSGCTALRLL LAPKLV++ KLKSVGTANLTV+EAKDAAEMMFVGSTL
Sbjct: 241 EKELILPCFDKVLSGCTALRLLALAPKLVEDRKLKSVGTANLTVEEAKDAAEMMFVGSTL 300

Query: 301 PLLPIISWDEEPIGDGRVGELTMALSDLLWDDMVSGPETERIPVSYT 348
           P+LPIISWD+EPIGDGRVGELTMALSDLLWDDMVSG E ERIPV YT
Sbjct: 301 PVLPIISWDDEPIGDGRVGELTMALSDLLWDDMVSGSEIERIPVPYT 346

[Close]
BLAST of CSPI04G23190 vs. ExPASy TrEMBL
Match: A0A6J1IJS3 (D-amino-acid transaminase, chloroplastic-like OS=Cucurbita maxima OX=3661 GN=LOC111476905 PE=4 SV=1)

HSP 1 Score: 610.9 bits (1574), Expect = 3.3e-171
Identity = 305/347 (87.90%), Postives = 324/347 (93.37%), Query Frame = 0 

Query: 1   MEALNSSSSEIQAIGNGSETDLKVPVFSSSLELLEKLHEKWSLVNKKPYPAMYSSVFGGI 60
           M A NS S+E  AIGNG ETDLKV VFSSSLEL EKLHEKWSL  KKPYPAMYSS+FGGI
Sbjct: 1   MAASNSHSNETGAIGNGCETDLKVHVFSSSLELHEKLHEKWSL-KKKPYPAMYSSIFGGI 60

Query: 61  ITDPAMMVIPIDDHMVHRGHGVFDTAIILNGYLYELDAHIDRFLRSASKAKISPPFPRSI 120
           ITDPAMM+IPIDDHMVHRGHGVFDTAIILNG+LYELDAHIDRFLRSA+KAKIS PFPRS+
Sbjct: 61  ITDPAMMMIPIDDHMVHRGHGVFDTAIILNGHLYELDAHIDRFLRSAAKAKISSPFPRSV 120

Query: 121 LRSILIQLTAVSQLKKGTLRYWLSAGPGDFLLTPAVDANSAFYAAAIDDDFSQCKEGVKA 180
           LR ILIQLTAVS+LKKGTLRYWLSAGPGDFLLTP+   NSAFYA AIDDDFSQCKEGVKA
Sbjct: 121 LRHILIQLTAVSELKKGTLRYWLSAGPGDFLLTPSGHGNSAFYAVAIDDDFSQCKEGVKA 180

Query: 181 ITSTIPMKTPQFATMKNVNYLPNVLAKLEAEEKGAFASIWVDEEGYIAEGPNVNVAFITN 240
           ITST+P+KTPQFATMK+VNYLPNVL K+EAEEKGAFASIWVDEEGY+AEGPNVNVAFIT 
Sbjct: 181 ITSTVPIKTPQFATMKSVNYLPNVLTKMEAEEKGAFASIWVDEEGYVAEGPNVNVAFITK 240

Query: 241 EKELILPSFDKILSGCTALRLLKLAPKLVKEGKLKSVGTANLTVKEAKDAAEMMFVGSTL 300
           EKELILP FDK+LSGCTALRLL LAPKLV++GKLKSVGTANLTV+EAKDAAEMMFVGSTL
Sbjct: 241 EKELILPCFDKVLSGCTALRLLALAPKLVEDGKLKSVGTANLTVEEAKDAAEMMFVGSTL 300

Query: 301 PLLPIISWDEEPIGDGRVGELTMALSDLLWDDMVSGPETERIPVSYT 348
           P+LPIISWD+EPIGDGRVGELTMALSDLLWDDMVSG E ERIPV YT
Sbjct: 301 PVLPIISWDDEPIGDGRVGELTMALSDLLWDDMVSGSEIERIPVPYT 346

[Close]
BLAST of CSPI04G23190 vs. NCBI nr
Match: XP_004146044.1 (D-amino-acid transaminase, chloroplastic [Cucumis sativus] >KGN55100.1 hypothetical protein Csa_012285 [Cucumis sativus])

HSP 1 Score: 686.4 bits (1770), Expect = 1.3e-193
Identity = 346/348 (99.43%), Postives = 347/348 (99.71%), Query Frame = 0 

Query: 1   MEALNSSSSEIQAIGNGSETDLKVPVFSSSLELLEKLHEKWSLVNKKPYPAMYSSVFGGI 60
           MEALNSSSSEIQAIGNGSETDLKVPVFSSSLELLEKLHEKWSLVNKKPYPAMYSSVFGGI
Sbjct: 1   MEALNSSSSEIQAIGNGSETDLKVPVFSSSLELLEKLHEKWSLVNKKPYPAMYSSVFGGI 60

Query: 61  ITDPAMMVIPIDDHMVHRGHGVFDTAIILNGYLYELDAHIDRFLRSASKAKISPPFPRSI 120
           ITDPAMMVIPIDDHMVHRGHGVFDTAIILNGYLYELDAHIDRFLRSASKAKISPPFPRSI
Sbjct: 61  ITDPAMMVIPIDDHMVHRGHGVFDTAIILNGYLYELDAHIDRFLRSASKAKISPPFPRSI 120

Query: 121 LRSILIQLTAVSQLKKGTLRYWLSAGPGDFLLTPAVDANSAFYAAAIDDDFSQCKEGVKA 180
           LRSILIQLTAVSQLKKGTLRYWLSAGPGDFLLTPAV+ANSAFYA AIDDDFSQCKEGVKA
Sbjct: 121 LRSILIQLTAVSQLKKGTLRYWLSAGPGDFLLTPAVNANSAFYAVAIDDDFSQCKEGVKA 180

Query: 181 ITSTIPMKTPQFATMKNVNYLPNVLAKLEAEEKGAFASIWVDEEGYIAEGPNVNVAFITN 240
           ITSTIPMKTPQFATMKNVNYLPNVLAKLEAEEKGAFASIWVDEEGYIAEGPNVNVAFITN
Sbjct: 181 ITSTIPMKTPQFATMKNVNYLPNVLAKLEAEEKGAFASIWVDEEGYIAEGPNVNVAFITN 240

Query: 241 EKELILPSFDKILSGCTALRLLKLAPKLVKEGKLKSVGTANLTVKEAKDAAEMMFVGSTL 300
           EKELILPSFDKILSGCTALRLLKLAPKLVKEGKLKSVGTANLTVKEAKDAAEMMFVGSTL
Sbjct: 241 EKELILPSFDKILSGCTALRLLKLAPKLVKEGKLKSVGTANLTVKEAKDAAEMMFVGSTL 300

Query: 301 PLLPIISWDEEPIGDGRVGELTMALSDLLWDDMVSGPETERIPVSYTE 349
           PLLPIISWDEEPIGDGRVGELTMALSDLLWDDMVSGPETERIPVSYTE
Sbjct: 301 PLLPIISWDEEPIGDGRVGELTMALSDLLWDDMVSGPETERIPVSYTE 348

[Close]
BLAST of CSPI04G23190 vs. NCBI nr
Match: XP_008463704.1 (PREDICTED: D-amino-acid transaminase, chloroplastic-like [Cucumis melo])

HSP 1 Score: 669.8 bits (1727), Expect = 1.2e-188
Identity = 336/348 (96.55%), Postives = 340/348 (97.70%), Query Frame = 0 

Query: 1   MEALNSSSSEIQAIGNGSETDLKVPVFSSSLELLEKLHEKWSLVNKKPYPAMYSSVFGGI 60
           MEALNSSSSEIQAIGNGSETDLKVPVFSSSLELLEKLHEKWSLV KKPYPAMYSS+ GGI
Sbjct: 1   MEALNSSSSEIQAIGNGSETDLKVPVFSSSLELLEKLHEKWSLVEKKPYPAMYSSILGGI 60

Query: 61  ITDPAMMVIPIDDHMVHRGHGVFDTAIILNGYLYELDAHIDRFLRSASKAKISPPFPRSI 120
           ITDPAMMVIPIDDHMVHRGHGVFDTAIILNGYLYELDAHIDRFLRSASKAKISPPFPRSI
Sbjct: 61  ITDPAMMVIPIDDHMVHRGHGVFDTAIILNGYLYELDAHIDRFLRSASKAKISPPFPRSI 120

Query: 121 LRSILIQLTAVSQLKKGTLRYWLSAGPGDFLLTPAVDANSAFYAAAIDDDFSQCKEGVKA 180
           LRSILIQLT VSQLKKGTLRYWLSAGPGDFLLTPAVDANSAFYA AIDDDFSQCKEGVK 
Sbjct: 121 LRSILIQLTTVSQLKKGTLRYWLSAGPGDFLLTPAVDANSAFYAVAIDDDFSQCKEGVKV 180

Query: 181 ITSTIPMKTPQFATMKNVNYLPNVLAKLEAEEKGAFASIWVDEEGYIAEGPNVNVAFITN 240
           IT+TIPMKTP FATMKNVNYLPNVLAK+EAEEKGAFASIWVDEEGYIAEGPNVNVAFITN
Sbjct: 181 ITTTIPMKTPLFATMKNVNYLPNVLAKMEAEEKGAFASIWVDEEGYIAEGPNVNVAFITN 240

Query: 241 EKELILPSFDKILSGCTALRLLKLAPKLVKEGKLKSVGTANLTVKEAKDAAEMMFVGSTL 300
           EKELILPSFDKILSGCTALRLLKLAPKLVKEGKLKSVGTANLTVKEAK AAEMMFVGSTL
Sbjct: 241 EKELILPSFDKILSGCTALRLLKLAPKLVKEGKLKSVGTANLTVKEAKGAAEMMFVGSTL 300

Query: 301 PLLPIISWDEEPIGDGRVGELTMALSDLLWDDMVSGPETERIPVSYTE 349
           PLLPIISWDEEPIGDG VGELTMALSDLLWDDMVSGPETERIPVSYT+
Sbjct: 301 PLLPIISWDEEPIGDGHVGELTMALSDLLWDDMVSGPETERIPVSYTQ 348

[Close]
BLAST of CSPI04G23190 vs. NCBI nr
Match: KAA0066805.1 (hypothetical protein E6C27_scaffold271G001630 [Cucumis melo var. makuwa] >TYK27952.1 hypothetical protein E5676_scaffold384G001560 [Cucumis melo var. makuwa])

HSP 1 Score: 648.7 bits (1672), Expect = 2.9e-182
Identity = 323/336 (96.13%), Postives = 328/336 (97.62%), Query Frame = 0 

Query: 13  AIGNGSETDLKVPVFSSSLELLEKLHEKWSLVNKKPYPAMYSSVFGGIITDPAMMVIPID 72
           +IGNGSETDLKVPVFSSSLELLEKLHEKWSLV KKPYPAMYSS+ GGIITDPAMMVIPID
Sbjct: 405 SIGNGSETDLKVPVFSSSLELLEKLHEKWSLVEKKPYPAMYSSILGGIITDPAMMVIPID 464

Query: 73  DHMVHRGHGVFDTAIILNGYLYELDAHIDRFLRSASKAKISPPFPRSILRSILIQLTAVS 132
           DHMVHRGHGVFDTAIILNGYLYELDAHIDRFLRSASKAKISPPFPRSILRSILIQLT VS
Sbjct: 465 DHMVHRGHGVFDTAIILNGYLYELDAHIDRFLRSASKAKISPPFPRSILRSILIQLTTVS 524

Query: 133 QLKKGTLRYWLSAGPGDFLLTPAVDANSAFYAAAIDDDFSQCKEGVKAITSTIPMKTPQF 192
           QLKKGTLRYWLSAGPGDFLLTPAVDANSAFYA AIDDDFSQCKEGVK IT+TIPMKTP F
Sbjct: 525 QLKKGTLRYWLSAGPGDFLLTPAVDANSAFYAVAIDDDFSQCKEGVKVITTTIPMKTPLF 584

Query: 193 ATMKNVNYLPNVLAKLEAEEKGAFASIWVDEEGYIAEGPNVNVAFITNEKELILPSFDKI 252
           ATMKNVNYLPNVLAK+EAEEKGAFASIWVDEEGYIAEGPNVNVAFITNEKELILPSFDKI
Sbjct: 585 ATMKNVNYLPNVLAKMEAEEKGAFASIWVDEEGYIAEGPNVNVAFITNEKELILPSFDKI 644

Query: 253 LSGCTALRLLKLAPKLVKEGKLKSVGTANLTVKEAKDAAEMMFVGSTLPLLPIISWDEEP 312
           LSGCTALRLLKLAPKLVKEGKLKSVGTANLTVKEAK AAEMMFVGSTLPLLPIISWDEEP
Sbjct: 645 LSGCTALRLLKLAPKLVKEGKLKSVGTANLTVKEAKGAAEMMFVGSTLPLLPIISWDEEP 704

Query: 313 IGDGRVGELTMALSDLLWDDMVSGPETERIPVSYTE 349
           IGDG VGELTMALSDLLWDDMVSGPETERIPVSYT+
Sbjct: 705 IGDGHVGELTMALSDLLWDDMVSGPETERIPVSYTQ 740

[Close]
BLAST of CSPI04G23190 vs. NCBI nr
Match: XP_038900252.1 (D-amino-acid transaminase, chloroplastic-like isoform X2 [Benincasa hispida])

HSP 1 Score: 646.7 bits (1667), Expect = 1.1e-181
Identity = 322/348 (92.53%), Postives = 335/348 (96.26%), Query Frame = 0 

Query: 1   MEALNSSSSEIQAIGNGSETDLKVPVFSSSLELLEKLHEKWSLVNKKPYPAMYSSVFGGI 60
           MEALNS S+E QAIGNGSETDLKV VFSSS ELLEKLH+KWSLV KKPYPAMYSS+FGGI
Sbjct: 1   MEALNSPSNETQAIGNGSETDLKVHVFSSSFELLEKLHQKWSLVKKKPYPAMYSSIFGGI 60

Query: 61  ITDPAMMVIPIDDHMVHRGHGVFDTAIILNGYLYELDAHIDRFLRSASKAKISPPFPRSI 120
           ITDPAMMVIPIDDHMVHRGHGVFDTAIILNG+LYELDAH+DRFLRSASKAKIS PFPRSI
Sbjct: 61  ITDPAMMVIPIDDHMVHRGHGVFDTAIILNGHLYELDAHLDRFLRSASKAKISSPFPRSI 120

Query: 121 LRSILIQLTAVSQLKKGTLRYWLSAGPGDFLLTPAVDANSAFYAAAIDDDFSQCKEGVKA 180
           LRSILIQLTAVSQLKKGTLRYWLSAGPGDFLLTPAV+A SAFYA AIDDDFSQCKEGVKA
Sbjct: 121 LRSILIQLTAVSQLKKGTLRYWLSAGPGDFLLTPAVNAKSAFYAVAIDDDFSQCKEGVKA 180

Query: 181 ITSTIPMKTPQFATMKNVNYLPNVLAKLEAEEKGAFASIWVDEEGYIAEGPNVNVAFITN 240
           IT+T+PMKTPQFATMKNVNYLPNVLAKLEAEEKGAFASIWVDEEGYIAEGPNVNVAFITN
Sbjct: 181 ITTTVPMKTPQFATMKNVNYLPNVLAKLEAEEKGAFASIWVDEEGYIAEGPNVNVAFITN 240

Query: 241 EKELILPSFDKILSGCTALRLLKLAPKLVKEGKLKSVGTANLTVKEAKDAAEMMFVGSTL 300
           EKELI PSFDK+LSGCTALRLLK+APKLV++GKLKSVGTANLTVKEAK AAEMMFVGSTL
Sbjct: 241 EKELIFPSFDKVLSGCTALRLLKIAPKLVEDGKLKSVGTANLTVKEAKGAAEMMFVGSTL 300

Query: 301 PLLPIISWDEEPIGDGRVGELTMALSDLLWDDMVSGPETERIPVSYTE 349
           PLLPIISWD EPIGDG VGELTMALSDLLWDDMVSGPETERIPV YT+
Sbjct: 301 PLLPIISWDGEPIGDGSVGELTMALSDLLWDDMVSGPETERIPVPYTQ 348

[Close]
BLAST of CSPI04G23190 vs. NCBI nr
Match: XP_038900251.1 (D-amino-acid transaminase, chloroplastic-like isoform X1 [Benincasa hispida])

HSP 1 Score: 631.7 bits (1628), Expect = 3.7e-177
Identity = 313/336 (93.15%), Postives = 325/336 (96.73%), Query Frame = 0 

Query: 13  AIGNGSETDLKVPVFSSSLELLEKLHEKWSLVNKKPYPAMYSSVFGGIITDPAMMVIPID 72
           AIGNGSETDLKV VFSSS ELLEKLH+KWSLV KKPYPAMYSS+FGGIITDPAMMVIPID
Sbjct: 80  AIGNGSETDLKVHVFSSSFELLEKLHQKWSLVKKKPYPAMYSSIFGGIITDPAMMVIPID 139

Query: 73  DHMVHRGHGVFDTAIILNGYLYELDAHIDRFLRSASKAKISPPFPRSILRSILIQLTAVS 132
           DHMVHRGHGVFDTAIILNG+LYELDAH+DRFLRSASKAKIS PFPRSILRSILIQLTAVS
Sbjct: 140 DHMVHRGHGVFDTAIILNGHLYELDAHLDRFLRSASKAKISSPFPRSILRSILIQLTAVS 199

Query: 133 QLKKGTLRYWLSAGPGDFLLTPAVDANSAFYAAAIDDDFSQCKEGVKAITSTIPMKTPQF 192
           QLKKGTLRYWLSAGPGDFLLTPAV+A SAFYA AIDDDFSQCKEGVKAIT+T+PMKTPQF
Sbjct: 200 QLKKGTLRYWLSAGPGDFLLTPAVNAKSAFYAVAIDDDFSQCKEGVKAITTTVPMKTPQF 259

Query: 193 ATMKNVNYLPNVLAKLEAEEKGAFASIWVDEEGYIAEGPNVNVAFITNEKELILPSFDKI 252
           ATMKNVNYLPNVLAKLEAEEKGAFASIWVDEEGYIAEGPNVNVAFITNEKELI PSFDK+
Sbjct: 260 ATMKNVNYLPNVLAKLEAEEKGAFASIWVDEEGYIAEGPNVNVAFITNEKELIFPSFDKV 319

Query: 253 LSGCTALRLLKLAPKLVKEGKLKSVGTANLTVKEAKDAAEMMFVGSTLPLLPIISWDEEP 312
           LSGCTALRLLK+APKLV++GKLKSVGTANLTVKEAK AAEMMFVGSTLPLLPIISWD EP
Sbjct: 320 LSGCTALRLLKIAPKLVEDGKLKSVGTANLTVKEAKGAAEMMFVGSTLPLLPIISWDGEP 379

Query: 313 IGDGRVGELTMALSDLLWDDMVSGPETERIPVSYTE 349
           IGDG VGELTMALSDLLWDDMVSGPETERIPV YT+
Sbjct: 380 IGDGSVGELTMALSDLLWDDMVSGPETERIPVPYTQ 415

[Close]
BLAST of CSPI04G23190 vs. TAIR 10
Match: AT5G57850.1 (D-aminoacid aminotransferase-like PLP-dependent enzymes superfamily protein )

HSP 1 Score: 362.1 bits (928), Expect = 5.1e-100
Identity = 186/332 (56.02%), Postives = 240/332 (72.29%), Query Frame = 0 

Query: 16  NGSETDLKVPVFSSSLELLEKLHEKWSLVNKKPYPAMYSSVFGGIITDPAMMVIPIDDHM 75
           + S     VPV  SS E+ E+L         + + AMYSSV  GI TDPA MV+P+DDHM
Sbjct: 46  DSSSQSWNVPVL-SSYEVGERLK---LARGGQQFLAMYSSVVDGITTDPAAMVLPLDDHM 105

Query: 76  VHRGHGVFDTAIILNGYLYELDAHIDRFLRSASKAKISPPFPRSILRSILIQLTAVSQLK 135
           VHRGHGVFDTA+I+NGYLYELD H+DR LRSAS AKI  PF R  ++ ILIQ  +VS  +
Sbjct: 106 VHRGHGVFDTALIINGYLYELDQHLDRILRSASMAKIPLPFDRETIKRILIQTVSVSGCR 165

Query: 136 KGTLRYWLSAGPGDFLLTPAVDANSAFYAAAIDDDFSQCKEGVKAITSTIPMKTPQFATM 195
            G+LRYWLSAGPGDFLL+P+       YA  I  +F+    GVK +TS+IP+K P+FAT+
Sbjct: 166 DGSLRYWLSAGPGDFLLSPSQCLKPTLYAIVIKTNFAINPIGVKVVTSSIPIKPPEFATV 225

Query: 196 KNVNYLPNVLAKLEAEEKGAFASIWVDEEGYIAEGPNVNVAFITN-EKELILPSFDKILS 255
           K+VNYLPNVL+++EAE KGA+A IWV ++G+IAEGPN+NVAF+ N  KEL++P FD +LS
Sbjct: 226 KSVNYLPNVLSQMEAEAKGAYAGIWVCKDGFIAEGPNMNVAFVVNGGKELVMPRFDNVLS 285

Query: 256 GCTALRLLKLAPKLVKEGKLKSVGTANLTVKEAKDAAEMMFVGSTLPLLPIISWDEEPIG 315
           GCTA R L LA +LV +G LK+V   ++TV++ K A EMM +GS +P+ P+I WDEE IG
Sbjct: 286 GCTAKRTLTLAEQLVSKGILKTVKVMDVTVEDGKKADEMMLIGSGIPIRPVIQWDEEFIG 345

Query: 316 DGRVGELTMALSDLLWDDMVSGPETERIPVSY 347
           +G+ G +  AL DLL +DM SGP + R+ V Y
Sbjct: 346 EGKEGPIAKALLDLLLEDMRSGPPSVRVLVPY 373

[Close]
BLAST of CSPI04G23190 vs. TAIR 10
Match: AT3G05190.1 (D-aminoacid aminotransferase-like PLP-dependent enzymes superfamily protein )

HSP 1 Score: 74.3 bits (181), Expect = 2.1e-13
Identity = 69/276 (25.00%), Postives = 125/276 (45.29%), Query Frame = 0 

Query: 58  GGIITDPAMMVIPIDDHMVHRGHGVFDTAIILNGYLYELDAHIDRFLRSA-SKAKISPPF 117
           G  I    M  + + D +V  G  V++   I  G +++L+ H+DR   SA + A  + P 
Sbjct: 267 GDEILPREMAKVSVFDSVVQGGDSVWEGLRIYKGKIFKLEEHLDRLFDSAKALAFDNVPA 326

Query: 118 PRSILRSILIQLTAVSQLKKGTLRYWLSAGPG-DFLLTPAVD--ANSAFYAAAIDDDFSQ 177
              +  +I   L          +R  L+ G      ++PA +    +    A        
Sbjct: 327 REEVKEAIFRTLITNGMFDNTHIRLSLTRGKKVTSGMSPAYNRYGCTLIVLAEWKPPVYD 386

Query: 178 CKEGVKAITSTIPMKTPQFATMK--NVNYLPNVLAKLEAEEKGAFASIWVDEEGYIAEGP 237
            + G+  +T+T    +P     K  + N L N+LAK+E+    A  +I +D++GY++E  
Sbjct: 387 NEGGIVLVTATTRRNSPNNLDSKIHHNNLLNNILAKIESNNTNAADAIMLDKDGYVSETN 446

Query: 238 NVNVAFITNEKELILPSFDKILSGCTALRLLKLAPKLVKEGKLKSVGTANLTVKEAKDAA 297
             N+ F+  +  ++ P  D  L G T   +++L   +VKE  +  +    +++ E   A 
Sbjct: 447 ATNI-FMVKKGCVLTPHADYCLPGITRATVMEL---VVKENFI--LEERRISLSEFHTAN 506

Query: 298 EMMFVGSTLPLLPIISWDEEPIGDGRVGELTMALSD 328
           E+   G+   L P++  D   IGDG+VG +T  L +
Sbjct: 507 EVWTTGTMGELSPVVKIDGRVIGDGKVGPVTRTLQN 536

[Close]
BLAST of CSPI04G23190 vs. TAIR 10
Match: AT5G27410.1 (D-aminoacid aminotransferase-like PLP-dependent enzymes superfamily protein )

HSP 1 Score: 69.7 bits (169), Expect = 5.2e-12
Identity = 67/276 (24.28%), Postives = 123/276 (44.57%), Query Frame = 0 

Query: 58  GGIITDPAMMVIPIDDHMVHRGHGVFDTAIILNGYLYELDAHIDRFLRSA-SKAKISPPF 117
           G  I    M  + + D +V  G  V++   I  G +++L+ H+DR   SA + A  + P 
Sbjct: 264 GDEIVPREMAKVSVFDSVVQGGDSVWEGLRIYKGKVFKLEEHLDRLSDSAKALAFNNVPT 323

Query: 118 PRSILRSILIQLTAVSQLKKGTLRYWLSAGPG-DFLLTPAVD--ANSAFYAAAIDDDFSQ 177
              I  +I   L          +R  L+ G      ++PA +    +    A        
Sbjct: 324 REEIKEAIFRTLITNGMFDNTHIRLSLTRGKKVTSGMSPAFNRYGCTLIVLAEWKPPVYD 383

Query: 178 CKEGVKAITSTIPMKTPQFATMK--NVNYLPNVLAKLEAEEKGAFASIWVDEEGYIAEGP 237
              G+  +T+T    +P     K  + N L N+LAK+E+       +I +D++G+++E  
Sbjct: 384 NDGGIVLVTATTRRNSPNNLDSKIHHNNLLNNILAKIESNNANVDDAIMLDKDGFVSETN 443

Query: 238 NVNVAFITNEKELILPSFDKILSGCTALRLLKLAPKLVKEGKLKSVGTANLTVKEAKDAA 297
             N+ F+  +  ++ P  D  L G T   +++L   +VKE  +  +    +++ E   A 
Sbjct: 444 ATNI-FMVKKDRVLTPHADYCLPGITRATVMEL---VVKENFI--LEERRISLSEFHTAD 503

Query: 298 EMMFVGSTLPLLPIISWDEEPIGDGRVGELTMALSD 328
           E+   G+   L P++  D   IG+G+VG +T  L +
Sbjct: 504 EVWTTGTMGELSPVVKIDGRVIGEGKVGPVTRRLQN 533

[Close]
BLAST of CSPI04G23190 vs. TAIR 10
Match: AT5G27410.2 (D-aminoacid aminotransferase-like PLP-dependent enzymes superfamily protein )

HSP 1 Score: 69.7 bits (169), Expect = 5.2e-12
Identity = 67/276 (24.28%), Postives = 123/276 (44.57%), Query Frame = 0 

Query: 58  GGIITDPAMMVIPIDDHMVHRGHGVFDTAIILNGYLYELDAHIDRFLRSA-SKAKISPPF 117
           G  I    M  + + D +V  G  V++   I  G +++L+ H+DR   SA + A  + P 
Sbjct: 641 GDEIVPREMAKVSVFDSVVQGGDSVWEGLRIYKGKVFKLEEHLDRLSDSAKALAFNNVPT 700

Query: 118 PRSILRSILIQLTAVSQLKKGTLRYWLSAGPG-DFLLTPAVD--ANSAFYAAAIDDDFSQ 177
              I  +I   L          +R  L+ G      ++PA +    +    A        
Sbjct: 701 REEIKEAIFRTLITNGMFDNTHIRLSLTRGKKVTSGMSPAFNRYGCTLIVLAEWKPPVYD 760

Query: 178 CKEGVKAITSTIPMKTPQFATMK--NVNYLPNVLAKLEAEEKGAFASIWVDEEGYIAEGP 237
              G+  +T+T    +P     K  + N L N+LAK+E+       +I +D++G+++E  
Sbjct: 761 NDGGIVLVTATTRRNSPNNLDSKIHHNNLLNNILAKIESNNANVDDAIMLDKDGFVSETN 820

Query: 238 NVNVAFITNEKELILPSFDKILSGCTALRLLKLAPKLVKEGKLKSVGTANLTVKEAKDAA 297
             N+ F+  +  ++ P  D  L G T   +++L   +VKE  +  +    +++ E   A 
Sbjct: 821 ATNI-FMVKKDRVLTPHADYCLPGITRATVMEL---VVKENFI--LEERRISLSEFHTAD 880

Query: 298 EMMFVGSTLPLLPIISWDEEPIGDGRVGELTMALSD 328
           E+   G+   L P++  D   IG+G+VG +T  L +
Sbjct: 881 EVWTTGTMGELSPVVKIDGRVIGEGKVGPVTRRLQN 910

The following BLAST results are available for this feature:
BLAST of CSPI04G23190 vs. ExPASy Swiss-Prot
Match NameE-valueIdentityDescription
Q8L4937.2e-9956.02D-amino-acid transaminase, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=DAAT... [more]
Q584143.5e-2127.17Putative branched-chain-amino-acid aminotransferase OS=Methanocaldococcus jannas... [more]
O293296.7e-2026.76Putative branched-chain-amino-acid aminotransferase OS=Archaeoglobus fulgidus (s... [more]
O075975.8e-1626.25D-alanine aminotransferase OS=Bacillus subtilis (strain 168) OX=224308 GN=dat PE... [more]
P635111.6e-1325.37D-alanine aminotransferase OS=Staphylococcus aureus (strain Mu50 / ATCC 700699) ... [more]
BLAST of CSPI04G23190 vs. ExPASy TrEMBL
Match NameE-valueIdentityDescription
A0A0A0KZK66.2e-19499.43Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_4G629460 PE=4 SV=1[more]
A0A1S3CJV56.0e-18996.55D-amino-acid transaminase, chloroplastic-like OS=Cucumis melo OX=3656 GN=LOC1035... [more]
A0A5D3DWQ31.4e-18296.13Aminotran_1_2 domain-containing protein OS=Cucumis melo var. makuwa OX=1194695 G... [more]
A0A6J1FBG01.9e-17187.90D-amino-acid transaminase, chloroplastic-like OS=Cucurbita moschata OX=3662 GN=L... [more]
A0A6J1IJS33.3e-17187.90D-amino-acid transaminase, chloroplastic-like OS=Cucurbita maxima OX=3661 GN=LOC... [more]
BLAST of CSPI04G23190 vs. NCBI nr
Match NameE-valueIdentityDescription
XP_004146044.11.3e-19399.43D-amino-acid transaminase, chloroplastic [Cucumis sativus] >KGN55100.1 hypotheti... [more]
XP_008463704.11.2e-18896.55PREDICTED: D-amino-acid transaminase, chloroplastic-like [Cucumis melo][more]
KAA0066805.12.9e-18296.13hypothetical protein E6C27_scaffold271G001630 [Cucumis melo var. makuwa] >TYK279... [more]
XP_038900252.11.1e-18192.53D-amino-acid transaminase, chloroplastic-like isoform X2 [Benincasa hispida][more]
XP_038900251.13.7e-17793.15D-amino-acid transaminase, chloroplastic-like isoform X1 [Benincasa hispida][more]
BLAST of CSPI04G23190 vs. TAIR 10
Match NameE-valueIdentityDescription
AT5G57850.15.1e-10056.02D-aminoacid aminotransferase-like PLP-dependent enzymes superfamily protein [more]
AT3G05190.12.1e-1325.00D-aminoacid aminotransferase-like PLP-dependent enzymes superfamily protein [more]
AT5G27410.15.2e-1224.28D-aminoacid aminotransferase-like PLP-dependent enzymes superfamily protein [more]
AT5G27410.25.2e-1224.28D-aminoacid aminotransferase-like PLP-dependent enzymes superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (PI 183967) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001544Aminotransferase class IVPFAMPF01063Aminotran_4coord: 81..308
e-value: 2.7E-41
score: 141.8
IPR043132Branched-chain-amino-acid aminotransferase-like, C-terminalGENE3D3.20.10.10coord: 170..338
e-value: 1.4E-35
score: 124.2
IPR043131Branched-chain-amino-acid aminotransferase-like, N-terminalGENE3D3.30.470.10coord: 23..166
e-value: 1.6E-22
score: 81.9
NoneNo IPR availablePANTHERPTHR42743:SF8BRANCHED-CHAIN AMINO ACID AMINOTRANSFERASE-LIKEcoord: 6..345
NoneNo IPR availablePANTHERPTHR42743AMINO-ACID AMINOTRANSFERASEcoord: 6..345
IPR017824Aminodeoxychorismate lyase, class IVCDDcd01559ADCL_likecoord: 73..329
e-value: 2.69458E-71
score: 220.644
IPR036038Aminotransferase-like, PLP-dependent enzymesSUPERFAMILY56752D-aminoacid aminotransferase-like PLP-dependent enzymescoord: 56..334

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CSPI04G23190.1CSPI04G23190.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006520 cellular amino acid metabolic process
biological_process GO:0046656 folic acid biosynthetic process
molecular_function GO:0008696 4-amino-4-deoxychorismate lyase activity
molecular_function GO:0030170 pyridoxal phosphate binding
molecular_function GO:0008483 transaminase activity
molecular_function GO:0003824 catalytic activity