CSPI04G21060 (gene) Cucumber (PI 183967) v1

Overview
NameCSPI04G21060
Typegene
OrganismCucumis sativus var. hardwickii cv. PI 183967 (Cucumber (PI 183967) v1)
DescriptionSerine/threonine protein kinase
LocationChr4: 19489224 .. 19494834 (-)
RNA-Seq ExpressionCSPI04G21060
SyntenyCSPI04G21060
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
AAAAAAGCATAGCCAGAAAACTACAAAAGTTCATTTATTTTTTACCAAAAAAATTCATCAATTTGGATGTGGGTATAGACCAAAATCAGATTTTATTAATTCAGAATGGAAAAACGTTTGTGAGAAAGAAAGAGGAGAGAGAAAAACGGCTCATTCTTCTCAAAATCCTTACTACCTACGCATCTGTTACTGAGGTTGCAGCAGAAAGGGGGAAAAAAAGTGGAGCAAAACAGTTGAAATTTCATTCTGGAAGTGGGAATTTCGGTCATTACCAATTTCTTCCAAAAGGGTAAGATTTTCTTTCAAGTGGGGTTGTTGACATCTCAACTTCTTGGCTGTAAAACATTCCATTCTCTGAATTTGAAGCTCTCATGAATCTGTAAATGGAAGCAAAGCTTTTGGGTTTGCCTTTCTTTTTCATCTTCCTAACTTCCCTCTTTACCTTTTTCTTTCTCTTGATTAAGAAGCTTTTGTTTATGGTTGATGCTTACTTTGCTCGTTTATGTTTGTTTTTCTTCTCTCTCTTGTTTTAGCAAGTTATCATTTCGCTTTTATTATGCTATTTAGTACTGTCTTGCTTTTCTCTTGAATTTATCATTCCACCATTCCCCTTCACGTTCGTTGGAATATCATCATAATTGCACTTGCTTTACTCATTTGTTGTGTTTTCCTTCACACATGTCTCCGTAGATTCTGGAACTTCTGCTTATAATTCAAAGGGGTTATATTTTATGTGGGAGATTTTTGTCTCGACCTTTATCACCTTGGTGTAGGATTTTCATATTTGTTAAACGTCTTAACCATGTGAGAGCACTGCAAAATAGTTTCAGTTTCAGTTGATTTTGGAACTTGGCTCTGCCCCATAGGGGTTTCTGGTCTCACACTGTGCTTTTTGGCGTTTGAAGGTCGACATGGAGAGTGATAATGGAGATATGAAGACCAGGATGGAAGACTATGAAGTTATTGAGCAGATTGGGAGGGGGGCATTTGGATCGGCTTTTCTTGTGTATCACAAGACTGAGAAGAAGAAGTAAGAAATATTTTTGAACTTCTGTTATTTCAGCAACTGCTTCTAAGTCCTTCAAATGAAAACCCTGATGTTCACACTTCCTTGTAATTATTGTGAGAAATGCTGATGCTTAGCTGTGTATTAAGTTGAGATCTATGGTTAGTTTTTTGTTCTTCTCAGTTGCTAATGGAACCCTAATCTTCATAACTAAAATGGCCCATTCAAGATTTATACCTTTTTTTTTCTGAGTGTAGAAGTGAAACAATTTTTTGGTCTTGTGGCATCACAAAATTGTTCGGCTTATTTTAGTGCTTCTCTCAATATTTACGTTCTTCCTAAATAGATATGTACTCAAGAAAATGATCATGGTTAGTTTTCTATTGTTCTCTGTTGCTAATGGAACCCCAATCGTCATAACTAAAATGGCCCATTCAAGATTTATACCTTTTTTTCTGAGTGTAGAAATGAAAAAGATTTTTGCTCTTTTGGCATCACACAATTGTTCGGCTTATTTTAGTGCTTCTCTCAATATTACCTTCTTTCTGAATAGATATGTACTCAAGAAAATTCGCTTGGCAAAGCAAACAGAGAAGTTCAAGCGCACTGCCCACCAAGAGGTTAAGTTTATTTAGAACCTATATCAACTATTTAACTGTTTGAAATACTGCTCATTATTCTCAAGATCTGATTTAATTGACTCTGTTCTGTCTTTACAATTTGTCAACTACAAATGAATTGCTAGTTAACAGGGGGTAAATAAATAGCTATTAACTTAGGAAATGTTTAATGTCTGCCTCACGTTCAACGATAATGCTTAATGATACAAAACAGATCACATAGAAATTGGAGAAAATATAAAGAATTTTATGTTGGCAAATGTGGGAAGGGTAAATTATGGAAGATTGAGTGTTTTCTTGATTCTATGATACGACTCCATATGTTCTTTATTATGAAATGAATTGAGCCATTGTGTACTTCTTCAGTACTTTTTACTTCTCTAGAACAGTCTAACCAGGTGACTTGAAAGTTTTGTGGAAAATTAGTGACTTCTTTATTTTTTCCTGAATTTAGTAGATAATAGGTTTTTAAAAGGAATTTGCTTTAAAATAAAATGCATTCAAAGTTTGTAGACATGGCAAACTCTTAAATCATCAATTCAACCAAAAGCTTAAGCGGGTGGTTGAAGGCAAATTTCATTATATATCACAACACTCCCCCTCACTTGTGGGCTTGAAATATTTGAAACGCCCAACAAGTGAAAATCAATTTTAATTGGGGAGGAAACAACAATTAGGACCTTTTGGACCACCTGCATTGGTACCAGCTCATATTACCAATTCAACCAAAAGCTTAAACTAATGGTTGAAGGCAAAATTAATTATATATAACTAACACAAACTATCTCACTAATCTTGATTTACAACTAAGAAGATACCTTAGTTTGGTACAATGGTTAAAGGGGCAAGTTGAACACCTAGACGCTGAGGTCATTTTTCTAACTATGGTGCTCTGGATGGCATATGTTGTTGGGTTAAGGGGTTCTCCTAAATGATAAGTCAAGTGTGCTTAGGCGGGTTCAGACGCCCAAAAAAATGGACATTTTGTTTTCATCCTCAGTGTGAACTTGGCATGTTTTCTCTTCAAAATTTAACTTAACGTAGTTGATGAGTAAATATCCTTGATATTCACAGTATGTTTCGTTACTGATGTGACCTTGTCAATTACAGATGAATTTGATTGCAAAACTAAATAACCCATATATTGTGGACTACAAAGATTCATGGGTGGATAAGGTAAATTTTGTTCTTTCCCCTCCTATTCAGATCTGGATTGAATCTGTTTCTTTTACAATAAGAACTCTTGCTTTTAGAATTTGGAAGTAGAGGTTGGTTTTTGTTTTCTCAGTTCTTATGTGGATTTTTCTTTTTCGAGCCTACCTTGTGTTGTGTATAATCTTGATCCTTCATGCTGAAAGATTGTAGTTTAAGATATTTTAGATTGTTGGGTCAATATTGCTTTGGATAAGACACAATTTCATTGATGAATGGAATATTAAATGAGTTCTGTTAAAAAGAGATTATTTAATACAAAAGTTGAATCAATACTGTTAAAAAGGATTATTTAATACAAAAGGGGTGATTGTGACTACTTTCTTTCTTTGGAAGGAAATGAATTGTTTATTTCTGAGTGACTCAAAGAGAAAAGTGGATTCTCAATTTGAGAGGGTGTTCGCAATCGACTATAAATATATGGTCTATAGATCATAGCTAAAGCTATGGTGATTCTTTGTATTCTCATTTTCACTCACGTTTCATATTTTGGGCATTCTGTTTTGTAGGGTGATTGTATATGCATTGTAACTGGCTACTGTGAAGGAGGAGACATGTAAGTGTCTAAGTGATAGGTATTTAATACCATTTAGAGTTTTCTTTATTTACAACCTTTACAGAGTAGTTTATTTTTTGGTTCAGGTCATGATTAAATTTTAGATTGAATGAATAATCTGCAACCTTTTTAACAATGTTTATTGATTCCCCAGCTCTAAAGAAACAGAACTAATTGAAGAATACATGTTGTCTTGAATTAATAAGAGAACAAACGTTAGGTGGTCCACTGCTAGTCATGCGTTCAATTTATTTTGCTTTCTCAGGATCCTTCATTTCTAATTTCTAAATGTCCTCCACTACCATCCTCTTTCTTCCTTCTCTGATGTGTATATCTGACCAGGTTAAAAATCTGATTGAAAACCCCTTCCATTGATATAAATAAAAAAGGATTTGGCTAAAAAATTCTCCCTCACCTTCTTTCCTTCCCTCATTTTAATCTCTCCCTTCGAGATTTCATCTTCTATTAATGTCATTGACAGCATGAATCTAAGTGATGAAGATTAGGTATCCGAAGAAGAGATTGGTGAATGATAATAAAAGATCCTTCCAGGTGGCAAAGATTAAAAAAACAAAGCATGAAATTCTTTGGTGACAAAAAGTCAACATTAGTCTTATTTCACGACATATTATTGTAGAAATATATGAGTCGATATGAGTGTTTTCTGCTGTAGAGAAAGAGAGAAAGGGATGAAAAGAAAAATCAAAACTAATGGTACGCCTTGTTTTCTTTTGTTGAACAGGTCTGAACTGATCAAAAAGGCCAGAGGAACATATTTTCCGGAGGAAGTAAGTCGATTTCAGTGTTAATTATTCTTTTCCTACCAAACCTATGTCTGATTGCCATTTCGTTCTCTCAGAAACTTTGCAAGTGGTTGGCACAGTTGTTGCTAGCTGTGGACTATCTGCACTCCAATCGAGTACTACATAGAGATCTTAAGGTATTTAATTAGTATTAAGTATTAACCTACACAATATTGCAATTAAAAACTCATTAATGATACTGATCTGTTTTTTATTTTCAAATTTTCTTCAATTACAGTGTTCCAACATTTTCCTTACAAAGGGCAATGATATCCGGCTTGGTATGATGTCTTCCAATTTAACTTGGATGGGATTTCTTTTCCTAGTCATTTCTTCTAGAAAAGTGCTAATATCTAGGATGTCTGTACAGGGGACTTCGGACTTGCAAAACTGCTGAATACAGAAGACCTTGCTTCATCGGTAAGATGCAATCATTGTCTGTGGTAATTTTGTTATAGCAAGCTTTTGAATCAACAAATTTATATTTTATTGTAATAAAACCTGGTAAGGTCAAGGCCATGGAATGCTTAACTTCTGCCACGTAAACTATGATCCGGAGGTTTTAAAATGGTTGTTTATTTACCTTTGAATTCATGTCAGTAACTTCTTCATTAGGTTTTATTCCACGCAATTTCTTTTATGTTGCCAATATTCATGGTATTGTGCTTTCTTTCCTTGAGAATGTCGAGCATTGAGAACACATGGTTGAGGTTCCCTTCCCTCTGTTTCTCCCTTGGCACATTCACACCACTGGATCAATTGATCCCACCAAGCCCACAATTTTGGCCACATAACTACAAACTGCCAAAAAAAAATTCCTTGAAAATTATGCATTGTGCCATCATAGATGTCGTGTCGGGATAAAATAGTAATGAGTAGCTCAATATTGTTGGGAGAGATCTGGATCACTTAGATGTCTAAACTATAATTCTTTCACCTATCTATTAATGGGTATATTTTAGGTAAGGTTCTATCAATGAATACTGGTAGAATTAGTGGGCTTTGGTCCAAGGAAAGATTTACCCTAATTTTCTTTCCTTTTTTATCATAAGCTTGTTGTCTATTTATTTTCCCCCTCGTATTTTTTTGTTAATATGAGGAAATAATAAGAAAGTCAGATCATGGTTTTTCTCTCGATACTCGGATTTTCATGTAACTCAGTGTCTATTTTCTTTTACTGCTTCTAATATGGTATTAGAGCTAGGTAAAGATGAAACCTTAGACATCAACCTTGATGAAACCCAAATTGTTCACAAGACAGTCACCGGGATCAATGTCGCTGCTGCTGCCGCTATGGAAAAATTACTCCAATAACTTCAGACGCCGCCAATGAGCAATTCTGTCTCACCCTCGGAGCCATCTGTGCCGCCGAGTGGAAGAAACCAGCTGCGTGCGCCCAGCCTGTCCACCCACG

mRNA sequence

AAAAAAGCATAGCCAGAAAACTACAAAAGTTCATTTATTTTTTACCAAAAAAATTCATCAATTTGGATGTGGGTATAGACCAAAATCAGATTTTATTAATTCAGAATGGAAAAACGTTTGTGAGAAAGAAAGAGGAGAGAGAAAAACGGCTCATTCTTCTCAAAATCCTTACTACCTACGCATCTGTTACTGAGGTTGCAGCAGAAAGGGGGAAAAAAAGTGGAGCAAAACAGTTGAAATTTCATTCTGGAAGTGGGAATTTCGGTCATTACCAATTTCTTCCAAAAGGGTCGACATGGAGAGTGATAATGGAGATATGAAGACCAGGATGGAAGACTATGAAGTTATTGAGCAGATTGGGAGGGGGGCATTTGGATCGGCTTTTCTTGTGTATCACAAGACTGAGAAGAAGAAATATGTACTCAAGAAAATTCGCTTGGCAAAGCAAACAGAGAAGTTCAAGCGCACTGCCCACCAAGAGATGAATTTGATTGCAAAACTAAATAACCCATATATTGTGGACTACAAAGATTCATGGGTGGATAAGGGTGATTGTATATGCATTGTAACTGGCTACTGTGAAGGAGGAGACATGTCTGAACTGATCAAAAAGGCCAGAGGAACATATTTTCCGGAGGAAAAACTTTGCAAGTGGTTGGCACAGTTGTTGCTAGCTGTGGACTATCTGCACTCCAATCGAGTACTACATAGAGATCTTAAGTGTTCCAACATTTTCCTTACAAAGGGCAATGATATCCGGCTTGGGGACTTCGGACTTGCAAAACTGCTGAATACAGAAGACCTTGCTTCATCGTGTCTATTTTCTTTTACTGCTTCTAATATGGTATTAGAGCTAGGTAAAGATGAAACCTTAGACATCAACCTTGATGAAACCCAAATTGTTCACAAGACAGTCACCGGGATCAATGTCGCTGCTGCTGCCGCTATGGAAAAATTACTCCAATAACTTCAGACGCCGCCAATGAGCAATTCTGTCTCACCCTCGGAGCCATCTGTGCCGCCGAGTGGAAGAAACCAGCTGCGTGCGCCCAGCCTGTCCACCCACG

Coding sequence (CDS)

ATGGAGAGTGATAATGGAGATATGAAGACCAGGATGGAAGACTATGAAGTTATTGAGCAGATTGGGAGGGGGGCATTTGGATCGGCTTTTCTTGTGTATCACAAGACTGAGAAGAAGAAATATGTACTCAAGAAAATTCGCTTGGCAAAGCAAACAGAGAAGTTCAAGCGCACTGCCCACCAAGAGATGAATTTGATTGCAAAACTAAATAACCCATATATTGTGGACTACAAAGATTCATGGGTGGATAAGGGTGATTGTATATGCATTGTAACTGGCTACTGTGAAGGAGGAGACATGTCTGAACTGATCAAAAAGGCCAGAGGAACATATTTTCCGGAGGAAAAACTTTGCAAGTGGTTGGCACAGTTGTTGCTAGCTGTGGACTATCTGCACTCCAATCGAGTACTACATAGAGATCTTAAGTGTTCCAACATTTTCCTTACAAAGGGCAATGATATCCGGCTTGGGGACTTCGGACTTGCAAAACTGCTGAATACAGAAGACCTTGCTTCATCGTGTCTATTTTCTTTTACTGCTTCTAATATGGTATTAGAGCTAGGTAAAGATGAAACCTTAGACATCAACCTTGATGAAACCCAAATTGTTCACAAGACAGTCACCGGGATCAATGTCGCTGCTGCTGCCGCTATGGAAAAATTACTCCAATAA

Protein sequence

MESDNGDMKTRMEDYEVIEQIGRGAFGSAFLVYHKTEKKKYVLKKIRLAKQTEKFKRTAHQEMNLIAKLNNPYIVDYKDSWVDKGDCICIVTGYCEGGDMSELIKKARGTYFPEEKLCKWLAQLLLAVDYLHSNRVLHRDLKCSNIFLTKGNDIRLGDFGLAKLLNTEDLASSCLFSFTASNMVLELGKDETLDINLDETQIVHKTVTGINVAAAAAMEKLLQ*
Homology
BLAST of CSPI04G21060 vs. ExPASy Swiss-Prot
Match: Q94CU5 (Serine/threonine-protein kinase Nek5 OS=Oryza sativa subsp. japonica OX=39947 GN=NEK5 PE=2 SV=1)

HSP 1 Score: 265.0 bits (676), Expect = 7.7e-70
Identity = 126/166 (75.90%), Postives = 146/166 (87.95%), Query Frame = 0

Query: 8   MKTRMEDYEVIEQIGRGAFGSAFLVYHKTEKKKYVLKKIRLAKQTEKFKRTAHQEMNLIA 67
           M +RM+ YEV+EQIGRGAFG+A LV HKTEKKKYVLKKIRLA+QTE+ +++AHQEM LIA
Sbjct: 1   MDSRMDQYEVMEQIGRGAFGAAILVNHKTEKKKYVLKKIRLARQTERCRKSAHQEMALIA 60

Query: 68  KLNNPYIVDYKDSWVDKGDCICIVTGYCEGGDMSELIKKARGTYFPEEKLCKWLAQLLLA 127
           +L +PYIV++K++WV+KG  +CIVTGYCEGGDM+EL+KKA GTYFPEEKL KW AQL LA
Sbjct: 61  RLQHPYIVEFKEAWVEKGCYVCIVTGYCEGGDMAELMKKANGTYFPEEKLLKWFAQLALA 120

Query: 128 VDYLHSNRVLHRDLKCSNIFLTKGNDIRLGDFGLAKLLNTEDLASS 174
           VDYLHSN VLHRDLKCSNIFLTK  DIRLGDFGLAK L  +DL SS
Sbjct: 121 VDYLHSNFVLHRDLKCSNIFLTKDQDIRLGDFGLAKTLKADDLTSS 166

BLAST of CSPI04G21060 vs. ExPASy Swiss-Prot
Match: Q0WPH8 (Serine/threonine-protein kinase Nek5 OS=Arabidopsis thaliana OX=3702 GN=NEK5 PE=1 SV=1)

HSP 1 Score: 263.8 bits (673), Expect = 1.7e-69
Identity = 120/166 (72.29%), Postives = 148/166 (89.16%), Query Frame = 0

Query: 8   MKTRMEDYEVIEQIGRGAFGSAFLVYHKTEKKKYVLKKIRLAKQTEKFKRTAHQEMNLIA 67
           M++RM+ YE++EQIGRGAFG+A LV+HK E+KKYVLKKIRLA+QTE+ +R+AHQEM+LIA
Sbjct: 1   MESRMDQYELMEQIGRGAFGAAILVHHKAERKKYVLKKIRLARQTERCRRSAHQEMSLIA 60

Query: 68  KLNNPYIVDYKDSWVDKGDCICIVTGYCEGGDMSELIKKARGTYFPEEKLCKWLAQLLLA 127
           ++ +PYIV++K++WV+KG  +CIVTGYCEGGDM+EL+KK+ G YFPEEKLCKW  QLLLA
Sbjct: 61  RVQHPYIVEFKEAWVEKGCYVCIVTGYCEGGDMAELMKKSNGVYFPEEKLCKWFTQLLLA 120

Query: 128 VDYLHSNRVLHRDLKCSNIFLTKGNDIRLGDFGLAKLLNTEDLASS 174
           V+YLHSN VLHRDLKCSNIFLTK  D+RLGDFGLAK L  +DL SS
Sbjct: 121 VEYLHSNYVLHRDLKCSNIFLTKDQDVRLGDFGLAKTLKADDLTSS 166

BLAST of CSPI04G21060 vs. ExPASy Swiss-Prot
Match: Q9LT35 (Serine/threonine-protein kinase Nek6 OS=Arabidopsis thaliana OX=3702 GN=NEK6 PE=3 SV=1)

HSP 1 Score: 261.5 bits (667), Expect = 8.5e-69
Identity = 122/162 (75.31%), Postives = 147/162 (90.74%), Query Frame = 0

Query: 12  MEDYEVIEQIGRGAFGSAFLVYHKTEKKKYVLKKIRLAKQTEKFKRTAHQEMNLIAKLNN 71
           M+DYEV+EQIGRGAFGSAFLV HK+E++KYV+KKIRLAKQTE+ K  A QEM+LI+KL +
Sbjct: 1   MDDYEVVEQIGRGAFGSAFLVIHKSERRKYVVKKIRLAKQTERCKLAAIQEMSLISKLKS 60

Query: 72  PYIVDYKDSWVDKGDCICIVTGYCEGGDMSELIKKARGTYFPEEKLCKWLAQLLLAVDYL 131
           PYIV+YKDSWV+K DC+CIVT YCEGGDM+++IKK+RG +  EEKLC+W+ QLLLA+DYL
Sbjct: 61  PYIVEYKDSWVEK-DCVCIVTSYCEGGDMTQMIKKSRGVFASEEKLCRWMVQLLLAIDYL 120

Query: 132 HSNRVLHRDLKCSNIFLTKGNDIRLGDFGLAKLLNTEDLASS 174
           H+NRVLHRDLKCSNIFLTK N++RLGDFGLAKLL  +DLASS
Sbjct: 121 HNNRVLHRDLKCSNIFLTKENEVRLGDFGLAKLLGKDDLASS 161

BLAST of CSPI04G21060 vs. ExPASy Swiss-Prot
Match: Q60DG4 (Serine/threonine-protein kinase Nek4 OS=Oryza sativa subsp. japonica OX=39947 GN=NEK4 PE=2 SV=1)

HSP 1 Score: 260.0 bits (663), Expect = 2.5e-68
Identity = 121/166 (72.89%), Postives = 145/166 (87.35%), Query Frame = 0

Query: 8   MKTRMEDYEVIEQIGRGAFGSAFLVYHKTEKKKYVLKKIRLAKQTEKFKRTAHQEMNLIA 67
           M++RM+ YE++EQ+GRGAFG+A LV HK E+KKYVLKKIRLA+QTE+ +++AHQEM LIA
Sbjct: 1   MESRMDQYEIMEQVGRGAFGAAILVNHKIERKKYVLKKIRLARQTERCRKSAHQEMALIA 60

Query: 68  KLNNPYIVDYKDSWVDKGDCICIVTGYCEGGDMSELIKKARGTYFPEEKLCKWLAQLLLA 127
           +L +PYIV++K++WV+KG  +CIVTGYCEGGDM EL+KK  GTYFPEEKL KW AQL+LA
Sbjct: 61  RLQHPYIVEFKEAWVEKGCYVCIVTGYCEGGDMDELMKKLNGTYFPEEKLLKWFAQLVLA 120

Query: 128 VDYLHSNRVLHRDLKCSNIFLTKGNDIRLGDFGLAKLLNTEDLASS 174
           VDYLHSN VLHRDLKCSNIFLTK  DIRLGDFGLAK L  +DL SS
Sbjct: 121 VDYLHSNYVLHRDLKCSNIFLTKDQDIRLGDFGLAKTLKEDDLTSS 166

BLAST of CSPI04G21060 vs. ExPASy Swiss-Prot
Match: A2ZMH2 (Serine/threonine-protein kinase Nek2 OS=Oryza sativa subsp. indica OX=39946 GN=NEK2 PE=3 SV=1)

HSP 1 Score: 254.2 bits (648), Expect = 1.4e-66
Identity = 122/179 (68.16%), Postives = 148/179 (82.68%), Query Frame = 0

Query: 12  MEDYEVIEQIGRGAFGSAFLVYHKTEKKKYVLKKIRLAKQTEKFKRTAHQEMNLIAKLNN 71
           M+ YEV+EQIG+GAFGSA LV HK EKKKYVLKKIRLA+QT++ +R+AHQEM LIA + N
Sbjct: 1   MDQYEVLEQIGKGAFGSALLVRHKLEKKKYVLKKIRLARQTDRTRRSAHQEMQLIATVRN 60

Query: 72  PYIVDYKDSWVDKGDCICIVTGYCEGGDMSELIKKARGTYFPEEKLCKWLAQLLLAVDYL 131
           P+IV+YKDSWV+KG  +CIV GYCEGGDM+E IK+A GTYF EEKLCKWL QLL+A+DYL
Sbjct: 61  PFIVEYKDSWVEKGCYVCIVIGYCEGGDMAEAIKRANGTYFSEEKLCKWLVQLLMALDYL 120

Query: 132 HSNRVLHRDLKCSNIFLTKGNDIRLGDFGLAKLLNTEDLASSCLFSFTASNMVLELGKD 191
           H+N +LHRD+KCSNIF+ +   IRLGDFGLAK+L ++DLASS +   T S M  EL  D
Sbjct: 121 HANHILHRDVKCSNIFIARDQSIRLGDFGLAKILTSDDLASSVV--GTPSYMCPELLAD 177

BLAST of CSPI04G21060 vs. ExPASy TrEMBL
Match: A0A1S3B618 (serine/threonine-protein kinase Nek6 isoform X2 OS=Cucumis melo OX=3656 GN=LOC103486166 PE=4 SV=1)

HSP 1 Score: 355.9 bits (912), Expect = 1.2e-94
Identity = 173/173 (100.00%), Postives = 173/173 (100.00%), Query Frame = 0

Query: 1   MESDNGDMKTRMEDYEVIEQIGRGAFGSAFLVYHKTEKKKYVLKKIRLAKQTEKFKRTAH 60
           MESDNGDMKTRMEDYEVIEQIGRGAFGSAFLVYHKTEKKKYVLKKIRLAKQTEKFKRTAH
Sbjct: 1   MESDNGDMKTRMEDYEVIEQIGRGAFGSAFLVYHKTEKKKYVLKKIRLAKQTEKFKRTAH 60

Query: 61  QEMNLIAKLNNPYIVDYKDSWVDKGDCICIVTGYCEGGDMSELIKKARGTYFPEEKLCKW 120
           QEMNLIAKLNNPYIVDYKDSWVDKGDCICIVTGYCEGGDMSELIKKARGTYFPEEKLCKW
Sbjct: 61  QEMNLIAKLNNPYIVDYKDSWVDKGDCICIVTGYCEGGDMSELIKKARGTYFPEEKLCKW 120

Query: 121 LAQLLLAVDYLHSNRVLHRDLKCSNIFLTKGNDIRLGDFGLAKLLNTEDLASS 174
           LAQLLLAVDYLHSNRVLHRDLKCSNIFLTKGNDIRLGDFGLAKLLNTEDLASS
Sbjct: 121 LAQLLLAVDYLHSNRVLHRDLKCSNIFLTKGNDIRLGDFGLAKLLNTEDLASS 173

BLAST of CSPI04G21060 vs. ExPASy TrEMBL
Match: A0A5D3E248 (Serine/threonine-protein kinase Nek6 isoform X1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold208G001040 PE=4 SV=1)

HSP 1 Score: 355.9 bits (912), Expect = 1.2e-94
Identity = 173/173 (100.00%), Postives = 173/173 (100.00%), Query Frame = 0

Query: 1   MESDNGDMKTRMEDYEVIEQIGRGAFGSAFLVYHKTEKKKYVLKKIRLAKQTEKFKRTAH 60
           MESDNGDMKTRMEDYEVIEQIGRGAFGSAFLVYHKTEKKKYVLKKIRLAKQTEKFKRTAH
Sbjct: 111 MESDNGDMKTRMEDYEVIEQIGRGAFGSAFLVYHKTEKKKYVLKKIRLAKQTEKFKRTAH 170

Query: 61  QEMNLIAKLNNPYIVDYKDSWVDKGDCICIVTGYCEGGDMSELIKKARGTYFPEEKLCKW 120
           QEMNLIAKLNNPYIVDYKDSWVDKGDCICIVTGYCEGGDMSELIKKARGTYFPEEKLCKW
Sbjct: 171 QEMNLIAKLNNPYIVDYKDSWVDKGDCICIVTGYCEGGDMSELIKKARGTYFPEEKLCKW 230

Query: 121 LAQLLLAVDYLHSNRVLHRDLKCSNIFLTKGNDIRLGDFGLAKLLNTEDLASS 174
           LAQLLLAVDYLHSNRVLHRDLKCSNIFLTKGNDIRLGDFGLAKLLNTEDLASS
Sbjct: 231 LAQLLLAVDYLHSNRVLHRDLKCSNIFLTKGNDIRLGDFGLAKLLNTEDLASS 283

BLAST of CSPI04G21060 vs. ExPASy TrEMBL
Match: A0A5A7T2B9 (Serine/threonine-protein kinase Nek6 isoform X1 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold403G00350 PE=4 SV=1)

HSP 1 Score: 355.9 bits (912), Expect = 1.2e-94
Identity = 173/173 (100.00%), Postives = 173/173 (100.00%), Query Frame = 0

Query: 1   MESDNGDMKTRMEDYEVIEQIGRGAFGSAFLVYHKTEKKKYVLKKIRLAKQTEKFKRTAH 60
           MESDNGDMKTRMEDYEVIEQIGRGAFGSAFLVYHKTEKKKYVLKKIRLAKQTEKFKRTAH
Sbjct: 1   MESDNGDMKTRMEDYEVIEQIGRGAFGSAFLVYHKTEKKKYVLKKIRLAKQTEKFKRTAH 60

Query: 61  QEMNLIAKLNNPYIVDYKDSWVDKGDCICIVTGYCEGGDMSELIKKARGTYFPEEKLCKW 120
           QEMNLIAKLNNPYIVDYKDSWVDKGDCICIVTGYCEGGDMSELIKKARGTYFPEEKLCKW
Sbjct: 61  QEMNLIAKLNNPYIVDYKDSWVDKGDCICIVTGYCEGGDMSELIKKARGTYFPEEKLCKW 120

Query: 121 LAQLLLAVDYLHSNRVLHRDLKCSNIFLTKGNDIRLGDFGLAKLLNTEDLASS 174
           LAQLLLAVDYLHSNRVLHRDLKCSNIFLTKGNDIRLGDFGLAKLLNTEDLASS
Sbjct: 121 LAQLLLAVDYLHSNRVLHRDLKCSNIFLTKGNDIRLGDFGLAKLLNTEDLASS 173

BLAST of CSPI04G21060 vs. ExPASy TrEMBL
Match: A0A0A0L1F2 (Protein kinase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_4G572290 PE=3 SV=1)

HSP 1 Score: 355.9 bits (912), Expect = 1.2e-94
Identity = 173/173 (100.00%), Postives = 173/173 (100.00%), Query Frame = 0

Query: 1   MESDNGDMKTRMEDYEVIEQIGRGAFGSAFLVYHKTEKKKYVLKKIRLAKQTEKFKRTAH 60
           MESDNGDMKTRMEDYEVIEQIGRGAFGSAFLVYHKTEKKKYVLKKIRLAKQTEKFKRTAH
Sbjct: 1   MESDNGDMKTRMEDYEVIEQIGRGAFGSAFLVYHKTEKKKYVLKKIRLAKQTEKFKRTAH 60

Query: 61  QEMNLIAKLNNPYIVDYKDSWVDKGDCICIVTGYCEGGDMSELIKKARGTYFPEEKLCKW 120
           QEMNLIAKLNNPYIVDYKDSWVDKGDCICIVTGYCEGGDMSELIKKARGTYFPEEKLCKW
Sbjct: 61  QEMNLIAKLNNPYIVDYKDSWVDKGDCICIVTGYCEGGDMSELIKKARGTYFPEEKLCKW 120

Query: 121 LAQLLLAVDYLHSNRVLHRDLKCSNIFLTKGNDIRLGDFGLAKLLNTEDLASS 174
           LAQLLLAVDYLHSNRVLHRDLKCSNIFLTKGNDIRLGDFGLAKLLNTEDLASS
Sbjct: 121 LAQLLLAVDYLHSNRVLHRDLKCSNIFLTKGNDIRLGDFGLAKLLNTEDLASS 173

BLAST of CSPI04G21060 vs. ExPASy TrEMBL
Match: A0A1S3B598 (serine/threonine-protein kinase Nek6 isoform X1 OS=Cucumis melo OX=3656 GN=LOC103486166 PE=4 SV=1)

HSP 1 Score: 355.9 bits (912), Expect = 1.2e-94
Identity = 173/173 (100.00%), Postives = 173/173 (100.00%), Query Frame = 0

Query: 1   MESDNGDMKTRMEDYEVIEQIGRGAFGSAFLVYHKTEKKKYVLKKIRLAKQTEKFKRTAH 60
           MESDNGDMKTRMEDYEVIEQIGRGAFGSAFLVYHKTEKKKYVLKKIRLAKQTEKFKRTAH
Sbjct: 10  MESDNGDMKTRMEDYEVIEQIGRGAFGSAFLVYHKTEKKKYVLKKIRLAKQTEKFKRTAH 69

Query: 61  QEMNLIAKLNNPYIVDYKDSWVDKGDCICIVTGYCEGGDMSELIKKARGTYFPEEKLCKW 120
           QEMNLIAKLNNPYIVDYKDSWVDKGDCICIVTGYCEGGDMSELIKKARGTYFPEEKLCKW
Sbjct: 70  QEMNLIAKLNNPYIVDYKDSWVDKGDCICIVTGYCEGGDMSELIKKARGTYFPEEKLCKW 129

Query: 121 LAQLLLAVDYLHSNRVLHRDLKCSNIFLTKGNDIRLGDFGLAKLLNTEDLASS 174
           LAQLLLAVDYLHSNRVLHRDLKCSNIFLTKGNDIRLGDFGLAKLLNTEDLASS
Sbjct: 130 LAQLLLAVDYLHSNRVLHRDLKCSNIFLTKGNDIRLGDFGLAKLLNTEDLASS 182

BLAST of CSPI04G21060 vs. NCBI nr
Match: XP_011653939.1 (serine/threonine-protein kinase Nek6 [Cucumis sativus] >XP_011653940.1 serine/threonine-protein kinase Nek6 [Cucumis sativus] >XP_011653941.1 serine/threonine-protein kinase Nek6 [Cucumis sativus] >KGN55326.2 hypothetical protein Csa_012359 [Cucumis sativus])

HSP 1 Score: 355.9 bits (912), Expect = 2.5e-94
Identity = 173/173 (100.00%), Postives = 173/173 (100.00%), Query Frame = 0

Query: 1   MESDNGDMKTRMEDYEVIEQIGRGAFGSAFLVYHKTEKKKYVLKKIRLAKQTEKFKRTAH 60
           MESDNGDMKTRMEDYEVIEQIGRGAFGSAFLVYHKTEKKKYVLKKIRLAKQTEKFKRTAH
Sbjct: 1   MESDNGDMKTRMEDYEVIEQIGRGAFGSAFLVYHKTEKKKYVLKKIRLAKQTEKFKRTAH 60

Query: 61  QEMNLIAKLNNPYIVDYKDSWVDKGDCICIVTGYCEGGDMSELIKKARGTYFPEEKLCKW 120
           QEMNLIAKLNNPYIVDYKDSWVDKGDCICIVTGYCEGGDMSELIKKARGTYFPEEKLCKW
Sbjct: 61  QEMNLIAKLNNPYIVDYKDSWVDKGDCICIVTGYCEGGDMSELIKKARGTYFPEEKLCKW 120

Query: 121 LAQLLLAVDYLHSNRVLHRDLKCSNIFLTKGNDIRLGDFGLAKLLNTEDLASS 174
           LAQLLLAVDYLHSNRVLHRDLKCSNIFLTKGNDIRLGDFGLAKLLNTEDLASS
Sbjct: 121 LAQLLLAVDYLHSNRVLHRDLKCSNIFLTKGNDIRLGDFGLAKLLNTEDLASS 173

BLAST of CSPI04G21060 vs. NCBI nr
Match: XP_008442255.1 (PREDICTED: serine/threonine-protein kinase Nek6 isoform X1 [Cucumis melo])

HSP 1 Score: 355.9 bits (912), Expect = 2.5e-94
Identity = 173/173 (100.00%), Postives = 173/173 (100.00%), Query Frame = 0

Query: 1   MESDNGDMKTRMEDYEVIEQIGRGAFGSAFLVYHKTEKKKYVLKKIRLAKQTEKFKRTAH 60
           MESDNGDMKTRMEDYEVIEQIGRGAFGSAFLVYHKTEKKKYVLKKIRLAKQTEKFKRTAH
Sbjct: 10  MESDNGDMKTRMEDYEVIEQIGRGAFGSAFLVYHKTEKKKYVLKKIRLAKQTEKFKRTAH 69

Query: 61  QEMNLIAKLNNPYIVDYKDSWVDKGDCICIVTGYCEGGDMSELIKKARGTYFPEEKLCKW 120
           QEMNLIAKLNNPYIVDYKDSWVDKGDCICIVTGYCEGGDMSELIKKARGTYFPEEKLCKW
Sbjct: 70  QEMNLIAKLNNPYIVDYKDSWVDKGDCICIVTGYCEGGDMSELIKKARGTYFPEEKLCKW 129

Query: 121 LAQLLLAVDYLHSNRVLHRDLKCSNIFLTKGNDIRLGDFGLAKLLNTEDLASS 174
           LAQLLLAVDYLHSNRVLHRDLKCSNIFLTKGNDIRLGDFGLAKLLNTEDLASS
Sbjct: 130 LAQLLLAVDYLHSNRVLHRDLKCSNIFLTKGNDIRLGDFGLAKLLNTEDLASS 182

BLAST of CSPI04G21060 vs. NCBI nr
Match: XP_008442256.1 (PREDICTED: serine/threonine-protein kinase Nek6 isoform X2 [Cucumis melo] >XP_008442257.1 PREDICTED: serine/threonine-protein kinase Nek6 isoform X2 [Cucumis melo] >XP_008442258.1 PREDICTED: serine/threonine-protein kinase Nek6 isoform X2 [Cucumis melo] >XP_008442259.1 PREDICTED: serine/threonine-protein kinase Nek6 isoform X2 [Cucumis melo] >KAA0035761.1 serine/threonine-protein kinase Nek6 isoform X1 [Cucumis melo var. makuwa])

HSP 1 Score: 355.9 bits (912), Expect = 2.5e-94
Identity = 173/173 (100.00%), Postives = 173/173 (100.00%), Query Frame = 0

Query: 1   MESDNGDMKTRMEDYEVIEQIGRGAFGSAFLVYHKTEKKKYVLKKIRLAKQTEKFKRTAH 60
           MESDNGDMKTRMEDYEVIEQIGRGAFGSAFLVYHKTEKKKYVLKKIRLAKQTEKFKRTAH
Sbjct: 1   MESDNGDMKTRMEDYEVIEQIGRGAFGSAFLVYHKTEKKKYVLKKIRLAKQTEKFKRTAH 60

Query: 61  QEMNLIAKLNNPYIVDYKDSWVDKGDCICIVTGYCEGGDMSELIKKARGTYFPEEKLCKW 120
           QEMNLIAKLNNPYIVDYKDSWVDKGDCICIVTGYCEGGDMSELIKKARGTYFPEEKLCKW
Sbjct: 61  QEMNLIAKLNNPYIVDYKDSWVDKGDCICIVTGYCEGGDMSELIKKARGTYFPEEKLCKW 120

Query: 121 LAQLLLAVDYLHSNRVLHRDLKCSNIFLTKGNDIRLGDFGLAKLLNTEDLASS 174
           LAQLLLAVDYLHSNRVLHRDLKCSNIFLTKGNDIRLGDFGLAKLLNTEDLASS
Sbjct: 121 LAQLLLAVDYLHSNRVLHRDLKCSNIFLTKGNDIRLGDFGLAKLLNTEDLASS 173

BLAST of CSPI04G21060 vs. NCBI nr
Match: XP_038881602.1 (serine/threonine-protein kinase Nek6 [Benincasa hispida] >XP_038881603.1 serine/threonine-protein kinase Nek6 [Benincasa hispida] >XP_038881604.1 serine/threonine-protein kinase Nek6 [Benincasa hispida] >XP_038881605.1 serine/threonine-protein kinase Nek6 [Benincasa hispida])

HSP 1 Score: 355.9 bits (912), Expect = 2.5e-94
Identity = 173/173 (100.00%), Postives = 173/173 (100.00%), Query Frame = 0

Query: 1   MESDNGDMKTRMEDYEVIEQIGRGAFGSAFLVYHKTEKKKYVLKKIRLAKQTEKFKRTAH 60
           MESDNGDMKTRMEDYEVIEQIGRGAFGSAFLVYHKTEKKKYVLKKIRLAKQTEKFKRTAH
Sbjct: 1   MESDNGDMKTRMEDYEVIEQIGRGAFGSAFLVYHKTEKKKYVLKKIRLAKQTEKFKRTAH 60

Query: 61  QEMNLIAKLNNPYIVDYKDSWVDKGDCICIVTGYCEGGDMSELIKKARGTYFPEEKLCKW 120
           QEMNLIAKLNNPYIVDYKDSWVDKGDCICIVTGYCEGGDMSELIKKARGTYFPEEKLCKW
Sbjct: 61  QEMNLIAKLNNPYIVDYKDSWVDKGDCICIVTGYCEGGDMSELIKKARGTYFPEEKLCKW 120

Query: 121 LAQLLLAVDYLHSNRVLHRDLKCSNIFLTKGNDIRLGDFGLAKLLNTEDLASS 174
           LAQLLLAVDYLHSNRVLHRDLKCSNIFLTKGNDIRLGDFGLAKLLNTEDLASS
Sbjct: 121 LAQLLLAVDYLHSNRVLHRDLKCSNIFLTKGNDIRLGDFGLAKLLNTEDLASS 173

BLAST of CSPI04G21060 vs. NCBI nr
Match: TYK29858.1 (serine/threonine-protein kinase Nek6 isoform X1 [Cucumis melo var. makuwa])

HSP 1 Score: 355.9 bits (912), Expect = 2.5e-94
Identity = 173/173 (100.00%), Postives = 173/173 (100.00%), Query Frame = 0

Query: 1   MESDNGDMKTRMEDYEVIEQIGRGAFGSAFLVYHKTEKKKYVLKKIRLAKQTEKFKRTAH 60
           MESDNGDMKTRMEDYEVIEQIGRGAFGSAFLVYHKTEKKKYVLKKIRLAKQTEKFKRTAH
Sbjct: 111 MESDNGDMKTRMEDYEVIEQIGRGAFGSAFLVYHKTEKKKYVLKKIRLAKQTEKFKRTAH 170

Query: 61  QEMNLIAKLNNPYIVDYKDSWVDKGDCICIVTGYCEGGDMSELIKKARGTYFPEEKLCKW 120
           QEMNLIAKLNNPYIVDYKDSWVDKGDCICIVTGYCEGGDMSELIKKARGTYFPEEKLCKW
Sbjct: 171 QEMNLIAKLNNPYIVDYKDSWVDKGDCICIVTGYCEGGDMSELIKKARGTYFPEEKLCKW 230

Query: 121 LAQLLLAVDYLHSNRVLHRDLKCSNIFLTKGNDIRLGDFGLAKLLNTEDLASS 174
           LAQLLLAVDYLHSNRVLHRDLKCSNIFLTKGNDIRLGDFGLAKLLNTEDLASS
Sbjct: 231 LAQLLLAVDYLHSNRVLHRDLKCSNIFLTKGNDIRLGDFGLAKLLNTEDLASS 283

BLAST of CSPI04G21060 vs. TAIR 10
Match: AT3G44200.1 (NIMA (never in mitosis, gene A)-related 6 )

HSP 1 Score: 263.8 bits (673), Expect = 1.2e-70
Identity = 120/166 (72.29%), Postives = 148/166 (89.16%), Query Frame = 0

Query: 8   MKTRMEDYEVIEQIGRGAFGSAFLVYHKTEKKKYVLKKIRLAKQTEKFKRTAHQEMNLIA 67
           M++RM+ YE++EQIGRGAFG+A LV+HK E+KKYVLKKIRLA+QTE+ +R+AHQEM+LIA
Sbjct: 1   MESRMDQYELMEQIGRGAFGAAILVHHKAERKKYVLKKIRLARQTERCRRSAHQEMSLIA 60

Query: 68  KLNNPYIVDYKDSWVDKGDCICIVTGYCEGGDMSELIKKARGTYFPEEKLCKWLAQLLLA 127
           ++ +PYIV++K++WV+KG  +CIVTGYCEGGDM+EL+KK+ G YFPEEKLCKW  QLLLA
Sbjct: 61  RVQHPYIVEFKEAWVEKGCYVCIVTGYCEGGDMAELMKKSNGVYFPEEKLCKWFTQLLLA 120

Query: 128 VDYLHSNRVLHRDLKCSNIFLTKGNDIRLGDFGLAKLLNTEDLASS 174
           V+YLHSN VLHRDLKCSNIFLTK  D+RLGDFGLAK L  +DL SS
Sbjct: 121 VEYLHSNYVLHRDLKCSNIFLTKDQDVRLGDFGLAKTLKADDLTSS 166

BLAST of CSPI04G21060 vs. TAIR 10
Match: AT3G20860.1 (NIMA-related kinase 5 )

HSP 1 Score: 262.7 bits (670), Expect = 2.7e-70
Identity = 123/173 (71.10%), Postives = 152/173 (87.86%), Query Frame = 0

Query: 1   MESDNGDMKTRMEDYEVIEQIGRGAFGSAFLVYHKTEKKKYVLKKIRLAKQTEKFKRTAH 60
           M +   +  ++M+DYEV+EQIGRGAFGSAFLV HK+E++KYV+KKIRLAKQTE+ K  A 
Sbjct: 1   MANKISETASKMDDYEVVEQIGRGAFGSAFLVIHKSERRKYVVKKIRLAKQTERCKLAAI 60

Query: 61  QEMNLIAKLNNPYIVDYKDSWVDKGDCICIVTGYCEGGDMSELIKKARGTYFPEEKLCKW 120
           QEM+LI+KL +PYIV+YKDSWV+K DC+CIVT YCEGGDM+++IKK+RG +  EEKLC+W
Sbjct: 61  QEMSLISKLKSPYIVEYKDSWVEK-DCVCIVTSYCEGGDMTQMIKKSRGVFASEEKLCRW 120

Query: 121 LAQLLLAVDYLHSNRVLHRDLKCSNIFLTKGNDIRLGDFGLAKLLNTEDLASS 174
           + QLLLA+DYLH+NRVLHRDLKCSNIFLTK N++RLGDFGLAKLL  +DLASS
Sbjct: 121 MVQLLLAIDYLHNNRVLHRDLKCSNIFLTKENEVRLGDFGLAKLLGKDDLASS 172

BLAST of CSPI04G21060 vs. TAIR 10
Match: AT3G04810.1 (NIMA-related kinase 2 )

HSP 1 Score: 250.0 bits (637), Expect = 1.8e-66
Identity = 120/179 (67.04%), Postives = 149/179 (83.24%), Query Frame = 0

Query: 12  MEDYEVIEQIGRGAFGSAFLVYHKTEKKKYVLKKIRLAKQTEKFKRTAHQEMNLIAKLNN 71
           ME+YEV+EQIG+G+FGSA LV HK EKK YVLKKIRLA+QT + +R+AHQEM LI+K++N
Sbjct: 1   MENYEVLEQIGKGSFGSALLVRHKHEKKLYVLKKIRLARQTGRTRRSAHQEMELISKIHN 60

Query: 72  PYIVDYKDSWVDKGDCICIVTGYCEGGDMSELIKKARGTYFPEEKLCKWLAQLLLAVDYL 131
           P+IV+YKDSWV+KG  +CI+ GYC+GGDM+E IKK  G +F EEKLCKWL Q+LLA++YL
Sbjct: 61  PFIVEYKDSWVEKGCYVCIIIGYCKGGDMAEAIKKTNGVHFTEEKLCKWLVQILLALEYL 120

Query: 132 HSNRVLHRDLKCSNIFLTKGNDIRLGDFGLAKLLNTEDLASSCLFSFTASNMVLELGKD 191
           H+N +LHRD+KCSNIFLTK  DIRLGDFGLAK+L ++DLASS +   T S M  EL  D
Sbjct: 121 HANHILHRDVKCSNIFLTKDQDIRLGDFGLAKVLTSDDLASSVV--GTPSYMCPELLAD 177

BLAST of CSPI04G21060 vs. TAIR 10
Match: AT3G04810.2 (NIMA-related kinase 2 )

HSP 1 Score: 250.0 bits (637), Expect = 1.8e-66
Identity = 120/179 (67.04%), Postives = 149/179 (83.24%), Query Frame = 0

Query: 12  MEDYEVIEQIGRGAFGSAFLVYHKTEKKKYVLKKIRLAKQTEKFKRTAHQEMNLIAKLNN 71
           ME+YEV+EQIG+G+FGSA LV HK EKK YVLKKIRLA+QT + +R+AHQEM LI+K++N
Sbjct: 1   MENYEVLEQIGKGSFGSALLVRHKHEKKLYVLKKIRLARQTGRTRRSAHQEMELISKIHN 60

Query: 72  PYIVDYKDSWVDKGDCICIVTGYCEGGDMSELIKKARGTYFPEEKLCKWLAQLLLAVDYL 131
           P+IV+YKDSWV+KG  +CI+ GYC+GGDM+E IKK  G +F EEKLCKWL Q+LLA++YL
Sbjct: 61  PFIVEYKDSWVEKGCYVCIIIGYCKGGDMAEAIKKTNGVHFTEEKLCKWLVQILLALEYL 120

Query: 132 HSNRVLHRDLKCSNIFLTKGNDIRLGDFGLAKLLNTEDLASSCLFSFTASNMVLELGKD 191
           H+N +LHRD+KCSNIFLTK  DIRLGDFGLAK+L ++DLASS +   T S M  EL  D
Sbjct: 121 HANHILHRDVKCSNIFLTKDQDIRLGDFGLAKVLTSDDLASSVV--GTPSYMCPELLAD 177

BLAST of CSPI04G21060 vs. TAIR 10
Match: AT3G63280.1 (NIMA-related kinase 4 )

HSP 1 Score: 248.8 bits (634), Expect = 4.0e-66
Identity = 118/179 (65.92%), Postives = 149/179 (83.24%), Query Frame = 0

Query: 12  MEDYEVIEQIGRGAFGSAFLVYHKTEKKKYVLKKIRLAKQTEKFKRTAHQEMNLIAKLNN 71
           ME YEV+EQIG+G+FGSA LV HK E+KKYVLKKIRLA+Q+++ +R+AHQEM LI+ + N
Sbjct: 1   MERYEVLEQIGKGSFGSALLVRHKQERKKYVLKKIRLARQSDRARRSAHQEMELISTVRN 60

Query: 72  PYIVDYKDSWVDKGDCICIVTGYCEGGDMSELIKKARGTYFPEEKLCKWLAQLLLAVDYL 131
           P++V+YKDSWV+KG  +CIV GYC+GGDM++ IK+A G +FPEEKLC+WL QLL+A+DYL
Sbjct: 61  PFVVEYKDSWVEKGCYVCIVIGYCQGGDMTDTIKRACGVHFPEEKLCQWLVQLLMALDYL 120

Query: 132 HSNRVLHRDLKCSNIFLTKGNDIRLGDFGLAKLLNTEDLASSCLFSFTASNMVLELGKD 191
           HSN +LHRD+KCSNIFLTK  DIRLGDFGLAK+L ++DL SS +   T S M  EL  D
Sbjct: 121 HSNHILHRDVKCSNIFLTKEQDIRLGDFGLAKILTSDDLTSSVV--GTPSYMCPELLAD 177

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q94CU57.7e-7075.90Serine/threonine-protein kinase Nek5 OS=Oryza sativa subsp. japonica OX=39947 GN... [more]
Q0WPH81.7e-6972.29Serine/threonine-protein kinase Nek5 OS=Arabidopsis thaliana OX=3702 GN=NEK5 PE=... [more]
Q9LT358.5e-6975.31Serine/threonine-protein kinase Nek6 OS=Arabidopsis thaliana OX=3702 GN=NEK6 PE=... [more]
Q60DG42.5e-6872.89Serine/threonine-protein kinase Nek4 OS=Oryza sativa subsp. japonica OX=39947 GN... [more]
A2ZMH21.4e-6668.16Serine/threonine-protein kinase Nek2 OS=Oryza sativa subsp. indica OX=39946 GN=N... [more]
Match NameE-valueIdentityDescription
A0A1S3B6181.2e-94100.00serine/threonine-protein kinase Nek6 isoform X2 OS=Cucumis melo OX=3656 GN=LOC10... [more]
A0A5D3E2481.2e-94100.00Serine/threonine-protein kinase Nek6 isoform X1 OS=Cucumis melo var. makuwa OX=1... [more]
A0A5A7T2B91.2e-94100.00Serine/threonine-protein kinase Nek6 isoform X1 OS=Cucumis melo var. makuwa OX=1... [more]
A0A0A0L1F21.2e-94100.00Protein kinase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_4G572... [more]
A0A1S3B5981.2e-94100.00serine/threonine-protein kinase Nek6 isoform X1 OS=Cucumis melo OX=3656 GN=LOC10... [more]
Match NameE-valueIdentityDescription
XP_011653939.12.5e-94100.00serine/threonine-protein kinase Nek6 [Cucumis sativus] >XP_011653940.1 serine/th... [more]
XP_008442255.12.5e-94100.00PREDICTED: serine/threonine-protein kinase Nek6 isoform X1 [Cucumis melo][more]
XP_008442256.12.5e-94100.00PREDICTED: serine/threonine-protein kinase Nek6 isoform X2 [Cucumis melo] >XP_00... [more]
XP_038881602.12.5e-94100.00serine/threonine-protein kinase Nek6 [Benincasa hispida] >XP_038881603.1 serine/... [more]
TYK29858.12.5e-94100.00serine/threonine-protein kinase Nek6 isoform X1 [Cucumis melo var. makuwa][more]
Match NameE-valueIdentityDescription
AT3G44200.11.2e-7072.29NIMA (never in mitosis, gene A)-related 6 [more]
AT3G20860.12.7e-7071.10NIMA-related kinase 5 [more]
AT3G04810.11.8e-6667.04NIMA-related kinase 2 [more]
AT3G04810.21.8e-6667.04NIMA-related kinase 2 [more]
AT3G63280.14.0e-6665.92NIMA-related kinase 4 [more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (PI 183967) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000719Protein kinase domainSMARTSM00220serkin_6coord: 15..222
e-value: 6.0E-26
score: 102.2
IPR000719Protein kinase domainPFAMPF00069Pkinasecoord: 15..173
e-value: 4.1E-41
score: 141.1
IPR000719Protein kinase domainPROSITEPS50011PROTEIN_KINASE_DOMcoord: 15..223
score: 30.11038
NoneNo IPR availableGENE3D1.10.510.10Transferase(Phosphotransferase) domain 1coord: 91..195
e-value: 7.3E-24
score: 86.1
NoneNo IPR availableGENE3D3.30.200.20Phosphorylase Kinase; domain 1coord: 2..90
e-value: 1.3E-23
score: 85.1
NoneNo IPR availablePANTHERPTHR43671:SF70SERINE/THREONINE-PROTEIN KINASE NEK7coord: 8..187
NoneNo IPR availablePANTHERPTHR43671SERINE/THREONINE-PROTEIN KINASE NEKcoord: 8..187
IPR017441Protein kinase, ATP binding sitePROSITEPS00107PROTEIN_KINASE_ATPcoord: 21..44
IPR008271Serine/threonine-protein kinase, active sitePROSITEPS00108PROTEIN_KINASE_STcoord: 136..148
IPR011009Protein kinase-like domain superfamilySUPERFAMILY56112Protein kinase-like (PK-like)coord: 11..171

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CSPI04G21060.1CSPI04G21060.1mRNA
CSPI04G21060.2CSPI04G21060.2mRNA
CSPI04G21060.3CSPI04G21060.3mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006468 protein phosphorylation
molecular_function GO:0005524 ATP binding
molecular_function GO:0004674 protein serine/threonine kinase activity
molecular_function GO:0004672 protein kinase activity