CSPI04G20130 (gene) Cucumber (PI 183967) v1

Overview
NameCSPI04G20130
Typegene
OrganismCucumis sativus var. hardwickii cv. PI 183967 (Cucumber (PI 183967) v1)
DescriptionE3 ubiquitin-protein ligase CHFR isoform X1
LocationChr4: 18188790 .. 18192468 (-)
RNA-Seq ExpressionCSPI04G20130
SyntenyCSPI04G20130
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRpolypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
AAAATTGTTTCATTTCAAAATTCTCAATCTTCTCCTTCTCCTACTGGCGGTATGGCGGAAATTGGAGAGTGCTCTGCTTCAAATTCATCCATGGAAAACTGGGCGAAGCTAAGTATGTTTCATCTTCCCATGTCTCTCCTCCCTGAGTTCTTCTCAGTCTGTCTCTTGTTTGACTGATCCGATGGCTAATCCGTTTTGCATTAGTGGATTCTCTGTTTCATTTGCTTTTGCTCAGCATTTGAGAATTGGGCATTTTTTTTATAATTTTTTTTGCATTCTTTGCAAACAAAAATTTGTTACTATCCTGAAAAAGTTTTCTTCAGTTTGAAGTCTGGGTTGAAATTACTGGGGAAGATAATGTCATCGAGGAAATAATTTTGGTGGTACGGGAGTTGAACTAGGTTTGACAATGATGGAAGTTCAATGTTTCATTTGTATTTGATTTCATGAACTGTTAGCAGTATGATTTACATAGTAGAAGGTAATTGTCTCACTAATGTTTCTTTCGGTGTTTTAAATTCATTTTAGGGAAATAGGCAGACAGTATAGAAATTAAAGAATTTCTATTTTGATGAAAGATATACAAGAGGAAGAAGAACGATTAGGAATCGTACTCTCCCACTTAACTTAAATGATCACAGAAAGCATGGGAAAATTTTACAAGAACATCCATGCAAAGAACACATCTAGTCATCGAAAACGATCAAATGAATCAAAGTTTATTTAACCTCTCTGAATTTATAGCTCAAAATCTATATATCTTCCATTCCAATTTTCTCAAAATTTTTTTACTTTAACATGAAAAAGTGACGGGATAAAGCTTTCACGTTCTTAGAAGGATTCCCATACCCTTGAGGAAATGATTACATTAAAGTTTGTCTCAATTTCCATGAACAAGGTAGTTGTAATACGATCATGGATTTGCATGAATTAAAATTGATGGTGGATTGAAAATTATTCTTGAACATATTTGTCATTAAAATATATGTATTTATATTAGAAAAAAAAACAAGAAACACAAAATTTCACAAATGATTGAAAATTTATAAAAGAGTACAAGGATTCAAAGACTACAATGTGTAGACGTGGGAAAACAAAAGTTGCAAGTGGAAGAAAATTGCAAAATAGAAAGAGGGAAAGCTATAACTTGACCATAAAAAAAGCCTCTCAATCGTTTATTGTCATTTACAGATCTTAATATCAAGATTACACCTTTGGGATGATTTCCTTTCAAGAAGCCTTTCTTTCAATTTCCTATGTTGGAATTTTGGTTGCTACCACTTATCTTGAATAGCTATTTTTCTTCCTCTAATACCAAAAAATGATGTAATCTAGCATCTAAGTGAATAATCTCGAAGAATACTCAACCAAGCTGTTACTGTTATTCAAACAGTATTTTAATTACAAGAGATTATCCCTTTATTTTAGTTTGAATTTGTTGGTTTTAATTTGTTATCTGTTAGTTCTCAATACTTAGTCGGTCCCCCTTCGGCTAGTTGGGGTGTTTTGGTGGGCTGGTTGTTTTTGTATGCCCATGTATCCTTTTATTTTTTCTCAATGAAAGCAGGTTGTTTTATCAAAAGAAAAAAAAAAGAAGAAAAAAACAAATTAAAACTAAATTACAAGGATACCTAGTCATGCTACATCATACTTGTCTTGTGTGTTCCTGGATTGTGCATTTTCTTACTTTATTCTTTAGGTTTTTTTAGTAGACTGCAGACATTGGTTATTAATAATTTCTATGAAAGAGTTATTTTTAAATTTATTGGAAAATTTTAAAATATCTATTACATACCACTAGGTTCGCTATCATAATTGTTTAGCAATTCCTTGAGCATAAGCTAATAACTATAAAGTTCAGAAATTTATTGTTTATGCTTTTTTAACCAAATATGAAGTTGAAAGGTTTTGTTGAGTTTAGGCTTACATGTGGGAGAGTGAACAAAATTTTAGGCTTACGTTTAGAGGCATTTACTTTCTTATAAAGGAGAAAAATGAAAAAATAGTTTAATTAAAAGTTAAGACCTTTTAATATGGAATAAACATTTATCCATTATTTATTCATTTATTATAAGAAACTGAATATCATCAAAAGGATAAGTAAATGTTTATATGAATCCACATGTTTTAATATGTACTTAGGTATGTTTCTATAATTTCTTCAATTTTTTTTCTTTTTGAAATGAGATGGAAAATTTCCGTTGACAAAATGAAAAATTACATATAAGATGTTTGCAAGCTCCAAGTGTTTCTTGGCATACAATGCAAACAAGAAGAGAAGTACTATGTGAGAGCCATTGTTTAGAGGTATATTAGCACTATTGGTATGAATATGAGCAAGGGCATATTAGTAATAGCTAGTAAGTTGGTTAGGACTTTTAGTTACAAATAGAGGGAGTGGGATGGGGAGGAAGGTGTGGAGGTTTTAGTAAAATTTCCAGTGGAGAATTTGGGAGAGAGTAGCCCTCTTGATATTGCTCTTATATTTTAGTTTCTTCATTGATATTACAATTATATTTCTATCTTCCTATTTTTTTTATATTCTTTGTTGTTCTCGAGTGTTCTTGTTAGATGGGAACCCTAAATACTCAAATGTTTATAATCTATTGCAATTTTCTAATTAAAGAAATGAAAAAAACTACTGCATTATATATTGGCATCATTTTTTGAAGTTAATTAGCCATCTTATTTTTTTCTTTTCCAGTTCCAACTGATAAAATGTATGCGGATATTGAGATAAACTCTGATGAGACGGTTATCTTCTCGGAGACAAAATCCACATCTGTTGAAAAACATGAATGGTGCAAAATCACAAGGAATTCAGATCTAAATACTGCCACTCTGCAGAATAGAAGGTAATATGCTGTACAACAAAGTAATTAGTGTTTGAATCAAATGATATCCTTATCCCCATAAATCAAAGCTCCAATACGTTTTTGGTCCAGGCTTTTAAGATCTCATATAAAATTGTAAACCTTAGAAAAATTTTCCACTTTTACCCTTAAGGACTCTATTGGAGCTTTGGTATCAAAGTTCAGTAAACAAATACGATGAAGTTTCATGATTTTACACTGATAGCAGATTAGCTAGCTGATAGGAAGACCATGGGAGTTAGGTGTAAATTTCACCCAGGATCTTGACTTTTAGTGTTTAGGTAGAATGTTGATGAGAAAATTTTGTTTTCTCAATAATCTTGCTTTTATTTTTCAATGTTGTTGTAACCCTAAACCCTAATTTTTTTTGAAAAGGAAACCAGCCTCTTTATTGATAAGAATAACGTATTTCAAGGTACAAAAGGTTTATATTTTTTTTGAAATGGAGACAAGAATTTCTTTATTAAAAATAACTCAAAGTACAAGAGAGTTAGAGTCAGACAAAGAGAGCCAAAAAGAAATAGTAATCAAAGGAGACCAAGAGGGATTAGGAGGTGCACCCAGACATCTCAACTAGGTTGACACCGCCTTAGTGCTCTCACATTATATCCAACAAAAATAATACCCAAAGTACAAATTAAAGTCTGTCCAGAGTACAAACAACATGTGCTGTGGAGCCAAACAAAAACTACAAACAACATCGAAAAAACAGCAGCAGTACAAATCAGTAAAACAATTAATCTTTCAGTGGTGGCTGCAGACCACCATTCATGAAGAAAGTGACTACTAATGATTTGGTAAAATGA

mRNA sequence

AAAATTGTTTCATTTCAAAATTCTCAATCTTCTCCTTCTCCTACTGGCGGTATGGCGGAAATTGGAGAGTGCTCTGCTTCAAATTCATCCATGGAAAACTGGGCGAAGCTAATTCCAACTGATAAAATGTATGCGGATATTGAGATAAACTCTGATGAGACGGTTATCTTCTCGGAGACAAAATCCACATCTGTTGAAAAACATGAATGGTGCAAAATCACAAGGAATTCAGATCTAAATACTGCCACTCTGCAGAATAGAAGACCACCATTCATGAAGAAAGTGACTACTAATGATTTGGTAAAATGA

Coding sequence (CDS)

ATGGCGGAAATTGGAGAGTGCTCTGCTTCAAATTCATCCATGGAAAACTGGGCGAAGCTAATTCCAACTGATAAAATGTATGCGGATATTGAGATAAACTCTGATGAGACGGTTATCTTCTCGGAGACAAAATCCACATCTGTTGAAAAACATGAATGGTGCAAAATCACAAGGAATTCAGATCTAAATACTGCCACTCTGCAGAATAGAAGACCACCATTCATGAAGAAAGTGACTACTAATGATTTGGTAAAATGA

Protein sequence

MAEIGECSASNSSMENWAKLIPTDKMYADIEINSDETVIFSETKSTSVEKHEWCKITRNSDLNTATLQNRRPPFMKKVTTNDLVK*
Homology
[Close]
BLAST of CSPI04G20130 vs. ExPASy TrEMBL
Match: A0A0A0KYR0 (RING-type domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_4G508530 PE=4 SV=1)

HSP 1 Score: 142.5 bits (358), Expect = 8.2e-31
Identity = 70/70 (100.00%), Postives = 70/70 (100.00%), Query Frame = 0 

Query: 1  MAEIGECSASNSSMENWAKLIPTDKMYADIEINSDETVIFSETKSTSVEKHEWCKITRNS 60
          MAEIGECSASNSSMENWAKLIPTDKMYADIEINSDETVIFSETKSTSVEKHEWCKITRNS
Sbjct: 1  MAEIGECSASNSSMENWAKLIPTDKMYADIEINSDETVIFSETKSTSVEKHEWCKITRNS 60

Query: 61 DLNTATLQNR 71
          DLNTATLQNR
Sbjct: 61 DLNTATLQNR 70

[Close]
BLAST of CSPI04G20130 vs. ExPASy TrEMBL
Match: A0A1S4DUC8 (E3 ubiquitin-protein ligase CHFR isoform X3 OS=Cucumis melo OX=3656 GN=LOC103486049 PE=4 SV=1)

HSP 1 Score: 139.8 bits (351), Expect = 5.3e-30
Identity = 68/70 (97.14%), Postives = 69/70 (98.57%), Query Frame = 0 

Query: 1  MAEIGECSASNSSMENWAKLIPTDKMYADIEINSDETVIFSETKSTSVEKHEWCKITRNS 60
          MAEIGECSASNSS+ENWAKLIPTDKMY DIEINSDETVIFSETKSTSVEKHEWCKITRNS
Sbjct: 1  MAEIGECSASNSSLENWAKLIPTDKMYVDIEINSDETVIFSETKSTSVEKHEWCKITRNS 60

Query: 61 DLNTATLQNR 71
          DLNTATLQNR
Sbjct: 61 DLNTATLQNR 70

[Close]
BLAST of CSPI04G20130 vs. ExPASy TrEMBL
Match: A0A1S3B4G1 (E3 ubiquitin-protein ligase CHFR isoform X1 OS=Cucumis melo OX=3656 GN=LOC103486049 PE=4 SV=1)

HSP 1 Score: 139.8 bits (351), Expect = 5.3e-30
Identity = 68/70 (97.14%), Postives = 69/70 (98.57%), Query Frame = 0 

Query: 1  MAEIGECSASNSSMENWAKLIPTDKMYADIEINSDETVIFSETKSTSVEKHEWCKITRNS 60
          MAEIGECSASNSS+ENWAKLIPTDKMY DIEINSDETVIFSETKSTSVEKHEWCKITRNS
Sbjct: 1  MAEIGECSASNSSLENWAKLIPTDKMYVDIEINSDETVIFSETKSTSVEKHEWCKITRNS 60

Query: 61 DLNTATLQNR 71
          DLNTATLQNR
Sbjct: 61 DLNTATLQNR 70

[Close]
BLAST of CSPI04G20130 vs. ExPASy TrEMBL
Match: A0A1S3B4V7 (E3 ubiquitin-protein ligase CHFR isoform X2 OS=Cucumis melo OX=3656 GN=LOC103486049 PE=4 SV=1)

HSP 1 Score: 139.8 bits (351), Expect = 5.3e-30
Identity = 68/70 (97.14%), Postives = 69/70 (98.57%), Query Frame = 0 

Query: 1  MAEIGECSASNSSMENWAKLIPTDKMYADIEINSDETVIFSETKSTSVEKHEWCKITRNS 60
          MAEIGECSASNSS+ENWAKLIPTDKMY DIEINSDETVIFSETKSTSVEKHEWCKITRNS
Sbjct: 1  MAEIGECSASNSSLENWAKLIPTDKMYVDIEINSDETVIFSETKSTSVEKHEWCKITRNS 60

Query: 61 DLNTATLQNR 71
          DLNTATLQNR
Sbjct: 61 DLNTATLQNR 70

[Close]
BLAST of CSPI04G20130 vs. ExPASy TrEMBL
Match: A0A6J1HW48 (E3 ubiquitin-protein ligase CHFR isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111467202 PE=4 SV=1)

HSP 1 Score: 120.2 bits (300), Expect = 4.3e-24
Identity = 57/70 (81.43%), Postives = 65/70 (92.86%), Query Frame = 0 

Query: 1  MAEIGECSASNSSMENWAKLIPTDKMYADIEINSDETVIFSETKSTSVEKHEWCKITRNS 60
          MAE+GECSASNSS++NWAKLI  DK YADIEINSDE VIFSETKS+SV+K+EWCKITRNS
Sbjct: 1  MAEVGECSASNSSIQNWAKLISLDKKYADIEINSDEAVIFSETKSSSVDKYEWCKITRNS 60

Query: 61 DLNTATLQNR 71
          D+N+ TLQNR
Sbjct: 61 DVNSITLQNR 70

[Close]
BLAST of CSPI04G20130 vs. NCBI nr
Match: XP_004146401.2 (E3 ubiquitin-protein ligase CHFR isoform X1 [Cucumis sativus] >KAE8649715.1 hypothetical protein Csa_012000 [Cucumis sativus])

HSP 1 Score: 142.5 bits (358), Expect = 1.7e-30
Identity = 70/70 (100.00%), Postives = 70/70 (100.00%), Query Frame = 0 

Query: 1  MAEIGECSASNSSMENWAKLIPTDKMYADIEINSDETVIFSETKSTSVEKHEWCKITRNS 60
          MAEIGECSASNSSMENWAKLIPTDKMYADIEINSDETVIFSETKSTSVEKHEWCKITRNS
Sbjct: 1  MAEIGECSASNSSMENWAKLIPTDKMYADIEINSDETVIFSETKSTSVEKHEWCKITRNS 60

Query: 61 DLNTATLQNR 71
          DLNTATLQNR
Sbjct: 61 DLNTATLQNR 70

[Close]
BLAST of CSPI04G20130 vs. NCBI nr
Match: XP_031739669.1 (E3 ubiquitin-protein ligase CHFR isoform X3 [Cucumis sativus])

HSP 1 Score: 142.5 bits (358), Expect = 1.7e-30
Identity = 70/70 (100.00%), Postives = 70/70 (100.00%), Query Frame = 0 

Query: 1  MAEIGECSASNSSMENWAKLIPTDKMYADIEINSDETVIFSETKSTSVEKHEWCKITRNS 60
          MAEIGECSASNSSMENWAKLIPTDKMYADIEINSDETVIFSETKSTSVEKHEWCKITRNS
Sbjct: 1  MAEIGECSASNSSMENWAKLIPTDKMYADIEINSDETVIFSETKSTSVEKHEWCKITRNS 60

Query: 61 DLNTATLQNR 71
          DLNTATLQNR
Sbjct: 61 DLNTATLQNR 70

[Close]
BLAST of CSPI04G20130 vs. NCBI nr
Match: XP_031739668.1 (E3 ubiquitin-protein ligase CHFR isoform X2 [Cucumis sativus])

HSP 1 Score: 142.5 bits (358), Expect = 1.7e-30
Identity = 70/70 (100.00%), Postives = 70/70 (100.00%), Query Frame = 0 

Query: 1  MAEIGECSASNSSMENWAKLIPTDKMYADIEINSDETVIFSETKSTSVEKHEWCKITRNS 60
          MAEIGECSASNSSMENWAKLIPTDKMYADIEINSDETVIFSETKSTSVEKHEWCKITRNS
Sbjct: 1  MAEIGECSASNSSMENWAKLIPTDKMYADIEINSDETVIFSETKSTSVEKHEWCKITRNS 60

Query: 61 DLNTATLQNR 71
          DLNTATLQNR
Sbjct: 61 DLNTATLQNR 70

[Close]
BLAST of CSPI04G20130 vs. NCBI nr
Match: XP_008442086.1 (PREDICTED: E3 ubiquitin-protein ligase CHFR isoform X2 [Cucumis melo])

HSP 1 Score: 139.8 bits (351), Expect = 1.1e-29
Identity = 68/70 (97.14%), Postives = 69/70 (98.57%), Query Frame = 0 

Query: 1  MAEIGECSASNSSMENWAKLIPTDKMYADIEINSDETVIFSETKSTSVEKHEWCKITRNS 60
          MAEIGECSASNSS+ENWAKLIPTDKMY DIEINSDETVIFSETKSTSVEKHEWCKITRNS
Sbjct: 1  MAEIGECSASNSSLENWAKLIPTDKMYVDIEINSDETVIFSETKSTSVEKHEWCKITRNS 60

Query: 61 DLNTATLQNR 71
          DLNTATLQNR
Sbjct: 61 DLNTATLQNR 70

[Close]
BLAST of CSPI04G20130 vs. NCBI nr
Match: XP_016899584.1 (PREDICTED: E3 ubiquitin-protein ligase CHFR isoform X3 [Cucumis melo])

HSP 1 Score: 139.8 bits (351), Expect = 1.1e-29
Identity = 68/70 (97.14%), Postives = 69/70 (98.57%), Query Frame = 0 

Query: 1  MAEIGECSASNSSMENWAKLIPTDKMYADIEINSDETVIFSETKSTSVEKHEWCKITRNS 60
          MAEIGECSASNSS+ENWAKLIPTDKMY DIEINSDETVIFSETKSTSVEKHEWCKITRNS
Sbjct: 1  MAEIGECSASNSSLENWAKLIPTDKMYVDIEINSDETVIFSETKSTSVEKHEWCKITRNS 60

Query: 61 DLNTATLQNR 71
          DLNTATLQNR
Sbjct: 61 DLNTATLQNR 70

[Close]
BLAST of CSPI04G20130 vs. TAIR 10
Match: AT1G47570.1 (RING/U-box superfamily protein )

HSP 1 Score: 70.5 bits (171), Expect = 7.6e-13
Identity = 34/70 (48.57%), Postives = 50/70 (71.43%), Query Frame = 0 

Query: 2  AEIGECSASNSSMEN-WAKLIPTDKMYADIEINSDETVIFSETKSTSVEKHEWCKITRNS 61
          AE G+ S S  S ++ WAKL+P D  ++DIEI  ++ VI SE K +S+EKHEWC+IT+N 
Sbjct: 5  AETGQSSGSKPSDDDAWAKLVPLDTRFSDIEIRCNDMVICSEIKPSSLEKHEWCRITKNL 64

Query: 62 DLNTATLQNR 71
            ++AT+ N+
Sbjct: 65 GQSSATIHNK 74

[Close]
BLAST of CSPI04G20130 vs. TAIR 10
Match: AT1G47570.2 (RING/U-box superfamily protein )

HSP 1 Score: 55.8 bits (133), Expect = 1.9e-08
Identity = 24/50 (48.00%), Postives = 37/50 (74.00%), Query Frame = 0 

Query: 21 IPTDKMYADIEINSDETVIFSETKSTSVEKHEWCKITRNSDLNTATLQNR 71
          +P D  ++DIEI  ++ VI SE K +S+EKHEWC+IT+N   ++AT+ N+
Sbjct: 12 VPLDTRFSDIEIRCNDMVICSEIKPSSLEKHEWCRITKNLGQSSATIHNK 61

The following BLAST results are available for this feature:
BLAST of CSPI04G20130 vs.
Match NameE-valueIdentityDescription
BLAST of CSPI04G20130 vs. ExPASy TrEMBL
Match NameE-valueIdentityDescription
A0A0A0KYR08.2e-31100.00RING-type domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_4G508530 P... [more]
A0A1S4DUC85.3e-3097.14E3 ubiquitin-protein ligase CHFR isoform X3 OS=Cucumis melo OX=3656 GN=LOC103486... [more]
A0A1S3B4G15.3e-3097.14E3 ubiquitin-protein ligase CHFR isoform X1 OS=Cucumis melo OX=3656 GN=LOC103486... [more]
A0A1S3B4V75.3e-3097.14E3 ubiquitin-protein ligase CHFR isoform X2 OS=Cucumis melo OX=3656 GN=LOC103486... [more]
A0A6J1HW484.3e-2481.43E3 ubiquitin-protein ligase CHFR isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC11... [more]
BLAST of CSPI04G20130 vs. NCBI nr
Match NameE-valueIdentityDescription
XP_004146401.21.7e-30100.00E3 ubiquitin-protein ligase CHFR isoform X1 [Cucumis sativus] >KAE8649715.1 hypo... [more]
XP_031739669.11.7e-30100.00E3 ubiquitin-protein ligase CHFR isoform X3 [Cucumis sativus][more]
XP_031739668.11.7e-30100.00E3 ubiquitin-protein ligase CHFR isoform X2 [Cucumis sativus][more]
XP_008442086.11.1e-2997.14PREDICTED: E3 ubiquitin-protein ligase CHFR isoform X2 [Cucumis melo][more]
XP_016899584.11.1e-2997.14PREDICTED: E3 ubiquitin-protein ligase CHFR isoform X3 [Cucumis melo][more]
BLAST of CSPI04G20130 vs. TAIR 10
Match NameE-valueIdentityDescription
AT1G47570.17.6e-1348.57RING/U-box superfamily protein [more]
AT1G47570.21.9e-0848.00RING/U-box superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (PI 183967) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availablePANTHERPTHR16079UBIQUITIN LIGASE PROTEIN CHFRcoord: 3..71

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CSPI04G20130.1CSPI04G20130.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0016567 protein ubiquitination
biological_process GO:0006511 ubiquitin-dependent protein catabolic process
cellular_component GO:0005634 nucleus
molecular_function GO:0046872 metal ion binding
molecular_function GO:0004842 ubiquitin-protein transferase activity