CSPI04G17710 (gene) Cucumber (PI 183967) v1

Overview
NameCSPI04G17710
Typegene
OrganismCucumis sativus var. hardwickii cv. PI 183967 (Cucumber (PI 183967) v1)
Descriptionaspartic proteinase Asp1-like
LocationChr4: 15162167 .. 15165260 (-)
RNA-Seq ExpressionCSPI04G17710
SyntenyCSPI04G17710
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
CTTTCTTATCTTCATCCTCCTTTGTCCTCTTCTCCAACAACGACCTCAAGTCGTCGCTCTAATTCTTCGCTTCGCCATTAACTTCTTTACCTTCCACCTTTCATTTTCATTCTTCTCTACACATTTTTACTATGAATTCTCACTTCTCTATTTATGGACATTCAATTTCACTCTCCATTGTTCTGATCTTCATCTCTTCACTCTTCCCATACCCTTCTTTCTCCTTTCCGATTCGTATCCATTCCAGGATTCGCGACCCACTTCTGTTTTTCGCTGTAAAACAACGCAGATGCCCACTCTGTTGCTTGTCTCGATTCTCTTTGCGAGTTTCGCCGTTTCCTTGTCCGACAAGTTTCTCTTCGCCGATTCGGAGCAAGTTAAGACCCTTCGGTTTGGTTCGTCGGTTTTGTTTCCTGTTAGAGGAAATGTTTACCCTCTAGGGTATGTGTTTTGTGTTATGATGTTTTCATTTTGATTTTGTTGTACTGTTTGGATTGATAGATTGTTGGAATCTGCAGGCATTTTACGGTGTTACTCAATATTGGTAATCCGTCTAAGGTTTTTGAGCTTGATATTGACACTGGGAGTGATCTCACTTGGGTTCAATGTGATGTTGAATGTATTGGTTGCACTTTGGTATGTTGGATCCTTTTCTTAATCGATTTTGATTCGTTGTTACCATGTTTTTTTGTAGCGTAACTTGGTATTAACTGTTATTTTTTTAGCCTCGTGATATGCTCTATAGACCACATAATAACGCTGTGAGTCGTGAAGACCCATTATGTGCGGCGCTTTCTTCACTGGGTAAGTTCATTTTCAAGAACCCAAATGATCAATGTGCCTACGAGGTTGAGTATGCCGATCATGGATCATCCGTTGGTGTATTGGTCAAAGATCTTGTTCCTATGAGACTCACTAATGGCAAAAGAATTAGTCCCAATTTGGGCTTTGGGTGTGTATACAAAATATCACTCTCCTTTGCAGAGACAATTCTGCTTTATAACTCTGGTTTCGTTTTTCGTATTTTGTTTTTTAAAGCTATGCTTACTTTCCCGCTATTTCTTATAGCATTTTTCGATTACTTAAAATCATAATTGGATTTATACCTTAGATGGTAAAATCGTTTCTAAAAAGTAGGTAACGAAAGTAATCTTTATTGGAAGTACAAGTAGCTTTAATTTAAGAAAACAGACCAAACTTGGGTTTCATTTGTTATCCATTTTGTCTTTAATATTATTTTCACTTTGTTAAAGAAATACTTAGAATCACTAGTTGAATTCTAAAATAAAAGCTGTTGGTTTTTTAAAACATAAGTAGGAAGTGAATAAAAAAATATATTAGAGGTGAGATTAGTGTTCATTGACTTAATTTTCAAAAACAACAAACAAAAGGATATCTGAACTTTAATGGTTATAAGGAGGGATTAAACTTTTTGGCGGTTGAATAATCGGTTCTGCTAGGCTTACACAGTGCCCTTGTTCTTTGAAACTTGTTACCAATCTTTCTTTCCTCTGATTCCTCTTGCCCTGCAGTTGCGGATACGATCAGGAAAATGGTGATTTGCAACAGCCTCCTTCAATAGCTGGTGTCCTTGGCCTTAGTAGTAGTAAAGCTACAATTGTATCACAGCTAAGCGACCTCGGTCATGTAAGCAACGTAGTTGGACACTGCCTCACTGGACGTGGAGAAGGATTTCTATTCTTTGGTGGAGACGTTGTCCCATCTTCAGGGATGTCGTGGACACCAATATTGCGCAATTCAGAAGGGTAAGTGGGCAATGTGCAGTCAGATATTTGCTTCTTAAACGTCTGTGTTGTTGACATGCCATGTATTTTTTATTTGATCTCATTCATTCTCAGGAAGTACTCATCTGGACCTGCAGAGGTCTACTTTAATGGGAGAGCTGTTGGAATTGGAGGCCTCACCCTGACTTTTGATAGTGGAAGCTCTTACACTTATTTCAACTCCCAAGTCTATCGAGCAATAGAGAAACTGGTGAGAATATGAACAGAATTAACAAGAATTTTCTTGAATGGTATTATTGGAAGTAGGATCTTGTAGGAAGAAAATAATGATATGTTATGACATTGACATCCCAGTTGAAGAATGATTTGAAAGGAAAGCCTCTGAAACTTGCATCTGATGATAAAACCCTTGAACTGTGCTGGAAAGGTCCAAAACCTTTTGAATCTGTGGTCGATGTGAGAAACTTCTTCAAGCCCCTGGCCATGAGCTTTAAAAACTCCAAGAATGTTCAATTTCAAATACCTCCTGAAGCTTATCTCATAATCAGTGTAAGTTTCTCAAACATCTCATTAAACCATCCACATATTGAGGAACAAAATAAGATTTGTGGATTAGGTTGTAAGAAGTTCTTTCTTTTACAGGAATTTGGTAATGTGTGCTTGGGGATTTTGGATGGAAGCAAAGAAGGAATGGGAAATGTTAACATAATCGGAGGTAAGTATCCATTGGGATTGACTAGATAAATGTGTTCTTCCAAATGTCATTTTTATTTAAACATTTTTTTTATAAAACAGTTTAAAATACACTTGTTTTAAAAACTATGTTAAGTAGTTATCAAACATTTTAATTCTTTACAAAGATGATTTATTTTCAAAACTAAATATCCAAACAATAGGATTTCAACTCATTTTTACCTTTTTTTTATCTGTTTTTGCTTCCTTAAATTCATGGACAGATATTTCTATGCTGAATAAGATTGTAGTCTACGACAATGAGCGAGAGAGGATTGGATGGGCCTCTTCTAATTGCAATAGATCTCCTAGAAATGAAGCTTCTTGGTACGTGAGATTTTCATTTGAAAAATACAAAGTGCTTTTTGAACATCAACTTTACTTTTCATCGTCTTATATTAAGAAATTTTTACATTTTAGGTGAGCATTAACCGGCTTAGTGGAGAAAGTTATTATCATTCAACCCAATAGATAGATCAAGAGTTGGGGAATTATATAATATATTAATGAATAATGACTAATAGTTTGTGTTTGTATTTGAACTGAAGAGTTACAAGTTAGACATATGATAACATCTATTCTCTCTTTTTTATTTTAACAATGGTAAATGACTGATT

mRNA sequence

CTTTCTTATCTTCATCCTCCTTTGTCCTCTTCTCCAACAACGACCTCAAGTCGTCGCTCTAATTCTTCGCTTCGCCATTAACTTCTTTACCTTCCACCTTTCATTTTCATTCTTCTCTACACATTTTTACTATGAATTCTCACTTCTCTATTTATGGACATTCAATTTCACTCTCCATTGTTCTGATCTTCATCTCTTCACTCTTCCCATACCCTTCTTTCTCCTTTCCGATTCGTATCCATTCCAGGATTCGCGACCCACTTCTGTTTTTCGCTGTAAAACAACGCAGATGCCCACTCTGTTGCTTGTCTCGATTCTCTTTGCGAGTTTCGCCGTTTCCTTGTCCGACAAGTTTCTCTTCGCCGATTCGGAGCAAGTTAAGACCCTTCGGTTTGGTTCGTCGGTTTTGTTTCCTGTTAGAGGAAATGTTTACCCTCTAGGGCATTTTACGGTGTTACTCAATATTGGTAATCCGTCTAAGGTTTTTGAGCTTGATATTGACACTGGGAGTGATCTCACTTGGGTTCAATGTGATGTTGAATGTATTGGTTGCACTTTGCCTCGTGATATGCTCTATAGACCACATAATAACGCTGTGAGTCGTGAAGACCCATTATGTGCGGCGCTTTCTTCACTGGGTAAGTTCATTTTCAAGAACCCAAATGATCAATGTGCCTACGAGGTTGAGTATGCCGATCATGGATCATCCGTTGGTGTATTGGTCAAAGATCTTGTTCCTATGAGACTCACTAATGGCAAAAGAATTAGTCCCAATTTGGGCTTTGGTTGCGGATACGATCAGGAAAATGGTGATTTGCAACAGCCTCCTTCAATAGCTGGTGTCCTTGGCCTTAGTAGTAGTAAAGCTACAATTGTATCACAGCTAAGCGACCTCGGTCATGTAAGCAACGTAGTTGGACACTGCCTCACTGGACGTGGAGAAGGATTTCTATTCTTTGGTGGAGACGTTGTCCCATCTTCAGGGATGTCGTGGACACCAATATTGCGCAATTCAGAAGGGAAGTACTCATCTGGACCTGCAGAGGTCTACTTTAATGGGAGAGCTGTTGGAATTGGAGGCCTCACCCTGACTTTTGATAGTGGAAGCTCTTACACTTATTTCAACTCCCAAGTCTATCGAGCAATAGAGAAACTGTTGAAGAATGATTTGAAAGGAAAGCCTCTGAAACTTGCATCTGATGATAAAACCCTTGAACTGTGCTGGAAAGGTCCAAAACCTTTTGAATCTGTGGTCGATGTGAGAAACTTCTTCAAGCCCCTGGCCATGAGCTTTAAAAACTCCAAGAATGTTCAATTTCAAATACCTCCTGAAGCTTATCTCATAATCAGTGAATTTGGTAATGTGTGCTTGGGGATTTTGGATGGAAGCAAAGAAGGAATGGGAAATGTTAACATAATCGGAGATATTTCTATGCTGAATAAGATTGTAGTCTACGACAATGAGCGAGAGAGGATTGGATGGGCCTCTTCTAATTGCAATAGATCTCCTAGAAATGAAGCTTCTTGGTGAGCATTAACCGGCTTAGTGGAGAAAGTTATTATCATTCAACCCAATAGATAGATCAAGAGTTGGGGAATTATATAATATATTAATGAATAATGACTAATAGTTTGTGTTTGTATTTGAACTGAAGAGTTACAAGTTAGACATATGATAACATCTATTCTCTCTTTTTTATTTTAACAATGGTAAATGACTGATT

Coding sequence (CDS)

ATGCCCACTCTGTTGCTTGTCTCGATTCTCTTTGCGAGTTTCGCCGTTTCCTTGTCCGACAAGTTTCTCTTCGCCGATTCGGAGCAAGTTAAGACCCTTCGGTTTGGTTCGTCGGTTTTGTTTCCTGTTAGAGGAAATGTTTACCCTCTAGGGCATTTTACGGTGTTACTCAATATTGGTAATCCGTCTAAGGTTTTTGAGCTTGATATTGACACTGGGAGTGATCTCACTTGGGTTCAATGTGATGTTGAATGTATTGGTTGCACTTTGCCTCGTGATATGCTCTATAGACCACATAATAACGCTGTGAGTCGTGAAGACCCATTATGTGCGGCGCTTTCTTCACTGGGTAAGTTCATTTTCAAGAACCCAAATGATCAATGTGCCTACGAGGTTGAGTATGCCGATCATGGATCATCCGTTGGTGTATTGGTCAAAGATCTTGTTCCTATGAGACTCACTAATGGCAAAAGAATTAGTCCCAATTTGGGCTTTGGTTGCGGATACGATCAGGAAAATGGTGATTTGCAACAGCCTCCTTCAATAGCTGGTGTCCTTGGCCTTAGTAGTAGTAAAGCTACAATTGTATCACAGCTAAGCGACCTCGGTCATGTAAGCAACGTAGTTGGACACTGCCTCACTGGACGTGGAGAAGGATTTCTATTCTTTGGTGGAGACGTTGTCCCATCTTCAGGGATGTCGTGGACACCAATATTGCGCAATTCAGAAGGGAAGTACTCATCTGGACCTGCAGAGGTCTACTTTAATGGGAGAGCTGTTGGAATTGGAGGCCTCACCCTGACTTTTGATAGTGGAAGCTCTTACACTTATTTCAACTCCCAAGTCTATCGAGCAATAGAGAAACTGTTGAAGAATGATTTGAAAGGAAAGCCTCTGAAACTTGCATCTGATGATAAAACCCTTGAACTGTGCTGGAAAGGTCCAAAACCTTTTGAATCTGTGGTCGATGTGAGAAACTTCTTCAAGCCCCTGGCCATGAGCTTTAAAAACTCCAAGAATGTTCAATTTCAAATACCTCCTGAAGCTTATCTCATAATCAGTGAATTTGGTAATGTGTGCTTGGGGATTTTGGATGGAAGCAAAGAAGGAATGGGAAATGTTAACATAATCGGAGATATTTCTATGCTGAATAAGATTGTAGTCTACGACAATGAGCGAGAGAGGATTGGATGGGCCTCTTCTAATTGCAATAGATCTCCTAGAAATGAAGCTTCTTGGTGA

Protein sequence

MPTLLLVSILFASFAVSLSDKFLFADSEQVKTLRFGSSVLFPVRGNVYPLGHFTVLLNIGNPSKVFELDIDTGSDLTWVQCDVECIGCTLPRDMLYRPHNNAVSREDPLCAALSSLGKFIFKNPNDQCAYEVEYADHGSSVGVLVKDLVPMRLTNGKRISPNLGFGCGYDQENGDLQQPPSIAGVLGLSSSKATIVSQLSDLGHVSNVVGHCLTGRGEGFLFFGGDVVPSSGMSWTPILRNSEGKYSSGPAEVYFNGRAVGIGGLTLTFDSGSSYTYFNSQVYRAIEKLLKNDLKGKPLKLASDDKTLELCWKGPKPFESVVDVRNFFKPLAMSFKNSKNVQFQIPPEAYLIISEFGNVCLGILDGSKEGMGNVNIIGDISMLNKIVVYDNERERIGWASSNCNRSPRNEASW*
Homology
BLAST of CSPI04G17710 vs. ExPASy Swiss-Prot
Match: Q0IU52 (Aspartic proteinase Asp1 OS=Oryza sativa subsp. japonica OX=39947 GN=ASP1 PE=2 SV=1)

HSP 1 Score: 312.4 bits (799), Expect = 7.8e-84
Identity = 161/387 (41.60%), Postives = 242/387 (62.53%), Query Frame = 0

Query: 37  SSVLFPVRGNVYPLGHFTVLLNIGNPSKVFELDIDTGSDLTWVQCDVECIGCTLPRDMLY 96
           S+V+  + GNVYP+GHF + +NIG+P+K + LDIDTGS LTW+QCD  C  C +   +LY
Sbjct: 22  SAVVLELHGNVYPIGHFFITMNIGDPAKSYFLDIDTGSTLTWLQCDAPCTNCNIVPHVLY 81

Query: 97  RP-HNNAVSREDPLCAAL-SSLGKFIFKNPNDQCAYEVEYADHGSSVGVLVKDLVPMRLT 156
           +P     V+  D LC  L + LGK        QC Y ++Y D  SS+GVLV D   +  +
Sbjct: 82  KPTPKKLVTCADSLCTDLYTDLGKPKRCGSQKQCDYVIQYVD-SSSMGVLVIDRFSLSAS 141

Query: 157 NGKRISPNLGFGCGYDQENGDLQQPPSIAGVLGLSSSKATIVSQLSDLGHVS-NVVGHCL 216
           NG      + FGCGYDQ   +   P  +  +LGLS  K T++SQL   G ++ +V+GHC+
Sbjct: 142 NGTN-PTTIAFGCGYDQGKKNRNVPIPVDSILGLSRGKVTLLSQLKSQGVITKHVLGHCI 201

Query: 217 TGRGEGFLFFGGDVVPSSGMSWTPILRNSEGKYSSGPAEVYF--NGRAVGIGGLTLTFDS 276
           + +G GFLFFG   VP+SG++WTP+ R  +  YS G   ++F  N +A+    + + FDS
Sbjct: 202 SSKGGGFLFFGDAQVPTSGVTWTPMNREHK-YYSPGHGTLHFDSNSKAISAAPMAVIFDS 261

Query: 277 GSSYTYFNSQVYRAIEKLLKNDLKGK---PLKLASDDKTLELCWKGPKPFESVVDVRNFF 336
           G++YTYF +Q Y+A   ++K+ L  +     ++   D+ L +CWKG     ++ +V+  F
Sbjct: 262 GATYTYFAAQPYQATLSVVKSTLNSECKFLTEVTEKDRALTVCWKGKDKIVTIDEVKKCF 321

Query: 337 KPLAMSFKN-SKNVQFQIPPEAYLIISEFGNVCLGILDGSKE--GMGNVNIIGDISMLNK 396
           + L++ F +  K    +IPPE YLIIS+ G+VCLGILDGSKE   +   N+IG I+ML++
Sbjct: 322 RSLSLEFADGDKKATLEIPPEHYLIISQEGHVCLGILDGSKEHLSLAGTNLIGGITMLDQ 381

Query: 397 IVVYDNERERIGWASSNCNRSPRNEAS 413
           +V+YD+ER  +GW +  C+R PR+E++
Sbjct: 382 MVIYDSERSLLGWVNYQCDRIPRSESA 405

BLAST of CSPI04G17710 vs. ExPASy Swiss-Prot
Match: A2ZC67 (Aspartic proteinase Asp1 OS=Oryza sativa subsp. indica OX=39946 GN=ASP1 PE=2 SV=2)

HSP 1 Score: 303.9 bits (777), Expect = 2.8e-81
Identity = 160/389 (41.13%), Postives = 241/389 (61.95%), Query Frame = 0

Query: 37  SSVLFPVRGNVYPLGHFTVLLNIGNPSKVFELDIDTGSDLTWVQCDVECIGCTLPRDMLY 96
           S+V+  + GNVYP+GHF V +NIG+P+K + LDIDTGS LTW+QCD  CI C      LY
Sbjct: 22  SAVVLELHGNVYPIGHFFVTMNIGDPAKPYFLDIDTGSTLTWLQCDYPCINCNKVPHGLY 81

Query: 97  RPH-NNAVSREDPLCAAL-SSLGKFIFKNPNDQCAYEVEYADHGSSVGVLVKDLVPMRLT 156
           +P    AV   +  CA L + L K +   P +QC Y ++Y   GSS+GVL+ D   +  +
Sbjct: 82  KPELKYAVKCTEQRCADLYADLRKPMKCGPKNQCHYGIQYVG-GSSIGVLIVDSFSLPAS 141

Query: 157 NGKRISPNLGFGCGYDQENGDLQQPPSIAGVLGLSSSKATIVSQLSDLGHVS-NVVGHCL 216
           NG     ++ FGCGY+Q   +   P  + G+LGL   K T++SQL   G ++ +V+GHC+
Sbjct: 142 NGTN-PTSIAFGCGYNQGKNNHNVPTPVNGILGLGRGKVTLLSQLKSQGVITKHVLGHCI 201

Query: 217 TGRGEGFLFFGGDVVPSSGMSWTPILRNSEGKYSS---GPAEVYFNGRAVGIGGLTLTFD 276
           + +G+GFLFFG   VP+SG++W+P+  N E K+ S   G  +   N + +    + + FD
Sbjct: 202 SSKGKGFLFFGDAKVPTSGVTWSPM--NREHKHYSPRQGTLQFNSNSKPISAAPMEVIFD 261

Query: 277 SGSSYTYFNSQVYRAIEKLLKNDLKGKPLKLASD----DKTLELCWKGPKPFESVVDVRN 336
           SG++YTYF  Q Y A   ++K+ L  K  K  ++    D+ L +CWKG     ++ +V+ 
Sbjct: 262 SGATYTYFALQPYHATLSVVKSTL-SKECKFLTEVKEKDRALTVCWKGKDKIRTIDEVKK 321

Query: 337 FFKPLAMSFKN-SKNVQFQIPPEAYLIISEFGNVCLGILDGSKE--GMGNVNIIGDISML 396
            F+ L++ F +  K    +IPPE YLIIS+ G+VCLGILDGSKE   +   N+IG I+ML
Sbjct: 322 CFRSLSLKFADGDKKATLEIPPEHYLIISQEGHVCLGILDGSKEHPSLAGTNLIGGITML 381

Query: 397 NKIVVYDNERERIGWASSNCNRSPRNEAS 413
           +++V+YD+ER  +GW +  C+R PR+ ++
Sbjct: 382 DQMVIYDSERSLLGWVNYQCDRIPRSASA 405

BLAST of CSPI04G17710 vs. ExPASy Swiss-Prot
Match: Q9M9A8 (Aspartyl protease APCB1 OS=Arabidopsis thaliana OX=3702 GN=APCB1 PE=1 SV=1)

HSP 1 Score: 297.4 bits (760), Expect = 2.6e-79
Identity = 161/387 (41.60%), Postives = 221/387 (57.11%), Query Frame = 0

Query: 37  SSVLFPVRGNVYPLGHFTVLLNIGNP--SKVFELDIDTGSDLTWVQCDVECIGCTLPRDM 96
           S+ +FPV GNVYP G +   + +G P   + + LDIDTGS+LTW+QCD  C  C    + 
Sbjct: 187 STTIFPVGGNVYPDGLYYTRILVGKPEDGQYYHLDIDTGSELTWIQCDAPCTSCAKGANQ 246

Query: 97  LYRPH-NNAVSREDPLCAALSSLGKFIFKNPNDQCAYEVEYADHGSSVGVLVKDLVPMRL 156
           LY+P  +N V   +  C  +             QC YE+EYADH  S+GVL KD   ++L
Sbjct: 247 LYKPRKDNLVRSSEAFCVEVQRNQLTEHCENCHQCDYEIEYADHSYSMGVLTKDKFHLKL 306

Query: 157 TNGKRISPNLGFGCGYDQENGDLQQPPSIAGVLGLSSSKATIVSQLSDLGHVSNVVGHCL 216
            NG     ++ FGCGYDQ+   L       G+LGLS +K ++ SQL+  G +SNVVGHCL
Sbjct: 307 HNGSLAESDIVFGCGYDQQGLLLNTLLKTDGILGLSRAKISLPSQLASRGIISNVVGHCL 366

Query: 217 TG--RGEGFLFFGGDVVPSSGMSWTPILRNSE--------GKYSSGPAEVYFNGRAVGIG 276
                GEG++F G D+VPS GM+W P+L +S          K S G   +  +G    +G
Sbjct: 367 ASDLNGEGYIFMGSDLVPSHGMTWVPMLHDSRLDAYQMQVTKMSYGQGMLSLDGENGRVG 426

Query: 277 GLTLTFDSGSSYTYFNSQVYRAIEKLLKNDLKGKPLKLASDDKTLELCWKGPK--PFESV 336
              + FD+GSSYTYF +Q Y  +   L+ ++ G  L     D+TL +CW+     PF S+
Sbjct: 427 --KVLFDTGSSYTYFPNQAYSQLVTSLQ-EVSGLELTRDDSDETLPICWRAKTNFPFSSL 486

Query: 337 VDVRNFFKPLAMSFKNSKNV---QFQIPPEAYLIISEFGNVCLGILDGSKEGMGNVNIIG 396
            DV+ FF+P+ +   +   +   +  I PE YLIIS  GNVCLGILDGS    G+  I+G
Sbjct: 487 SDVKKFFRPITLQIGSKWLIISRKLLIQPEDYLIISNKGNVCLGILDGSSVHDGSTIILG 546

Query: 397 DISMLNKIVVYDNERERIGWASSNCNR 406
           DISM   ++VYDN + RIGW  S+C R
Sbjct: 547 DISMRGHLIVYDNVKRRIGWMKSDCVR 570

BLAST of CSPI04G17710 vs. ExPASy Swiss-Prot
Match: Q9S9K4 (Aspartic proteinase 39 OS=Arabidopsis thaliana OX=3702 GN=A39 PE=1 SV=2)

HSP 1 Score: 146.0 bits (367), Expect = 9.6e-34
Identity = 117/444 (26.35%), Postives = 195/444 (43.92%), Query Frame = 0

Query: 4   LLLVSILFASFAVSLSDKFL--------FADSEQVKTLRFGSSVLFPVRGN--VYPLGHF 63
           ++++    A+F      KF         F   +  +  R  +S+  P+ G+  V  +G +
Sbjct: 15  VIVIEFASANFVFKAQHKFAGKKKNLEHFKSHDTRRHSRMLASIDLPLGGDSRVDSVGLY 74

Query: 64  TVLLNIGNPSKVFELDIDTGSDLTWVQCDVECIGCTLPRDMLYR---------PHNNAVS 123
              + +G+P K + + +DTGSD+ W+ C   C  C    ++ +R           +  V 
Sbjct: 75  FTKIKLGSPPKEYHVQVDTGSDILWINCK-PCPKCPTKTNLNFRLSLFDMNASSTSKKVG 134

Query: 124 REDPLCAALSSLGKFIFKNPNDQCAYEVEYADHGSSVGVLVKDLVPMRLTNGKRISPNLG 183
            +D  C+ +S         P   C+Y + YAD  +S G  ++D++ +    G   +  LG
Sbjct: 135 CDDDFCSFISQSDSC---QPALGCSYHIVYADESTSDGKFIRDMLTLEQVTGDLKTGPLG 194

Query: 184 ----FGCGYDQE----NGDLQQPPSIAGVLGLSSSKATIVSQLSDLGHVSNVVGHCLTGR 243
               FGCG DQ     NGD     ++ GV+G   S  +++SQL+  G    V  HCL   
Sbjct: 195 QEVVFGCGSDQSGQLGNGD----SAVDGVMGFGQSNTSVLSQLAATGDAKRVFSHCLDNV 254

Query: 244 GEGFLFFGGDVVPSSGMSWTPILRNS------------EGKYSSGPAEVYFNGRAVGIGG 303
             G +F  G VV S  +  TP++ N             +G     P  +  NG  +    
Sbjct: 255 KGGGIFAVG-VVDSPKVKTTPMVPNQMHYNVMLMGMDVDGTSLDLPRSIVRNGGTI---- 314

Query: 304 LTLTFDSGSSYTYFNSQVYRAIEKLLKNDLKGKPLKLASDDKTLELCWKGPKPFESVVDV 363
                DSG++  YF   +Y   + L++  L  +P+KL   ++T +        F    +V
Sbjct: 315 ----VDSGTTLAYFPKVLY---DSLIETILARQPVKLHIVEETFQC-------FSFSTNV 374

Query: 364 RNFFKPLAMSFKNSKNVQFQIPPEAYLIISEFGNVCLGILDG--SKEGMGNVNIIGDISM 407
              F P++  F++S  V+  + P  YL   E    C G   G  + +    V ++GD+ +
Sbjct: 375 DEAFPPVSFEFEDS--VKLTVYPHDYLFTLEEELYCFGWQAGGLTTDERSEVILLGDLVL 429

BLAST of CSPI04G17710 vs. ExPASy Swiss-Prot
Match: Q4V3D2 (Aspartic proteinase 36 OS=Arabidopsis thaliana OX=3702 GN=A36 PE=1 SV=1)

HSP 1 Score: 131.7 bits (330), Expect = 1.9e-29
Identity = 112/437 (25.63%), Postives = 197/437 (45.08%), Query Frame = 0

Query: 4   LLLVSILFASFAVSLSDKFL--------FADSEQVKTLRFGSSVLFPVRGN--VYPLGHF 63
           +L++ ++  +F  +++ KF             +  +  R  +++  P+ G+     +G +
Sbjct: 19  VLVIQVVSGNFVFNVTHKFAGKEKQLSELKSHDSFRHARMLANIDLPLGGDSRADSIGLY 78

Query: 64  TVLLNIGNPSKVFELDIDTGSDLTWVQCDVECIGCTLPRDM-----LYRPHNNAVSR--- 123
              + +G+P K + + +DTGSD+ WV C   C  C +  D+     LY    ++ S+   
Sbjct: 79  FTKIKLGSPPKEYYVQVDTGSDILWVNC-APCPKCPVKTDLGIPLSLYDSKTSSTSKNVG 138

Query: 124 -EDPLCAALSSLGKFIFKNPNDQCAYEVEYADHGSSVGVLVKDLVPM-RLTNGKRISP-- 183
            ED  C+ +        K P   C+Y V Y D  +S G  +KD + + ++T   R +P  
Sbjct: 139 CEDDFCSFIMQSETCGAKKP---CSYHVVYGDGSTSDGDFIKDNITLEQVTGNLRTAPLA 198

Query: 184 -NLGFGCGYDQENGDLQQPPSIAGVLGLSSSKATIVSQLSDLGHVSNVVGHCLTGRGEGF 243
             + FGCG +Q     Q   ++ G++G   S  +I+SQL+  G    +  HCL     G 
Sbjct: 199 QEVVFGCGKNQSGQLGQTDSAVDGIMGFGQSNTSIISQLAAGGSTKRIFSHCLDNMNGGG 258

Query: 244 LFFGGDVVPSSGMSWTPILRNS-------EGKYSSG-PAEVYFNGRAVGIGGLTLTFDSG 303
           +F  G+ V S  +  TPI+ N        +G    G P ++  +  +    G T+  DSG
Sbjct: 259 IFAVGE-VESPVVKTTPIVPNQVHYNVILKGMDVDGDPIDLPPSLASTNGDGGTI-IDSG 318

Query: 304 SSYTYFNSQVYRA-IEKLLKNDLKGKPLKLASDDKTLELCWKGPKPFESVVDVRNFFKPL 363
           ++  Y    +Y + IEK+       + +KL    +T          F    +    F  +
Sbjct: 319 TTLAYLPQNLYNSLIEKITAK----QQVKLHMVQETFAC-------FSFTSNTDKAFPVV 378

Query: 364 AMSFKNSKNVQFQIPPEAYLIISEFGNVCLGILDG--SKEGMGNVNIIGDISMLNKIVVY 407
            + F++S  ++  + P  YL        C G   G  + +   +V ++GD+ + NK+VVY
Sbjct: 379 NLHFEDS--LKLSVYPHDYLFSLREDMYCFGWQSGGMTTQDGADVILLGDLVLSNKLVVY 436

BLAST of CSPI04G17710 vs. ExPASy TrEMBL
Match: A0A0A0L1V4 (Peptidase A1 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_4G361860 PE=4 SV=1)

HSP 1 Score: 842.8 bits (2176), Expect = 6.1e-241
Identity = 411/413 (99.52%), Postives = 411/413 (99.52%), Query Frame = 0

Query: 1   MPTLLLVSILFASFAVSLSDKFLFADSEQVKTLRFGSSVLFPVRGNVYPLGHFTVLLNIG 60
           MPTLLLVSILFASFAVSLSDKFLFADSEQVKTLRFGSSVLFPVRGNVYPLGHFTVLLNIG
Sbjct: 1   MPTLLLVSILFASFAVSLSDKFLFADSEQVKTLRFGSSVLFPVRGNVYPLGHFTVLLNIG 60

Query: 61  NPSKVFELDIDTGSDLTWVQCDVECIGCTLPRDMLYRPHNNAVSREDPLCAALSSLGKFI 120
           NPSKVFELDIDTGSDLTWVQCDVECIGCTLPRDMLYRPHNNAVSREDPLCAALSSLGKFI
Sbjct: 61  NPSKVFELDIDTGSDLTWVQCDVECIGCTLPRDMLYRPHNNAVSREDPLCAALSSLGKFI 120

Query: 121 FKNPNDQCAYEVEYADHGSSVGVLVKDLVPMRLTNGKRISPNLGFGCGYDQENGDLQQPP 180
           FKNPNDQCAYEVEYADHGSSVGVLVKDLVPMRLTNGKRISPNLGFGCGYDQENGDLQQPP
Sbjct: 121 FKNPNDQCAYEVEYADHGSSVGVLVKDLVPMRLTNGKRISPNLGFGCGYDQENGDLQQPP 180

Query: 181 SIAGVLGLSSSKATIVSQLSDLGHVSNVVGHCLTGRGEGFLFFGGDVVPSSGMSWTPILR 240
           SIAGVLGLSSSKATIVSQLSDLGHVSNVVGHCLTGRG GFLFFGGDVVPSSGMSWTPILR
Sbjct: 181 SIAGVLGLSSSKATIVSQLSDLGHVSNVVGHCLTGRGGGFLFFGGDVVPSSGMSWTPILR 240

Query: 241 NSEGKYSSGPAEVYFNGRAVGIGGLTLTFDSGSSYTYFNSQVYRAIEKLLKNDLKGKPLK 300
           NSEGKYSSGPAEVYFNGRAVGIGGLTLTFDSGSSYTYFNSQVYRAIEKLLKNDLKG PLK
Sbjct: 241 NSEGKYSSGPAEVYFNGRAVGIGGLTLTFDSGSSYTYFNSQVYRAIEKLLKNDLKGNPLK 300

Query: 301 LASDDKTLELCWKGPKPFESVVDVRNFFKPLAMSFKNSKNVQFQIPPEAYLIISEFGNVC 360
           LASDDKTLELCWKGPKPFESVVDVRNFFKPLAMSFKNSKNVQFQIPPEAYLIISEFGNVC
Sbjct: 301 LASDDKTLELCWKGPKPFESVVDVRNFFKPLAMSFKNSKNVQFQIPPEAYLIISEFGNVC 360

Query: 361 LGILDGSKEGMGNVNIIGDISMLNKIVVYDNERERIGWASSNCNRSPRNEASW 414
           LGILDGSKEGMGNVNIIGDISMLNKIVVYDNERERIGWASSNCNRSPRNEASW
Sbjct: 361 LGILDGSKEGMGNVNIIGDISMLNKIVVYDNERERIGWASSNCNRSPRNEASW 413

BLAST of CSPI04G17710 vs. ExPASy TrEMBL
Match: A0A1S3B9B9 (aspartic proteinase Asp1-like OS=Cucumis melo OX=3656 GN=LOC103487600 PE=4 SV=1)

HSP 1 Score: 787.3 bits (2032), Expect = 3.0e-224
Identity = 381/413 (92.25%), Postives = 396/413 (95.88%), Query Frame = 0

Query: 1   MPTLLLVSILFASFAVSLSDKFLFADSEQVKTLRFGSSVLFPVRGNVYPLGHFTVLLNIG 60
           +PTLLLVSILFASFAVSLSDKFLFADSEQ KTLRFGSSVLFPVRGNVYPLGHFTVLLNIG
Sbjct: 3   IPTLLLVSILFASFAVSLSDKFLFADSEQFKTLRFGSSVLFPVRGNVYPLGHFTVLLNIG 62

Query: 61  NPSKVFELDIDTGSDLTWVQCDVECIGCTLPRDMLYRPHNNAVSREDPLCAALSSLGKFI 120
           NP KVFELDIDTGSDLTWVQCDV+CIGCTLPRDMLYRPHNNAVSREDPLCAALSSL K I
Sbjct: 63  NPPKVFELDIDTGSDLTWVQCDVQCIGCTLPRDMLYRPHNNAVSREDPLCAALSSLDKVI 122

Query: 121 FKNPNDQCAYEVEYADHGSSVGVLVKDLVPMRLTNGKRISPNLGFGCGYDQENGDLQQPP 180
           FKNPNDQC YEVEYADHGSS+G+LVKDLVPMRLTNGK I+PNLGFGCGYDQENGDLQ PP
Sbjct: 123 FKNPNDQCGYEVEYADHGSSIGILVKDLVPMRLTNGKIITPNLGFGCGYDQENGDLQPPP 182

Query: 181 SIAGVLGLSSSKATIVSQLSDLGHVSNVVGHCLTGRGEGFLFFGGDVVPSSGMSWTPILR 240
           SIAGVLGLSSSKATIVSQLSDLGHVSNVVGHCL GRG GFLFFGGD VPSS MSWTP+L 
Sbjct: 183 SIAGVLGLSSSKATIVSQLSDLGHVSNVVGHCLAGRGGGFLFFGGD-VPSSQMSWTPMLC 242

Query: 241 NSEGKYSSGPAEVYFNGRAVGIGGLTLTFDSGSSYTYFNSQVYRAIEKLLKNDLKGKPLK 300
           NSEGKYSSGPAEVYFNGRAVGIG LTLTFDSGSSYTYFNSQVYRAIEKLLKNDLKG+PLK
Sbjct: 243 NSEGKYSSGPAEVYFNGRAVGIGSLTLTFDSGSSYTYFNSQVYRAIEKLLKNDLKGQPLK 302

Query: 301 LASDDKTLELCWKGPKPFESVVDVRNFFKPLAMSFKNSKNVQFQIPPEAYLIISEFGNVC 360
           LASDDKTLELCWKGPK FESV+DVRNFFKPLAMSFKNSKNVQFQIPPEAYLIIS+FGN C
Sbjct: 303 LASDDKTLELCWKGPKAFESVIDVRNFFKPLAMSFKNSKNVQFQIPPEAYLIISKFGNAC 362

Query: 361 LGILDGSKEGMGNVNIIGDISMLNKIVVYDNERERIGWASSNCNRSPRNEASW 414
           LGILDG+KEG+GN+N+IGDISMLNKIVVYDNER++IGWAS NCNR PRNEASW
Sbjct: 363 LGILDGTKEGLGNINLIGDISMLNKIVVYDNERKQIGWASENCNRLPRNEASW 414

BLAST of CSPI04G17710 vs. ExPASy TrEMBL
Match: A0A5D3E2A0 (Aspartic proteinase Asp1-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold587G00070 PE=4 SV=1)

HSP 1 Score: 763.1 bits (1969), Expect = 6.1e-217
Identity = 373/413 (90.31%), Postives = 389/413 (94.19%), Query Frame = 0

Query: 1   MPTLLLVSILFASFAVSLSDKFLFADSEQVKTLRFGSSVLFPVRGNVYPLGHFTVLLNIG 60
           +PTLLLVSILFASFAVSLSDKFLFADSEQ KTLRFGSSVLFP+ G      HFTVLLNIG
Sbjct: 3   IPTLLLVSILFASFAVSLSDKFLFADSEQFKTLRFGSSVLFPIVG---ICRHFTVLLNIG 62

Query: 61  NPSKVFELDIDTGSDLTWVQCDVECIGCTLPRDMLYRPHNNAVSREDPLCAALSSLGKFI 120
           NP KVFELDIDTGSDLTWVQCDV+CIGCTLPRDMLYRPHNNAVSREDPLCAALSSL K I
Sbjct: 63  NPPKVFELDIDTGSDLTWVQCDVQCIGCTLPRDMLYRPHNNAVSREDPLCAALSSLDKVI 122

Query: 121 FKNPNDQCAYEVEYADHGSSVGVLVKDLVPMRLTNGKRISPNLGFGCGYDQENGDLQQPP 180
           FKNPNDQC YEVEYADHGSS+G+LVKDLVPMRLTNGK I+PNLGFGCGYDQENGDLQ PP
Sbjct: 123 FKNPNDQCGYEVEYADHGSSIGILVKDLVPMRLTNGKIITPNLGFGCGYDQENGDLQPPP 182

Query: 181 SIAGVLGLSSSKATIVSQLSDLGHVSNVVGHCLTGRGEGFLFFGGDVVPSSGMSWTPILR 240
           SIAGVLGLSSSKATIVSQLSDLGHVSNVVGHCL GRG GFLFFGGD VPSS MSWTP+L 
Sbjct: 183 SIAGVLGLSSSKATIVSQLSDLGHVSNVVGHCLAGRGGGFLFFGGD-VPSSQMSWTPMLC 242

Query: 241 NSEGKYSSGPAEVYFNGRAVGIGGLTLTFDSGSSYTYFNSQVYRAIEKLLKNDLKGKPLK 300
           NSEGKYSSGPAEVYFNGRAVGIG LTLTFDSGSSYTYFNSQVYRAIEKLLKNDLKG+PLK
Sbjct: 243 NSEGKYSSGPAEVYFNGRAVGIGSLTLTFDSGSSYTYFNSQVYRAIEKLLKNDLKGQPLK 302

Query: 301 LASDDKTLELCWKGPKPFESVVDVRNFFKPLAMSFKNSKNVQFQIPPEAYLIISEFGNVC 360
           LASDDKTLELCWKGPK FESV+DVRNFFKPLAMSFKNSKNVQFQIPPEAYLIIS+FGN C
Sbjct: 303 LASDDKTLELCWKGPKAFESVIDVRNFFKPLAMSFKNSKNVQFQIPPEAYLIISKFGNAC 362

Query: 361 LGILDGSKEGMGNVNIIGDISMLNKIVVYDNERERIGWASSNCNRSPRNEASW 414
           LGILDG+KEG+GN+N+IGDISMLNKIVVYDNER++IGWAS NCNR PRNEASW
Sbjct: 363 LGILDGTKEGLGNINLIGDISMLNKIVVYDNERKQIGWASENCNRLPRNEASW 411

BLAST of CSPI04G17710 vs. ExPASy TrEMBL
Match: A0A6J1FCF0 (aspartic proteinase Asp1-like isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111442825 PE=3 SV=1)

HSP 1 Score: 705.3 bits (1819), Expect = 1.5e-199
Identity = 337/413 (81.60%), Postives = 370/413 (89.59%), Query Frame = 0

Query: 1   MPTLLLVSILFASFAVSLSDKFLFADSEQVKTLRFGSSVLFPVRGNVYPLGHFTVLLNIG 60
           MP LL VSILFA FAVSLSD+FL AD EQ++++ FGSS+LFPV+GNVYPLGHFTV + IG
Sbjct: 17  MPVLLFVSILFAIFAVSLSDQFLTADWEQLRSVGFGSSILFPVKGNVYPLGHFTVSVTIG 76

Query: 61  NPSKVFELDIDTGSDLTWVQCDVECIGCTLPRDMLYRPHNNAVSREDPLCAALSSLGKFI 120
           NP K FELDIDTGSDLTWVQCDV+C GCTLPRDMLY+PHNNAVSREDPLCAALSS G   
Sbjct: 77  NPPKAFELDIDTGSDLTWVQCDVQCTGCTLPRDMLYKPHNNAVSREDPLCAALSSPGNAP 136

Query: 121 FKNPNDQCAYEVEYADHGSSVGVLVKDLVPMRLTNGKRISPNLGFGCGYDQENGDLQQPP 180
            K  NDQC Y+VEYADHGSS+GVLVKDL PMRLTNG+ I+PNLGFGCGYDQ+ G +Q PP
Sbjct: 137 CKKSNDQCDYDVEYADHGSSIGVLVKDLAPMRLTNGQLINPNLGFGCGYDQDYGGMQPPP 196

Query: 181 SIAGVLGLSSSKATIVSQLSDLGHVSNVVGHCLTGRGEGFLFFGGDVVPSSGMSWTPILR 240
            +AGVLGLSSSKATIVSQL+DLGHVSNV+GHCL G G GFLFFGG +VPSSGMSWTPILR
Sbjct: 197 LVAGVLGLSSSKATIVSQLNDLGHVSNVIGHCLAGLGGGFLFFGGGLVPSSGMSWTPILR 256

Query: 241 NSEGKYSSGPAEVYFNGRAVGIGGLTLTFDSGSSYTYFNSQVYRAIEKLLKNDLKGKPLK 300
           NSEGKYSSGPAEVYF G+A GI GLTLTFDSGSSYTYFNSQVY A+EKLLKNDLKG+PLK
Sbjct: 257 NSEGKYSSGPAEVYFGGKAGGIKGLTLTFDSGSSYTYFNSQVYGAVEKLLKNDLKGQPLK 316

Query: 301 LASDDKTLELCWKGPKPFESVVDVRNFFKPLAMSFKNSKNVQFQIPPEAYLIISEFGNVC 360
           LA DDKTLELCWKG K F+SV DVR+FFKPLA++FKNSKN QFQIPPEAYLIISEFGNVC
Sbjct: 317 LAPDDKTLELCWKGRKAFKSVSDVRDFFKPLALNFKNSKNAQFQIPPEAYLIISEFGNVC 376

Query: 361 LGILDGSKEGMGNVNIIGDISMLNKIVVYDNERERIGWASSNCNRSPRNEASW 414
           LGILDG+KEG+GNVN+IGDISMLNKIVVYDN+R +IGWA  NCN  PRNEAS+
Sbjct: 377 LGILDGTKEGLGNVNLIGDISMLNKIVVYDNQRRQIGWAPQNCNPPPRNEASY 429

BLAST of CSPI04G17710 vs. ExPASy TrEMBL
Match: A0A6J1IHP1 (aspartic proteinase Asp1-like isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111477022 PE=4 SV=1)

HSP 1 Score: 704.5 bits (1817), Expect = 2.6e-199
Identity = 338/413 (81.84%), Postives = 368/413 (89.10%), Query Frame = 0

Query: 1   MPTLLLVSILFASFAVSLSDKFLFADSEQVKTLRFGSSVLFPVRGNVYPLGHFTVLLNIG 60
           MP LL VSILFA FAVSLSDKFL AD EQ++++ FGSS+LFPV+GNVYPLGHFTV + IG
Sbjct: 17  MPVLLFVSILFAIFAVSLSDKFLTADWEQLRSVGFGSSILFPVKGNVYPLGHFTVSVKIG 76

Query: 61  NPSKVFELDIDTGSDLTWVQCDVECIGCTLPRDMLYRPHNNAVSREDPLCAALSSLGKFI 120
            P K FELDIDTGSDLTWVQCDV+CIGCTLPRDMLY+PHNNAVSREDPLCAALSS G   
Sbjct: 77  KPPKAFELDIDTGSDLTWVQCDVQCIGCTLPRDMLYKPHNNAVSREDPLCAALSSPGNAP 136

Query: 121 FKNPNDQCAYEVEYADHGSSVGVLVKDLVPMRLTNGKRISPNLGFGCGYDQENGDLQQPP 180
            K  NDQC YEVEYADHGSS+GVLVKDL PMRLTNG+ I+PNLGFGCGYDQ++G  Q PP
Sbjct: 137 CKKSNDQCVYEVEYADHGSSIGVLVKDLAPMRLTNGQLINPNLGFGCGYDQDDGGTQPPP 196

Query: 181 SIAGVLGLSSSKATIVSQLSDLGHVSNVVGHCLTGRGEGFLFFGGDVVPSSGMSWTPILR 240
           S+AGVLGLS SKATIVSQLSDLGHVSNV+GHCL   G GFLFFG  +VPSSGMSWTPILR
Sbjct: 197 SVAGVLGLSCSKATIVSQLSDLGHVSNVIGHCLAELGGGFLFFGAGLVPSSGMSWTPILR 256

Query: 241 NSEGKYSSGPAEVYFNGRAVGIGGLTLTFDSGSSYTYFNSQVYRAIEKLLKNDLKGKPLK 300
           NSEGKYSSGPAEVYF G+A GI GLTLTFDSGSSYTYFNSQVY A+EKLLKNDLKG+PLK
Sbjct: 257 NSEGKYSSGPAEVYFGGKAGGIRGLTLTFDSGSSYTYFNSQVYGAVEKLLKNDLKGQPLK 316

Query: 301 LASDDKTLELCWKGPKPFESVVDVRNFFKPLAMSFKNSKNVQFQIPPEAYLIISEFGNVC 360
           LA DDKTLELCWKG K F+SV DVR+FFKPLA++FKNSKN QFQIPPEAYLIISEFGNVC
Sbjct: 317 LAPDDKTLELCWKGRKAFKSVSDVRDFFKPLALNFKNSKNAQFQIPPEAYLIISEFGNVC 376

Query: 361 LGILDGSKEGMGNVNIIGDISMLNKIVVYDNERERIGWASSNCNRSPRNEASW 414
           LGILDG+KEG+GNVN+IGDISMLNKIVVYDN+R +IGWA  NCN  PRNEAS+
Sbjct: 377 LGILDGTKEGLGNVNLIGDISMLNKIVVYDNQRRQIGWAPQNCNPPPRNEASY 429

BLAST of CSPI04G17710 vs. NCBI nr
Match: XP_004142630.1 (aspartic proteinase Asp1 [Cucumis sativus] >KGN54552.1 hypothetical protein Csa_012029 [Cucumis sativus])

HSP 1 Score: 842.8 bits (2176), Expect = 1.3e-240
Identity = 411/413 (99.52%), Postives = 411/413 (99.52%), Query Frame = 0

Query: 1   MPTLLLVSILFASFAVSLSDKFLFADSEQVKTLRFGSSVLFPVRGNVYPLGHFTVLLNIG 60
           MPTLLLVSILFASFAVSLSDKFLFADSEQVKTLRFGSSVLFPVRGNVYPLGHFTVLLNIG
Sbjct: 1   MPTLLLVSILFASFAVSLSDKFLFADSEQVKTLRFGSSVLFPVRGNVYPLGHFTVLLNIG 60

Query: 61  NPSKVFELDIDTGSDLTWVQCDVECIGCTLPRDMLYRPHNNAVSREDPLCAALSSLGKFI 120
           NPSKVFELDIDTGSDLTWVQCDVECIGCTLPRDMLYRPHNNAVSREDPLCAALSSLGKFI
Sbjct: 61  NPSKVFELDIDTGSDLTWVQCDVECIGCTLPRDMLYRPHNNAVSREDPLCAALSSLGKFI 120

Query: 121 FKNPNDQCAYEVEYADHGSSVGVLVKDLVPMRLTNGKRISPNLGFGCGYDQENGDLQQPP 180
           FKNPNDQCAYEVEYADHGSSVGVLVKDLVPMRLTNGKRISPNLGFGCGYDQENGDLQQPP
Sbjct: 121 FKNPNDQCAYEVEYADHGSSVGVLVKDLVPMRLTNGKRISPNLGFGCGYDQENGDLQQPP 180

Query: 181 SIAGVLGLSSSKATIVSQLSDLGHVSNVVGHCLTGRGEGFLFFGGDVVPSSGMSWTPILR 240
           SIAGVLGLSSSKATIVSQLSDLGHVSNVVGHCLTGRG GFLFFGGDVVPSSGMSWTPILR
Sbjct: 181 SIAGVLGLSSSKATIVSQLSDLGHVSNVVGHCLTGRGGGFLFFGGDVVPSSGMSWTPILR 240

Query: 241 NSEGKYSSGPAEVYFNGRAVGIGGLTLTFDSGSSYTYFNSQVYRAIEKLLKNDLKGKPLK 300
           NSEGKYSSGPAEVYFNGRAVGIGGLTLTFDSGSSYTYFNSQVYRAIEKLLKNDLKG PLK
Sbjct: 241 NSEGKYSSGPAEVYFNGRAVGIGGLTLTFDSGSSYTYFNSQVYRAIEKLLKNDLKGNPLK 300

Query: 301 LASDDKTLELCWKGPKPFESVVDVRNFFKPLAMSFKNSKNVQFQIPPEAYLIISEFGNVC 360
           LASDDKTLELCWKGPKPFESVVDVRNFFKPLAMSFKNSKNVQFQIPPEAYLIISEFGNVC
Sbjct: 301 LASDDKTLELCWKGPKPFESVVDVRNFFKPLAMSFKNSKNVQFQIPPEAYLIISEFGNVC 360

Query: 361 LGILDGSKEGMGNVNIIGDISMLNKIVVYDNERERIGWASSNCNRSPRNEASW 414
           LGILDGSKEGMGNVNIIGDISMLNKIVVYDNERERIGWASSNCNRSPRNEASW
Sbjct: 361 LGILDGSKEGMGNVNIIGDISMLNKIVVYDNERERIGWASSNCNRSPRNEASW 413

BLAST of CSPI04G17710 vs. NCBI nr
Match: XP_008444184.1 (PREDICTED: aspartic proteinase Asp1-like [Cucumis melo])

HSP 1 Score: 787.3 bits (2032), Expect = 6.3e-224
Identity = 381/413 (92.25%), Postives = 396/413 (95.88%), Query Frame = 0

Query: 1   MPTLLLVSILFASFAVSLSDKFLFADSEQVKTLRFGSSVLFPVRGNVYPLGHFTVLLNIG 60
           +PTLLLVSILFASFAVSLSDKFLFADSEQ KTLRFGSSVLFPVRGNVYPLGHFTVLLNIG
Sbjct: 3   IPTLLLVSILFASFAVSLSDKFLFADSEQFKTLRFGSSVLFPVRGNVYPLGHFTVLLNIG 62

Query: 61  NPSKVFELDIDTGSDLTWVQCDVECIGCTLPRDMLYRPHNNAVSREDPLCAALSSLGKFI 120
           NP KVFELDIDTGSDLTWVQCDV+CIGCTLPRDMLYRPHNNAVSREDPLCAALSSL K I
Sbjct: 63  NPPKVFELDIDTGSDLTWVQCDVQCIGCTLPRDMLYRPHNNAVSREDPLCAALSSLDKVI 122

Query: 121 FKNPNDQCAYEVEYADHGSSVGVLVKDLVPMRLTNGKRISPNLGFGCGYDQENGDLQQPP 180
           FKNPNDQC YEVEYADHGSS+G+LVKDLVPMRLTNGK I+PNLGFGCGYDQENGDLQ PP
Sbjct: 123 FKNPNDQCGYEVEYADHGSSIGILVKDLVPMRLTNGKIITPNLGFGCGYDQENGDLQPPP 182

Query: 181 SIAGVLGLSSSKATIVSQLSDLGHVSNVVGHCLTGRGEGFLFFGGDVVPSSGMSWTPILR 240
           SIAGVLGLSSSKATIVSQLSDLGHVSNVVGHCL GRG GFLFFGGD VPSS MSWTP+L 
Sbjct: 183 SIAGVLGLSSSKATIVSQLSDLGHVSNVVGHCLAGRGGGFLFFGGD-VPSSQMSWTPMLC 242

Query: 241 NSEGKYSSGPAEVYFNGRAVGIGGLTLTFDSGSSYTYFNSQVYRAIEKLLKNDLKGKPLK 300
           NSEGKYSSGPAEVYFNGRAVGIG LTLTFDSGSSYTYFNSQVYRAIEKLLKNDLKG+PLK
Sbjct: 243 NSEGKYSSGPAEVYFNGRAVGIGSLTLTFDSGSSYTYFNSQVYRAIEKLLKNDLKGQPLK 302

Query: 301 LASDDKTLELCWKGPKPFESVVDVRNFFKPLAMSFKNSKNVQFQIPPEAYLIISEFGNVC 360
           LASDDKTLELCWKGPK FESV+DVRNFFKPLAMSFKNSKNVQFQIPPEAYLIIS+FGN C
Sbjct: 303 LASDDKTLELCWKGPKAFESVIDVRNFFKPLAMSFKNSKNVQFQIPPEAYLIISKFGNAC 362

Query: 361 LGILDGSKEGMGNVNIIGDISMLNKIVVYDNERERIGWASSNCNRSPRNEASW 414
           LGILDG+KEG+GN+N+IGDISMLNKIVVYDNER++IGWAS NCNR PRNEASW
Sbjct: 363 LGILDGTKEGLGNINLIGDISMLNKIVVYDNERKQIGWASENCNRLPRNEASW 414

BLAST of CSPI04G17710 vs. NCBI nr
Match: TYK30006.1 (aspartic proteinase Asp1-like [Cucumis melo var. makuwa])

HSP 1 Score: 763.1 bits (1969), Expect = 1.3e-216
Identity = 373/413 (90.31%), Postives = 389/413 (94.19%), Query Frame = 0

Query: 1   MPTLLLVSILFASFAVSLSDKFLFADSEQVKTLRFGSSVLFPVRGNVYPLGHFTVLLNIG 60
           +PTLLLVSILFASFAVSLSDKFLFADSEQ KTLRFGSSVLFP+ G      HFTVLLNIG
Sbjct: 3   IPTLLLVSILFASFAVSLSDKFLFADSEQFKTLRFGSSVLFPIVG---ICRHFTVLLNIG 62

Query: 61  NPSKVFELDIDTGSDLTWVQCDVECIGCTLPRDMLYRPHNNAVSREDPLCAALSSLGKFI 120
           NP KVFELDIDTGSDLTWVQCDV+CIGCTLPRDMLYRPHNNAVSREDPLCAALSSL K I
Sbjct: 63  NPPKVFELDIDTGSDLTWVQCDVQCIGCTLPRDMLYRPHNNAVSREDPLCAALSSLDKVI 122

Query: 121 FKNPNDQCAYEVEYADHGSSVGVLVKDLVPMRLTNGKRISPNLGFGCGYDQENGDLQQPP 180
           FKNPNDQC YEVEYADHGSS+G+LVKDLVPMRLTNGK I+PNLGFGCGYDQENGDLQ PP
Sbjct: 123 FKNPNDQCGYEVEYADHGSSIGILVKDLVPMRLTNGKIITPNLGFGCGYDQENGDLQPPP 182

Query: 181 SIAGVLGLSSSKATIVSQLSDLGHVSNVVGHCLTGRGEGFLFFGGDVVPSSGMSWTPILR 240
           SIAGVLGLSSSKATIVSQLSDLGHVSNVVGHCL GRG GFLFFGGD VPSS MSWTP+L 
Sbjct: 183 SIAGVLGLSSSKATIVSQLSDLGHVSNVVGHCLAGRGGGFLFFGGD-VPSSQMSWTPMLC 242

Query: 241 NSEGKYSSGPAEVYFNGRAVGIGGLTLTFDSGSSYTYFNSQVYRAIEKLLKNDLKGKPLK 300
           NSEGKYSSGPAEVYFNGRAVGIG LTLTFDSGSSYTYFNSQVYRAIEKLLKNDLKG+PLK
Sbjct: 243 NSEGKYSSGPAEVYFNGRAVGIGSLTLTFDSGSSYTYFNSQVYRAIEKLLKNDLKGQPLK 302

Query: 301 LASDDKTLELCWKGPKPFESVVDVRNFFKPLAMSFKNSKNVQFQIPPEAYLIISEFGNVC 360
           LASDDKTLELCWKGPK FESV+DVRNFFKPLAMSFKNSKNVQFQIPPEAYLIIS+FGN C
Sbjct: 303 LASDDKTLELCWKGPKAFESVIDVRNFFKPLAMSFKNSKNVQFQIPPEAYLIISKFGNAC 362

Query: 361 LGILDGSKEGMGNVNIIGDISMLNKIVVYDNERERIGWASSNCNRSPRNEASW 414
           LGILDG+KEG+GN+N+IGDISMLNKIVVYDNER++IGWAS NCNR PRNEASW
Sbjct: 363 LGILDGTKEGLGNINLIGDISMLNKIVVYDNERKQIGWASENCNRLPRNEASW 411

BLAST of CSPI04G17710 vs. NCBI nr
Match: XP_038896620.1 (aspartic proteinase Asp1-like [Benincasa hispida])

HSP 1 Score: 743.8 bits (1919), Expect = 8.0e-211
Identity = 354/412 (85.92%), Postives = 383/412 (92.96%), Query Frame = 0

Query: 2   PTLLLVSILFASFAVSLSDKFLFADSEQVKTLRFGSSVLFPVRGNVYPLGHFTVLLNIGN 61
           P LLL+SILFA+FAVSLSDKFL ADSE +KTLRFGSS+LFPV+GNVYPLGHFTVLLNIGN
Sbjct: 222 PVLLLLSILFATFAVSLSDKFLIADSELLKTLRFGSSILFPVKGNVYPLGHFTVLLNIGN 281

Query: 62  PSKVFELDIDTGSDLTWVQCDVECIGCTLPRDMLYRPHNNAVSREDPLCAALSSLGKFIF 121
           P KVFELDIDTGSDLTWVQCDV+CIGCTLPRDMLYRPHNNAV+REDPLC ALSS GK + 
Sbjct: 282 PPKVFELDIDTGSDLTWVQCDVQCIGCTLPRDMLYRPHNNAVNREDPLCVALSSPGKALC 341

Query: 122 KNPNDQCAYEVEYADHGSSVGVLVKDLVPMRLTNGKRISPNLGFGCGYDQENGDLQQPPS 181
           KNPNDQC YEVEYADHGSS+GVLVKDLVPMRLTNGK I+PNLGFGCGYDQ+NGDLQ PPS
Sbjct: 342 KNPNDQCGYEVEYADHGSSIGVLVKDLVPMRLTNGKLITPNLGFGCGYDQDNGDLQPPPS 401

Query: 182 IAGVLGLSSSKATIVSQLSDLGHVSNVVGHCLTGRGEGFLFFGGDVVPSSGMSWTPILRN 241
           I GVLGLSSSKATIVSQLSDLG VSNV+GHCL GRG GFL+ G D VPSS MSWTP+L +
Sbjct: 402 IGGVLGLSSSKATIVSQLSDLGLVSNVIGHCLAGRGGGFLYLGEDFVPSSPMSWTPMLHD 461

Query: 242 SEGKYSSGPAEVYFNGRAVGIGGLTLTFDSGSSYTYFNSQVYRAIEKLLKNDLKGKPLKL 301
           SEGKYSSGPAEVYF GRAVGIGGLTLTFDSGSSYTYFNSQVY A+EKLL+NDLKG+PLKL
Sbjct: 462 SEGKYSSGPAEVYFGGRAVGIGGLTLTFDSGSSYTYFNSQVYGAVEKLLRNDLKGQPLKL 521

Query: 302 ASDDKTLELCWKGPKPFESVVDVRNFFKPLAMSFKNSKNVQFQIPPEAYLIISEFGNVCL 361
           A DDKTLELCWKGPK F+S+VDVRNFFKPLA+SFKN KNVQF IPPEAYLIIS+ GNVCL
Sbjct: 522 APDDKTLELCWKGPKVFKSLVDVRNFFKPLALSFKNFKNVQFHIPPEAYLIISKSGNVCL 581

Query: 362 GILDGSKEGMGNVNIIGDISMLNKIVVYDNERERIGWASSNCNRSPRNEASW 414
           GILDG+KEG+GNVN+IGDISMLNKIVVYDN+R++IGWAS NCNR P+NEASW
Sbjct: 582 GILDGTKEGLGNVNLIGDISMLNKIVVYDNQRQQIGWASENCNRPPKNEASW 633

BLAST of CSPI04G17710 vs. NCBI nr
Match: KAG6591879.1 (Aspartic proteinase Asp1, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 714.9 bits (1844), Expect = 4.0e-202
Identity = 341/413 (82.57%), Postives = 373/413 (90.31%), Query Frame = 0

Query: 1   MPTLLLVSILFASFAVSLSDKFLFADSEQVKTLRFGSSVLFPVRGNVYPLGHFTVLLNIG 60
           MP LL VSILFA FAVSLSDKFL AD EQ++++ FG S+LFPV+GNVYPLGHFTV + IG
Sbjct: 17  MPVLLFVSILFAIFAVSLSDKFLTADWEQLRSVGFGPSILFPVKGNVYPLGHFTVSVKIG 76

Query: 61  NPSKVFELDIDTGSDLTWVQCDVECIGCTLPRDMLYRPHNNAVSREDPLCAALSSLGKFI 120
           NP K FELDIDTGSDLTWVQCDV+CIGCTLPRDMLY+PHNNAVSREDPLCAALSS G   
Sbjct: 77  NPPKAFELDIDTGSDLTWVQCDVQCIGCTLPRDMLYKPHNNAVSREDPLCAALSSPGNAP 136

Query: 121 FKNPNDQCAYEVEYADHGSSVGVLVKDLVPMRLTNGKRISPNLGFGCGYDQENGDLQQPP 180
            K  NDQC YEVEYADHGSS+GVLVKDL PMRLTNG+ I+PNLGFGCGYDQ++G +Q PP
Sbjct: 137 CKKSNDQCDYEVEYADHGSSIGVLVKDLAPMRLTNGQLINPNLGFGCGYDQDDGGMQPPP 196

Query: 181 SIAGVLGLSSSKATIVSQLSDLGHVSNVVGHCLTGRGEGFLFFGGDVVPSSGMSWTPILR 240
           S+AGVLGLSSSKATIVSQLSDLGHVSNV+GHCL G G GFLFFGG +VPSSGMSWTPILR
Sbjct: 197 SVAGVLGLSSSKATIVSQLSDLGHVSNVIGHCLAGLGGGFLFFGGGLVPSSGMSWTPILR 256

Query: 241 NSEGKYSSGPAEVYFNGRAVGIGGLTLTFDSGSSYTYFNSQVYRAIEKLLKNDLKGKPLK 300
           NSEGKYSSGPAEVYF G+A GI GLTLTFDSGSSYTYFNSQVY A+EKLLKNDLKG+PLK
Sbjct: 257 NSEGKYSSGPAEVYFGGKAGGIRGLTLTFDSGSSYTYFNSQVYGAVEKLLKNDLKGQPLK 316

Query: 301 LASDDKTLELCWKGPKPFESVVDVRNFFKPLAMSFKNSKNVQFQIPPEAYLIISEFGNVC 360
           LA DDKTLELCWKGPK F+SV DVR+FFKPLA++FKNSKN QFQIPPEAYLIISEFGNVC
Sbjct: 317 LAPDDKTLELCWKGPKAFKSVSDVRDFFKPLALNFKNSKNAQFQIPPEAYLIISEFGNVC 376

Query: 361 LGILDGSKEGMGNVNIIGDISMLNKIVVYDNERERIGWASSNCNRSPRNEASW 414
           LGILDG+KEG+GNVN+IGDISMLNKIVVYDN+R +IGWA  NCN  PRNEA++
Sbjct: 377 LGILDGTKEGLGNVNLIGDISMLNKIVVYDNQRRQIGWAPQNCNPPPRNEAAY 429

BLAST of CSPI04G17710 vs. TAIR 10
Match: AT1G44130.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 444.9 bits (1143), Expect = 7.1e-125
Identity = 199/373 (53.35%), Postives = 273/373 (73.19%), Query Frame = 0

Query: 37  SSVLFPVRGNVYPLGHFTVLLNIGNPSKVFELDIDTGSDLTWVQCDVECIGCTLPRDMLY 96
           SSV+FP+ GNV+PLG+++VL+ IG+P K F+ DIDTGSDLTWVQCD  C GCTLP ++ Y
Sbjct: 33  SSVVFPLSGNVFPLGYYSVLMQIGSPPKAFQFDIDTGSDLTWVQCDAPCSGCTLPPNLQY 92

Query: 97  RPHNNAVSREDPLCAALSSLGKFIFKNPNDQCAYEVEYADHGSSVGVLVKDLVPMRLTNG 156
           +P  N +   +P+C AL    K    NP +QC YEV+YAD GSS+G LV D  P++L NG
Sbjct: 93  KPKGNIIPCSNPICTALHWPNKPHCPNPQEQCDYEVKYADQGSSMGALVTDQFPLKLVNG 152

Query: 157 KRISPNLGFGCGYDQENGDLQQPPSIAGVLGLSSSKATIVSQLSDLGHVSNVVGHCLTGR 216
             + P + FGCGYDQ       PP+ AGVLGL   K  +++QL   G   NVVGHCL+ +
Sbjct: 153 SFMQPPVAFGCGYDQSYPSAHPPPATAGVLGLGRGKIGLLTQLVSAGLTRNVVGHCLSSK 212

Query: 217 GEGFLFFGGDVVPSSGMSWTPILRNSEGKYSSGPAEVYFNGRAVGIGGLTLTFDSGSSYT 276
           G GFLFFG ++VPS G++WTP+L + +  Y++GPA++ FNG+  G+ GL L FD+GSSYT
Sbjct: 213 GGGFLFFGDNLVPSIGVAWTPLL-SQDNHYTTGPADLLFNGKPTGLKGLKLIFDTGSSYT 272

Query: 277 YFNSQVYRAIEKLLKNDLKGKPLKLASDDKTLELCWKGPKPFESVVDVRNFFKPLAMSFK 336
           YFNS+ Y+ I  L+ NDLK  PLK+A +DKTL +CWKG KPF+SV++V+NFFK + ++F 
Sbjct: 273 YFNSKAYQTIINLIGNDLKVSPLKVAKEDKTLPICWKGAKPFKSVLEVKNFFKTITINFT 332

Query: 337 NS-KNVQFQIPPEAYLIISEFGNVCLGILDGSKEGMGNVNIIGDISMLNKIVVYDNERER 396
           N  +N Q  + PE YLI+S+ GNVCLG+L+GS+ G+ N N+IGDISM   +++YDNE+++
Sbjct: 333 NGRRNTQLYLAPELYLIVSKTGNVCLGLLNGSEVGLQNSNVIGDISMQGLMMIYDNEKQQ 392

Query: 397 IGWASSNCNRSPR 409
           +GW SS+CN+ P+
Sbjct: 393 LGWVSSDCNKLPK 404

BLAST of CSPI04G17710 vs. TAIR 10
Match: AT1G77480.2 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 433.7 bits (1114), Expect = 1.6e-121
Identity = 211/408 (51.72%), Postives = 279/408 (68.38%), Query Frame = 0

Query: 7   VSILFASFAVSLSDKFLFADSEQVK--TLRFGSSVLFPVRGNVYPLGHFTVLLNIGNPSK 66
           V IL A F  S + K     S QVK    R  S+V+FPV GNVYPLG++ VLLNIGNP K
Sbjct: 22  VFILCARFQTSEATK---DSSAQVKLQNRRLSSTVVFPVSGNVYPLGYYYVLLNIGNPPK 81

Query: 67  VFELDIDTGSDLTWVQCDVECIGCTLPRDMLYRPHNNAVSREDPLCAALSSLGKFIFKNP 126
           +F+LDIDTGSDLTWVQCD  C GCT PR   Y+P++N +     LC+ L         +P
Sbjct: 82  LFDLDIDTGSDLTWVQCDAPCNGCTKPRAKQYKPNHNTLPCSHILCSGLDLPQDRPCADP 141

Query: 127 NDQCAYEVEYADHGSSVGVLVKDLVPMRLTNGKRISPNLGFGCGYDQENGDLQQPPSIAG 186
            DQC YE+ Y+DH SS+G LV D VP++L NG  ++  L FGCGYDQ+N     PP  AG
Sbjct: 142 EDQCDYEIGYSDHASSIGALVTDEVPLKLANGSIMNLRLTFGCGYDQQNPGPHPPPPTAG 201

Query: 187 VLGLSSSKATIVSQLSDLGHVSNVVGHCLTGRGEGFLFFGGDVVPSSGMSWTPILRNSEG 246
           +LGL   K  + +QL  LG   NV+ HCL+  G+GFL  G ++VPSSG++WT +  NS  
Sbjct: 202 ILGLGRGKVGLSTQLKSLGITKNVIVHCLSHTGKGFLSIGDELVPSSGVTWTSLATNSPS 261

Query: 247 K-YSSGPAEVYFNGRAVGIGGLTLTFDSGSSYTYFNSQVYRAIEKLLKNDLKGKPLKLAS 306
           K Y +GPAE+ FN +  G+ G+ + FDSGSSYTYFN++ Y+AI  L++ DL GKPL    
Sbjct: 262 KNYMAGPAELLFNDKTTGVKGINVVFDSGSSYTYFNAEAYQAILDLIRKDLNGKPLTDTK 321

Query: 307 DDKTLELCWKGPKPFESVVDVRNFFKPLAMSFKNSKNVQ-FQIPPEAYLIISEFGNVCLG 366
           DDK+L +CWKG KP +S+ +V+ +FK + + F N KN Q FQ+PPE+YLII+E G VCLG
Sbjct: 322 DDKSLPVCWKGKKPLKSLDEVKKYFKTITLRFGNQKNGQLFQVPPESYLIITEKGRVCLG 381

Query: 367 ILDGSKEGMGNVNIIGDISMLNKIVVYDNERERIGWASSNCNRSPRNE 411
           IL+G++ G+   NIIGDIS    +V+YDNE++RIGW SS+C++ P++E
Sbjct: 382 ILNGTEIGLEGYNIIGDISFQGIMVIYDNEKQRIGWISSDCDKLPKSE 426

BLAST of CSPI04G17710 vs. TAIR 10
Match: AT1G77480.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 431.0 bits (1107), Expect = 1.1e-120
Identity = 210/405 (51.85%), Postives = 276/405 (68.15%), Query Frame = 0

Query: 7   VSILFASFAVSLSDKFLFADSEQVK--TLRFGSSVLFPVRGNVYPLGHFTVLLNIGNPSK 66
           V IL A F  S + K     S QVK    R  S+V+FPV GNVYPLG++ VLLNIGNP K
Sbjct: 22  VFILCARFQTSEATK---DSSAQVKLQNRRLSSTVVFPVSGNVYPLGYYYVLLNIGNPPK 81

Query: 67  VFELDIDTGSDLTWVQCDVECIGCTLPRDMLYRPHNNAVSREDPLCAALSSLGKFIFKNP 126
           +F+LDIDTGSDLTWVQCD  C GCT PR   Y+P++N +     LC+ L         +P
Sbjct: 82  LFDLDIDTGSDLTWVQCDAPCNGCTKPRAKQYKPNHNTLPCSHILCSGLDLPQDRPCADP 141

Query: 127 NDQCAYEVEYADHGSSVGVLVKDLVPMRLTNGKRISPNLGFGCGYDQENGDLQQPPSIAG 186
            DQC YE+ Y+DH SS+G LV D VP++L NG  ++  L FGCGYDQ+N     PP  AG
Sbjct: 142 EDQCDYEIGYSDHASSIGALVTDEVPLKLANGSIMNLRLTFGCGYDQQNPGPHPPPPTAG 201

Query: 187 VLGLSSSKATIVSQLSDLGHVSNVVGHCLTGRGEGFLFFGGDVVPSSGMSWTPILRNSEG 246
           +LGL   K  + +QL  LG   NV+ HCL+  G+GFL  G ++VPSSG++WT +  NS  
Sbjct: 202 ILGLGRGKVGLSTQLKSLGITKNVIVHCLSHTGKGFLSIGDELVPSSGVTWTSLATNSPS 261

Query: 247 K-YSSGPAEVYFNGRAVGIGGLTLTFDSGSSYTYFNSQVYRAIEKLLKNDLKGKPLKLAS 306
           K Y +GPAE+ FN +  G+ G+ + FDSGSSYTYFN++ Y+AI  L++ DL GKPL    
Sbjct: 262 KNYMAGPAELLFNDKTTGVKGINVVFDSGSSYTYFNAEAYQAILDLIRKDLNGKPLTDTK 321

Query: 307 DDKTLELCWKGPKPFESVVDVRNFFKPLAMSFKNSKNVQ-FQIPPEAYLIISEFGNVCLG 366
           DDK+L +CWKG KP +S+ +V+ +FK + + F N KN Q FQ+PPE+YLII+E G VCLG
Sbjct: 322 DDKSLPVCWKGKKPLKSLDEVKKYFKTITLRFGNQKNGQLFQVPPESYLIITEKGRVCLG 381

Query: 367 ILDGSKEGMGNVNIIGDISMLNKIVVYDNERERIGWASSNCNRSP 408
           IL+G++ G+   NIIGDIS    +V+YDNE++RIGW SS+C++ P
Sbjct: 382 ILNGTEIGLEGYNIIGDISFQGIMVIYDNEKQRIGWISSDCDKLP 423

BLAST of CSPI04G17710 vs. TAIR 10
Match: AT4G33490.2 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 400.6 bits (1028), Expect = 1.5e-111
Identity = 200/404 (49.50%), Postives = 266/404 (65.84%), Query Frame = 0

Query: 4   LLLVSILFASFAVSLSDKFLFADSEQVKTLRFGSSVLFPVRGNVYPLGHFTVLLNIGNPS 63
           +L+V  L   F+ ++  ++        +  R  SSV+FPV GNVYPLG++ V +NIG P 
Sbjct: 11  VLMVMSLVLGFSSAVDFRWRKTAGFSDRFTRAVSSVVFPVHGNVYPLGYYNVTINIGQPP 70

Query: 64  KVFELDIDTGSDLTWVQCDVECIGCTLPRDMLYRPHNNAVSREDPLCAALSSLGKFIFKN 123
           + + LD+DTGSDLTW+QCD  C+ C      LY+P ++ +   DPLC AL        + 
Sbjct: 71  RPYYLDLDTGSDLTWLQCDAPCVRCLEAPHPLYQPSSDLIPCNDPLCKALHLNSNQRCET 130

Query: 124 PNDQCAYEVEYADHGSSVGVLVKDLVPMRLTNGKRISPNLGFGCGYDQENGDLQQPPSIA 183
           P +QC YEVEYAD GSS+GVLV+D+  M  T G R++P L  GCGYDQ  G     P + 
Sbjct: 131 P-EQCDYEVEYADGGSSLGVLVRDVFSMNYTQGLRLTPRLALGCGYDQIPGASSHHP-LD 190

Query: 184 GVLGLSSSKATIVSQLSDLGHVSNVVGHCLTGRGEGFLFFGGDVVPSSGMSWTPILRNSE 243
           GVLGL   K +I+SQL   G+V NV+GHCL+  G G LFFG D+  SS +SWTP+ R   
Sbjct: 191 GVLGLGRGKVSILSQLHSQGYVKNVIGHCLSSLGGGILFFGDDLYDSSRVSWTPMSREYS 250

Query: 244 GKYSSG-PAEVYFNGRAVGIGGLTLTFDSGSSYTYFNSQVYRAIEKLLKNDLKGKPLKLA 303
             YS     E+ F GR  G+  L   FDSGSSYTYFNS+ Y+A+  LLK +L GKPLK A
Sbjct: 251 KHYSPAMGGELLFGGRTTGLKNLLTVFDSGSSYTYFNSKAYQAVTYLLKRELSGKPLKEA 310

Query: 304 SDDKTLELCWKGPKPFESVVDVRNFFKPLAMSFKNS--KNVQFQIPPEAYLIISEFGNVC 363
            DD TL LCW+G +PF S+ +V+ +FKPLA+SFK        F+IPPEAYLIIS  GNVC
Sbjct: 311 RDDHTLPLCWQGRRPFMSIEEVKKYFKPLALSFKTGWRSKTLFEIPPEAYLIISMKGNVC 370

Query: 364 LGILDGSKEGMGNVNIIGDISMLNKIVVYDNERERIGWASSNCN 405
           LGIL+G++ G+ N+N+IGDISM +++++YDNE++ IGW   +C+
Sbjct: 371 LGILNGTEIGLQNLNLIGDISMQDQMIIYDNEKQSIGWMPVDCD 412

BLAST of CSPI04G17710 vs. TAIR 10
Match: AT4G33490.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 369.0 bits (946), Expect = 5.0e-102
Identity = 188/378 (49.74%), Postives = 245/378 (64.81%), Query Frame = 0

Query: 4   LLLVSILFASFAVSLSDKFLFADSEQVKTLRFGSSVLFPVRGNVYPLGHFTVLLNIGNPS 63
           +L+V  L   F+ ++  ++        +  R  SSV+FPV GNVYPLG++ V +NIG P 
Sbjct: 8   VLMVMSLVLGFSSAVDFRWRKTAGFSDRFTRAVSSVVFPVHGNVYPLGYYNVTINIGQPP 67

Query: 64  KVFELDIDTGSDLTWVQCDVECIGCTLPRDMLYRPHNNAVSREDPLCAALSSLGKFIFKN 123
           + + LD+DTGSDLTW+QCD  C+ C      LY+P ++ +   DPLC AL        + 
Sbjct: 68  RPYYLDLDTGSDLTWLQCDAPCVRCLEAPHPLYQPSSDLIPCNDPLCKALHLNSNQRCET 127

Query: 124 PNDQCAYEVEYADHGSSVGVLVKDLVPMRLTNGKRISPNLGFGCGYDQENGDLQQPPSIA 183
           P +QC YEVEYAD GSS+GVLV+D+  M  T G R++P L  GCGYDQ  G     P + 
Sbjct: 128 P-EQCDYEVEYADGGSSLGVLVRDVFSMNYTQGLRLTPRLALGCGYDQIPGASSHHP-LD 187

Query: 184 GVLGLSSSKATIVSQLSDLGHVSNVVGHCLTGRGEGFLFFGGDVVPSSGMSWTPILRNSE 243
           GVLGL   K +I+SQL   G+V NV+GHCL+  G G LFFG D+  SS +SWTP+ R   
Sbjct: 188 GVLGLGRGKVSILSQLHSQGYVKNVIGHCLSSLGGGILFFGDDLYDSSRVSWTPMSREYS 247

Query: 244 GKYSSG-PAEVYFNGRAVGIGGLTLTFDSGSSYTYFNSQVYRAIEKLLKNDLKGKPLKLA 303
             YS     E+ F GR  G+  L   FDSGSSYTYFNS+ Y+A+  LLK +L GKPLK A
Sbjct: 248 KHYSPAMGGELLFGGRTTGLKNLLTVFDSGSSYTYFNSKAYQAVTYLLKRELSGKPLKEA 307

Query: 304 SDDKTLELCWKGPKPFESVVDVRNFFKPLAMSFKNS--KNVQFQIPPEAYLIISEFGNVC 363
            DD TL LCW+G +PF S+ +V+ +FKPLA+SFK        F+IPPEAYLIIS  GNVC
Sbjct: 308 RDDHTLPLCWQGRRPFMSIEEVKKYFKPLALSFKTGWRSKTLFEIPPEAYLIISMKGNVC 367

Query: 364 LGILDGSKEGMGNVNIIG 379
           LGIL+G++ G+ N+N+IG
Sbjct: 368 LGILNGTEIGLQNLNLIG 383

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q0IU527.8e-8441.60Aspartic proteinase Asp1 OS=Oryza sativa subsp. japonica OX=39947 GN=ASP1 PE=2 S... [more]
A2ZC672.8e-8141.13Aspartic proteinase Asp1 OS=Oryza sativa subsp. indica OX=39946 GN=ASP1 PE=2 SV=... [more]
Q9M9A82.6e-7941.60Aspartyl protease APCB1 OS=Arabidopsis thaliana OX=3702 GN=APCB1 PE=1 SV=1[more]
Q9S9K49.6e-3426.35Aspartic proteinase 39 OS=Arabidopsis thaliana OX=3702 GN=A39 PE=1 SV=2[more]
Q4V3D21.9e-2925.63Aspartic proteinase 36 OS=Arabidopsis thaliana OX=3702 GN=A36 PE=1 SV=1[more]
Match NameE-valueIdentityDescription
A0A0A0L1V46.1e-24199.52Peptidase A1 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_4G36186... [more]
A0A1S3B9B93.0e-22492.25aspartic proteinase Asp1-like OS=Cucumis melo OX=3656 GN=LOC103487600 PE=4 SV=1[more]
A0A5D3E2A06.1e-21790.31Aspartic proteinase Asp1-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_sc... [more]
A0A6J1FCF01.5e-19981.60aspartic proteinase Asp1-like isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111... [more]
A0A6J1IHP12.6e-19981.84aspartic proteinase Asp1-like isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC11147... [more]
Match NameE-valueIdentityDescription
XP_004142630.11.3e-24099.52aspartic proteinase Asp1 [Cucumis sativus] >KGN54552.1 hypothetical protein Csa_... [more]
XP_008444184.16.3e-22492.25PREDICTED: aspartic proteinase Asp1-like [Cucumis melo][more]
TYK30006.11.3e-21690.31aspartic proteinase Asp1-like [Cucumis melo var. makuwa][more]
XP_038896620.18.0e-21185.92aspartic proteinase Asp1-like [Benincasa hispida][more]
KAG6591879.14.0e-20282.57Aspartic proteinase Asp1, partial [Cucurbita argyrosperma subsp. sororia][more]
Match NameE-valueIdentityDescription
AT1G44130.17.1e-12553.35Eukaryotic aspartyl protease family protein [more]
AT1G77480.21.6e-12151.72Eukaryotic aspartyl protease family protein [more]
AT1G77480.11.1e-12051.85Eukaryotic aspartyl protease family protein [more]
AT4G33490.21.5e-11149.50Eukaryotic aspartyl protease family protein [more]
AT4G33490.15.0e-10249.74Eukaryotic aspartyl protease family protein [more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (PI 183967) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001461Aspartic peptidase A1 familyPRINTSPR00792PEPSINcoord: 267..278
score: 27.34
coord: 375..390
score: 32.36
coord: 59..79
score: 43.95
IPR001461Aspartic peptidase A1 familyPANTHERPTHR13683ASPARTYL PROTEASEScoord: 7..406
IPR021109Aspartic peptidase domain superfamilyGENE3D2.40.70.10Acid Proteasescoord: 226..407
e-value: 1.4E-32
score: 114.6
IPR021109Aspartic peptidase domain superfamilyGENE3D2.40.70.10Acid Proteasescoord: 28..225
e-value: 4.4E-37
score: 129.9
IPR021109Aspartic peptidase domain superfamilySUPERFAMILY50630Acid proteasescoord: 45..405
IPR032799Xylanase inhibitor, C-terminalPFAMPF14541TAXi_Ccoord: 267..399
e-value: 5.3E-16
score: 58.8
IPR032861Xylanase inhibitor, N-terminalPFAMPF14543TAXi_Ncoord: 54..225
e-value: 3.3E-38
score: 131.7
NoneNo IPR availablePANTHERPTHR13683:SF227EUKARYOTIC ASPARTYL PROTEASE FAMILY PROTEINcoord: 7..406
IPR033121Peptidase family A1 domainPROSITEPS51767PEPTIDASE_A1coord: 53..399
score: 34.27795

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CSPI04G17710.1CSPI04G17710.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006508 proteolysis
molecular_function GO:0004190 aspartic-type endopeptidase activity