CSPI04G08380 (gene) Cucumber (PI 183967) v1

Overview


Name	CSPI04G08380
Type	gene
Organism	Cucumis sativus var. hardwickii cv. PI 183967 (Cucumber (PI 183967) v1)
Description	Transposon Ty3-G Gag-Pol polyprotein
Location	Chr4: 6137955 .. 6138425 (+)
RNA-Seq Expression	CSPI04G08380
Synteny	CSPI04G08380

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGCACTTGAACAATGAAGAAATGAAATTTAATGTTGCCAACGTGATGAAATTTTCCGTTGATGTTGAGTACTACAGCGTAATAGAAGACCTTGGTTGGGACTATTGTAAGGAAGAAATATATCGAGAACTATTTAGCACTAAAAAATTCTTTAAAGACGAGCTAAAATGGCTTGAAGAAGTAAATGTCGTAATTGGTGAAAAGGAGTGTGAACATCTGGACTTGGAAAACAAAGGTGATAAGGGAACAAAGCCATCGATTGAAGAACCACCAGAACTAGAGCTCAAGTTGTTGCCTCCTCACCCGAAGTATGTATTTTTAGTAGAGAATGACACTCTACCTATCATTACCTTTGCATCATTTAATGGTATTGGAGAAAGGGCATTATTGAACATGCAAAAATGCCAAAAGAAGGCAATAAGATGGAACCTTGCTGATATTCAAGGCATAAGTCCATCAAGATCAGGCTAA

mRNA sequence

ATGCACTTGAACAATGAAGAAATGAAATTTAATGTTGCCAACGTGATGAAATTTTCCGTTGATGTTGAGTACTACAGCGTAATAGAAGACCTTGGTTGGGACTATTGTAAGGAAGAAATATATCGAGAACTATTTAGCACTAAAAAATTCTTTAAAGACGAGCTAAAATGGCTTGAAGAAGTAAATGTCGTAATTGGTGAAAAGGAGTGTGAACATCTGGACTTGGAAAACAAAGGTGATAAGGGAACAAAGCCATCGATTGAAGAACCACCAGAACTAGAGCTCAAGTTGTTGCCTCCTCACCCGAAGTATGTATTTTTAGTAGAGAATGACACTCTACCTATCATTACCTTTGCATCATTTAATGGTATTGGAGAAAGGGCATTATTGAACATGCAAAAATGCCAAAAGAAGGCAATAAGATGGAACCTTGCTGATATTCAAGGCATAAGTCCATCAAGATCAGGCTAA

Coding sequence (CDS)

ATGCACTTGAACAATGAAGAAATGAAATTTAATGTTGCCAACGTGATGAAATTTTCCGTTGATGTTGAGTACTACAGCGTAATAGAAGACCTTGGTTGGGACTATTGTAAGGAAGAAATATATCGAGAACTATTTAGCACTAAAAAATTCTTTAAAGACGAGCTAAAATGGCTTGAAGAAGTAAATGTCGTAATTGGTGAAAAGGAGTGTGAACATCTGGACTTGGAAAACAAAGGTGATAAGGGAACAAAGCCATCGATTGAAGAACCACCAGAACTAGAGCTCAAGTTGTTGCCTCCTCACCCGAAGTATGTATTTTTAGTAGAGAATGACACTCTACCTATCATTACCTTTGCATCATTTAATGGTATTGGAGAAAGGGCATTATTGAACATGCAAAAATGCCAAAAGAAGGCAATAAGATGGAACCTTGCTGATATTCAAGGCATAAGTCCATCAAGATCAGGCTAA

Protein sequence

MHLNNEEMKFNVANVMKFSVDVEYYSVIEDLGWDYCKEEIYRELFSTKKFFKDELKWLEEVNVVIGEKECEHLDLENKGDKGTKPSIEEPPELELKLLPPHPKYVFLVENDTLPIITFASFNGIGERALLNMQKCQKKAIRWNLADIQGISPSRSG*

Homology

[Close]

BLAST of CSPI04G08380 vs. ExPASy TrEMBL
Match: A0A5A7UF79 (Uncharacterized protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold675G00260 PE=4 SV=1)

HSP 1 Score: 145.2 bits (365), Expect = 2.3e-31
Identity = 80/141 (56.74%), Postives = 97/141 (68.79%), Query Frame = 0


Query: 15  VMKFSVDVEYYSVIEDLGWDYCKEEIYRELFSTKKFFK--DELKWLEEVNVVIGEKECEH 74
           +  F  D E  +VIE LGW+Y ++E Y+ELFST++FF+  D    L+EVNVV  EK+ E 
Sbjct: 147 IKDFPTDDENCNVIEFLGWNYFEKEAYQELFSTEEFFEEDDPSYILKEVNVVSSEKKFES 206

Query: 75  LDLENKGDKGTKPSIEEPPELELKLLPPHPKYVFLVENDTLPIITFASFNGIGERALLNM 134
           LDL+ KG+K TKPSIEEPPELELK LP H KYV+L ENDTLP+      +   E+ LLNM
Sbjct: 207 LDLQIKGEKKTKPSIEEPPELELKSLPNHLKYVYLRENDTLPVSISTHLDVAKEKELLNM 266

Query: 135 QKCQKKAIRWNLADIQGISPS 154
            K  KK I W L DIQGIS S
Sbjct: 267 LKRHKKEIEWTLVDIQGISSS 287

[Close]

BLAST of CSPI04G08380 vs. ExPASy TrEMBL
Match: A0A5D3C0Z2 (Integrase_H2C2 domain-containing protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold98G00690 PE=4 SV=1)

HSP 1 Score: 126.7 bits (317), Expect = 8.5e-26
Identity = 68/132 (51.52%), Postives = 89/132 (67.42%), Query Frame = 0


Query: 18  FSVDVEYYSVIEDLGWDYCKEEIYRELFSTKKFFKDELKW--LEEVNVVIGEKECEHLDL 77
           F +DVE  + IE LGWDYC+EE Y ELFSTK+F +++  +  L+EVN V G+ + E LDL
Sbjct: 110 FPIDVESCNAIECLGWDYCEEEAYYELFSTKEFLEEDKPYYILKEVNTVSGKNKFESLDL 169

Query: 78  ENKGDKGTKPSIEEPPELELKLLPPHPKYVFLVENDTLPIITFASFNGIGERALLNMQKC 137
           + KG+  T+ SIEEPP+LELK LP H KY +L ENDTLP+   A  +   E+ALLNM K 
Sbjct: 170 QTKGENKTQASIEEPPKLELKPLPNHLKYAYLRENDTLPVSIPAHLDVTEEKALLNMLKR 229

Query: 138 QKKAIRWNLADI 148
            KK     L ++
Sbjct: 230 HKKECLSTLEEV 241

[Close]

BLAST of CSPI04G08380 vs. ExPASy TrEMBL
Match: A0A5D3C2R8 (Uncharacterized protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold83G002090 PE=4 SV=1)

HSP 1 Score: 125.9 bits (315), Expect = 1.4e-25
Identity = 67/115 (58.26%), Postives = 83/115 (72.17%), Query Frame = 0


Query: 1   MHLNNEEMKFNVANVMKFSVDVEYYSVIEDLGWDYCKEEIYRELFSTKKFFKDE--LKWL 60
           MH N+EE+KFNV N MKF +D + Y+VI+  GW Y KEE+Y ELFSTK+FF++E     L
Sbjct: 29  MHFNDEEVKFNVVNAMKFPIDDKNYTVIKSFGWVYFKEEVYEELFSTKEFFEEEDPNYIL 88

Query: 61  EEVNVVIGEKECEHLDLENKGDKGTKPSIEEPPELELKLLPPHPKYVFLVENDTL 114
           EE N + GE++ E LDL+ KG K  KPSIEE  ELELK LP H KY +L ENDT+
Sbjct: 89  EEENAMSGERKFESLDLQIKGKKKIKPSIEETLELELKPLPTHLKYTYLGENDTV 143

[Close]

BLAST of CSPI04G08380 vs. ExPASy TrEMBL
Match: A0A5A7UE99 (Uncharacterized protein OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold131G00340 PE=4 SV=1)

HSP 1 Score: 125.2 bits (313), Expect = 2.5e-25
Identity = 68/117 (58.12%), Postives = 83/117 (70.94%), Query Frame = 0


Query: 1   MHLNNEEMKFNVANVMKFSVDVEYYSVIEDLGWDYCKEEIYRELFSTKKFFKDE--LKWL 60
           M  N+EE+KFN    MKF  +   Y+ IE LGWDYCKEE Y ELFST++FF++E     L
Sbjct: 76  MCFNDEEIKFNCYQSMKFPANDVKYNAIESLGWDYCKEEPYHELFSTEEFFEEEDPSYIL 135

Query: 61  EEVNVVIGEKECEHLDLENKGDKGTKPSIEEPPELELKLLPPHPKYVFLVENDTLPI 116
           EEVNV++G+ + E LDL+ KG K  K SIEEPPE+ELK LP H KY +L ENDTL I
Sbjct: 136 EEVNVMLGKTKFESLDLKTKGKKKIKSSIEEPPEIELKPLPNHLKYAYLGENDTLYI 192

[Close]

BLAST of CSPI04G08380 vs. ExPASy TrEMBL
Match: A0A5D3BCU9 (Integrase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold220G00570 PE=4 SV=1)

HSP 1 Score: 117.1 bits (292), Expect = 6.7e-23
Identity = 68/118 (57.63%), Postives = 80/118 (67.80%), Query Frame = 0


Query: 38  EEIYRELFSTKKFF-KDELKW-LEEVNVVIGEKECEHLDLENKGDKGTKPSIEEPPELEL 97
           EE Y +LF+ +KF  +DE  + LEEVNVV GEK+ E LDL+ KG+K TK SIEEPPELEL
Sbjct: 69  EESYYQLFTAEKFLEEDEPNYILEEVNVVSGEKKFESLDLQTKGEKKTKSSIEEPPELEL 128

Query: 98  KLLPPHPKYVFLVENDTLPIITFASFNGIGERALLNMQKCQKKAIRWNLADIQGISPS 154
           K    H KY +L  NDTL II     +   E+ LLN+ K  KKA  W LADIQGISPS
Sbjct: 129 KPSLNHLKYAYLGGNDTLSIIISTHLDATEEKVLLNILKLHKKANGWTLADIQGISPS 186

[Close]

BLAST of CSPI04G08380 vs. NCBI nr
Match: KAA0052866.1 (uncharacterized protein E6C27_scaffold33G00230 [Cucumis melo var. makuwa] >TYK04359.1 uncharacterized protein E5676_scaffold675G00260 [Cucumis melo var. makuwa])

HSP 1 Score: 145.2 bits (365), Expect = 4.7e-31
Identity = 80/141 (56.74%), Postives = 97/141 (68.79%), Query Frame = 0


Query: 15  VMKFSVDVEYYSVIEDLGWDYCKEEIYRELFSTKKFFK--DELKWLEEVNVVIGEKECEH 74
           +  F  D E  +VIE LGW+Y ++E Y+ELFST++FF+  D    L+EVNVV  EK+ E 
Sbjct: 147 IKDFPTDDENCNVIEFLGWNYFEKEAYQELFSTEEFFEEDDPSYILKEVNVVSSEKKFES 206

Query: 75  LDLENKGDKGTKPSIEEPPELELKLLPPHPKYVFLVENDTLPIITFASFNGIGERALLNM 134
           LDL+ KG+K TKPSIEEPPELELK LP H KYV+L ENDTLP+      +   E+ LLNM
Sbjct: 207 LDLQIKGEKKTKPSIEEPPELELKSLPNHLKYVYLRENDTLPVSISTHLDVAKEKELLNM 266

Query: 135 QKCQKKAIRWNLADIQGISPS 154
            K  KK I W L DIQGIS S
Sbjct: 267 LKRHKKEIEWTLVDIQGISSS 287

[Close]

BLAST of CSPI04G08380 vs. NCBI nr
Match: KAA0040570.1 (uncharacterized protein E6C27_scaffold262G001400 [Cucumis melo var. makuwa] >TYK05613.1 uncharacterized protein E5676_scaffold98G00690 [Cucumis melo var. makuwa])

HSP 1 Score: 126.7 bits (317), Expect = 1.7e-25
Identity = 68/132 (51.52%), Postives = 89/132 (67.42%), Query Frame = 0


Query: 18  FSVDVEYYSVIEDLGWDYCKEEIYRELFSTKKFFKDELKW--LEEVNVVIGEKECEHLDL 77
           F +DVE  + IE LGWDYC+EE Y ELFSTK+F +++  +  L+EVN V G+ + E LDL
Sbjct: 110 FPIDVESCNAIECLGWDYCEEEAYYELFSTKEFLEEDKPYYILKEVNTVSGKNKFESLDL 169

Query: 78  ENKGDKGTKPSIEEPPELELKLLPPHPKYVFLVENDTLPIITFASFNGIGERALLNMQKC 137
           + KG+  T+ SIEEPP+LELK LP H KY +L ENDTLP+   A  +   E+ALLNM K 
Sbjct: 170 QTKGENKTQASIEEPPKLELKPLPNHLKYAYLRENDTLPVSIPAHLDVTEEKALLNMLKR 229

Query: 138 QKKAIRWNLADI 148
            KK     L ++
Sbjct: 230 HKKECLSTLEEV 241

[Close]

BLAST of CSPI04G08380 vs. NCBI nr
Match: TYK05498.1 (uncharacterized protein E5676_scaffold83G002090 [Cucumis melo var. makuwa])

HSP 1 Score: 125.9 bits (315), Expect = 3.0e-25
Identity = 67/115 (58.26%), Postives = 83/115 (72.17%), Query Frame = 0


Query: 1   MHLNNEEMKFNVANVMKFSVDVEYYSVIEDLGWDYCKEEIYRELFSTKKFFKDE--LKWL 60
           MH N+EE+KFNV N MKF +D + Y+VI+  GW Y KEE+Y ELFSTK+FF++E     L
Sbjct: 29  MHFNDEEVKFNVVNAMKFPIDDKNYTVIKSFGWVYFKEEVYEELFSTKEFFEEEDPNYIL 88

Query: 61  EEVNVVIGEKECEHLDLENKGDKGTKPSIEEPPELELKLLPPHPKYVFLVENDTL 114
           EE N + GE++ E LDL+ KG K  KPSIEE  ELELK LP H KY +L ENDT+
Sbjct: 89  EEENAMSGERKFESLDLQIKGKKKIKPSIEETLELELKPLPTHLKYTYLGENDTV 143

[Close]

BLAST of CSPI04G08380 vs. NCBI nr
Match: KAA0054122.1 (uncharacterized protein E6C27_scaffold131G00340 [Cucumis melo var. makuwa])

HSP 1 Score: 125.2 bits (313), Expect = 5.1e-25
Identity = 68/117 (58.12%), Postives = 83/117 (70.94%), Query Frame = 0


Query: 1   MHLNNEEMKFNVANVMKFSVDVEYYSVIEDLGWDYCKEEIYRELFSTKKFFKDE--LKWL 60
           M  N+EE+KFN    MKF  +   Y+ IE LGWDYCKEE Y ELFST++FF++E     L
Sbjct: 76  MCFNDEEIKFNCYQSMKFPANDVKYNAIESLGWDYCKEEPYHELFSTEEFFEEEDPSYIL 135

Query: 61  EEVNVVIGEKECEHLDLENKGDKGTKPSIEEPPELELKLLPPHPKYVFLVENDTLPI 116
           EEVNV++G+ + E LDL+ KG K  K SIEEPPE+ELK LP H KY +L ENDTL I
Sbjct: 136 EEVNVMLGKTKFESLDLKTKGKKKIKSSIEEPPEIELKPLPNHLKYAYLGENDTLYI 192

[Close]

BLAST of CSPI04G08380 vs. NCBI nr
Match: TYJ96947.1 (integrase [Cucumis melo var. makuwa])

HSP 1 Score: 117.1 bits (292), Expect = 1.4e-22
Identity = 68/118 (57.63%), Postives = 80/118 (67.80%), Query Frame = 0


Query: 38  EEIYRELFSTKKFF-KDELKW-LEEVNVVIGEKECEHLDLENKGDKGTKPSIEEPPELEL 97
           EE Y +LF+ +KF  +DE  + LEEVNVV GEK+ E LDL+ KG+K TK SIEEPPELEL
Sbjct: 69  EESYYQLFTAEKFLEEDEPNYILEEVNVVSGEKKFESLDLQTKGEKKTKSSIEEPPELEL 128

Query: 98  KLLPPHPKYVFLVENDTLPIITFASFNGIGERALLNMQKCQKKAIRWNLADIQGISPS 154
           K    H KY +L  NDTL II     +   E+ LLN+ K  KKA  W LADIQGISPS
Sbjct: 129 KPSLNHLKYAYLGGNDTLSIIISTHLDATEEKVLLNILKLHKKANGWTLADIQGISPS 186

The following BLAST results are available for this feature:

BLAST of CSPI04G08380 vs.


Match Name	E-value	Identity	Description

BLAST of CSPI04G08380 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A5A7UF79	2.3e-31	56.74	Uncharacterized protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold...	[more]
A0A5D3C0Z2	8.5e-26	51.52	Integrase_H2C2 domain-containing protein OS=Cucumis melo var. makuwa OX=1194695 ...	[more]
A0A5D3C2R8	1.4e-25	58.26	Uncharacterized protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold...	[more]
A0A5A7UE99	2.5e-25	58.12	Uncharacterized protein OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold...	[more]
A0A5D3BCU9	6.7e-23	57.63	Integrase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold220G00570 PE=4...	[more]

BLAST of CSPI04G08380 vs. NCBI nr


Match Name	E-value	Identity	Description
KAA0052866.1	4.7e-31	56.74	uncharacterized protein E6C27_scaffold33G00230 [Cucumis melo var. makuwa] >TYK04...	[more]
KAA0040570.1	1.7e-25	51.52	uncharacterized protein E6C27_scaffold262G001400 [Cucumis melo var. makuwa] >TYK...	[more]
TYK05498.1	3.0e-25	58.26	uncharacterized protein E5676_scaffold83G002090 [Cucumis melo var. makuwa]	[more]
KAA0054122.1	5.1e-25	58.12	uncharacterized protein E6C27_scaffold131G00340 [Cucumis melo var. makuwa]	[more]
TYJ96947.1	1.4e-22	57.63	integrase [Cucumis melo var. makuwa]	[more]

BLAST of CSPI04G08380 vs. NCBI nr


Match Name	E-value	Identity	Description

InterPro

Analysis Name: InterPro Annotations of Cucumber (PI 183967) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	PANTHER	PTHR24559	TRANSPOSON TY3-I GAG-POL POLYPROTEIN	coord: 67..153
None	No IPR available	PANTHER	PTHR24559:SF373	REVERSE TRANSCRIPTASE DOMAIN, RIBONUCLEASE H-LIKE DOMAIN PROTEIN-RELATED	coord: 67..153

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CSPI04G08380.1	CSPI04G08380.1	mRNA