CSPI03G26650 (gene) Cucumber (PI 183967) v1

Overview
NameCSPI03G26650
Typegene
OrganismCucumis sativus var. hardwickii cv. PI 183967 (Cucumber (PI 183967) v1)
Descriptionthioredoxin-like fold domain-containing protein MRL7, chloroplastic
LocationChr3: 24084920 .. 24088572 (-)
RNA-Seq ExpressionCSPI03G26650
SyntenyCSPI03G26650
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
CAAAATTTACCAATATTTTATCCTTTGGATATTGGATTGGAATTGTTCTCATAAACCCTCAATTGAAGCTCTCATCATTTCATAAACCCCACTGTCTTCATCGTCATCCTTCATCCTTCATTCTTCATTCTTCTCTCTATCTTGATAATTTACACCAGGGAGCTCCAAACTTCTTCACACAGAGCATTTTCCCCGTTTTCTATTGGCACCCAGTTATGGTATGTTCAAAATTACTTACAAACTTGCTCAATTTTGGGTTCTTTTACTTTACTTATGCCTCCTTCATCTCTTCGATTTGCTTATCTTTCATTTTTCGTACACAAGTGTTGTTAAGTAGCATCTTGTAGGTCTGAAGAAAGGAAAATTTGGATTTGGGATTAAATTATGAGGTGTTTCCTCATGTTTAGTTTTAAGACGTCTCTTCGTTCTGTGAATTTGACGCCTAGAGATGAAGTTAGCCAGGGAATGTGAGTATAAAAGAGAGATTATTGAGAGAAAATATATGTCTACAAATATGCTTGGGAGAGGAAGTTTGTACGAAGAAATTGGTATAATTTGTGTAGGAATGTGTAATGATAAGGTAGTGAGAGCCTATTGTCCCAACTTAATTGGTTTACATAACCAAGAGATCAGGTGTTTGAATCCTCCGACTCCTGTATGGTTGAACTCAAAATAAGAGAAGTAGTGAGAAAATGTATATGATGAGAAAGATTATTGAGAAAGGATTTGTGGAGACAAACTACATAGAAAATGGAGTGTCTAAGCCCGTCTGTTTAAGTACTTTACGAGTTTCTTATTTGACACGCACTACTTTGGTTATTTTGGAAAGCTCTTCTGGACTTGTAAAGGTCCGTTTGATAACCATTTGGTTTTTGGTTTTTATTTTTTGAAAAGTTAAGCCCATGAACACCCTTCACCCATGAGTTTGTTGCCTTATTTTCTACTTTCTACCGATGTTTTCAAAAACTAAATCAAAATTTGAATACTAAAAAGGTAGATTTTAAAGACTTGCATTTGTTTTTGAAATTTGGCTTAGAGTTCTCTTTTCCTTCACAAATCTTTTTAGAGGAAGTAGACTACATTTTCAAAAACCAAAAAGCCAAAGCCAGATGGTTACTTAAAGGGGCATAAGTATTTGAATTTGAAAGTGTTTTCAATCACCTAAAAATATTTCTACTATGTTAGAAAAGCACTCCCTGCATCACTTTAGCTTCGGGTTTTTGTCAGTTTGATAATTGATTGTTGTAAAGTCTGAAGACTCTTAAGAGAGAACAAATGAAGCATGTTCTAAGGATTGTTAAAAATTAATTTACGATGTTGGTTTATGGAATTAATTTGATTTCTATGGTTAGGTATTGACATAAAGTGGACGCGTAAGCTTAAGAGGAGACTTGAGTTGAAGTTTTCAGTTAAGTCATGGTCTAACTGCAGAGTAATGGCCTAATAATGGGATATTTGTGAGTGGCTTTGTTTTTGTTTAGTTTTGATATGGATTTTGGTCTCATATGCGTTCCAAACATCCATGCCCCTGTTGCTCCATGTGCTTAACAGGCTTCAACCTATGCTAGTCTGGAAATAGAGAAAATGTTGATTCTCCAAGCCTCTTTCATCTCAATGACGGTTTCACCTCAATATGTAGTTCATAATGCTTTTACTTTGCGTCGATTTTTAACAGCTCCTACTGAATATCAAACTTTTAAGCCTTCTTGTTATTTTAGATATCATTCAGCCAGTATTTTGCCGAATGTTGTACACCATAGAAACAGCCGAAAACTTCCTTGCCTTGCTGTTTCGTCTAAATCTAATTCAGAACATGATCCTGAACCTAGTGCTGATTCTAAAGTAAAACCAAAACCGATAAGTCGCATTAAGAGAAGCAAAACTTCTTCTGAAAAGGAGGAAGAAAATGGTGCTGGGATTTTTCCAACAACAATCCCAAGGAAGCCTCGACGTGGTCGTAGAAGTGAAGCTGCAGCTCTTGAAGATTATGTTCGCAACACGCTTGAACAAACATTTGCATCTATCCGACAGCAGAATGCAGAAGTAATGGAAAATAAGGAAAATGTATTCAAGGAAAAGGTTGATAATGAGGTTGAATCTGAAAGTAGTGATGATGAAGATGATGCGGACGAAGAAGAGGATGTAGAACAAAATGATGGAGCTGGAAGGAATAAGAAGAAGAAGATGGTGGTAGAGGAAGAAGATCCAAATTGGCCGCTGGATGCTGATGTGGGATGGGGAGTTCGAGCCTCGGAGTATTTTGAACAGCACCCTATCAAGAATGTAGTTGGGGATGATGGTGTCGAAATTGACTGGGAAGGGGAAATTGATGACAGTTGGGTGCAGGAAATCAACTGTTTGGAGTGGGAGAGCTTTGCTTTTCATCCTAGTCCACTTGTTGTTCTTGTTTTTGAAAGATATAACAGGTTGCTTTCTTCTACCTTTTGAAATTTTCGAAATTTGTGATTCTTACGTCAATAATGTGTTAAAAGTATATTGAAAATAATGATACTAATACAATGGGCTTCAACGTTTTATAGATTATTTAATGAACATGATGCAACATGCATTCTTGTAATACTACAAATTGGGATAAATGATAATACTTTAAGATCTATGTGCCATATTTTGAAATTTCTTGCAGATCATATTCCATGTTGAATCATTGATTTTTCTGTGCAGGGCGACTGATAATTGGAAGACTTTGAAAGAACTTGAGACAGCACTGAAAGTGTACTGGAATGCAAAGGATCGATTACCACCCCGGGTATGAACTAAGTTGCATGTTTTTTAGCTGTTTGATTTCACAAAATTTAAATCCTGATAAGTCGAATCCTCGAGGCAAATCATTTGATTCATACTCAGTCTACCTGCCCTCTCCCCATAACAAACTTTTTGTTTCATATGTTTAAATATTGTTCTGTTATGTCAGTTGAATTTCTCCCATTTTTCTTCTGTTGATATTCTCATAATGATTTTTATACCTGATCTGTGTATTCAATGATTTCACTATTTATTTTACCTAGTATCTGTTGATTATTGTGTTTTTAAACTTGTGCTTCAGTCAGTTAAGATTGATATCAATATCGAGCGCGATCTAGCCTATGCTTTGAAAGTTAGAGAATGCCCTCAAATTCTATTTTTAAGAGGAAACAAGGTCTTATACAGAGAAAAAGGTCTACATTTTGTTTCCTGCTACATTTCATTCCAGTTTGTGCAAATATTTCTTTTAAACTTAAACCGACACGTGTGCCTTTTTTGATATATTCTTTTGCAGACTTTCGGACGGCAGATGAATTGGTTCAGATGATTGCATTTTTCTATTACAAAGCAAAGAAGCCTTCATGGATCAATGATAAAGCTCTAGCTAGACCATTTTGAGCTCGAATCTCTTCCTCCTACTTGGACATACGCTCCAAGGTCAGAGGATTTATAGACATCAGGTAGGTTTGATTTCAACCAACAAAATAGTTTATGAATACCATACTGGATTTGGAGTTATTTCAAATACAAACATGCTTTGAGTTTAAAATCAACATGTGATATAGCTCAAGTAAGTGGAGTTTAAGTGTCTTCCCACTTGACTACATAATTCTTGTATGTTTAAGTTTTTTCGTTCGGTGGTGTAATATTGGGGAAAATTAATCTCAAGC

mRNA sequence

CAAAATTTACCAATATTTTATCCTTTGGATATTGGATTGGAATTGTTCTCATAAACCCTCAATTGAAGCTCTCATCATTTCATAAACCCCACTGTCTTCATCGTCATCCTTCATCCTTCATTCTTCATTCTTCTCTCTATCTTGATAATTTACACCAGGGAGCTCCAAACTTCTTCACACAGAGCATTTTCCCCGTTTTCTATTGGCACCCAGTTATGGTATTGACATAAAGTGGACGCGTAAGCTTAAGAGGAGACTTGAGTTGAAGTTTTCAGTTAAGTCATGGTCTAACTGCAGAGTAATGGCCTAATAATGGGATATTTGTGAGTGGCTTTGTTTTTGTTTAGTTTTGATATGGATTTTGGTCTCATATGCGTTCCAAACATCCATGCCCCTGTTGCTCCATGTGCTTAACAGGCTTCAACCTATGCTAGTCTGGAAATAGAGAAAATGTTGATTCTCCAAGCCTCTTTCATCTCAATGACGGTTTCACCTCAATATGTAGTTCATAATGCTTTTACTTTGCGTCGATTTTTAACAGCTCCTACTGAATATCAAACTTTTAAGCCTTCTTGTTATTTTAGATATCATTCAGCCAGTATTTTGCCGAATGTTGTACACCATAGAAACAGCCGAAAACTTCCTTGCCTTGCTGTTTCGTCTAAATCTAATTCAGAACATGATCCTGAACCTAGTGCTGATTCTAAAGTAAAACCAAAACCGATAAGTCGCATTAAGAGAAGCAAAACTTCTTCTGAAAAGGAGGAAGAAAATGGTGCTGGGATTTTTCCAACAACAATCCCAAGGAAGCCTCGACGTGGTCGTAGAAGTGAAGCTGCAGCTCTTGAAGATTATGTTCGCAACACGCTTGAACAAACATTTGCATCTATCCGACAGCAGAATGCAGAAGTAATGGAAAATAAGGAAAATGTATTCAAGGAAAAGGTTGATAATGAGGTTGAATCTGAAAGTAGTGATGATGAAGATGATGCGGACGAAGAAGAGGATGTAGAACAAAATGATGGAGCTGGAAGGAATAAGAAGAAGAAGATGGTGGTAGAGGAAGAAGATCCAAATTGGCCGCTGGATGCTGATGTGGGATGGGGAGTTCGAGCCTCGGAGTATTTTGAACAGCACCCTATCAAGAATGTAGTTGGGGATGATGGTGTCGAAATTGACTGGGAAGGGGAAATTGATGACAGTTGGGTGCAGGAAATCAACTGTTTGGAGTGGGAGAGCTTTGCTTTTCATCCTAGTCCACTTGTTGTTCTTGTTTTTGAAAGATATAACAGGGCGACTGATAATTGGAAGACTTTGAAAGAACTTGAGACAGCACTGAAAGTGTACTGGAATGCAAAGGATCGATTACCACCCCGGTCAGTTAAGATTGATATCAATATCGAGCGCGATCTAGCCTATGCTTTGAAAGTTAGAGAATGCCCTCAAATTCTATTTTTAAGAGGAAACAAGGTCTTATACAGAGAAAAAGACTTTCGGACGGCAGATGAATTGGTTCAGATGATTGCATTTTTCTATTACAAAGCAAAGAAGCCTTCATGGATCAATGATAAAGCTCTAGCTAGACCATTTTGAGCTCGAATCTCTTCCTCCTACTTGGACATACGCTCCAAGGTCAGAGGATTTATAGACATCAGGTAGGTTTGATTTCAACCAACAAAATAGTTTATGAATACCATACTGGATTTGGAGTTATTTCAAATACAAACATGCTTTGAGTTTAAAATCAACATGTGATATAGCTCAAGTAAGTGGAGTTTAAGTGTCTTCCCACTTGACTACATAATTCTTGTATGTTTAAGTTTTTTCGTTCGGTGGTGTAATATTGGGGAAAATTAATCTCAAGC

Coding sequence (CDS)

ATGTTGATTCTCCAAGCCTCTTTCATCTCAATGACGGTTTCACCTCAATATGTAGTTCATAATGCTTTTACTTTGCGTCGATTTTTAACAGCTCCTACTGAATATCAAACTTTTAAGCCTTCTTGTTATTTTAGATATCATTCAGCCAGTATTTTGCCGAATGTTGTACACCATAGAAACAGCCGAAAACTTCCTTGCCTTGCTGTTTCGTCTAAATCTAATTCAGAACATGATCCTGAACCTAGTGCTGATTCTAAAGTAAAACCAAAACCGATAAGTCGCATTAAGAGAAGCAAAACTTCTTCTGAAAAGGAGGAAGAAAATGGTGCTGGGATTTTTCCAACAACAATCCCAAGGAAGCCTCGACGTGGTCGTAGAAGTGAAGCTGCAGCTCTTGAAGATTATGTTCGCAACACGCTTGAACAAACATTTGCATCTATCCGACAGCAGAATGCAGAAGTAATGGAAAATAAGGAAAATGTATTCAAGGAAAAGGTTGATAATGAGGTTGAATCTGAAAGTAGTGATGATGAAGATGATGCGGACGAAGAAGAGGATGTAGAACAAAATGATGGAGCTGGAAGGAATAAGAAGAAGAAGATGGTGGTAGAGGAAGAAGATCCAAATTGGCCGCTGGATGCTGATGTGGGATGGGGAGTTCGAGCCTCGGAGTATTTTGAACAGCACCCTATCAAGAATGTAGTTGGGGATGATGGTGTCGAAATTGACTGGGAAGGGGAAATTGATGACAGTTGGGTGCAGGAAATCAACTGTTTGGAGTGGGAGAGCTTTGCTTTTCATCCTAGTCCACTTGTTGTTCTTGTTTTTGAAAGATATAACAGGGCGACTGATAATTGGAAGACTTTGAAAGAACTTGAGACAGCACTGAAAGTGTACTGGAATGCAAAGGATCGATTACCACCCCGGTCAGTTAAGATTGATATCAATATCGAGCGCGATCTAGCCTATGCTTTGAAAGTTAGAGAATGCCCTCAAATTCTATTTTTAAGAGGAAACAAGGTCTTATACAGAGAAAAAGACTTTCGGACGGCAGATGAATTGGTTCAGATGATTGCATTTTTCTATTACAAAGCAAAGAAGCCTTCATGGATCAATGATAAAGCTCTAGCTAGACCATTTTGA

Protein sequence

MLILQASFISMTVSPQYVVHNAFTLRRFLTAPTEYQTFKPSCYFRYHSASILPNVVHHRNSRKLPCLAVSSKSNSEHDPEPSADSKVKPKPISRIKRSKTSSEKEEENGAGIFPTTIPRKPRRGRRSEAAALEDYVRNTLEQTFASIRQQNAEVMENKENVFKEKVDNEVESESSDDEDDADEEEDVEQNDGAGRNKKKKMVVEEEDPNWPLDADVGWGVRASEYFEQHPIKNVVGDDGVEIDWEGEIDDSWVQEINCLEWESFAFHPSPLVVLVFERYNRATDNWKTLKELETALKVYWNAKDRLPPRSVKIDINIERDLAYALKVRECPQILFLRGNKVLYREKDFRTADELVQMIAFFYYKAKKPSWINDKALARPF*
Homology
BLAST of CSPI03G26650 vs. ExPASy Swiss-Prot
Match: F4JLC1 (Thioredoxin-like fold domain-containing protein MRL7, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=MRL7 PE=1 SV=1)

HSP 1 Score: 377.5 bits (968), Expect = 1.8e-103
Identity = 199/330 (60.30%), Postives = 245/330 (74.24%), Query Frame = 0

Query: 51  ILPNVVHHRNSRKLPCLAVSSKSNSEHDPEPSADSKVKPKPISRIKRSKTSSEKEEENGA 110
           + P  +  R+ ++   ++    S+S  DPE    SK + +   + K + T   + E N  
Sbjct: 34  VFPVTISSRSVKRFAAVS----SDSVLDPE----SKNQTRSRRKNKEAVTPIAETENNEK 93

Query: 111 GIFPTTIPRKPRRGRRSEAAALEDYVRNTLEQTFASIRQQNAEVMENKENVFKEKVDNEV 170
             FPT +PRK +RGRRSEA A+EDYVR++LE+TF++I++QN EV ENKE        N +
Sbjct: 94  --FPTKVPRKSKRGRRSEADAVEDYVRSSLERTFSTIKEQNPEVFENKEKA------NFI 153

Query: 171 ESESSDDEDDADEEEDVEQNDGAGRNKKKKMVVEEEDPNWPLDADVGWGVRASEYFEQHP 230
           +    D+E++ +EE                MVVEEEDP+WP+D DVGWG++ASEYF+ HP
Sbjct: 154 KDRGVDEEEEEEEE----------------MVVEEEDPDWPVDTDVGWGIKASEYFDTHP 213

Query: 231 IKNVVGDDGVEIDWEGEIDDSWVQEINCLEWESFAFHPSPLVVLVFERYNRATDNWKTLK 290
           IKNVVGDDG EIDWEGEIDDSWV+EINCLEWESFAFHPSPLVVLVFERY RA+DNWKTLK
Sbjct: 214 IKNVVGDDGSEIDWEGEIDDSWVKEINCLEWESFAFHPSPLVVLVFERYKRASDNWKTLK 273

Query: 291 ELETALKVYWNAKDRLPPRSVKIDINIERDLAYALKVRECPQILFLRGNKVLYREKDFRT 350
           ELE A+KVYW+AKDRLPPR+VKID+NIE DLAYALK +ECPQILFLRGN++LYREKDFRT
Sbjct: 274 ELEKAIKVYWDAKDRLPPRAVKIDLNIETDLAYALKAKECPQILFLRGNRILYREKDFRT 330

Query: 351 ADELVQMIAFFYYKAKKPSWINDKALARPF 381
           ADELV MIA FYYKAK+PS + DKA   P+
Sbjct: 334 ADELVHMIAHFYYKAKRPSCV-DKANVTPY 330

BLAST of CSPI03G26650 vs. ExPASy Swiss-Prot
Match: Q5QLS7 (Thioredoxin-like fold domain-containing protein MRL7 homolog, chloroplastic OS=Oryza sativa subsp. japonica OX=39947 GN=MRL7 PE=2 SV=1)

HSP 1 Score: 365.5 bits (937), Expect = 7.1e-100
Identity = 190/327 (58.10%), Postives = 233/327 (71.25%), Query Frame = 0

Query: 54  NVVHHRNSRKLPCLAVSSKSNSEHDPEPSADSKVKPKPISRIKRSKTSSEKEEENGAGIF 113
           N +H R     P   ++S +     P P       PKP  R        +  +      F
Sbjct: 20  NPIHGRRHGHHPSRLLASSAPPPPPPRP-------PKPEPRTSHENLGDDTPD------F 79

Query: 114 PTTIPRKPRRGRRSEAAALEDYVRNTLEQTFASIRQQNAEVMENKENVFKEKVDNEVESE 173
           PTT PRKPRRGRRSEAAA+ED+VR  LEQ FASIR+++ EV+E K ++ K K        
Sbjct: 80  PTTKPRKPRRGRRSEAAAVEDFVRGRLEQVFASIRERDPEVLEGKGDILKRK-------- 139

Query: 174 SSDDEDDADEEEDVEQNDGAGRNK-KKKMVVEEEDPNWPLDADVGWGVRASEYFEQHPIK 233
                   +E  D E  +G G  + + K VVEEEDP+WPLDAD+GWG+RASEYF++H IK
Sbjct: 140 --------EELSDEEGKEGTGEEEGELKAVVEEEDPSWPLDADIGWGIRASEYFDKHSIK 199

Query: 234 NVVGDDGVEIDWEGEIDDSWVQEINCLEWESFAFHPSPLVVLVFERYNRATDNWKTLKEL 293
           NV   DGVEIDWE E+++ WV+EINCLEWESFAFHPSPL+VLVFERYNRA DNWK L+EL
Sbjct: 200 NVT-VDGVEIDWEREVEEGWVKEINCLEWESFAFHPSPLIVLVFERYNRAADNWKFLQEL 259

Query: 294 ETALKVYWNAKDRLPPRSVKIDINIERDLAYALKVRECPQILFLRGNKVLYREKDFRTAD 353
           E A KVYWN+KDRLPPR+VK+D+NIERDLA+AL+V+ECPQ+LFLRGNK+LYREK+ RTAD
Sbjct: 260 EKAAKVYWNSKDRLPPRTVKVDMNIERDLAFALQVKECPQLLFLRGNKILYREKELRTAD 316

Query: 354 ELVQMIAFFYYKAKKPSWINDKALARP 380
           ELVQMIA FYY AK+PS +N +A+A P
Sbjct: 320 ELVQMIAHFYYNAKRPSCVNPEAIAPP 316

BLAST of CSPI03G26650 vs. ExPASy Swiss-Prot
Match: Q9SKB6 (Thioredoxin-like fold domain-containing protein MRL7L, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=MRL7L PE=1 SV=1)

HSP 1 Score: 136.3 bits (342), Expect = 7.0e-31
Identity = 108/271 (39.85%), Postives = 145/271 (53.51%), Query Frame = 0

Query: 125 RRSEAAALEDYVRNTLEQTFASIRQQNAEVMENKENVFKEKVDNEVESESSDDEDDADEE 184
           ++ E +  ED      E+TF        + +E +E     K   EV  +  D E+  DEE
Sbjct: 78  KKEEDSDSEDEEDEVKEETFGGKEASLDDPVERRE---WRKTIREVIDKHPDIEE--DEE 137

Query: 185 EDVEQNDGAGRNKKKK------MVVEEEDPNWPLDADVGWGVRASEYFEQHPIKN----- 244
            D+ +     R K +K      +VV EEDPNWP DAD GWG   +++F +  IKN     
Sbjct: 138 IDMVEK----RRKMQKLLADYPLVVNEEDPNWPEDAD-GWGFSFNQFFNKITIKNEKKEE 197

Query: 245 ------VVGDDG-VEIDWEGEIDDSWVQEINCL---EWESFAFHP-SPLVVLVFERYNRA 304
                   GDD   EI W+   DD++++ I  L   EWE   F   SPL+VLV  RY R 
Sbjct: 198 EDDDEDSEGDDSEKEIVWQ---DDNYIRPIKDLTTAEWEEAVFKDISPLMVLVHNRYKRP 257

Query: 305 TDNWKTLKELETALKVYWNAKDRLP-PRSVKIDINIERDLAYALKVRECPQILFLRGNKV 364
            +N K  +ELE A++V WN    LP PR V +D  +E DL  ALKV   P+I+F +  K+
Sbjct: 258 KENEKFREELEKAIQVIWNC--GLPSPRCVAVDAVVETDLVSALKVSVFPEIIFTKAGKI 317

Query: 365 LYREKDFRTADELVQMIAFFYYKAKKPSWIN 373
           LYREK  RTADEL +++AFFYY A KP  +N
Sbjct: 318 LYREKGIRTADELSKIMAFFYYGAAKPPCLN 333

BLAST of CSPI03G26650 vs. ExPASy Swiss-Prot
Match: Q0DI48 (Thioredoxin-like fold domain-containing protein MRL7L homolog, chloroplastic OS=Oryza sativa subsp. japonica OX=39947 GN=MRL7L PE=2 SV=1)

HSP 1 Score: 116.7 bits (291), Expect = 5.7e-25
Identity = 87/250 (34.80%), Postives = 126/250 (50.40%), Query Frame = 0

Query: 167 DNEVESESSDDEDD------ADEE----------------EDVEQNDGAGRNKKK----- 226
           D+  ESESSDDEDD       DE+                E  E  D A R  K      
Sbjct: 69  DDGSESESSDDEDDDEPMQMTDEQRRTLRRKIREMMDRVPETAEITDPAERKAKMLELLT 128

Query: 227 --KMVVEEEDPNWPLDADVGWGVRASEYFEQHPIK-NVVGDDGVEIDWEGEIDDS---W- 286
             ++VVEEEDPNWP D + G G    ++F++  IK     DD  E D +G   D    W 
Sbjct: 129 KYQLVVEEEDPNWPEDDEDGHGFSLGQFFDKITIKAEKKNDDDEEDDAKGNQSDKEIVWE 188

Query: 287 -------VQEINCLEWESFAFHP-SPLVVLVFERYNRATDNWKTLKELETALKVYWNAKD 346
                  ++++  ++W+   F    PL+VLV  RY R  DN     +L  A++++W  + 
Sbjct: 189 DDNYIKPIRDVKTMDWDDTVFTDFGPLIVLVHNRYKRPQDNENARDQLVKAIEMFW--EY 248

Query: 347 RLP-PRSVKIDINIERDLAYALKVRECPQILFLRGNKVLYREKDFRTADELVQMIAFFYY 374
            LP PR V +D   E DL  AL V   P++LF    K+++R+K  R+A+E  +M+AFFYY
Sbjct: 249 NLPSPRCVAVDACAEPDLVKALNVSGFPEVLFTNAGKIVHRDKVVRSAEEWTRMMAFFYY 308

BLAST of CSPI03G26650 vs. ExPASy TrEMBL
Match: A0A0A0L8M2 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G576330 PE=4 SV=1)

HSP 1 Score: 753.4 bits (1944), Expect = 4.5e-214
Identity = 380/380 (100.00%), Postives = 380/380 (100.00%), Query Frame = 0

Query: 1   MLILQASFISMTVSPQYVVHNAFTLRRFLTAPTEYQTFKPSCYFRYHSASILPNVVHHRN 60
           MLILQASFISMTVSPQYVVHNAFTLRRFLTAPTEYQTFKPSCYFRYHSASILPNVVHHRN
Sbjct: 1   MLILQASFISMTVSPQYVVHNAFTLRRFLTAPTEYQTFKPSCYFRYHSASILPNVVHHRN 60

Query: 61  SRKLPCLAVSSKSNSEHDPEPSADSKVKPKPISRIKRSKTSSEKEEENGAGIFPTTIPRK 120
           SRKLPCLAVSSKSNSEHDPEPSADSKVKPKPISRIKRSKTSSEKEEENGAGIFPTTIPRK
Sbjct: 61  SRKLPCLAVSSKSNSEHDPEPSADSKVKPKPISRIKRSKTSSEKEEENGAGIFPTTIPRK 120

Query: 121 PRRGRRSEAAALEDYVRNTLEQTFASIRQQNAEVMENKENVFKEKVDNEVESESSDDEDD 180
           PRRGRRSEAAALEDYVRNTLEQTFASIRQQNAEVMENKENVFKEKVDNEVESESSDDEDD
Sbjct: 121 PRRGRRSEAAALEDYVRNTLEQTFASIRQQNAEVMENKENVFKEKVDNEVESESSDDEDD 180

Query: 181 ADEEEDVEQNDGAGRNKKKKMVVEEEDPNWPLDADVGWGVRASEYFEQHPIKNVVGDDGV 240
           ADEEEDVEQNDGAGRNKKKKMVVEEEDPNWPLDADVGWGVRASEYFEQHPIKNVVGDDGV
Sbjct: 181 ADEEEDVEQNDGAGRNKKKKMVVEEEDPNWPLDADVGWGVRASEYFEQHPIKNVVGDDGV 240

Query: 241 EIDWEGEIDDSWVQEINCLEWESFAFHPSPLVVLVFERYNRATDNWKTLKELETALKVYW 300
           EIDWEGEIDDSWVQEINCLEWESFAFHPSPLVVLVFERYNRATDNWKTLKELETALKVYW
Sbjct: 241 EIDWEGEIDDSWVQEINCLEWESFAFHPSPLVVLVFERYNRATDNWKTLKELETALKVYW 300

Query: 301 NAKDRLPPRSVKIDINIERDLAYALKVRECPQILFLRGNKVLYREKDFRTADELVQMIAF 360
           NAKDRLPPRSVKIDINIERDLAYALKVRECPQILFLRGNKVLYREKDFRTADELVQMIAF
Sbjct: 301 NAKDRLPPRSVKIDINIERDLAYALKVRECPQILFLRGNKVLYREKDFRTADELVQMIAF 360

Query: 361 FYYKAKKPSWINDKALARPF 381
           FYYKAKKPSWINDKALARPF
Sbjct: 361 FYYKAKKPSWINDKALARPF 380

BLAST of CSPI03G26650 vs. ExPASy TrEMBL
Match: A0A5A7T3E0 (Polyadenylate-binding protein 2-binding protein 2 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold36G001700 PE=4 SV=1)

HSP 1 Score: 716.1 bits (1847), Expect = 7.9e-203
Identity = 361/380 (95.00%), Postives = 369/380 (97.11%), Query Frame = 0

Query: 1   MLILQASFISMTVSPQYVVHNAFTLRRFLTAPTEYQTFKPSCYFRYHSASILPNVVHHRN 60
           MLILQASFISMTVSPQYV+HNA TLRRFLT PTEYQTFKPSCYFRYHSASILPN+VHHRN
Sbjct: 67  MLILQASFISMTVSPQYVLHNASTLRRFLTVPTEYQTFKPSCYFRYHSASILPNLVHHRN 126

Query: 61  SRKLPCLAVSSKSNSEHDPEPSADSKVKPKPISRIKRSKTSSEKEEENGAGIFPTTIPRK 120
           SRKLPC AVSSKSNSEHDPEPSADSKVKPK  SRIKRSK SS KEEENGAGIFPTTIPRK
Sbjct: 127 SRKLPCFAVSSKSNSEHDPEPSADSKVKPKSRSRIKRSKASSGKEEENGAGIFPTTIPRK 186

Query: 121 PRRGRRSEAAALEDYVRNTLEQTFASIRQQNAEVMENKENVFKEKVDNEVESESSDDEDD 180
           PRRGRRSEAAALEDYVRNTLEQTFASIRQQNAEVMENK NVFKEKVDNEVESESSDDE+D
Sbjct: 187 PRRGRRSEAAALEDYVRNTLEQTFASIRQQNAEVMENKGNVFKEKVDNEVESESSDDEED 246

Query: 181 ADEEEDVEQNDGAGRNKKKKMVVEEEDPNWPLDADVGWGVRASEYFEQHPIKNVVGDDGV 240
            DEEEDVEQNDGAGRNKKKKMVVEEEDPNWPLDADVGWGVRASEYFEQ+PIKNVVGDDGV
Sbjct: 247 EDEEEDVEQNDGAGRNKKKKMVVEEEDPNWPLDADVGWGVRASEYFEQYPIKNVVGDDGV 306

Query: 241 EIDWEGEIDDSWVQEINCLEWESFAFHPSPLVVLVFERYNRATDNWKTLKELETALKVYW 300
           +IDWEGEIDD+WVQEINCLEWESFAFHPSPLVVLVFERYNRATDNWKTLKELE AL+VYW
Sbjct: 307 KIDWEGEIDDNWVQEINCLEWESFAFHPSPLVVLVFERYNRATDNWKTLKELEKALQVYW 366

Query: 301 NAKDRLPPRSVKIDINIERDLAYALKVRECPQILFLRGNKVLYREKDFRTADELVQMIAF 360
           NAKDRLPPRSVKIDINIERDLAYALKVRECPQILFLRGNKVLYREKDFRTADELVQMIAF
Sbjct: 367 NAKDRLPPRSVKIDINIERDLAYALKVRECPQILFLRGNKVLYREKDFRTADELVQMIAF 426

Query: 361 FYYKAKKPSWINDKALARPF 381
           FYYKAKKP WINDKAL+RPF
Sbjct: 427 FYYKAKKPLWINDKALSRPF 446

BLAST of CSPI03G26650 vs. ExPASy TrEMBL
Match: A0A1S4DWT5 (uncharacterized protein LOC103489851 OS=Cucumis melo OX=3656 GN=LOC103489851 PE=4 SV=1)

HSP 1 Score: 716.1 bits (1847), Expect = 7.9e-203
Identity = 361/380 (95.00%), Postives = 369/380 (97.11%), Query Frame = 0

Query: 1   MLILQASFISMTVSPQYVVHNAFTLRRFLTAPTEYQTFKPSCYFRYHSASILPNVVHHRN 60
           MLILQASFISMTVSPQYV+HNA TLRRFLT PTEYQTFKPSCYFRYHSASILPN+VHHRN
Sbjct: 13  MLILQASFISMTVSPQYVLHNASTLRRFLTVPTEYQTFKPSCYFRYHSASILPNLVHHRN 72

Query: 61  SRKLPCLAVSSKSNSEHDPEPSADSKVKPKPISRIKRSKTSSEKEEENGAGIFPTTIPRK 120
           SRKLPC AVSSKSNSEHDPEPSADSKVKPK  SRIKRSK SS KEEENGAGIFPTTIPRK
Sbjct: 73  SRKLPCFAVSSKSNSEHDPEPSADSKVKPKSRSRIKRSKASSGKEEENGAGIFPTTIPRK 132

Query: 121 PRRGRRSEAAALEDYVRNTLEQTFASIRQQNAEVMENKENVFKEKVDNEVESESSDDEDD 180
           PRRGRRSEAAALEDYVRNTLEQTFASIRQQNAEVMENK NVFKEKVDNEVESESSDDE+D
Sbjct: 133 PRRGRRSEAAALEDYVRNTLEQTFASIRQQNAEVMENKGNVFKEKVDNEVESESSDDEED 192

Query: 181 ADEEEDVEQNDGAGRNKKKKMVVEEEDPNWPLDADVGWGVRASEYFEQHPIKNVVGDDGV 240
            DEEEDVEQNDGAGRNKKKKMVVEEEDPNWPLDADVGWGVRASEYFEQ+PIKNVVGDDGV
Sbjct: 193 EDEEEDVEQNDGAGRNKKKKMVVEEEDPNWPLDADVGWGVRASEYFEQYPIKNVVGDDGV 252

Query: 241 EIDWEGEIDDSWVQEINCLEWESFAFHPSPLVVLVFERYNRATDNWKTLKELETALKVYW 300
           +IDWEGEIDD+WVQEINCLEWESFAFHPSPLVVLVFERYNRATDNWKTLKELE AL+VYW
Sbjct: 253 KIDWEGEIDDNWVQEINCLEWESFAFHPSPLVVLVFERYNRATDNWKTLKELEKALQVYW 312

Query: 301 NAKDRLPPRSVKIDINIERDLAYALKVRECPQILFLRGNKVLYREKDFRTADELVQMIAF 360
           NAKDRLPPRSVKIDINIERDLAYALKVRECPQILFLRGNKVLYREKDFRTADELVQMIAF
Sbjct: 313 NAKDRLPPRSVKIDINIERDLAYALKVRECPQILFLRGNKVLYREKDFRTADELVQMIAF 372

Query: 361 FYYKAKKPSWINDKALARPF 381
           FYYKAKKP WINDKAL+RPF
Sbjct: 373 FYYKAKKPLWINDKALSRPF 392

BLAST of CSPI03G26650 vs. ExPASy TrEMBL
Match: A0A6J1HUF9 (thioredoxin-like fold domain-containing protein MRL7, chloroplastic OS=Cucurbita maxima OX=3661 GN=LOC111466968 PE=4 SV=1)

HSP 1 Score: 646.7 bits (1667), Expect = 5.9e-182
Identity = 333/384 (86.72%), Postives = 353/384 (91.93%), Query Frame = 0

Query: 1   MLILQASFISMTVSPQYVVHNAFTLRRFLTAPTEYQTFKPSCYFRYHSASILPNVVHHRN 60
           M+ LQA  ISM VSPQY V+NA TL RFLT P EYQTFKPSCY R+HS SILPN+VH  +
Sbjct: 1   MMFLQAPSISMPVSPQYTVYNASTLHRFLTVPIEYQTFKPSCYSRFHSVSILPNLVHFSH 60

Query: 61  SRKLPCLAVSSKSNSEHDPEPSADSKVKPKPISRIKRSKTSSEKEEENGAGIFPTTIPRK 120
           SRKLPCL VSSKS+SEHD +PSADSKVKPK  SR ++SK +SEKEEEN   IFPTTIPRK
Sbjct: 61  SRKLPCLVVSSKSSSEHDSQPSADSKVKPKSRSRSRKSK-ASEKEEENDDRIFPTTIPRK 120

Query: 121 PRRGRRSEAAALEDYVRNTLEQTFASIRQQNAEVMENKENVFKEKVDNEVESESSDDE-- 180
           PRRGRRSEAAALEDYVRNTLEQTFASIRQ+NAEVMENKENVFKEKVD+EV+ ESSDDE  
Sbjct: 121 PRRGRRSEAAALEDYVRNTLEQTFASIRQKNAEVMENKENVFKEKVDDEVDPESSDDEED 180

Query: 181 DDADEEEDVEQNDGAGRN--KKKKMVVEEEDPNWPLDADVGWGVRASEYFEQHPIKNVVG 240
           +D DEEE+VE++DG GRN  KKKK+VVEEEDPNWPLDADVGWGVRASEYFEQHPIKNVVG
Sbjct: 181 EDEDEEEEVEKSDGTGRNKKKKKKIVVEEEDPNWPLDADVGWGVRASEYFEQHPIKNVVG 240

Query: 241 DDGVEIDWEGEIDDSWVQEINCLEWESFAFHPSPLVVLVFERYNRATDNWKTLKELETAL 300
           DDGVEIDWEGEIDD+WVQEINCLEWESFAFHPSPLVVLVFERYNRATDNWKTLKELE AL
Sbjct: 241 DDGVEIDWEGEIDDNWVQEINCLEWESFAFHPSPLVVLVFERYNRATDNWKTLKELEKAL 300

Query: 301 KVYWNAKDRLPPRSVKIDINIERDLAYALKVRECPQILFLRGNKVLYREKDFRTADELVQ 360
           +VYWNAKDRLPPRSVKIDINIERDLAYALKVRECPQILFLRGNKVLYREKDFRTADELVQ
Sbjct: 301 QVYWNAKDRLPPRSVKIDINIERDLAYALKVRECPQILFLRGNKVLYREKDFRTADELVQ 360

Query: 361 MIAFFYYKAKKPSWINDKALARPF 381
           MIAFFYYKAKKP WINDKALARPF
Sbjct: 361 MIAFFYYKAKKPLWINDKALARPF 383

BLAST of CSPI03G26650 vs. ExPASy TrEMBL
Match: A0A6J1HJ24 (thioredoxin-like fold domain-containing protein MRL7, chloroplastic OS=Cucurbita moschata OX=3662 GN=LOC111464001 PE=4 SV=1)

HSP 1 Score: 641.7 bits (1654), Expect = 1.9e-180
Identity = 331/381 (86.88%), Postives = 351/381 (92.13%), Query Frame = 0

Query: 1   MLILQASFISMTVSPQYVVHNAFTLRRFLTAPTEYQTFKPSCYFRYHSASILPNVVHHRN 60
           M+ LQA  +SM VSPQY VH+A TL RFLT P EYQTFKPS Y R+HS SILPN+VH  +
Sbjct: 1   MMFLQAPSVSMPVSPQYTVHSA-TLHRFLTVPIEYQTFKPSRYSRFHSVSILPNLVHFSH 60

Query: 61  SRKLPCLAVSSKSNSEHDPEPSADSKVKPKPISRIKRSKTSSEKEEENGAGIFPTTIPRK 120
           SRKLPCL +SSKS+SE D +PSADSKVKPK  SR ++SK +SEKEEEN   IFPTTIPRK
Sbjct: 61  SRKLPCLVLSSKSSSEQDSQPSADSKVKPKSRSRSRKSK-ASEKEEENDDRIFPTTIPRK 120

Query: 121 PRRGRRSEAAALEDYVRNTLEQTFASIRQQNAEVMENKENVFKEKVDNEVESESSDDEDD 180
           PRRGRRSEAAALEDYVRNTLEQTFASIRQQNAEVMENKENVFKEKVD+EVESESSDDE+D
Sbjct: 121 PRRGRRSEAAALEDYVRNTLEQTFASIRQQNAEVMENKENVFKEKVDDEVESESSDDEED 180

Query: 181 ADEEEDVEQNDGAGRN-KKKKMVVEEEDPNWPLDADVGWGVRASEYFEQHPIKNVVGDDG 240
            DEEE+VE++DG GRN KKKK+VVEEEDPNWPLDADVGWGVRASEYFEQHPIKNVVGDDG
Sbjct: 181 EDEEEEVEKSDGTGRNKKKKKIVVEEEDPNWPLDADVGWGVRASEYFEQHPIKNVVGDDG 240

Query: 241 VEIDWEGEIDDSWVQEINCLEWESFAFHPSPLVVLVFERYNRATDNWKTLKELETALKVY 300
           VEIDWEGEIDD+WVQEINCLEWESFAFHPSPLVVLVFERYNRATDNWKTLKELE AL+VY
Sbjct: 241 VEIDWEGEIDDNWVQEINCLEWESFAFHPSPLVVLVFERYNRATDNWKTLKELEKALQVY 300

Query: 301 WNAKDRLPPRSVKIDINIERDLAYALKVRECPQILFLRGNKVLYREKDFRTADELVQMIA 360
           WNAKDRLPPRSVKIDINIERDLAYALKVRECPQILFLRGNKVLYREKDFR ADELVQMIA
Sbjct: 301 WNAKDRLPPRSVKIDINIERDLAYALKVRECPQILFLRGNKVLYREKDFRMADELVQMIA 360

Query: 361 FFYYKAKKPSWINDKALARPF 381
           FFYYKAKKP WINDKALARPF
Sbjct: 361 FFYYKAKKPLWINDKALARPF 379

BLAST of CSPI03G26650 vs. NCBI nr
Match: XP_031739020.1 (thioredoxin-like fold domain-containing protein MRL7, chloroplastic isoform X1 [Cucumis sativus] >XP_031739021.1 thioredoxin-like fold domain-containing protein MRL7, chloroplastic isoform X1 [Cucumis sativus] >XP_031739022.1 thioredoxin-like fold domain-containing protein MRL7, chloroplastic isoform X1 [Cucumis sativus] >KGN58168.1 hypothetical protein Csa_017532 [Cucumis sativus])

HSP 1 Score: 753.4 bits (1944), Expect = 9.2e-214
Identity = 380/380 (100.00%), Postives = 380/380 (100.00%), Query Frame = 0

Query: 1   MLILQASFISMTVSPQYVVHNAFTLRRFLTAPTEYQTFKPSCYFRYHSASILPNVVHHRN 60
           MLILQASFISMTVSPQYVVHNAFTLRRFLTAPTEYQTFKPSCYFRYHSASILPNVVHHRN
Sbjct: 1   MLILQASFISMTVSPQYVVHNAFTLRRFLTAPTEYQTFKPSCYFRYHSASILPNVVHHRN 60

Query: 61  SRKLPCLAVSSKSNSEHDPEPSADSKVKPKPISRIKRSKTSSEKEEENGAGIFPTTIPRK 120
           SRKLPCLAVSSKSNSEHDPEPSADSKVKPKPISRIKRSKTSSEKEEENGAGIFPTTIPRK
Sbjct: 61  SRKLPCLAVSSKSNSEHDPEPSADSKVKPKPISRIKRSKTSSEKEEENGAGIFPTTIPRK 120

Query: 121 PRRGRRSEAAALEDYVRNTLEQTFASIRQQNAEVMENKENVFKEKVDNEVESESSDDEDD 180
           PRRGRRSEAAALEDYVRNTLEQTFASIRQQNAEVMENKENVFKEKVDNEVESESSDDEDD
Sbjct: 121 PRRGRRSEAAALEDYVRNTLEQTFASIRQQNAEVMENKENVFKEKVDNEVESESSDDEDD 180

Query: 181 ADEEEDVEQNDGAGRNKKKKMVVEEEDPNWPLDADVGWGVRASEYFEQHPIKNVVGDDGV 240
           ADEEEDVEQNDGAGRNKKKKMVVEEEDPNWPLDADVGWGVRASEYFEQHPIKNVVGDDGV
Sbjct: 181 ADEEEDVEQNDGAGRNKKKKMVVEEEDPNWPLDADVGWGVRASEYFEQHPIKNVVGDDGV 240

Query: 241 EIDWEGEIDDSWVQEINCLEWESFAFHPSPLVVLVFERYNRATDNWKTLKELETALKVYW 300
           EIDWEGEIDDSWVQEINCLEWESFAFHPSPLVVLVFERYNRATDNWKTLKELETALKVYW
Sbjct: 241 EIDWEGEIDDSWVQEINCLEWESFAFHPSPLVVLVFERYNRATDNWKTLKELETALKVYW 300

Query: 301 NAKDRLPPRSVKIDINIERDLAYALKVRECPQILFLRGNKVLYREKDFRTADELVQMIAF 360
           NAKDRLPPRSVKIDINIERDLAYALKVRECPQILFLRGNKVLYREKDFRTADELVQMIAF
Sbjct: 301 NAKDRLPPRSVKIDINIERDLAYALKVRECPQILFLRGNKVLYREKDFRTADELVQMIAF 360

Query: 361 FYYKAKKPSWINDKALARPF 381
           FYYKAKKPSWINDKALARPF
Sbjct: 361 FYYKAKKPSWINDKALARPF 380

BLAST of CSPI03G26650 vs. NCBI nr
Match: XP_016900441.1 (PREDICTED: uncharacterized protein LOC103489851 [Cucumis melo])

HSP 1 Score: 716.1 bits (1847), Expect = 1.6e-202
Identity = 361/380 (95.00%), Postives = 369/380 (97.11%), Query Frame = 0

Query: 1   MLILQASFISMTVSPQYVVHNAFTLRRFLTAPTEYQTFKPSCYFRYHSASILPNVVHHRN 60
           MLILQASFISMTVSPQYV+HNA TLRRFLT PTEYQTFKPSCYFRYHSASILPN+VHHRN
Sbjct: 13  MLILQASFISMTVSPQYVLHNASTLRRFLTVPTEYQTFKPSCYFRYHSASILPNLVHHRN 72

Query: 61  SRKLPCLAVSSKSNSEHDPEPSADSKVKPKPISRIKRSKTSSEKEEENGAGIFPTTIPRK 120
           SRKLPC AVSSKSNSEHDPEPSADSKVKPK  SRIKRSK SS KEEENGAGIFPTTIPRK
Sbjct: 73  SRKLPCFAVSSKSNSEHDPEPSADSKVKPKSRSRIKRSKASSGKEEENGAGIFPTTIPRK 132

Query: 121 PRRGRRSEAAALEDYVRNTLEQTFASIRQQNAEVMENKENVFKEKVDNEVESESSDDEDD 180
           PRRGRRSEAAALEDYVRNTLEQTFASIRQQNAEVMENK NVFKEKVDNEVESESSDDE+D
Sbjct: 133 PRRGRRSEAAALEDYVRNTLEQTFASIRQQNAEVMENKGNVFKEKVDNEVESESSDDEED 192

Query: 181 ADEEEDVEQNDGAGRNKKKKMVVEEEDPNWPLDADVGWGVRASEYFEQHPIKNVVGDDGV 240
            DEEEDVEQNDGAGRNKKKKMVVEEEDPNWPLDADVGWGVRASEYFEQ+PIKNVVGDDGV
Sbjct: 193 EDEEEDVEQNDGAGRNKKKKMVVEEEDPNWPLDADVGWGVRASEYFEQYPIKNVVGDDGV 252

Query: 241 EIDWEGEIDDSWVQEINCLEWESFAFHPSPLVVLVFERYNRATDNWKTLKELETALKVYW 300
           +IDWEGEIDD+WVQEINCLEWESFAFHPSPLVVLVFERYNRATDNWKTLKELE AL+VYW
Sbjct: 253 KIDWEGEIDDNWVQEINCLEWESFAFHPSPLVVLVFERYNRATDNWKTLKELEKALQVYW 312

Query: 301 NAKDRLPPRSVKIDINIERDLAYALKVRECPQILFLRGNKVLYREKDFRTADELVQMIAF 360
           NAKDRLPPRSVKIDINIERDLAYALKVRECPQILFLRGNKVLYREKDFRTADELVQMIAF
Sbjct: 313 NAKDRLPPRSVKIDINIERDLAYALKVRECPQILFLRGNKVLYREKDFRTADELVQMIAF 372

Query: 361 FYYKAKKPSWINDKALARPF 381
           FYYKAKKP WINDKAL+RPF
Sbjct: 373 FYYKAKKPLWINDKALSRPF 392

BLAST of CSPI03G26650 vs. NCBI nr
Match: KAA0037984.1 (Polyadenylate-binding protein 2-binding protein 2 [Cucumis melo var. makuwa])

HSP 1 Score: 716.1 bits (1847), Expect = 1.6e-202
Identity = 361/380 (95.00%), Postives = 369/380 (97.11%), Query Frame = 0

Query: 1   MLILQASFISMTVSPQYVVHNAFTLRRFLTAPTEYQTFKPSCYFRYHSASILPNVVHHRN 60
           MLILQASFISMTVSPQYV+HNA TLRRFLT PTEYQTFKPSCYFRYHSASILPN+VHHRN
Sbjct: 67  MLILQASFISMTVSPQYVLHNASTLRRFLTVPTEYQTFKPSCYFRYHSASILPNLVHHRN 126

Query: 61  SRKLPCLAVSSKSNSEHDPEPSADSKVKPKPISRIKRSKTSSEKEEENGAGIFPTTIPRK 120
           SRKLPC AVSSKSNSEHDPEPSADSKVKPK  SRIKRSK SS KEEENGAGIFPTTIPRK
Sbjct: 127 SRKLPCFAVSSKSNSEHDPEPSADSKVKPKSRSRIKRSKASSGKEEENGAGIFPTTIPRK 186

Query: 121 PRRGRRSEAAALEDYVRNTLEQTFASIRQQNAEVMENKENVFKEKVDNEVESESSDDEDD 180
           PRRGRRSEAAALEDYVRNTLEQTFASIRQQNAEVMENK NVFKEKVDNEVESESSDDE+D
Sbjct: 187 PRRGRRSEAAALEDYVRNTLEQTFASIRQQNAEVMENKGNVFKEKVDNEVESESSDDEED 246

Query: 181 ADEEEDVEQNDGAGRNKKKKMVVEEEDPNWPLDADVGWGVRASEYFEQHPIKNVVGDDGV 240
            DEEEDVEQNDGAGRNKKKKMVVEEEDPNWPLDADVGWGVRASEYFEQ+PIKNVVGDDGV
Sbjct: 247 EDEEEDVEQNDGAGRNKKKKMVVEEEDPNWPLDADVGWGVRASEYFEQYPIKNVVGDDGV 306

Query: 241 EIDWEGEIDDSWVQEINCLEWESFAFHPSPLVVLVFERYNRATDNWKTLKELETALKVYW 300
           +IDWEGEIDD+WVQEINCLEWESFAFHPSPLVVLVFERYNRATDNWKTLKELE AL+VYW
Sbjct: 307 KIDWEGEIDDNWVQEINCLEWESFAFHPSPLVVLVFERYNRATDNWKTLKELEKALQVYW 366

Query: 301 NAKDRLPPRSVKIDINIERDLAYALKVRECPQILFLRGNKVLYREKDFRTADELVQMIAF 360
           NAKDRLPPRSVKIDINIERDLAYALKVRECPQILFLRGNKVLYREKDFRTADELVQMIAF
Sbjct: 367 NAKDRLPPRSVKIDINIERDLAYALKVRECPQILFLRGNKVLYREKDFRTADELVQMIAF 426

Query: 361 FYYKAKKPSWINDKALARPF 381
           FYYKAKKP WINDKAL+RPF
Sbjct: 427 FYYKAKKPLWINDKALSRPF 446

BLAST of CSPI03G26650 vs. NCBI nr
Match: XP_038888099.1 (thioredoxin-like fold domain-containing protein MRL7, chloroplastic [Benincasa hispida] >XP_038888100.1 thioredoxin-like fold domain-containing protein MRL7, chloroplastic [Benincasa hispida] >XP_038888101.1 thioredoxin-like fold domain-containing protein MRL7, chloroplastic [Benincasa hispida] >XP_038888102.1 thioredoxin-like fold domain-containing protein MRL7, chloroplastic [Benincasa hispida] >XP_038888103.1 thioredoxin-like fold domain-containing protein MRL7, chloroplastic [Benincasa hispida] >XP_038888104.1 thioredoxin-like fold domain-containing protein MRL7, chloroplastic [Benincasa hispida])

HSP 1 Score: 664.5 bits (1713), Expect = 5.6e-187
Identity = 338/380 (88.95%), Postives = 359/380 (94.47%), Query Frame = 0

Query: 1   MLILQASFISMTVSPQYVVHNAFTLRRFLTAPTEYQTFKPSCYFRYHSASILPNVVHHRN 60
           MLILQAS +SM+VSPQY VHNA TL+RFLT P E QTFKPSCY RYHS SILPN+VH+  
Sbjct: 1   MLILQASSLSMSVSPQYAVHNASTLQRFLTVPIECQTFKPSCYSRYHSHSILPNLVHYSY 60

Query: 61  SRKLPCLAVSSKSNSEHDPEPSADSKVKPKPISRIKRSKTSSEKEEENGAGIFPTTIPRK 120
           SRKLPCLAV SKSNSEHDPEP+ADSKVK KP SR KRSK + EKEE+N AGIFPTTIPRK
Sbjct: 61  SRKLPCLAVWSKSNSEHDPEPNADSKVKQKPRSRSKRSK-ALEKEEDNDAGIFPTTIPRK 120

Query: 121 PRRGRRSEAAALEDYVRNTLEQTFASIRQQNAEVMENKENVFKEKVDNEVESESSDDEDD 180
           PRRGRRSEAAALEDYVRNTLEQTFASIRQQNAEVMENKENV KEKVD++VE ESSDDE++
Sbjct: 121 PRRGRRSEAAALEDYVRNTLEQTFASIRQQNAEVMENKENVIKEKVDDKVEFESSDDEEE 180

Query: 181 ADEEEDVEQNDGAGRNKKKKMVVEEEDPNWPLDADVGWGVRASEYFEQHPIKNVVGDDGV 240
            DEEE++EQ+DGAGRNKKKKMVVEEEDPNWPLDADVGWGVRASEYFEQHPIKN+VGDDGV
Sbjct: 181 -DEEEEIEQSDGAGRNKKKKMVVEEEDPNWPLDADVGWGVRASEYFEQHPIKNIVGDDGV 240

Query: 241 EIDWEGEIDDSWVQEINCLEWESFAFHPSPLVVLVFERYNRATDNWKTLKELETALKVYW 300
           EIDWEGEI+D+WVQEINCLEWESFAFHPSPL+VLVFERYNRATDNWKTLKELE AL+VYW
Sbjct: 241 EIDWEGEIEDNWVQEINCLEWESFAFHPSPLIVLVFERYNRATDNWKTLKELEKALQVYW 300

Query: 301 NAKDRLPPRSVKIDINIERDLAYALKVRECPQILFLRGNKVLYREKDFRTADELVQMIAF 360
           NAKDRLPPRSVKIDINIERDLAYALKVRECPQILFLRGNKVLYREKDFRTADELVQMIAF
Sbjct: 301 NAKDRLPPRSVKIDINIERDLAYALKVRECPQILFLRGNKVLYREKDFRTADELVQMIAF 360

Query: 361 FYYKAKKPSWINDKALARPF 381
           FYYKAKKPSWINDKAL+RPF
Sbjct: 361 FYYKAKKPSWINDKALSRPF 378

BLAST of CSPI03G26650 vs. NCBI nr
Match: XP_022967425.1 (thioredoxin-like fold domain-containing protein MRL7, chloroplastic [Cucurbita maxima] >XP_022967426.1 thioredoxin-like fold domain-containing protein MRL7, chloroplastic [Cucurbita maxima])

HSP 1 Score: 646.7 bits (1667), Expect = 1.2e-181
Identity = 333/384 (86.72%), Postives = 353/384 (91.93%), Query Frame = 0

Query: 1   MLILQASFISMTVSPQYVVHNAFTLRRFLTAPTEYQTFKPSCYFRYHSASILPNVVHHRN 60
           M+ LQA  ISM VSPQY V+NA TL RFLT P EYQTFKPSCY R+HS SILPN+VH  +
Sbjct: 1   MMFLQAPSISMPVSPQYTVYNASTLHRFLTVPIEYQTFKPSCYSRFHSVSILPNLVHFSH 60

Query: 61  SRKLPCLAVSSKSNSEHDPEPSADSKVKPKPISRIKRSKTSSEKEEENGAGIFPTTIPRK 120
           SRKLPCL VSSKS+SEHD +PSADSKVKPK  SR ++SK +SEKEEEN   IFPTTIPRK
Sbjct: 61  SRKLPCLVVSSKSSSEHDSQPSADSKVKPKSRSRSRKSK-ASEKEEENDDRIFPTTIPRK 120

Query: 121 PRRGRRSEAAALEDYVRNTLEQTFASIRQQNAEVMENKENVFKEKVDNEVESESSDDE-- 180
           PRRGRRSEAAALEDYVRNTLEQTFASIRQ+NAEVMENKENVFKEKVD+EV+ ESSDDE  
Sbjct: 121 PRRGRRSEAAALEDYVRNTLEQTFASIRQKNAEVMENKENVFKEKVDDEVDPESSDDEED 180

Query: 181 DDADEEEDVEQNDGAGRN--KKKKMVVEEEDPNWPLDADVGWGVRASEYFEQHPIKNVVG 240
           +D DEEE+VE++DG GRN  KKKK+VVEEEDPNWPLDADVGWGVRASEYFEQHPIKNVVG
Sbjct: 181 EDEDEEEEVEKSDGTGRNKKKKKKIVVEEEDPNWPLDADVGWGVRASEYFEQHPIKNVVG 240

Query: 241 DDGVEIDWEGEIDDSWVQEINCLEWESFAFHPSPLVVLVFERYNRATDNWKTLKELETAL 300
           DDGVEIDWEGEIDD+WVQEINCLEWESFAFHPSPLVVLVFERYNRATDNWKTLKELE AL
Sbjct: 241 DDGVEIDWEGEIDDNWVQEINCLEWESFAFHPSPLVVLVFERYNRATDNWKTLKELEKAL 300

Query: 301 KVYWNAKDRLPPRSVKIDINIERDLAYALKVRECPQILFLRGNKVLYREKDFRTADELVQ 360
           +VYWNAKDRLPPRSVKIDINIERDLAYALKVRECPQILFLRGNKVLYREKDFRTADELVQ
Sbjct: 301 QVYWNAKDRLPPRSVKIDINIERDLAYALKVRECPQILFLRGNKVLYREKDFRTADELVQ 360

Query: 361 MIAFFYYKAKKPSWINDKALARPF 381
           MIAFFYYKAKKP WINDKALARPF
Sbjct: 361 MIAFFYYKAKKPLWINDKALARPF 383

BLAST of CSPI03G26650 vs. TAIR 10
Match: AT4G28590.1 (FUNCTIONS IN: molecular_function unknown; INVOLVED IN: biological_process unknown; LOCATED IN: chloroplast; EXPRESSED IN: 21 plant structures; EXPRESSED DURING: 10 growth stages; BEST Arabidopsis thaliana protein match is: Thioredoxin superfamily protein (TAIR:AT2G31840.1); Has 114 Blast hits to 112 proteins in 39 species: Archae - 2; Bacteria - 0; Metazoa - 17; Fungi - 6; Plants - 67; Viruses - 0; Other Eukaryotes - 22 (source: NCBI BLink). )

HSP 1 Score: 377.5 bits (968), Expect = 1.3e-104
Identity = 199/330 (60.30%), Postives = 245/330 (74.24%), Query Frame = 0

Query: 51  ILPNVVHHRNSRKLPCLAVSSKSNSEHDPEPSADSKVKPKPISRIKRSKTSSEKEEENGA 110
           + P  +  R+ ++   ++    S+S  DPE    SK + +   + K + T   + E N  
Sbjct: 34  VFPVTISSRSVKRFAAVS----SDSVLDPE----SKNQTRSRRKNKEAVTPIAETENNEK 93

Query: 111 GIFPTTIPRKPRRGRRSEAAALEDYVRNTLEQTFASIRQQNAEVMENKENVFKEKVDNEV 170
             FPT +PRK +RGRRSEA A+EDYVR++LE+TF++I++QN EV ENKE        N +
Sbjct: 94  --FPTKVPRKSKRGRRSEADAVEDYVRSSLERTFSTIKEQNPEVFENKEKA------NFI 153

Query: 171 ESESSDDEDDADEEEDVEQNDGAGRNKKKKMVVEEEDPNWPLDADVGWGVRASEYFEQHP 230
           +    D+E++ +EE                MVVEEEDP+WP+D DVGWG++ASEYF+ HP
Sbjct: 154 KDRGVDEEEEEEEE----------------MVVEEEDPDWPVDTDVGWGIKASEYFDTHP 213

Query: 231 IKNVVGDDGVEIDWEGEIDDSWVQEINCLEWESFAFHPSPLVVLVFERYNRATDNWKTLK 290
           IKNVVGDDG EIDWEGEIDDSWV+EINCLEWESFAFHPSPLVVLVFERY RA+DNWKTLK
Sbjct: 214 IKNVVGDDGSEIDWEGEIDDSWVKEINCLEWESFAFHPSPLVVLVFERYKRASDNWKTLK 273

Query: 291 ELETALKVYWNAKDRLPPRSVKIDINIERDLAYALKVRECPQILFLRGNKVLYREKDFRT 350
           ELE A+KVYW+AKDRLPPR+VKID+NIE DLAYALK +ECPQILFLRGN++LYREKDFRT
Sbjct: 274 ELEKAIKVYWDAKDRLPPRAVKIDLNIETDLAYALKAKECPQILFLRGNRILYREKDFRT 330

Query: 351 ADELVQMIAFFYYKAKKPSWINDKALARPF 381
           ADELV MIA FYYKAK+PS + DKA   P+
Sbjct: 334 ADELVHMIAHFYYKAKRPSCV-DKANVTPY 330

BLAST of CSPI03G26650 vs. TAIR 10
Match: AT2G31840.1 (Thioredoxin superfamily protein )

HSP 1 Score: 136.3 bits (342), Expect = 5.0e-32
Identity = 108/271 (39.85%), Postives = 145/271 (53.51%), Query Frame = 0

Query: 125 RRSEAAALEDYVRNTLEQTFASIRQQNAEVMENKENVFKEKVDNEVESESSDDEDDADEE 184
           ++ E +  ED      E+TF        + +E +E     K   EV  +  D E+  DEE
Sbjct: 78  KKEEDSDSEDEEDEVKEETFGGKEASLDDPVERRE---WRKTIREVIDKHPDIEE--DEE 137

Query: 185 EDVEQNDGAGRNKKKK------MVVEEEDPNWPLDADVGWGVRASEYFEQHPIKN----- 244
            D+ +     R K +K      +VV EEDPNWP DAD GWG   +++F +  IKN     
Sbjct: 138 IDMVEK----RRKMQKLLADYPLVVNEEDPNWPEDAD-GWGFSFNQFFNKITIKNEKKEE 197

Query: 245 ------VVGDDG-VEIDWEGEIDDSWVQEINCL---EWESFAFHP-SPLVVLVFERYNRA 304
                   GDD   EI W+   DD++++ I  L   EWE   F   SPL+VLV  RY R 
Sbjct: 198 EDDDEDSEGDDSEKEIVWQ---DDNYIRPIKDLTTAEWEEAVFKDISPLMVLVHNRYKRP 257

Query: 305 TDNWKTLKELETALKVYWNAKDRLP-PRSVKIDINIERDLAYALKVRECPQILFLRGNKV 364
            +N K  +ELE A++V WN    LP PR V +D  +E DL  ALKV   P+I+F +  K+
Sbjct: 258 KENEKFREELEKAIQVIWNC--GLPSPRCVAVDAVVETDLVSALKVSVFPEIIFTKAGKI 317

Query: 365 LYREKDFRTADELVQMIAFFYYKAKKPSWIN 373
           LYREK  RTADEL +++AFFYY A KP  +N
Sbjct: 318 LYREKGIRTADELSKIMAFFYYGAAKPPCLN 333

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
F4JLC11.8e-10360.30Thioredoxin-like fold domain-containing protein MRL7, chloroplastic OS=Arabidops... [more]
Q5QLS77.1e-10058.10Thioredoxin-like fold domain-containing protein MRL7 homolog, chloroplastic OS=O... [more]
Q9SKB67.0e-3139.85Thioredoxin-like fold domain-containing protein MRL7L, chloroplastic OS=Arabidop... [more]
Q0DI485.7e-2534.80Thioredoxin-like fold domain-containing protein MRL7L homolog, chloroplastic OS=... [more]
Match NameE-valueIdentityDescription
A0A0A0L8M24.5e-214100.00Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G576330 PE=4 SV=1[more]
A0A5A7T3E07.9e-20395.00Polyadenylate-binding protein 2-binding protein 2 OS=Cucumis melo var. makuwa OX... [more]
A0A1S4DWT57.9e-20395.00uncharacterized protein LOC103489851 OS=Cucumis melo OX=3656 GN=LOC103489851 PE=... [more]
A0A6J1HUF95.9e-18286.72thioredoxin-like fold domain-containing protein MRL7, chloroplastic OS=Cucurbita... [more]
A0A6J1HJ241.9e-18086.88thioredoxin-like fold domain-containing protein MRL7, chloroplastic OS=Cucurbita... [more]
Match NameE-valueIdentityDescription
XP_031739020.19.2e-214100.00thioredoxin-like fold domain-containing protein MRL7, chloroplastic isoform X1 [... [more]
XP_016900441.11.6e-20295.00PREDICTED: uncharacterized protein LOC103489851 [Cucumis melo][more]
KAA0037984.11.6e-20295.00Polyadenylate-binding protein 2-binding protein 2 [Cucumis melo var. makuwa][more]
XP_038888099.15.6e-18788.95thioredoxin-like fold domain-containing protein MRL7, chloroplastic [Benincasa h... [more]
XP_022967425.11.2e-18186.72thioredoxin-like fold domain-containing protein MRL7, chloroplastic [Cucurbita m... [more]
Match NameE-valueIdentityDescription
AT4G28590.11.3e-10460.30FUNCTIONS IN: molecular_function unknown; INVOLVED IN: biological_process unknow... [more]
AT2G31840.15.0e-3239.85Thioredoxin superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (PI 183967) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 137..157
NoneNo IPR availableGENE3D3.40.30.10Glutaredoxincoord: 244..369
e-value: 1.1E-6
score: 30.2
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 77..106
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 69..128
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 190..206
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 158..206
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 171..189
NoneNo IPR availablePANTHERPTHR34669:SF2THIOREDOXIN-LIKE FOLD DOMAIN-CONTAINING PROTEIN MRL7, CHLOROPLASTICcoord: 22..379
NoneNo IPR availableCDDcd02947TRX_familycoord: 311..359
e-value: 1.80905E-4
score: 38.3081
IPR044701Thioredoxin-like fold domain-containing protein MRL7/MRL7LPANTHERPTHR34669THIOREDOXIN-LIKE FOLD DOMAIN-CONTAINING PROTEIN MRL7L, CHLOROPLASTICcoord: 22..379
IPR036249Thioredoxin-like superfamilySUPERFAMILY52833Thioredoxin-likecoord: 253..359

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CSPI03G26650.1CSPI03G26650.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0009658 chloroplast organization
biological_process GO:0006355 regulation of transcription, DNA-templated