CSPI03G23140 (gene) Cucumber (PI 183967) v1

BLAST of CSPI03G23140 vs. ExPASy Swiss-Prot
Match: P0C2F6 (Putative ribonuclease H protein At1g65750 OS=Arabidopsis thaliana OX=3702 GN=At1g65750 PE=3 SV=1)

HSP 1 Score: 72.0 bits (175), Expect = 1.2e-11
Identity = 33/76 (43.42%), Postives = 45/76 (59.21%), Query Frame = 0

Query: 1   MPESALSSLERIMRKFFWKGNKVGKLNHLVKWDAVIKSYKDGGLSLGKLKHKNMALLSKW 60
           +P+S L+ L+++ R F W      K  HLVKW  V    K+GGL +   K  N AL+SK 
Sbjct: 58  LPQSILNRLDQLSRTFLWGSTAEKKKQHLVKWSKVCSPKKEGGLGVRAAKSMNRALISKV 117

Query: 61  GWRFSKEQNSLWCQVI 77
           GWR  +E+NSLW  V+
Sbjct: 118 GWRLLQEKNSLWTLVL 133

BLAST of CSPI03G23140 vs. ExPASy TrEMBL
Match: A0A5A7T2Y0 (zf-RVT domain-containing protein OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold403G00100 PE=4 SV=1)

HSP 1 Score: 189.9 bits (481), Expect = 1.5e-44
Identity = 110/274 (40.15%), Postives = 149/274 (54.38%), Query Frame = 0

Query: 13  MRKFFWKGNKVGKLNHLVKWDAVIKSYKDGGLSLGKLKHKNMALLSKWGWRFSKEQNSLW 72
           MR FF +G+   K+N LV W+ V    KDGGL LG +K  N ALL+KWGWR+SKE+++LW
Sbjct: 1   MRNFFREGHADSKINQLVNWNKVSSPPKDGGLGLGGIKIHNTALLAKWGWRYSKEESALW 60

Query: 73  CQVIISIHGSSTFNWHSNEKETH-----------------------------------PW 132
            QVI SIHG   F+W +  K  +                                   PW
Sbjct: 61  RQVIRSIHGKEAFDWFTKGKSGNSLRSPWVNIARFWRLVDSLASFNLGNGLRIGFRIDPW 120

Query: 133 MDIITLERKFPRLVRIALNPNGSVSNHWDSSTNSWSIYFRRSLNDEEILDFRTLLSQISP 192
           +    ++ +F RL RIAL P  S          SW + FRRSL DEEI +F++LL  +S 
Sbjct: 121 VGNTPIKEQFSRLFRIALWPRCSFLFF------SWPLAFRRSLEDEEITEFQSLLFLLST 180

Query: 193 SQSASFPDQRFWSLETSGSFLVKSLVKHLSTSSPLESFLYKRFWKSNSPRRINISIWIML 252
            +  +  D R WS+E+ G F  KSL  HL T+SP++  L+    +S SPRRINI IWIM+
Sbjct: 181 EKVVNSDDFRSWSIESHGRFSDKSLSTHLKTASPMDKRLFSAILQSGSPRRINILIWIMV 240

BLAST of CSPI03G23140 vs. ExPASy TrEMBL
Match: A0A5D3DE60 (zf-RVT domain-containing protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold494G00090 PE=4 SV=1)

HSP 1 Score: 186.4 bits (472), Expect = 1.6e-43
Identity = 109/274 (39.78%), Postives = 149/274 (54.38%), Query Frame = 0

Query: 13  MRKFFWKGNKVGKLNHLVKWDAVIKSYKDGGLSLGKLKHKNMALLSKWGWRFSKEQNSLW 72
           MR FF +G+   K+N LV W+ V    KDGGL LG +K  N ALL+KWGWR+SKE+++LW
Sbjct: 1   MRNFFREGHADSKINQLVNWNKVSSLPKDGGLGLGGIKIHNTALLAKWGWRYSKEESALW 60

Query: 73  CQVIISIHGSSTFNWHS-----NEKETH------------------------------PW 132
            QVI SIHG   F+W +     N   +H                              PW
Sbjct: 61  RQVIRSIHGKEAFDWFTKGKSGNSLRSHWVNIARFWRLVDSLASFNLGNGLRIGFRIDPW 120

Query: 133 MDIITLERKFPRLVRIALNPNGSVSNHWDSSTNSWSIYFRRSLNDEEILDFRTLLSQISP 192
           +    ++ +F RL  IAL P             SW + FRRSL DEEI +F++LL  +S 
Sbjct: 121 VGNTPIKEQFSRLFSIALWPRCYFLFF------SWPLAFRRSLEDEEITEFQSLLFLLST 180

Query: 193 SQSASFPDQRFWSLETSGSFLVKSLVKHLSTSSPLESFLYKRFWKSNSPRRINISIWIML 252
            +  +  D R WS+E+ G F  KSL  HL T+SP++  L+    +S SPRRINI IWIM+
Sbjct: 181 EKVVNSDDFRSWSIESHGRFSDKSLSTHLKTASPMDKRLFSAILQSGSPRRINILIWIMV 240

BLAST of CSPI03G23140 vs. ExPASy TrEMBL
Match: A0A5A7SP09 (Vacuolar fusion protein MON1 homolog OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold284G00570 PE=3 SV=1)

HSP 1 Score: 175.3 bits (443), Expect = 3.7e-40
Identity = 92/198 (46.46%), Postives = 116/198 (58.59%), Query Frame = 0

Query: 15  KFFWKGNKVGKLNHLVKWDAVIKSYKDGGLSLGKLKHKNMALLSKWGWRFSKEQNSLWCQ 74
           +FFW+GN   KLN+LVKW+ V KS  DG L L KLK +NMALL+KW WRF  E +SLWC+
Sbjct: 185 EFFWEGNIDSKLNNLVKWELVTKSQGDGELGLDKLKIRNMALLAKWRWRFLDEPSSLWCK 244

Query: 75  VIISIHGSSTFNWHSNEKET-----------------------------------HPWMD 134
           V+ SIH S TF WH++ KE+                                    PWM+
Sbjct: 245 VVRSIHDSDTFIWHTSGKESSSLRSPWISISRQQRKVEILAVFKVGKGDRILFWLDPWMN 304

Query: 135 IITLERKFPRLVRIALNPNGSVSNHWDSSTNSWSIYFRRSLNDEEILDFRTLLSQISPSQ 178
            I L  KFPRL RIAL P GSV+N+WDSS++SWSI FR  L +EEI DF+ LL  +   +
Sbjct: 305 SIALWIKFPRLYRIALPPKGSVTNYWDSSSSSWSIIFRCLLKEEEISDFQFLLESLDGRR 364

BLAST of CSPI03G23140 vs. ExPASy TrEMBL
Match: A0A438D291 (Kinesin-like protein KIN-14I OS=Vitis vinifera OX=29760 GN=KIN14I_1 PE=4 SV=1)

HSP 1 Score: 167.5 bits (423), Expect = 7.8e-38
Identity = 95/242 (39.26%), Postives = 135/242 (55.79%), Query Frame = 0

Query: 6   LSSLERIMRKFFWKGNKVGKLNHLVKWDAVIKSYKDGGLSLGKLKHKNMALLSKWGWRFS 65
           ++ +ER  R F W G   GK +HLV+W+AV K    GGL +GK+  +N ALL KW WRF 
Sbjct: 2   VAKVERKQRDFLWSGVGEGKKDHLVRWEAVCKPRVKGGLGIGKIPLRNRALLGKWLWRFP 61

Query: 66  KEQNSLWCQVIISIHGSSTFNWHSNEKETHPWMDIITLERKFPRLVRIALNPNGSVSNHW 125
           +E  SLW QVI+SI+G+ +  W +N  E   W D I L+ ++PRL R+ ++ N  +S+  
Sbjct: 62  RESTSLWHQVILSIYGTRSNGWDANTVEDLWWGDQI-LKDQYPRLYRVVMDKNIPISSIL 121

Query: 126 DS-STNSWSIYFRRSLNDEEILDFRTLLSQIS-PSQSASFPDQRFWSLETSGSFLVKSLV 185
            S    SW+  FRR+L D EI D   L+  +   + S S  D R WSL +SG F VKS  
Sbjct: 122 GSPHPFSWNFNFRRNLTDSEIEDLECLMHSLDCMNLSTSALDARSWSLLSSGLFTVKSFF 181

Query: 186 KHLSTSSPLESFLYKRF-WKSNSPRRINISIWIMLFENLDCASVLQRKLSSHALSPYVCP 245
             LS       F   +F WKS  P ++   +W++  + L+   +LQ +    A SP +C 
Sbjct: 182 TALSQMPDSSPFFPTKFVWKSQVPFKVKAFVWLVAHKKLNTNDLLQLRRPHKAFSPDICK 241

BLAST of CSPI03G23140 vs. ExPASy TrEMBL
Match: A0A438F983 (Putative ribonuclease H protein OS=Vitis vinifera OX=29760 GN=VvCHDh000004_319 PE=4 SV=1)

HSP 1 Score: 166.8 bits (421), Expect = 1.3e-37
Identity = 94/251 (37.45%), Postives = 141/251 (56.18%), Query Frame = 0

Query: 1   MPESALSSLERIMRKFFWKGNKVGKLNHLVKWDAVIKSYKDGGLSLGKLKHKNMALLSKW 60
           +P S  + +ER+ R F W G   GK +HLV+WD V K    GGL LG +  +N+ALL KW
Sbjct: 497 IPASMAAKIERLQRDFLWSGVGEGKRDHLVRWDVVCKPKTIGGLGLGNISWRNLALLGKW 556

Query: 61  GWRFSKEQNSLWCQVIISIHGSSTFNWHSNE--KETH--PWMDIITLERKFPRLVRIALN 120
            WR+ +E ++LW QVI+SI+GS +  W +N   + +H  PW  I  + + F  + R+ ++
Sbjct: 557 LWRYPREGSALWHQVILSIYGSHSNGWDANTLVRWSHRCPWKAIAQVFQGFSLITRVVMD 616

Query: 121 PNGSVSNHWDSSTN-SWSIYFRRSLNDEEILDFRTLLSQISPSQ-SASFPDQRFWSLETS 180
            N S+S+    S    W++ FRR+L+D EI D   L+  +     S S PD R W L +S
Sbjct: 617 KNISISSVLGPSRPFLWNLNFRRNLSDSEIEDLEGLMRSLDDLYLSPSVPDARLWPLSSS 676

Query: 181 GSFLVKSLVKHLSTSS-PLESFLYKRFWKSNSPRRINISIWIMLFENLDCASVLQRKLSS 240
           G F VKS    LS SS   ++F  K  W S  P ++   +W++  + ++   +LQ +   
Sbjct: 677 GLFSVKSFFLALSQSSGSPQNFPSKFVWNSQVPFKVKSFVWLVAHKKVNTNDMLQVRRPY 736

Query: 241 HALSPYVCPLC 245
            ALSP +C LC
Sbjct: 737 KALSPDICILC 747

BLAST of CSPI03G23140 vs. NCBI nr
Match: KAA0035739.1 (hypothetical protein E6C27_scaffold403G00100 [Cucumis melo var. makuwa])

HSP 1 Score: 189.9 bits (481), Expect = 3.0e-44
Identity = 110/274 (40.15%), Postives = 149/274 (54.38%), Query Frame = 0

Query: 13  MRKFFWKGNKVGKLNHLVKWDAVIKSYKDGGLSLGKLKHKNMALLSKWGWRFSKEQNSLW 72
           MR FF +G+   K+N LV W+ V    KDGGL LG +K  N ALL+KWGWR+SKE+++LW
Sbjct: 1   MRNFFREGHADSKINQLVNWNKVSSPPKDGGLGLGGIKIHNTALLAKWGWRYSKEESALW 60

Query: 73  CQVIISIHGSSTFNWHSNEKETH-----------------------------------PW 132
            QVI SIHG   F+W +  K  +                                   PW
Sbjct: 61  RQVIRSIHGKEAFDWFTKGKSGNSLRSPWVNIARFWRLVDSLASFNLGNGLRIGFRIDPW 120

Query: 133 MDIITLERKFPRLVRIALNPNGSVSNHWDSSTNSWSIYFRRSLNDEEILDFRTLLSQISP 192
           +    ++ +F RL RIAL P  S          SW + FRRSL DEEI +F++LL  +S 
Sbjct: 121 VGNTPIKEQFSRLFRIALWPRCSFLFF------SWPLAFRRSLEDEEITEFQSLLFLLST 180

Query: 193 SQSASFPDQRFWSLETSGSFLVKSLVKHLSTSSPLESFLYKRFWKSNSPRRINISIWIML 252
            +  +  D R WS+E+ G F  KSL  HL T+SP++  L+    +S SPRRINI IWIM+
Sbjct: 181 EKVVNSDDFRSWSIESHGRFSDKSLSTHLKTASPMDKRLFSAILQSGSPRRINILIWIMV 240

BLAST of CSPI03G23140 vs. NCBI nr
Match: TYK21876.1 (hypothetical protein E5676_scaffold494G00090 [Cucumis melo var. makuwa])

HSP 1 Score: 186.4 bits (472), Expect = 3.3e-43
Identity = 109/274 (39.78%), Postives = 149/274 (54.38%), Query Frame = 0

Query: 13  MRKFFWKGNKVGKLNHLVKWDAVIKSYKDGGLSLGKLKHKNMALLSKWGWRFSKEQNSLW 72
           MR FF +G+   K+N LV W+ V    KDGGL LG +K  N ALL+KWGWR+SKE+++LW
Sbjct: 1   MRNFFREGHADSKINQLVNWNKVSSLPKDGGLGLGGIKIHNTALLAKWGWRYSKEESALW 60

Query: 73  CQVIISIHGSSTFNWHS-----NEKETH------------------------------PW 132
            QVI SIHG   F+W +     N   +H                              PW
Sbjct: 61  RQVIRSIHGKEAFDWFTKGKSGNSLRSHWVNIARFWRLVDSLASFNLGNGLRIGFRIDPW 120

Query: 133 MDIITLERKFPRLVRIALNPNGSVSNHWDSSTNSWSIYFRRSLNDEEILDFRTLLSQISP 192
           +    ++ +F RL  IAL P             SW + FRRSL DEEI +F++LL  +S 
Sbjct: 121 VGNTPIKEQFSRLFSIALWPRCYFLFF------SWPLAFRRSLEDEEITEFQSLLFLLST 180

Query: 193 SQSASFPDQRFWSLETSGSFLVKSLVKHLSTSSPLESFLYKRFWKSNSPRRINISIWIML 252
            +  +  D R WS+E+ G F  KSL  HL T+SP++  L+    +S SPRRINI IWIM+
Sbjct: 181 EKVVNSDDFRSWSIESHGRFSDKSLSTHLKTASPMDKRLFSAILQSGSPRRINILIWIMV 240

BLAST of CSPI03G23140 vs. NCBI nr
Match: KAA0032570.1 (protein SAND [Cucumis melo var. makuwa] >TYK20915.1 protein SAND [Cucumis melo var. makuwa])

HSP 1 Score: 175.3 bits (443), Expect = 7.7e-40
Identity = 92/198 (46.46%), Postives = 116/198 (58.59%), Query Frame = 0

Query: 15  KFFWKGNKVGKLNHLVKWDAVIKSYKDGGLSLGKLKHKNMALLSKWGWRFSKEQNSLWCQ 74
           +FFW+GN   KLN+LVKW+ V KS  DG L L KLK +NMALL+KW WRF  E +SLWC+
Sbjct: 185 EFFWEGNIDSKLNNLVKWELVTKSQGDGELGLDKLKIRNMALLAKWRWRFLDEPSSLWCK 244

Query: 75  VIISIHGSSTFNWHSNEKET-----------------------------------HPWMD 134
           V+ SIH S TF WH++ KE+                                    PWM+
Sbjct: 245 VVRSIHDSDTFIWHTSGKESSSLRSPWISISRQQRKVEILAVFKVGKGDRILFWLDPWMN 304

Query: 135 IITLERKFPRLVRIALNPNGSVSNHWDSSTNSWSIYFRRSLNDEEILDFRTLLSQISPSQ 178
            I L  KFPRL RIAL P GSV+N+WDSS++SWSI FR  L +EEI DF+ LL  +   +
Sbjct: 305 SIALWIKFPRLYRIALPPKGSVTNYWDSSSSSWSIIFRCLLKEEEISDFQFLLESLDGRR 364

BLAST of CSPI03G23140 vs. NCBI nr
Match: RVW29541.1 (Kinesin-like protein KIN-14I [Vitis vinifera])

HSP 1 Score: 167.5 bits (423), Expect = 1.6e-37
Identity = 95/242 (39.26%), Postives = 135/242 (55.79%), Query Frame = 0

Query: 6   LSSLERIMRKFFWKGNKVGKLNHLVKWDAVIKSYKDGGLSLGKLKHKNMALLSKWGWRFS 65
           ++ +ER  R F W G   GK +HLV+W+AV K    GGL +GK+  +N ALL KW WRF 
Sbjct: 2   VAKVERKQRDFLWSGVGEGKKDHLVRWEAVCKPRVKGGLGIGKIPLRNRALLGKWLWRFP 61

Query: 66  KEQNSLWCQVIISIHGSSTFNWHSNEKETHPWMDIITLERKFPRLVRIALNPNGSVSNHW 125
           +E  SLW QVI+SI+G+ +  W +N  E   W D I L+ ++PRL R+ ++ N  +S+  
Sbjct: 62  RESTSLWHQVILSIYGTRSNGWDANTVEDLWWGDQI-LKDQYPRLYRVVMDKNIPISSIL 121

Query: 126 DS-STNSWSIYFRRSLNDEEILDFRTLLSQIS-PSQSASFPDQRFWSLETSGSFLVKSLV 185
            S    SW+  FRR+L D EI D   L+  +   + S S  D R WSL +SG F VKS  
Sbjct: 122 GSPHPFSWNFNFRRNLTDSEIEDLECLMHSLDCMNLSTSALDARSWSLLSSGLFTVKSFF 181

Query: 186 KHLSTSSPLESFLYKRF-WKSNSPRRINISIWIMLFENLDCASVLQRKLSSHALSPYVCP 245
             LS       F   +F WKS  P ++   +W++  + L+   +LQ +    A SP +C 
Sbjct: 182 TALSQMPDSSPFFPTKFVWKSQVPFKVKAFVWLVAHKKLNTNDLLQLRRPHKAFSPDICK 241

BLAST of CSPI03G23140 vs. NCBI nr
Match: RVW56511.1 (putative ribonuclease H protein [Vitis vinifera])

HSP 1 Score: 166.8 bits (421), Expect = 2.7e-37
Identity = 94/251 (37.45%), Postives = 141/251 (56.18%), Query Frame = 0

Query: 1   MPESALSSLERIMRKFFWKGNKVGKLNHLVKWDAVIKSYKDGGLSLGKLKHKNMALLSKW 60
           +P S  + +ER+ R F W G   GK +HLV+WD V K    GGL LG +  +N+ALL KW
Sbjct: 497 IPASMAAKIERLQRDFLWSGVGEGKRDHLVRWDVVCKPKTIGGLGLGNISWRNLALLGKW 556

Query: 61  GWRFSKEQNSLWCQVIISIHGSSTFNWHSNE--KETH--PWMDIITLERKFPRLVRIALN 120
            WR+ +E ++LW QVI+SI+GS +  W +N   + +H  PW  I  + + F  + R+ ++
Sbjct: 557 LWRYPREGSALWHQVILSIYGSHSNGWDANTLVRWSHRCPWKAIAQVFQGFSLITRVVMD 616

Query: 121 PNGSVSNHWDSSTN-SWSIYFRRSLNDEEILDFRTLLSQISPSQ-SASFPDQRFWSLETS 180
            N S+S+    S    W++ FRR+L+D EI D   L+  +     S S PD R W L +S
Sbjct: 617 KNISISSVLGPSRPFLWNLNFRRNLSDSEIEDLEGLMRSLDDLYLSPSVPDARLWPLSSS 676

Query: 181 GSFLVKSLVKHLSTSS-PLESFLYKRFWKSNSPRRINISIWIMLFENLDCASVLQRKLSS 240
           G F VKS    LS SS   ++F  K  W S  P ++   +W++  + ++   +LQ +   
Sbjct: 677 GLFSVKSFFLALSQSSGSPQNFPSKFVWNSQVPFKVKSFVWLVAHKKVNTNDMLQVRRPY 736

Query: 241 HALSPYVCPLC 245
            ALSP +C LC
Sbjct: 737 KALSPDICILC 747

BLAST of CSPI03G23140 vs. TAIR 10
Match: AT4G29090.1 (Ribonuclease H-like superfamily protein )

HSP 1 Score: 58.5 bits (140), Expect = 9.8e-09
Identity = 64/291 (21.99%), Postives = 110/291 (37.80%), Query Frame = 0

Query: 1   MPESALSSLERIMRKFFWKGNKVGKLNHLVKWDAVIKSYKDGGLSLGKLKHKNMALLSKW 60
           +P++    +  ++  F+W+  +  K  H   WD +     +GG+    ++  N+ALL K 
Sbjct: 13  LPKTVCKQIISVLADFWWRNKQEAKGMHWKAWDHLSCYKAEGGIGFKDIEAFNLALLGKQ 72

Query: 61  GWRFSKEQNSLWCQVIIS--IHGSSTFNWHSNEKETHPWMDIITLERKFPRLVRIALNPN 120
            WR      SL  +V  S   H S   N     + +  W  I   +    +  R A+  N
Sbjct: 73  MWRMLSRPESLMAKVFKSRYFHKSDPLNAPLGSRPSFVWKSIHASQEILRQGAR-AVVGN 132

Query: 121 GSVSNHW-----DSSTNSWSIYFRR----------------SLNDE-------EILDF-- 180
           G     W     DS   S ++  +R                 L DE       ++++   
Sbjct: 133 GEDIIIWRHKWLDSKPASAALRMQRVPPQEYASVSSILKVSDLIDESGREWRKDVIEMLF 192

Query: 181 ----RTLLSQISPSQSASFPDQRFWSLETSGSFLVKS-------LVKHLST----SSPLE 240
               R L+ ++ P       D   W   +SG + VKS       ++   S+    S P  
Sbjct: 193 PEVERKLIGELRPG-GRRILDSYTWDYTSSGDYTVKSGYWVLTQIINKRSSPQEVSEPSL 252

Query: 241 SFLYKRFWKSNSPRRINISIWIMLFENLDCASVLQRKLSSHALSPYVCPLC 245
           + +Y++ WKS +  +I   +W  L  +L  A  L  +  S   +   CP C
Sbjct: 253 NPIYQKIWKSQTSPKIQHFLWKCLSNSLPVAGALAYRHLSKESACIRCPSC 301