CSPI03G21370 (gene) Cucumber (PI 183967) v1

Overview


Name	CSPI03G21370
Type	gene
Organism	Cucumis sativus var. hardwickii cv. PI 183967 (Cucumber (PI 183967) v1)
Description	zf-RVT domain-containing protein
Location	Chr3: 17608538 .. 17609008 (+)
RNA-Seq Expression	CSPI03G21370
Synteny	CSPI03G21370

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGCTTTGGGCCTTCTACATTGCTCCTCAATTATGCAAAGAAAGATCCCCAGCAGTTGTCTTTTGCCCTCGGTGTGCCCGTTTTGTATGAAGGACAGTGAGGACCTGCTGCATCTCTTTTTTATCTGTCCTTATTCAGCCAATTGCTGGGGGAATATGCTCTTCCTTTTCAATGTGGCATGGGCTTTTGATGGATCATTAAGCTCGAAAGTCTTTCAACTGCTTAGGCGCCCGTTACTGTCAAAGAGACCGCAAATAAATTGGGAAAATATGTCGAAAGCAATGCTTGTGGAACTCTGGTTTGAACGTAATCAACGAATCTTTCATAACAAGGCAAGGGGCTGGTTTGAAACTTCGGACACGGCAAAAAGAAATGCAGCATCTTGGTGTTCTTTGAACAAGGAATATAATGAGTATTCCATTCAAGATTTATGCTTAAACTGGGCCGCTTTCATCTCTCAACCCATTTAA

mRNA sequence

ATGGCTTTGGGCCTTCTACATTGCTCCTCAATTATGCAAAGAAAGATCCCCAGCAGTTGTCTTTTGCCCTCGGTGTGCCCGTTTTGTATGAAGGACAGTGAGGACCTGCTGCATCTCTTTTTTATCTGTCCTTATTCAGCCAATTGCTGGGGGAATATGCTCTTCCTTTTCAATGTGGCATGGGCTTTTGATGGATCATTAAGCTCGAAAGTCTTTCAACTGCTTAGGCGCCCGTTACTGTCAAAGAGACCGCAAATAAATTGGGAAAATATGTCGAAAGCAATGCTTGTGGAACTCTGGTTTGAACGTAATCAACGAATCTTTCATAACAAGGCAAGGGGCTGGTTTGAAACTTCGGACACGGCAAAAAGAAATGCAGCATCTTGGTGTTCTTTGAACAAGGAATATAATGAGTATTCCATTCAAGATTTATGCTTAAACTGGGCCGCTTTCATCTCTCAACCCATTTAA

Coding sequence (CDS)

ATGGCTTTGGGCCTTCTACATTGCTCCTCAATTATGCAAAGAAAGATCCCCAGCAGTTGTCTTTTGCCCTCGGTGTGCCCGTTTTGTATGAAGGACAGTGAGGACCTGCTGCATCTCTTTTTTATCTGTCCTTATTCAGCCAATTGCTGGGGGAATATGCTCTTCCTTTTCAATGTGGCATGGGCTTTTGATGGATCATTAAGCTCGAAAGTCTTTCAACTGCTTAGGCGCCCGTTACTGTCAAAGAGACCGCAAATAAATTGGGAAAATATGTCGAAAGCAATGCTTGTGGAACTCTGGTTTGAACGTAATCAACGAATCTTTCATAACAAGGCAAGGGGCTGGTTTGAAACTTCGGACACGGCAAAAAGAAATGCAGCATCTTGGTGTTCTTTGAACAAGGAATATAATGAGTATTCCATTCAAGATTTATGCTTAAACTGGGCCGCTTTCATCTCTCAACCCATTTAA

Protein sequence

MALGLLHCSSIMQRKIPSSCLLPSVCPFCMKDSEDLLHLFFICPYSANCWGNMLFLFNVAWAFDGSLSSKVFQLLRRPLLSKRPQINWENMSKAMLVELWFERNQRIFHNKARGWFETSDTAKRNAASWCSLNKEYNEYSIQDLCLNWAAFISQPI*

Homology

[Close]

BLAST of CSPI03G21370 vs. ExPASy TrEMBL
Match: A0A5A7V5N8 (GPI-anchor transamidase isoform X1 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold79G00520 PE=3 SV=1)

HSP 1 Score: 176.4 bits (446), Expect = 9.3e-41
Identity = 82/146 (56.16%), Postives = 104/146 (71.23%), Query Frame = 0


Query: 9   SSIMQRKIPSSCLLPSVCPFCMKDSEDLLHLFFICPYSANCWGNMLFLFNVAWAFDGSLS 68
           S +MQR++ +SCL+PS C  C+++ EDL  L F CPYS  CW N+L LF V WAFDGS +
Sbjct: 556 SLVMQRELLNSCLMPSACSLCLEEGEDLQPL-FTCPYSVKCWENLLSLFGVDWAFDGSFN 615

Query: 69  SKVFQLLRRPLLSKRPQINWENMSKAMLVELWFERNQRIFHNKARGWFETSDTAKRNAAS 128
           S + Q+L    L + P++ W NMSKA+L ++WFE NQRIF  K   W E  D AKRNAA+
Sbjct: 616 SNIKQILLGSSLKEGPRLIWGNMSKALLSDIWFECNQRIFRGKVMHWSERLDIAKRNAAT 675

Query: 129 WCSLNKEYNEYSIQDLCLNWAAFISQ 155
           WC LNKE+ +YSIQDL +NW AFISQ
Sbjct: 676 WCMLNKEFIDYSIQDLSVNWLAFISQ 700

[Close]

BLAST of CSPI03G21370 vs. ExPASy TrEMBL
Match: A0A5D3DE60 (zf-RVT domain-containing protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold494G00090 PE=4 SV=1)

HSP 1 Score: 163.3 bits (412), Expect = 8.2e-37
Identity = 78/154 (50.65%), Postives = 104/154 (67.53%), Query Frame = 0


Query: 1   MALGLLHCSSIMQRKIPSSCLLPSVCPFCMKDSEDLLHLFFICPYSANCWGNMLFLFNVA 60
           M    ++ S I+Q+K P + + PS+CP C+K S++L H+F  CP S+  W  +  LFN+ 
Sbjct: 233 MVFRFVNSSEILQKKSPIN-VSPSICPLCLKASKNLPHIFLYCPVSSFGWERIFSLFNLV 292

Query: 61  WAFDGSLSSKVFQLLRRPLLSKRPQINWENMSKAMLVELWFERNQRIFHNKARGWFETSD 120
           W FD SLS+ V QLL    L K P+I WE +SKA+L+E+W ERNQRIFH+KAR   E   
Sbjct: 293 WNFDSSLSASVIQLLSGSNLPKTPRIIWEILSKALLIEIWIERNQRIFHDKARQRAEIMH 352

Query: 121 TAKRNAASWCSLNKEYNEYSIQDLCLNWAAFISQ 155
            A  NAA+WCSL KE+  YSIQD+CLNW  F++Q
Sbjct: 353 AADLNAAAWCSLRKEFVNYSIQDICLNWNVFLNQ 385

[Close]

BLAST of CSPI03G21370 vs. ExPASy TrEMBL
Match: A0A5A7T2Y0 (zf-RVT domain-containing protein OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold403G00100 PE=4 SV=1)

HSP 1 Score: 158.3 bits (399), Expect = 2.6e-35
Identity = 76/142 (53.52%), Postives = 98/142 (69.01%), Query Frame = 0


Query: 9   SSIMQRKIPSSCLLPSVCPFCMKDSEDLLHLFFICPYSANCWGNMLFLFNVAWAFDGSLS 68
           S I+Q+K P + + PS+CP C+K S++L H+F  CP S+  W  +  LFN+ W FD SLS
Sbjct: 241 SEILQKKSPIN-VSPSICPLCLKASKNLPHIFLYCPVSSFGWERIFSLFNLVWNFDSSLS 300

Query: 69  SKVFQLLRRPLLSKRPQINWENMSKAMLVELWFERNQRIFHNKARGWFETSDTAKRNAAS 128
           + V QLL    L K P+I WE +SKA+L+E+W ERNQRIFH+KAR   E    A  NAA+
Sbjct: 301 ASVIQLLSGSNLPKTPRIIWEILSKALLIEIWIERNQRIFHDKARQRAEIMHAADLNAAA 360

Query: 129 WCSLNKEYNEYSIQDLCLNWAA 151
           WCSL KE+  YSIQD+CLNW A
Sbjct: 361 WCSLRKEFVNYSIQDICLNWNA 381

[Close]

BLAST of CSPI03G21370 vs. ExPASy TrEMBL
Match: A0A6J1DIE2 (uncharacterized protein LOC111020765 OS=Momordica charantia OX=3673 GN=LOC111020765 PE=4 SV=1)

HSP 1 Score: 126.3 bits (316), Expect = 1.1e-25
Identity = 66/151 (43.71%), Postives = 87/151 (57.62%), Query Frame = 0


Query: 4   GLLHCSSIMQRKIPSSCLLPSVCPFCMKDSEDLLHLFFICPYSANCWGNMLFLFNVAWAF 63
           G L+ + I+Q+K PS  LLPS C  C K  ED  HLFF C +++ CW  +   FNV W F
Sbjct: 51  GKLNTADIIQKKSPSDALLPSFCCLCTKSGEDHDHLFFHCYFASKCWNLLFHQFNVDWCF 110

Query: 64  DGSLSSKVFQLLR-RPLLSKRPQINWENMSKAMLVELWFERNQRIFHNKARGWFETSDTA 123
           D      V+QLL   P LS   +  W N+ KA+L ELWFERN R+F  K R + E+  +A
Sbjct: 111 DLKAGDNVYQLLHGPPHLSSSVRFLWLNVVKALLSELWFERNSRLFEEKRRLFDESFYSA 170

Query: 124 KRNAASWCSLNKEYNEYSIQDLCLNWAAFIS 154
           K  A+ WCSL   +  +S   +  NW AFI+
Sbjct: 171 KFKASLWCSLVDSFLHHSPSMIYANWGAFIN 201

[Close]

BLAST of CSPI03G21370 vs. ExPASy TrEMBL
Match: A0A5D3DC06 (Mediator of RNA polymerase II transcription subunit 8 isoform X2 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold392G00890 PE=4 SV=1)

HSP 1 Score: 108.2 bits (269), Expect = 3.1e-20
Identity = 58/142 (40.85%), Postives = 75/142 (52.82%), Query Frame = 0


Query: 8   CSSIMQRKIPSSCLLPSVCPFCMKDSEDLLHLFFICPYSANCWGNMLFLFNVAWAFDGSL 67
           C+ I+Q+K P+ CLLPSVCP C K                                    
Sbjct: 144 CADILQKKAPNKCLLPSVCPLCQK------------------------------------ 203

Query: 68  SSKVFQLLRRPLLSKRPQINWENMSKAMLVELWFERNQRIFHNKARGWFETSDTAKRNA- 127
                 L   P L K+P++ W N+SKA+L ELWFERNQ IFH K +   E   +AK+NA 
Sbjct: 204 ------LPEGPPLPKKPRLIWLNLSKALLSELWFERNQHIFHGKEKPRLEILLSAKQNAV 243

Query: 128 ASWCSLNKEYNEYSIQDLCLNW 149
            +WCSLNKE+ +YSIQ++CLNW
Sbjct: 264 VAWCSLNKEFGDYSIQNICLNW 243

[Close]

BLAST of CSPI03G21370 vs. NCBI nr
Match: KAA0062564.1 (GPI-anchor transamidase isoform X1 [Cucumis melo var. makuwa])

HSP 1 Score: 176.4 bits (446), Expect = 1.9e-40
Identity = 82/146 (56.16%), Postives = 104/146 (71.23%), Query Frame = 0


Query: 9   SSIMQRKIPSSCLLPSVCPFCMKDSEDLLHLFFICPYSANCWGNMLFLFNVAWAFDGSLS 68
           S +MQR++ +SCL+PS C  C+++ EDL  L F CPYS  CW N+L LF V WAFDGS +
Sbjct: 556 SLVMQRELLNSCLMPSACSLCLEEGEDLQPL-FTCPYSVKCWENLLSLFGVDWAFDGSFN 615

Query: 69  SKVFQLLRRPLLSKRPQINWENMSKAMLVELWFERNQRIFHNKARGWFETSDTAKRNAAS 128
           S + Q+L    L + P++ W NMSKA+L ++WFE NQRIF  K   W E  D AKRNAA+
Sbjct: 616 SNIKQILLGSSLKEGPRLIWGNMSKALLSDIWFECNQRIFRGKVMHWSERLDIAKRNAAT 675

Query: 129 WCSLNKEYNEYSIQDLCLNWAAFISQ 155
           WC LNKE+ +YSIQDL +NW AFISQ
Sbjct: 676 WCMLNKEFIDYSIQDLSVNWLAFISQ 700

[Close]

BLAST of CSPI03G21370 vs. NCBI nr
Match: TYK21876.1 (hypothetical protein E5676_scaffold494G00090 [Cucumis melo var. makuwa])

HSP 1 Score: 163.3 bits (412), Expect = 1.7e-36
Identity = 78/154 (50.65%), Postives = 104/154 (67.53%), Query Frame = 0


Query: 1   MALGLLHCSSIMQRKIPSSCLLPSVCPFCMKDSEDLLHLFFICPYSANCWGNMLFLFNVA 60
           M    ++ S I+Q+K P + + PS+CP C+K S++L H+F  CP S+  W  +  LFN+ 
Sbjct: 233 MVFRFVNSSEILQKKSPIN-VSPSICPLCLKASKNLPHIFLYCPVSSFGWERIFSLFNLV 292

Query: 61  WAFDGSLSSKVFQLLRRPLLSKRPQINWENMSKAMLVELWFERNQRIFHNKARGWFETSD 120
           W FD SLS+ V QLL    L K P+I WE +SKA+L+E+W ERNQRIFH+KAR   E   
Sbjct: 293 WNFDSSLSASVIQLLSGSNLPKTPRIIWEILSKALLIEIWIERNQRIFHDKARQRAEIMH 352

Query: 121 TAKRNAASWCSLNKEYNEYSIQDLCLNWAAFISQ 155
            A  NAA+WCSL KE+  YSIQD+CLNW  F++Q
Sbjct: 353 AADLNAAAWCSLRKEFVNYSIQDICLNWNVFLNQ 385

[Close]

BLAST of CSPI03G21370 vs. NCBI nr
Match: KAA0035739.1 (hypothetical protein E6C27_scaffold403G00100 [Cucumis melo var. makuwa])

HSP 1 Score: 158.3 bits (399), Expect = 5.4e-35
Identity = 76/142 (53.52%), Postives = 98/142 (69.01%), Query Frame = 0


Query: 9   SSIMQRKIPSSCLLPSVCPFCMKDSEDLLHLFFICPYSANCWGNMLFLFNVAWAFDGSLS 68
           S I+Q+K P + + PS+CP C+K S++L H+F  CP S+  W  +  LFN+ W FD SLS
Sbjct: 241 SEILQKKSPIN-VSPSICPLCLKASKNLPHIFLYCPVSSFGWERIFSLFNLVWNFDSSLS 300

Query: 69  SKVFQLLRRPLLSKRPQINWENMSKAMLVELWFERNQRIFHNKARGWFETSDTAKRNAAS 128
           + V QLL    L K P+I WE +SKA+L+E+W ERNQRIFH+KAR   E    A  NAA+
Sbjct: 301 ASVIQLLSGSNLPKTPRIIWEILSKALLIEIWIERNQRIFHDKARQRAEIMHAADLNAAA 360

Query: 129 WCSLNKEYNEYSIQDLCLNWAA 151
           WCSL KE+  YSIQD+CLNW A
Sbjct: 361 WCSLRKEFVNYSIQDICLNWNA 381

[Close]

BLAST of CSPI03G21370 vs. NCBI nr
Match: XP_038903695.1 (uncharacterized protein LOC120090219 [Benincasa hispida])

HSP 1 Score: 157.5 bits (397), Expect = 9.2e-35
Identity = 74/155 (47.74%), Postives = 97/155 (62.58%), Query Frame = 0


Query: 1   MALGLLHCSSIMQRKIPSSCLLPSVCPFCMKDSEDLLHLFFICPYSANCWGNMLFLFNVA 60
           M  G L+ + ++Q+K P+  L P+VCPFC+  SE  LHLFF CPYS+ CW  +L  FN+ 
Sbjct: 163 MLFGQLNVAEVLQKKQPTPSLSPTVCPFCLHHSEVSLHLFFTCPYSSWCWNKLLCFFNLP 222

Query: 61  WAFDGSLSSKVFQLLRRPLLSKRPQINWENMSKAMLVELWFERNQRIFHNKARGWFETSD 120
                   S VFQLL RP   K  ++ W N  KA+L +LWFERNQRIF+NKA    +  +
Sbjct: 223 LTLCNDFKSNVFQLLARPTSHKSTRLLWCNAVKALLADLWFERNQRIFYNKATSCQDRLE 282

Query: 121 TAKRNAASWCSLNKEYNEYSIQDLCLNWAAFISQP 156
            A+R A+SWC L+  +  YS+ D  LNW AFIS P
Sbjct: 283 AARRQASSWCLLSDPFRAYSLSDFNLNWEAFISTP 317

[Close]

BLAST of CSPI03G21370 vs. NCBI nr
Match: XP_022153214.1 (uncharacterized protein LOC111020765 [Momordica charantia])

HSP 1 Score: 126.3 bits (316), Expect = 2.3e-25
Identity = 66/151 (43.71%), Postives = 87/151 (57.62%), Query Frame = 0


Query: 4   GLLHCSSIMQRKIPSSCLLPSVCPFCMKDSEDLLHLFFICPYSANCWGNMLFLFNVAWAF 63
           G L+ + I+Q+K PS  LLPS C  C K  ED  HLFF C +++ CW  +   FNV W F
Sbjct: 51  GKLNTADIIQKKSPSDALLPSFCCLCTKSGEDHDHLFFHCYFASKCWNLLFHQFNVDWCF 110

Query: 64  DGSLSSKVFQLLR-RPLLSKRPQINWENMSKAMLVELWFERNQRIFHNKARGWFETSDTA 123
           D      V+QLL   P LS   +  W N+ KA+L ELWFERN R+F  K R + E+  +A
Sbjct: 111 DLKAGDNVYQLLHGPPHLSSSVRFLWLNVVKALLSELWFERNSRLFEEKRRLFDESFYSA 170

Query: 124 KRNAASWCSLNKEYNEYSIQDLCLNWAAFIS 154
           K  A+ WCSL   +  +S   +  NW AFI+
Sbjct: 171 KFKASLWCSLVDSFLHHSPSMIYANWGAFIN 201

[Close]

BLAST of CSPI03G21370 vs. TAIR 10
Match: AT3G25270.1 (Ribonuclease H-like superfamily protein )

HSP 1 Score: 47.4 bits (111), Expect = 1.3e-05
Identity = 30/108 (27.78%), Postives = 48/108 (44.44%), Query Frame = 0


Query: 26  CPFCMKDSEDLLHLFFICPYSANCWGNMLFLFNVAWAFDGSLSSKVFQLLRRPLLSKRPQ 85
           C  C ++ E   HLFF C Y+   W               ++ +K+  LL   L +++PQ
Sbjct: 56  CHRCCQEDETSQHLFFDCFYAQQVWRASGIPHQELRTTGITMETKMELLLSSCLANRQPQ 115

Query: 86  INWENMSKAMLVELWFERNQRIFHNKARGWFETSDTAKRNAASWCSLN 134
           +   N++  +L  LW  RNQ +F  K+  W  T   A+ +   W   N
Sbjct: 116 L--FNLAIWILWRLWKSRNQLVFQQKSISWQNTLQRARNDVQEWEDTN 161

The following BLAST results are available for this feature:

BLAST of CSPI03G21370 vs.


Match Name	E-value	Identity	Description

BLAST of CSPI03G21370 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A5A7V5N8	9.3e-41	56.16	GPI-anchor transamidase isoform X1 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C...	[more]
A0A5D3DE60	8.2e-37	50.65	zf-RVT domain-containing protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676...	[more]
A0A5A7T2Y0	2.6e-35	53.52	zf-RVT domain-containing protein OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27...	[more]
A0A6J1DIE2	1.1e-25	43.71	uncharacterized protein LOC111020765 OS=Momordica charantia OX=3673 GN=LOC111020...	[more]
A0A5D3DC06	3.1e-20	40.85	Mediator of RNA polymerase II transcription subunit 8 isoform X2 OS=Cucumis melo...	[more]

BLAST of CSPI03G21370 vs. NCBI nr


Match Name	E-value	Identity	Description
KAA0062564.1	1.9e-40	56.16	GPI-anchor transamidase isoform X1 [Cucumis melo var. makuwa]	[more]
TYK21876.1	1.7e-36	50.65	hypothetical protein E5676_scaffold494G00090 [Cucumis melo var. makuwa]	[more]
KAA0035739.1	5.4e-35	53.52	hypothetical protein E6C27_scaffold403G00100 [Cucumis melo var. makuwa]	[more]
XP_038903695.1	9.2e-35	47.74	uncharacterized protein LOC120090219 [Benincasa hispida]	[more]
XP_022153214.1	2.3e-25	43.71	uncharacterized protein LOC111020765 [Momordica charantia]	[more]

BLAST of CSPI03G21370 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G25270.1	1.3e-05	27.78	Ribonuclease H-like superfamily protein	[more]

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CSPI03G21370.1	CSPI03G21370.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006506	GPI anchor biosynthetic process
cellular_component	GO:0005737	cytoplasm
cellular_component	GO:0043231	intracellular membrane-bounded organelle
cellular_component	GO:0032991	protein-containing complex
molecular_function	GO:0004197	cysteine-type endopeptidase activity
molecular_function	GO:0016788	hydrolase activity, acting on ester bonds