CSPI03G20910 (gene) Cucumber (PI 183967) v1

Overview


Name	CSPI03G20910
Type	gene
Organism	Cucumis sativus var. hardwickii cv. PI 183967 (Cucumber (PI 183967) v1)
Description	Valyl-tRNA synthetase
Location	Chr3: 16966213 .. 16967079 (-)
RNA-Seq Expression	CSPI03G20910
Synteny	CSPI03G20910

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGAAATGCCAGCTGGTGTGTCGGACACGTGTCAGAGGTGTCGGGGGTGTCAGTGTTGGACACGCTTGGCCATTTTAGGAGTGTTTGTGCTTCTTAGGTCTTAGTCACATTATTTAGGTTCTAGCTAAAGAGCTCTGTTATTGTTATCTTGTTTTTCCTTTCTCTAAGCCTGTAGAAGGGATTATGCATTGGGGCTAACATTTCAATTATGTTTTATCAGTAGTGATTACTTGACAGTAGCAACAACTCGTCCTGAAACCTTATTTGGTGATGTAGCTATAGCAGTGCATCCTCAGGTAAGCTGGCATTCAACTATTTACAGTTTAACTTGGGTTGGTGATGGTGGATGGATACTTATAACTAGTTCCATCTCTCTGGGTTTTTAAGGACACTATGGATGATGAAATTGATAGTCAAGGTGCACACAAGATGACTTATACTTAAAACAATGAAAAGAAAAAGGTCTTAGAGAGATATAACTAGTTCTAGCTCTTTAAGTATGCAATGAAAATGATCTAAAGGTCTGAATATTTTTTGTTTGGTGAGTCAATATCCTGATAGGAAACATGGACATATTTGAAGCTTAGCATAGGACTTAAAAAGAATAGTAGTGTCTTGCTTAAATATCATGGATATGTCCACCCTCACTGATGTTTCTTGCTAATTAATAGGAAACTGATTCTTGATCCTTATAGTGGGATGACTTTATTTGCAGGATGATCGTTATTCTAAATATGTTGGCATGATGGCAATTGTTCCAATGACGTATGGTCGTCATGTCCCTATCATCTCTGATAAGGTAACCATATTCTCAATTACCTTATCTATTTGAGGTAATTATCATGATTCAACTTGGTAGAGATGAA

mRNA sequence

ATGGAAATGCCAGCTGGTGTGTCGGACACGTGTCAGAGGTGTCGGGGGTGTCAGTGTTGGACACGCTTGGCCATTTTAGGAGTGTTTGTGCTTCTTAGTAGTGATTACTTGACAGTAGCAACAACTCGTCCTGAAACCTTATTTGGTGATGTAGCTATAGCAGTGCATCCTCAGGATGATCGTTATTCTAAATATGTTGGCATGATGGCAATTGTTCCAATGACGTATGGTCGTCATGTCCCTATCATCTCTGATAAGGTAACCATATTCTCAATTACCTTATCTATTTGAGGTAATTATCATGATTCAACTTGGTAGAGATGAA

Coding sequence (CDS)

ATGGAAATGCCAGCTGGTGTGTCGGACACGTGTCAGAGGTGTCGGGGGTGTCAGTGTTGGACACGCTTGGCCATTTTAGGAGTGTTTGTGCTTCTTAGTAGTGATTACTTGACAGTAGCAACAACTCGTCCTGAAACCTTATTTGGTGATGTAGCTATAGCAGTGCATCCTCAGGATGATCGTTATTCTAAATATGTTGGCATGATGGCAATTGTTCCAATGACGTATGGTCGTCATGTCCCTATCATCTCTGATAAGGTAACCATATTCTCAATTACCTTATCTATTTGA

Protein sequence

MEMPAGVSDTCQRCRGCQCWTRLAILGVFVLLSSDYLTVATTRPETLFGDVAIAVHPQDDRYSKYVGMMAIVPMTYGRHVPIISDKVTIFSITLSI*

Homology

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BLAST of CSPI03G20910 vs. ExPASy Swiss-Prot
Match: F4KE63 (Valine--tRNA ligase, chloroplastic/mitochondrial 2 OS=Arabidopsis thaliana OX=3702 GN=EMB2247 PE=3 SV=1)

HSP 1 Score: 103.2 bits (256), Expect = 1.7e-21
Identity = 46/54 (85.19%), Postives = 51/54 (94.44%), Query Frame = 0


Query: 33  SSDYLTVATTRPETLFGDVAIAVHPQDDRYSKYVGMMAIVPMTYGRHVPIISDK 87
           S D+LT+ATTRPETLFGDVA+AVHP+DDRYSKYVG  AIVPMTYGRHVPII+DK
Sbjct: 273 SPDFLTIATTRPETLFGDVALAVHPEDDRYSKYVGQTAIVPMTYGRHVPIIADK 326

[Close]

BLAST of CSPI03G20910 vs. ExPASy Swiss-Prot
Match: Q9HXH0 (Valine--tRNA ligase OS=Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1) OX=208964 GN=valS PE=1 SV=1)

HSP 1 Score: 75.9 bits (185), Expect = 2.9e-13
Identity = 33/52 (63.46%), Postives = 43/52 (82.69%), Query Frame = 0


Query: 35  DYLTVATTRPETLFGDVAIAVHPQDDRYSKYVGMMAIVPMTYGRHVPIISDK 87
           DYL VATTRPETL GD A+AVHP+D+RY+K +G  A +P+  GRH+PII+D+
Sbjct: 212 DYLVVATTRPETLLGDAAVAVHPEDERYAKLIGQFAELPIV-GRHIPIIADE 262

[Close]

BLAST of CSPI03G20910 vs. ExPASy Swiss-Prot
Match: A7HJX5 (Valine--tRNA ligase OS=Fervidobacterium nodosum (strain ATCC 35602 / DSM 5306 / Rt17-B1) OX=381764 GN=valS PE=3 SV=1)

HSP 1 Score: 74.3 bits (181), Expect = 8.3e-13
Identity = 31/51 (60.78%), Postives = 42/51 (82.35%), Query Frame = 0


Query: 35  DYLTVATTRPETLFGDVAIAVHPQDDRYSKYVGMMAIVPMTYGRHVPIISD 86
           DY+ +ATTRPET+ GD A+AVHP D+RY KYVG +AI+P+  GR +P+I+D
Sbjct: 208 DYIIIATTRPETMLGDTAVAVHPSDERYRKYVGKIAILPLV-GREIPVIAD 257

[Close]

BLAST of CSPI03G20910 vs. ExPASy Swiss-Prot
Match: Q89AG3 (Valine--tRNA ligase OS=Buchnera aphidicola subsp. Baizongia pistaciae (strain Bp) OX=224915 GN=valS PE=3 SV=1)

HSP 1 Score: 73.2 bits (178), Expect = 1.9e-12
Identity = 34/51 (66.67%), Postives = 40/51 (78.43%), Query Frame = 0


Query: 36  YLTVATTRPETLFGDVAIAVHPQDDRYSKYVGMMAIVPMTYGRHVPIISDK 87
           YL +ATTRPETLFGD AIAVHP D RY KY+G  A+VP+   R +PIISD+
Sbjct: 218 YLVIATTRPETLFGDTAIAVHPNDSRYKKYIGCYALVPI-INRIIPIISDE 267

[Close]

BLAST of CSPI03G20910 vs. ExPASy Swiss-Prot
Match: Q7VQT2 (Valine--tRNA ligase OS=Blochmannia floridanus OX=203907 GN=valS PE=3 SV=1)

HSP 1 Score: 72.4 bits (176), Expect = 3.2e-12
Identity = 33/54 (61.11%), Postives = 42/54 (77.78%), Query Frame = 0


Query: 33  SSDYLTVATTRPETLFGDVAIAVHPQDDRYSKYVGMMAIVPMTYGRHVPIISDK 87
           ++DYLT+ATTRPET+ GDVAIAV+P+D RYS  +G     P+T  R +PIISDK
Sbjct: 223 NADYLTIATTRPETILGDVAIAVNPEDPRYSHLIGKYVFTPIT-NRRIPIISDK 275

[Close]

BLAST of CSPI03G20910 vs. ExPASy TrEMBL
Match: A0A6J1IZ88 (Valyl-tRNA synthetase OS=Cucurbita maxima OX=3661 GN=LOC111482028 PE=3 SV=1)

HSP 1 Score: 112.5 bits (280), Expect = 1.0e-21
Identity = 53/53 (100.00%), Postives = 53/53 (100.00%), Query Frame = 0


Query: 34  SDYLTVATTRPETLFGDVAIAVHPQDDRYSKYVGMMAIVPMTYGRHVPIISDK 87
           SDYLTVATTRPETLFGDVAIAVHPQDDRYSKYVGMMAIVPMTYGRHVPIISDK
Sbjct: 270 SDYLTVATTRPETLFGDVAIAVHPQDDRYSKYVGMMAIVPMTYGRHVPIISDK 322

[Close]

BLAST of CSPI03G20910 vs. ExPASy TrEMBL
Match: A0A6J1J7Q1 (Valyl-tRNA synthetase OS=Cucurbita maxima OX=3661 GN=LOC111482028 PE=4 SV=1)

HSP 1 Score: 112.5 bits (280), Expect = 1.0e-21
Identity = 53/53 (100.00%), Postives = 53/53 (100.00%), Query Frame = 0


Query: 34  SDYLTVATTRPETLFGDVAIAVHPQDDRYSKYVGMMAIVPMTYGRHVPIISDK 87
           SDYLTVATTRPETLFGDVAIAVHPQDDRYSKYVGMMAIVPMTYGRHVPIISDK
Sbjct: 124 SDYLTVATTRPETLFGDVAIAVHPQDDRYSKYVGMMAIVPMTYGRHVPIISDK 176

[Close]

BLAST of CSPI03G20910 vs. ExPASy TrEMBL
Match: A0A6J1F580 (Valyl-tRNA synthetase OS=Cucurbita moschata OX=3662 GN=LOC111442245 PE=3 SV=1)

HSP 1 Score: 112.5 bits (280), Expect = 1.0e-21
Identity = 53/53 (100.00%), Postives = 53/53 (100.00%), Query Frame = 0


Query: 34  SDYLTVATTRPETLFGDVAIAVHPQDDRYSKYVGMMAIVPMTYGRHVPIISDK 87
           SDYLTVATTRPETLFGDVAIAVHPQDDRYSKYVGMMAIVPMTYGRHVPIISDK
Sbjct: 270 SDYLTVATTRPETLFGDVAIAVHPQDDRYSKYVGMMAIVPMTYGRHVPIISDK 322

[Close]

BLAST of CSPI03G20910 vs. ExPASy TrEMBL
Match: A0A6J1FA83 (Valyl-tRNA synthetase OS=Cucurbita moschata OX=3662 GN=LOC111442245 PE=4 SV=1)

HSP 1 Score: 112.5 bits (280), Expect = 1.0e-21
Identity = 53/53 (100.00%), Postives = 53/53 (100.00%), Query Frame = 0


Query: 34  SDYLTVATTRPETLFGDVAIAVHPQDDRYSKYVGMMAIVPMTYGRHVPIISDK 87
           SDYLTVATTRPETLFGDVAIAVHPQDDRYSKYVGMMAIVPMTYGRHVPIISDK
Sbjct: 124 SDYLTVATTRPETLFGDVAIAVHPQDDRYSKYVGMMAIVPMTYGRHVPIISDK 176

[Close]

BLAST of CSPI03G20910 vs. ExPASy TrEMBL
Match: A0A5N6RLU1 (Valyl-tRNA synthetase OS=Carpinus fangiana OX=176857 GN=FH972_017719 PE=3 SV=1)

HSP 1 Score: 110.9 bits (276), Expect = 3.0e-21
Identity = 50/54 (92.59%), Postives = 54/54 (100.00%), Query Frame = 0


Query: 33  SSDYLTVATTRPETLFGDVAIAVHPQDDRYSKYVGMMAIVPMTYGRHVPIISDK 87
           SSDYLT+ATTRPETLFGDVAIAVHP+DDRYSKY+GMMAIVPMTYGRHVPIISD+
Sbjct: 269 SSDYLTIATTRPETLFGDVAIAVHPKDDRYSKYIGMMAIVPMTYGRHVPIISDR 322

[Close]

BLAST of CSPI03G20910 vs. NCBI nr
Match: XP_011651387.1 (valine--tRNA ligase, chloroplastic/mitochondrial 2 isoform X1 [Cucumis sativus] >KAE8650589.1 hypothetical protein Csa_011094 [Cucumis sativus])

HSP 1 Score: 114.4 bits (285), Expect = 5.5e-22
Identity = 54/54 (100.00%), Postives = 54/54 (100.00%), Query Frame = 0


Query: 33  SSDYLTVATTRPETLFGDVAIAVHPQDDRYSKYVGMMAIVPMTYGRHVPIISDK 87
           SSDYLTVATTRPETLFGDVAIAVHPQDDRYSKYVGMMAIVPMTYGRHVPIISDK
Sbjct: 269 SSDYLTVATTRPETLFGDVAIAVHPQDDRYSKYVGMMAIVPMTYGRHVPIISDK 322

[Close]

BLAST of CSPI03G20910 vs. NCBI nr
Match: XP_011651390.1 (valine--tRNA ligase, chloroplastic/mitochondrial 2 isoform X2 [Cucumis sativus])

HSP 1 Score: 114.4 bits (285), Expect = 5.5e-22
Identity = 54/54 (100.00%), Postives = 54/54 (100.00%), Query Frame = 0


Query: 33  SSDYLTVATTRPETLFGDVAIAVHPQDDRYSKYVGMMAIVPMTYGRHVPIISDK 87
           SSDYLTVATTRPETLFGDVAIAVHPQDDRYSKYVGMMAIVPMTYGRHVPIISDK
Sbjct: 123 SSDYLTVATTRPETLFGDVAIAVHPQDDRYSKYVGMMAIVPMTYGRHVPIISDK 176

[Close]

BLAST of CSPI03G20910 vs. NCBI nr
Match: XP_022983417.1 (valine--tRNA ligase, chloroplastic/mitochondrial 2 isoform X1 [Cucurbita maxima])

HSP 1 Score: 112.5 bits (280), Expect = 2.1e-21
Identity = 53/53 (100.00%), Postives = 53/53 (100.00%), Query Frame = 0


Query: 34  SDYLTVATTRPETLFGDVAIAVHPQDDRYSKYVGMMAIVPMTYGRHVPIISDK 87
           SDYLTVATTRPETLFGDVAIAVHPQDDRYSKYVGMMAIVPMTYGRHVPIISDK
Sbjct: 270 SDYLTVATTRPETLFGDVAIAVHPQDDRYSKYVGMMAIVPMTYGRHVPIISDK 322

[Close]

BLAST of CSPI03G20910 vs. NCBI nr
Match: KAG6581186.1 (Valine--tRNA ligase, chloroplastic/mitochondrial 2, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 112.5 bits (280), Expect = 2.1e-21
Identity = 53/53 (100.00%), Postives = 53/53 (100.00%), Query Frame = 0


Query: 34  SDYLTVATTRPETLFGDVAIAVHPQDDRYSKYVGMMAIVPMTYGRHVPIISDK 87
           SDYLTVATTRPETLFGDVAIAVHPQDDRYSKYVGMMAIVPMTYGRHVPIISDK
Sbjct: 270 SDYLTVATTRPETLFGDVAIAVHPQDDRYSKYVGMMAIVPMTYGRHVPIISDK 322

[Close]

BLAST of CSPI03G20910 vs. NCBI nr
Match: XP_022935323.1 (valine--tRNA ligase, chloroplastic/mitochondrial 2 isoform X2 [Cucurbita moschata])

HSP 1 Score: 112.5 bits (280), Expect = 2.1e-21
Identity = 53/53 (100.00%), Postives = 53/53 (100.00%), Query Frame = 0


Query: 34  SDYLTVATTRPETLFGDVAIAVHPQDDRYSKYVGMMAIVPMTYGRHVPIISDK 87
           SDYLTVATTRPETLFGDVAIAVHPQDDRYSKYVGMMAIVPMTYGRHVPIISDK
Sbjct: 124 SDYLTVATTRPETLFGDVAIAVHPQDDRYSKYVGMMAIVPMTYGRHVPIISDK 176

[Close]

BLAST of CSPI03G20910 vs. TAIR 10
Match: AT5G16715.1 (ATP binding;valine-tRNA ligases;aminoacyl-tRNA ligases;nucleotide binding;ATP binding;aminoacyl-tRNA ligases )

HSP 1 Score: 103.2 bits (256), Expect = 1.2e-22
Identity = 46/54 (85.19%), Postives = 51/54 (94.44%), Query Frame = 0


Query: 33  SSDYLTVATTRPETLFGDVAIAVHPQDDRYSKYVGMMAIVPMTYGRHVPIISDK 87
           S D+LT+ATTRPETLFGDVA+AVHP+DDRYSKYVG  AIVPMTYGRHVPII+DK
Sbjct: 273 SPDFLTIATTRPETLFGDVALAVHPEDDRYSKYVGQTAIVPMTYGRHVPIIADK 326

[Close]

BLAST of CSPI03G20910 vs. TAIR 10
Match: AT1G14610.1 (valyl-tRNA synthetase / valine--tRNA ligase (VALRS) )

HSP 1 Score: 52.8 bits (125), Expect = 1.8e-07
Identity = 26/51 (50.98%), Postives = 32/51 (62.75%), Query Frame = 0


Query: 39  VATTRPETLFGDVAIAVHPQDDRYSKYVGMMAIVPMTYGRHVPIISDKVTI 90
           VATTR ET+ GD AIA+HP D RY    G  A+ P   GR +PII D + +
Sbjct: 364 VATTRVETMLGDTAIAIHPDDARYKHLHGKFAVHPFN-GRKLPIICDGILV 413

The following BLAST results are available for this feature:

BLAST of CSPI03G20910 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
F4KE63	1.7e-21	85.19	Valine--tRNA ligase, chloroplastic/mitochondrial 2 OS=Arabidopsis thaliana OX=37...	[more]
Q9HXH0	2.9e-13	63.46	Valine--tRNA ligase OS=Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / C...	[more]
A7HJX5	8.3e-13	60.78	Valine--tRNA ligase OS=Fervidobacterium nodosum (strain ATCC 35602 / DSM 5306 / ...	[more]
Q89AG3	1.9e-12	66.67	Valine--tRNA ligase OS=Buchnera aphidicola subsp. Baizongia pistaciae (strain Bp...	[more]
Q7VQT2	3.2e-12	61.11	Valine--tRNA ligase OS=Blochmannia floridanus OX=203907 GN=valS PE=3 SV=1	[more]

BLAST of CSPI03G20910 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1IZ88	1.0e-21	100.00	Valyl-tRNA synthetase OS=Cucurbita maxima OX=3661 GN=LOC111482028 PE=3 SV=1	[more]
A0A6J1J7Q1	1.0e-21	100.00	Valyl-tRNA synthetase OS=Cucurbita maxima OX=3661 GN=LOC111482028 PE=4 SV=1	[more]
A0A6J1F580	1.0e-21	100.00	Valyl-tRNA synthetase OS=Cucurbita moschata OX=3662 GN=LOC111442245 PE=3 SV=1	[more]
A0A6J1FA83	1.0e-21	100.00	Valyl-tRNA synthetase OS=Cucurbita moschata OX=3662 GN=LOC111442245 PE=4 SV=1	[more]
A0A5N6RLU1	3.0e-21	92.59	Valyl-tRNA synthetase OS=Carpinus fangiana OX=176857 GN=FH972_017719 PE=3 SV=1	[more]

BLAST of CSPI03G20910 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_011651387.1	5.5e-22	100.00	valine--tRNA ligase, chloroplastic/mitochondrial 2 isoform X1 [Cucumis sativus] ...	[more]
XP_011651390.1	5.5e-22	100.00	valine--tRNA ligase, chloroplastic/mitochondrial 2 isoform X2 [Cucumis sativus]	[more]
XP_022983417.1	2.1e-21	100.00	valine--tRNA ligase, chloroplastic/mitochondrial 2 isoform X1 [Cucurbita maxima]	[more]
KAG6581186.1	2.1e-21	100.00	Valine--tRNA ligase, chloroplastic/mitochondrial 2, partial [Cucurbita argyrospe...	[more]
XP_022935323.1	2.1e-21	100.00	valine--tRNA ligase, chloroplastic/mitochondrial 2 isoform X2 [Cucurbita moschat...	[more]

BLAST of CSPI03G20910 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G16715.1	1.2e-22	85.19	ATP binding;valine-tRNA ligases;aminoacyl-tRNA ligases;nucleotide binding;ATP bi...	[more]
AT1G14610.1	1.8e-07	50.98	valyl-tRNA synthetase / valine--tRNA ligase (VALRS)	[more]

InterPro

Analysis Name: InterPro Annotations of Cucumber (PI 183967) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR002300	Aminoacyl-tRNA synthetase, class Ia	PFAM	PF00133	tRNA-synt_1	coord: 36..60 e-value: 2.2E-6 score: 26.3
IPR009008	Valyl/Leucyl/Isoleucyl-tRNA synthetase, editing domain	GENE3D	3.90.740.10		coord: 26..89 e-value: 6.8E-15 score: 56.9
IPR009008	Valyl/Leucyl/Isoleucyl-tRNA synthetase, editing domain	SUPERFAMILY	50677	ValRS/IleRS/LeuRS editing domain	coord: 29..87
IPR002303	Valine-tRNA ligase	PANTHER	PTHR11946	VALYL-TRNA SYNTHETASES	coord: 33..86
None	No IPR available	PANTHER	PTHR11946:SF93	VALYL-TRNA SYNTHETASE, ISOFORM C	coord: 33..86

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CSPI03G20910.1	CSPI03G20910.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0106074	aminoacyl-tRNA metabolism involved in translational fidelity
biological_process	GO:0006438	valyl-tRNA aminoacylation
biological_process	GO:0006418	tRNA aminoacylation for protein translation
cellular_component	GO:0005737	cytoplasm
molecular_function	GO:0002161	aminoacyl-tRNA editing activity
molecular_function	GO:0005524	ATP binding
molecular_function	GO:0004832	valine-tRNA ligase activity
molecular_function	GO:0004812	aminoacyl-tRNA ligase activity
molecular_function	GO:0000166	nucleotide binding