CSPI03G20310 (gene) Cucumber (PI 183967) v1

Overview
NameCSPI03G20310
Typegene
OrganismCucumis sativus var. hardwickii cv. PI 183967 (Cucumber (PI 183967) v1)
DescriptionMADS-box domain-containing protein
LocationChr3: 16120154 .. 16120804 (-)
RNA-Seq ExpressionCSPI03G20310
SyntenyCSPI03G20310
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGGGAGAGGAATATTAAGTCTTAAGCTCATTCCCAATCCCAAAAGTCGAAGAACAACCTTTCTTAAGAGGAAGAAAAGTCTGATTAAGAAGGCTTACGAGCTTTCCACCCTTTGCGATGTTCAAACTTGCCTTTTCATTGCTTCTGACTGTGACCCCTCCACTCACTTCGAAACTTGGCCTCCCAATCATCACCAAATCCACCAGATGATCCGTTCTTATAAATCCCACTCTTTCACCAAACCCAACTCCTCTTATGATCTTAACCGCTTCTTCTCTGATCGCAAGAACAAAATCCTAACTAACACATCTAAACTTCTCCACAATGTTGTGGATCACCAATCCGAACATCAATTGATGGAGTTGTTGGATGCTTTGGATTCCAAGATTAGAGTTGCAAACGACATGATTGAGTTCATGGAGGCCGATTATGATCACTTGATTGACCAAGCAATTGGCATGGATACACCTCCTTCCCAAACAGAGGATGAGGAAACCACTCAATTTAACATTTCAGACCTTTTTAATGAGCCAGATGAGTTTGAAGAATACAACGTCGAGGGTTTCCAATCTCTGCTTGAGGACAAGTTTCTGGAAACTCTGGTTCAGAACAATCCCATTCCAGACTTTGATTTTGATTTTGATTACTAA

mRNA sequence

ATGGGGAGAGGAATATTAAGTCTTAAGCTCATTCCCAATCCCAAAAGTCGAAGAACAACCTTTCTTAAGAGGAAGAAAAGTCTGATTAAGAAGGCTTACGAGCTTTCCACCCTTTGCGATGTTCAAACTTGCCTTTTCATTGCTTCTGACTGTGACCCCTCCACTCACTTCGAAACTTGGCCTCCCAATCATCACCAAATCCACCAGATGATCCGTTCTTATAAATCCCACTCTTTCACCAAACCCAACTCCTCTTATGATCTTAACCGCTTCTTCTCTGATCGCAAGAACAAAATCCTAACTAACACATCTAAACTTCTCCACAATGTTGTGGATCACCAATCCGAACATCAATTGATGGAGTTGTTGGATGCTTTGGATTCCAAGATTAGAGTTGCAAACGACATGATTGAGTTCATGGAGGCCGATTATGATCACTTGATTGACCAAGCAATTGGCATGGATACACCTCCTTCCCAAACAGAGGATGAGGAAACCACTCAATTTAACATTTCAGACCTTTTTAATGAGCCAGATGAGTTTGAAGAATACAACGTCGAGGGTTTCCAATCTCTGCTTGAGGACAAGTTTCTGGAAACTCTGGTTCAGAACAATCCCATTCCAGACTTTGATTTTGATTTTGATTACTAA

Coding sequence (CDS)

ATGGGGAGAGGAATATTAAGTCTTAAGCTCATTCCCAATCCCAAAAGTCGAAGAACAACCTTTCTTAAGAGGAAGAAAAGTCTGATTAAGAAGGCTTACGAGCTTTCCACCCTTTGCGATGTTCAAACTTGCCTTTTCATTGCTTCTGACTGTGACCCCTCCACTCACTTCGAAACTTGGCCTCCCAATCATCACCAAATCCACCAGATGATCCGTTCTTATAAATCCCACTCTTTCACCAAACCCAACTCCTCTTATGATCTTAACCGCTTCTTCTCTGATCGCAAGAACAAAATCCTAACTAACACATCTAAACTTCTCCACAATGTTGTGGATCACCAATCCGAACATCAATTGATGGAGTTGTTGGATGCTTTGGATTCCAAGATTAGAGTTGCAAACGACATGATTGAGTTCATGGAGGCCGATTATGATCACTTGATTGACCAAGCAATTGGCATGGATACACCTCCTTCCCAAACAGAGGATGAGGAAACCACTCAATTTAACATTTCAGACCTTTTTAATGAGCCAGATGAGTTTGAAGAATACAACGTCGAGGGTTTCCAATCTCTGCTTGAGGACAAGTTTCTGGAAACTCTGGTTCAGAACAATCCCATTCCAGACTTTGATTTTGATTTTGATTACTAA

Protein sequence

MGRGILSLKLIPNPKSRRTTFLKRKKSLIKKAYELSTLCDVQTCLFIASDCDPSTHFETWPPNHHQIHQMIRSYKSHSFTKPNSSYDLNRFFSDRKNKILTNTSKLLHNVVDHQSEHQLMELLDALDSKIRVANDMIEFMEADYDHLIDQAIGMDTPPSQTEDEETTQFNISDLFNEPDEFEEYNVEGFQSLLEDKFLETLVQNNPIPDFDFDFDY*
Homology
BLAST of CSPI03G20310 vs. ExPASy Swiss-Prot
Match: Q9FIM0 (Agamous-like MADS-box protein AGL82 OS=Arabidopsis thaliana OX=3702 GN=AGL82 PE=1 SV=1)

HSP 1 Score: 62.4 bits (150), Expect = 7.3e-09
Identity = 60/194 (30.93%), Postives = 93/194 (47.94%), Query Frame = 0

Query: 5   ILSLKLIPNPKSRRTTFLKRKKSLIKKAYELSTLCDVQTCLFIASDCDPS----THFETW 64
           ++ L+ I N K+R TT+ KRK SL KKA E STLC V+TCL +      +    +  E W
Sbjct: 5   VVDLQRIANDKTRITTYKKRKASLYKKAQEFSTLCGVETCLIVYGPTKATDVVISEPEIW 64

Query: 65  PPNHHQIHQMIRSYKSHSFTKPNSSYDLNRFFSD--RKNKILT-------NTSKLLHNVV 124
           P +  ++  +IR YK    T      ++  F +D  + N+++T       N        +
Sbjct: 65  PKDETKVRAIIRKYKDTVSTSCRKETNVETFVNDVGKGNEVVTKKRVKRENKYSSWEEKL 124

Query: 125 DHQSEHQLMELLDALDSKIRVANDMIEFMEADYDHLIDQAIGMDTP-PSQTEDEETTQFN 184
           D  S  QL  +  A+DSK+   N+ +   E     +  QA  MDTP P    D+   QF 
Sbjct: 125 DKCSREQLHGIFCAVDSKL---NEAVTRQERSMFRVNHQA--MDTPFPQNLMDQ---QF- 184

BLAST of CSPI03G20310 vs. ExPASy Swiss-Prot
Match: P29383 (Agamous-like MADS-box protein AGL3 OS=Arabidopsis thaliana OX=3702 GN=AGL3 PE=1 SV=2)

HSP 1 Score: 58.9 bits (141), Expect = 8.1e-08
Identity = 44/129 (34.11%), Postives = 64/129 (49.61%), Query Frame = 0

Query: 1   MGRGILSLKLIPNPKSRRTTFLKRKKSLIKKAYELSTLCDVQTCLFIASDCDPSTHFETW 60
           MGRG + LK I N  +R+ TF KR+  L+KKAYELS LCD +  L I S+      F + 
Sbjct: 1   MGRGKVELKRIENKINRQVTFAKRRNGLLKKAYELSVLCDAEIALLIFSNRGKLYEFCSS 60

Query: 61  PPNHHQIHQMIRSYKSHSFT--KPNSSYDLNRFFSDRKNKILTNTSKLLHNVVDHQSEHQ 120
           P     + + +  Y+ HS+    PN S    +   D+    L   S++   ++ H   H 
Sbjct: 61  PSG---MARTVDKYRKHSYATMDPNQS---AKDLQDKYQDYLKLKSRV--EILQHSQRHL 120

Query: 121 LMELLDALD 128
           L E L  +D
Sbjct: 121 LGEELSEMD 121

BLAST of CSPI03G20310 vs. ExPASy Swiss-Prot
Match: Q38694 (Agamous-like MADS-box protein AGL9 homolog OS=Aranda deborah OX=29714 PE=2 SV=1)

HSP 1 Score: 57.8 bits (138), Expect = 1.8e-07
Identity = 28/50 (56.00%), Postives = 36/50 (72.00%), Query Frame = 0

Query: 1  MGRGILSLKLIPNPKSRRTTFLKRKKSLIKKAYELSTLCDVQTCLFIASD 51
          MGRG + LK+I N  +R+ TF KR+K L+KKAYELS LCD +  L I S+
Sbjct: 1  MGRGRVELKMIENKINRQVTFAKRRKRLLKKAYELSVLCDAEVALIIFSN 50

BLAST of CSPI03G20310 vs. ExPASy Swiss-Prot
Match: F6I457 (Agamous-like MADS-box protein AGL11 OS=Vitis vinifera OX=29760 GN=AGL11 PE=2 SV=1)

HSP 1 Score: 55.8 bits (133), Expect = 6.9e-07
Identity = 46/166 (27.71%), Postives = 81/166 (48.80%), Query Frame = 0

Query: 1   MGRGILSLKLIPNPKSRRTTFLKRKKSLIKKAYELSTLCDVQTCLFIASDCDPSTHFETW 60
           MGRG + +K I N  +R+ TF KR+  L+KKAYELS LCD +  L +      S+    +
Sbjct: 1   MGRGKIEIKRIENTTNRQVTFCKRRNGLLKKAYELSVLCDAEVALIVF-----SSRGRVY 60

Query: 61  PPNHHQIHQMIRSYK--SHSFTKPNSSYDLNRFFSDRKNKILTNTSKLLHNVVDHQSEHQ 120
             +++ I   I  YK  S   T   S+ ++N  +  +++  L    ++L N   H     
Sbjct: 61  EYSNNNIKSTIDRYKKASSDSTNGGSTMEINAQYYQQESAKLRQQIQMLQNSNRHLMGDS 120

Query: 121 LMEL----LDALDSKI-----RVANDMIEFMEADYDHLIDQAIGMD 156
           L  L    L  L++++     R+ +   E + A+ ++L  + I ++
Sbjct: 121 LASLTVKELKQLENRLERGITRIRSKKHELLLAEIEYLQKREIELE 161

BLAST of CSPI03G20310 vs. ExPASy Swiss-Prot
Match: A0A217EJJ0 (Agamous-like MADS-box protein AGL11 OS=Vitis vinifera OX=29760 GN=AGL11 PE=3 SV=1)

HSP 1 Score: 55.8 bits (133), Expect = 6.9e-07
Identity = 46/166 (27.71%), Postives = 81/166 (48.80%), Query Frame = 0

Query: 1   MGRGILSLKLIPNPKSRRTTFLKRKKSLIKKAYELSTLCDVQTCLFIASDCDPSTHFETW 60
           MGRG + +K I N  +R+ TF KR+  L+KKAYELS LCD +  L +      S+    +
Sbjct: 1   MGRGKIEIKRIENTTNRQVTFCKRRNGLLKKAYELSVLCDAEVALIVF-----SSRGRVY 60

Query: 61  PPNHHQIHQMIRSYK--SHSFTKPNSSYDLNRFFSDRKNKILTNTSKLLHNVVDHQSEHQ 120
             +++ I   I  YK  S   T   S+ ++N  +  +++  L    ++L N   H     
Sbjct: 61  EYSNNNIKSTIDRYKKASSDSTNGGSTMEINAQYYQQESAKLRQQIQMLQNSNRHLMGDS 120

Query: 121 LMEL----LDALDSKI-----RVANDMIEFMEADYDHLIDQAIGMD 156
           L  L    L  L++++     R+ +   E + A+ ++L  + I ++
Sbjct: 121 LASLTVKELKQLENRLERGITRIRSKKHELLLAEIEYLQKREIELE 161

BLAST of CSPI03G20310 vs. ExPASy TrEMBL
Match: A0A0A0LAL9 (MADS-box domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G258140 PE=4 SV=1)

HSP 1 Score: 435.3 bits (1118), Expect = 1.5e-118
Identity = 215/216 (99.54%), Postives = 216/216 (100.00%), Query Frame = 0

Query: 1   MGRGILSLKLIPNPKSRRTTFLKRKKSLIKKAYELSTLCDVQTCLFIASDCDPSTHFETW 60
           MGRGILSLKLIPNPKSRRTTFLKRKKSLIKKAYELSTLCDVQTCLFIASDCDPSTHFETW
Sbjct: 1   MGRGILSLKLIPNPKSRRTTFLKRKKSLIKKAYELSTLCDVQTCLFIASDCDPSTHFETW 60

Query: 61  PPNHHQIHQMIRSYKSHSFTKPNSSYDLNRFFSDRKNKILTNTSKLLHNVVDHQSEHQLM 120
           PPNHHQIHQMIRSYKSHSFTKPNSSYDLNRFFSDRKNKILTNTSKLLHNVVDHQSEHQLM
Sbjct: 61  PPNHHQIHQMIRSYKSHSFTKPNSSYDLNRFFSDRKNKILTNTSKLLHNVVDHQSEHQLM 120

Query: 121 ELLDALDSKIRVANDMIEFMEADYDHLIDQAIGMDTPPSQTEDEETTQFNISDLFNEPDE 180
           ELLDALDSKIRVANDMIEFMEADYDHLIDQAIGMDTPPSQTEDEETTQFN+SDLFNEPDE
Sbjct: 121 ELLDALDSKIRVANDMIEFMEADYDHLIDQAIGMDTPPSQTEDEETTQFNVSDLFNEPDE 180

Query: 181 FEEYNVEGFQSLLEDKFLETLVQNNPIPDFDFDFDY 217
           FEEYNVEGFQSLLEDKFLETLVQNNPIPDFDFDFDY
Sbjct: 181 FEEYNVEGFQSLLEDKFLETLVQNNPIPDFDFDFDY 216

BLAST of CSPI03G20310 vs. ExPASy TrEMBL
Match: A0A6J1F370 (agamous-like MADS-box protein AGL6 OS=Cucurbita moschata OX=3662 GN=LOC111441717 PE=4 SV=1)

HSP 1 Score: 191.4 bits (485), Expect = 3.9e-45
Identity = 119/210 (56.67%), Postives = 142/210 (67.62%), Query Frame = 0

Query: 1   MGRGILSLKLIPNPKSRRTTFLKRKKSLIKKAYELSTLCDVQTCLFIASDCDPSTHFETW 60
           MGRG LSLKLI NPKSRRTTF KRKKSL+KKAYELSTLCDVQTC+ IAS+      FETW
Sbjct: 1   MGRGTLSLKLISNPKSRRTTFQKRKKSLMKKAYELSTLCDVQTCVVIASE-----EFETW 60

Query: 61  PPNHHQIHQMIRSYKSHSFTKPN--SSYDLNRFFSDRKNKILTNTSKLLHNV-------- 120
           P +  Q+  MIRSYKS SF K +  SSYDLN+FFSDRKNKI+T TSKL   V        
Sbjct: 61  PSDRTQVEAMIRSYKSQSFCKQSAKSSYDLNQFFSDRKNKIVTETSKLREKVQKSKDTNS 120

Query: 121 ---VDHQSEHQLMELLDALDSKIRVANDMIEFMEADYDHLIDQAIGMDTPPSQ----TED 180
              +D  SEHQL  L+  LDSKI VA+ MIEFMEADYD+LI+ A+ + +   Q     E 
Sbjct: 121 DHCLDSLSEHQLKALMATLDSKIGVADSMIEFMEADYDYLIEGAVEISSIDKQIFSPLET 180

Query: 181 EETTQFNISDLFNEPDEFEEYNVEGFQSLL 194
           E + + NIS+L  E +   +Y +EG   +L
Sbjct: 181 EASPELNISELGMEAN--YDYLIEGDTEIL 203

BLAST of CSPI03G20310 vs. ExPASy TrEMBL
Match: A0A5D3DXK1 (Agamous-like MADS-box protein AGL3 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold600G00710 PE=4 SV=1)

HSP 1 Score: 166.4 bits (420), Expect = 1.3e-37
Identity = 85/97 (87.63%), Postives = 91/97 (93.81%), Query Frame = 0

Query: 70  MIRSYKSHSFTKPNSSYDLNRFFSDRKNKILTNTSKLLHNVVDHQSEHQLMELLDALDSK 129
           MIRSYKSHSFTKPNSSYDLNRFFSDRKNKILT+TSKLLHNV DHQS++QL+ELL ALDSK
Sbjct: 1   MIRSYKSHSFTKPNSSYDLNRFFSDRKNKILTHTSKLLHNVRDHQSKYQLVELLAALDSK 60

Query: 130 IRVANDMIEFMEADYDHLIDQAIGMDTPPSQTEDEET 167
           I V N+MIEFMEADYD LIDQA+GMDT PSQTEDEET
Sbjct: 61  ISVGNNMIEFMEADYDDLIDQAVGMDTSPSQTEDEET 97

BLAST of CSPI03G20310 vs. ExPASy TrEMBL
Match: A0A5A7U2J6 (Agamous-like MADS-box protein AGL3 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold600G00930 PE=4 SV=1)

HSP 1 Score: 123.2 bits (308), Expect = 1.3e-24
Identity = 65/99 (65.66%), Postives = 73/99 (73.74%), Query Frame = 0

Query: 136 MIEFMEADYDHLIDQAIGMDTPPSQTEDEETTQ--------------------FNISDLF 195
           MIEFMEADYD LIDQA+GMDTPP QTEDEETTQ                    FNISD  
Sbjct: 1   MIEFMEADYDDLIDQAVGMDTPPFQTEDEETTQFKIFNFLNEPDEFEDEETIEFNISDFL 60

Query: 196 NEPDEFEEYNVEGFQSLLEDKFLETLVQNNPIPDFDFDF 215
           N+P+EFEEY+VEGFQSLL+D+FLETLVQNNPI + DFD+
Sbjct: 61  NDPNEFEEYDVEGFQSLLQDEFLETLVQNNPILNIDFDY 99

BLAST of CSPI03G20310 vs. ExPASy TrEMBL
Match: A0A6J1J5U6 (agamous-like MADS-box protein AGL14 OS=Cucurbita maxima OX=3661 GN=LOC111482037 PE=4 SV=1)

HSP 1 Score: 118.2 bits (295), Expect = 4.2e-23
Identity = 89/229 (38.86%), Postives = 122/229 (53.28%), Query Frame = 0

Query: 1   MGRGILSLKLIPNPKSRRTTFLKRKKSLIKKAYELSTLCDVQTCLFI-----ASDCDP-- 60
           M RG LS  LI N KSR +TF +RK SL+KKAYELSTLCDV+TC+ I     A D DP  
Sbjct: 1   MVRGKLSFNLIRNRKSRCSTFKQRKNSLMKKAYELSTLCDVRTCVLINGPGFAPDEDPSL 60

Query: 61  -STHFETWPPNHHQIHQMIRSYKSHSFT-KPNSSYDLNRFFSDRKNKILTNTSKLLHNV- 120
            S  F TWP N  ++  MI +YK+ +   +    +DL+ +FSDRK K++T   K    V 
Sbjct: 61  SSVEFHTWPSNRSEVEHMIHAYKNGNCNLQKRKCFDLSDYFSDRKRKLVTEMCKHREKVG 120

Query: 121 ----------VDHQSEHQLMELLDALDSKIRVANDMIEFMEAD------------YDHLI 180
                     +D  SE QL E ++ LDS+I  A  MI+FM+AD            Y++++
Sbjct: 121 KIMWPDWDERLDCLSEQQLREFMNGLDSRIEAAGRMIDFMDADNENLFGMFSCEEYENVV 180

Query: 181 DQAIGMDTPPSQTEDEETTQFNISDLFNEPDEFE-EYNVEGFQSLLEDK 197
                  TP     + E+T  +++     PDE E   N E  + LLED+
Sbjct: 181 QMGGDSSTPVLSLVELESTD-SLNSPNPNPDEDEIGCNFENLEYLLEDE 228

BLAST of CSPI03G20310 vs. NCBI nr
Match: XP_004145650.1 (agamous-like MADS-box protein AGL82 [Cucumis sativus])

HSP 1 Score: 435.3 bits (1118), Expect = 3.2e-118
Identity = 215/216 (99.54%), Postives = 216/216 (100.00%), Query Frame = 0

Query: 1   MGRGILSLKLIPNPKSRRTTFLKRKKSLIKKAYELSTLCDVQTCLFIASDCDPSTHFETW 60
           MGRGILSLKLIPNPKSRRTTFLKRKKSLIKKAYELSTLCDVQTCLFIASDCDPSTHFETW
Sbjct: 1   MGRGILSLKLIPNPKSRRTTFLKRKKSLIKKAYELSTLCDVQTCLFIASDCDPSTHFETW 60

Query: 61  PPNHHQIHQMIRSYKSHSFTKPNSSYDLNRFFSDRKNKILTNTSKLLHNVVDHQSEHQLM 120
           PPNHHQIHQMIRSYKSHSFTKPNSSYDLNRFFSDRKNKILTNTSKLLHNVVDHQSEHQLM
Sbjct: 61  PPNHHQIHQMIRSYKSHSFTKPNSSYDLNRFFSDRKNKILTNTSKLLHNVVDHQSEHQLM 120

Query: 121 ELLDALDSKIRVANDMIEFMEADYDHLIDQAIGMDTPPSQTEDEETTQFNISDLFNEPDE 180
           ELLDALDSKIRVANDMIEFMEADYDHLIDQAIGMDTPPSQTEDEETTQFN+SDLFNEPDE
Sbjct: 121 ELLDALDSKIRVANDMIEFMEADYDHLIDQAIGMDTPPSQTEDEETTQFNVSDLFNEPDE 180

Query: 181 FEEYNVEGFQSLLEDKFLETLVQNNPIPDFDFDFDY 217
           FEEYNVEGFQSLLEDKFLETLVQNNPIPDFDFDFDY
Sbjct: 181 FEEYNVEGFQSLLEDKFLETLVQNNPIPDFDFDFDY 216

BLAST of CSPI03G20310 vs. NCBI nr
Match: KAE8650565.1 (hypothetical protein Csa_011366 [Cucumis sativus])

HSP 1 Score: 435.3 bits (1118), Expect = 3.2e-118
Identity = 215/216 (99.54%), Postives = 216/216 (100.00%), Query Frame = 0

Query: 1   MGRGILSLKLIPNPKSRRTTFLKRKKSLIKKAYELSTLCDVQTCLFIASDCDPSTHFETW 60
           MGRGILSLKLIPNPKSRRTTFLKRKKSLIKKAYELSTLCDVQTCLFIASDCDPSTHFETW
Sbjct: 35  MGRGILSLKLIPNPKSRRTTFLKRKKSLIKKAYELSTLCDVQTCLFIASDCDPSTHFETW 94

Query: 61  PPNHHQIHQMIRSYKSHSFTKPNSSYDLNRFFSDRKNKILTNTSKLLHNVVDHQSEHQLM 120
           PPNHHQIHQMIRSYKSHSFTKPNSSYDLNRFFSDRKNKILTNTSKLLHNVVDHQSEHQLM
Sbjct: 95  PPNHHQIHQMIRSYKSHSFTKPNSSYDLNRFFSDRKNKILTNTSKLLHNVVDHQSEHQLM 154

Query: 121 ELLDALDSKIRVANDMIEFMEADYDHLIDQAIGMDTPPSQTEDEETTQFNISDLFNEPDE 180
           ELLDALDSKIRVANDMIEFMEADYDHLIDQAIGMDTPPSQTEDEETTQFN+SDLFNEPDE
Sbjct: 155 ELLDALDSKIRVANDMIEFMEADYDHLIDQAIGMDTPPSQTEDEETTQFNVSDLFNEPDE 214

Query: 181 FEEYNVEGFQSLLEDKFLETLVQNNPIPDFDFDFDY 217
           FEEYNVEGFQSLLEDKFLETLVQNNPIPDFDFDFDY
Sbjct: 215 FEEYNVEGFQSLLEDKFLETLVQNNPIPDFDFDFDY 250

BLAST of CSPI03G20310 vs. NCBI nr
Match: XP_038877825.1 (agamous-like MADS-box protein MADS1 [Benincasa hispida])

HSP 1 Score: 297.0 bits (759), Expect = 1.3e-76
Identity = 167/234 (71.37%), Postives = 183/234 (78.21%), Query Frame = 0

Query: 1   MGRGILSLKLIPNPKSRRTTFLKRKKSLIKKAYELSTLCDVQTCLFIASDCDPSTHFETW 60
           MGRG LSLKLIPN KSRRTTF+KRKKSL+KKAYELSTLCDVQTC+FIASDCDPSTHFETW
Sbjct: 1   MGRGTLSLKLIPNLKSRRTTFVKRKKSLMKKAYELSTLCDVQTCVFIASDCDPSTHFETW 60

Query: 61  PPNHHQIHQMIRSYKSHSFTK---PNSSYDLNRFFSDRKNKILTNTSKLLHNVVDHQ--- 120
           PPNH QI+ MIRSYKS SF K    NSSY+L +FFSDRKNKILT+TSKL HNV DH    
Sbjct: 61  PPNHDQINAMIRSYKSRSFPKHSSRNSSYNLKQFFSDRKNKILTDTSKLRHNVEDHHLDS 120

Query: 121 -SEHQLMELLDALDSKIRVANDMIEFMEADYDHLIDQAIGMDTPPSQTEDEETTQFNISD 180
            SEHQL ELL  LDSKI VA+ MI+FM+ADYDHLI+QA GMDT PS+TE E TT+ +IS+
Sbjct: 121 LSEHQLKELLATLDSKIGVADGMIQFMDADYDHLIEQAFGMDTLPSETE-EPTTECDISE 180

Query: 181 LFNEPDEFE--------------EYNVEGFQSLLEDKFLETLVQNNPIPDFDFD 214
              EPDEFE              EYNVEGFQSLLED+FL TLV NNP   F FD
Sbjct: 181 FLIEPDEFEYNVEGFQSPLEDEFEYNVEGFQSLLEDEFLGTLVLNNP---FKFD 230

BLAST of CSPI03G20310 vs. NCBI nr
Match: XP_038887166.1 (MADS-box protein SVP-like [Benincasa hispida])

HSP 1 Score: 232.3 bits (591), Expect = 4.1e-57
Identity = 133/203 (65.52%), Postives = 155/203 (76.35%), Query Frame = 0

Query: 1   MGRGILSLKLIPNPKSRRTTFLKRKKSLIKKAYELSTLCDVQTCLFIASDCDPSTHFETW 60
           MGRG L+LKLIPN KSRRTTF+KRKKSL+KKAYELSTLCDVQTC+FIASDCDPSTHFETW
Sbjct: 1   MGRGTLNLKLIPNLKSRRTTFVKRKKSLMKKAYELSTLCDVQTCVFIASDCDPSTHFETW 60

Query: 61  PPNHHQIHQMIRSYKSHSFTK-PNSSYDLNRFFSDRKNKILTNTSKLLHNV-------VD 120
           PPNH QIH MIRSYKSHSF K   +SY+L++FFS R NKI T TSKL HNV       +D
Sbjct: 61  PPNHDQIHSMIRSYKSHSFPKLSRNSYNLHQFFSHRMNKIATETSKLRHNVDKFKDRHLD 120

Query: 121 HQSEHQLMELLDALDSKIRVANDMIEFMEADYDHLIDQAIG---MDTP---PSQTEDEET 180
             SEHQL ELL +LDSKI V + MI+FMEADYDHLI++  G   +DTP   PS+T  +ET
Sbjct: 121 SLSEHQLEELLASLDSKIGVVDGMIQFMEADYDHLIEEVSGFSSIDTPSFLPSET--KET 180

Query: 181 TQFNISDLFNEPDEFEEYNVEGF 190
            + +I++     +   EYNVE F
Sbjct: 181 PELDITEWLIGTNGL-EYNVEEF 200

BLAST of CSPI03G20310 vs. NCBI nr
Match: XP_023526169.1 (agamous-like MADS-box protein AGL6 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 193.0 bits (489), Expect = 2.7e-45
Identity = 116/191 (60.73%), Postives = 134/191 (70.16%), Query Frame = 0

Query: 1   MGRGILSLKLIPNPKSRRTTFLKRKKSLIKKAYELSTLCDVQTCLFIASDCDPSTHFETW 60
           MGRG LSLKLI NPKSRRTTF KRKKSL+KKAYELSTLCDVQTC+ IASD      F+TW
Sbjct: 1   MGRGTLSLKLISNPKSRRTTFQKRKKSLMKKAYELSTLCDVQTCVVIASD-----EFQTW 60

Query: 61  PPNHHQIHQMIRSYKSHSFTK--PNSSYDLNRFFSDRKNKILTNTSKLLHNV-------- 120
           P + +Q+  MIRSYKS SF K    SSYDLN+FFSDRKNKI+T TSKL   V        
Sbjct: 61  PSDRNQVEAMIRSYKSQSFCKHSAKSSYDLNQFFSDRKNKIVTETSKLRDKVQKSKDTNS 120

Query: 121 ---VDHQSEHQLMELLDALDSKIRVANDMIEFMEADYDHLIDQAIGMDTPPSQT----ED 175
              +D  SEHQL  LL  LDSKI VA+ MIEFMEADYD+LI+ A+ + +   QT    E 
Sbjct: 121 DHCLDSLSEHQLKVLLATLDSKIGVADSMIEFMEADYDYLIEGAVEISSIDKQTLSPLET 180

BLAST of CSPI03G20310 vs. TAIR 10
Match: AT5G58890.1 (AGAMOUS-like 82 )

HSP 1 Score: 62.4 bits (150), Expect = 5.2e-10
Identity = 60/194 (30.93%), Postives = 93/194 (47.94%), Query Frame = 0

Query: 5   ILSLKLIPNPKSRRTTFLKRKKSLIKKAYELSTLCDVQTCLFIASDCDPS----THFETW 64
           ++ L+ I N K+R TT+ KRK SL KKA E STLC V+TCL +      +    +  E W
Sbjct: 5   VVDLQRIANDKTRITTYKKRKASLYKKAQEFSTLCGVETCLIVYGPTKATDVVISEPEIW 64

Query: 65  PPNHHQIHQMIRSYKSHSFTKPNSSYDLNRFFSD--RKNKILT-------NTSKLLHNVV 124
           P +  ++  +IR YK    T      ++  F +D  + N+++T       N        +
Sbjct: 65  PKDETKVRAIIRKYKDTVSTSCRKETNVETFVNDVGKGNEVVTKKRVKRENKYSSWEEKL 124

Query: 125 DHQSEHQLMELLDALDSKIRVANDMIEFMEADYDHLIDQAIGMDTP-PSQTEDEETTQFN 184
           D  S  QL  +  A+DSK+   N+ +   E     +  QA  MDTP P    D+   QF 
Sbjct: 125 DKCSREQLHGIFCAVDSKL---NEAVTRQERSMFRVNHQA--MDTPFPQNLMDQ---QF- 184

BLAST of CSPI03G20310 vs. TAIR 10
Match: AT2G03710.1 (K-box region and MADS-box transcription factor family protein )

HSP 1 Score: 58.9 bits (141), Expect = 5.8e-09
Identity = 44/129 (34.11%), Postives = 64/129 (49.61%), Query Frame = 0

Query: 1   MGRGILSLKLIPNPKSRRTTFLKRKKSLIKKAYELSTLCDVQTCLFIASDCDPSTHFETW 60
           MGRG + LK I N  +R+ TF KR+  L+KKAYELS LCD +  L I S+      F + 
Sbjct: 1   MGRGKVELKRIENKINRQVTFAKRRNGLLKKAYELSVLCDAEIALLIFSNRGKLYEFCSS 60

Query: 61  PPNHHQIHQMIRSYKSHSFT--KPNSSYDLNRFFSDRKNKILTNTSKLLHNVVDHQSEHQ 120
           P     + + +  Y+ HS+    PN S    +   D+    L   S++   ++ H   H 
Sbjct: 61  PSG---MARTVDKYRKHSYATMDPNQS---AKDLQDKYQDYLKLKSRV--EILQHSQRHL 120

Query: 121 LMELLDALD 128
           L E L  +D
Sbjct: 121 LGEELSEMD 121

BLAST of CSPI03G20310 vs. TAIR 10
Match: AT2G03710.2 (K-box region and MADS-box transcription factor family protein )

HSP 1 Score: 58.9 bits (141), Expect = 5.8e-09
Identity = 44/129 (34.11%), Postives = 64/129 (49.61%), Query Frame = 0

Query: 1   MGRGILSLKLIPNPKSRRTTFLKRKKSLIKKAYELSTLCDVQTCLFIASDCDPSTHFETW 60
           MGRG + LK I N  +R+ TF KR+  L+KKAYELS LCD +  L I S+      F + 
Sbjct: 1   MGRGKVELKRIENKINRQVTFAKRRNGLLKKAYELSVLCDAEIALLIFSNRGKLYEFCSS 60

Query: 61  PPNHHQIHQMIRSYKSHSFT--KPNSSYDLNRFFSDRKNKILTNTSKLLHNVVDHQSEHQ 120
           P     + + +  Y+ HS+    PN S    +   D+    L   S++   ++ H   H 
Sbjct: 61  PSG---MARTVDKYRKHSYATMDPNQS---AKDLQDKYQDYLKLKSRV--EILQHSQRHL 120

Query: 121 LMELLDALD 128
           L E L  +D
Sbjct: 121 LGEELSEMD 121

BLAST of CSPI03G20310 vs. TAIR 10
Match: AT2G03710.3 (K-box region and MADS-box transcription factor family protein )

HSP 1 Score: 58.9 bits (141), Expect = 5.8e-09
Identity = 44/129 (34.11%), Postives = 64/129 (49.61%), Query Frame = 0

Query: 1   MGRGILSLKLIPNPKSRRTTFLKRKKSLIKKAYELSTLCDVQTCLFIASDCDPSTHFETW 60
           MGRG + LK I N  +R+ TF KR+  L+KKAYELS LCD +  L I S+      F + 
Sbjct: 1   MGRGKVELKRIENKINRQVTFAKRRNGLLKKAYELSVLCDAEIALLIFSNRGKLYEFCSS 60

Query: 61  PPNHHQIHQMIRSYKSHSFT--KPNSSYDLNRFFSDRKNKILTNTSKLLHNVVDHQSEHQ 120
           P     + + +  Y+ HS+    PN S    +   D+    L   S++   ++ H   H 
Sbjct: 61  PSG---MARTVDKYRKHSYATMDPNQS---AKDLQDKYQDYLKLKSRV--EILQHSQRHL 120

Query: 121 LMELLDALD 128
           L E L  +D
Sbjct: 121 LGEELSEMD 121

BLAST of CSPI03G20310 vs. TAIR 10
Match: AT5G55690.1 (MADS-box transcription factor family protein )

HSP 1 Score: 54.3 bits (129), Expect = 1.4e-07
Identity = 38/143 (26.57%), Postives = 66/143 (46.15%), Query Frame = 0

Query: 1   MGRGILSLKLIPNPKSRRTTFLKRKKSLIKKAYELSTLCDVQTCLFIASDCDPSTHF--- 60
           MGR ++ +  I N K+R TT+ KRK  L KKA E STLC V TC+ +             
Sbjct: 1   MGRKMVKMTRITNEKTRITTYKKRKACLYKKASEFSTLCGVDTCVIVYGPSRAGDEMVME 60

Query: 61  -ETWPPNHHQIHQMIRSYKSHSFTKPNSSYDLNRFFSDRKNKILTNTSKLLH----NVVD 120
            E WP +  ++ +++  Y+  + +    +Y +         K+   T    +      +D
Sbjct: 61  PELWPKDGSKVREILTKYRDTASSSCTKTYTVQECLEKNNTKVEKPTIATKYPTWDKKLD 120

Query: 121 HQSEHQLMELLDALDSKIRVAND 136
             S + L  +  A+++KI+ A +
Sbjct: 121 QCSLNDLYAVFMAVENKIQEATN 143

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9FIM07.3e-0930.93Agamous-like MADS-box protein AGL82 OS=Arabidopsis thaliana OX=3702 GN=AGL82 PE=... [more]
P293838.1e-0834.11Agamous-like MADS-box protein AGL3 OS=Arabidopsis thaliana OX=3702 GN=AGL3 PE=1 ... [more]
Q386941.8e-0756.00Agamous-like MADS-box protein AGL9 homolog OS=Aranda deborah OX=29714 PE=2 SV=1[more]
F6I4576.9e-0727.71Agamous-like MADS-box protein AGL11 OS=Vitis vinifera OX=29760 GN=AGL11 PE=2 SV=... [more]
A0A217EJJ06.9e-0727.71Agamous-like MADS-box protein AGL11 OS=Vitis vinifera OX=29760 GN=AGL11 PE=3 SV=... [more]
Match NameE-valueIdentityDescription
A0A0A0LAL91.5e-11899.54MADS-box domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G258140 PE... [more]
A0A6J1F3703.9e-4556.67agamous-like MADS-box protein AGL6 OS=Cucurbita moschata OX=3662 GN=LOC111441717... [more]
A0A5D3DXK11.3e-3787.63Agamous-like MADS-box protein AGL3 OS=Cucumis melo var. makuwa OX=1194695 GN=E56... [more]
A0A5A7U2J61.3e-2465.66Agamous-like MADS-box protein AGL3 OS=Cucumis melo var. makuwa OX=1194695 GN=E56... [more]
A0A6J1J5U64.2e-2338.86agamous-like MADS-box protein AGL14 OS=Cucurbita maxima OX=3661 GN=LOC111482037 ... [more]
Match NameE-valueIdentityDescription
XP_004145650.13.2e-11899.54agamous-like MADS-box protein AGL82 [Cucumis sativus][more]
KAE8650565.13.2e-11899.54hypothetical protein Csa_011366 [Cucumis sativus][more]
XP_038877825.11.3e-7671.37agamous-like MADS-box protein MADS1 [Benincasa hispida][more]
XP_038887166.14.1e-5765.52MADS-box protein SVP-like [Benincasa hispida][more]
XP_023526169.12.7e-4560.73agamous-like MADS-box protein AGL6 [Cucurbita pepo subsp. pepo][more]
Match NameE-valueIdentityDescription
AT5G58890.15.2e-1030.93AGAMOUS-like 82 [more]
AT2G03710.15.8e-0934.11K-box region and MADS-box transcription factor family protein [more]
AT2G03710.25.8e-0934.11K-box region and MADS-box transcription factor family protein [more]
AT2G03710.35.8e-0934.11K-box region and MADS-box transcription factor family protein [more]
AT5G55690.11.4e-0726.57MADS-box transcription factor family protein [more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (PI 183967) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 123..143
NoneNo IPR availablePANTHERPTHR11945:SF176AGAMOUS-LIKE MADS-BOX PROTEIN AGL82coord: 3..169
NoneNo IPR availablePANTHERPTHR11945MADS BOX PROTEINcoord: 3..169
IPR002100Transcription factor, MADS-boxPRINTSPR00404MADSDOMAINcoord: 38..59
score: 26.0
coord: 23..38
score: 64.75
coord: 3..23
score: 40.64
IPR002100Transcription factor, MADS-boxSMARTSM00432madsneu2coord: 1..62
e-value: 5.1E-16
score: 69.2
IPR002100Transcription factor, MADS-boxPFAMPF00319SRF-TFcoord: 11..50
e-value: 1.3E-15
score: 56.7
IPR002100Transcription factor, MADS-boxPROSITEPS50066MADS_BOX_2coord: 1..61
score: 18.193771
IPR036879Transcription factor, MADS-box superfamilyGENE3D3.40.1810.10coord: 14..100
e-value: 6.7E-17
score: 63.4
IPR036879Transcription factor, MADS-box superfamilySUPERFAMILY55455SRF-likecoord: 1..81
IPR033897MADS SRF-likeCDDcd00266MADS_SRF_likecoord: 2..68
e-value: 1.56874E-21
score: 82.6931

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CSPI03G20310.1CSPI03G20310.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0045944 positive regulation of transcription by RNA polymerase II
cellular_component GO:0005634 nucleus
molecular_function GO:0000981 DNA-binding transcription factor activity, RNA polymerase II-specific
molecular_function GO:0046983 protein dimerization activity
molecular_function GO:0000978 RNA polymerase II cis-regulatory region sequence-specific DNA binding
molecular_function GO:0000987 cis-regulatory region sequence-specific DNA binding
molecular_function GO:0003677 DNA binding