CSPI03G07140 (gene) Cucumber (PI 183967) v1

Overview
NameCSPI03G07140
Typegene
OrganismCucumis sativus var. hardwickii cv. PI 183967 (Cucumber (PI 183967) v1)
DescriptionL-gulonolactone oxidase
LocationChr3: 6368282 .. 6371153 (-)
RNA-Seq ExpressionCSPI03G07140
SyntenyCSPI03G07140
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
GTTTGGGCAAGAGAGAAGTTTGGGGGATATTAATGGATCCCAGAAACTTTAGTAAATAAACTACTTTTCGTTGGTAAGAGAAGGGGGTGTCCTGAAGCTTAGAAAACTTAAGTCCTACACTATGCGTCGGTGGTCGTGGCGGTCTTATTGCGTTGTCTCCTGCCCGAGCTCGGTGGTTTTCATGTTTCTTGTTCTTGTTTCTGAATGCAGTCCACCGGGGGATCCCATCAAATGCGTATCCGTATCCCCAAATAAATACGCCAATTGTACCATCACAAACTTCTACGGTTCGTTTCCGGACCGAAGCATCTGCCGTGTGGCGGAGGTGATATACCCAACCACAGAGGCGGAGGTGATGTTGGCAGTGGCAGCGGCGACGAGAGGCTTGAGGAAAATGAAGGTGGGGACTCGGTTCTCTCACAGCATTCCGAAGCTGGCATGTCCGGAGGGGGAGGAGGGGGTGGTTATAAGCACAAAGTACTTGAACAGGACTGTAAAAGTGGACGTTGAGGGAATGAGCATAAGCGTGGAAGGGGGAGCCACGCTGAGGCAGATCATAGAGGATGCTGCGACGGCTGGGCTGGCTCTGCCTAGTACTCCTTACTGGTGGGGGCTCACCATCGGAGGACTCCTAAGCACCGGGGCACATGGGAGCTCACTATGGGGCAAAGGCAGCGCAGTCCACGACTACGTTGTGGCCGTTCAAATGGTCAGTCCTGGTGGTCCTGAAGATGGGTACGCCAAACAACGGACCATTCTACCTGATGACCAAAACCATCTCAACGCTGCCAAAGTTTCTTTGGGAGTTCTTGGAGTTATTACACAGGTCAGCATGCATCTGCATTTTATATTACTAGTTAAAATCTTGCCATGTTACTTGCTGTGAGGGCGTATAAAATAAGTATATAAATTTAAAAAGAGTAATTGCTGGGTTTTTTTTCTTCTTCTTTTATGCATTGTTGAAATAATGATGTTGAGTAAAGGAATATGTGGGACGATACAGAGTTGTTAAACTTGTTTTGTTTTGATGAGAAATTAAAATACATAGTAATTAAAGGAATATCAAATAGACACTATTATAAAATTAAGCTATAATGTCAGCATACATGTTAGATGAGAATTGTCATGTGTGAATGTATTATTACAACCATGGTCATCCTCCATATCAATTTTCAATCGACATTATCGTTAAAAATATCTATTTTTATAAGATAAAACAATCATCATCTTTATTTTATTAAAAATAAAGTATTGACTGTATAAGTAGCTAAATAATATAAACGAGTATCCATTCGTGCACAAAGTATATTCAACTTACATACATAAATAAAAATAAAGAGAAGCAAAGTGATTAATATGGCATATCGTTATACATTCAACTTAGAAAGATAAATAAAAATAAATATAGGAAGACAAAAAAAGGAATTAATATGGCACATTGGTATGATTGAAATGAGGAAATACGTGTGTAGGTAACTCTGAAATTACAACCTATGTTCAAGCGATCCATCACATATTTGAAAAGGGATGACAAGGATTTGGGGGATGAAGTAATTAGGTTTGGGAAAAGCTATGAATTTGGAGATATGAATTGGTATCCAAGCCAACGAAAAGTTGTGTATCGGATAGACACCCGCGTTCCGTCCAATACCTCCGGTAATGGCCTCTATGACTTTATCCCATTTCGCCCCACGCTTTCAGTAGAATTGGCACTTCTTCGGGCCTCAGGTTAATATTATTTACACTTCAATTATTAATTGAGTCAAATTTTTTTTGGTTAGCCGGCCAATTAATTAATCACCTCTGAAAATATTATAATATGGACTAATAATTAATTCATTCATTATATTAGAGGAGGAGCAAGAATCAAGGCGTGATGCAGCTGCCAAGTGTTTAAGTGGAAACCTAATCACGTCCACTCTTTCACTGTTGGCTTACGGATTAACCAACAATGGTAACGATCATCATCCTTAAATTTAATTTTGTGGTTTTAGTTAGTTATATTAAACAAAATGTATAATATATATATGTCTTATATAAATATTGTAGGCATCACATTTCTGAAATATCCAGTGATCGGGTATAACAATCGACTTCAGTCCTCTGGAAGTTGCCTAGACAGCCTCAATGATTTTAGAATAACGGCATGCCCTTGGGATCCTTTGATCAAAGGAGAGTTCTTCTATGAAACATCCGTTAGCATTGAGTTGACTGTGGTCAAAAGTTTCATCGAAGATGTCCAGAAACTTGCTGAGTTGGAACCAATGGCATTCTGTGGTCTTGAGCTCTACAATGGCATCCTCATGCGATATGTTACAGCCTCCACCGCCTACTTAGGGAAGCAACAAGATAGCGTTGAGTTTGACTTAACATATTACAGAAGCAAAGACCCAATGAGTCCAAGGCTTTTCGAGGACGTGATAGAAGAGGTGGAGCAAATGGCAGTGTTCAAATATGGAGGCATTCCCCATTGGGGAAAGAACAGGAACGTGGCATTTCATCAAGTATTTCACAAATACAGAAATATAGAAAAGTTTTTGAAGATTATGAAGGAATATGATCCCCATAGGTTGTTTTCAAATGAGTGGACAGATCAAATCCTTGGAGTAGAAGGAACGGTGAATATATACAAAGATGGTTGTGCGTTGGAAGGACTTTGCATATGCTCAAAACATACTCATTGTGCCCCAAGTAAGGGCTACTTTTGTAGGGCTGGCAAAATTTACAAGGATGCTAGGGTTTGTTCTTATTTACAATCACCCAATCATACTTAATTAATTAACTACCTAACTTTGTGATATTTATATATATTTATATATATATACACACTCATACATGGCAATGAGTCATGATGAAAAAAGATACACAGTGC

mRNA sequence

GTTTGGGCAAGAGAGAAGTTTGGGGGATATTAATGGATCCCAGAAACTTTAGTAAATAAACTACTTTTCGTTGGTAAGAGAAGGGGGTGTCCTGAAGCTTAGAAAACTTAAGTCCTACACTATGCGTCGGTGGTCGTGGCGGTCTTATTGCGTTGTCTCCTGCCCGAGCTCGGTGGTTTTCATGTTTCTTGTTCTTGTTTCTGAATGCAGTCCACCGGGGGATCCCATCAAATGCGTATCCGTATCCCCAAATAAATACGCCAATTGTACCATCACAAACTTCTACGGTTCGTTTCCGGACCGAAGCATCTGCCGTGTGGCGGAGGTGATATACCCAACCACAGAGGCGGAGGTGATGTTGGCAGTGGCAGCGGCGACGAGAGGCTTGAGGAAAATGAAGGTGGGGACTCGGTTCTCTCACAGCATTCCGAAGCTGGCATGTCCGGAGGGGGAGGAGGGGGTGGTTATAAGCACAAAGTACTTGAACAGGACTGTAAAAGTGGACGTTGAGGGAATGAGCATAAGCGTGGAAGGGGGAGCCACGCTGAGGCAGATCATAGAGGATGCTGCGACGGCTGGGCTGGCTCTGCCTAGTACTCCTTACTGGTGGGGGCTCACCATCGGAGGACTCCTAAGCACCGGGGCACATGGGAGCTCACTATGGGGCAAAGGCAGCGCAGTCCACGACTACGTTGTGGCCGTTCAAATGGTCAGTCCTGGTGGTCCTGAAGATGGGTACGCCAAACAACGGACCATTCTACCTGATGACCAAAACCATCTCAACGCTGCCAAAGTTTCTTTGGGAGTTCTTGGAGTTATTACACAGGTAACTCTGAAATTACAACCTATGTTCAAGCGATCCATCACATATTTGAAAAGGGATGACAAGGATTTGGGGGATGAAGTAATTAGGTTTGGGAAAAGCTATGAATTTGGAGATATGAATTGGTATCCAAGCCAACGAAAAGTTGTGTATCGGATAGACACCCGCGTTCCGTCCAATACCTCCGGTAATGGCCTCTATGACTTTATCCCATTTCGCCCCACGCTTTCAGTAGAATTGGCACTTCTTCGGGCCTCAGAGGAGGAGCAAGAATCAAGGCGTGATGCAGCTGCCAAGTGTTTAAGTGGAAACCTAATCACGTCCACTCTTTCACTGTTGGCTTACGGATTAACCAACAATGGCATCACATTTCTGAAATATCCAGTGATCGGGTATAACAATCGACTTCAGTCCTCTGGAAGTTGCCTAGACAGCCTCAATGATTTTAGAATAACGGCATGCCCTTGGGATCCTTTGATCAAAGGAGAGTTCTTCTATGAAACATCCGTTAGCATTGAGTTGACTGTGGTCAAAAGTTTCATCGAAGATGTCCAGAAACTTGCTGAGTTGGAACCAATGGCATTCTGTGGTCTTGAGCTCTACAATGGCATCCTCATGCGATATGTTACAGCCTCCACCGCCTACTTAGGGAAGCAACAAGATAGCGTTGAGTTTGACTTAACATATTACAGAAGCAAAGACCCAATGAGTCCAAGGCTTTTCGAGGACGTGATAGAAGAGGTGGAGCAAATGGCAGTGTTCAAATATGGAGGCATTCCCCATTGGGGAAAGAACAGGAACGTGGCATTTCATCAAGTATTTCACAAATACAGAAATATAGAAAAGTTTTTGAAGATTATGAAGGAATATGATCCCCATAGGTTGTTTTCAAATGAGTGGACAGATCAAATCCTTGGAGTAGAAGGAACGGTGAATATATACAAAGATGGTTGTGCGTTGGAAGGACTTTGCATATGCTCAAAACATACTCATTGTGCCCCAAGTAAGGGCTACTTTTGTAGGGCTGGCAAAATTTACAAGGATGCTAGGGTTTGTTCTTATTTACAATCACCCAATCATACTTAATTAATTAACTACCTAACTTTGTGATATTTATATATATTTATATATATATACACACTCATACATGGCAATGAGTCATGATGAAAAAAGATACACAGTGC

Coding sequence (CDS)

ATGCGTCGGTGGTCGTGGCGGTCTTATTGCGTTGTCTCCTGCCCGAGCTCGGTGGTTTTCATGTTTCTTGTTCTTGTTTCTGAATGCAGTCCACCGGGGGATCCCATCAAATGCGTATCCGTATCCCCAAATAAATACGCCAATTGTACCATCACAAACTTCTACGGTTCGTTTCCGGACCGAAGCATCTGCCGTGTGGCGGAGGTGATATACCCAACCACAGAGGCGGAGGTGATGTTGGCAGTGGCAGCGGCGACGAGAGGCTTGAGGAAAATGAAGGTGGGGACTCGGTTCTCTCACAGCATTCCGAAGCTGGCATGTCCGGAGGGGGAGGAGGGGGTGGTTATAAGCACAAAGTACTTGAACAGGACTGTAAAAGTGGACGTTGAGGGAATGAGCATAAGCGTGGAAGGGGGAGCCACGCTGAGGCAGATCATAGAGGATGCTGCGACGGCTGGGCTGGCTCTGCCTAGTACTCCTTACTGGTGGGGGCTCACCATCGGAGGACTCCTAAGCACCGGGGCACATGGGAGCTCACTATGGGGCAAAGGCAGCGCAGTCCACGACTACGTTGTGGCCGTTCAAATGGTCAGTCCTGGTGGTCCTGAAGATGGGTACGCCAAACAACGGACCATTCTACCTGATGACCAAAACCATCTCAACGCTGCCAAAGTTTCTTTGGGAGTTCTTGGAGTTATTACACAGGTAACTCTGAAATTACAACCTATGTTCAAGCGATCCATCACATATTTGAAAAGGGATGACAAGGATTTGGGGGATGAAGTAATTAGGTTTGGGAAAAGCTATGAATTTGGAGATATGAATTGGTATCCAAGCCAACGAAAAGTTGTGTATCGGATAGACACCCGCGTTCCGTCCAATACCTCCGGTAATGGCCTCTATGACTTTATCCCATTTCGCCCCACGCTTTCAGTAGAATTGGCACTTCTTCGGGCCTCAGAGGAGGAGCAAGAATCAAGGCGTGATGCAGCTGCCAAGTGTTTAAGTGGAAACCTAATCACGTCCACTCTTTCACTGTTGGCTTACGGATTAACCAACAATGGCATCACATTTCTGAAATATCCAGTGATCGGGTATAACAATCGACTTCAGTCCTCTGGAAGTTGCCTAGACAGCCTCAATGATTTTAGAATAACGGCATGCCCTTGGGATCCTTTGATCAAAGGAGAGTTCTTCTATGAAACATCCGTTAGCATTGAGTTGACTGTGGTCAAAAGTTTCATCGAAGATGTCCAGAAACTTGCTGAGTTGGAACCAATGGCATTCTGTGGTCTTGAGCTCTACAATGGCATCCTCATGCGATATGTTACAGCCTCCACCGCCTACTTAGGGAAGCAACAAGATAGCGTTGAGTTTGACTTAACATATTACAGAAGCAAAGACCCAATGAGTCCAAGGCTTTTCGAGGACGTGATAGAAGAGGTGGAGCAAATGGCAGTGTTCAAATATGGAGGCATTCCCCATTGGGGAAAGAACAGGAACGTGGCATTTCATCAAGTATTTCACAAATACAGAAATATAGAAAAGTTTTTGAAGATTATGAAGGAATATGATCCCCATAGGTTGTTTTCAAATGAGTGGACAGATCAAATCCTTGGAGTAGAAGGAACGGTGAATATATACAAAGATGGTTGTGCGTTGGAAGGACTTTGCATATGCTCAAAACATACTCATTGTGCCCCAAGTAAGGGCTACTTTTGTAGGGCTGGCAAAATTTACAAGGATGCTAGGGTTTGTTCTTATTTACAATCACCCAATCATACTTAA

Protein sequence

MRRWSWRSYCVVSCPSSVVFMFLVLVSECSPPGDPIKCVSVSPNKYANCTITNFYGSFPDRSICRVAEVIYPTTEAEVMLAVAAATRGLRKMKVGTRFSHSIPKLACPEGEEGVVISTKYLNRTVKVDVEGMSISVEGGATLRQIIEDAATAGLALPSTPYWWGLTIGGLLSTGAHGSSLWGKGSAVHDYVVAVQMVSPGGPEDGYAKQRTILPDDQNHLNAAKVSLGVLGVITQVTLKLQPMFKRSITYLKRDDKDLGDEVIRFGKSYEFGDMNWYPSQRKVVYRIDTRVPSNTSGNGLYDFIPFRPTLSVELALLRASEEEQESRRDAAAKCLSGNLITSTLSLLAYGLTNNGITFLKYPVIGYNNRLQSSGSCLDSLNDFRITACPWDPLIKGEFFYETSVSIELTVVKSFIEDVQKLAELEPMAFCGLELYNGILMRYVTASTAYLGKQQDSVEFDLTYYRSKDPMSPRLFEDVIEEVEQMAVFKYGGIPHWGKNRNVAFHQVFHKYRNIEKFLKIMKEYDPHRLFSNEWTDQILGVEGTVNIYKDGCALEGLCICSKHTHCAPSKGYFCRAGKIYKDARVCSYLQSPNHT*
Homology
[Close]
BLAST of CSPI03G07140 vs. ExPASy Swiss-Prot
Match: O81032 (Probable L-gulonolactone oxidase 6 OS=Arabidopsis thaliana OX=3702 GN=GULLO6 PE=3 SV=1)

HSP 1 Score: 759.6 bits (1960), Expect = 2.6e-218
Identity = 362/583 (62.09%), Postives = 447/583 (76.67%), Query Frame = 0 

Query: 6   WRSYCVVSCPSSVVFMFLVLVSECSPPGDPIKCVSVSPNKYANCTITNFYGSFPDRSICR 65
           WR+  VV C S++VF  +      +PP DP+KCVS +     NCT+TN YG+FPDRS CR
Sbjct: 17  WRTIFVVHCISTLVFTTI-----STPPEDPVKCVSGN----TNCTVTNSYGAFPDRSTCR 76

Query: 66  VAEVIYPTTEAEVMLAVAAATRGLRKMKVGTRFSHSIPKLACPEGEEGVVISTKYLNRTV 125
            A V YPTTEAE++  VAAAT+  RKM+V TR+SHSI KLAC +G +G++ISTK+LN TV
Sbjct: 77  AANVAYPTTEAELISVVAAATKAGRKMRVTTRYSHSITKLACTDGTDGLLISTKFLNHTV 136

Query: 126 KVDVEGMSISVEGGATLRQIIEDAATAGLALPSTPYWWGLTIGGLLSTGAHGSSLWGKGS 185
           + D   M+++VE G TLRQ+I +AA  GLALP  PYWWGLT+GG++ TGAHGSSLWGKGS
Sbjct: 137 RTDATAMTLTVESGVTLRQLIAEAAKVGLALPYAPYWWGLTVGGMMGTGAHGSSLWGKGS 196

Query: 186 AVHDYVVAVQMVSPGGPEDGYAKQRTIL-PDDQNHLNAAKVSLGVLGVITQVTLKLQPMF 245
           AVHDYV  +++VSPG   DG+AK R +         NAAKVSLGVLGVI+QVTLKLQPMF
Sbjct: 197 AVHDYVTEIRIVSPGSVNDGFAKVRVLRETTTPKEFNAAKVSLGVLGVISQVTLKLQPMF 256

Query: 246 KRSITYLKRDDKDLGDEVIRFGKSYEFGDMNWYPSQRKVVYRIDTRVPSNTSGNGLYDFI 305
           KRS+ Y+ R+D D GD+ + FG  +EF D  W PSQ KVVYR+D RV  NTSGNGL DF+
Sbjct: 257 KRSLRYVMRNDSDFGDQAVTFGMKHEFADFIWLPSQGKVVYRMDDRVAVNTSGNGLLDFM 316

Query: 306 PFRPTLSVELALLRASEEEQESRRDAAAKCLSGNLITSTLSLLAYGLTNNGITFLKYPVI 365
           PFR  LS  LA++R+SEE QE  RDA  KC    LITSTL   +YGLTNNG+ F  YPVI
Sbjct: 317 PFRSQLSAALAIIRSSEETQERFRDANGKCAGATLITSTLFATSYGLTNNGMIFTGYPVI 376

Query: 366 GYNNRLQSSGSCLDSLNDFRITACPWDPLIKGEFFYETSVSIELTVVKSFIEDVQKLAEL 425
           G  NR+ SSGSCLDSL+D  ITACPWD  IK EFF++T+ SI LT VKSFI D++ L ++
Sbjct: 377 GSQNRMMSSGSCLDSLHDGLITACPWDSRIKSEFFHQTTFSIPLTQVKSFINDIKSLVKI 436

Query: 426 EPMAFCGLELYNGILMRYVTASTAYLGKQQDSVEFDLTYYRSKDPMSPRLFEDVIEEVEQ 485
           E  + C LELY+GILMRYVT+S AYLGK+ ++++FDLTYYR+KDP+SPRL+ED IEE+EQ
Sbjct: 437 ESKSLCVLELYDGILMRYVTSSPAYLGKETEALDFDLTYYRAKDPLSPRLYEDFIEEIEQ 496

Query: 486 MAVFKYGGIPHWGKNRNVAFHQVFHKYRNIEKFLKIMKEYDPHRLFSNEWTDQILGVEGT 545
           +A+FKY  +PHWGKNRN+AF  V  KY+N+  FLK+ + YDP  LFS+EWTDQILG++G 
Sbjct: 497 IALFKYNALPHWGKNRNLAFDGVIKKYKNVPAFLKVKESYDPMGLFSSEWTDQILGIKGN 556

Query: 546 VNIYKDGCALEGLCICSKHTHCAPSKGYFCRAGKIYKDARVCS 588
           V I KDGCALEGLC+CS+  HCAP+KGYFCR GK+YK+ARVC+
Sbjct: 557 VTIIKDGCALEGLCVCSEDAHCAPTKGYFCRPGKVYKEARVCT 590

[Close]
BLAST of CSPI03G07140 vs. ExPASy Swiss-Prot
Match: Q6NQ66 (L-gulonolactone oxidase 2 OS=Arabidopsis thaliana OX=3702 GN=GULLO2 PE=1 SV=1)

HSP 1 Score: 737.3 bits (1902), Expect = 1.4e-211
Identity = 347/559 (62.08%), Postives = 428/559 (76.57%), Query Frame = 0 

Query: 30  SPPGDPIKCVSVSPNKYANCTITNFYGSFPDRSICRVAEVIYPTTEAEVMLAVAAATRGL 89
           +PP DP+KCVS +     NCT+TN YG+FPDRS CR A V YP TEAE++  VAAAT+  
Sbjct: 28  TPPDDPVKCVSGN----TNCTVTNSYGAFPDRSTCRAANVAYPKTEAELVSVVAAATQAG 87

Query: 90  RKMKVGTRFSHSIPKLACPEGEEGVVISTKYLNRTVKVDVEGMSISVEGGATLRQIIEDA 149
           RKM+V TR+SHSI KL C +G EG+ ISTK+LN TV+ D   M+++VE G TLRQ+I +A
Sbjct: 88  RKMRVTTRYSHSITKLVCTDGTEGLFISTKFLNHTVQADATAMTMTVESGMTLRQLIVEA 147

Query: 150 ATAGLALPSTPYWWGLTIGGLLSTGAHGSSLWGKGSAVHDYVVAVQMVSPGGPEDGYAKQ 209
           A  GLALP  PYWWGLT+GG++ TGAHGSSLWGKGSAVHDYV  ++MVSPG   +G+AK 
Sbjct: 148 AKVGLALPYAPYWWGLTVGGMMGTGAHGSSLWGKGSAVHDYVTEIRMVSPGSVNEGFAKI 207

Query: 210 RTIL-PDDQNHLNAAKVSLGVLGVITQVTLKLQPMFKRSITYLKRDDKDLGDEVIRFGKS 269
           R +      N  NAAKVSLGVLGVI+QVT +LQPMFKRS+TY  R+D D  D+ + FGK 
Sbjct: 208 RILSETTTPNEFNAAKVSLGVLGVISQVTFELQPMFKRSLTYTMRNDSDFEDQAVTFGKK 267

Query: 270 YEFGDMNWYPSQRKVVYRIDTRVPSNTSGNGLYDFIPFRPTLSVELALLRASEEEQESRR 329
           +EF D  W PSQ KVVYR D RV  NTSGNGL+DF+PFR  LS  +A++R SEE QE  R
Sbjct: 268 HEFADFIWLPSQGKVVYRRDDRVAVNTSGNGLFDFLPFRSQLSAAIAIIRTSEETQERFR 327

Query: 330 DAAAKCLSGNLITSTLSLLAYGLTNNGITFLKYPVIGYNNRLQSSGSCLDSLNDFRITAC 389
           DA  KC+   +I+STL   +YGLTNNGI F  YPV+G  NR+ SSGSCLDSL D  ITAC
Sbjct: 328 DANGKCVGATIISSTLFAPSYGLTNNGIIFTGYPVVGSQNRMMSSGSCLDSLQDGLITAC 387

Query: 390 PWDPLIKGEFFYETSVSIELTVVKSFIEDVQKLAELEPMAFCGLELYNGILMRYVTASTA 449
            WD  IKGEFF++T++S+ LT VKSFI D++ L ++E  + CGLEL+ GILMRYVT+S A
Sbjct: 388 AWDSRIKGEFFHQTTLSVPLTQVKSFISDIKSLVKIEQKSLCGLELHYGILMRYVTSSPA 447

Query: 450 YLGKQQDSVEFDLTYYRSKDPMSPRLFEDVIEEVEQMAVFKYGGIPHWGKNRNVAFHQVF 509
           YLGK+ ++++FD+TYYR+KDP++PRL+ED IEE+EQ+A+FKY  +PHWGKNRN+AF  V 
Sbjct: 448 YLGKETEALDFDITYYRAKDPLTPRLYEDFIEEIEQIALFKYNALPHWGKNRNLAFDGVI 507

Query: 510 HKYRNIEKFLKIMKEYDPHRLFSNEWTDQILGVEGTVNIYKDGCALEGLCICSKHTHCAP 569
            KY N   FLK+   YDP  LFS+EWTDQILG++G  +I KDGCALEGLCICSK  HCAP
Sbjct: 508 RKYNNAPAFLKVKDSYDPKGLFSSEWTDQILGIKGNASIVKDGCALEGLCICSKDAHCAP 567

Query: 570 SKGYFCRAGKIYKDARVCS 588
           +KGY CR GK+YK+ARVC+
Sbjct: 568 AKGYLCRPGKVYKEARVCT 582

[Close]
BLAST of CSPI03G07140 vs. ExPASy Swiss-Prot
Match: O81030 (L-gulonolactone oxidase 5 OS=Arabidopsis thaliana OX=3702 GN=GULLO5 PE=1 SV=1)

HSP 1 Score: 728.0 bits (1878), Expect = 8.5e-209
Identity = 346/585 (59.15%), Postives = 435/585 (74.36%), Query Frame = 0 

Query: 6   WRSYCVVSCPSSVVFMFLVLVSECSPPGDPIKCVSVSPNKYANCTITNFYGSFPDRSICR 65
           WR+   + C  ++V   +      +PP DP+KCVS +     NC +TN  G+FPDRS CR
Sbjct: 13  WRTLLGLYCLFTLVHTVI-----STPPEDPVKCVSGN----TNCIVTNSLGAFPDRSTCR 72

Query: 66  VAEVIYPTTEAEVMLAVAAATRGLRKMKVGTRFSHSIPKLACPEGEEGVVISTKYLNRTV 125
            A V YPTTEAE++  VAAAT+  RKM+V TR+SHSIPKL C +G +G+ ISTK+LN TV
Sbjct: 73  AANVAYPTTEAELVSIVAAATKAGRKMRVTTRYSHSIPKLTCTDGNDGLFISTKFLNHTV 132

Query: 126 KVDVEGMSISVEGGATLRQIIEDAATAGLALPSTPYWWGLTIGGLLSTGAHGSSLWGKGS 185
           + D + M+++VE G TLRQ+I +AA  GLALP  PYWWG+T+GG++ TGAHGSSLWGKGS
Sbjct: 133 QADAKAMTLTVESGVTLRQLIAEAAKVGLALPYAPYWWGVTVGGMMGTGAHGSSLWGKGS 192

Query: 186 AVHDYVVAVQMVSPGGPEDGYAKQRTIL-PDDQNHLNAAKVSLGVLGVITQVTLKLQPMF 245
           AVHDYV  ++MVSPG   DG+AK R +      N  NAAKVSLGVLGVI+QVT +LQPMF
Sbjct: 193 AVHDYVTEIRMVSPGSVNDGFAKIRVLSETTTPNEFNAAKVSLGVLGVISQVTFELQPMF 252

Query: 246 KRSITYLKRDDKDLGDEVIRFGKSYEFGDMNWYPSQRKVVYRIDTRVPSNTSGNGLYDFI 305
           KRS+ Y+ R+D D  DE + FGK +EF D  W PSQ KVVYR+D RV  NT GNGLYDF 
Sbjct: 253 KRSLKYVMRNDLDFNDEALTFGKKHEFADFVWLPSQGKVVYRMDDRVAVNTLGNGLYDFF 312

Query: 306 PFRPTLSVELALLRASEEEQESRRDAAAKCLSGNLITSTLSLLAYGLTNNGITFLKYPVI 365
           PFR  LS  LA  R+SEE QE+ RDA  KC++   I+STL   +YGLTNNGITF  YPVI
Sbjct: 313 PFRSQLSAVLATTRSSEETQETLRDAHGKCVTATTISSTLFSTSYGLTNNGITFTGYPVI 372

Query: 366 GYNNRLQSSGSCLDSLNDFRITACPWDPLIKGEFFYETSVSIELTVVKSFIEDVQKLAEL 425
           G  NR+ SSGSCLD L D   +AC WD  +KG F+++T+ SI LT VKSFI D++ L ++
Sbjct: 373 GSQNRMMSSGSCLDGLEDKLTSACAWDSRVKGVFYHQTTFSIPLTQVKSFINDIKSLLKI 432

Query: 426 EPMAFCGLELYNGILMRYVTASTAYLGKQQDSVEFDLTYYRSKDPMSPRLFEDVIEEVEQ 485
           +  + CGLELY GILMRYVT+S AYLGK+ ++++FD+TYYR+ DP++PRL+ED IEE+EQ
Sbjct: 433 DSKSLCGLELYYGILMRYVTSSPAYLGKETEAIDFDITYYRANDPLTPRLYEDFIEEIEQ 492

Query: 486 MAVFKYGGIPHWGKNRNVAFHQVFHKYRNIEKFLKIMKEYDPHRLFSNEWTDQILGVEGT 545
           +A+ KY  +PHWGKNRN+AF  V  KY+N   FLK+ + YDP+ LFS+EWTDQILG++G 
Sbjct: 493 IALLKYNALPHWGKNRNLAFDGVIKKYKNAPAFLKVKESYDPNGLFSSEWTDQILGIKGN 552

Query: 546 VNIYKDGCALEGLCICSKHTHCAPSKGYFCRAGKIYKDARVCSYL 590
             I KDGCALEGLCICS   HCAPSKGY CR GK+YK+ARVC+++
Sbjct: 553 PTIVKDGCALEGLCICSDDAHCAPSKGYLCRPGKVYKEARVCTHV 588

[Close]
BLAST of CSPI03G07140 vs. ExPASy Swiss-Prot
Match: Q9C614 (Probable L-gulonolactone oxidase 1 OS=Arabidopsis thaliana OX=3702 GN=GULLO1 PE=3 SV=1)

HSP 1 Score: 712.6 bits (1838), Expect = 3.7e-204
Identity = 338/579 (58.38%), Postives = 432/579 (74.61%), Query Frame = 0 

Query: 17  SVVFMFLVLVSECSPPGDPIKCVSVSPNKYANCTITNFYGSFPDRSICRVAEVIYPTTEA 76
           S++F      +  +PP DP+KC S +      CT+TN YG+FPDRSIC  A+V YP TEA
Sbjct: 6   SLIFFCFCTFASSTPPDDPVKCESGN----NMCTVTNSYGAFPDRSICEAAKVEYPKTEA 65

Query: 77  EVMLAVAAATRGLRKMKVGTRFSHSIPKLACPEGEEGVVISTKYLNRTVKVDVEGMSISV 136
           E++  VAAATR  +K++V TR+ HSIPKL C +G++GV+ISTK+LN  V  + E  +++V
Sbjct: 66  ELVSIVAAATRAGQKVRVVTRYVHSIPKLVCTDGKDGVLISTKFLNNVVGTNPEAKTLTV 125

Query: 137 EGGATLRQIIEDAATAGLALPSTPYWWGLTIGGLLSTGAHGSSLWGKGSAVHDYVVAVQM 196
           E G TLRQ+I +AA   LALP  PYWWGLT+GGL+ TGAHGSSLWGKGSAVHDYV  ++M
Sbjct: 126 ESGVTLRQLIGEAAELELALPHAPYWWGLTVGGLMGTGAHGSSLWGKGSAVHDYVSEIRM 185

Query: 197 VSPGGPEDGYAKQRTILPD-DQNHLNAAKVSLGVLGVITQVTLKLQPMFKRSITYLKRDD 256
           VSPG   DGY K R +    D +   AAKVSLGVLGVI+QVT +LQPMFKRS+T++ ++D
Sbjct: 186 VSPGLASDGYVKVRVLSETIDPDEFRAAKVSLGVLGVISQVTFQLQPMFKRSLTFVMQND 245

Query: 257 KDLGDEVIRFGKSYEFGDMNWYPSQRKVVYRIDTRVPSNTSGNGLYDFIPFRPTLSVELA 316
            D GD+ + FG+ +EF D  W PSQ KVVYR+D RVP NTSGNGL+DF PFRP LSV LA
Sbjct: 246 SDFGDQAVTFGEKHEFADFLWLPSQGKVVYRMDDRVPVNTSGNGLFDFFPFRPQLSVALA 305

Query: 317 LLRASEEEQESRRDAAAKCLSGNLITSTLSLLAYGLTNNGITFLKYPVIGYNNRLQSSGS 376
           ++R+ EE +ES  DA  KC     ITS L  ++YG+TNNG+ F  YPVIG  N + SSG+
Sbjct: 306 IIRSLEESEESSGDANDKCARAEQITSFLFSISYGVTNNGMEFTGYPVIGKQNHMMSSGT 365

Query: 377 CLDSLNDFRITACPWDPLIKGEFFYETSVSIELTVVKSFIEDVQKLAELEPMAFCGLELY 436
           CLDS  D  IT+CPWDP IKG+FF++T+ SI LT VK FI D++ L ++EP + C LE  
Sbjct: 366 CLDSHQDGLITSCPWDPRIKGQFFHQTAFSIPLTRVKGFINDIKALVKIEPKSLCALERS 425

Query: 437 NGILMRYVTASTAYLGKQQDSVEFDLTYYRSK-DPMSPRLFEDVIEEVEQMAVFKYGGIP 496
           NGIL+RYVT+S A+LGK++ +++FDLTYYRSK DP++PRL+ED IEE+EQMA+FKY  +P
Sbjct: 426 NGILIRYVTSSPAFLGKEEKALDFDLTYYRSKDDPLAPRLYEDFIEEIEQMAIFKYNALP 485

Query: 497 HWGKNRNVAFHQVFHKYRNIEKFLKIMKEYDPHRLFSNEWTDQILGVEGTVNIYKDGCAL 556
           HWGKNRN+AF  V  KY+N   FLK+ + +DP  LFS EWT+QILG++G V I K+GCAL
Sbjct: 486 HWGKNRNLAFDGVIRKYKNANTFLKVKERFDPLGLFSTEWTNQILGLKGNVTIVKEGCAL 545

Query: 557 EGLCICSKHTHCAPSKGYFCRAGKIYKDARVCSYLQSPN 594
           EGLC+CS   HCAP KGY CR GK+Y  ARVC++++S N
Sbjct: 546 EGLCVCSDDAHCAPKKGYLCRPGKVYTKARVCTHVKSVN 580

[Close]
BLAST of CSPI03G07140 vs. ExPASy Swiss-Prot
Match: Q9FM82 (Probable L-gulonolactone oxidase 4 OS=Arabidopsis thaliana OX=3702 GN=GULLO4 PE=2 SV=1)

HSP 1 Score: 709.1 bits (1829), Expect = 4.1e-203
Identity = 335/579 (57.86%), Postives = 435/579 (75.13%), Query Frame = 0 

Query: 17  SVVFMFLVLVSECSPPGDPIKCVSVSPNKYAN--CTITNFYGSFPDRSICRVAEVIYPTT 76
           S++F F    S  SPP DP+ C      ++ N  CT+TN YG+FPDRSIC+ A+V YP T
Sbjct: 6   SLIFCFFTFASS-SPPDDPVNC------EFGNTMCTVTNSYGAFPDRSICKAAKVEYPRT 65

Query: 77  EAEVMLAVAAATRGLRKMKVGTRFSHSIPKLACPEGEEGVVISTKYLNRTVKVDVEGMSI 136
           EAE++  VAAATR  +KM+V TR+SHS PKL C +G++G +ISTK+LN  V  + E  ++
Sbjct: 66  EAELVSIVAAATRAGQKMRVVTRYSHSFPKLVCTDGKDGTLISTKFLNHVVTTNPEAKTL 125

Query: 137 SVEGGATLRQIIEDAATAGLALPSTPYWWGLTIGGLLSTGAHGSSLWGKGSAVHDYVVAV 196
           +VE G TLRQ+IE+AA   LALP  PYWWGLT+GG++ TGAHGSSLWGKGSAVHDYV  +
Sbjct: 126 TVESGVTLRQLIEEAAKFDLALPYAPYWWGLTVGGMMGTGAHGSSLWGKGSAVHDYVTEI 185

Query: 197 QMVSPGGPEDGYAKQRTILPD-DQNHLNAAKVSLGVLGVITQVTLKLQPMFKRSITYLKR 256
           ++VSPG   DGY K + +    D     AAKVSLGVLGVI+QVT +LQPMFKRS+ Y+ R
Sbjct: 186 RLVSPGLASDGYVKVQVLSETIDPEEFRAAKVSLGVLGVISQVTFELQPMFKRSLNYVMR 245

Query: 257 DDKDLGDEVIRFGKSYEFGDMNWYPSQRKVVYRIDTRVPSNTSGNGLYDFIPFRPTLSVE 316
           +D D GD+ + FG+ +EF D  W PSQ KVVYR+D RVP NTSG+GL++F PFR  LS+ 
Sbjct: 246 NDSDFGDQAVSFGERHEFADFLWLPSQGKVVYRMDGRVPLNTSGDGLFEFFPFRSQLSLV 305

Query: 317 LALLRASEEEQESRRDAAAKCLSGNLITSTLSLLAYGLTNNGITFLKYPVIGYNNRLQSS 376
           LA+ R+ EE +ES  DA  KC+   L++S++ L++YG+TNNG+ F  YPVIG  N + SS
Sbjct: 306 LAIDRSLEESEESLEDANMKCVRAKLVSSSMFLMSYGVTNNGLIFTGYPVIGMQNHMMSS 365

Query: 377 GSCLDSLNDFRITACPWDPLIKGEFFYETSVSIELTVVKSFIEDVQKLAELEPMAFCGLE 436
           GSCLDS  D  ITACPWDP IKG+FF++T+ S+ LT VKSFI D++ L ++EP + C LE
Sbjct: 366 GSCLDSRQDGLITACPWDPRIKGQFFHQTTFSVSLTNVKSFINDIKALVKIEPKSLCVLE 425

Query: 437 LYNGILMRYVTASTAYLGKQQDSVEFDLTYYRSK-DPMSPRLFEDVIEEVEQMAVFKYGG 496
             NGIL+RYVT+S A+LGK++ +++FDLTYYRSK DP++PRL+ED IEE+EQMA+FKY  
Sbjct: 426 GSNGILIRYVTSSLAFLGKEEKALDFDLTYYRSKNDPLAPRLYEDYIEEIEQMAIFKYNA 485

Query: 497 IPHWGKNRNVAFHQVFHKYRNIEKFLKIMKEYDPHRLFSNEWTDQILGVEGTVNIYKDGC 556
           +PHWGKNRN+AF     KY+N   FLK+ +++D   LFS EWTDQILG++G V I K GC
Sbjct: 486 LPHWGKNRNLAFDGAIRKYKNANAFLKVKEKFDSLGLFSTEWTDQILGLKGNVTIVKQGC 545

Query: 557 ALEGLCICSKHTHCAPSKGYFCRAGKIYKDARVCSYLQS 592
           ALEGLCICS+ +HCAP+KGY CR GK+Y++ARVC+ + S
Sbjct: 546 ALEGLCICSEDSHCAPTKGYLCRPGKVYREARVCTRVSS 577

[Close]
BLAST of CSPI03G07140 vs. ExPASy TrEMBL
Match: A0A0A0L3K4 (L-gulonolactone oxidase OS=Cucumis sativus OX=3659 GN=Csa_3G116800 PE=3 SV=1)

HSP 1 Score: 1206.0 bits (3119), Expect = 0.0e+00
Identity = 593/612 (96.90%), Postives = 594/612 (97.06%), Query Frame = 0 

Query: 1   MRRWSWRSYCVVSCPSSVVFMFLVLVSECSPPGDPIKCVSVSPNKYANCTITNFYGSFPD 60
           MRRWSWRSYCVVSCPSSVVFMFLVLVSECSPPGDPIKCVSVSPNKYANCTITNFYGSFPD
Sbjct: 1   MRRWSWRSYCVVSCPSSVVFMFLVLVSECSPPGDPIKCVSVSPNKYANCTITNFYGSFPD 60

Query: 61  RSICRVAEVIYPTTEAEVMLAVAAATRGLRKMKVGTRFSHSIPKLACPEGEEGVVISTKY 120
           RSICRVAEVIYPTTEAEVMLAVAAATRGLRKMKVGTRFSHSIPKLACPEGEEGVVISTKY
Sbjct: 61  RSICRVAEVIYPTTEAEVMLAVAAATRGLRKMKVGTRFSHSIPKLACPEGEEGVVISTKY 120

Query: 121 LNRTVKVDVEGMSISVEGGATLRQIIEDAATAGLALPSTPYWWGLTIGGLLSTGAHGSSL 180
           LNRTVKVDVEGMSISVEGGATLRQIIEDAATAGLALPSTPYWWGLTIGGLLSTGAHGSSL
Sbjct: 121 LNRTVKVDVEGMSISVEGGATLRQIIEDAATAGLALPSTPYWWGLTIGGLLSTGAHGSSL 180

Query: 181 WGKGSAVHDYVVAVQMVSPGGPEDGYAKQRTILPDDQNHLNAAKVSLGVLGVITQVTLKL 240
           WGKGSAVHDYVVAVQMVSPGGPEDGYAKQRTILPDDQNHLNAAKVSLGVLGVITQVTLKL
Sbjct: 181 WGKGSAVHDYVVAVQMVSPGGPEDGYAKQRTILPDDQNHLNAAKVSLGVLGVITQVTLKL 240

Query: 241 QPMFKRSITYLKRDDKDLGDEVIRFGKSYEFGDMNWYPSQRKVVYRIDTRVPSNTSGNGL 300
           QPMFKRSITYLKRDDKDLGDEVIRFGKSYEFGDM+WYPSQRKVVYRIDTRVPSNTSGNGL
Sbjct: 241 QPMFKRSITYLKRDDKDLGDEVIRFGKSYEFGDMSWYPSQRKVVYRIDTRVPSNTSGNGL 300

Query: 301 YDFIPFRPTLSVELALLRASEEEQESRRDAAAKCLSGNLITSTLSLLAYGLTNN------ 360
           YDFIPFRPTLSVELALLRASEEEQESRRDAAAKCLSGNLITSTLSLLAYGLTNN      
Sbjct: 301 YDFIPFRPTLSVELALLRASEEEQESRRDAAAKCLSGNLITSTLSLLAYGLTNNVILNKM 360

Query: 361 -----------GITFLKYPVIGYNNRLQSSGSCLDSLNDFRITACPWDPLIKGEFFYETS 420
                      GITFLKYPVIGYNNRLQSSGSCLDSLNDFRITACPWDP IKGEFFYETS
Sbjct: 361 YNIYMSYINIVGITFLKYPVIGYNNRLQSSGSCLDSLNDFRITACPWDPSIKGEFFYETS 420

Query: 421 VSIELTVVKSFIEDVQKLAELEPMAFCGLELYNGILMRYVTASTAYLGKQQDSVEFDLTY 480
           VSIELTVVKSFIEDVQKLAELEPMAFCGLELYNGILMRYVTASTAYLGKQQDSVEFDLTY
Sbjct: 421 VSIELTVVKSFIEDVQKLAELEPMAFCGLELYNGILMRYVTASTAYLGKQQDSVEFDLTY 480

Query: 481 YRSKDPMSPRLFEDVIEEVEQMAVFKYGGIPHWGKNRNVAFHQVFHKYRNIEKFLKIMKE 540
           YRSKDPMSPRLFEDVIEEVEQMAVFKYGGIPHWGKNRNVAFHQVFHKYRNIEKFLKIMKE
Sbjct: 481 YRSKDPMSPRLFEDVIEEVEQMAVFKYGGIPHWGKNRNVAFHQVFHKYRNIEKFLKIMKE 540

Query: 541 YDPHRLFSNEWTDQILGVEGTVNIYKDGCALEGLCICSKHTHCAPSKGYFCRAGKIYKDA 596
           YDPHRLFSNEWTDQILGVEGTVNIYKDGCALEGLCICSKHTHCAPSKGYFCRAGKIYKDA
Sbjct: 541 YDPHRLFSNEWTDQILGVEGTVNIYKDGCALEGLCICSKHTHCAPSKGYFCRAGKIYKDA 600

[Close]
BLAST of CSPI03G07140 vs. ExPASy TrEMBL
Match: A0A1S3AUG3 (L-gulonolactone oxidase OS=Cucumis melo OX=3656 GN=LOC103483108 PE=3 SV=1)

HSP 1 Score: 1122.8 bits (2903), Expect = 0.0e+00
Identity = 545/596 (91.44%), Postives = 566/596 (94.97%), Query Frame = 0 

Query: 1   MRR-WSWRSYCVVSCPSSVVFMFLVLVSECSPPGDPIKCVSVSPNKYANCTITNFYGSFP 60
           MRR W W SYCV  C +S VFMFLVLVSECSPP DPIKC SVSPNKYANCTITN YG+FP
Sbjct: 1   MRRWWWWSSYCVGICSTSAVFMFLVLVSECSPPEDPIKCGSVSPNKYANCTITNSYGAFP 60

Query: 61  DRSICRVAEVIYPTTEAEVMLAVAAATRGLRKMKVGTRFSHSIPKLACPEGEEGVVISTK 120
           DRSICRVAEVIYPTTEAEVMLAVAAATRGLRKMKVGTRFSHSIPKLACPEGEEGVVISTK
Sbjct: 61  DRSICRVAEVIYPTTEAEVMLAVAAATRGLRKMKVGTRFSHSIPKLACPEGEEGVVISTK 120

Query: 121 YLNRTVKVDVEGMSISVEGGATLRQIIEDAATAGLALPSTPYWWGLTIGGLLSTGAHGSS 180
           YLNRTVKVDVEGMSISVEGGATLRQIIEDAATAGLALP TPYWWGLTIGGLLSTGAHGSS
Sbjct: 121 YLNRTVKVDVEGMSISVEGGATLRQIIEDAATAGLALPHTPYWWGLTIGGLLSTGAHGSS 180

Query: 181 LWGKGSAVHDYVVAVQMVSPGGPEDGYAKQRTILPDDQNHLNAAKVSLGVLGVITQVTLK 240
           LWGKGS+VHDYVVAV+MVSPGG EDGYAK RTILPDDQNHLNAAKVSLGVLGVITQVTLK
Sbjct: 181 LWGKGSSVHDYVVAVKMVSPGGSEDGYAKLRTILPDDQNHLNAAKVSLGVLGVITQVTLK 240

Query: 241 LQPMFKRSITYLKRDDKDLGDEVIRFGKSYEFGDMNWYPSQRKVVYRIDTRVPSNTSGNG 300
           LQPMFKRSITYLKRDDKDLGDEV+RFG  YEFGDM WYPS RK+VYRIDTRVPSNTSGNG
Sbjct: 241 LQPMFKRSITYLKRDDKDLGDEVLRFGTHYEFGDMTWYPSHRKIVYRIDTRVPSNTSGNG 300

Query: 301 LYDFIPFRPTLSVELALLRASEEEQESRRDAAAKCLSGNLITSTLSLLAYGLTNNGITFL 360
           +YDFIPFRPT S+ LAL+RASEEEQESRRDAAAKC SGNLITSTLSLLAYGLTNNGITFL
Sbjct: 301 VYDFIPFRPTPSIGLALIRASEEEQESRRDAAAKCSSGNLITSTLSLLAYGLTNNGITFL 360

Query: 361 KYPVIGYNNRLQSSGSCLDSLNDFRITACPWDPLIKGEFFYETSVSIELTVVKSFIEDVQ 420
           KYPVIGYNNRLQSSGSCLDSLND RIT CPWDP IKGEFF++T++SIELT+VKSFIEDVQ
Sbjct: 361 KYPVIGYNNRLQSSGSCLDSLNDLRITTCPWDPSIKGEFFHQTAISIELTMVKSFIEDVQ 420

Query: 421 KLAELEPMAFCGLELYNGILMRYVTASTAYLGKQQDSVEFDLTYYRSKDPMSPRLFEDVI 480
           +LAELEPMAFCGLELYNGILMRYV ASTAYLGKQQD V+FD TYYRS+DPMSPRLFEDVI
Sbjct: 421 RLAELEPMAFCGLELYNGILMRYVRASTAYLGKQQDGVDFDFTYYRSRDPMSPRLFEDVI 480

Query: 481 EEVEQMAVFKYGGIPHWGKNRNVAFHQVFHKYRNIEKFLKIMKEYDPHRLFSNEWTDQIL 540
           EEVEQMAVFKYGGIPHWGKNRNVAFHQVFHKYR+ EKFLKIMKEYDPH LFSNEWTDQIL
Sbjct: 481 EEVEQMAVFKYGGIPHWGKNRNVAFHQVFHKYRDREKFLKIMKEYDPHGLFSNEWTDQIL 540

Query: 541 GVEGTVNIYKDGCALEGLCICSKHTHCAPSKGYFCRAGKIYKDARVCSYLQSPNHT 596
           GVEGTV IYKDGCALEGLCICS+H HCAPSKGYFCRAG+IYKDARVCSYLQSPNH+
Sbjct: 541 GVEGTVIIYKDGCALEGLCICSQHNHCAPSKGYFCRAGRIYKDARVCSYLQSPNHS 596

[Close]
BLAST of CSPI03G07140 vs. ExPASy TrEMBL
Match: A0A6J1FUH8 (L-gulonolactone oxidase OS=Cucurbita moschata OX=3662 GN=LOC111448536 PE=3 SV=1)

HSP 1 Score: 914.8 bits (2363), Expect = 1.8e-262
Identity = 445/569 (78.21%), Postives = 493/569 (86.64%), Query Frame = 0 

Query: 21  MFLVLVSECSPPGDPIKCVSVSPNKYANCTITNFYGSFPDRSICRVAEVIYPTTEAEVML 80
           + LV VSE +PP DPIKC   SPN  ANCTITN Y +FPDR+ICRV EV YPTTEAEV+ 
Sbjct: 17  VLLVSVSESTPPEDPIKC--RSPN--ANCTITNAYATFPDRAICRVGEVKYPTTEAEVIS 76

Query: 81  AVAAATRGLRKMKVGTRFSHSIPKLACPEGEEGVVISTKYLNRTVKVDVEGMSISVEGGA 140
           AVA ATR +RK+KVGTRF HSIPKLACP G +GVVISTKYLNR +KVD EGM I+VEGG 
Sbjct: 77  AVAEATRAMRKIKVGTRFYHSIPKLACPHGVDGVVISTKYLNRVLKVDGEGMRITVEGGV 136

Query: 141 TLRQIIEDAATAGLALPSTPYWWGLTIGGLLSTGAHGSSLWGKGSAVHDYVVAVQMVSPG 200
           T+R+IIE AA  GLALP  PYWWGLTIGGLLSTGAHGSSLWGKGSAVHDYVV ++MVSPG
Sbjct: 137 TVREIIEVAAAGGLALPHAPYWWGLTIGGLLSTGAHGSSLWGKGSAVHDYVVGIKMVSPG 196

Query: 201 GPEDGYAKQRTILPDDQNHLNAAKVSLGVLGVITQVTLKLQPMFKRSITYLKRDDKDLGD 260
           G +DGYAK R ++ D   HLNAAKVSLGVLG+ITQVTLKLQPMFKRSITY +RDD DL D
Sbjct: 197 GAQDGYAKVRRLV-DGHQHLNAAKVSLGVLGIITQVTLKLQPMFKRSITYFERDDTDLSD 256

Query: 261 EVIRFGKSYEFGDMNWYPSQRKVVYRIDTRVPSNTSGNGLYDFIPFRPTLSVELALLRAS 320
           +VI FG+SYEF DM WYPSQRKV+YR DTRV SNTSGNGLYDFIPFRPT S  LA++RAS
Sbjct: 257 QVISFGRSYEFADMTWYPSQRKVMYRRDTRVSSNTSGNGLYDFIPFRPTPSAALAIVRAS 316

Query: 321 EEEQESRRDAAAKCLSGNLITSTLSLLAYGLTNNGITFLKYPVIGYNNRLQSSGSCLDSL 380
           EE QE+RRDAA KC S  +ITS LSL AYGLTNNGITFLKYPVIGYNNRLQSSGSCLDS+
Sbjct: 317 EEYQEARRDAAGKCRSAKIITSLLSLSAYGLTNNGITFLKYPVIGYNNRLQSSGSCLDSV 376

Query: 381 NDFRITACPWDPLIKGEFFYETSVSIELTVVKSFIEDVQKLAELEPMAFCGLELYNGILM 440
           ND RITACPW+P IKGEFF+E  +SI+L VVK FIEDV+KL ELEPMAFCGLELYNGILM
Sbjct: 377 NDLRITACPWEPSIKGEFFHEIGISIDLKVVKGFIEDVRKLGELEPMAFCGLELYNGILM 436

Query: 441 RYVTASTAYLGKQQDSVEFDLTYYRSKDPMSPRLFEDVIEEVEQMAVFKYGGIPHWGKNR 500
           RYV ASTAYLGK++D + FD TYYRSKDPMSPRLFEDV+EEVEQMA+FKYGG PHWGKNR
Sbjct: 437 RYVKASTAYLGKEEDGINFDFTYYRSKDPMSPRLFEDVLEEVEQMALFKYGGSPHWGKNR 496

Query: 501 NVAFHQVFHKYRNIEKFLKIMKEYDPHRLFSNEWTDQILGVEGTVNIYKDGCALEGLCIC 560
           N+AF +   KY+N E+FLKI  EYDP+ LFSNEWT+QILGVEGTV+I KDGCALEGLC+C
Sbjct: 497 NIAFLEAIGKYKNRERFLKIKNEYDPYGLFSNEWTNQILGVEGTVSIDKDGCALEGLCLC 556

Query: 561 SKHTHCAPSKGYFCRAGKIYKDARVCSYL 590
           SK +HCAPSKGYFCRAG+IYK+ARVCSYL
Sbjct: 557 SKDSHCAPSKGYFCRAGRIYKEARVCSYL 580

[Close]
BLAST of CSPI03G07140 vs. ExPASy TrEMBL
Match: A0A6J1IG87 (L-gulonolactone oxidase OS=Cucurbita maxima OX=3661 GN=LOC111474382 PE=3 SV=1)

HSP 1 Score: 907.5 bits (2344), Expect = 2.9e-260
Identity = 446/576 (77.43%), Postives = 498/576 (86.46%), Query Frame = 0 

Query: 17  SVVFMFLVL---VSECSPPGDPIKCVSVSPNKYANCTITNFYGSFPDRSICRVAEVIYPT 76
           + + +FLVL   VSE +PP DPIKC   SPN  ANCTITN Y +FPDR+ICRV EV YPT
Sbjct: 10  TTLILFLVLLVSVSESTPPEDPIKC--RSPN--ANCTITNAYATFPDRAICRVGEVKYPT 69

Query: 77  TEAEVMLAVAAATRGLRKMKVGTRFSHSIPKLACPEGEEGVVISTKYLNRTVKVDVEGMS 136
           TEAE++ AVAAATR +RK+KVGTRF HSIPKLACP+G +GVVISTK+LN  +KVD EGMS
Sbjct: 70  TEAEMISAVAAATRAMRKIKVGTRFYHSIPKLACPDGVDGVVISTKFLNWVLKVDGEGMS 129

Query: 137 ISVEGGATLRQIIEDAATAGLALPSTPYWWGLTIGGLLSTGAHGSSLWGKGSAVHDYVVA 196
           I+VEGG T+R+IIE AA  GLALP  PYW GLTIGGLLSTGAHGSSLWGKGSAVHDYVV 
Sbjct: 130 ITVEGGVTVREIIEVAAAVGLALPHAPYWXGLTIGGLLSTGAHGSSLWGKGSAVHDYVVG 189

Query: 197 VQMVSPGGPEDGYAKQRTILPDDQNHLNAAKVSLGVLGVITQVTLKLQPMFKRSITYLKR 256
           ++MVSPGG +DGYAK R ++  DQ HLNAAKVSLGVLG+ITQ TLKLQPMFKRSITY +R
Sbjct: 190 MKMVSPGGAQDGYAKVRRLVDGDQ-HLNAAKVSLGVLGIITQFTLKLQPMFKRSITYFER 249

Query: 257 DDKDLGDEVIRFGKSYEFGDMNWYPSQRKVVYRIDTRVPSNTSGNGLYDFIPFRPTLSVE 316
           DD DL DEVI FG+SYEF DM WYPSQRKVVYR DTRV SNTSGNGLYDFIPF PT S  
Sbjct: 250 DDTDLSDEVISFGRSYEFADMTWYPSQRKVVYRTDTRVSSNTSGNGLYDFIPFCPTPSAG 309

Query: 317 LALLRASEEEQESRRDAAAKCLSGNLITSTLSLLAYGLTNNGITFLKYPVIGYNNRLQSS 376
           LA++RAS+E QE+RRDAA KCLS  +ITS LSL AYGLTN+GITFLKYPVIGYNNRLQSS
Sbjct: 310 LAIVRASKEYQEARRDAAGKCLSAKIITSLLSLSAYGLTNDGITFLKYPVIGYNNRLQSS 369

Query: 377 GSCLDSLNDFRITACPWDPLIKGEFFYETSVSIELTVVKSFIEDVQKLAELEPMAFCGLE 436
           GSCLDS+ND RITACPW+P IKGEFF+E  +SI+L VVK FIEDV+KL ELEPMAFCGLE
Sbjct: 370 GSCLDSVNDLRITACPWEPSIKGEFFHEIGISIDLKVVKGFIEDVRKLGELEPMAFCGLE 429

Query: 437 LYNGILMRYVTASTAYLGKQQDSVEFDLTYYRSKDPMSPRLFEDVIEEVEQMAVFKYGGI 496
           LYNGILMRYV ASTAYLGK++D + FD TYYRSKDPMSPRLFEDV+EEVEQMA+FKYGGI
Sbjct: 430 LYNGILMRYVKASTAYLGKEEDGINFDFTYYRSKDPMSPRLFEDVLEEVEQMALFKYGGI 489

Query: 497 PHWGKNRNVAFHQVFHKYRNIEKFLKIMKEYDPHRLFSNEWTDQILGVEGTVNIYKDGCA 556
           PHWGKNRN+AF +   KYRN E FLKI  EYDP+ LFSNEWT+QILGVEGTV+I KDGCA
Sbjct: 490 PHWGKNRNIAFLEAIGKYRNREGFLKIKNEYDPYGLFSNEWTNQILGVEGTVSIDKDGCA 549

Query: 557 LEGLCICSKHTHCAPSKGYFCRAGKIYKDARVCSYL 590
           LEGLC+CSK +HCAPSKGYFCRAG+IYK+ARVCSY+
Sbjct: 550 LEGLCLCSKDSHCAPSKGYFCRAGRIYKEARVCSYI 580

[Close]
BLAST of CSPI03G07140 vs. ExPASy TrEMBL
Match: A0A4U5PX32 (L-gulonolactone oxidase OS=Populus alba OX=43335 GN=D5086_0000167310 PE=3 SV=1)

HSP 1 Score: 809.3 bits (2089), Expect = 1.1e-230
Identity = 377/577 (65.34%), Postives = 469/577 (81.28%), Query Frame = 0 

Query: 16  SSVVFMFLVLVSECSPPGDPIKCVSVSPNKYANCTITNFYGSFPDRSICRVAEVIYPTTE 75
           S+   +  V + +CSPP   IKC S +     +CTITN YG+FPDRSICR A V YPTTE
Sbjct: 10  STSCLLLAVFMVDCSPPESHIKCSSTN----TDCTITNSYGTFPDRSICRAANVAYPTTE 69

Query: 76  AEVMLAVAAATRGLRKMKVGTRFSHSIPKLACPEGEEGVVISTKYLNRTVKVDVEGMSIS 135
            E++  VA AT   RK+KV TR+SHSIPKLACP+G+ G++IST YLNRT+++DV+ M+++
Sbjct: 70  EELISIVAGATVAERKVKVATRYSHSIPKLACPDGQNGLLISTDYLNRTLEIDVQSMTMT 129

Query: 136 VEGGATLRQIIEDAATAGLALPSTPYWWGLTIGGLLSTGAHGSSLWGKGSAVHDYVVAVQ 195
           VE G TLRQ+I +AA AGLALP +PYWWGLTIGGLLSTGAHGS+LWGKGSA+HDYVVA+ 
Sbjct: 130 VESGVTLRQLINEAAEAGLALPYSPYWWGLTIGGLLSTGAHGSTLWGKGSALHDYVVALT 189

Query: 196 MVSPGGPEDGYAKQRTILPDDQNHLNAAKVSLGVLGVITQVTLKLQPMFKRSITYLKRDD 255
           ++SPGGPEDGYAK R++   +   LNAAKVSLGVLGVI++VTL+LQP+FKRSI+Y+ ++D
Sbjct: 190 IISPGGPEDGYAKVRSLDESNSTELNAAKVSLGVLGVISKVTLQLQPLFKRSISYVVKED 249

Query: 256 KDLGDEVIRFGKSYEFGDMNWYPSQRKVVYRIDTRVPSNTSGNGLYDFIPFRPTLSVELA 315
            DLGD+V  FG+ +EF D+ WYPSQ K VYRID R+ SNTSGNGLYD+IPFR TLS+ LA
Sbjct: 250 ADLGDQVASFGRQHEFADITWYPSQGKAVYRIDDRISSNTSGNGLYDYIPFRSTLSLGLA 309

Query: 316 LLRASEEEQESRRDAAAKCLSGNLITSTLSLLAYGLTNNGITFLKYPVIGYNNRLQSSGS 375
            +RA+E+ QES +D   KC S  LITSTL  LAYGLTNNGI F  YP+IGY+NRLQSSG+
Sbjct: 310 AVRATEDAQESLKDPDGKCASAKLITSTLKNLAYGLTNNGILFTGYPIIGYHNRLQSSGT 369

Query: 376 CLDSLNDFRITACPWDPLIKGEFFYETSVSIELTVVKSFIEDVQKLAELEPMAFCGLELY 435
           CLDS  D  ITACPWD  IKGE+F++ + SI L+VVKSFI+DVQ L +LEP A CGLE Y
Sbjct: 370 CLDSPEDALITACPWDSRIKGEYFFQATFSISLSVVKSFIQDVQNLVKLEPRALCGLEQY 429

Query: 436 NGILMRYVTASTAYLGKQQDSVEFDLTYYRSKDPMSPRLFEDVIEEVEQMAVFKYGGIPH 495
           NGILMRYV AS+AYLGK+ D+++FD+T+YR+KDP  PRL+ED++EE+EQ+AVFKYGG+PH
Sbjct: 430 NGILMRYVKASSAYLGKEDDALDFDITFYRNKDPAKPRLYEDILEEIEQLAVFKYGGLPH 489

Query: 496 WGKNRNVAFHQVFHKYRNIEKFLKIMKEYDPHRLFSNEWTDQILGVEGTVNIYKDGCALE 555
           WGKNRN+ F+    KY+N   FL++ + YDP  LFSNEWTDQ+LG++G VNI KDGCALE
Sbjct: 490 WGKNRNLVFNGALKKYKNAGAFLRVKEMYDPLGLFSNEWTDQVLGLKGDVNIIKDGCALE 549

Query: 556 GLCICSKHTHCAPSKGYFCRAGKIYKDARVCSYLQSP 593
           GLCICS+ THCAPSKGY CR GKIY++ARVC+++ +P
Sbjct: 550 GLCICSQDTHCAPSKGYLCRPGKIYQEARVCAHVSTP 582

[Close]
BLAST of CSPI03G07140 vs. NCBI nr
Match: XP_004134280.1 (probable L-gulonolactone oxidase 6 [Cucumis sativus] >KAE8650211.1 hypothetical protein Csa_009956 [Cucumis sativus])

HSP 1 Score: 1216.8 bits (3147), Expect = 0.0e+00
Identity = 593/595 (99.66%), Postives = 594/595 (99.83%), Query Frame = 0 

Query: 1   MRRWSWRSYCVVSCPSSVVFMFLVLVSECSPPGDPIKCVSVSPNKYANCTITNFYGSFPD 60
           MRRWSWRSYCVVSCPSSVVFMFLVLVSECSPPGDPIKCVSVSPNKYANCTITNFYGSFPD
Sbjct: 1   MRRWSWRSYCVVSCPSSVVFMFLVLVSECSPPGDPIKCVSVSPNKYANCTITNFYGSFPD 60

Query: 61  RSICRVAEVIYPTTEAEVMLAVAAATRGLRKMKVGTRFSHSIPKLACPEGEEGVVISTKY 120
           RSICRVAEVIYPTTEAEVMLAVAAATRGLRKMKVGTRFSHSIPKLACPEGEEGVVISTKY
Sbjct: 61  RSICRVAEVIYPTTEAEVMLAVAAATRGLRKMKVGTRFSHSIPKLACPEGEEGVVISTKY 120

Query: 121 LNRTVKVDVEGMSISVEGGATLRQIIEDAATAGLALPSTPYWWGLTIGGLLSTGAHGSSL 180
           LNRTVKVDVEGMSISVEGGATLRQIIEDAATAGLALPSTPYWWGLTIGGLLSTGAHGSSL
Sbjct: 121 LNRTVKVDVEGMSISVEGGATLRQIIEDAATAGLALPSTPYWWGLTIGGLLSTGAHGSSL 180

Query: 181 WGKGSAVHDYVVAVQMVSPGGPEDGYAKQRTILPDDQNHLNAAKVSLGVLGVITQVTLKL 240
           WGKGSAVHDYVVAVQMVSPGGPEDGYAKQRTILPDDQNHLNAAKVSLGVLGVITQVTLKL
Sbjct: 181 WGKGSAVHDYVVAVQMVSPGGPEDGYAKQRTILPDDQNHLNAAKVSLGVLGVITQVTLKL 240

Query: 241 QPMFKRSITYLKRDDKDLGDEVIRFGKSYEFGDMNWYPSQRKVVYRIDTRVPSNTSGNGL 300
           QPMFKRSITYLKRDDKDLGDEVIRFGKSYEFGDM+WYPSQRKVVYRIDTRVPSNTSGNGL
Sbjct: 241 QPMFKRSITYLKRDDKDLGDEVIRFGKSYEFGDMSWYPSQRKVVYRIDTRVPSNTSGNGL 300

Query: 301 YDFIPFRPTLSVELALLRASEEEQESRRDAAAKCLSGNLITSTLSLLAYGLTNNGITFLK 360
           YDFIPFRPTLSVELALLRASEEEQESRRDAAAKCLSGNLITSTLSLLAYGLTNNGITFLK
Sbjct: 301 YDFIPFRPTLSVELALLRASEEEQESRRDAAAKCLSGNLITSTLSLLAYGLTNNGITFLK 360

Query: 361 YPVIGYNNRLQSSGSCLDSLNDFRITACPWDPLIKGEFFYETSVSIELTVVKSFIEDVQK 420
           YPVIGYNNRLQSSGSCLDSLNDFRITACPWDP IKGEFFYETSVSIELTVVKSFIEDVQK
Sbjct: 361 YPVIGYNNRLQSSGSCLDSLNDFRITACPWDPSIKGEFFYETSVSIELTVVKSFIEDVQK 420

Query: 421 LAELEPMAFCGLELYNGILMRYVTASTAYLGKQQDSVEFDLTYYRSKDPMSPRLFEDVIE 480
           LAELEPMAFCGLELYNGILMRYVTASTAYLGKQQDSVEFDLTYYRSKDPMSPRLFEDVIE
Sbjct: 421 LAELEPMAFCGLELYNGILMRYVTASTAYLGKQQDSVEFDLTYYRSKDPMSPRLFEDVIE 480

Query: 481 EVEQMAVFKYGGIPHWGKNRNVAFHQVFHKYRNIEKFLKIMKEYDPHRLFSNEWTDQILG 540
           EVEQMAVFKYGGIPHWGKNRNVAFHQVFHKYRNIEKFLKIMKEYDPHRLFSNEWTDQILG
Sbjct: 481 EVEQMAVFKYGGIPHWGKNRNVAFHQVFHKYRNIEKFLKIMKEYDPHRLFSNEWTDQILG 540

Query: 541 VEGTVNIYKDGCALEGLCICSKHTHCAPSKGYFCRAGKIYKDARVCSYLQSPNHT 596
           VEGTVNIYKDGCALEGLCICSKHTHCAPSKGYFCRAGKIYKDARVCSYLQSPNHT
Sbjct: 541 VEGTVNIYKDGCALEGLCICSKHTHCAPSKGYFCRAGKIYKDARVCSYLQSPNHT 595

[Close]
BLAST of CSPI03G07140 vs. NCBI nr
Match: XP_008437774.1 (PREDICTED: probable L-gulonolactone oxidase 6 [Cucumis melo])

HSP 1 Score: 1122.8 bits (2903), Expect = 0.0e+00
Identity = 545/596 (91.44%), Postives = 566/596 (94.97%), Query Frame = 0 

Query: 1   MRR-WSWRSYCVVSCPSSVVFMFLVLVSECSPPGDPIKCVSVSPNKYANCTITNFYGSFP 60
           MRR W W SYCV  C +S VFMFLVLVSECSPP DPIKC SVSPNKYANCTITN YG+FP
Sbjct: 1   MRRWWWWSSYCVGICSTSAVFMFLVLVSECSPPEDPIKCGSVSPNKYANCTITNSYGAFP 60

Query: 61  DRSICRVAEVIYPTTEAEVMLAVAAATRGLRKMKVGTRFSHSIPKLACPEGEEGVVISTK 120
           DRSICRVAEVIYPTTEAEVMLAVAAATRGLRKMKVGTRFSHSIPKLACPEGEEGVVISTK
Sbjct: 61  DRSICRVAEVIYPTTEAEVMLAVAAATRGLRKMKVGTRFSHSIPKLACPEGEEGVVISTK 120

Query: 121 YLNRTVKVDVEGMSISVEGGATLRQIIEDAATAGLALPSTPYWWGLTIGGLLSTGAHGSS 180
           YLNRTVKVDVEGMSISVEGGATLRQIIEDAATAGLALP TPYWWGLTIGGLLSTGAHGSS
Sbjct: 121 YLNRTVKVDVEGMSISVEGGATLRQIIEDAATAGLALPHTPYWWGLTIGGLLSTGAHGSS 180

Query: 181 LWGKGSAVHDYVVAVQMVSPGGPEDGYAKQRTILPDDQNHLNAAKVSLGVLGVITQVTLK 240
           LWGKGS+VHDYVVAV+MVSPGG EDGYAK RTILPDDQNHLNAAKVSLGVLGVITQVTLK
Sbjct: 181 LWGKGSSVHDYVVAVKMVSPGGSEDGYAKLRTILPDDQNHLNAAKVSLGVLGVITQVTLK 240

Query: 241 LQPMFKRSITYLKRDDKDLGDEVIRFGKSYEFGDMNWYPSQRKVVYRIDTRVPSNTSGNG 300
           LQPMFKRSITYLKRDDKDLGDEV+RFG  YEFGDM WYPS RK+VYRIDTRVPSNTSGNG
Sbjct: 241 LQPMFKRSITYLKRDDKDLGDEVLRFGTHYEFGDMTWYPSHRKIVYRIDTRVPSNTSGNG 300

Query: 301 LYDFIPFRPTLSVELALLRASEEEQESRRDAAAKCLSGNLITSTLSLLAYGLTNNGITFL 360
           +YDFIPFRPT S+ LAL+RASEEEQESRRDAAAKC SGNLITSTLSLLAYGLTNNGITFL
Sbjct: 301 VYDFIPFRPTPSIGLALIRASEEEQESRRDAAAKCSSGNLITSTLSLLAYGLTNNGITFL 360

Query: 361 KYPVIGYNNRLQSSGSCLDSLNDFRITACPWDPLIKGEFFYETSVSIELTVVKSFIEDVQ 420
           KYPVIGYNNRLQSSGSCLDSLND RIT CPWDP IKGEFF++T++SIELT+VKSFIEDVQ
Sbjct: 361 KYPVIGYNNRLQSSGSCLDSLNDLRITTCPWDPSIKGEFFHQTAISIELTMVKSFIEDVQ 420

Query: 421 KLAELEPMAFCGLELYNGILMRYVTASTAYLGKQQDSVEFDLTYYRSKDPMSPRLFEDVI 480
           +LAELEPMAFCGLELYNGILMRYV ASTAYLGKQQD V+FD TYYRS+DPMSPRLFEDVI
Sbjct: 421 RLAELEPMAFCGLELYNGILMRYVRASTAYLGKQQDGVDFDFTYYRSRDPMSPRLFEDVI 480

Query: 481 EEVEQMAVFKYGGIPHWGKNRNVAFHQVFHKYRNIEKFLKIMKEYDPHRLFSNEWTDQIL 540
           EEVEQMAVFKYGGIPHWGKNRNVAFHQVFHKYR+ EKFLKIMKEYDPH LFSNEWTDQIL
Sbjct: 481 EEVEQMAVFKYGGIPHWGKNRNVAFHQVFHKYRDREKFLKIMKEYDPHGLFSNEWTDQIL 540

Query: 541 GVEGTVNIYKDGCALEGLCICSKHTHCAPSKGYFCRAGKIYKDARVCSYLQSPNHT 596
           GVEGTV IYKDGCALEGLCICS+H HCAPSKGYFCRAG+IYKDARVCSYLQSPNH+
Sbjct: 541 GVEGTVIIYKDGCALEGLCICSQHNHCAPSKGYFCRAGRIYKDARVCSYLQSPNHS 596

[Close]
BLAST of CSPI03G07140 vs. NCBI nr
Match: XP_038882597.1 (LOW QUALITY PROTEIN: probable L-gulonolactone oxidase 6 [Benincasa hispida])

HSP 1 Score: 1030.0 bits (2662), Expect = 8.0e-297
Identity = 494/591 (83.59%), Postives = 544/591 (92.05%), Query Frame = 0 

Query: 1   MRRWSWRSYCVVSCPSSVVFMFLVLVSECSPPGDPIKCVSVSPNKYANCTITNFYGSFPD 60
           MRR  W SY V     ++VF+ +V++S+CSPP DPIKC SVSPNK+ANCTITN Y +FPD
Sbjct: 1   MRRCWWWSYNV-----ALVFLLVVILSDCSPPEDPIKCGSVSPNKHANCTITNAYAAFPD 60

Query: 61  RSICRVAEVIYPTTEAEVMLAVAAATRGLRKMKVGTRFSHSIPKLACPEGEEGVVISTKY 120
           R+ICR AEVIYPTTEAEVMLAVAAATRG+RKMKVGTRFSHSIPKL+CPEGE+GVVISTKY
Sbjct: 61  RTICRAAEVIYPTTEAEVMLAVAAATRGMRKMKVGTRFSHSIPKLSCPEGEDGVVISTKY 120

Query: 121 LNRTVKVDVEGMSISVEGGATLRQIIEDAATAGLALPSTPYWWGLTIGGLLSTGAHGSSL 180
           LNRT+ VDVEGMSISVEGGAT+RQIIE+ A AGLALP TPYWWGLTIGGLLSTGAHGSSL
Sbjct: 121 LNRTLMVDVEGMSISVEGGATVRQIIEEVAAAGLALPHTPYWWGLTIGGLLSTGAHGSSL 180

Query: 181 WGKGSAVHDYVVAVQMVSPGGPEDGYAKQRTILPDDQNHLNAAKVSLGVLGVITQVTLKL 240
           WGKGSAVHDYVVA+QMV+PGGP+DGYAK RT+LP DQ HLNAAKVSLGVLGVITQVTLKL
Sbjct: 181 WGKGSAVHDYVVAIQMVTPGGPQDGYAKLRTLLPADQYHLNAAKVSLGVLGVITQVTLKL 240

Query: 241 QPMFKRSITYLKRDDKDLGDEVIRFGKSYEFGDMNWYPSQRKVVYRIDTRVPSNTSGNGL 300
           +PMFKRSITYLKRDDKDLGDEV+ FG+SYEFGDM WYPSQRKVVYRID+RVPSNTSGNG 
Sbjct: 241 EPMFKRSITYLKRDDKDLGDEVVSFGRSYEFGDMIWYPSQRKVVYRIDSRVPSNTSGNGF 300

Query: 301 YDFIPFRPTLSVELALLRASEEEQESRRDAAAKCLSGNLITSTLSLLAYGLTNNGITFLK 360
           YDFIPFRPT S+ LAL+RASEE+QESRRDA  KC+S NL TSTLS LAYGLTNNGI+FLK
Sbjct: 301 YDFIPFRPTPSLALALVRASEEKQESRRDAVGKCISANLFTSTLSQLAYGLTNNGISFLK 360

Query: 361 YPVIGYNNRLQSSGSCLDSLNDFRITACPWDPLIKGEFFYETSVSIELTVVKSFIEDVQK 420
           YPVIG +NRLQSSGSCL SL+DFRITACPWDPLIKGEFF++T++SI+L VVKSF++DVQK
Sbjct: 361 YPVIGDSNRLQSSGSCLHSLDDFRITACPWDPLIKGEFFHQTAISIDLGVVKSFVQDVQK 420

Query: 421 LAELEPMAFCGLELYNGILMRYVTASTAYLGKQQDSVEFDLTYYRSKDPMSPRLFEDVIE 480
           LAELEPMAFCGLELYNGILMRYVTASTAYLGKQQ  V+FD TYYRS+DPMSPRLFEDV E
Sbjct: 421 LAELEPMAFCGLELYNGILMRYVTASTAYLGKQQHGVDFDFTYYRSRDPMSPRLFEDVFE 480

Query: 481 EVEQMAVFKYGGIPHWGKNRNVAFHQVFHKYRNIEKFLKIMKEYDPHRLFSNEWTDQILG 540
           EVEQMAVFKYGGIPHWGKNRNVAFHQ F KYRN + F+KIMKE+DPH LFSNEWTDQILG
Sbjct: 481 EVEQMAVFKYGGIPHWGKNRNVAFHQAFRKYRNRDSFVKIMKEFDPHGLFSNEWTDQILG 540

Query: 541 VEGTVNIYKDGCALEGLCICSKHTHCAPSKGYFCRAGKIYKDARVCSYLQS 592
           ++GTVNIYKDGCALEGLCICS+HTHCAP++GYFC  G+IYK+ARVCSYL S
Sbjct: 541 LKGTVNIYKDGCALEGLCICSQHTHCAPTEGYFCTPGRIYKEARVCSYLGS 586

[Close]
BLAST of CSPI03G07140 vs. NCBI nr
Match: XP_023540226.1 (probable L-gulonolactone oxidase 6 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 921.0 bits (2379), Expect = 5.2e-264
Identity = 450/569 (79.09%), Postives = 496/569 (87.17%), Query Frame = 0 

Query: 21  MFLVLVSECSPPGDPIKCVSVSPNKYANCTITNFYGSFPDRSICRVAEVIYPTTEAEVML 80
           + LV VSE +PP DPIKC   SPN  ANCTITN Y +FPDR+ICRV EV YPTTEAEV+ 
Sbjct: 17  VLLVSVSESTPPEDPIKC--RSPN--ANCTITNAYATFPDRAICRVGEVKYPTTEAEVIS 76

Query: 81  AVAAATRGLRKMKVGTRFSHSIPKLACPEGEEGVVISTKYLNRTVKVDVEGMSISVEGGA 140
           AVA ATR +RK+KVGTRF HSIPKLACP+G +GVVISTKYLNR +KVD EGMSI+VEGG 
Sbjct: 77  AVAEATRAMRKIKVGTRFYHSIPKLACPDGVDGVVISTKYLNRVLKVDGEGMSITVEGGV 136

Query: 141 TLRQIIEDAATAGLALPSTPYWWGLTIGGLLSTGAHGSSLWGKGSAVHDYVVAVQMVSPG 200
           T+R+IIE AA  GLALP  PYWWGLTIGGLLSTGAHGSSLWGKGSAVHDYVV ++MVSPG
Sbjct: 137 TVREIIEVAAAGGLALPHAPYWWGLTIGGLLSTGAHGSSLWGKGSAVHDYVVGIKMVSPG 196

Query: 201 GPEDGYAKQRTILPDDQNHLNAAKVSLGVLGVITQVTLKLQPMFKRSITYLKRDDKDLGD 260
           G +DGYAK R ++  DQ HLNAAKVSLGVLG+ITQVTLKLQPMFKRSITY +RDD DL D
Sbjct: 197 GAQDGYAKVRRLVDGDQ-HLNAAKVSLGVLGIITQVTLKLQPMFKRSITYFERDDTDLSD 256

Query: 261 EVIRFGKSYEFGDMNWYPSQRKVVYRIDTRVPSNTSGNGLYDFIPFRPTLSVELALLRAS 320
           +VI FG+SYEF DM WYPSQRKVVYR DTRV SNTSGNGLYDFIPFRPT S  LA++RAS
Sbjct: 257 QVISFGRSYEFADMTWYPSQRKVVYRRDTRVSSNTSGNGLYDFIPFRPTPSPALAIVRAS 316

Query: 321 EEEQESRRDAAAKCLSGNLITSTLSLLAYGLTNNGITFLKYPVIGYNNRLQSSGSCLDSL 380
           EE QE+RRDAA KCLS  +ITS LSL AYGLTNNGITFLKYPVIGYNNRLQSSGSCLDS+
Sbjct: 317 EEYQEARRDAAGKCLSAKIITSLLSLSAYGLTNNGITFLKYPVIGYNNRLQSSGSCLDSV 376

Query: 381 NDFRITACPWDPLIKGEFFYETSVSIELTVVKSFIEDVQKLAELEPMAFCGLELYNGILM 440
           ND RITACPWDP IKGEFF+E  +SI+L VVK FIEDV+KL ELEPMAFCGLELYNGILM
Sbjct: 377 NDLRITACPWDPSIKGEFFHEIGISIDLKVVKGFIEDVRKLGELEPMAFCGLELYNGILM 436

Query: 441 RYVTASTAYLGKQQDSVEFDLTYYRSKDPMSPRLFEDVIEEVEQMAVFKYGGIPHWGKNR 500
           RYV ASTAYLGK++D + FD TYYRSKDPMSPRLFEDV+EEVEQMA+FKYGG PHWGKNR
Sbjct: 437 RYVKASTAYLGKEEDGINFDFTYYRSKDPMSPRLFEDVLEEVEQMALFKYGGSPHWGKNR 496

Query: 501 NVAFHQVFHKYRNIEKFLKIMKEYDPHRLFSNEWTDQILGVEGTVNIYKDGCALEGLCIC 560
           N+AF +   KY+N E FLKI  EYDP+ LFSNEWT+QILGVEGTV+I KDGCALEGLC+C
Sbjct: 497 NIAFLKAVGKYKNREGFLKIKNEYDPYGLFSNEWTNQILGVEGTVSIDKDGCALEGLCLC 556

Query: 561 SKHTHCAPSKGYFCRAGKIYKDARVCSYL 590
           SK +HCAPSKGYFCRAG+IYK+ARVCSYL
Sbjct: 557 SKDSHCAPSKGYFCRAGRIYKEARVCSYL 580

[Close]
BLAST of CSPI03G07140 vs. NCBI nr
Match: XP_022943972.1 (probable L-gulonolactone oxidase 6 [Cucurbita moschata])

HSP 1 Score: 914.8 bits (2363), Expect = 3.8e-262
Identity = 445/569 (78.21%), Postives = 493/569 (86.64%), Query Frame = 0 

Query: 21  MFLVLVSECSPPGDPIKCVSVSPNKYANCTITNFYGSFPDRSICRVAEVIYPTTEAEVML 80
           + LV VSE +PP DPIKC   SPN  ANCTITN Y +FPDR+ICRV EV YPTTEAEV+ 
Sbjct: 17  VLLVSVSESTPPEDPIKC--RSPN--ANCTITNAYATFPDRAICRVGEVKYPTTEAEVIS 76

Query: 81  AVAAATRGLRKMKVGTRFSHSIPKLACPEGEEGVVISTKYLNRTVKVDVEGMSISVEGGA 140
           AVA ATR +RK+KVGTRF HSIPKLACP G +GVVISTKYLNR +KVD EGM I+VEGG 
Sbjct: 77  AVAEATRAMRKIKVGTRFYHSIPKLACPHGVDGVVISTKYLNRVLKVDGEGMRITVEGGV 136

Query: 141 TLRQIIEDAATAGLALPSTPYWWGLTIGGLLSTGAHGSSLWGKGSAVHDYVVAVQMVSPG 200
           T+R+IIE AA  GLALP  PYWWGLTIGGLLSTGAHGSSLWGKGSAVHDYVV ++MVSPG
Sbjct: 137 TVREIIEVAAAGGLALPHAPYWWGLTIGGLLSTGAHGSSLWGKGSAVHDYVVGIKMVSPG 196

Query: 201 GPEDGYAKQRTILPDDQNHLNAAKVSLGVLGVITQVTLKLQPMFKRSITYLKRDDKDLGD 260
           G +DGYAK R ++ D   HLNAAKVSLGVLG+ITQVTLKLQPMFKRSITY +RDD DL D
Sbjct: 197 GAQDGYAKVRRLV-DGHQHLNAAKVSLGVLGIITQVTLKLQPMFKRSITYFERDDTDLSD 256

Query: 261 EVIRFGKSYEFGDMNWYPSQRKVVYRIDTRVPSNTSGNGLYDFIPFRPTLSVELALLRAS 320
           +VI FG+SYEF DM WYPSQRKV+YR DTRV SNTSGNGLYDFIPFRPT S  LA++RAS
Sbjct: 257 QVISFGRSYEFADMTWYPSQRKVMYRRDTRVSSNTSGNGLYDFIPFRPTPSAALAIVRAS 316

Query: 321 EEEQESRRDAAAKCLSGNLITSTLSLLAYGLTNNGITFLKYPVIGYNNRLQSSGSCLDSL 380
           EE QE+RRDAA KC S  +ITS LSL AYGLTNNGITFLKYPVIGYNNRLQSSGSCLDS+
Sbjct: 317 EEYQEARRDAAGKCRSAKIITSLLSLSAYGLTNNGITFLKYPVIGYNNRLQSSGSCLDSV 376

Query: 381 NDFRITACPWDPLIKGEFFYETSVSIELTVVKSFIEDVQKLAELEPMAFCGLELYNGILM 440
           ND RITACPW+P IKGEFF+E  +SI+L VVK FIEDV+KL ELEPMAFCGLELYNGILM
Sbjct: 377 NDLRITACPWEPSIKGEFFHEIGISIDLKVVKGFIEDVRKLGELEPMAFCGLELYNGILM 436

Query: 441 RYVTASTAYLGKQQDSVEFDLTYYRSKDPMSPRLFEDVIEEVEQMAVFKYGGIPHWGKNR 500
           RYV ASTAYLGK++D + FD TYYRSKDPMSPRLFEDV+EEVEQMA+FKYGG PHWGKNR
Sbjct: 437 RYVKASTAYLGKEEDGINFDFTYYRSKDPMSPRLFEDVLEEVEQMALFKYGGSPHWGKNR 496

Query: 501 NVAFHQVFHKYRNIEKFLKIMKEYDPHRLFSNEWTDQILGVEGTVNIYKDGCALEGLCIC 560
           N+AF +   KY+N E+FLKI  EYDP+ LFSNEWT+QILGVEGTV+I KDGCALEGLC+C
Sbjct: 497 NIAFLEAIGKYKNRERFLKIKNEYDPYGLFSNEWTNQILGVEGTVSIDKDGCALEGLCLC 556

Query: 561 SKHTHCAPSKGYFCRAGKIYKDARVCSYL 590
           SK +HCAPSKGYFCRAG+IYK+ARVCSYL
Sbjct: 557 SKDSHCAPSKGYFCRAGRIYKEARVCSYL 580

[Close]
BLAST of CSPI03G07140 vs. TAIR 10
Match: AT2G46760.1 (D-arabinono-1,4-lactone oxidase family protein )

HSP 1 Score: 759.6 bits (1960), Expect = 1.9e-219
Identity = 362/583 (62.09%), Postives = 447/583 (76.67%), Query Frame = 0 

Query: 6   WRSYCVVSCPSSVVFMFLVLVSECSPPGDPIKCVSVSPNKYANCTITNFYGSFPDRSICR 65
           WR+  VV C S++VF  +      +PP DP+KCVS +     NCT+TN YG+FPDRS CR
Sbjct: 17  WRTIFVVHCISTLVFTTI-----STPPEDPVKCVSGN----TNCTVTNSYGAFPDRSTCR 76

Query: 66  VAEVIYPTTEAEVMLAVAAATRGLRKMKVGTRFSHSIPKLACPEGEEGVVISTKYLNRTV 125
            A V YPTTEAE++  VAAAT+  RKM+V TR+SHSI KLAC +G +G++ISTK+LN TV
Sbjct: 77  AANVAYPTTEAELISVVAAATKAGRKMRVTTRYSHSITKLACTDGTDGLLISTKFLNHTV 136

Query: 126 KVDVEGMSISVEGGATLRQIIEDAATAGLALPSTPYWWGLTIGGLLSTGAHGSSLWGKGS 185
           + D   M+++VE G TLRQ+I +AA  GLALP  PYWWGLT+GG++ TGAHGSSLWGKGS
Sbjct: 137 RTDATAMTLTVESGVTLRQLIAEAAKVGLALPYAPYWWGLTVGGMMGTGAHGSSLWGKGS 196

Query: 186 AVHDYVVAVQMVSPGGPEDGYAKQRTIL-PDDQNHLNAAKVSLGVLGVITQVTLKLQPMF 245
           AVHDYV  +++VSPG   DG+AK R +         NAAKVSLGVLGVI+QVTLKLQPMF
Sbjct: 197 AVHDYVTEIRIVSPGSVNDGFAKVRVLRETTTPKEFNAAKVSLGVLGVISQVTLKLQPMF 256

Query: 246 KRSITYLKRDDKDLGDEVIRFGKSYEFGDMNWYPSQRKVVYRIDTRVPSNTSGNGLYDFI 305
           KRS+ Y+ R+D D GD+ + FG  +EF D  W PSQ KVVYR+D RV  NTSGNGL DF+
Sbjct: 257 KRSLRYVMRNDSDFGDQAVTFGMKHEFADFIWLPSQGKVVYRMDDRVAVNTSGNGLLDFM 316

Query: 306 PFRPTLSVELALLRASEEEQESRRDAAAKCLSGNLITSTLSLLAYGLTNNGITFLKYPVI 365
           PFR  LS  LA++R+SEE QE  RDA  KC    LITSTL   +YGLTNNG+ F  YPVI
Sbjct: 317 PFRSQLSAALAIIRSSEETQERFRDANGKCAGATLITSTLFATSYGLTNNGMIFTGYPVI 376

Query: 366 GYNNRLQSSGSCLDSLNDFRITACPWDPLIKGEFFYETSVSIELTVVKSFIEDVQKLAEL 425
           G  NR+ SSGSCLDSL+D  ITACPWD  IK EFF++T+ SI LT VKSFI D++ L ++
Sbjct: 377 GSQNRMMSSGSCLDSLHDGLITACPWDSRIKSEFFHQTTFSIPLTQVKSFINDIKSLVKI 436

Query: 426 EPMAFCGLELYNGILMRYVTASTAYLGKQQDSVEFDLTYYRSKDPMSPRLFEDVIEEVEQ 485
           E  + C LELY+GILMRYVT+S AYLGK+ ++++FDLTYYR+KDP+SPRL+ED IEE+EQ
Sbjct: 437 ESKSLCVLELYDGILMRYVTSSPAYLGKETEALDFDLTYYRAKDPLSPRLYEDFIEEIEQ 496

Query: 486 MAVFKYGGIPHWGKNRNVAFHQVFHKYRNIEKFLKIMKEYDPHRLFSNEWTDQILGVEGT 545
           +A+FKY  +PHWGKNRN+AF  V  KY+N+  FLK+ + YDP  LFS+EWTDQILG++G 
Sbjct: 497 IALFKYNALPHWGKNRNLAFDGVIKKYKNVPAFLKVKESYDPMGLFSSEWTDQILGIKGN 556

Query: 546 VNIYKDGCALEGLCICSKHTHCAPSKGYFCRAGKIYKDARVCS 588
           V I KDGCALEGLC+CS+  HCAP+KGYFCR GK+YK+ARVC+
Sbjct: 557 VTIIKDGCALEGLCVCSEDAHCAPTKGYFCRPGKVYKEARVCT 590

[Close]
BLAST of CSPI03G07140 vs. TAIR 10
Match: AT2G46750.1 (D-arabinono-1,4-lactone oxidase family protein )

HSP 1 Score: 737.3 bits (1902), Expect = 1.0e-212
Identity = 347/559 (62.08%), Postives = 428/559 (76.57%), Query Frame = 0 

Query: 30  SPPGDPIKCVSVSPNKYANCTITNFYGSFPDRSICRVAEVIYPTTEAEVMLAVAAATRGL 89
           +PP DP+KCVS +     NCT+TN YG+FPDRS CR A V YP TEAE++  VAAAT+  
Sbjct: 28  TPPDDPVKCVSGN----TNCTVTNSYGAFPDRSTCRAANVAYPKTEAELVSVVAAATQAG 87

Query: 90  RKMKVGTRFSHSIPKLACPEGEEGVVISTKYLNRTVKVDVEGMSISVEGGATLRQIIEDA 149
           RKM+V TR+SHSI KL C +G EG+ ISTK+LN TV+ D   M+++VE G TLRQ+I +A
Sbjct: 88  RKMRVTTRYSHSITKLVCTDGTEGLFISTKFLNHTVQADATAMTMTVESGMTLRQLIVEA 147

Query: 150 ATAGLALPSTPYWWGLTIGGLLSTGAHGSSLWGKGSAVHDYVVAVQMVSPGGPEDGYAKQ 209
           A  GLALP  PYWWGLT+GG++ TGAHGSSLWGKGSAVHDYV  ++MVSPG   +G+AK 
Sbjct: 148 AKVGLALPYAPYWWGLTVGGMMGTGAHGSSLWGKGSAVHDYVTEIRMVSPGSVNEGFAKI 207

Query: 210 RTIL-PDDQNHLNAAKVSLGVLGVITQVTLKLQPMFKRSITYLKRDDKDLGDEVIRFGKS 269
           R +      N  NAAKVSLGVLGVI+QVT +LQPMFKRS+TY  R+D D  D+ + FGK 
Sbjct: 208 RILSETTTPNEFNAAKVSLGVLGVISQVTFELQPMFKRSLTYTMRNDSDFEDQAVTFGKK 267

Query: 270 YEFGDMNWYPSQRKVVYRIDTRVPSNTSGNGLYDFIPFRPTLSVELALLRASEEEQESRR 329
           +EF D  W PSQ KVVYR D RV  NTSGNGL+DF+PFR  LS  +A++R SEE QE  R
Sbjct: 268 HEFADFIWLPSQGKVVYRRDDRVAVNTSGNGLFDFLPFRSQLSAAIAIIRTSEETQERFR 327

Query: 330 DAAAKCLSGNLITSTLSLLAYGLTNNGITFLKYPVIGYNNRLQSSGSCLDSLNDFRITAC 389
           DA  KC+   +I+STL   +YGLTNNGI F  YPV+G  NR+ SSGSCLDSL D  ITAC
Sbjct: 328 DANGKCVGATIISSTLFAPSYGLTNNGIIFTGYPVVGSQNRMMSSGSCLDSLQDGLITAC 387

Query: 390 PWDPLIKGEFFYETSVSIELTVVKSFIEDVQKLAELEPMAFCGLELYNGILMRYVTASTA 449
            WD  IKGEFF++T++S+ LT VKSFI D++ L ++E  + CGLEL+ GILMRYVT+S A
Sbjct: 388 AWDSRIKGEFFHQTTLSVPLTQVKSFISDIKSLVKIEQKSLCGLELHYGILMRYVTSSPA 447

Query: 450 YLGKQQDSVEFDLTYYRSKDPMSPRLFEDVIEEVEQMAVFKYGGIPHWGKNRNVAFHQVF 509
           YLGK+ ++++FD+TYYR+KDP++PRL+ED IEE+EQ+A+FKY  +PHWGKNRN+AF  V 
Sbjct: 448 YLGKETEALDFDITYYRAKDPLTPRLYEDFIEEIEQIALFKYNALPHWGKNRNLAFDGVI 507

Query: 510 HKYRNIEKFLKIMKEYDPHRLFSNEWTDQILGVEGTVNIYKDGCALEGLCICSKHTHCAP 569
            KY N   FLK+   YDP  LFS+EWTDQILG++G  +I KDGCALEGLCICSK  HCAP
Sbjct: 508 RKYNNAPAFLKVKDSYDPKGLFSSEWTDQILGIKGNASIVKDGCALEGLCICSKDAHCAP 567

Query: 570 SKGYFCRAGKIYKDARVCS 588
           +KGY CR GK+YK+ARVC+
Sbjct: 568 AKGYLCRPGKVYKEARVCT 582

[Close]
BLAST of CSPI03G07140 vs. TAIR 10
Match: AT2G46740.1 (D-arabinono-1,4-lactone oxidase family protein )

HSP 1 Score: 728.0 bits (1878), Expect = 6.1e-210
Identity = 346/585 (59.15%), Postives = 435/585 (74.36%), Query Frame = 0 

Query: 6   WRSYCVVSCPSSVVFMFLVLVSECSPPGDPIKCVSVSPNKYANCTITNFYGSFPDRSICR 65
           WR+   + C  ++V   +      +PP DP+KCVS +     NC +TN  G+FPDRS CR
Sbjct: 13  WRTLLGLYCLFTLVHTVI-----STPPEDPVKCVSGN----TNCIVTNSLGAFPDRSTCR 72

Query: 66  VAEVIYPTTEAEVMLAVAAATRGLRKMKVGTRFSHSIPKLACPEGEEGVVISTKYLNRTV 125
            A V YPTTEAE++  VAAAT+  RKM+V TR+SHSIPKL C +G +G+ ISTK+LN TV
Sbjct: 73  AANVAYPTTEAELVSIVAAATKAGRKMRVTTRYSHSIPKLTCTDGNDGLFISTKFLNHTV 132

Query: 126 KVDVEGMSISVEGGATLRQIIEDAATAGLALPSTPYWWGLTIGGLLSTGAHGSSLWGKGS 185
           + D + M+++VE G TLRQ+I +AA  GLALP  PYWWG+T+GG++ TGAHGSSLWGKGS
Sbjct: 133 QADAKAMTLTVESGVTLRQLIAEAAKVGLALPYAPYWWGVTVGGMMGTGAHGSSLWGKGS 192

Query: 186 AVHDYVVAVQMVSPGGPEDGYAKQRTIL-PDDQNHLNAAKVSLGVLGVITQVTLKLQPMF 245
           AVHDYV  ++MVSPG   DG+AK R +      N  NAAKVSLGVLGVI+QVT +LQPMF
Sbjct: 193 AVHDYVTEIRMVSPGSVNDGFAKIRVLSETTTPNEFNAAKVSLGVLGVISQVTFELQPMF 252

Query: 246 KRSITYLKRDDKDLGDEVIRFGKSYEFGDMNWYPSQRKVVYRIDTRVPSNTSGNGLYDFI 305
           KRS+ Y+ R+D D  DE + FGK +EF D  W PSQ KVVYR+D RV  NT GNGLYDF 
Sbjct: 253 KRSLKYVMRNDLDFNDEALTFGKKHEFADFVWLPSQGKVVYRMDDRVAVNTLGNGLYDFF 312

Query: 306 PFRPTLSVELALLRASEEEQESRRDAAAKCLSGNLITSTLSLLAYGLTNNGITFLKYPVI 365
           PFR  LS  LA  R+SEE QE+ RDA  KC++   I+STL   +YGLTNNGITF  YPVI
Sbjct: 313 PFRSQLSAVLATTRSSEETQETLRDAHGKCVTATTISSTLFSTSYGLTNNGITFTGYPVI 372

Query: 366 GYNNRLQSSGSCLDSLNDFRITACPWDPLIKGEFFYETSVSIELTVVKSFIEDVQKLAEL 425
           G  NR+ SSGSCLD L D   +AC WD  +KG F+++T+ SI LT VKSFI D++ L ++
Sbjct: 373 GSQNRMMSSGSCLDGLEDKLTSACAWDSRVKGVFYHQTTFSIPLTQVKSFINDIKSLLKI 432

Query: 426 EPMAFCGLELYNGILMRYVTASTAYLGKQQDSVEFDLTYYRSKDPMSPRLFEDVIEEVEQ 485
           +  + CGLELY GILMRYVT+S AYLGK+ ++++FD+TYYR+ DP++PRL+ED IEE+EQ
Sbjct: 433 DSKSLCGLELYYGILMRYVTSSPAYLGKETEAIDFDITYYRANDPLTPRLYEDFIEEIEQ 492

Query: 486 MAVFKYGGIPHWGKNRNVAFHQVFHKYRNIEKFLKIMKEYDPHRLFSNEWTDQILGVEGT 545
           +A+ KY  +PHWGKNRN+AF  V  KY+N   FLK+ + YDP+ LFS+EWTDQILG++G 
Sbjct: 493 IALLKYNALPHWGKNRNLAFDGVIKKYKNAPAFLKVKESYDPNGLFSSEWTDQILGIKGN 552

Query: 546 VNIYKDGCALEGLCICSKHTHCAPSKGYFCRAGKIYKDARVCSYL 590
             I KDGCALEGLCICS   HCAPSKGY CR GK+YK+ARVC+++
Sbjct: 553 PTIVKDGCALEGLCICSDDAHCAPSKGYLCRPGKVYKEARVCTHV 588

[Close]
BLAST of CSPI03G07140 vs. TAIR 10
Match: AT1G32300.1 (D-arabinono-1,4-lactone oxidase family protein )

HSP 1 Score: 712.6 bits (1838), Expect = 2.6e-205
Identity = 338/579 (58.38%), Postives = 432/579 (74.61%), Query Frame = 0 

Query: 17  SVVFMFLVLVSECSPPGDPIKCVSVSPNKYANCTITNFYGSFPDRSICRVAEVIYPTTEA 76
           S++F      +  +PP DP+KC S +      CT+TN YG+FPDRSIC  A+V YP TEA
Sbjct: 6   SLIFFCFCTFASSTPPDDPVKCESGN----NMCTVTNSYGAFPDRSICEAAKVEYPKTEA 65

Query: 77  EVMLAVAAATRGLRKMKVGTRFSHSIPKLACPEGEEGVVISTKYLNRTVKVDVEGMSISV 136
           E++  VAAATR  +K++V TR+ HSIPKL C +G++GV+ISTK+LN  V  + E  +++V
Sbjct: 66  ELVSIVAAATRAGQKVRVVTRYVHSIPKLVCTDGKDGVLISTKFLNNVVGTNPEAKTLTV 125

Query: 137 EGGATLRQIIEDAATAGLALPSTPYWWGLTIGGLLSTGAHGSSLWGKGSAVHDYVVAVQM 196
           E G TLRQ+I +AA   LALP  PYWWGLT+GGL+ TGAHGSSLWGKGSAVHDYV  ++M
Sbjct: 126 ESGVTLRQLIGEAAELELALPHAPYWWGLTVGGLMGTGAHGSSLWGKGSAVHDYVSEIRM 185

Query: 197 VSPGGPEDGYAKQRTILPD-DQNHLNAAKVSLGVLGVITQVTLKLQPMFKRSITYLKRDD 256
           VSPG   DGY K R +    D +   AAKVSLGVLGVI+QVT +LQPMFKRS+T++ ++D
Sbjct: 186 VSPGLASDGYVKVRVLSETIDPDEFRAAKVSLGVLGVISQVTFQLQPMFKRSLTFVMQND 245

Query: 257 KDLGDEVIRFGKSYEFGDMNWYPSQRKVVYRIDTRVPSNTSGNGLYDFIPFRPTLSVELA 316
            D GD+ + FG+ +EF D  W PSQ KVVYR+D RVP NTSGNGL+DF PFRP LSV LA
Sbjct: 246 SDFGDQAVTFGEKHEFADFLWLPSQGKVVYRMDDRVPVNTSGNGLFDFFPFRPQLSVALA 305

Query: 317 LLRASEEEQESRRDAAAKCLSGNLITSTLSLLAYGLTNNGITFLKYPVIGYNNRLQSSGS 376
           ++R+ EE +ES  DA  KC     ITS L  ++YG+TNNG+ F  YPVIG  N + SSG+
Sbjct: 306 IIRSLEESEESSGDANDKCARAEQITSFLFSISYGVTNNGMEFTGYPVIGKQNHMMSSGT 365

Query: 377 CLDSLNDFRITACPWDPLIKGEFFYETSVSIELTVVKSFIEDVQKLAELEPMAFCGLELY 436
           CLDS  D  IT+CPWDP IKG+FF++T+ SI LT VK FI D++ L ++EP + C LE  
Sbjct: 366 CLDSHQDGLITSCPWDPRIKGQFFHQTAFSIPLTRVKGFINDIKALVKIEPKSLCALERS 425

Query: 437 NGILMRYVTASTAYLGKQQDSVEFDLTYYRSK-DPMSPRLFEDVIEEVEQMAVFKYGGIP 496
           NGIL+RYVT+S A+LGK++ +++FDLTYYRSK DP++PRL+ED IEE+EQMA+FKY  +P
Sbjct: 426 NGILIRYVTSSPAFLGKEEKALDFDLTYYRSKDDPLAPRLYEDFIEEIEQMAIFKYNALP 485

Query: 497 HWGKNRNVAFHQVFHKYRNIEKFLKIMKEYDPHRLFSNEWTDQILGVEGTVNIYKDGCAL 556
           HWGKNRN+AF  V  KY+N   FLK+ + +DP  LFS EWT+QILG++G V I K+GCAL
Sbjct: 486 HWGKNRNLAFDGVIRKYKNANTFLKVKERFDPLGLFSTEWTNQILGLKGNVTIVKEGCAL 545

Query: 557 EGLCICSKHTHCAPSKGYFCRAGKIYKDARVCSYLQSPN 594
           EGLC+CS   HCAP KGY CR GK+Y  ARVC++++S N
Sbjct: 546 EGLCVCSDDAHCAPKKGYLCRPGKVYTKARVCTHVKSVN 580

[Close]
BLAST of CSPI03G07140 vs. TAIR 10
Match: AT5G56490.1 (D-arabinono-1,4-lactone oxidase family protein )

HSP 1 Score: 709.1 bits (1829), Expect = 2.9e-204
Identity = 335/579 (57.86%), Postives = 435/579 (75.13%), Query Frame = 0 

Query: 17  SVVFMFLVLVSECSPPGDPIKCVSVSPNKYAN--CTITNFYGSFPDRSICRVAEVIYPTT 76
           S++F F    S  SPP DP+ C      ++ N  CT+TN YG+FPDRSIC+ A+V YP T
Sbjct: 6   SLIFCFFTFASS-SPPDDPVNC------EFGNTMCTVTNSYGAFPDRSICKAAKVEYPRT 65

Query: 77  EAEVMLAVAAATRGLRKMKVGTRFSHSIPKLACPEGEEGVVISTKYLNRTVKVDVEGMSI 136
           EAE++  VAAATR  +KM+V TR+SHS PKL C +G++G +ISTK+LN  V  + E  ++
Sbjct: 66  EAELVSIVAAATRAGQKMRVVTRYSHSFPKLVCTDGKDGTLISTKFLNHVVTTNPEAKTL 125

Query: 137 SVEGGATLRQIIEDAATAGLALPSTPYWWGLTIGGLLSTGAHGSSLWGKGSAVHDYVVAV 196
           +VE G TLRQ+IE+AA   LALP  PYWWGLT+GG++ TGAHGSSLWGKGSAVHDYV  +
Sbjct: 126 TVESGVTLRQLIEEAAKFDLALPYAPYWWGLTVGGMMGTGAHGSSLWGKGSAVHDYVTEI 185

Query: 197 QMVSPGGPEDGYAKQRTILPD-DQNHLNAAKVSLGVLGVITQVTLKLQPMFKRSITYLKR 256
           ++VSPG   DGY K + +    D     AAKVSLGVLGVI+QVT +LQPMFKRS+ Y+ R
Sbjct: 186 RLVSPGLASDGYVKVQVLSETIDPEEFRAAKVSLGVLGVISQVTFELQPMFKRSLNYVMR 245

Query: 257 DDKDLGDEVIRFGKSYEFGDMNWYPSQRKVVYRIDTRVPSNTSGNGLYDFIPFRPTLSVE 316
           +D D GD+ + FG+ +EF D  W PSQ KVVYR+D RVP NTSG+GL++F PFR  LS+ 
Sbjct: 246 NDSDFGDQAVSFGERHEFADFLWLPSQGKVVYRMDGRVPLNTSGDGLFEFFPFRSQLSLV 305

Query: 317 LALLRASEEEQESRRDAAAKCLSGNLITSTLSLLAYGLTNNGITFLKYPVIGYNNRLQSS 376
           LA+ R+ EE +ES  DA  KC+   L++S++ L++YG+TNNG+ F  YPVIG  N + SS
Sbjct: 306 LAIDRSLEESEESLEDANMKCVRAKLVSSSMFLMSYGVTNNGLIFTGYPVIGMQNHMMSS 365

Query: 377 GSCLDSLNDFRITACPWDPLIKGEFFYETSVSIELTVVKSFIEDVQKLAELEPMAFCGLE 436
           GSCLDS  D  ITACPWDP IKG+FF++T+ S+ LT VKSFI D++ L ++EP + C LE
Sbjct: 366 GSCLDSRQDGLITACPWDPRIKGQFFHQTTFSVSLTNVKSFINDIKALVKIEPKSLCVLE 425

Query: 437 LYNGILMRYVTASTAYLGKQQDSVEFDLTYYRSK-DPMSPRLFEDVIEEVEQMAVFKYGG 496
             NGIL+RYVT+S A+LGK++ +++FDLTYYRSK DP++PRL+ED IEE+EQMA+FKY  
Sbjct: 426 GSNGILIRYVTSSLAFLGKEEKALDFDLTYYRSKNDPLAPRLYEDYIEEIEQMAIFKYNA 485

Query: 497 IPHWGKNRNVAFHQVFHKYRNIEKFLKIMKEYDPHRLFSNEWTDQILGVEGTVNIYKDGC 556
           +PHWGKNRN+AF     KY+N   FLK+ +++D   LFS EWTDQILG++G V I K GC
Sbjct: 486 LPHWGKNRNLAFDGAIRKYKNANAFLKVKEKFDSLGLFSTEWTDQILGLKGNVTIVKQGC 545

Query: 557 ALEGLCICSKHTHCAPSKGYFCRAGKIYKDARVCSYLQS 592
           ALEGLCICS+ +HCAP+KGY CR GK+Y++ARVC+ + S
Sbjct: 546 ALEGLCICSEDSHCAPTKGYLCRPGKVYREARVCTRVSS 577

The following BLAST results are available for this feature:
BLAST of CSPI03G07140 vs. ExPASy Swiss-Prot
Match NameE-valueIdentityDescription
O810322.6e-21862.09Probable L-gulonolactone oxidase 6 OS=Arabidopsis thaliana OX=3702 GN=GULLO6 PE=... [more]
Q6NQ661.4e-21162.08L-gulonolactone oxidase 2 OS=Arabidopsis thaliana OX=3702 GN=GULLO2 PE=1 SV=1[more]
O810308.5e-20959.15L-gulonolactone oxidase 5 OS=Arabidopsis thaliana OX=3702 GN=GULLO5 PE=1 SV=1[more]
Q9C6143.7e-20458.38Probable L-gulonolactone oxidase 1 OS=Arabidopsis thaliana OX=3702 GN=GULLO1 PE=... [more]
Q9FM824.1e-20357.86Probable L-gulonolactone oxidase 4 OS=Arabidopsis thaliana OX=3702 GN=GULLO4 PE=... [more]
BLAST of CSPI03G07140 vs. ExPASy TrEMBL
Match NameE-valueIdentityDescription
A0A0A0L3K40.0e+0096.90L-gulonolactone oxidase OS=Cucumis sativus OX=3659 GN=Csa_3G116800 PE=3 SV=1[more]
A0A1S3AUG30.0e+0091.44L-gulonolactone oxidase OS=Cucumis melo OX=3656 GN=LOC103483108 PE=3 SV=1[more]
A0A6J1FUH81.8e-26278.21L-gulonolactone oxidase OS=Cucurbita moschata OX=3662 GN=LOC111448536 PE=3 SV=1[more]
A0A6J1IG872.9e-26077.43L-gulonolactone oxidase OS=Cucurbita maxima OX=3661 GN=LOC111474382 PE=3 SV=1[more]
A0A4U5PX321.1e-23065.34L-gulonolactone oxidase OS=Populus alba OX=43335 GN=D5086_0000167310 PE=3 SV=1[more]
BLAST of CSPI03G07140 vs. NCBI nr
Match NameE-valueIdentityDescription
XP_004134280.10.0e+0099.66probable L-gulonolactone oxidase 6 [Cucumis sativus] >KAE8650211.1 hypothetical ... [more]
XP_008437774.10.0e+0091.44PREDICTED: probable L-gulonolactone oxidase 6 [Cucumis melo][more]
XP_038882597.18.0e-29783.59LOW QUALITY PROTEIN: probable L-gulonolactone oxidase 6 [Benincasa hispida][more]
XP_023540226.15.2e-26479.09probable L-gulonolactone oxidase 6 [Cucurbita pepo subsp. pepo][more]
XP_022943972.13.8e-26278.21probable L-gulonolactone oxidase 6 [Cucurbita moschata][more]
BLAST of CSPI03G07140 vs. TAIR 10
Match NameE-valueIdentityDescription
AT2G46760.11.9e-21962.09D-arabinono-1,4-lactone oxidase family protein [more]
AT2G46750.11.0e-21262.08D-arabinono-1,4-lactone oxidase family protein [more]
AT2G46740.16.1e-21059.15D-arabinono-1,4-lactone oxidase family protein [more]
AT1G32300.12.6e-20558.38D-arabinono-1,4-lactone oxidase family protein [more]
AT5G56490.12.9e-20457.86D-arabinono-1,4-lactone oxidase family protein [more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (PI 183967) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR007173D-arabinono-1,4-lactone oxidasePFAMPF04030ALOcoord: 438..537
e-value: 3.1E-11
score: 43.5
IPR010030L-gulonolactone oxidase, plantTIGRFAMTIGR01677TIGR01677coord: 31..586
e-value: 2.2E-254
score: 843.1
NoneNo IPR availableGENE3D3.30.70.2520coord: 400..501
e-value: 8.7E-6
score: 28.0
NoneNo IPR availablePANTHERPTHR13878:SF67L-GULONOLACTONE OXIDASE 5coord: 23..589
NoneNo IPR availablePANTHERPTHR13878GULONOLACTONE OXIDASEcoord: 23..589
IPR006094FAD linked oxidase, N-terminalPFAMPF01565FAD_binding_4coord: 69..201
e-value: 3.1E-15
score: 56.1
IPR016169FAD-binding, type PCMH, subdomain 2GENE3D3.30.465.10coord: 122..242
e-value: 3.4E-29
score: 103.0
IPR016166FAD-binding domain, PCMH-typePROSITEPS51387FAD_PCMHcoord: 62..243
score: 19.767622
IPR036318FAD-binding, type PCMH-like superfamilySUPERFAMILY56176FAD-binding/transporter-associated domain-likecoord: 53..242

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CSPI03G07140.1CSPI03G07140.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0019853 L-ascorbic acid biosynthetic process
cellular_component GO:0016020 membrane
molecular_function GO:0003885 D-arabinono-1,4-lactone oxidase activity
molecular_function GO:0071949 FAD binding
molecular_function GO:0050105 L-gulonolactone oxidase activity
molecular_function GO:0050660 flavin adenine dinucleotide binding
molecular_function GO:0016491 oxidoreductase activity