CSPI03G02720 (gene) Cucumber (PI 183967) v1

Overview


Name	CSPI03G02720
Type	gene
Organism	Cucumis sativus var. hardwickii cv. PI 183967 (Cucumber (PI 183967) v1)
Description	Eukaryotic aspartyl protease family protein
Location	Chr3: 2104705 .. 2106775 (+)
RNA-Seq Expression	CSPI03G02720
Synteny	CSPI03G02720

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDSpolypeptidethree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

AAAATATGAACCAAACTTTTAAATTTTTTTAAGGAAAACTAAGCTTATAATTTAAGAAGATAAAGAAAGAAGAAAGAAGAAAGAAGAAATAAGAAAGAGGGTGTAGTTGTAATGGTCACCACTTTTGAAACCCATACAAAAACCCCATCTTTCTCGTCTTCTAACCAGAACCTTCTTCTTCTTCTTCTATTCTCAATCCCCATTTCTTTAAATTCCCCAGTTTCTATCTCTAATTTCATTTCAAAAACCCCAATTTCTCTATCTAAAGTACTCCATTAATGGCGGTTTCCCCTGTTTTCATCTTCCTCCTTTGTTTTCTCCTCTCCTCCCCTGTTTTCTCCTCACAAATTTTCCTTCTACCTCTCTCCCATTCCTTATCATCCTCAATCTCCGATTTCAACAACACCCACAATCTTCTCAAATCCACTGCCACCCGCTCCTCCGCCCGATTCCACCGCCACCGCCATAACCACCTCTCTCTGCCCCTCTCCCCTGGCGGCGATTACACTCTCTCCTTCAACCTCGGCTCTGAGTCTCACAAAATTTCCCTCTATATGGACACTGGCAGCGACCTCGTTTGGTTCCCTTGTTCCCCGTTTGAGTGTATTCTTTGTGAAGGTAAACCAAAAATTCAATCCCCTTTGCCCAAAATCGCAAATAACAAATCAGTTTCCTGCAGCGCCGCCGCCTGCTCCGCCGCTCACGGTGGCTCCCTCTCCGCTTCCCACCTCTGTGCAATTTCTCGATGTCCACTTGAATCCATTGAAATTTCTGAGTGCTCCTCTTTTTCCTGTCCTCCGTTTTATTATGCTTATGGCGATGGGAGTTTGGTTGCTCGGCTTTATAGAGATAGCCTCAGCTTGCCAACGCCGGCGCCATCTCCGCCTATTAATGTTCGGAATTTTACTTTTGGATGTGCCCACACGACGCTTGGCGAGCCGGTTGGGGTTGCCGGATTCGGCCGGGGGGTGTTGTCAATGCCCAGTCAACTCGCTACTTTCTCCCCTCAACTCGGGAACCGGTTTTCTTATTGTTTGGTTTCTCACTCGTTTGCGGCAGACCGAGTTCGCCGCCCGAGTCCACTGATTCTCGGGCGGTACTACACCGGGGAGACGGAGTTCATTTACACTTCCTTGCTTGAGAATCCAAAACATCCTTACTTTTACTCCGTTGGGTTGGCCGGAATATCAGTTGGGAATGTAAGGATTCCAGCGCCGGAGTTTCTGACAAAAGTGGATGAGGGTGGGAGTGGCGGCGTTGTGGTGGATTCCGGCACTACTTTTACTATGCTCCCGGCAGGTTTGTATGAATCGGTGGTGGCCGAGTTCGAAAACCGTACCGGAAAGGTTGCAAACCGGGCGAGACGGATTGAAGAAAATACCGGGTTGAGCCCTTGCTATTACTACGAGAACTCAGTTGGGGTGCCACGTGTCGTGCTACATTTTGTTGGGGAAAAATCCAATGTGGTGCTTCCTAGGAAGAACTATTTCTACGAGTTTTTGGACGGCGGAGATGGGGTGGTGGGGAGGAAGAGAAAAGTTGGATGTTTGATGTTGATGAACGGTGGAGATGAGGCTGAGCTGGCAGGTGGGCCTGGTGCGACGCTTGGGAACTACCAACAACAAGGTTTTGAGGTGGTTTATGATTTGGAAAAGAACCGGGTTGGATTCGCTCGGCGGCAATGCTCCACTCTTTGGGACAATTTGAACCGGAGTAAGTGAAAGTGTGAACCCGGTTGAGGAAGTGAGAGTTGATCTTTGACATTGTGAGTATTGTCAACGGTGAAGGGTATGAGGGTAAATAAGGTAATTTTAGGTTTCAAGGGTTTATTTATTTATTTTATTCTATTTTTTGTTGTAAATTCCTTGGGCACTTCACTTCTTGCTTTAATCTTATTTTTACTTGAAATTTGTATATTAAAAGTGTTCAAAAAAAAAATGTATAGCTCAAATTTTATTGCATGAAGCAAGGAATGAAGTGGTTGCTTTTATCAAGTTAAGAATAAAATAGGGGCAATAAATTAAGGATGTTAAAAAACAAATCATAAATCAAATTTACTTGTAAAACTTTT

mRNA sequence

AAAATATGAACCAAACTTTTAAATTTTTTTAAGGAAAACTAAGCTTATAATTTAAGAAGATAAAGAAAGAAGAAAGAAGAAAGAAGAAATAAGAAAGAGGGTGTAGTTGTAATGGTCACCACTTTTGAAACCCATACAAAAACCCCATCTTTCTCGTCTTCTAACCAGAACCTTCTTCTTCTTCTTCTATTCTCAATCCCCATTTCTTTAAATTCCCCAGTTTCTATCTCTAATTTCATTTCAAAAACCCCAATTTCTCTATCTAAAGTACTCCATTAATGGCGGTTTCCCCTGTTTTCATCTTCCTCCTTTGTTTTCTCCTCTCCTCCCCTGTTTTCTCCTCACAAATTTTCCTTCTACCTCTCTCCCATTCCTTATCATCCTCAATCTCCGATTTCAACAACACCCACAATCTTCTCAAATCCACTGCCACCCGCTCCTCCGCCCGATTCCACCGCCACCGCCATAACCACCTCTCTCTGCCCCTCTCCCCTGGCGGCGATTACACTCTCTCCTTCAACCTCGGCTCTGAGTCTCACAAAATTTCCCTCTATATGGACACTGGCAGCGACCTCGTTTGGTTCCCTTGTTCCCCGTTTGAGTGTATTCTTTGTGAAGGTAAACCAAAAATTCAATCCCCTTTGCCCAAAATCGCAAATAACAAATCAGTTTCCTGCAGCGCCGCCGCCTGCTCCGCCGCTCACGGTGGCTCCCTCTCCGCTTCCCACCTCTGTGCAATTTCTCGATGTCCACTTGAATCCATTGAAATTTCTGAGTGCTCCTCTTTTTCCTGTCCTCCGTTTTATTATGCTTATGGCGATGGGAGTTTGGTTGCTCGGCTTTATAGAGATAGCCTCAGCTTGCCAACGCCGGCGCCATCTCCGCCTATTAATGTTCGGAATTTTACTTTTGGATGTGCCCACACGACGCTTGGCGAGCCGGTTGGGGTTGCCGGATTCGGCCGGGGGGTGTTGTCAATGCCCAGTCAACTCGCTACTTTCTCCCCTCAACTCGGGAACCGGTTTTCTTATTGTTTGGTTTCTCACTCGTTTGCGGCAGACCGAGTTCGCCGCCCGAGTCCACTGATTCTCGGGCGGTACTACACCGGGGAGACGGAGTTCATTTACACTTCCTTGCTTGAGAATCCAAAACATCCTTACTTTTACTCCGTTGGGTTGGCCGGAATATCAGTTGGGAATGTAAGGATTCCAGCGCCGGAGTTTCTGACAAAAGTGGATGAGGGTGGGAGTGGCGGCGTTGTGGTGGATTCCGGCACTACTTTTACTATGCTCCCGGCAGGTTTGTATGAATCGGTGGTGGCCGAGTTCGAAAACCGTACCGGAAAGGTTGCAAACCGGGCGAGACGGATTGAAGAAAATACCGGGTTGAGCCCTTGCTATTACTACGAGAACTCAGTTGGGGTGCCACGTGTCGTGCTACATTTTGTTGGGGAAAAATCCAATGTGGTGCTTCCTAGGAAGAACTATTTCTACGAGTTTTTGGACGGCGGAGATGGGGTGGTGGGGAGGAAGAGAAAAGTTGGATGTTTGATGTTGATGAACGGTGGAGATGAGGCTGAGCTGGCAGGTGGGCCTGGTGCGACGCTTGGGAACTACCAACAACAAGGTTTTGAGGTGGTTTATGATTTGGAAAAGAACCGGGTTGGATTCGCTCGGCGGCAATGCTCCACTCTTTGGGACAATTTGAACCGGAGTAAGTGAAAGTGTGAACCCGGTTGAGGAAGTGAGAGTTGATCTTTGACATTGTGAGTATTGTCAACGGTGAAGGGTATGAGGGTAAATAAGGTAATTTTAGGTTTCAAGGGTTTATTTATTTATTTTATTCTATTTTTTGTTGTAAATTCCTTGGGCACTTCACTTCTTGCTTTAATCTTATTTTTACTTGAAATTTGTATATTAAAAGTGTTCAAAAAAAAAATGTATAGCTCAAATTTTATTGCATGAAGCAAGGAATGAAGTGGTTGCTTTTATCAAGTTAAGAATAAAATAGGGGCAATAAATTAAGGATGTTAAAAAACAAATCATAAATCAAATTTACTTGTAAAACTTTT

Coding sequence (CDS)

ATGGCGGTTTCCCCTGTTTTCATCTTCCTCCTTTGTTTTCTCCTCTCCTCCCCTGTTTTCTCCTCACAAATTTTCCTTCTACCTCTCTCCCATTCCTTATCATCCTCAATCTCCGATTTCAACAACACCCACAATCTTCTCAAATCCACTGCCACCCGCTCCTCCGCCCGATTCCACCGCCACCGCCATAACCACCTCTCTCTGCCCCTCTCCCCTGGCGGCGATTACACTCTCTCCTTCAACCTCGGCTCTGAGTCTCACAAAATTTCCCTCTATATGGACACTGGCAGCGACCTCGTTTGGTTCCCTTGTTCCCCGTTTGAGTGTATTCTTTGTGAAGGTAAACCAAAAATTCAATCCCCTTTGCCCAAAATCGCAAATAACAAATCAGTTTCCTGCAGCGCCGCCGCCTGCTCCGCCGCTCACGGTGGCTCCCTCTCCGCTTCCCACCTCTGTGCAATTTCTCGATGTCCACTTGAATCCATTGAAATTTCTGAGTGCTCCTCTTTTTCCTGTCCTCCGTTTTATTATGCTTATGGCGATGGGAGTTTGGTTGCTCGGCTTTATAGAGATAGCCTCAGCTTGCCAACGCCGGCGCCATCTCCGCCTATTAATGTTCGGAATTTTACTTTTGGATGTGCCCACACGACGCTTGGCGAGCCGGTTGGGGTTGCCGGATTCGGCCGGGGGGTGTTGTCAATGCCCAGTCAACTCGCTACTTTCTCCCCTCAACTCGGGAACCGGTTTTCTTATTGTTTGGTTTCTCACTCGTTTGCGGCAGACCGAGTTCGCCGCCCGAGTCCACTGATTCTCGGGCGGTACTACACCGGGGAGACGGAGTTCATTTACACTTCCTTGCTTGAGAATCCAAAACATCCTTACTTTTACTCCGTTGGGTTGGCCGGAATATCAGTTGGGAATGTAAGGATTCCAGCGCCGGAGTTTCTGACAAAAGTGGATGAGGGTGGGAGTGGCGGCGTTGTGGTGGATTCCGGCACTACTTTTACTATGCTCCCGGCAGGTTTGTATGAATCGGTGGTGGCCGAGTTCGAAAACCGTACCGGAAAGGTTGCAAACCGGGCGAGACGGATTGAAGAAAATACCGGGTTGAGCCCTTGCTATTACTACGAGAACTCAGTTGGGGTGCCACGTGTCGTGCTACATTTTGTTGGGGAAAAATCCAATGTGGTGCTTCCTAGGAAGAACTATTTCTACGAGTTTTTGGACGGCGGAGATGGGGTGGTGGGGAGGAAGAGAAAAGTTGGATGTTTGATGTTGATGAACGGTGGAGATGAGGCTGAGCTGGCAGGTGGGCCTGGTGCGACGCTTGGGAACTACCAACAACAAGGTTTTGAGGTGGTTTATGATTTGGAAAAGAACCGGGTTGGATTCGCTCGGCGGCAATGCTCCACTCTTTGGGACAATTTGAACCGGAGTAAGTGA

Protein sequence

MAVSPVFIFLLCFLLSSPVFSSQIFLLPLSHSLSSSISDFNNTHNLLKSTATRSSARFHRHRHNHLSLPLSPGGDYTLSFNLGSESHKISLYMDTGSDLVWFPCSPFECILCEGKPKIQSPLPKIANNKSVSCSAAACSAAHGGSLSASHLCAISRCPLESIEISECSSFSCPPFYYAYGDGSLVARLYRDSLSLPTPAPSPPINVRNFTFGCAHTTLGEPVGVAGFGRGVLSMPSQLATFSPQLGNRFSYCLVSHSFAADRVRRPSPLILGRYYTGETEFIYTSLLENPKHPYFYSVGLAGISVGNVRIPAPEFLTKVDEGGSGGVVVDSGTTFTMLPAGLYESVVAEFENRTGKVANRARRIEENTGLSPCYYYENSVGVPRVVLHFVGEKSNVVLPRKNYFYEFLDGGDGVVGRKRKVGCLMLMNGGDEAELAGGPGATLGNYQQQGFEVVYDLEKNRVGFARRQCSTLWDNLNRSK*

Homology

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BLAST of CSPI03G02720 vs. ExPASy Swiss-Prot
Match: Q940R4 (Probable aspartyl protease At4g16563 OS=Arabidopsis thaliana OX=3702 GN=At4g16563 PE=2 SV=1)

HSP 1 Score: 581.6 bits (1498), Expect = 8.0e-165
Identity = 298/478 (62.34%), Postives = 356/478 (74.48%), Query Frame = 0


Query: 26  LLPLSHSLSSSISDFNNTHNLLKSTATRSSARFHRHRH----NHLSLPLSPGGDYTLSFN 85
           LL LSHSLS+S    +  H LLKS+++RSSARF RH H      LSLP+S G DY +S +
Sbjct: 30  LLHLSHSLSTSKHSSSPLH-LLKSSSSRSSARFRRHHHKQQQQQLSLPISSGSDYLISLS 89

Query: 86  LGSESHKISLYMDTGSDLVWFPCSPFECILCEGKPKIQSPLPKIANN-KSVSCSAAACSA 145
           +GS S  +SLY+DTGSDLVWFPC PF CILCE KP   SP   ++++  +VSCS+ +CSA
Sbjct: 90  VGSSSSAVSLYLDTGSDLVWFPCRPFTCILCESKPLPPSPPSSLSSSATTVSCSSPSCSA 149

Query: 146 AHGGSLSASHLCAISRCPLESIEISEC--SSFSCPPFYYAYGDGSLVARLYRDSLSLPTP 205
           AH  SL +S LCAIS CPL+ IE  +C  SS+ CPPFYYAYGDGSLVA+LY DSLSLP+ 
Sbjct: 150 AH-SSLPSSDLCAISNCPLDFIETGDCNTSSYPCPPFYYAYGDGSLVAKLYSDSLSLPS- 209

Query: 206 APSPPINVRNFTFGCAHTTLGEPVGVAGFGRGVLSMPSQLATFSPQLGNRFSYCLVSHSF 265
                ++V NFTFGCAHTTL EP+GVAGFGRG LS+P+QLA  SP LGN FSYCLVSHSF
Sbjct: 210 -----VSVSNFTFGCAHTTLAEPIGVAGFGRGRLSLPAQLAVHSPHLGNSFSYCLVSHSF 269

Query: 266 AADRVRRPSPLILGRYY------TGET--------------EFIYTSLLENPKHPYFYSV 325
            +DRVRRPSPLILGR+        G T              EF++T +LENPKHPYFYSV
Sbjct: 270 DSDRVRRPSPLILGRFVDKKEKRVGTTDDHDDGDDEKKKKNEFVFTEMLENPKHPYFYSV 329

Query: 326 GLAGISVGNVRIPAPEFLTKVDEGGSGGVVVDSGTTFTMLPAGLYESVVAEFENRTGKVA 385
            L GIS+G   IPAP  L ++D+ G GGVVVDSGTTFTMLPA  Y SVV EF++R G+V 
Sbjct: 330 SLQGISIGKRNIPAPAMLRRIDKNGGGGVVVDSGTTFTMLPAKFYNSVVEEFDSRVGRVH 389

Query: 386 NRARRIEENTGLSPCYYYENSVGVPRVVLHFVGEKSNVVLPRKNYFYEFLDGGDGVVGRK 445
            RA R+E ++G+SPCYY   +V VP +VLHF G +S+V LPR+NYFYEF+DGGDG    K
Sbjct: 390 ERADRVEPSSGMSPCYYLNQTVKVPALVLHFAGNRSSVTLPRRNYFYEFMDGGDG-KEEK 449

Query: 446 RKVGCLMLMNGGDEAELAGGPGATLGNYQQQGFEVVYDLEKNRVGFARRQCSTLWDNL 477
           RK+GCLMLMNGGDE+EL GG GA LGNYQQQGFEVVYDL   RVGFA+R+C++LWD+L
Sbjct: 450 RKIGCLMLMNGGDESELRGGTGAILGNYQQQGFEVVYDLLNRRVGFAKRKCASLWDSL 498

[Close]

BLAST of CSPI03G02720 vs. ExPASy Swiss-Prot
Match: Q9LNJ3 (Aspartyl protease family protein 2 OS=Arabidopsis thaliana OX=3702 GN=APF2 PE=2 SV=1)

HSP 1 Score: 152.1 bits (383), Expect = 1.6e-35
Identity = 133/409 (32.52%), Postives = 183/409 (44.74%), Query Frame = 0


Query: 70  LSPG-GDYTLSFNLGSESHKISLYMDTGSDLVWFPCSPFECILCEGKPKIQSPLPKIANN 129
           LS G G+Y     +G+ +  + + +DTGSD+VW  C+P  C  C  +       P     
Sbjct: 135 LSQGSGEYFTRLGVGTPARYVYMVLDTGSDIVWLQCAP--CRRCYSQSD-----PIFDPR 194

Query: 130 KSVSCSAAACSAAHGGSLSASHLCAISRCPLESIEISECSSFSCPPFYYAYGDGSL-VAR 189
           KS + +   CS+ H   L ++  C   R          C       +  +YGDGS  V  
Sbjct: 195 KSKTYATIPCSSPHCRRLDSAG-CNTRR--------KTCL------YQVSYGDGSFTVGD 254

Query: 190 LYRDSLSLPTPAPSPPINVRNFTFGCAHTTLGEPVGVA---GFGRGVLSMPSQLATFSPQ 249
              ++L+           V+    GC H   G  VG A   G G+G LS P Q      +
Sbjct: 255 FSTETLTFRRN------RVKGVALGCGHDNEGLFVGAAGLLGLGKGKLSFPGQT---GHR 314

Query: 250 LGNRFSYCLVSHSFAADRVRRPSPLILGRYYTGETEFIYTSLLENPKHPYFYSVGLAGIS 309
              +FSYCLV  S ++    +PS ++ G          +T LL NPK   FY VGL GIS
Sbjct: 315 FNQKFSYCLVDRSASS----KPSSVVFGNAAVSRIA-RFTPLLSNPKLDTFYYVGLLGIS 374

Query: 310 VGNVRIP-APEFLTKVDEGGSGGVVVDSGTTFTMLPAGLYESVVAEFENRTGKVANRARR 369
           VG  R+P     L K+D+ G+GGV++DSGT+ T L    Y ++   F  R G  A   +R
Sbjct: 375 VGGTRVPGVTASLFKLDQIGNGGVIIDSGTSVTRLIRPAYIAMRDAF--RVG--AKTLKR 434

Query: 370 IEENTGLSPCYYYE--NSVGVPRVVLHFVGEKSNVVLPRKNYFYEFLDGGDGVVGRKRKV 429
             + +    C+     N V VP VVLHF G  ++V LP  NY       G         +
Sbjct: 435 APDFSLFDTCFDLSNMNEVKVPTVVLHFRG--ADVSLPATNYLIPVDTNGKFCFAFAGTM 485

Query: 430 GCLMLMNGGDEAELAGGPGATLGNYQQQGFEVVYDLEKNRVGFARRQCS 471
           G L +                +GN QQQGF VVYDL  +RVGFA   C+
Sbjct: 495 GGLSI----------------IGNIQQQGFRVVYDLASSRVGFAPGGCA 485

[Close]

BLAST of CSPI03G02720 vs. ExPASy Swiss-Prot
Match: Q9LS40 (Protein ASPARTIC PROTEASE IN GUARD CELL 1 OS=Arabidopsis thaliana OX=3702 GN=ASPG1 PE=1 SV=1)

HSP 1 Score: 144.8 bits (364), Expect = 2.5e-33
Identity = 124/421 (29.45%), Postives = 173/421 (41.09%), Query Frame = 0


Query: 60  RHRHNHLSLPLSPG-----GDYTLSFNLGSESHKISLYMDTGSDLVWFPCSPFECILCEG 119
           R++   L+ P+  G     G+Y     +G+ + ++ L +DTGSD+ W  C P  C  C  
Sbjct: 141 RYQTEDLTTPVVSGASQGSGEYFSRIGVGTPAKEMYLVLDTGSDVNWIQCEP--CADCYQ 200

Query: 120 KPKIQSPLPKIANNKSVSCSAAACSAAHGGSLSASHLCAISRCPLESIEISECSSFSCPP 179
           +          +  KS++CSA  CS                      +E S C S  C  
Sbjct: 201 QSDPVFNPTSSSTYKSLTCSAPQCSL---------------------LETSACRSNKC-L 260

Query: 180 FYYAYGDGSL-VARLYRDSLSLPTPAPSPPINVRNFTFGCAHTTLG---EPVGVAGFGRG 239
           +  +YGDGS  V  L  D+++           + N   GC H   G      G+ G G G
Sbjct: 261 YQVSYGDGSFTVGELATDTVTFGNSG-----KINNVALGCGHDNEGLFTGAAGLLGLGGG 320

Query: 240 VLSMPSQLATFSPQLGNRFSYCLVSHSFAADRVRRPSPLILGRYYTGETEFIYTSLLENP 299
           VLS+ +Q+   S      FSYCLV          + S L       G  +     LL N 
Sbjct: 321 VLSITNQMKATS------FSYCLVDRDSG-----KSSSLDFNSVQLGGGD-ATAPLLRNK 380

Query: 300 KHPYFYSVGLAGISVGNVRIPAPEFLTKVDEGGSGGVVVDSGTTFTMLPAGLYESVVAEF 359
           K   FY VGL+G SVG  ++  P+ +  VD  GSGGV++D GT  T L    Y S+   F
Sbjct: 381 KIDTFYYVGLSGFSVGGEKVVLPDAIFDVDASGSGGVILDCGTAVTRLQTQAYNSLRDAF 440

Query: 360 ENRTGKVANRARRIEENTGLSPCYYYE--NSVGVPRVVLHFVGEKSNVVLPRKNYFYEFL 419
              T    N  +     +    CY +   ++V VP V  HF G KS + LP KNY     
Sbjct: 441 LKLT---VNLKKGSSSISLFDTCYDFSSLSTVKVPTVAFHFTGGKS-LDLPAKNYLIPVD 500

Query: 420 DGGDGVVGRKRKVGCLMLMNGGDEAELAGGPGATLGNYQQQGFEVVYDLEKNRVGFARRQ 470
           D G           C           +       +GN QQQG  + YDL KN +G +  +
Sbjct: 501 DSG---------TFCFAFAPTSSSLSI-------IGNVQQQGTRITYDLSKNVIGLSGNK 500

[Close]

BLAST of CSPI03G02720 vs. ExPASy Swiss-Prot
Match: Q766C3 (Aspartic proteinase nepenthesin-1 OS=Nepenthes gracilis OX=150966 GN=nep1 PE=1 SV=1)

HSP 1 Score: 144.8 bits (364), Expect = 2.5e-33
Identity = 121/406 (29.80%), Postives = 181/406 (44.58%), Query Frame = 0


Query: 74  GDYTLSFNLGSESHKISLYMDTGSDLVWFPCSPFECILCEGKPKIQSPLPKIANNKSVSC 133
           G+Y ++ ++G+ +   S  MDTGSDL+W  C P  C  C          P      S S 
Sbjct: 93  GEYLMNLSIGTPAQPFSAIMDTGSDLIWTQCQP--CTQC-----FNQSTPIFNPQGSSSF 152

Query: 134 SAAACSAAHGGSLSASHLCAISRCPLESIEISECSSFSCPPFYYAYGDGSLVARLYRDSL 193
           S   CS         S LC       +++    CS+  C  + Y YGDGS      + S+
Sbjct: 153 STLPCS---------SQLC-------QALSSPTCSNNFC-QYTYGYGDGSET----QGSM 212

Query: 194 SLPTPAPSPPINVRNFTFGCAHTT----LGEPVGVAGFGRGVLSMPSQLATFSPQLGNRF 253
              T      +++ N TFGC         G   G+ G GRG LS+PSQL         +F
Sbjct: 213 GTET-LTFGSVSIPNITFGCGENNQGFGQGNGAGLVGMGRGPLSLPSQLDV------TKF 272

Query: 254 SYCLVSHSFAADRVRRPSPLILGRYYTGETE-FIYTSLLENPKHPYFYSVGLAGISVGNV 313
           SYC+     +      PS L+LG      T     T+L+++ + P FY + L G+SVG+ 
Sbjct: 273 SYCMTPIGSST-----PSNLLLGSLANSVTAGSPNTTLIQSSQIPTFYYITLNGLSVGST 332

Query: 314 RIPA-PEFLTKVDEGGSGGVVVDSGTTFTMLPAGLYESVVAEFENRTG-KVANRARRIEE 373
           R+P  P         G+GG+++DSGTT T      Y+SV  EF ++    V N +     
Sbjct: 333 RLPIDPSAFALNSNNGTGGIIIDSGTTLTYFVNNAYQSVRQEFISQINLPVVNGS----- 392

Query: 374 NTGLSPCYYY---ENSVGVPRVVLHFVGEKSNVVLPRKNYFYEFLDGGDGVVGRKRKVGC 433
           ++G   C+      +++ +P  V+HF G   ++ LP +NY   F+   +G++       C
Sbjct: 393 SSGFDLCFQTPSDPSNLQIPTFVMHFDG--GDLELPSENY---FISPSNGLI-------C 434

Query: 434 LMLMNGGDEAELAGGPGATLGNYQQQGFEVVYDLEKNRVGFARRQC 470
           L + +      +        GN QQQ   VVYD   + V FA  QC
Sbjct: 453 LAMGSSSQGMSI-------FGNIQQQNMLVVYDTGNSVVSFASAQC 434

[Close]

BLAST of CSPI03G02720 vs. ExPASy Swiss-Prot
Match: Q766C2 (Aspartic proteinase nepenthesin-2 OS=Nepenthes gracilis OX=150966 GN=nep2 PE=1 SV=1)

HSP 1 Score: 144.8 bits (364), Expect = 2.5e-33
Identity = 119/405 (29.38%), Postives = 179/405 (44.20%), Query Frame = 0


Query: 74  GDYTLSFNLGSESHKISLYMDTGSDLVWFPCSPFECILCEGKPKIQSPLPKIANNKSVSC 133
           G+Y ++  +G+     S  MDTGSDL+W  C P  C  C        P P      S S 
Sbjct: 94  GEYLMNVAIGTPDSSFSAIMDTGSDLIWTQCEP--CTQC-----FSQPTPIFNPQDSSSF 153

Query: 134 SAAACSAAHGGSLSASHLCAISRCPLESIEISECSSFSCPPFYYAYGDGSLV-ARLYRDS 193
           S   C + +   L           P E+   +EC       + Y YGDGS     +  ++
Sbjct: 154 STLPCESQYCQDL-----------PSETCNNNECQ------YTYGYGDGSTTQGYMATET 213

Query: 194 LSLPTPAPSPPINVRNFTFGCAHTT----LGEPVGVAGFGRGVLSMPSQLATFSPQLGNR 253
            +  T       +V N  FGC         G   G+ G G G LS+PSQL         +
Sbjct: 214 FTFETS------SVPNIAFGCGEDNQGFGQGNGAGLIGMGWGPLSLPSQLGV------GQ 273

Query: 254 FSYCLVSHSFAADRVRRPSPLILGRYYTGETE-FIYTSLLENPKHPYFYSVGLAGISVGN 313
           FSYC+ S+  ++     PS L LG   +G  E    T+L+ +  +P +Y + L GI+VG 
Sbjct: 274 FSYCMTSYGSSS-----PSTLALGSAASGVPEGSPSTTLIHSSLNPTYYYITLQGITVGG 333

Query: 314 VRIPAPEFLTKVDEGGSGGVVVDSGTTFTMLPAGLYESVVAEFENRTGKVANRARRIEEN 373
             +  P    ++ + G+GG+++DSGTT T LP   Y +V   F ++     N     E +
Sbjct: 334 DNLGIPSSTFQLQDDGTGGMIIDSGTTLTYLPQDAYNAVAQAFTDQ----INLPTVDESS 393

Query: 374 TGLSPCYYYE---NSVGVPRVVLHFVGEKSNVVLPRKNYFYEFLDGGDGVVGRKRKVGCL 433
           +GLS C+      ++V VP + + F G   N  L  +N     +   +GV+       CL
Sbjct: 394 SGLSTCFQQPSDGSTVQVPEISMQFDGGVLN--LGEQNI---LISPAEGVI-------CL 435

Query: 434 MLMNGGDEAELAGGPGATLGNYQQQGFEVVYDLEKNRVGFARRQC 470
            +   G  ++L     +  GN QQQ  +V+YDL+   V F   QC
Sbjct: 454 AM---GSSSQLG---ISIFGNIQQQETQVLYDLQNLAVSFVPTQC 435

[Close]

BLAST of CSPI03G02720 vs. ExPASy TrEMBL
Match: A0A0A0L5I7 (Pepsin A OS=Cucumis sativus OX=3659 GN=Csa_3G020060 PE=3 SV=1)

HSP 1 Score: 972.6 bits (2513), Expect = 5.9e-280
Identity = 479/480 (99.79%), Postives = 480/480 (100.00%), Query Frame = 0


Query: 1   MAVSPVFIFLLCFLLSSPVFSSQIFLLPLSHSLSSSISDFNNTHNLLKSTATRSSARFHR 60
           MAVSPVFIFLLCFLLSSPVFSSQIFLLPLSHSLSSSISDFNNTHNLLKSTATRSSARFHR
Sbjct: 1   MAVSPVFIFLLCFLLSSPVFSSQIFLLPLSHSLSSSISDFNNTHNLLKSTATRSSARFHR 60

Query: 61  HRHNHLSLPLSPGGDYTLSFNLGSESHKISLYMDTGSDLVWFPCSPFECILCEGKPKIQS 120
           HRHNHLSLPLSPGGDYTLSFNLGSESHKISLYMDTGSDLVWFPCSPFECILCEGKPKIQS
Sbjct: 61  HRHNHLSLPLSPGGDYTLSFNLGSESHKISLYMDTGSDLVWFPCSPFECILCEGKPKIQS 120

Query: 121 PLPKIANNKSVSCSAAACSAAHGGSLSASHLCAISRCPLESIEISECSSFSCPPFYYAYG 180
           PLPKIANNKSVSCSAAACSAAHGGSLSASHLCAISRCPLESIEISECSSFSCPPFYYAYG
Sbjct: 121 PLPKIANNKSVSCSAAACSAAHGGSLSASHLCAISRCPLESIEISECSSFSCPPFYYAYG 180

Query: 181 DGSLVARLYRDSLSLPTPAPSPPINVRNFTFGCAHTTLGEPVGVAGFGRGVLSMPSQLAT 240
           DGSLVARLYRDSLSLPTPAPSPPINVRNFTFGCAHTTLGEPVGVAGFGRGVLSMPSQLAT
Sbjct: 181 DGSLVARLYRDSLSLPTPAPSPPINVRNFTFGCAHTTLGEPVGVAGFGRGVLSMPSQLAT 240

Query: 241 FSPQLGNRFSYCLVSHSFAADRVRRPSPLILGRYYTGETEFIYTSLLENPKHPYFYSVGL 300
           FSPQLGNRFSYCLVSHSFAADRVRRPSPLILGRYYTGETEFIYTSLLENPKHPYFYSVGL
Sbjct: 241 FSPQLGNRFSYCLVSHSFAADRVRRPSPLILGRYYTGETEFIYTSLLENPKHPYFYSVGL 300

Query: 301 AGISVGNVRIPAPEFLTKVDEGGSGGVVVDSGTTFTMLPAGLYESVVAEFENRTGKVANR 360
           AGISVGN+RIPAPEFLTKVDEGGSGGVVVDSGTTFTMLPAGLYESVVAEFENRTGKVANR
Sbjct: 301 AGISVGNIRIPAPEFLTKVDEGGSGGVVVDSGTTFTMLPAGLYESVVAEFENRTGKVANR 360

Query: 361 ARRIEENTGLSPCYYYENSVGVPRVVLHFVGEKSNVVLPRKNYFYEFLDGGDGVVGRKRK 420
           ARRIEENTGLSPCYYYENSVGVPRVVLHFVGEKSNVVLPRKNYFYEFLDGGDGVVGRKRK
Sbjct: 361 ARRIEENTGLSPCYYYENSVGVPRVVLHFVGEKSNVVLPRKNYFYEFLDGGDGVVGRKRK 420

Query: 421 VGCLMLMNGGDEAELAGGPGATLGNYQQQGFEVVYDLEKNRVGFARRQCSTLWDNLNRSK 480
           VGCLMLMNGGDEAELAGGPGATLGNYQQQGFEVVYDLEKNRVGFARRQCSTLWDNLNRSK
Sbjct: 421 VGCLMLMNGGDEAELAGGPGATLGNYQQQGFEVVYDLEKNRVGFARRQCSTLWDNLNRSK 480

[Close]

BLAST of CSPI03G02720 vs. ExPASy TrEMBL
Match: A0A1S3BK28 (aspartic proteinase nepenthesin-1 OS=Cucumis melo OX=3656 GN=LOC103490888 PE=3 SV=1)

HSP 1 Score: 953.4 bits (2463), Expect = 3.7e-274
Identity = 472/481 (98.13%), Postives = 477/481 (99.17%), Query Frame = 0


Query: 1   MAVSPVFIFLLCFLLSSPVFSSQIFLLPLSHSLSSSISDFNNTHNLLKSTATRSSARFHR 60
           MAVSPVFIFLLCFLLSSPVFSSQIFLLPLSHSLSSSISDFN+THNLLKSTATRSSARFHR
Sbjct: 1   MAVSPVFIFLLCFLLSSPVFSSQIFLLPLSHSLSSSISDFNSTHNLLKSTATRSSARFHR 60

Query: 61  HRHNHLSLPLSPGGDYTLSFNLGSESHKISLYMDTGSDLVWFPCSPFECILCEGKPKIQS 120
           HRHNHLSLPLSPGGDYTLSFNLGSESHKISLYMDTGSDLVWFPCSPFECILCEGKPKIQS
Sbjct: 61  HRHNHLSLPLSPGGDYTLSFNLGSESHKISLYMDTGSDLVWFPCSPFECILCEGKPKIQS 120

Query: 121 PLPKIANNKSVSCSAAACSAAHGGSLSASHLCAISRCPLESIEISECSSFSCPPFYYAYG 180
           PLPKI+NNKSVSCSAAACSAAHGGSLSASHLCAISRCPLESIEISECSSFSCPPFYYAYG
Sbjct: 121 PLPKISNNKSVSCSAAACSAAHGGSLSASHLCAISRCPLESIEISECSSFSCPPFYYAYG 180

Query: 181 DGSLVARLYRDSLSLPTPAPSPPINVRNFTFGCAHTTLGEPVGVAGFGRGVLSMPSQLAT 240
           DGSLVARLYRDSLSLPTPAPSPPINVRNFTFGCAHTTLGEPVGVAGFGRGVLSMPSQLAT
Sbjct: 181 DGSLVARLYRDSLSLPTPAPSPPINVRNFTFGCAHTTLGEPVGVAGFGRGVLSMPSQLAT 240

Query: 241 FSPQLGNRFSYCLVSHSFAADRVRRPSPLILGRYYTGETEFIYTSLLENPKHPYFYSVGL 300
           FSPQLGNRFSYCLVSHSFAADRVRRPSPLILGRY+TGETEFIYTSLLENPKHPYFYSVGL
Sbjct: 241 FSPQLGNRFSYCLVSHSFAADRVRRPSPLILGRYHTGETEFIYTSLLENPKHPYFYSVGL 300

Query: 301 AGISVGNVRIPAPEFLTKVDEGGSGGVVVDSGTTFTMLPAGLYESVVAEFENRTGKVANR 360
           AGISVGNVRIPAPEFL KVDE GSGGVVVDSGTTFTMLP+GLYESVVAEFENRTGKVANR
Sbjct: 301 AGISVGNVRIPAPEFLRKVDESGSGGVVVDSGTTFTMLPSGLYESVVAEFENRTGKVANR 360

Query: 361 ARRIEENTGLSPCYYYENSVGVPRVVLHFVGEKSNVVLPRKNYFYEFLDGGDGV--VGRK 420
           ARRIEENTGLSPCYYYENSVGVPRVVLHFVGEKS+VVLPRKNYFYEFLDGGDGV  VGRK
Sbjct: 361 ARRIEENTGLSPCYYYENSVGVPRVVLHFVGEKSSVVLPRKNYFYEFLDGGDGVVEVGRK 420

Query: 421 RKVGCLMLMNGGDEAELAGGPGATLGNYQQQGFEVVYDLEKNRVGFARRQCSTLWDNLNR 480
           RKVGCLMLMNGGDEAELAGGPGATLGNYQQQGFEVVYDLEKNRVGFARRQCSTLWDNLNR
Sbjct: 421 RKVGCLMLMNGGDEAELAGGPGATLGNYQQQGFEVVYDLEKNRVGFARRQCSTLWDNLNR 480

[Close]

BLAST of CSPI03G02720 vs. ExPASy TrEMBL
Match: A0A5D3CP11 (Aspartic proteinase nepenthesin-1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold1017G00280 PE=3 SV=1)

HSP 1 Score: 947.2 bits (2447), Expect = 2.7e-272
Identity = 471/483 (97.52%), Postives = 477/483 (98.76%), Query Frame = 0


Query: 1   MAVSPVFIFLLCFLLSSPVFSSQIFLLPLSHSLSSSISDFNNTHNLLKSTATRSSARFHR 60
           MAVSPVFIFLLCFLLSSPVFSSQIFLLPLSHSLSSSISDFN+THNLLKSTATRSSARFHR
Sbjct: 1   MAVSPVFIFLLCFLLSSPVFSSQIFLLPLSHSLSSSISDFNSTHNLLKSTATRSSARFHR 60

Query: 61  HRHNHLSLPLSPGGDYTLSFNLGSESHKISLYMDTGSDLVWFPCSPFECILCEGKPKIQS 120
           HRHNHLSLPLSPGGDYTLSFNLGSESHKISLYMDTGSDLVWFPCSPFECILCEGKPKIQS
Sbjct: 61  HRHNHLSLPLSPGGDYTLSFNLGSESHKISLYMDTGSDLVWFPCSPFECILCEGKPKIQS 120

Query: 121 PLPKIANNKSVSCSAAACSAAHGGSLSASHLCAISRCPLESIEISECSSFSCPPFYYAYG 180
           PLPKI+NNKSVSCSAAACSAAHGGSLSASHLCAISRCPLESIEISECSSFSCPPFYYAYG
Sbjct: 121 PLPKISNNKSVSCSAAACSAAHGGSLSASHLCAISRCPLESIEISECSSFSCPPFYYAYG 180

Query: 181 DGSLVARLYRDSLSLPT--PAPSPPINVRNFTFGCAHTTLGEPVGVAGFGRGVLSMPSQL 240
           DGSLVARLYRDSLSLPT  PAPSPPINVRNFTFGCAHTTLGEPVGVAGFGRGVLSMPSQL
Sbjct: 181 DGSLVARLYRDSLSLPTPAPAPSPPINVRNFTFGCAHTTLGEPVGVAGFGRGVLSMPSQL 240

Query: 241 ATFSPQLGNRFSYCLVSHSFAADRVRRPSPLILGRYYTGETEFIYTSLLENPKHPYFYSV 300
           ATFSPQLGNRFSYCLVSHSFAADRVRRPSPLILGRY+TGETEFIYTSLLENPKHPYFYSV
Sbjct: 241 ATFSPQLGNRFSYCLVSHSFAADRVRRPSPLILGRYHTGETEFIYTSLLENPKHPYFYSV 300

Query: 301 GLAGISVGNVRIPAPEFLTKVDEGGSGGVVVDSGTTFTMLPAGLYESVVAEFENRTGKVA 360
           GLAGISVGNVRIPAPEFL KVDE GSGGVVVDSGTTFTMLP+GLYESVVAEFENRTGKVA
Sbjct: 301 GLAGISVGNVRIPAPEFLRKVDESGSGGVVVDSGTTFTMLPSGLYESVVAEFENRTGKVA 360

Query: 361 NRARRIEENTGLSPCYYYENSVGVPRVVLHFVGEKSNVVLPRKNYFYEFLDGGDGV--VG 420
           NRARRIEENTGLSPCYYY+NSVGVPRVVLHFVGEKS+VVLPRKNYFYEFLDGGDGV  VG
Sbjct: 361 NRARRIEENTGLSPCYYYQNSVGVPRVVLHFVGEKSSVVLPRKNYFYEFLDGGDGVVEVG 420

Query: 421 RKRKVGCLMLMNGGDEAELAGGPGATLGNYQQQGFEVVYDLEKNRVGFARRQCSTLWDNL 480
           RKRKVGCLMLMNGGDEAELAGGPGATLGNYQQQGFEVVYDLEKNRVGFARRQCSTLWDNL
Sbjct: 421 RKRKVGCLMLMNGGDEAELAGGPGATLGNYQQQGFEVVYDLEKNRVGFARRQCSTLWDNL 480

[Close]

BLAST of CSPI03G02720 vs. ExPASy TrEMBL
Match: A0A6J1L3Z9 (probable aspartyl protease At4g16563 OS=Cucurbita maxima OX=3661 GN=LOC111500303 PE=3 SV=1)

HSP 1 Score: 869.4 bits (2245), Expect = 7.1e-249
Identity = 432/480 (90.00%), Postives = 450/480 (93.75%), Query Frame = 0


Query: 4   SPVFIFLLCFLLSSPVFSSQIFLLPLSHSLSSSISDFNNTHNLLKSTATRSSARFHR--- 63
           SPVF+FLLCFLL SPVFSSQI LLPLS+SLSSS SDFNNTHNLLKSTA RSSARFH    
Sbjct: 3   SPVFLFLLCFLLPSPVFSSQILLLPLSNSLSSS-SDFNNTHNLLKSTAARSSARFHHRRR 62

Query: 64  -HRHNHLSLPLSPGGDYTLSFNLGSESHKISLYMDTGSDLVWFPCSPFECILCEGKPKIQ 123
            H  +HLSLPLSPGGDYTLSFNLGSES KISLYMDTGSDLVWFPCSPFECILCEGKPKIQ
Sbjct: 63  THHRSHLSLPLSPGGDYTLSFNLGSESQKISLYMDTGSDLVWFPCSPFECILCEGKPKIQ 122

Query: 124 SPLPKIANNKSVSCSAAACSAAHGGSLSASHLCAISRCPLESIEISECSSFSCPPFYYAY 183
           SPLPKI+N KSVSCSAAACSAAHGGSLSASHLCAISRCPLESIE+SECSSFSCPPFYYAY
Sbjct: 123 SPLPKISNQKSVSCSAAACSAAHGGSLSASHLCAISRCPLESIEVSECSSFSCPPFYYAY 182

Query: 184 GDGSLVARLYRDSLSLPTPAPSPPINVRNFTFGCAHTTLGEPVGVAGFGRGVLSMPSQLA 243
           GDGSL+ RLYRDSLSLP PAPSP INVRNFTFGCAH+ LGEP+GVAGFGRG+LSMP QLA
Sbjct: 183 GDGSLIGRLYRDSLSLPAPAPSPAINVRNFTFGCAHSALGEPIGVAGFGRGLLSMPIQLA 242

Query: 244 TFSPQLGNRFSYCLVSHSFAADRVRRPSPLILGRYYTGETEFIYTSLLENPKHPYFYSVG 303
           TFSPQLGNRFSYCLVSHSFAADRVRRPSPLILGRYY  ETEFIYTS+LENPKHPYFYSVG
Sbjct: 243 TFSPQLGNRFSYCLVSHSFAADRVRRPSPLILGRYYGSETEFIYTSMLENPKHPYFYSVG 302

Query: 304 LAGISVGNVRIPAPEFLTKVDEGGSGGVVVDSGTTFTMLPAGLYESVVAEFENRTGKVAN 363
           LAGISVG+V IPAPEFL KVDEGGSGGVVVDSGTTFTMLPAGLY SVVA+FENRTG+VA+
Sbjct: 303 LAGISVGSVMIPAPEFLKKVDEGGSGGVVVDSGTTFTMLPAGLYNSVVAQFENRTGRVAS 362

Query: 364 RARRIEENTGLSPCYYYENSVGVPRVVLHFVGEKSNVVLPRKNYFYEFLDGGDGVVGRKR 423
           RA +IEENTGLSPCYYYE SV VPRVVLHFVGEKS+V+LPRKNYFYEFLDGGDG VGRK 
Sbjct: 363 RASQIEENTGLSPCYYYEKSVEVPRVVLHFVGEKSSVMLPRKNYFYEFLDGGDG-VGRKI 422

Query: 424 KVGCLMLMNGGDEAELAGGPGATLGNYQQQGFEVVYDLEKNRVGFARRQCSTLWDNLNRS 480
           KVGCLMLMNGGDEAELAGGPGATLGNYQQQGFEV YDLE NRVGFARRQCSTLWD+LNRS
Sbjct: 423 KVGCLMLMNGGDEAELAGGPGATLGNYQQQGFEVAYDLENNRVGFARRQCSTLWDSLNRS 480

[Close]

BLAST of CSPI03G02720 vs. ExPASy TrEMBL
Match: A0A6J1EC44 (probable aspartyl protease At4g16563 OS=Cucurbita moschata OX=3662 GN=LOC111431201 PE=3 SV=1)

HSP 1 Score: 867.5 bits (2240), Expect = 2.7e-248
Identity = 433/482 (89.83%), Postives = 451/482 (93.57%), Query Frame = 0


Query: 4   SPVFIFLLCFLLSSPVFSSQIFLLPLSHSLSSSISDFNNTHNLLKSTATRSSARFHR--- 63
           SPVF+FLLCFL SSPVFSSQ+ LLPLS+SLSSS SDFNNTHNLLKSTA RSSARFH    
Sbjct: 3   SPVFLFLLCFLFSSPVFSSQLLLLPLSNSLSSS-SDFNNTHNLLKSTAARSSARFHHRRR 62

Query: 64  -HRHNHLSLPLSPGGDYTLSFNLGSESHKISLYMDTGSDLVWFPCSPFECILCEGKPKIQ 123
            H  +HLSLPLSPGGDYTLSFNLGSES KISLYMDTGSDLVWFPCSPFECILCEGKPKIQ
Sbjct: 63  THHRSHLSLPLSPGGDYTLSFNLGSESQKISLYMDTGSDLVWFPCSPFECILCEGKPKIQ 122

Query: 124 SPLPKIANNKSVSCSAAACSAAHGGSLSASHLCAISRCPLESIEISECSSFSCPPFYYAY 183
           SPLPKI+N KSVSCSAAACSAAHGGSLSASHLCAISRCPLESIE+SECSSFSCPPFYYAY
Sbjct: 123 SPLPKISNQKSVSCSAAACSAAHGGSLSASHLCAISRCPLESIEVSECSSFSCPPFYYAY 182

Query: 184 GDGSLVARLYRDSLSL--PTPAPSPPINVRNFTFGCAHTTLGEPVGVAGFGRGVLSMPSQ 243
           GDGSL+ RLYRDSLSL  P PAPSP INVRNFTFGCAH+ LGEP+GVAGFGRG+LSMPSQ
Sbjct: 183 GDGSLIGRLYRDSLSLPAPAPAPSPAINVRNFTFGCAHSALGEPIGVAGFGRGLLSMPSQ 242

Query: 244 LATFSPQLGNRFSYCLVSHSFAADRVRRPSPLILGRYYTGETEFIYTSLLENPKHPYFYS 303
           LATFSPQLGNRFSYCLVSHSFAADRVRRPSPLILGRYY  ETEFIYTS+LENPKHPYFYS
Sbjct: 243 LATFSPQLGNRFSYCLVSHSFAADRVRRPSPLILGRYYGSETEFIYTSMLENPKHPYFYS 302

Query: 304 VGLAGISVGNVRIPAPEFLTKVDEGGSGGVVVDSGTTFTMLPAGLYESVVAEFENRTGKV 363
           VGLAGISVG+VRIPAPEFL +VDEGGSGGVVVDSGTTFTMLPAGLY SVVA+FENRTG+V
Sbjct: 303 VGLAGISVGSVRIPAPEFLKRVDEGGSGGVVVDSGTTFTMLPAGLYNSVVAQFENRTGRV 362

Query: 364 ANRARRIEENTGLSPCYYYENSVGVPRVVLHFVGEKSNVVLPRKNYFYEFLDGGDGVVGR 423
           A+RA RIEENTGLSPCY YE SV VPRVVLHFVGEKS+V LPRKNYFYEFLDGGDG VGR
Sbjct: 363 ASRASRIEENTGLSPCYSYEKSVEVPRVVLHFVGEKSSVELPRKNYFYEFLDGGDG-VGR 422

Query: 424 KRKVGCLMLMNGGDEAELAGGPGATLGNYQQQGFEVVYDLEKNRVGFARRQCSTLWDNLN 480
           KRKVGCLMLMNGGDEAELAGGPGATLGNYQQQGFEV YDLE NRVGFARRQCSTLWD+LN
Sbjct: 423 KRKVGCLMLMNGGDEAELAGGPGATLGNYQQQGFEVAYDLENNRVGFARRQCSTLWDSLN 482

[Close]

BLAST of CSPI03G02720 vs. NCBI nr
Match: XP_004147205.1 (probable aspartyl protease At4g16563 [Cucumis sativus])

HSP 1 Score: 972.6 bits (2513), Expect = 1.2e-279
Identity = 479/480 (99.79%), Postives = 480/480 (100.00%), Query Frame = 0


Query: 1   MAVSPVFIFLLCFLLSSPVFSSQIFLLPLSHSLSSSISDFNNTHNLLKSTATRSSARFHR 60
           MAVSPVFIFLLCFLLSSPVFSSQIFLLPLSHSLSSSISDFNNTHNLLKSTATRSSARFHR
Sbjct: 1   MAVSPVFIFLLCFLLSSPVFSSQIFLLPLSHSLSSSISDFNNTHNLLKSTATRSSARFHR 60

Query: 61  HRHNHLSLPLSPGGDYTLSFNLGSESHKISLYMDTGSDLVWFPCSPFECILCEGKPKIQS 120
           HRHNHLSLPLSPGGDYTLSFNLGSESHKISLYMDTGSDLVWFPCSPFECILCEGKPKIQS
Sbjct: 61  HRHNHLSLPLSPGGDYTLSFNLGSESHKISLYMDTGSDLVWFPCSPFECILCEGKPKIQS 120

Query: 121 PLPKIANNKSVSCSAAACSAAHGGSLSASHLCAISRCPLESIEISECSSFSCPPFYYAYG 180
           PLPKIANNKSVSCSAAACSAAHGGSLSASHLCAISRCPLESIEISECSSFSCPPFYYAYG
Sbjct: 121 PLPKIANNKSVSCSAAACSAAHGGSLSASHLCAISRCPLESIEISECSSFSCPPFYYAYG 180

Query: 181 DGSLVARLYRDSLSLPTPAPSPPINVRNFTFGCAHTTLGEPVGVAGFGRGVLSMPSQLAT 240
           DGSLVARLYRDSLSLPTPAPSPPINVRNFTFGCAHTTLGEPVGVAGFGRGVLSMPSQLAT
Sbjct: 181 DGSLVARLYRDSLSLPTPAPSPPINVRNFTFGCAHTTLGEPVGVAGFGRGVLSMPSQLAT 240

Query: 241 FSPQLGNRFSYCLVSHSFAADRVRRPSPLILGRYYTGETEFIYTSLLENPKHPYFYSVGL 300
           FSPQLGNRFSYCLVSHSFAADRVRRPSPLILGRYYTGETEFIYTSLLENPKHPYFYSVGL
Sbjct: 241 FSPQLGNRFSYCLVSHSFAADRVRRPSPLILGRYYTGETEFIYTSLLENPKHPYFYSVGL 300

Query: 301 AGISVGNVRIPAPEFLTKVDEGGSGGVVVDSGTTFTMLPAGLYESVVAEFENRTGKVANR 360
           AGISVGN+RIPAPEFLTKVDEGGSGGVVVDSGTTFTMLPAGLYESVVAEFENRTGKVANR
Sbjct: 301 AGISVGNIRIPAPEFLTKVDEGGSGGVVVDSGTTFTMLPAGLYESVVAEFENRTGKVANR 360

Query: 361 ARRIEENTGLSPCYYYENSVGVPRVVLHFVGEKSNVVLPRKNYFYEFLDGGDGVVGRKRK 420
           ARRIEENTGLSPCYYYENSVGVPRVVLHFVGEKSNVVLPRKNYFYEFLDGGDGVVGRKRK
Sbjct: 361 ARRIEENTGLSPCYYYENSVGVPRVVLHFVGEKSNVVLPRKNYFYEFLDGGDGVVGRKRK 420

Query: 421 VGCLMLMNGGDEAELAGGPGATLGNYQQQGFEVVYDLEKNRVGFARRQCSTLWDNLNRSK 480
           VGCLMLMNGGDEAELAGGPGATLGNYQQQGFEVVYDLEKNRVGFARRQCSTLWDNLNRSK
Sbjct: 421 VGCLMLMNGGDEAELAGGPGATLGNYQQQGFEVVYDLEKNRVGFARRQCSTLWDNLNRSK 480

[Close]

BLAST of CSPI03G02720 vs. NCBI nr
Match: XP_008448851.1 (PREDICTED: aspartic proteinase nepenthesin-1 [Cucumis melo])

HSP 1 Score: 953.4 bits (2463), Expect = 7.7e-274
Identity = 472/481 (98.13%), Postives = 477/481 (99.17%), Query Frame = 0


Query: 1   MAVSPVFIFLLCFLLSSPVFSSQIFLLPLSHSLSSSISDFNNTHNLLKSTATRSSARFHR 60
           MAVSPVFIFLLCFLLSSPVFSSQIFLLPLSHSLSSSISDFN+THNLLKSTATRSSARFHR
Sbjct: 1   MAVSPVFIFLLCFLLSSPVFSSQIFLLPLSHSLSSSISDFNSTHNLLKSTATRSSARFHR 60

Query: 61  HRHNHLSLPLSPGGDYTLSFNLGSESHKISLYMDTGSDLVWFPCSPFECILCEGKPKIQS 120
           HRHNHLSLPLSPGGDYTLSFNLGSESHKISLYMDTGSDLVWFPCSPFECILCEGKPKIQS
Sbjct: 61  HRHNHLSLPLSPGGDYTLSFNLGSESHKISLYMDTGSDLVWFPCSPFECILCEGKPKIQS 120

Query: 121 PLPKIANNKSVSCSAAACSAAHGGSLSASHLCAISRCPLESIEISECSSFSCPPFYYAYG 180
           PLPKI+NNKSVSCSAAACSAAHGGSLSASHLCAISRCPLESIEISECSSFSCPPFYYAYG
Sbjct: 121 PLPKISNNKSVSCSAAACSAAHGGSLSASHLCAISRCPLESIEISECSSFSCPPFYYAYG 180

Query: 181 DGSLVARLYRDSLSLPTPAPSPPINVRNFTFGCAHTTLGEPVGVAGFGRGVLSMPSQLAT 240
           DGSLVARLYRDSLSLPTPAPSPPINVRNFTFGCAHTTLGEPVGVAGFGRGVLSMPSQLAT
Sbjct: 181 DGSLVARLYRDSLSLPTPAPSPPINVRNFTFGCAHTTLGEPVGVAGFGRGVLSMPSQLAT 240

Query: 241 FSPQLGNRFSYCLVSHSFAADRVRRPSPLILGRYYTGETEFIYTSLLENPKHPYFYSVGL 300
           FSPQLGNRFSYCLVSHSFAADRVRRPSPLILGRY+TGETEFIYTSLLENPKHPYFYSVGL
Sbjct: 241 FSPQLGNRFSYCLVSHSFAADRVRRPSPLILGRYHTGETEFIYTSLLENPKHPYFYSVGL 300

Query: 301 AGISVGNVRIPAPEFLTKVDEGGSGGVVVDSGTTFTMLPAGLYESVVAEFENRTGKVANR 360
           AGISVGNVRIPAPEFL KVDE GSGGVVVDSGTTFTMLP+GLYESVVAEFENRTGKVANR
Sbjct: 301 AGISVGNVRIPAPEFLRKVDESGSGGVVVDSGTTFTMLPSGLYESVVAEFENRTGKVANR 360

Query: 361 ARRIEENTGLSPCYYYENSVGVPRVVLHFVGEKSNVVLPRKNYFYEFLDGGDGV--VGRK 420
           ARRIEENTGLSPCYYYENSVGVPRVVLHFVGEKS+VVLPRKNYFYEFLDGGDGV  VGRK
Sbjct: 361 ARRIEENTGLSPCYYYENSVGVPRVVLHFVGEKSSVVLPRKNYFYEFLDGGDGVVEVGRK 420

Query: 421 RKVGCLMLMNGGDEAELAGGPGATLGNYQQQGFEVVYDLEKNRVGFARRQCSTLWDNLNR 480
           RKVGCLMLMNGGDEAELAGGPGATLGNYQQQGFEVVYDLEKNRVGFARRQCSTLWDNLNR
Sbjct: 421 RKVGCLMLMNGGDEAELAGGPGATLGNYQQQGFEVVYDLEKNRVGFARRQCSTLWDNLNR 480

[Close]

BLAST of CSPI03G02720 vs. NCBI nr
Match: TYK12019.1 (aspartic proteinase nepenthesin-1 [Cucumis melo var. makuwa])

HSP 1 Score: 947.2 bits (2447), Expect = 5.5e-272
Identity = 471/483 (97.52%), Postives = 477/483 (98.76%), Query Frame = 0


Query: 1   MAVSPVFIFLLCFLLSSPVFSSQIFLLPLSHSLSSSISDFNNTHNLLKSTATRSSARFHR 60
           MAVSPVFIFLLCFLLSSPVFSSQIFLLPLSHSLSSSISDFN+THNLLKSTATRSSARFHR
Sbjct: 1   MAVSPVFIFLLCFLLSSPVFSSQIFLLPLSHSLSSSISDFNSTHNLLKSTATRSSARFHR 60

Query: 61  HRHNHLSLPLSPGGDYTLSFNLGSESHKISLYMDTGSDLVWFPCSPFECILCEGKPKIQS 120
           HRHNHLSLPLSPGGDYTLSFNLGSESHKISLYMDTGSDLVWFPCSPFECILCEGKPKIQS
Sbjct: 61  HRHNHLSLPLSPGGDYTLSFNLGSESHKISLYMDTGSDLVWFPCSPFECILCEGKPKIQS 120

Query: 121 PLPKIANNKSVSCSAAACSAAHGGSLSASHLCAISRCPLESIEISECSSFSCPPFYYAYG 180
           PLPKI+NNKSVSCSAAACSAAHGGSLSASHLCAISRCPLESIEISECSSFSCPPFYYAYG
Sbjct: 121 PLPKISNNKSVSCSAAACSAAHGGSLSASHLCAISRCPLESIEISECSSFSCPPFYYAYG 180

Query: 181 DGSLVARLYRDSLSLPT--PAPSPPINVRNFTFGCAHTTLGEPVGVAGFGRGVLSMPSQL 240
           DGSLVARLYRDSLSLPT  PAPSPPINVRNFTFGCAHTTLGEPVGVAGFGRGVLSMPSQL
Sbjct: 181 DGSLVARLYRDSLSLPTPAPAPSPPINVRNFTFGCAHTTLGEPVGVAGFGRGVLSMPSQL 240

Query: 241 ATFSPQLGNRFSYCLVSHSFAADRVRRPSPLILGRYYTGETEFIYTSLLENPKHPYFYSV 300
           ATFSPQLGNRFSYCLVSHSFAADRVRRPSPLILGRY+TGETEFIYTSLLENPKHPYFYSV
Sbjct: 241 ATFSPQLGNRFSYCLVSHSFAADRVRRPSPLILGRYHTGETEFIYTSLLENPKHPYFYSV 300

Query: 301 GLAGISVGNVRIPAPEFLTKVDEGGSGGVVVDSGTTFTMLPAGLYESVVAEFENRTGKVA 360
           GLAGISVGNVRIPAPEFL KVDE GSGGVVVDSGTTFTMLP+GLYESVVAEFENRTGKVA
Sbjct: 301 GLAGISVGNVRIPAPEFLRKVDESGSGGVVVDSGTTFTMLPSGLYESVVAEFENRTGKVA 360

Query: 361 NRARRIEENTGLSPCYYYENSVGVPRVVLHFVGEKSNVVLPRKNYFYEFLDGGDGV--VG 420
           NRARRIEENTGLSPCYYY+NSVGVPRVVLHFVGEKS+VVLPRKNYFYEFLDGGDGV  VG
Sbjct: 361 NRARRIEENTGLSPCYYYQNSVGVPRVVLHFVGEKSSVVLPRKNYFYEFLDGGDGVVEVG 420

Query: 421 RKRKVGCLMLMNGGDEAELAGGPGATLGNYQQQGFEVVYDLEKNRVGFARRQCSTLWDNL 480
           RKRKVGCLMLMNGGDEAELAGGPGATLGNYQQQGFEVVYDLEKNRVGFARRQCSTLWDNL
Sbjct: 421 RKRKVGCLMLMNGGDEAELAGGPGATLGNYQQQGFEVVYDLEKNRVGFARRQCSTLWDNL 480

[Close]

BLAST of CSPI03G02720 vs. NCBI nr
Match: XP_038905814.1 (probable aspartyl protease At4g16563 [Benincasa hispida])

HSP 1 Score: 894.4 bits (2310), Expect = 4.2e-256
Identity = 441/480 (91.88%), Postives = 456/480 (95.00%), Query Frame = 0


Query: 4   SPVFIFLLCFLLSSPVFSSQIFLLPLSHSLSSSISDFNNTHNLLKSTATRSSARFHRHR- 63
           S VF+ LLCFLLSSPVFSSQ+ LLPLSHSLSSSISDFNNTHNLLKSTA RSSARFH  R 
Sbjct: 3   SSVFVLLLCFLLSSPVFSSQLLLLPLSHSLSSSISDFNNTHNLLKSTAARSSARFHHRRR 62

Query: 64  ---HNHLSLPLSPGGDYTLSFNLGSESHKISLYMDTGSDLVWFPCSPFECILCEGKPKIQ 123
              HNHLSLPLSPGGDYTLSFNLGSESHKISLYMDTGSDLVWFPCSPFECILCEGKPK+Q
Sbjct: 63  TQHHNHLSLPLSPGGDYTLSFNLGSESHKISLYMDTGSDLVWFPCSPFECILCEGKPKVQ 122

Query: 124 SPLPKIANNKSVSCSAAACSAAHGGSLSASHLCAISRCPLESIEISECSSFSCPPFYYAY 183
           SPLPKI+NNKSVSCSA ACSAAHGGSLSASHLCAIS+CPLESIEISECSSFSCPPFYYAY
Sbjct: 123 SPLPKISNNKSVSCSAPACSAAHGGSLSASHLCAISQCPLESIEISECSSFSCPPFYYAY 182

Query: 184 GDGSLVARLYRDSLSLPTPAPSPPINVRNFTFGCAHTTLGEPVGVAGFGRGVLSMPSQLA 243
           GDGSL+ARLYRDSLSLP PAPSP INVRNFTFGCAHT LGEPVGVAGFGRG LSMPSQLA
Sbjct: 183 GDGSLIARLYRDSLSLPAPAPSPAINVRNFTFGCAHTALGEPVGVAGFGRGTLSMPSQLA 242

Query: 244 TFSPQLGNRFSYCLVSHSFAADRVRRPSPLILGRYYTGETEFIYTSLLENPKHPYFYSVG 303
           TFSPQLGNRFSYCLVSHSFAA+RVRRPSPLILGRYY GETEFIYTSLLENPKHPYFYSVG
Sbjct: 243 TFSPQLGNRFSYCLVSHSFAAERVRRPSPLILGRYYGGETEFIYTSLLENPKHPYFYSVG 302

Query: 304 LAGISVGNVRIPAPEFLTKVDEGGSGGVVVDSGTTFTMLPAGLYESVVAEFENRTGKVAN 363
           L GISVGN+ IPAPEFL KVDEGGSGGVVVDSGTTFTMLPAGLY+SVVA FENRTG+VAN
Sbjct: 303 LTGISVGNMMIPAPEFLKKVDEGGSGGVVVDSGTTFTMLPAGLYDSVVAAFENRTGRVAN 362

Query: 364 RARRIEENTGLSPCYYYENSVGVPRVVLHFVGEKSNVVLPRKNYFYEFLDGGDGVVGRKR 423
           RARRIEENTGLSPCYYYENSV VPRVVLHFVGEKS+V+LP+KNYFYEFLDGGDG VG+KR
Sbjct: 363 RARRIEENTGLSPCYYYENSVEVPRVVLHFVGEKSSVLLPKKNYFYEFLDGGDG-VGKKR 422

Query: 424 KVGCLMLMNGGDEAELAGGPGATLGNYQQQGFEVVYDLEKNRVGFARRQCSTLWDNLNRS 480
           KVGCLMLMNGGDEAELAGGPGATLGNYQQQGFEV YDL KNRVGFARRQCSTLWD+LNRS
Sbjct: 423 KVGCLMLMNGGDEAELAGGPGATLGNYQQQGFEVAYDLAKNRVGFARRQCSTLWDSLNRS 481

[Close]

BLAST of CSPI03G02720 vs. NCBI nr
Match: KAE8650060.1 (hypothetical protein Csa_011084 [Cucumis sativus])

HSP 1 Score: 883.6 bits (2282), Expect = 7.5e-253
Identity = 437/438 (99.77%), Postives = 438/438 (100.00%), Query Frame = 0


Query: 1   MAVSPVFIFLLCFLLSSPVFSSQIFLLPLSHSLSSSISDFNNTHNLLKSTATRSSARFHR 60
           MAVSPVFIFLLCFLLSSPVFSSQIFLLPLSHSLSSSISDFNNTHNLLKSTATRSSARFHR
Sbjct: 1   MAVSPVFIFLLCFLLSSPVFSSQIFLLPLSHSLSSSISDFNNTHNLLKSTATRSSARFHR 60

Query: 61  HRHNHLSLPLSPGGDYTLSFNLGSESHKISLYMDTGSDLVWFPCSPFECILCEGKPKIQS 120
           HRHNHLSLPLSPGGDYTLSFNLGSESHKISLYMDTGSDLVWFPCSPFECILCEGKPKIQS
Sbjct: 61  HRHNHLSLPLSPGGDYTLSFNLGSESHKISLYMDTGSDLVWFPCSPFECILCEGKPKIQS 120

Query: 121 PLPKIANNKSVSCSAAACSAAHGGSLSASHLCAISRCPLESIEISECSSFSCPPFYYAYG 180
           PLPKIANNKSVSCSAAACSAAHGGSLSASHLCAISRCPLESIEISECSSFSCPPFYYAYG
Sbjct: 121 PLPKIANNKSVSCSAAACSAAHGGSLSASHLCAISRCPLESIEISECSSFSCPPFYYAYG 180

Query: 181 DGSLVARLYRDSLSLPTPAPSPPINVRNFTFGCAHTTLGEPVGVAGFGRGVLSMPSQLAT 240
           DGSLVARLYRDSLSLPTPAPSPPINVRNFTFGCAHTTLGEPVGVAGFGRGVLSMPSQLAT
Sbjct: 181 DGSLVARLYRDSLSLPTPAPSPPINVRNFTFGCAHTTLGEPVGVAGFGRGVLSMPSQLAT 240

Query: 241 FSPQLGNRFSYCLVSHSFAADRVRRPSPLILGRYYTGETEFIYTSLLENPKHPYFYSVGL 300
           FSPQLGNRFSYCLVSHSFAADRVRRPSPLILGRYYTGETEFIYTSLLENPKHPYFYSVGL
Sbjct: 241 FSPQLGNRFSYCLVSHSFAADRVRRPSPLILGRYYTGETEFIYTSLLENPKHPYFYSVGL 300

Query: 301 AGISVGNVRIPAPEFLTKVDEGGSGGVVVDSGTTFTMLPAGLYESVVAEFENRTGKVANR 360
           AGISVGN+RIPAPEFLTKVDEGGSGGVVVDSGTTFTMLPAGLYESVVAEFENRTGKVANR
Sbjct: 301 AGISVGNIRIPAPEFLTKVDEGGSGGVVVDSGTTFTMLPAGLYESVVAEFENRTGKVANR 360

Query: 361 ARRIEENTGLSPCYYYENSVGVPRVVLHFVGEKSNVVLPRKNYFYEFLDGGDGVVGRKRK 420
           ARRIEENTGLSPCYYYENSVGVPRVVLHFVGEKSNVVLPRKNYFYEFLDGGDGVVGRKRK
Sbjct: 361 ARRIEENTGLSPCYYYENSVGVPRVVLHFVGEKSNVVLPRKNYFYEFLDGGDGVVGRKRK 420

Query: 421 VGCLMLMNGGDEAELAGG 439
           VGCLMLMNGGDEAELAGG
Sbjct: 421 VGCLMLMNGGDEAELAGG 438

[Close]

BLAST of CSPI03G02720 vs. TAIR 10
Match: AT4G16563.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 581.6 bits (1498), Expect = 5.7e-166
Identity = 298/478 (62.34%), Postives = 356/478 (74.48%), Query Frame = 0


Query: 26  LLPLSHSLSSSISDFNNTHNLLKSTATRSSARFHRHRH----NHLSLPLSPGGDYTLSFN 85
           LL LSHSLS+S    +  H LLKS+++RSSARF RH H      LSLP+S G DY +S +
Sbjct: 30  LLHLSHSLSTSKHSSSPLH-LLKSSSSRSSARFRRHHHKQQQQQLSLPISSGSDYLISLS 89

Query: 86  LGSESHKISLYMDTGSDLVWFPCSPFECILCEGKPKIQSPLPKIANN-KSVSCSAAACSA 145
           +GS S  +SLY+DTGSDLVWFPC PF CILCE KP   SP   ++++  +VSCS+ +CSA
Sbjct: 90  VGSSSSAVSLYLDTGSDLVWFPCRPFTCILCESKPLPPSPPSSLSSSATTVSCSSPSCSA 149

Query: 146 AHGGSLSASHLCAISRCPLESIEISEC--SSFSCPPFYYAYGDGSLVARLYRDSLSLPTP 205
           AH  SL +S LCAIS CPL+ IE  +C  SS+ CPPFYYAYGDGSLVA+LY DSLSLP+ 
Sbjct: 150 AH-SSLPSSDLCAISNCPLDFIETGDCNTSSYPCPPFYYAYGDGSLVAKLYSDSLSLPS- 209

Query: 206 APSPPINVRNFTFGCAHTTLGEPVGVAGFGRGVLSMPSQLATFSPQLGNRFSYCLVSHSF 265
                ++V NFTFGCAHTTL EP+GVAGFGRG LS+P+QLA  SP LGN FSYCLVSHSF
Sbjct: 210 -----VSVSNFTFGCAHTTLAEPIGVAGFGRGRLSLPAQLAVHSPHLGNSFSYCLVSHSF 269

Query: 266 AADRVRRPSPLILGRYY------TGET--------------EFIYTSLLENPKHPYFYSV 325
            +DRVRRPSPLILGR+        G T              EF++T +LENPKHPYFYSV
Sbjct: 270 DSDRVRRPSPLILGRFVDKKEKRVGTTDDHDDGDDEKKKKNEFVFTEMLENPKHPYFYSV 329

Query: 326 GLAGISVGNVRIPAPEFLTKVDEGGSGGVVVDSGTTFTMLPAGLYESVVAEFENRTGKVA 385
            L GIS+G   IPAP  L ++D+ G GGVVVDSGTTFTMLPA  Y SVV EF++R G+V 
Sbjct: 330 SLQGISIGKRNIPAPAMLRRIDKNGGGGVVVDSGTTFTMLPAKFYNSVVEEFDSRVGRVH 389

Query: 386 NRARRIEENTGLSPCYYYENSVGVPRVVLHFVGEKSNVVLPRKNYFYEFLDGGDGVVGRK 445
            RA R+E ++G+SPCYY   +V VP +VLHF G +S+V LPR+NYFYEF+DGGDG    K
Sbjct: 390 ERADRVEPSSGMSPCYYLNQTVKVPALVLHFAGNRSSVTLPRRNYFYEFMDGGDG-KEEK 449

Query: 446 RKVGCLMLMNGGDEAELAGGPGATLGNYQQQGFEVVYDLEKNRVGFARRQCSTLWDNL 477
           RK+GCLMLMNGGDE+EL GG GA LGNYQQQGFEVVYDL   RVGFA+R+C++LWD+L
Sbjct: 450 RKIGCLMLMNGGDESELRGGTGAILGNYQQQGFEVVYDLLNRRVGFAKRKCASLWDSL 498

[Close]

BLAST of CSPI03G02720 vs. TAIR 10
Match: AT5G45120.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 208.4 bits (529), Expect = 1.3e-53
Identity = 145/421 (34.44%), Postives = 204/421 (48.46%), Query Frame = 0


Query: 76  YTLSFNLGSESHKISLYMDTGSDLVWFPCS--PFECILCEG-------KPKIQSPLPKIA 135
           Y ++ N+G+    + +Y+DTGSDL W PC    F+CI C          P + SPL    
Sbjct: 83  YLITLNIGTPPQAVQVYLDTGSDLTWVPCGNLSFDCIECYDLKNNDLKSPSVFSPLHSST 142

Query: 136 NNKSVSCSAAACSAAHGGSLSASHLCAISRCPLESIEISECSSFSCPPFYYAYGDGSLVA 195
           + +  SC+++ C   H  S +    CA++ C +  +  S C    CP F Y YG+G L++
Sbjct: 143 SFRD-SCASSFCVEIH-SSDNPFDPCAVAGCSVSMLLKSTCVR-PCPSFAYTYGEGGLIS 202

Query: 196 R-LYRDSLSLPTPAPSPPINVRNFTFGCAHTTLGEPVGVAGFGRGVLSMPSQLATFSPQL 255
             L RD L   T       +V  F+FGC  +T  EP+G+AGFGRG+LS+PSQL      L
Sbjct: 203 GILTRDILKARTR------DVPRFSFGCVTSTYREPIGIAGFGRGLLSLPSQLGF----L 262

Query: 256 GNRFSYCLVSHSFAADRVRRPSPLILGRYYTG---ETEFIYTSLLENPKHPYFYSVGLAG 315
              FS+C +   F  +     SPLILG             +T +L  P +P  Y +GL  
Sbjct: 263 EKGFSHCFLPFKF-VNNPNISSPLILGASALSINLTDSLQFTPMLNTPMYPNSYYIGLES 322

Query: 316 ISVGNVRIP--APEFLTKVDEGGSGGVVVDSGTTFTMLPAGLYESVVAEFENRTGKVANR 375
           I++G    P   P  L + D  G+GG++VDSGTT+T LP   Y  ++   ++       R
Sbjct: 323 ITIGTNITPTQVPLTLRQFDSQGNGGMLVDSGTTYTHLPEPFYSQLLTTLQSTI--TYPR 382

Query: 376 ARRIEENTGLSPCY----------YYENSVGV--PRVVLHFVGEKSNVVLPRKNYFYEFL 435
           A   E  TG   CY            EN V +  P +  HF+   + ++LP+ N FY   
Sbjct: 383 ATETESRTGFDLCYKVPCPNNNLTSLENDVMMIFPSITFHFL-NNATLLLPQGNSFYAMS 442

Query: 436 DGGDGVVGRKRKVGCLMLMNGGDEAELAGGPGATLGNYQQQGFEVVYDLEKNRVGFARRQ 470
              DG V     V CL+  N  D      GP    G++QQQ  +VVYDLEK R+GF    
Sbjct: 443 APSDGSV-----VQCLLFQNMEDGDY---GPAGVFGSFQQQNVKVVYDLEKERIGFQAMD 478

[Close]

BLAST of CSPI03G02720 vs. TAIR 10
Match: AT3G52500.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 178.3 bits (451), Expect = 1.4e-44
Identity = 151/505 (29.90%), Postives = 226/505 (44.75%), Query Frame = 0


Query: 4   SPVFIFLLCFLLSSPVFSSQIFLLPLSHSLSSSISDFNNTHNLLKSTATRSSARFHRHRH 63
           S +F F L FL  S V + ++ L P SHS  S    + +   L +S    S AR H+ +H
Sbjct: 3   SSIFFFFLIFL--SVVSAVKLPLSPFSHSDQSPKDPYLSLRRLAES----SIARAHKLKH 62

Query: 64  NH-------------------LSLPLSPG--GDYTLSFNLGSESHKISLYMDTGSDLVWF 123
                                +  PLS    G Y++S + G+ S  I    DTGS LVW 
Sbjct: 63  GTSIKPDEDALSSTTTASATVVKSPLSAKSYGGYSVSLSFGTPSQTIPFVFDTGSSLVWL 122

Query: 124 PC-SPFECILCEGKPKIQSPLPKI-----ANNKSVSCSAAACSAAHGGSLSASHLCAISR 183
           PC S + C  C+      + +P+      +++K + C +  C   +G ++         +
Sbjct: 123 PCTSRYLCSGCDFSGLDPTLIPRFIPKNSSSSKIIGCQSPKCQFLYGPNV---------Q 182

Query: 184 CPLESIEISECSSFSCPPFYYAYGDGSLVARLYRDSLSLPTPAPSPPINVRNFTFGCAHT 243
           C         C +  CPP+   YG GS    L  + L        P + V +F  GC+  
Sbjct: 183 CRGCDPNTRNC-TVGCPPYILQYGLGSTAGVLITEKLDF------PDLTVPDFVVGCSII 242

Query: 244 TLGEPVGVAGFGRGVLSMPSQLATFSPQLGNRFSYCLVSHSFAADRVRRPSPLILGRYYT 303
           +  +P G+AGFGRG +S+PSQ+         RFS+CLVS  F    V     L  G  + 
Sbjct: 243 STRQPAGIAGFGRGPVSLPSQMNL------KRFSHCLVSRRFDDTNVTTDLDLDTGSGHN 302

Query: 304 GETE---FIYTSLLENPK-----HPYFYSVGLAGISVGNVRIPAPEFLTKVDEGGSGGVV 363
             ++     YT   +NP         +Y + L  I VG   +  P         G GG +
Sbjct: 303 SGSKTPGLTYTPFRKNPNVSNKAFLEYYYLNLRRIYVGRKHVKIPYKYLAPGTNGDGGSI 362

Query: 364 VDSGTTFTMLPAGLYESVVAEFENRTGKVANRARRIEENTGLSPCYYY--ENSVGVPRVV 423
           VDSG+TFT +   ++E V  EF ++      R + +E+ TGL PC+    +  V VP ++
Sbjct: 363 VDSGSTFTFMERPVFELVAEEFASQMSNY-TREKDLEKETGLGPCFNISGKGDVTVPELI 422

Query: 424 LHFVGEKSNVVLPRKNYFYEFLDGGDGVVGRKRKVGCLMLMNGGDEAELAG-GPGATLGN 471
             F G  + + LP  NYF  F+   D V        CL +++        G GP   LG+
Sbjct: 423 FEFKG-GAKLELPLSNYF-TFVGNTDTV--------CLTVVSDKTVNPSGGTGPAIILGS 468

[Close]

BLAST of CSPI03G02720 vs. TAIR 10
Match: AT3G61820.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 152.5 bits (384), Expect = 8.5e-37
Identity = 131/407 (32.19%), Postives = 175/407 (43.00%), Query Frame = 0


Query: 70  LSPG-GDYTLSFNLGSESHKISLYMDTGSDLVWFPCSPFECILCEGKPKIQSPLPKIANN 129
           LS G G+Y +   +G+ +  + + +DTGSD+VW  CSP  C  C  +        K    
Sbjct: 128 LSQGSGEYFMRLGVGTPATNVYMVLDTGSDVVWLQCSP--CKACYNQTDAIFDPKKSKTF 187

Query: 130 KSVSCSAAACSAAHGGSLSASHLCAISRCPLESIEISECSSFSCPPFYYAYGDGSLVARL 189
            +V C +  C       L  S  C   R            S +C  +  +YGDGS     
Sbjct: 188 ATVPCGSRLCR-----RLDDSSECVTRR------------SKTC-LYQVSYGDGSFT--- 247

Query: 190 YRDSLSLPTPAPSPPINVRNFTFGCAHTTLGEPVGVA---GFGRGVLSMPSQLATFSPQL 249
                S  T        V +   GC H   G  VG A   G GRG LS PSQ      + 
Sbjct: 248 -EGDFSTET-LTFHGARVDHVPLGCGHDNEGLFVGAAGLLGLGRGGLSFPSQTKN---RY 307

Query: 250 GNRFSYCLVSHSFAADRVRRPSPLILGRYYTGETEFIYTSLLENPKHPYFYSVGLAGISV 309
             +FSYCLV  + +    + PS ++ G     +T  ++T LL NPK   FY + L GISV
Sbjct: 308 NGKFSYCLVDRTSSGSSSKPPSTIVFGNAAVPKTS-VFTPLLTNPKLDTFYYLQLLGISV 367

Query: 310 GNVRIP-APEFLTKVDEGGSGGVVVDSGTTFTMLPAGLYESVVAEFENRTGKVANRARRI 369
           G  R+P   E   K+D  G+GGV++DSGT+ T L    Y ++   F  R G  A + +R 
Sbjct: 368 GGSRVPGVSESQFKLDATGNGGVIIDSGTSVTRLTQPAYVALRDAF--RLG--ATKLKRA 427

Query: 370 EENTGLSPCYYYE--NSVGVPRVVLHFVGEKSNVVLPRKNYFYEFLDGGDGVVGRKRKVG 429
              +    C+      +V VP VV HF G    V LP  NY       G         +G
Sbjct: 428 PSYSLFDTCFDLSGMTTVKVPTVVFHFGG--GEVSLPASNYLIPVNTEGRFCFAFAGTMG 483

Query: 430 CLMLMNGGDEAELAGGPGATLGNYQQQGFEVVYDLEKNRVGFARRQC 470
            L +                +GN QQQGF V YDL  +RVGF  R C
Sbjct: 488 SLSI----------------IGNIQQQGFRVAYDLVGSRVGFLSRAC 483

[Close]

BLAST of CSPI03G02720 vs. TAIR 10
Match: AT1G01300.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 152.1 bits (383), Expect = 1.1e-36
Identity = 133/409 (32.52%), Postives = 183/409 (44.74%), Query Frame = 0


Query: 70  LSPG-GDYTLSFNLGSESHKISLYMDTGSDLVWFPCSPFECILCEGKPKIQSPLPKIANN 129
           LS G G+Y     +G+ +  + + +DTGSD+VW  C+P  C  C  +       P     
Sbjct: 135 LSQGSGEYFTRLGVGTPARYVYMVLDTGSDIVWLQCAP--CRRCYSQSD-----PIFDPR 194

Query: 130 KSVSCSAAACSAAHGGSLSASHLCAISRCPLESIEISECSSFSCPPFYYAYGDGSL-VAR 189
           KS + +   CS+ H   L ++  C   R          C       +  +YGDGS  V  
Sbjct: 195 KSKTYATIPCSSPHCRRLDSAG-CNTRR--------KTCL------YQVSYGDGSFTVGD 254

Query: 190 LYRDSLSLPTPAPSPPINVRNFTFGCAHTTLGEPVGVA---GFGRGVLSMPSQLATFSPQ 249
              ++L+           V+    GC H   G  VG A   G G+G LS P Q      +
Sbjct: 255 FSTETLTFRRN------RVKGVALGCGHDNEGLFVGAAGLLGLGKGKLSFPGQT---GHR 314

Query: 250 LGNRFSYCLVSHSFAADRVRRPSPLILGRYYTGETEFIYTSLLENPKHPYFYSVGLAGIS 309
              +FSYCLV  S ++    +PS ++ G          +T LL NPK   FY VGL GIS
Sbjct: 315 FNQKFSYCLVDRSASS----KPSSVVFGNAAVSRIA-RFTPLLSNPKLDTFYYVGLLGIS 374

Query: 310 VGNVRIP-APEFLTKVDEGGSGGVVVDSGTTFTMLPAGLYESVVAEFENRTGKVANRARR 369
           VG  R+P     L K+D+ G+GGV++DSGT+ T L    Y ++   F  R G  A   +R
Sbjct: 375 VGGTRVPGVTASLFKLDQIGNGGVIIDSGTSVTRLIRPAYIAMRDAF--RVG--AKTLKR 434

Query: 370 IEENTGLSPCYYYE--NSVGVPRVVLHFVGEKSNVVLPRKNYFYEFLDGGDGVVGRKRKV 429
             + +    C+     N V VP VVLHF G  ++V LP  NY       G         +
Sbjct: 435 APDFSLFDTCFDLSNMNEVKVPTVVLHFRG--ADVSLPATNYLIPVDTNGKFCFAFAGTM 485

Query: 430 GCLMLMNGGDEAELAGGPGATLGNYQQQGFEVVYDLEKNRVGFARRQCS 471
           G L +                +GN QQQGF VVYDL  +RVGFA   C+
Sbjct: 495 GGLSI----------------IGNIQQQGFRVVYDLASSRVGFAPGGCA 485

The following BLAST results are available for this feature:

BLAST of CSPI03G02720 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q940R4	8.0e-165	62.34	Probable aspartyl protease At4g16563 OS=Arabidopsis thaliana OX=3702 GN=At4g1656...	[more]
Q9LNJ3	1.6e-35	32.52	Aspartyl protease family protein 2 OS=Arabidopsis thaliana OX=3702 GN=APF2 PE=2 ...	[more]
Q9LS40	2.5e-33	29.45	Protein ASPARTIC PROTEASE IN GUARD CELL 1 OS=Arabidopsis thaliana OX=3702 GN=ASP...	[more]
Q766C3	2.5e-33	29.80	Aspartic proteinase nepenthesin-1 OS=Nepenthes gracilis OX=150966 GN=nep1 PE=1 S...	[more]
Q766C2	2.5e-33	29.38	Aspartic proteinase nepenthesin-2 OS=Nepenthes gracilis OX=150966 GN=nep2 PE=1 S...	[more]

BLAST of CSPI03G02720 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0L5I7	5.9e-280	99.79	Pepsin A OS=Cucumis sativus OX=3659 GN=Csa_3G020060 PE=3 SV=1	[more]
A0A1S3BK28	3.7e-274	98.13	aspartic proteinase nepenthesin-1 OS=Cucumis melo OX=3656 GN=LOC103490888 PE=3 S...	[more]
A0A5D3CP11	2.7e-272	97.52	Aspartic proteinase nepenthesin-1 OS=Cucumis melo var. makuwa OX=1194695 GN=E567...	[more]
A0A6J1L3Z9	7.1e-249	90.00	probable aspartyl protease At4g16563 OS=Cucurbita maxima OX=3661 GN=LOC111500303...	[more]
A0A6J1EC44	2.7e-248	89.83	probable aspartyl protease At4g16563 OS=Cucurbita moschata OX=3662 GN=LOC1114312...	[more]

BLAST of CSPI03G02720 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_004147205.1	1.2e-279	99.79	probable aspartyl protease At4g16563 [Cucumis sativus]	[more]
XP_008448851.1	7.7e-274	98.13	PREDICTED: aspartic proteinase nepenthesin-1 [Cucumis melo]	[more]
TYK12019.1	5.5e-272	97.52	aspartic proteinase nepenthesin-1 [Cucumis melo var. makuwa]	[more]
XP_038905814.1	4.2e-256	91.88	probable aspartyl protease At4g16563 [Benincasa hispida]	[more]
KAE8650060.1	7.5e-253	99.77	hypothetical protein Csa_011084 [Cucumis sativus]	[more]

BLAST of CSPI03G02720 vs. TAIR 10


Match Name	E-value	Identity	Description
AT4G16563.1	5.7e-166	62.34	Eukaryotic aspartyl protease family protein	[more]
AT5G45120.1	1.3e-53	34.44	Eukaryotic aspartyl protease family protein	[more]
AT3G52500.1	1.4e-44	29.90	Eukaryotic aspartyl protease family protein	[more]
AT3G61820.1	8.5e-37	32.19	Eukaryotic aspartyl protease family protein	[more]
AT1G01300.1	1.1e-36	32.52	Eukaryotic aspartyl protease family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Cucumber (PI 183967) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR032799	Xylanase inhibitor, C-terminal	PFAM	PF14541	TAXi_C	coord: 295..465 e-value: 1.9E-28 score: 99.3
IPR021109	Aspartic peptidase domain superfamily	GENE3D	2.40.70.10	Acid Proteases	coord: 54..261 e-value: 7.3E-34 score: 119.4
IPR021109	Aspartic peptidase domain superfamily	GENE3D	2.40.70.10	Acid Proteases	coord: 272..475 e-value: 4.3E-49 score: 168.7
IPR021109	Aspartic peptidase domain superfamily	SUPERFAMILY	50630	Acid proteases	coord: 71..474
IPR032861	Xylanase inhibitor, N-terminal	PFAM	PF14543	TAXi_N	coord: 76..259 e-value: 1.8E-28 score: 100.0
None	No IPR available	PANTHER	PTHR47967	OS07G0603500 PROTEIN-RELATED	coord: 1..474
None	No IPR available	PANTHER	PTHR47967:SF26	BNAA01G17170D PROTEIN	coord: 1..474
IPR001969	Aspartic peptidase, active site	PROSITE	PS00141	ASP_PROTEASE	coord: 327..338
IPR033121	Peptidase family A1 domain	PROSITE	PS51767	PEPTIDASE_A1	coord: 76..465 score: 29.87694
IPR034161	Pepsin-like domain, plant	CDD	cd05476	pepsin_A_like_plant	coord: 76..469 e-value: 1.83697E-71 score: 225.605

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CSPI03G02720.1	CSPI03G02720.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006508	proteolysis
cellular_component	GO:0005576	extracellular region
molecular_function	GO:0004190	aspartic-type endopeptidase activity