CSPI02G25550 (gene) Cucumber (PI 183967) v1

Overview
NameCSPI02G25550
Typegene
OrganismCucumis sativus var. hardwickii cv. PI 183967 (Cucumber (PI 183967) v1)
DescriptionProtein of unknown function (DUF 3339)
LocationChr2: 21709890 .. 21710643 (-)
RNA-Seq ExpressionCSPI02G25550
SyntenyCSPI02G25550
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
ACCCCACTTATGAAAAAACAAAAAAAGAAAACATGTGAATCTGAATAACTTCATTCACTAAGCTTCAACTTTCCAAGTCATCCCTCCACCATAATTTTGTCCTTTTCTTCACCGCTATTAACACCTTAGCTTCCATTGCTTTTGATATGTTCCTGATCGTCTCTCTGTTGGTTTGTTGTTGAGAGGGAGAGGTACTATCGAGAAAGATCAATGGCGGATTGGGGTCCGGTTTTTGTAGCGGTGATGTTGTTCGTTCTGCTAACTCCGGGCTTGTTGGTTCAGATGCCTGGGAAGAGTCGGTTCGTTGAATTTGGGAATTTTCAGACGAGTGGAGTCTCCATTTTGGTTCATTCAATTCTCTATTTTGCATTGATCTGTATCTTCTTGTTGGCTGTTCGTGTTCATGTTTATATGGGCTCCTAATTTCACATCTTTCTCTCTATTTTTTTTCTTTCTTTCCCTTAAATCTGATTGTCTTTTGTCTTTAAACTTGTTTTGTATCCATTTTTTTTCTCAATCTCCTTTGATTTGTTAATAAGTATGTGTGGGTTTGTCATCTTTGAAGATGAGGAATTGGTATATAGCATCATTCTCTTAACTTTCTCTTCTATGTTTTTGAGGAATTTGATTCTTCAAATCTATCAATCCCATCCCATCACATCACATGTGCAAACATATATTTCGTCATGACAAAAAAAACTTTTTATCAATATTTTCAAAATATTTACGAATACGATAAAATTGATACACTATT

mRNA sequence

ACCCCACTTATGAAAAAACAAAAAAAGAAAACATGTGAATCTGAATAACTTCATTCACTAAGCTTCAACTTTCCAAGTCATCCCTCCACCATAATTTTGTCCTTTTCTTCACCGCTATTAACACCTTAGCTTCCATTGCTTTTGATATGTTCCTGATCGTCTCTCTGTTGGTTTGTTGTTGAGAGGGAGAGGTACTATCGAGAAAGATCAATGGCGGATTGGGGTCCGGTTTTTGTAGCGGTGATGTTGTTCGTTCTGCTAACTCCGGGCTTGTTGGTTCAGATGCCTGGGAAGAGTCGGTTCGTTGAATTTGGGAATTTTCAGACGAGTGGAGTCTCCATTTTGGTTCATTCAATTCTCTATTTTGCATTGATCTGTATCTTCTTGTTGGCTGTTCGTGTTCATGTTTATATGGGCTCCTAATTTCACATCTTTCTCTCTATTTTTTTTCTTTCTTTCCCTTAAATCTGATTGTCTTTTGTCTTTAAACTTGTTTTGTATCCATTTTTTTTCTCAATCTCCTTTGATTTGTTAATAAGTATGTGTGGGTTTGTCATCTTTGAAGATGAGGAATTGGTATATAGCATCATTCTCTTAACTTTCTCTTCTATGTTTTTGAGGAATTTGATTCTTCAAATCTATCAATCCCATCCCATCACATCACATGTGCAAACATATATTTCGTCATGACAAAAAAAACTTTTTATCAATATTTTCAAAATATTTACGAATACGATAAAATTGATACACTATT

Coding sequence (CDS)

ATGGCGGATTGGGGTCCGGTTTTTGTAGCGGTGATGTTGTTCGTTCTGCTAACTCCGGGCTTGTTGGTTCAGATGCCTGGGAAGAGTCGGTTCGTTGAATTTGGGAATTTTCAGACGAGTGGAGTCTCCATTTTGGTTCATTCAATTCTCTATTTTGCATTGATCTGTATCTTCTTGTTGGCTGTTCGTGTTCATGTTTATATGGGCTCCTAA

Protein sequence

MADWGPVFVAVMLFVLLTPGLLVQMPGKSRFVEFGNFQTSGVSILVHSILYFALICIFLLAVRVHVYMGS*
Homology
BLAST of CSPI02G25550 vs. ExPASy TrEMBL
Match: A0A5D3BUE1 (Uncharacterized protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold1154G00130 PE=4 SV=1)

HSP 1 Score: 138.7 bits (348), Expect = 9.7e-30
Identity = 70/70 (100.00%), Postives = 70/70 (100.00%), Query Frame = 0

Query: 1  MADWGPVFVAVMLFVLLTPGLLVQMPGKSRFVEFGNFQTSGVSILVHSILYFALICIFLL 60
          MADWGPVFVAVMLFVLLTPGLLVQMPGKSRFVEFGNFQTSGVSILVHSILYFALICIFLL
Sbjct: 1  MADWGPVFVAVMLFVLLTPGLLVQMPGKSRFVEFGNFQTSGVSILVHSILYFALICIFLL 60

Query: 61 AVRVHVYMGS 71
          AVRVHVYMGS
Sbjct: 61 AVRVHVYMGS 70

BLAST of CSPI02G25550 vs. ExPASy TrEMBL
Match: A0A6J1BYE9 (uncharacterized protein LOC111006599 OS=Momordica charantia OX=3673 GN=LOC111006599 PE=4 SV=1)

HSP 1 Score: 134.4 bits (337), Expect = 1.8e-28
Identity = 68/70 (97.14%), Postives = 69/70 (98.57%), Query Frame = 0

Query: 1  MADWGPVFVAVMLFVLLTPGLLVQMPGKSRFVEFGNFQTSGVSILVHSILYFALICIFLL 60
          MADWGPVFVAV+LFVLLTPGLLVQMPGKSRFVEFGNFQTSGVSILVHSILYFALICIFLL
Sbjct: 1  MADWGPVFVAVVLFVLLTPGLLVQMPGKSRFVEFGNFQTSGVSILVHSILYFALICIFLL 60

Query: 61 AVRVHVYMGS 71
          AV VHVYMGS
Sbjct: 61 AVGVHVYMGS 70

BLAST of CSPI02G25550 vs. ExPASy TrEMBL
Match: A0A6J1K207 (uncharacterized protein LOC111489412 OS=Cucurbita maxima OX=3661 GN=LOC111489412 PE=4 SV=1)

HSP 1 Score: 131.0 bits (328), Expect = 2.0e-27
Identity = 65/70 (92.86%), Postives = 68/70 (97.14%), Query Frame = 0

Query: 1  MADWGPVFVAVMLFVLLTPGLLVQMPGKSRFVEFGNFQTSGVSILVHSILYFALICIFLL 60
          MADWGPVFVAV+LFVLLTPGLLVQMPGK+RF+EFGNFQTSGVSILVHS LYFALICIFLL
Sbjct: 1  MADWGPVFVAVVLFVLLTPGLLVQMPGKNRFIEFGNFQTSGVSILVHSALYFALICIFLL 60

Query: 61 AVRVHVYMGS 71
          AV VHVYMGS
Sbjct: 61 AVGVHVYMGS 70

BLAST of CSPI02G25550 vs. ExPASy TrEMBL
Match: A0A6J1FLS0 (uncharacterized protein LOC111445120 OS=Cucurbita moschata OX=3662 GN=LOC111445120 PE=4 SV=1)

HSP 1 Score: 131.0 bits (328), Expect = 2.0e-27
Identity = 65/70 (92.86%), Postives = 68/70 (97.14%), Query Frame = 0

Query: 1  MADWGPVFVAVMLFVLLTPGLLVQMPGKSRFVEFGNFQTSGVSILVHSILYFALICIFLL 60
          MADWGPVFVAV+LFVLLTPGLLVQMPGK+RF+EFGNFQTSGVSILVHS LYFALICIFLL
Sbjct: 1  MADWGPVFVAVVLFVLLTPGLLVQMPGKNRFIEFGNFQTSGVSILVHSALYFALICIFLL 60

Query: 61 AVRVHVYMGS 71
          AV VHVYMGS
Sbjct: 61 AVGVHVYMGS 70

BLAST of CSPI02G25550 vs. ExPASy TrEMBL
Match: A0A540KW79 (Uncharacterized protein OS=Malus baccata OX=106549 GN=C1H46_035956 PE=4 SV=1)

HSP 1 Score: 129.0 bits (323), Expect = 7.7e-27
Identity = 61/70 (87.14%), Postives = 69/70 (98.57%), Query Frame = 0

Query: 1  MADWGPVFVAVMLFVLLTPGLLVQMPGKSRFVEFGNFQTSGVSILVHSILYFALICIFLL 60
          M+DWGPVF+AV+LFVLLTPGLL+QMPGKSRFVEFGNFQTSGVSILVHSI+YFALICIFLL
Sbjct: 1  MSDWGPVFIAVLLFVLLTPGLLIQMPGKSRFVEFGNFQTSGVSILVHSIIYFALICIFLL 60

Query: 61 AVRVHVYMGS 71
          A+ VH+Y+GS
Sbjct: 61 AIGVHMYVGS 70

BLAST of CSPI02G25550 vs. NCBI nr
Match: KAA0056547.1 (uncharacterized protein E6C27_scaffold288G00490 [Cucumis melo var. makuwa] >TYK02715.1 uncharacterized protein E5676_scaffold1154G00130 [Cucumis melo var. makuwa])

HSP 1 Score: 138.7 bits (348), Expect = 2.0e-29
Identity = 70/70 (100.00%), Postives = 70/70 (100.00%), Query Frame = 0

Query: 1  MADWGPVFVAVMLFVLLTPGLLVQMPGKSRFVEFGNFQTSGVSILVHSILYFALICIFLL 60
          MADWGPVFVAVMLFVLLTPGLLVQMPGKSRFVEFGNFQTSGVSILVHSILYFALICIFLL
Sbjct: 1  MADWGPVFVAVMLFVLLTPGLLVQMPGKSRFVEFGNFQTSGVSILVHSILYFALICIFLL 60

Query: 61 AVRVHVYMGS 71
          AVRVHVYMGS
Sbjct: 61 AVRVHVYMGS 70

BLAST of CSPI02G25550 vs. NCBI nr
Match: XP_022134304.1 (uncharacterized protein LOC111006599 [Momordica charantia])

HSP 1 Score: 134.4 bits (337), Expect = 3.8e-28
Identity = 68/70 (97.14%), Postives = 69/70 (98.57%), Query Frame = 0

Query: 1  MADWGPVFVAVMLFVLLTPGLLVQMPGKSRFVEFGNFQTSGVSILVHSILYFALICIFLL 60
          MADWGPVFVAV+LFVLLTPGLLVQMPGKSRFVEFGNFQTSGVSILVHSILYFALICIFLL
Sbjct: 1  MADWGPVFVAVVLFVLLTPGLLVQMPGKSRFVEFGNFQTSGVSILVHSILYFALICIFLL 60

Query: 61 AVRVHVYMGS 71
          AV VHVYMGS
Sbjct: 61 AVGVHVYMGS 70

BLAST of CSPI02G25550 vs. NCBI nr
Match: XP_011649954.2 (uncharacterized protein LOC101211888 [Cucumis sativus])

HSP 1 Score: 134.0 bits (336), Expect = 4.9e-28
Identity = 68/69 (98.55%), Postives = 68/69 (98.55%), Query Frame = 0

Query: 1  MADWGPVFVAVMLFVLLTPGLLVQMPGKSRFVEFGNFQTSGVSILVHSILYFALICIFLL 60
          MADWGPVFVAVMLFVLLTPGLLVQMPGKSRFVEFGNFQTSGVSILVHSILYFALICIFLL
Sbjct: 1  MADWGPVFVAVMLFVLLTPGLLVQMPGKSRFVEFGNFQTSGVSILVHSILYFALICIFLL 60

Query: 61 AVRVHVYMG 70
          AVRVHVY G
Sbjct: 61 AVRVHVYNG 69

BLAST of CSPI02G25550 vs. NCBI nr
Match: XP_023551540.1 (uncharacterized protein LOC111809330 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 131.0 bits (328), Expect = 4.2e-27
Identity = 65/70 (92.86%), Postives = 68/70 (97.14%), Query Frame = 0

Query: 1  MADWGPVFVAVMLFVLLTPGLLVQMPGKSRFVEFGNFQTSGVSILVHSILYFALICIFLL 60
          MADWGPVFVAV+LFVLLTPGLLVQMPGK+RF+EFGNFQTSGVSILVHS LYFALICIFLL
Sbjct: 1  MADWGPVFVAVVLFVLLTPGLLVQMPGKNRFIEFGNFQTSGVSILVHSALYFALICIFLL 60

Query: 61 AVRVHVYMGS 71
          AV VHVYMGS
Sbjct: 61 AVGVHVYMGS 70

BLAST of CSPI02G25550 vs. NCBI nr
Match: XP_022993368.1 (uncharacterized protein LOC111489412 [Cucurbita maxima])

HSP 1 Score: 131.0 bits (328), Expect = 4.2e-27
Identity = 65/70 (92.86%), Postives = 68/70 (97.14%), Query Frame = 0

Query: 1  MADWGPVFVAVMLFVLLTPGLLVQMPGKSRFVEFGNFQTSGVSILVHSILYFALICIFLL 60
          MADWGPVFVAV+LFVLLTPGLLVQMPGK+RF+EFGNFQTSGVSILVHS LYFALICIFLL
Sbjct: 1  MADWGPVFVAVVLFVLLTPGLLVQMPGKNRFIEFGNFQTSGVSILVHSALYFALICIFLL 60

Query: 61 AVRVHVYMGS 71
          AV VHVYMGS
Sbjct: 61 AVGVHVYMGS 70

BLAST of CSPI02G25550 vs. TAIR 10
Match: AT5G08391.1 (Protein of unknown function (DUF 3339) )

HSP 1 Score: 110.5 bits (275), Expect = 5.5e-25
Identity = 46/69 (66.67%), Postives = 64/69 (92.75%), Query Frame = 0

Query: 1  MADWGPVFVAVMLFVLLTPGLLVQMPGKSRFVEFGNFQTSGVSILVHSILYFALICIFLL 60
          M+DWGPV V V+LFV+LTPGLL Q+PG+ ++VEFGNFQTS VS++VHS+LYF+L+C+FLL
Sbjct: 1  MSDWGPVLVTVILFVMLTPGLLFQLPGRQKYVEFGNFQTSAVSVIVHSLLYFSLVCVFLL 60

Query: 61 AVRVHVYMG 70
          A+++H+Y+G
Sbjct: 61 ALKIHIYIG 69

BLAST of CSPI02G25550 vs. TAIR 10
Match: AT3G27030.1 (unknown protein; FUNCTIONS IN: molecular_function unknown; INVOLVED IN: biological_process unknown; LOCATED IN: endomembrane system; CONTAINS InterPro DOMAIN/s: Protein of unknown function DUF3339 (InterPro:IPR021775); BEST Arabidopsis thaliana protein match is: Protein of unknown function (DUF 3339) (TAIR:AT5G40970.1); Has 538 Blast hits to 271 proteins in 16 species: Archae - 0; Bacteria - 0; Metazoa - 0; Fungi - 0; Plants - 534; Viruses - 4; Other Eukaryotes - 0 (source: NCBI BLink). )

HSP 1 Score: 102.8 bits (255), Expect = 1.1e-22
Identity = 45/66 (68.18%), Postives = 59/66 (89.39%), Query Frame = 0

Query: 1  MADWGPVFVAVMLFVLLTPGLLVQMPGKSRFVEFGNFQTSGVSILVHSILYFALICIFLL 60
          M DWGPVFVAV LFVLLTPGLL+Q+PG+ R VEFG FQTSG+S++VH+++YF L+CI LL
Sbjct: 1  MPDWGPVFVAVTLFVLLTPGLLIQVPGRGRVVEFGTFQTSGLSVIVHTLIYFTLVCILLL 60

Query: 61 AVRVHV 67
          A+++H+
Sbjct: 61 ALQIHI 66

BLAST of CSPI02G25550 vs. TAIR 10
Match: AT3G48660.1 (Protein of unknown function (DUF 3339) )

HSP 1 Score: 102.4 bits (254), Expect = 1.5e-22
Identity = 48/70 (68.57%), Postives = 59/70 (84.29%), Query Frame = 0

Query: 1  MADWGPVFVAVMLFVLLTPGLLVQMPGKSRFVEFGNFQTSGVSILVHSILYFALICIFLL 60
          MADWGPV VAV+LFVLLTPGLL Q+P + R VEFGN QTSG SILVH+I++F LI IF +
Sbjct: 1  MADWGPVVVAVILFVLLTPGLLFQIPARGRVVEFGNMQTSGASILVHTIIFFGLITIFTI 60

Query: 61 AVRVHVYMGS 71
          A+R+H+Y G+
Sbjct: 61 AIRLHIYTGT 70

BLAST of CSPI02G25550 vs. TAIR 10
Match: AT5G63500.1 (Protein of unknown function (DUF 3339) )

HSP 1 Score: 96.7 bits (239), Expect = 8.2e-21
Identity = 45/69 (65.22%), Postives = 56/69 (81.16%), Query Frame = 0

Query: 1  MADWGPVFVAVMLFVLLTPGLLVQMPGKSRFVEFGNFQTSGVSILVHSILYFALICIFLL 60
          MADWGPV +AV+LFV+L+PGLL Q+P   R VEFGN QTSG SILVH+I++F LI IF +
Sbjct: 1  MADWGPVLIAVILFVVLSPGLLFQIPAGGRVVEFGNMQTSGASILVHAIIFFGLITIFTI 60

Query: 61 AVRVHVYMG 70
          A+ VH+Y G
Sbjct: 61 AINVHIYSG 69

BLAST of CSPI02G25550 vs. TAIR 10
Match: AT5G40980.1 (Protein of unknown function (DUF 3339) )

HSP 1 Score: 90.1 bits (222), Expect = 7.6e-19
Identity = 38/66 (57.58%), Postives = 56/66 (84.85%), Query Frame = 0

Query: 2  ADWGPVFVAVMLFVLLTPGLLVQMPGKSRFVEFGNFQTSGVSILVHSILYFALICIFLLA 61
          ADWGPV VAV LF+LL+PGLL Q+P ++R +EFGN  TSG+SILVH+I+YF ++ I ++A
Sbjct: 3  ADWGPVIVAVALFILLSPGLLFQLPARTRVMEFGNMSTSGISILVHAIIYFCILTILIIA 62

Query: 62 VRVHVY 68
          +++H++
Sbjct: 63 IQIHIH 68

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Match NameE-valueIdentityDescription
A0A5D3BUE19.7e-30100.00Uncharacterized protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold... [more]
A0A6J1BYE91.8e-2897.14uncharacterized protein LOC111006599 OS=Momordica charantia OX=3673 GN=LOC111006... [more]
A0A6J1K2072.0e-2792.86uncharacterized protein LOC111489412 OS=Cucurbita maxima OX=3661 GN=LOC111489412... [more]
A0A6J1FLS02.0e-2792.86uncharacterized protein LOC111445120 OS=Cucurbita moschata OX=3662 GN=LOC1114451... [more]
A0A540KW797.7e-2787.14Uncharacterized protein OS=Malus baccata OX=106549 GN=C1H46_035956 PE=4 SV=1[more]
Match NameE-valueIdentityDescription
KAA0056547.12.0e-29100.00uncharacterized protein E6C27_scaffold288G00490 [Cucumis melo var. makuwa] >TYK0... [more]
XP_022134304.13.8e-2897.14uncharacterized protein LOC111006599 [Momordica charantia][more]
XP_011649954.24.9e-2898.55uncharacterized protein LOC101211888 [Cucumis sativus][more]
XP_023551540.14.2e-2792.86uncharacterized protein LOC111809330 [Cucurbita pepo subsp. pepo][more]
XP_022993368.14.2e-2792.86uncharacterized protein LOC111489412 [Cucurbita maxima][more]
Match NameE-valueIdentityDescription
AT5G08391.15.5e-2566.67Protein of unknown function (DUF 3339) [more]
AT3G27030.11.1e-2268.18unknown protein; FUNCTIONS IN: molecular_function unknown; INVOLVED IN: biologic... [more]
AT3G48660.11.5e-2268.57Protein of unknown function (DUF 3339) [more]
AT5G63500.18.2e-2165.22Protein of unknown function (DUF 3339) [more]
AT5G40980.17.6e-1957.58Protein of unknown function (DUF 3339) [more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (PI 183967) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR021775Protein of unknown function DUF3339PFAMPF11820DUF3339coord: 1..67
e-value: 1.6E-35
score: 121.1
IPR021775Protein of unknown function DUF3339PANTHERPTHR33128OS05G0103400 PROTEINcoord: 1..69
NoneNo IPR availablePANTHERPTHR33128:SF71BNAC07G48990D PROTEINcoord: 1..69

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CSPI02G25550.1CSPI02G25550.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
cellular_component GO:0016021 integral component of membrane