CSPI01G18070 (gene) Cucumber (PI 183967) v1

Overview
NameCSPI01G18070
Typegene
OrganismCucumis sativus var. hardwickii cv. PI 183967 (Cucumber (PI 183967) v1)
DescriptionCinnamoyl-CoA reductase 1
LocationChr1: 13563192 .. 13565489 (-)
RNA-Seq ExpressionCSPI01G18070
SyntenyCSPI01G18070
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
ATTCAACTAATGACAAAATGTGTTTAGAGAACATTTAGAATCGCTTTTTAAAGGGAGAGTGCTATAACGTGGGTTTTAAAACTTTATGGACCAAAATGTATTTTATCCGTAAATAAAAATCTCAATGAAAAACCACTTTCTCCACCGTATAAACATGATTTAATAGATTACATATTCCTCTTAGGAGCACCTATAACAAACTTCCCCCCTTGAAACAATGTCATAGCAGTCGACAAAATTTGCATTTCTTTCCACTTCTCCAAATTTTCACCTTCATTTCATGCCAATTACTTCACCACACATCTCCTCATCTCTATAAAATCATCCCCATTTCTCACAAACACAGAAATACACGAAACAGAGACACCCAAAAATGGATCATTCAAAGCCAACTCTTTGCGTTCTTGACGCTTCCTCTTACGTCGGATTTTGGGTCACCAAAGGATTGCTAAACAAAGGATACCCAGTTCATGCAGCCGTCCAGAACACTGGCAAGTCATTTCTTTTATCCTCTGTTCTTTTCCGCTTCTTAATTCTCTGTTTGTATTTCAAACTTGTGGGCTTGATCTTTACAGGAAAGCCTGAGATTGAAAAGACTATTAGAGAGATGGGTAAAGTGGAGAATGGGCTGGCGGTTTTTTCTGTAGATATCATGGATTATCAGAGTATTCTTGTTGCTTTGAAAGGGTGTTCTGGTTTGTTCTGTTGTATGGACACCCACCATGTGTATGATGTAAGTCCAAACAGAAAAAGAAGGAATTAAAAGATTATCTAGGATTTGTGGGTTATTGTTGTTTTGTGTTTTTTTTTTTTTTTTTTTTTTTGCAGGAAAAAATGGTGGATTTGGAGGTTAGAGGAACAATTAATGTCGTCGAGGCTTGTGCTCAGACAGATAGTGTTGAAAAGATTGTCTTCACATCCTCCTTAACTGCTGCTGTGTGGAGAGAGAATATCCTCTCTGAGAAAGATGTGGATGAAAGATGTTGGAGTGATAAAGAATTTTGCAGGAAAATGAAGGTTGGGAATTTCAAGTCCATCTTCTCATCTTTTCCCCTTAATCATTTTGGATTTCTTCATGAAGCTCCTCAAATATCAGATGCCTTCTCAATCAATTTGACTACCATTCATATGCCGAAATGCTTTCTATCTGTTATCTTCTTTCGAACTATCAAGTACCAAGATCCAAGTCCTAGTTGCATTCGGGACAACCCAAAACTTAAGCTATTTTCATATATGTTATATATTAACGTTATTTATTATTTTGACCATAGAGGTTAGAGAAGTGCATTAAATGAAATCTTTGAGCCAATGAGATCTGAATTATTCTATCTAATACTTTGCTTTTGACATTTTTATGCTTATTTTTATCTCTACTTTATAACATCAAGCTTTTTCTTCACAGCTCAAATGACCCTTTTTATTTTATATCATCTCCCAGTTGTGGTATCCTTTAGCAAAGACACTTTCTGAGCAAGCAGCGTGGGCTCTTGCAATGGACCGAAGGCTAAAAATGGTATCAATTAACGCAGGACTTGTTCTTGGACCTGCTGTTGCAGAAGAGAACTCAGGCTCAACAATCTCATATCTTAAAGGTAGGTGAAAGTGCTTTACCAAAGATATGTACTATTATGGGATCCTTTTTGTTTTGACAAAGCAAATTTGAGGGGAAACAGGAGCTGCTCAGATGTATGAAAATGGGGTTCTAGCTGTGGTTGATGTGAAGTTTTTGGTGGATGTTCATATTCGAGCAATGGAAGATTCGTCGACGGGTGGTAGATATTTCTGCTTCGATCAAATAGTGAATAGTGAGGAAGAAACTGTTAAACTTGCGAATATATTGAGGCCATTGATATCTCTCCCACAAAGGTAAAAAGCACTATTATTAGCCCTTTGAACAACCAAAACACAGCCTTTTTTAAGTTCATTTGTCGCTTTGCCATTTGCATGTTATTATTGTTTACTTATTAATTGAAACTCAAAAGTTTATTGGTTGGTGAGTAGGTACGAAGTTCAAGGAAGGGAAGCTTTTGCTGAGAGACTCAGAACTAGGAAATTGCACAAACTCATCGAAGGCACTGCTTAATACTATACTAAATTTCACAACTTTAGGAAAAAAGATCCACTTTTTTTCCATTTTTTTTCTTCTTCTATTGGTATCTATCTTTGTTAGCACTTAATATAATGAAGAAAAAAAAAAACTTCTATTTAGCGTGTACGCTGACGAGAGACATCATATTCATATTAGTCATTATTTACGTCTATTATCAATGTTACCATTTTAAAAATTGACAATTCAAA

mRNA sequence

ATTCAACTAATGACAAAATGTGTTTAGAGAACATTTAGAATCGCTTTTTAAAGGGAGAGTGCTATAACGTGGGTTTTAAAACTTTATGGACCAAAATGTATTTTATCCGTAAATAAAAATCTCAATGAAAAACCACTTTCTCCACCGTATAAACATGATTTAATAGATTACATATTCCTCTTAGGAGCACCTATAACAAACTTCCCCCCTTGAAACAATGTCATAGCAGTCGACAAAATTTGCATTTCTTTCCACTTCTCCAAATTTTCACCTTCATTTCATGCCAATTACTTCACCACACATCTCCTCATCTCTATAAAATCATCCCCATTTCTCACAAACACAGAAATACACGAAACAGAGACACCCAAAAATGGATCATTCAAAGCCAACTCTTTGCGTTCTTGACGCTTCCTCTTACGTCGGATTTTGGGTCACCAAAGGATTGCTAAACAAAGGATACCCAGTTCATGCAGCCGTCCAGAACACTGGAAAGCCTGAGATTGAAAAGACTATTAGAGAGATGGGTAAAGTGGAGAATGGGCTGGCGGTTTTTTCTGTAGATATCATGGATTATCAGAGTATTCTTGTTGCTTTGAAAGGGTGTTCTGGTTTGTTCTGTTGTATGGACACCCACCATGTGTATGATGAAAAAATGGTGGATTTGGAGGTTAGAGGAACAATTAATGTCGTCGAGGCTTGTGCTCAGACAGATAGTGTTGAAAAGATTGTCTTCACATCCTCCTTAACTGCTGCTGTGTGGAGAGAGAATATCCTCTCTGAGAAAGATGTGGATGAAAGATGTTGGAGTGATAAAGAATTTTGCAGGAAAATGAAGTTGTGGTATCCTTTAGCAAAGACACTTTCTGAGCAAGCAGCGTGGGCTCTTGCAATGGACCGAAGGCTAAAAATGGTATCAATTAACGCAGGACTTGTTCTTGGACCTGCTGTTGCAGAAGAGAACTCAGGCTCAACAATCTCATATCTTAAAGGAGCTGCTCAGATGTATGAAAATGGGGTTCTAGCTGTGGTTGATGTGAAGTTTTTGGTGGATGTTCATATTCGAGCAATGGAAGATTCGTCGACGGGTGGTAGATATTTCTGCTTCGATCAAATAGTGAATAGTGAGGAAGAAACTGTTAAACTTGCGAATATATTGAGGCCATTGATATCTCTCCCACAAAGGTACGAAGTTCAAGGAAGGGAAGCTTTTGCTGAGAGACTCAGAACTAGGAAATTGCACAAACTCATCGAAGGCACTGCTTAATACTATACTAAATTTCACAACTTTAGGAAAAAAGATCCACTTTTTTTCCATTTTTTTTCTTCTTCTATTGGTATCTATCTTTGTTAGCACTTAATATAATGAAGAAAAAAAAAAACTTCTATTTAGCGTGTACGCTGACGAGAGACATCATATTCATATTAGTCATTATTTACGTCTATTATCAATGTTACCATTTTAAAAATTGACAATTCAAA

Coding sequence (CDS)

ATGGATCATTCAAAGCCAACTCTTTGCGTTCTTGACGCTTCCTCTTACGTCGGATTTTGGGTCACCAAAGGATTGCTAAACAAAGGATACCCAGTTCATGCAGCCGTCCAGAACACTGGAAAGCCTGAGATTGAAAAGACTATTAGAGAGATGGGTAAAGTGGAGAATGGGCTGGCGGTTTTTTCTGTAGATATCATGGATTATCAGAGTATTCTTGTTGCTTTGAAAGGGTGTTCTGGTTTGTTCTGTTGTATGGACACCCACCATGTGTATGATGAAAAAATGGTGGATTTGGAGGTTAGAGGAACAATTAATGTCGTCGAGGCTTGTGCTCAGACAGATAGTGTTGAAAAGATTGTCTTCACATCCTCCTTAACTGCTGCTGTGTGGAGAGAGAATATCCTCTCTGAGAAAGATGTGGATGAAAGATGTTGGAGTGATAAAGAATTTTGCAGGAAAATGAAGTTGTGGTATCCTTTAGCAAAGACACTTTCTGAGCAAGCAGCGTGGGCTCTTGCAATGGACCGAAGGCTAAAAATGGTATCAATTAACGCAGGACTTGTTCTTGGACCTGCTGTTGCAGAAGAGAACTCAGGCTCAACAATCTCATATCTTAAAGGAGCTGCTCAGATGTATGAAAATGGGGTTCTAGCTGTGGTTGATGTGAAGTTTTTGGTGGATGTTCATATTCGAGCAATGGAAGATTCGTCGACGGGTGGTAGATATTTCTGCTTCGATCAAATAGTGAATAGTGAGGAAGAAACTGTTAAACTTGCGAATATATTGAGGCCATTGATATCTCTCCCACAAAGGTACGAAGTTCAAGGAAGGGAAGCTTTTGCTGAGAGACTCAGAACTAGGAAATTGCACAAACTCATCGAAGGCACTGCTTAA

Protein sequence

MDHSKPTLCVLDASSYVGFWVTKGLLNKGYPVHAAVQNTGKPEIEKTIREMGKVENGLAVFSVDIMDYQSILVALKGCSGLFCCMDTHHVYDEKMVDLEVRGTINVVEACAQTDSVEKIVFTSSLTAAVWRENILSEKDVDERCWSDKEFCRKMKLWYPLAKTLSEQAAWALAMDRRLKMVSINAGLVLGPAVAEENSGSTISYLKGAAQMYENGVLAVVDVKFLVDVHIRAMEDSSTGGRYFCFDQIVNSEEETVKLANILRPLISLPQRYEVQGREAFAERLRTRKLHKLIEGTA*
Homology
BLAST of CSPI01G18070 vs. ExPASy Swiss-Prot
Match: A0A059TC02 (Cinnamoyl-CoA reductase 1 OS=Petunia hybrida OX=4102 GN=CCR1 PE=1 SV=1)

HSP 1 Score: 164.1 bits (414), Expect = 2.5e-39
Identity = 96/271 (35.42%), Postives = 143/271 (52.77%), Query Frame = 0

Query: 4   SKPTLCVLDASSYVGFWVTKGLLNKGYPVHAAVQNTGKPEIEKTIREMGKVENGLAVFSV 63
           S   +CV  A  ++  W+ K LL KGY V   V+N   P+    +RE+   +  L +   
Sbjct: 5   SGQVVCVTGAGGFIASWLVKILLEKGYTVRGTVRNPDDPK-NGHLRELEGAKERLTLCKA 64

Query: 64  DIMDYQSILVALKGCSGLFCCMDTHHVYDEKMVDLEVRGTINVVEACAQTDSVEKIVFTS 123
           D++DYQS+  A+ GC G+F          E+MV+  V GT NV+ A A+  +V ++VFTS
Sbjct: 65  DLLDYQSLREAINGCDGVFHTASPVTDDPEQMVEPAVIGTKNVINAAAEA-NVRRVVFTS 124

Query: 124 SLTAAVWRENILSEKDVDERCWSDKEFCRKMKLWYPLAKTLSEQAAWALAMDRRLKMVSI 183
           S+ A     N   E  VDE CWSD +FC+  K WY   K ++EQAAW  A ++ + +V I
Sbjct: 125 SIGAVYMDPNRDPETVVDETCWSDPDFCKNTKNWYCYGKMVAEQAAWEEAKEKGVDLVVI 184

Query: 184 NAGLVLGPAVAEENSGST---ISYLKGAAQMYENGVLAVVDVKFLVDVHIRAMEDSSTGG 243
           N  LV GP +    + S    + YL G+A+ Y N V A VDVK +   HI   E     G
Sbjct: 185 NPVLVQGPLLQTTVNASVLHILKYLTGSAKTYANSVQAYVDVKDVALAHILLYETPEASG 244

Query: 244 RYFCFDQIVNSEEETVKLANILRPLISLPQR 272
           RY C + +++   + V++ +   P   +P +
Sbjct: 245 RYLCAESVLH-RGDVVEILSKFFPEYPIPTK 272

BLAST of CSPI01G18070 vs. ExPASy Swiss-Prot
Match: A0A0B6VQ48 (Phenylacetaldehyde reductase OS=Rosa hybrid cultivar OX=128735 GN=PAR PE=1 SV=1)

HSP 1 Score: 157.9 bits (398), Expect = 1.8e-37
Identity = 88/276 (31.88%), Postives = 157/276 (56.88%), Query Frame = 0

Query: 4   SKPTLCVLDASSYVGFWVTKGLLNKGYPVHAAVQNTGKPEIEKTIREMGKVENGLAVFSV 63
           S   +CV  AS Y+  W+ K LL +GY V A+V+N   P   + +  +   +  L +F  
Sbjct: 2   SNKVVCVTGASGYIASWLVKLLLQRGYTVKASVRNPNDPTKTEHLLALDGAKERLQLFKA 61

Query: 64  DIMDYQSILVALKGCSGLFCCMDT--HHVYDEK--MVDLEVRGTINVVEACAQTDSVEKI 123
           D+++  S   A++GC G+F       H V D K  ++D  V+GT+NV+ +C+++ S++++
Sbjct: 62  DLLEEGSFDSAVEGCEGVFHTASPFYHDVTDPKAELLDPAVKGTLNVLNSCSKSPSIKRV 121

Query: 124 VFTSSLTAAVWR-ENILSEKDVDERCWSDKEFCRKMKLWYPLAKTLSEQAAWALAMDRRL 183
           V TSS+ A  +  +    +  VDE  ++D + C++ KLWY L+KTL+E AAW    ++ +
Sbjct: 122 VLTSSIAAVAYNGKPRTPDVVVDETWFTDPDVCKESKLWYVLSKTLAEDAAWKFVKEKGI 181

Query: 184 KMVSINAGLVLGPAV---AEENSGSTISYLKGAAQMYENGVLAVVDVKFLVDVHIRAMED 243
            MV+IN  +V+GP +      ++ + ++ +KG A+ Y N     ++VK + + H++A E 
Sbjct: 182 DMVTINPAMVIGPLLQPTLNTSAAAILNIIKG-ARTYPNASFGWINVKDVANAHVQAFEI 241

Query: 244 SSTGGRYFCFDQIVNSEEETVKLANILRPLISLPQR 272
            S  GRY C  + V    E +++ + L P + LP++
Sbjct: 242 PSASGRY-CLVERVAHFTEVLQIIHELYPDLQLPEK 275

BLAST of CSPI01G18070 vs. ExPASy Swiss-Prot
Match: Q9S9N9 (Cinnamoyl-CoA reductase 1 OS=Arabidopsis thaliana OX=3702 GN=CCR1 PE=1 SV=1)

HSP 1 Score: 150.6 bits (379), Expect = 2.8e-35
Identity = 91/268 (33.96%), Postives = 136/268 (50.75%), Query Frame = 0

Query: 7   TLCVLDASSYVGFWVTKGLLNKGYPVHAAVQNTGKPEIEKTIREMGKVENGLAVFSVDIM 66
           T+CV  A  Y+  W+ K LL +GY V   V+N   P+    +RE+   +  L +   D+ 
Sbjct: 12  TVCVTGAGGYIASWIVKILLERGYTVKGTVRNPDDPK-NTHLRELEGGKERLILCKADLQ 71

Query: 67  DYQSILVALKGCSGLFCCMDTHHVYDEKMVDLEVRGTINVVEACAQTDSVEKIVFTSSLT 126
           DY+++  A+ GC G+F          E+MV+  V G   V+ A A+   V+++V TSS+ 
Sbjct: 72  DYEALKAAIDGCDGVFHTASPVTDDPEQMVEPAVNGAKFVINAAAEA-KVKRVVITSSIG 131

Query: 127 AAVWRENILSEKDVDERCWSDKEFCRKMKLWYPLAKTLSEQAAWALAMDRRLKMVSINAG 186
           A     N   E  VDE CWSD +FC+  K WY   K ++EQAAW  A ++ + +V +N  
Sbjct: 132 AVYMDPNRDPEAVVDESCWSDLDFCKNTKNWYCYGKMVAEQAAWETAKEKGVDLVVLNPV 191

Query: 187 LVLGPAVAEENSGS---TISYLKGAAQMYENGVLAVVDVKFLVDVHIRAMEDSSTGGRYF 246
           LVLGP +    + S    + YL G+A+ Y N   A VDV+ +   H+   E  S  GRY 
Sbjct: 192 LVLGPPLQPTINASLYHVLKYLTGSAKTYANLTQAYVDVRDVALAHVLVYEAPSASGRYL 251

Query: 247 CFDQIVNSEEETVKLANILRPLISLPQR 272
             +       E V++   L P   LP +
Sbjct: 252 LAES-ARHRGEVVEILAKLFPEYPLPTK 276

BLAST of CSPI01G18070 vs. ExPASy Swiss-Prot
Match: Q9SAH9 (Cinnamoyl-CoA reductase 2 OS=Arabidopsis thaliana OX=3702 GN=CCR2 PE=1 SV=1)

HSP 1 Score: 149.8 bits (377), Expect = 4.8e-35
Identity = 88/267 (32.96%), Postives = 136/267 (50.94%), Query Frame = 0

Query: 8   LCVLDASSYVGFWVTKGLLNKGYPVHAAVQNTGKPEIEKTIREMGKVENGLAVFSVDIMD 67
           +CV  A  Y+  W+ K LL +GY V   V+N   P+    +RE+   +  L + S D++D
Sbjct: 8   VCVTGAGGYIASWIVKLLLERGYTVRGTVRNPTDPK-NNHLRELQGAKERLTLHSADLLD 67

Query: 68  YQSILVALKGCSGLFCCMDTHHVYDEKMVDLEVRGTINVVEACAQTDSVEKIVFTSSLTA 127
           Y+++   + GC G+F          E M++  V G   V++A A+   V+++VFTSS+ A
Sbjct: 68  YEALCATIDGCDGVFHTASPMTDDPETMLEPAVNGAKFVIDAAAKA-KVKRVVFTSSIGA 127

Query: 128 AVWRENILSEKDVDERCWSDKEFCRKMKLWYPLAKTLSEQAAWALAMDRRLKMVSINAGL 187
                N  ++  VDE CWSD +FC+  K WY   K L+EQ+AW  A  + + +V +N  L
Sbjct: 128 VYMNPNRDTQAIVDENCWSDLDFCKNTKNWYCYGKMLAEQSAWETAKAKGVDLVVLNPVL 187

Query: 188 VLGPAVAEENSGSTI---SYLKGAAQMYENGVLAVVDVKFLVDVHIRAMEDSSTGGRYFC 247
           VLGP +    + S +    YL G+A+ Y N     VDV+ +   H+   E  S  GRY  
Sbjct: 188 VLGPPLQSAINASLVHILKYLTGSAKTYANLTQVYVDVRDVALGHVLVYEAPSASGRYIL 247

Query: 248 FDQIVNSEEETVKLANILRPLISLPQR 272
            +  ++  E    LA    P   LP +
Sbjct: 248 AETALHRGEVVEILAKFF-PEYPLPTK 271

BLAST of CSPI01G18070 vs. ExPASy Swiss-Prot
Match: Q500U8 (Tetraketide alpha-pyrone reductase 1 OS=Arabidopsis thaliana OX=3702 GN=TKPR1 PE=1 SV=1)

HSP 1 Score: 142.5 bits (358), Expect = 7.7e-33
Identity = 81/288 (28.12%), Postives = 151/288 (52.43%), Query Frame = 0

Query: 1   MDHSKPTLCVLDASSYVGFWVTKGLLNKGYPVHAAVQNTGKPEIEKTIREMGKVENG--- 60
           MD +K  +CV  AS ++  W+ K LL +GY V   V++ G    EK +  + K+E     
Sbjct: 1   MDQAKGKVCVTGASGFLASWLVKRLLLEGYEVIGTVRDPGN---EKKLAHLWKLEGAKER 60

Query: 61  LAVFSVDIMDYQSILVALKGCSGLF----CCMDTHHVYDEKMVDLEVRGTINVVEACAQT 120
           L +   D+M+  S   A+ GC G+F      +      +E+++   + GT+NV+ +C + 
Sbjct: 61  LRLVKADLMEEGSFDNAIMGCQGVFHTASPVLKPTSNPEEEILRPAIEGTLNVLRSCRKN 120

Query: 121 DSVEKIVFTSSLTAAVWRENILSEKDVDERCWSDKEFCRKMKLWYPLAKTLSEQAAWALA 180
            S++++V TSS +    R++   +  +DE  W+  E C++ ++WY L+KTL+EQAAW  +
Sbjct: 121 PSLKRVVLTSSSSTVRIRDDFDPKIPLDESIWTSVELCKRFQVWYALSKTLAEQAAWKFS 180

Query: 181 MDRRLKMVSINAGLVLGPAVAEE---NSGSTISYLKGAAQMYE-NGVLAVVDVKFLVDVH 240
            +  + +V++    ++GP++  +    +   +  LKG  + ++ +G +  V +  +   H
Sbjct: 181 EENGIDLVTVLPSFLVGPSLPPDLCSTASDVLGLLKGETEKFQWHGQMGYVHIDDVARTH 240

Query: 241 IRAMEDSSTGGRYFCFDQIVNSEEETVKLANILRPLISLPQRYEVQGR 278
           I   E  +  GRY C   ++ S EE V   +   P + +P+R+E   R
Sbjct: 241 IVVFEHEAAQGRYICSSNVI-SLEELVSFLSARYPSLPIPKRFEKLNR 284

BLAST of CSPI01G18070 vs. ExPASy TrEMBL
Match: A0A0A0LW90 (Cinnamoyl-CoA reductase 1 OS=Cucumis sativus OX=3659 GN=CCR1 PE=2 SV=1)

HSP 1 Score: 595.9 bits (1535), Expect = 9.3e-167
Identity = 295/297 (99.33%), Postives = 297/297 (100.00%), Query Frame = 0

Query: 1   MDHSKPTLCVLDASSYVGFWVTKGLLNKGYPVHAAVQNTGKPEIEKTIREMGKVENGLAV 60
           MDHSKPTLCVLDASSYVGFWVTKGLLNKGYPVHAAVQNTGKPEIEKTIREMG+VENGLAV
Sbjct: 1   MDHSKPTLCVLDASSYVGFWVTKGLLNKGYPVHAAVQNTGKPEIEKTIREMGEVENGLAV 60

Query: 61  FSVDIMDYQSILVALKGCSGLFCCMDTHHVYDEKMVDLEVRGTINVVEACAQTDSVEKIV 120
           FSVDIMDYQSILVALKGCSGLFCCMDTHHVYDEKMVDLEVRGTINVVEACAQTDSVEKIV
Sbjct: 61  FSVDIMDYQSILVALKGCSGLFCCMDTHHVYDEKMVDLEVRGTINVVEACAQTDSVEKIV 120

Query: 121 FTSSLTAAVWRENILSEKDVDERCWSDKEFCRKMKLWYPLAKTLSEQAAWALAMDRRLKM 180
           FTSSLTAAVWRENILSEKDVDERCWSDKEFCRKMKLWYPLAKTLSEQAAWALAMDRRLKM
Sbjct: 121 FTSSLTAAVWRENILSEKDVDERCWSDKEFCRKMKLWYPLAKTLSEQAAWALAMDRRLKM 180

Query: 181 VSINAGLVLGPAVAEENSGSTISYLKGAAQMYENGVLAVVDVKFLVDVHIRAMEDSSTGG 240
           VSINAGLVLGPAVAEENSGSTISYLKGAAQMYENGVLAVVDVKFLVDVHIRAMEDSSTGG
Sbjct: 181 VSINAGLVLGPAVAEENSGSTISYLKGAAQMYENGVLAVVDVKFLVDVHIRAMEDSSTGG 240

Query: 241 RYFCFDQIVNSEEETVKLANILRPLISLPQRYEVQGREAFAERLRTRKLHKLIEGTA 298
           RYFCFDQIVNSEEETVKLANILRPLIS+PQRYEVQGREAFAERLRTRKLHKLIEGTA
Sbjct: 241 RYFCFDQIVNSEEETVKLANILRPLISIPQRYEVQGREAFAERLRTRKLHKLIEGTA 297

BLAST of CSPI01G18070 vs. ExPASy TrEMBL
Match: A0A1S3B8Z2 (cinnamoyl-CoA reductase 1 isoform X1 OS=Cucumis melo OX=3656 GN=LOC103487321 PE=4 SV=1)

HSP 1 Score: 583.6 bits (1503), Expect = 4.8e-163
Identity = 291/297 (97.98%), Postives = 293/297 (98.65%), Query Frame = 0

Query: 1   MDHSKPTLCVLDASSYVGFWVTKGLLNKGYPVHAAVQNTGKPEIEKTIREMGKVENGLAV 60
           MD SKP LCVLDASSYVGFWVTKGLL+KGYPVHAAVQNTGKPEIEKTIREM KVENGL V
Sbjct: 1   MDPSKPILCVLDASSYVGFWVTKGLLSKGYPVHAAVQNTGKPEIEKTIREMCKVENGLMV 60

Query: 61  FSVDIMDYQSILVALKGCSGLFCCMDTHHVYDEKMVDLEVRGTINVVEACAQTDSVEKIV 120
           FSVDIMDYQSILVALKGCSGLFCCMDTHHVYDEKMVDLEVRGTINVVEACAQTDSVEKIV
Sbjct: 61  FSVDIMDYQSILVALKGCSGLFCCMDTHHVYDEKMVDLEVRGTINVVEACAQTDSVEKIV 120

Query: 121 FTSSLTAAVWRENILSEKDVDERCWSDKEFCRKMKLWYPLAKTLSEQAAWALAMDRRLKM 180
           FTSSLTAAVWRENILSEKDVDERCWSDKEFCRKMKLWYPLAKTLSEQAAWALAMDRRLKM
Sbjct: 121 FTSSLTAAVWRENILSEKDVDERCWSDKEFCRKMKLWYPLAKTLSEQAAWALAMDRRLKM 180

Query: 181 VSINAGLVLGPAVAEENSGSTISYLKGAAQMYENGVLAVVDVKFLVDVHIRAMEDSSTGG 240
           VSINAGLVLGPAVAEENSGSTISYLKGAAQMYENGVLAVVDVKFLVDVHIRAMEDSSTGG
Sbjct: 181 VSINAGLVLGPAVAEENSGSTISYLKGAAQMYENGVLAVVDVKFLVDVHIRAMEDSSTGG 240

Query: 241 RYFCFDQIVNSEEETVKLANILRPLISLPQRYEVQGREAFAERLRTRKLHKLIEGTA 298
           RYFCFDQIVNSEEETVKLANILRPLISLPQRYEVQG+EAFAERLRTRKLHKLIEGTA
Sbjct: 241 RYFCFDQIVNSEEETVKLANILRPLISLPQRYEVQGKEAFAERLRTRKLHKLIEGTA 297

BLAST of CSPI01G18070 vs. ExPASy TrEMBL
Match: A0A6J1JM00 (cinnamoyl-CoA reductase-like SNL6 OS=Cucurbita maxima OX=3661 GN=LOC111485788 PE=3 SV=1)

HSP 1 Score: 560.1 bits (1442), Expect = 5.7e-156
Identity = 274/297 (92.26%), Postives = 286/297 (96.30%), Query Frame = 0

Query: 1   MDHSKPTLCVLDASSYVGFWVTKGLLNKGYPVHAAVQNTGKPEIEKTIREMGKVENGLAV 60
           M+H K TLCVLDASSYVGFW+TKGLLN+GYPVHAAV+ TG PEIEK IREMGKVENGL V
Sbjct: 1   MEHRKLTLCVLDASSYVGFWITKGLLNRGYPVHAAVRKTGNPEIEKAIREMGKVENGLVV 60

Query: 61  FSVDIMDYQSILVALKGCSGLFCCMDTHHVYDEKMVDLEVRGTINVVEACAQTDSVEKIV 120
           F VDIMDY SIL+ALKGCSGLFCCMDT HVYDEKMV+LEVRGTINVVEACA+TD+V K V
Sbjct: 61  FPVDIMDYHSILIALKGCSGLFCCMDTPHVYDEKMVELEVRGTINVVEACARTDTVHKTV 120

Query: 121 FTSSLTAAVWRENILSEKDVDERCWSDKEFCRKMKLWYPLAKTLSEQAAWALAMDRRLKM 180
           FTSSLTAAVWRENILSEKDVDERCWSDKEFCRKMKLWYPLAKTLSEQAAWALAMDRRLKM
Sbjct: 121 FTSSLTAAVWRENILSEKDVDERCWSDKEFCRKMKLWYPLAKTLSEQAAWALAMDRRLKM 180

Query: 181 VSINAGLVLGPAVAEENSGSTISYLKGAAQMYENGVLAVVDVKFLVDVHIRAMEDSSTGG 240
           VSINAGLVLGPAVAE+NSGSTISYLKGAAQMYENGVLAVVDVKFLVDVHIRAMEDSSTGG
Sbjct: 181 VSINAGLVLGPAVAEQNSGSTISYLKGAAQMYENGVLAVVDVKFLVDVHIRAMEDSSTGG 240

Query: 241 RYFCFDQIVNSEEETVKLANILRPLISLPQRYEVQGREAFAERLRTRKLHKLIEGTA 298
           RYFCFDQIVNSEEET+KLANILRPLISLPQRYEVQGREAFAERLRTR+LHKL+EGTA
Sbjct: 241 RYFCFDQIVNSEEETLKLANILRPLISLPQRYEVQGREAFAERLRTRRLHKLVEGTA 297

BLAST of CSPI01G18070 vs. ExPASy TrEMBL
Match: A0A6J1E516 (cinnamoyl-CoA reductase-like SNL6 OS=Cucurbita moschata OX=3662 GN=LOC111430012 PE=3 SV=1)

HSP 1 Score: 557.0 bits (1434), Expect = 4.8e-155
Identity = 272/297 (91.58%), Postives = 285/297 (95.96%), Query Frame = 0

Query: 1   MDHSKPTLCVLDASSYVGFWVTKGLLNKGYPVHAAVQNTGKPEIEKTIREMGKVENGLAV 60
           M+H KPTLCVLDASSYVGFW+TK LLN+GYPVHAAV+ TG PE+EKTIRE G+VENGL V
Sbjct: 12  MEHRKPTLCVLDASSYVGFWITKELLNRGYPVHAAVRKTGNPEMEKTIRETGEVENGLVV 71

Query: 61  FSVDIMDYQSILVALKGCSGLFCCMDTHHVYDEKMVDLEVRGTINVVEACAQTDSVEKIV 120
           F VDIMDY SIL ALKGCSGLFCCMDT HVYDEKMV+LEVRGTINVVEACAQTD+V K V
Sbjct: 72  FPVDIMDYHSILAALKGCSGLFCCMDTPHVYDEKMVELEVRGTINVVEACAQTDTVHKTV 131

Query: 121 FTSSLTAAVWRENILSEKDVDERCWSDKEFCRKMKLWYPLAKTLSEQAAWALAMDRRLKM 180
           +TSSLTAAVWRENILSEKDVDERCWSDKEFCRKMKLWYPLAKTLSEQAAWALAMDRRLKM
Sbjct: 132 YTSSLTAAVWRENILSEKDVDERCWSDKEFCRKMKLWYPLAKTLSEQAAWALAMDRRLKM 191

Query: 181 VSINAGLVLGPAVAEENSGSTISYLKGAAQMYENGVLAVVDVKFLVDVHIRAMEDSSTGG 240
           VSINAGLVLGPAVAE+NSGSTISYLKGAAQMYENGVLAVVDVKFLVDVHIRAMEDSSTGG
Sbjct: 192 VSINAGLVLGPAVAEQNSGSTISYLKGAAQMYENGVLAVVDVKFLVDVHIRAMEDSSTGG 251

Query: 241 RYFCFDQIVNSEEETVKLANILRPLISLPQRYEVQGREAFAERLRTRKLHKLIEGTA 298
           RYFCFDQIVNSEEET+KLANILRPLISLPQRYEVQGREAFAERLRTR+LHKL+EGTA
Sbjct: 252 RYFCFDQIVNSEEETLKLANILRPLISLPQRYEVQGREAFAERLRTRRLHKLVEGTA 308

BLAST of CSPI01G18070 vs. ExPASy TrEMBL
Match: A0A6J1KKL4 (cinnamoyl-CoA reductase-like SNL6 isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111495416 PE=4 SV=1)

HSP 1 Score: 549.7 bits (1415), Expect = 7.7e-153
Identity = 270/297 (90.91%), Postives = 282/297 (94.95%), Query Frame = 0

Query: 1   MDHSKPTLCVLDASSYVGFWVTKGLLNKGYPVHAAVQNTGKPEIEKTIREMGKVENGLAV 60
           MDH KPTLCVLDASSYVG WVTKGL+N+GYPVHAA+Q TG PEIEKTIREMGKVENGL V
Sbjct: 1   MDHRKPTLCVLDASSYVGLWVTKGLINRGYPVHAAIQKTGNPEIEKTIREMGKVENGLVV 60

Query: 61  FSVDIMDYQSILVALKGCSGLFCCMDTHHVYDEKMVDLEVRGTINVVEACAQTDSVEKIV 120
           F VDIMDYQSILVAL+GCSGLFCCMDT HVYDEKMVDLEVRGTINVVEACA+TD+V+KIV
Sbjct: 61  FPVDIMDYQSILVALEGCSGLFCCMDTPHVYDEKMVDLEVRGTINVVEACARTDTVDKIV 120

Query: 121 FTSSLTAAVWRENILSEKDVDERCWSDKEFCRKMKLWYPLAKTLSEQAAWALAMDRRLKM 180
           FTSSLTAAVWRENILSEKDVDERCWSDKEFCRKMKLWYPLAKTLSEQAAWALAMDRRL M
Sbjct: 121 FTSSLTAAVWRENILSEKDVDERCWSDKEFCRKMKLWYPLAKTLSEQAAWALAMDRRLNM 180

Query: 181 VSINAGLVLGPAVAEENSGSTISYLKGAAQMYENGVLAVVDVKFLVDVHIRAMEDSSTGG 240
           VSINAGLVLGPAVAEENS STISYLKGAA+MYENGVLAVVDVKFLVDVHIRAMEDSSTGG
Sbjct: 181 VSINAGLVLGPAVAEENSVSTISYLKGAAEMYENGVLAVVDVKFLVDVHIRAMEDSSTGG 240

Query: 241 RYFCFDQIVNSEEETVKLANILRPLISLPQRYEVQGREAFAERLRTRKLHKLIEGTA 298
           RYFCFDQIVNSE+ETVKLAN LRPLIS+P R E QGRE FAERLR+R+LHKLIEG A
Sbjct: 241 RYFCFDQIVNSEDETVKLANTLRPLISIPPRCEGQGREGFAERLRSRRLHKLIEGAA 297

BLAST of CSPI01G18070 vs. NCBI nr
Match: XP_011656009.1 (cinnamoyl-CoA reductase-like SNL6 [Cucumis sativus] >KGN65259.1 hypothetical protein Csa_019971 [Cucumis sativus] >QHJ91598.1 cinnamoyl-CoA reductase 1 [Cucumis sativus])

HSP 1 Score: 595.9 bits (1535), Expect = 1.9e-166
Identity = 295/297 (99.33%), Postives = 297/297 (100.00%), Query Frame = 0

Query: 1   MDHSKPTLCVLDASSYVGFWVTKGLLNKGYPVHAAVQNTGKPEIEKTIREMGKVENGLAV 60
           MDHSKPTLCVLDASSYVGFWVTKGLLNKGYPVHAAVQNTGKPEIEKTIREMG+VENGLAV
Sbjct: 1   MDHSKPTLCVLDASSYVGFWVTKGLLNKGYPVHAAVQNTGKPEIEKTIREMGEVENGLAV 60

Query: 61  FSVDIMDYQSILVALKGCSGLFCCMDTHHVYDEKMVDLEVRGTINVVEACAQTDSVEKIV 120
           FSVDIMDYQSILVALKGCSGLFCCMDTHHVYDEKMVDLEVRGTINVVEACAQTDSVEKIV
Sbjct: 61  FSVDIMDYQSILVALKGCSGLFCCMDTHHVYDEKMVDLEVRGTINVVEACAQTDSVEKIV 120

Query: 121 FTSSLTAAVWRENILSEKDVDERCWSDKEFCRKMKLWYPLAKTLSEQAAWALAMDRRLKM 180
           FTSSLTAAVWRENILSEKDVDERCWSDKEFCRKMKLWYPLAKTLSEQAAWALAMDRRLKM
Sbjct: 121 FTSSLTAAVWRENILSEKDVDERCWSDKEFCRKMKLWYPLAKTLSEQAAWALAMDRRLKM 180

Query: 181 VSINAGLVLGPAVAEENSGSTISYLKGAAQMYENGVLAVVDVKFLVDVHIRAMEDSSTGG 240
           VSINAGLVLGPAVAEENSGSTISYLKGAAQMYENGVLAVVDVKFLVDVHIRAMEDSSTGG
Sbjct: 181 VSINAGLVLGPAVAEENSGSTISYLKGAAQMYENGVLAVVDVKFLVDVHIRAMEDSSTGG 240

Query: 241 RYFCFDQIVNSEEETVKLANILRPLISLPQRYEVQGREAFAERLRTRKLHKLIEGTA 298
           RYFCFDQIVNSEEETVKLANILRPLIS+PQRYEVQGREAFAERLRTRKLHKLIEGTA
Sbjct: 241 RYFCFDQIVNSEEETVKLANILRPLISIPQRYEVQGREAFAERLRTRKLHKLIEGTA 297

BLAST of CSPI01G18070 vs. NCBI nr
Match: XP_008443817.1 (PREDICTED: cinnamoyl-CoA reductase 1 isoform X1 [Cucumis melo])

HSP 1 Score: 583.6 bits (1503), Expect = 9.9e-163
Identity = 291/297 (97.98%), Postives = 293/297 (98.65%), Query Frame = 0

Query: 1   MDHSKPTLCVLDASSYVGFWVTKGLLNKGYPVHAAVQNTGKPEIEKTIREMGKVENGLAV 60
           MD SKP LCVLDASSYVGFWVTKGLL+KGYPVHAAVQNTGKPEIEKTIREM KVENGL V
Sbjct: 1   MDPSKPILCVLDASSYVGFWVTKGLLSKGYPVHAAVQNTGKPEIEKTIREMCKVENGLMV 60

Query: 61  FSVDIMDYQSILVALKGCSGLFCCMDTHHVYDEKMVDLEVRGTINVVEACAQTDSVEKIV 120
           FSVDIMDYQSILVALKGCSGLFCCMDTHHVYDEKMVDLEVRGTINVVEACAQTDSVEKIV
Sbjct: 61  FSVDIMDYQSILVALKGCSGLFCCMDTHHVYDEKMVDLEVRGTINVVEACAQTDSVEKIV 120

Query: 121 FTSSLTAAVWRENILSEKDVDERCWSDKEFCRKMKLWYPLAKTLSEQAAWALAMDRRLKM 180
           FTSSLTAAVWRENILSEKDVDERCWSDKEFCRKMKLWYPLAKTLSEQAAWALAMDRRLKM
Sbjct: 121 FTSSLTAAVWRENILSEKDVDERCWSDKEFCRKMKLWYPLAKTLSEQAAWALAMDRRLKM 180

Query: 181 VSINAGLVLGPAVAEENSGSTISYLKGAAQMYENGVLAVVDVKFLVDVHIRAMEDSSTGG 240
           VSINAGLVLGPAVAEENSGSTISYLKGAAQMYENGVLAVVDVKFLVDVHIRAMEDSSTGG
Sbjct: 181 VSINAGLVLGPAVAEENSGSTISYLKGAAQMYENGVLAVVDVKFLVDVHIRAMEDSSTGG 240

Query: 241 RYFCFDQIVNSEEETVKLANILRPLISLPQRYEVQGREAFAERLRTRKLHKLIEGTA 298
           RYFCFDQIVNSEEETVKLANILRPLISLPQRYEVQG+EAFAERLRTRKLHKLIEGTA
Sbjct: 241 RYFCFDQIVNSEEETVKLANILRPLISLPQRYEVQGKEAFAERLRTRKLHKLIEGTA 297

BLAST of CSPI01G18070 vs. NCBI nr
Match: XP_038879610.1 (cinnamoyl-CoA reductase-like SNL6 isoform X1 [Benincasa hispida])

HSP 1 Score: 570.1 bits (1468), Expect = 1.1e-158
Identity = 281/297 (94.61%), Postives = 290/297 (97.64%), Query Frame = 0

Query: 1   MDHSKPTLCVLDASSYVGFWVTKGLLNKGYPVHAAVQNTGKPEIEKTIREMGKVENGLAV 60
           MDH+KP LCVLDASSYVGFWVTK LLNKGYPVHAAVQN GKPEIE+TIREMGK++NGL  
Sbjct: 1   MDHTKPILCVLDASSYVGFWVTKELLNKGYPVHAAVQNNGKPEIERTIREMGKMKNGLVF 60

Query: 61  FSVDIMDYQSILVALKGCSGLFCCMDTHHVYDEKMVDLEVRGTINVVEACAQTDSVEKIV 120
           FSVDIMDYQSILVALKGCSGLFCCMDT HV+DEKMVDLEVRGTINVVEACAQTD+V KIV
Sbjct: 61  FSVDIMDYQSILVALKGCSGLFCCMDTPHVFDEKMVDLEVRGTINVVEACAQTDTVNKIV 120

Query: 121 FTSSLTAAVWRENILSEKDVDERCWSDKEFCRKMKLWYPLAKTLSEQAAWALAMDRRLKM 180
           FTSSLTAAVWRENILSEKDVDERCWSDKEFCRKMKLWYPLAKTLSEQAAWALA+DR+LKM
Sbjct: 121 FTSSLTAAVWRENILSEKDVDERCWSDKEFCRKMKLWYPLAKTLSEQAAWALAVDRKLKM 180

Query: 181 VSINAGLVLGPAVAEENSGSTISYLKGAAQMYENGVLAVVDVKFLVDVHIRAMEDSSTGG 240
           VSINAGLVLGPAVAEENSGSTISYLKGAAQMYENGVLAVVDVKFLVDVHIRAMEDSSTGG
Sbjct: 181 VSINAGLVLGPAVAEENSGSTISYLKGAAQMYENGVLAVVDVKFLVDVHIRAMEDSSTGG 240

Query: 241 RYFCFDQIVNSEEETVKLANILRPLISLPQRYEVQGREAFAERLRTRKLHKLIEGTA 298
           RYFCFDQIVNSEEETVKLANILRPLISLPQRYEVQGREAFAERLRTR+LHKLIEGTA
Sbjct: 241 RYFCFDQIVNSEEETVKLANILRPLISLPQRYEVQGREAFAERLRTRRLHKLIEGTA 297

BLAST of CSPI01G18070 vs. NCBI nr
Match: KAG6589749.1 (Cinnamoyl-CoA reductase-like SNL6, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 560.8 bits (1444), Expect = 6.9e-156
Identity = 274/297 (92.26%), Postives = 287/297 (96.63%), Query Frame = 0

Query: 1   MDHSKPTLCVLDASSYVGFWVTKGLLNKGYPVHAAVQNTGKPEIEKTIREMGKVENGLAV 60
           M+H KPTLCVLDASSYVGFW+TK LLN+GYPVHAAV+ TG PE+EKTIREMG+VENGL V
Sbjct: 1   MEHRKPTLCVLDASSYVGFWITKELLNRGYPVHAAVRKTGNPEMEKTIREMGEVENGLVV 60

Query: 61  FSVDIMDYQSILVALKGCSGLFCCMDTHHVYDEKMVDLEVRGTINVVEACAQTDSVEKIV 120
           F VDIMDY SILVALKGCSGLFCCMDT HVYDEKMV+LEVRGTINVVEACAQTD+V K V
Sbjct: 61  FPVDIMDYHSILVALKGCSGLFCCMDTPHVYDEKMVELEVRGTINVVEACAQTDTVHKTV 120

Query: 121 FTSSLTAAVWRENILSEKDVDERCWSDKEFCRKMKLWYPLAKTLSEQAAWALAMDRRLKM 180
           +TSSLTAAVWRENILSEKDVDERCWSDKEFCRKMKLWYPLAKTLSEQAAWALAMDRRLKM
Sbjct: 121 YTSSLTAAVWRENILSEKDVDERCWSDKEFCRKMKLWYPLAKTLSEQAAWALAMDRRLKM 180

Query: 181 VSINAGLVLGPAVAEENSGSTISYLKGAAQMYENGVLAVVDVKFLVDVHIRAMEDSSTGG 240
           VSINAGLVLGPAVAE+NSGSTISYLKGAAQMYENGVLAVVDVKFLVDVHIRAMEDSSTGG
Sbjct: 181 VSINAGLVLGPAVAEQNSGSTISYLKGAAQMYENGVLAVVDVKFLVDVHIRAMEDSSTGG 240

Query: 241 RYFCFDQIVNSEEETVKLANILRPLISLPQRYEVQGREAFAERLRTRKLHKLIEGTA 298
           RYFCFDQIVNSEEET+KLANILRPLISLPQRYEVQGREAFAERLRTR+LHKL+EGTA
Sbjct: 241 RYFCFDQIVNSEEETLKLANILRPLISLPQRYEVQGREAFAERLRTRRLHKLVEGTA 297

BLAST of CSPI01G18070 vs. NCBI nr
Match: XP_022988589.1 (cinnamoyl-CoA reductase-like SNL6 [Cucurbita maxima])

HSP 1 Score: 560.1 bits (1442), Expect = 1.2e-155
Identity = 274/297 (92.26%), Postives = 286/297 (96.30%), Query Frame = 0

Query: 1   MDHSKPTLCVLDASSYVGFWVTKGLLNKGYPVHAAVQNTGKPEIEKTIREMGKVENGLAV 60
           M+H K TLCVLDASSYVGFW+TKGLLN+GYPVHAAV+ TG PEIEK IREMGKVENGL V
Sbjct: 1   MEHRKLTLCVLDASSYVGFWITKGLLNRGYPVHAAVRKTGNPEIEKAIREMGKVENGLVV 60

Query: 61  FSVDIMDYQSILVALKGCSGLFCCMDTHHVYDEKMVDLEVRGTINVVEACAQTDSVEKIV 120
           F VDIMDY SIL+ALKGCSGLFCCMDT HVYDEKMV+LEVRGTINVVEACA+TD+V K V
Sbjct: 61  FPVDIMDYHSILIALKGCSGLFCCMDTPHVYDEKMVELEVRGTINVVEACARTDTVHKTV 120

Query: 121 FTSSLTAAVWRENILSEKDVDERCWSDKEFCRKMKLWYPLAKTLSEQAAWALAMDRRLKM 180
           FTSSLTAAVWRENILSEKDVDERCWSDKEFCRKMKLWYPLAKTLSEQAAWALAMDRRLKM
Sbjct: 121 FTSSLTAAVWRENILSEKDVDERCWSDKEFCRKMKLWYPLAKTLSEQAAWALAMDRRLKM 180

Query: 181 VSINAGLVLGPAVAEENSGSTISYLKGAAQMYENGVLAVVDVKFLVDVHIRAMEDSSTGG 240
           VSINAGLVLGPAVAE+NSGSTISYLKGAAQMYENGVLAVVDVKFLVDVHIRAMEDSSTGG
Sbjct: 181 VSINAGLVLGPAVAEQNSGSTISYLKGAAQMYENGVLAVVDVKFLVDVHIRAMEDSSTGG 240

Query: 241 RYFCFDQIVNSEEETVKLANILRPLISLPQRYEVQGREAFAERLRTRKLHKLIEGTA 298
           RYFCFDQIVNSEEET+KLANILRPLISLPQRYEVQGREAFAERLRTR+LHKL+EGTA
Sbjct: 241 RYFCFDQIVNSEEETLKLANILRPLISLPQRYEVQGREAFAERLRTRRLHKLVEGTA 297

BLAST of CSPI01G18070 vs. TAIR 10
Match: AT4G30470.1 (NAD(P)-binding Rossmann-fold superfamily protein )

HSP 1 Score: 404.1 bits (1037), Expect = 1.0e-112
Identity = 197/298 (66.11%), Postives = 239/298 (80.20%), Query Frame = 0

Query: 1   MDHSKPT--LCVLDASSYVGFWVTKGLLNKGYPVHAAVQNTGKPEIEKTIREMGKVENGL 60
           MDH K T   CVLDAS+YVGFW+ K LL++GY VHAA++  G+ EIE+ IREM   E  L
Sbjct: 1   MDHDKSTSCCCVLDASTYVGFWILKKLLSRGYSVHAAIRRNGESEIEEMIREMETTEERL 60

Query: 61  AVFSVDIMDYQSILVALKGCSGLFCCMDTHHVYDEKMVDLEVRGTINVVEACAQTDSVEK 120
            V+ VD++DYQSILV+LK C+ +FCC+D+   YDEK VDLEVRG INVVEAC +T+S+EK
Sbjct: 61  VVYDVDVLDYQSILVSLKTCNVVFCCLDSPEGYDEKEVDLEVRGAINVVEACGRTESIEK 120

Query: 121 IVFTSSLTAAVWRENILSEKDVDERCWSDKEFCRKMKLWYPLAKTLSEQAAWALAMDRRL 180
           IVF+SSLTA++WR+NI ++KDVDE+CWSD++FCR  KLW+ LAK LSE+AAWALAMDRRL
Sbjct: 121 IVFSSSLTASIWRDNIGTQKDVDEKCWSDQDFCRSKKLWHALAKMLSEKAAWALAMDRRL 180

Query: 181 KMVSINAGLVLGPAVAEENSGSTISYLKGAAQMYENGVLAVVDVKFLVDVHIRAMEDSST 240
            MVSIN GLV+GP+VA+ N+  T+SYLKGAAQMYENGVLA VDVKFL DVHIRA ED S 
Sbjct: 181 NMVSINPGLVVGPSVAQHNARPTMSYLKGAAQMYENGVLAYVDVKFLADVHIRAFEDVSA 240

Query: 241 GGRYFCFDQIVNSEEETVKLANILRPLISLPQRY--EVQGREAFAERLRTRKLHKLIE 295
            GRYFCF+QIVN+EEE +KL   L PLI +P RY  E+ G E + ERLR  KL KL+E
Sbjct: 241 CGRYFCFNQIVNTEEEALKLVESLSPLIPMPPRYENEMHGSEVYEERLRNNKLSKLVE 298

BLAST of CSPI01G18070 vs. TAIR 10
Match: AT2G23910.1 (NAD(P)-binding Rossmann-fold superfamily protein )

HSP 1 Score: 381.7 bits (979), Expect = 5.3e-106
Identity = 183/289 (63.32%), Postives = 232/289 (80.28%), Query Frame = 0

Query: 9   CVLDASSYVGFWVTKGLLNKGYPVHAAVQNTGKPEIEKTIREMGKVENGLAVFSVDIMDY 68
           CVLDAS+YVGFW+ K LL +GY VHAA++  G+  +E+ IR+M   E  L V+ VD++DY
Sbjct: 11  CVLDASTYVGFWILKRLLTRGYSVHAAIRKNGESVLEEKIRDMEANEERLEVYDVDVLDY 70

Query: 69  QSILVALKGCSGLFCCMDTHHVYD-EKMVDLEVRGTINVVEACAQTDSVEKIVFTSSLTA 128
           QSIL++L  C+ +FCC+D    YD EK VDLEVRG INVVEACA+T+S+EKIVF+SSLTA
Sbjct: 71  QSILISLNNCNAVFCCLDNPEGYDEEKEVDLEVRGAINVVEACARTESIEKIVFSSSLTA 130

Query: 129 AVWRENILSEKDVDERCWSDKEFCRKMKLWYPLAKTLSEQAAWALAMDRRLKMVSINAGL 188
           A+WR+NI ++KDVDE+CWSD +FC K KLW+ LAKT SE+AAWALAMDR + MVS+N GL
Sbjct: 131 AIWRDNIGTQKDVDEKCWSDLDFCLKKKLWHALAKTQSEKAAWALAMDRMVNMVSVNPGL 190

Query: 189 VLGPAVAEENSGSTISYLKGAAQMYENGVLAVVDVKFLVDVHIRAMEDSSTGGRYFCFDQ 248
           ++GP+VA+ N   T+SYLKGAAQMYENGVLA VDV+F+ DVHIRA ED+S  GRYFCF+Q
Sbjct: 191 IVGPSVAQHNPRPTMSYLKGAAQMYENGVLAYVDVEFVADVHIRAFEDTSACGRYFCFNQ 250

Query: 249 IVNSEEETVKLANILRPLISLPQRY--EVQGREAFAERLRTRKLHKLIE 295
           IVN+EEE +KL   L PLI +P R+  E+QG E + ERLR +KL+KL+E
Sbjct: 251 IVNTEEEALKLVQTLSPLIPMPPRHEKEMQGSEVYEERLRNKKLNKLVE 299

BLAST of CSPI01G18070 vs. TAIR 10
Match: AT5G58490.1 (NAD(P)-binding Rossmann-fold superfamily protein )

HSP 1 Score: 166.0 bits (419), Expect = 4.6e-41
Identity = 92/274 (33.58%), Postives = 154/274 (56.20%), Query Frame = 0

Query: 5   KPTLCVLDASSYVGFWVTKGLLNKGYPVHAAVQNTGKPEIEKTIREMGKVENGLAVFSVD 64
           +  +CV  AS  +G W+   LL +GY VHA V+N    +  K +  +      L +F +D
Sbjct: 6   REVVCVTGASGCIGSWLVHQLLLRGYSVHATVKNLQDEKETKHLEGLEGAATRLHLFEMD 65

Query: 65  IMDYQSILVALKGCSGLF-----CCMDTHHVYDEKMVDLEVRGTINVVEACAQTDSVEKI 124
           ++ Y ++  A+ GCSG+F     C +D      ++++D  V+GTINV+ A A+  SV+++
Sbjct: 66  LLQYDTVSAAINGCSGVFHLASPCIVDEVQDPQKQLLDPAVKGTINVLTA-AKEASVKRV 125

Query: 125 VFTSSLTAAVWRENILSEKDVDERCWSDKEFCRKMKLWYPLAKTLSEQAAWALAMDRRLK 184
           V TSS++A     N  ++K  +E CW+ +++CR+  LWYPL+KTL+E+AAW  A ++ L 
Sbjct: 126 VVTSSISAITPSPNWPADKIKNEECWAAEDYCRQNGLWYPLSKTLAEKAAWEFAEEKGLD 185

Query: 185 MVSINAGLVLGPAVAEENSGS---TISYLKGAAQMYENGVLAVVDVKFLVDVHIRAMEDS 244
           +V +N G V+GP +    + S    +  L+G  + YEN  +  V  K +   HI   ED 
Sbjct: 186 VVVVNPGTVMGPVIPPSLNASMHMLLRLLQGCTETYENFFMGSVHFKDVALAHILVYEDP 245

Query: 245 STGGRYFCFDQIVNSEEETVKLANILRPLISLPQ 271
            + GR+ C + I +  +   K+A  L P  ++P+
Sbjct: 246 YSKGRHLCVEAISHYGDFVAKVAE-LYPNYNVPK 277

BLAST of CSPI01G18070 vs. TAIR 10
Match: AT1G51410.1 (NAD(P)-binding Rossmann-fold superfamily protein )

HSP 1 Score: 164.5 bits (415), Expect = 1.3e-40
Identity = 96/273 (35.16%), Postives = 154/273 (56.41%), Query Frame = 0

Query: 7   TLCVLDASSYVGFWVTKGLLNKGYPVHAAVQNTGKPEIEKTIREMGKVENGLAVFSVDIM 66
           T+CV  AS Y+  W+ K LL +GY V A+V++   P   + +  +   E  L +F  +++
Sbjct: 8   TVCVTGASGYIASWIVKLLLLRGYTVKASVRDPNDPRKTEHLLALEGAEERLKLFKANLL 67

Query: 67  DYQSILVALKGCSGLFCCMDT--HHVYD--EKMVDLEVRGTINVVEACAQTDSVEKIVFT 126
           +  S   A+ GC G+F       H V D   +++D  V+GTINV+ +C +T SV+++V T
Sbjct: 68  EEGSFDSAIDGCEGVFHTASPFYHDVKDPQAELLDPAVKGTINVLSSCLKTSSVKRVVLT 127

Query: 127 SSLTAAVWRENILS-EKDVDERCWSDKEFCRKMKLWYPLAKTLSEQAAWALAMDRRLKMV 186
           SS+ A  +     + E  VDE  ++D ++CR  KLWY L+KTL+E AAW  A +  L++V
Sbjct: 128 SSIAAVAFNGMPRTPETIVDETWFADPDYCRASKLWYVLSKTLAENAAWKFAKENNLQLV 187

Query: 187 SINAGLVLGPAV---AEENSGSTISYLKGAAQMYENGVLAVVDVKFLVDVHIRAMEDSST 246
           SIN  +V+GP +      ++ + +S +KG AQ + N     V+VK + + HI+A E+   
Sbjct: 188 SINPAMVIGPLLQPTLNTSAAAVLSLIKG-AQTFPNATFGWVNVKDVANAHIQAFENPDA 247

Query: 247 GGRYFCFDQIVNSEEETVKLANILRPLISLPQR 272
            GRY C  + V    E V + + L P   LP++
Sbjct: 248 DGRY-CLVERVAHYSEVVNILHDLYPDFQLPEK 278

BLAST of CSPI01G18070 vs. TAIR 10
Match: AT5G14700.1 (NAD(P)-binding Rossmann-fold superfamily protein )

HSP 1 Score: 163.3 bits (412), Expect = 3.0e-40
Identity = 100/275 (36.36%), Postives = 146/275 (53.09%), Query Frame = 0

Query: 2   DHSKPTLCVLDASSYVGFWVTKGLLNKGYPVHAAVQNTGKPEIEKTIREM------GKVE 61
           D     +CV    SY+G  + K LL  GY V   V     PE ++ + EM          
Sbjct: 49  DAGNRLVCVTGGVSYLGRAIVKRLLVHGYSVRIVVD---CPEDKEKVSEMEADAETASFS 108

Query: 62  NGLAVFSVDIMDYQSILVALKGCSGLF---CCMDTHHV--YDEKMVDLEVRGTINVVEAC 121
           N +      + +  S++ A  GC+G+F     +D   V  Y + M +LE + + +V+EAC
Sbjct: 109 NMITSVVSRLTEIDSLIKAFDGCAGVFHTSAFVDPAGVSGYSKSMAELEAKVSESVIEAC 168

Query: 122 AQTDSVEKIVFTSSLTAAVWRENILSEKD---VDERCWSDKEFCRKMKLWYPLAKTLSEQ 181
            +T SV K VFTSSL A  W++N  +  D   ++E  WSD++ C   KLWY L K  +E+
Sbjct: 169 TRTASVRKCVFTSSLLACAWQKNPCNSLDHSVINEESWSDEQLCIDNKLWYALGKLKAEK 228

Query: 182 AAWALAMDRRLKMVSINAGLVLGPAVAEENSGSTISYLKGAAQMYENGVLAVVDVKFLVD 241
           AAW +A  + LK+ +I   L+ GP     NS ST++YLKGA +MY NG+LA +DV  L  
Sbjct: 229 AAWRIADSKGLKLATICPALITGPDFFNRNSTSTLAYLKGAKEMYSNGLLATMDVNRLAK 288

Query: 242 VHI---RAMEDSSTGGRYFCFDQIVNSEEETVKLA 260
            H+     + + +  GRY CFD I+ S +   KLA
Sbjct: 289 AHVCLWEGLGNKTAFGRYICFDTIL-SRDGAEKLA 319

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
A0A059TC022.5e-3935.42Cinnamoyl-CoA reductase 1 OS=Petunia hybrida OX=4102 GN=CCR1 PE=1 SV=1[more]
A0A0B6VQ481.8e-3731.88Phenylacetaldehyde reductase OS=Rosa hybrid cultivar OX=128735 GN=PAR PE=1 SV=1[more]
Q9S9N92.8e-3533.96Cinnamoyl-CoA reductase 1 OS=Arabidopsis thaliana OX=3702 GN=CCR1 PE=1 SV=1[more]
Q9SAH94.8e-3532.96Cinnamoyl-CoA reductase 2 OS=Arabidopsis thaliana OX=3702 GN=CCR2 PE=1 SV=1[more]
Q500U87.7e-3328.13Tetraketide alpha-pyrone reductase 1 OS=Arabidopsis thaliana OX=3702 GN=TKPR1 PE... [more]
Match NameE-valueIdentityDescription
A0A0A0LW909.3e-16799.33Cinnamoyl-CoA reductase 1 OS=Cucumis sativus OX=3659 GN=CCR1 PE=2 SV=1[more]
A0A1S3B8Z24.8e-16397.98cinnamoyl-CoA reductase 1 isoform X1 OS=Cucumis melo OX=3656 GN=LOC103487321 PE=... [more]
A0A6J1JM005.7e-15692.26cinnamoyl-CoA reductase-like SNL6 OS=Cucurbita maxima OX=3661 GN=LOC111485788 PE... [more]
A0A6J1E5164.8e-15591.58cinnamoyl-CoA reductase-like SNL6 OS=Cucurbita moschata OX=3662 GN=LOC111430012 ... [more]
A0A6J1KKL47.7e-15390.91cinnamoyl-CoA reductase-like SNL6 isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC1... [more]
Match NameE-valueIdentityDescription
XP_011656009.11.9e-16699.33cinnamoyl-CoA reductase-like SNL6 [Cucumis sativus] >KGN65259.1 hypothetical pro... [more]
XP_008443817.19.9e-16397.98PREDICTED: cinnamoyl-CoA reductase 1 isoform X1 [Cucumis melo][more]
XP_038879610.11.1e-15894.61cinnamoyl-CoA reductase-like SNL6 isoform X1 [Benincasa hispida][more]
KAG6589749.16.9e-15692.26Cinnamoyl-CoA reductase-like SNL6, partial [Cucurbita argyrosperma subsp. sorori... [more]
XP_022988589.11.2e-15592.26cinnamoyl-CoA reductase-like SNL6 [Cucurbita maxima][more]
Match NameE-valueIdentityDescription
AT4G30470.11.0e-11266.11NAD(P)-binding Rossmann-fold superfamily protein [more]
AT2G23910.15.3e-10663.32NAD(P)-binding Rossmann-fold superfamily protein [more]
AT5G58490.14.6e-4133.58NAD(P)-binding Rossmann-fold superfamily protein [more]
AT1G51410.11.3e-4035.16NAD(P)-binding Rossmann-fold superfamily protein [more]
AT5G14700.13.0e-4036.36NAD(P)-binding Rossmann-fold superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (PI 183967) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableGENE3D3.40.50.720coord: 1..295
e-value: 3.6E-65
score: 222.3
NoneNo IPR availablePANTHERPTHR10366NAD DEPENDENT EPIMERASE/DEHYDRATASEcoord: 3..295
NoneNo IPR availablePANTHERPTHR10366:SF390CINNAMOYL-COA REDUCTASE-LIKE PROTEINcoord: 3..295
NoneNo IPR availableCDDcd08958FR_SDR_ecoord: 9..292
e-value: 1.1668E-114
score: 330.691
IPR0022253-beta hydroxysteroid dehydrogenase/isomerasePFAMPF010733Beta_HSDcoord: 43..169
e-value: 1.3E-9
score: 37.5
IPR036291NAD(P)-binding domain superfamilySUPERFAMILY51735NAD(P)-binding Rossmann-fold domainscoord: 4..254

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CSPI01G18070.1CSPI01G18070.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006694 steroid biosynthetic process
molecular_function GO:0003854 3-beta-hydroxy-delta5-steroid dehydrogenase activity
molecular_function GO:0016616 oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor