CSPI01G12600 (gene) Cucumber (PI 183967) v1

Overview
NameCSPI01G12600
Typegene
OrganismCucumis sativus var. hardwickii cv. PI 183967 (Cucumber (PI 183967) v1)
DescriptionDNA-directed RNA polymerase subunit
LocationChr1: 8033135 .. 8034854 (-)
RNA-Seq ExpressionCSPI01G12600
SyntenyCSPI01G12600
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGAAAAAGCCCAAGGTTCAAGGCTGTGAGCCTTGGGGCTTCACAAAGGGTGCCTCCTTGTTTTTTAAGCCTCGCCATAAACCAGACTTGCTCCTTGAGCCTAGGTGGGCATGCTTGAAAAGGCTTTTTAAAACACCGAGTGCAATGGATAATTTTTAATATTCATAATTTTCTAACGTCTACAAGGTCCTTGGTAGCCTCTTACTTCCAGTAACTACAAAGCATAGGTTATAATGTATATATGTGTTATATCTTTTCTCAGAACGGGACTTCAAGCTGTTATGGGCACTGAGGGAGTTGACGGGTGTAACACAAAGAGTAATCACATTATTGAAGTGCAACAAGTACTTGGCATTGAAGCTGCAAGGAAATGTATTATTGAAGAGATAAAATATACCATGGAAAGTCATGGAATGAGCATAGACATACGTCATATGATGCTTTTAGGAGATTTGATGACATTCAGGGTAAGTAAGTTGTGTTGGGTTACAAAAAATTACATGTTGTGTATAGGTTGAGGCAATTAAATATTTTTGCTGTGTGACAGGGTGAAGTTCTTGGAATCACAAGATTTGGCATTCAAAAAATGGATAAAAGTGTACTGATGCTTGCTTCATTTGAGAAAACCGCGGATCATCTTTTTAATGCTTCTGTGAATGGAAGGGATGATAAAATTGAAGGAGTTAGTGAATGCGTAATCATGGGTATACCAATGCCGCTAGGCACTGGCATGTTAAAAGTTAGACAAAGGTATATCTTCTCCTACTGGAACAGAACTCAGTTCATTATTATTATTATTGAGCATTGATTTCATGGGCAGCATCTGAACGCTTTTCTTAATGCCTGGAGTGTGATTTAACATAGTTTATGAGCAATTAGCTGAGAGAATTCTGTGTTCAAATTCCTACAATGTTATTTACTCCCCACTAATATTGATTTATACTTGTTGAACCTTCTAAATATTTCAAGCCTACAAGTGAGGACAGTGTTAGATGATATAATATTAGATTTACATTCATCCCTCAGTTAAAGTTTTTTAGTCAATTCATGATTCAGGTTCCAACATTATGGTTGAGTATTAACATCAGTTTAAATAATTTTGCATTTTACAAACTTTTAGTTTACATATTTTGAAGCTAATTGAGCTTTGTTTTTAACAGAGTTTCTGTGCCGCAACAATTACCTTATGGACCACCTGCAATTCTATCTTGAGTTCATCGACGCCGAGTCTGATTTGGTCGAGTTGGATGGCCTTAGGTGTAGTGTATTTCCTATAATATATAATGCAGAAGTTGAAATGATTTTTTTTGTGCTCAGTTTCAAAGAGTTCCTTTATGATAGCTATCAGAATAACTAAACTCTTGTTATTTTGTTATTTTGCAACCAGGCATGGATGGCTTTCGGCTTTTACAAGTTGGGGTTTGTATATAGTTGCGAGTTTTAGTTTGTATTCTGCTTTTTGTCATAAAGCAAGCAAAGCGTTTACATTCTACCCTATCAAACTAATTGTGGTAGGGCAAAATTTTGACATTATTATCAGGATAGCAAACTTTCTACCTCAATAGAAAGAGTATATATTAGTTATTGTTGAGCCTTGTTTATATTAGTAGCAGTAAGTATGGTATTCATTATCGGGACATGTGTGAAACTGTTTGGGGAATTGCCAAAAATTGGATAGAAAAAGAGTTTTAGGATTGATTGTAAAAAAGTTGAGATTTAG

mRNA sequence

ATGAAAAAGCCCAAGGTTCAAGGCTGTGAGCCTTGGGGCTTCACAAAGGGTGCCTCCTTGTTTTTTAAGCCTCGCCATAAACCAGACTTGCTCCTTGAGCCTAGAACGGGACTTCAAGCTGTTATGGGCACTGAGGGAGTTGACGGGTGTAACACAAAGAGTAATCACATTATTGAAGTGCAACAAGTACTTGGCATTGAAGCTGCAAGGAAATGTATTATTGAAGAGATAAAATATACCATGGAAAGTCATGGAATGAGCATAGACATACGTCATATGATGCTTTTAGGAGATTTGATGACATTCAGGGGTGAAGTTCTTGGAATCACAAGATTTGGCATTCAAAAAATGGATAAAAGTGTACTGATGCTTGCTTCATTTGAGAAAACCGCGGATCATCTTTTTAATGCTTCTGTGAATGGAAGGGATGATAAAATTGAAGGAGTTAGTGAATGCGTAATCATGGGTATACCAATGCCGCTAGGCACTGGCATGTTAAAAGTTAGACAAAGAGTTTCTGTGCCGCAACAATTACCTTATGGACCACCTGCAATTCTATCTTGAGTTCATCGACGCCGAGTCTGATTTGGTCGAGTTGGATGGCCTTAGGTGTAGTGTATTTCCTATAATATATAATGCAGAAGTTGAAATGATTTTTTTTGTGCTCAGTTTCAAAGAGTTCCTTTATGATAGCTATCAGAATAACTAAACTCTTGTTATTTTGTTATTTTGCAACCAGGCATGGATGGCTTTCGGCTTTTACAAGTTGGGGTTTGTATATAGTTGCGAGTTTTAGTTTGTATTCTGCTTTTTGTCATAAAGCAAGCAAAGCGTTTACATTCTACCCTATCAAACTAATTGTGGTAGGGCAAAATTTTGACATTATTATCAGGATAGCAAACTTTCTACCTCAATAGAAAGAGTATATATTAGTTATTGTTGAGCCTTGTTTATATTAGTAGCAGTAAGTATGGTATTCATTATCGGGACATGTGTGAAACTGTTTGGGGAATTGCCAAAAATTGGATAGAAAAAGAGTTTTAGGATTGATTGTAAAAAAGTTGAGATTTAG

Coding sequence (CDS)

ATGAAAAAGCCCAAGGTTCAAGGCTGTGAGCCTTGGGGCTTCACAAAGGGTGCCTCCTTGTTTTTTAAGCCTCGCCATAAACCAGACTTGCTCCTTGAGCCTAGAACGGGACTTCAAGCTGTTATGGGCACTGAGGGAGTTGACGGGTGTAACACAAAGAGTAATCACATTATTGAAGTGCAACAAGTACTTGGCATTGAAGCTGCAAGGAAATGTATTATTGAAGAGATAAAATATACCATGGAAAGTCATGGAATGAGCATAGACATACGTCATATGATGCTTTTAGGAGATTTGATGACATTCAGGGGTGAAGTTCTTGGAATCACAAGATTTGGCATTCAAAAAATGGATAAAAGTGTACTGATGCTTGCTTCATTTGAGAAAACCGCGGATCATCTTTTTAATGCTTCTGTGAATGGAAGGGATGATAAAATTGAAGGAGTTAGTGAATGCGTAATCATGGGTATACCAATGCCGCTAGGCACTGGCATGTTAAAAGTTAGACAAAGAGTTTCTGTGCCGCAACAATTACCTTATGGACCACCTGCAATTCTATCTTGA

Protein sequence

MKKPKVQGCEPWGFTKGASLFFKPRHKPDLLLEPRTGLQAVMGTEGVDGCNTKSNHIIEVQQVLGIEAARKCIIEEIKYTMESHGMSIDIRHMMLLGDLMTFRGEVLGITRFGIQKMDKSVLMLASFEKTADHLFNASVNGRDDKIEGVSECVIMGIPMPLGTGMLKVRQRVSVPQQLPYGPPAILS*
Homology
[Close]
BLAST of CSPI01G12600 vs. ExPASy Swiss-Prot
Match: F4JXF9 (DNA-directed RNA polymerase III subunit 1 OS=Arabidopsis thaliana OX=3702 GN=NRPC1 PE=2 SV=1)

HSP 1 Score: 200.3 bits (508), Expect = 2.0e-50
Identity = 101/152 (66.45%), Postives = 120/152 (78.95%), Query Frame = 0 

Query: 36   TGLQAVMGTEGVDGCNTKSNHIIEVQQVLGIEAARKCIIEEIKYTMESHGMSIDIRHMML 95
            T L AVMGT G++G  T SN+++EV + LGIEAAR  II+EI   M +HGMSIDIRHMML
Sbjct: 1225 TNLLAVMGTPGINGRTTTSNNVVEVSKTLGIEAARTTIIDEIGTVMGNHGMSIDIRHMML 1284

Query: 96   LGDLMTFRGEVLGITRFGIQKMDKSVLMLASFEKTADHLFNASVNGRDDKIEGVSECVIM 155
            L D+MT+RGEVLGI R GIQKMDKSVLM ASFE+T DHLF+A+ +G+ D IEGV+ECVIM
Sbjct: 1285 LADVMTYRGEVLGIQRTGIQKMDKSVLMQASFERTGDHLFSAAASGKVDNIEGVTECVIM 1344

Query: 156  GIPMPLGTGMLKVRQRVSVPQQLPYGPPAILS 188
            GIPM LGTG+LKV QR     +L YGP  I+S
Sbjct: 1345 GIPMKLGTGILKVLQRTDDLPKLKYGPDPIIS 1376

[Close]
BLAST of CSPI01G12600 vs. ExPASy Swiss-Prot
Match: Q5ZL98 (DNA-directed RNA polymerase III subunit RPC1 OS=Gallus gallus OX=9031 GN=POLR3A PE=2 SV=1)

HSP 1 Score: 181.8 bits (460), Expect = 7.2e-45
Identity = 92/152 (60.53%), Postives = 112/152 (73.68%), Query Frame = 0 

Query: 31   LLEPRTGLQAVMGTEGVDGCNTKSNHIIEVQQVLGIEAARKCIIEEIKYTMESHGMSIDI 90
            LL     L+AVM T GV G  T SN+  EV++ LGIEAAR  II EI+YTM +HGMSID 
Sbjct: 1225 LLVEGDNLRAVMATHGVKGTKTSSNNTYEVEKTLGIEAARTTIINEIQYTMVNHGMSIDR 1284

Query: 91   RHMMLLGDLMTFRGEVLGITRFGIQKMDKSVLMLASFEKTADHLFNASVNGRDDKIEGVS 150
            RH+MLL DLMT++GEVLGITRFG+ KM +SVLMLASFEKTADHLF+A+  G+ D + GVS
Sbjct: 1285 RHVMLLSDLMTYKGEVLGITRFGLAKMKESVLMLASFEKTADHLFDAAYFGQKDSVCGVS 1344

Query: 151  ECVIMGIPMPLGTGMLKVRQRVSVPQQLPYGP 183
            EC+IMGIPM +GTG+ K+  +       P  P
Sbjct: 1345 ECIIMGIPMNIGTGLFKLLHKADKESTPPRRP 1376

[Close]
BLAST of CSPI01G12600 vs. ExPASy Swiss-Prot
Match: A4IF62 (DNA-directed RNA polymerase III subunit RPC1 OS=Bos taurus OX=9913 GN=POLR3A PE=2 SV=1)

HSP 1 Score: 181.4 bits (459), Expect = 9.4e-45
Identity = 92/152 (60.53%), Postives = 113/152 (74.34%), Query Frame = 0 

Query: 31   LLEPRTGLQAVMGTEGVDGCNTKSNHIIEVQQVLGIEAARKCIIEEIKYTMESHGMSIDI 90
            LL     L+AVM T GV G  T SN+  EV++ LGIEAAR  II EI+YTM +HGMSID 
Sbjct: 1225 LLVEGDNLRAVMATHGVKGTRTTSNNTYEVEKTLGIEAARTTIINEIQYTMVNHGMSIDR 1284

Query: 91   RHMMLLGDLMTFRGEVLGITRFGIQKMDKSVLMLASFEKTADHLFNASVNGRDDKIEGVS 150
            RH+MLL DLMT++GEVLGITRFG+ KM +SVLMLASFEKTADHLF+A+  G+ D + GVS
Sbjct: 1285 RHVMLLSDLMTYKGEVLGITRFGLAKMKESVLMLASFEKTADHLFDAAYFGQKDSVCGVS 1344

Query: 151  ECVIMGIPMPLGTGMLKVRQRVSVPQQLPYGP 183
            EC+IMGIPM +GTG+ K+  + +     P  P
Sbjct: 1345 ECIIMGIPMNIGTGLFKLLHKANRDPSPPRRP 1376

[Close]
BLAST of CSPI01G12600 vs. ExPASy Swiss-Prot
Match: O14802 (DNA-directed RNA polymerase III subunit RPC1 OS=Homo sapiens OX=9606 GN=POLR3A PE=1 SV=2)

HSP 1 Score: 181.0 bits (458), Expect = 1.2e-44
Identity = 92/152 (60.53%), Postives = 112/152 (73.68%), Query Frame = 0 

Query: 31   LLEPRTGLQAVMGTEGVDGCNTKSNHIIEVQQVLGIEAARKCIIEEIKYTMESHGMSIDI 90
            LL     L+AVM T GV G  T SN+  EV++ LGIEAAR  II EI+YTM +HGMSID 
Sbjct: 1225 LLVEGDNLRAVMATHGVKGTRTTSNNTYEVEKTLGIEAARTTIINEIQYTMVNHGMSIDR 1284

Query: 91   RHMMLLGDLMTFRGEVLGITRFGIQKMDKSVLMLASFEKTADHLFNASVNGRDDKIEGVS 150
            RH+MLL DLMT++GEVLGITRFG+ KM +SVLMLASFEKTADHLF+A+  G+ D + GVS
Sbjct: 1285 RHVMLLSDLMTYKGEVLGITRFGLAKMKESVLMLASFEKTADHLFDAAYFGQKDSVCGVS 1344

Query: 151  ECVIMGIPMPLGTGMLKVRQRVSVPQQLPYGP 183
            EC+IMGIPM +GTG+ K+  +       P  P
Sbjct: 1345 ECIIMGIPMNIGTGLFKLLHKADRDPNPPKRP 1376

[Close]
BLAST of CSPI01G12600 vs. ExPASy Swiss-Prot
Match: Q86AQ5 (DNA-directed RNA polymerase III subunit rpc1 OS=Dictyostelium discoideum OX=44689 GN=polr3a PE=3 SV=1)

HSP 1 Score: 178.7 bits (452), Expect = 6.1e-44
Identity = 86/134 (64.18%), Postives = 107/134 (79.85%), Query Frame = 0 

Query: 38   LQAVMGTEGVDGCNTKSNHIIEVQQVLGIEAARKCIIEEIKYTMESHGMSIDIRHMMLLG 97
            L+AVM T G+ G +T SNHI+E +  LGIE AR  I+ EI   M SHGMSIDIRH+MLL 
Sbjct: 1302 LRAVMATPGIKGTHTTSNHIMECENTLGIETARNTIMSEIDMIMTSHGMSIDIRHVMLLA 1361

Query: 98   DLMTFRGEVLGITRFGIQKMDKSVLMLASFEKTADHLFNASVNGRDDKIEGVSECVIMGI 157
            DLM+F+GE+LGITRFGI KM +SVLMLASFEKT DHLF+A+V+ R D I GVSEC+IMG+
Sbjct: 1362 DLMSFKGEILGITRFGIAKMKESVLMLASFEKTTDHLFDAAVHHRQDDIVGVSECIIMGV 1421

Query: 158  PMPLGTGMLKVRQR 172
             +PLGTG+ K+ ++
Sbjct: 1422 VIPLGTGLFKLLRK 1435

[Close]
BLAST of CSPI01G12600 vs. ExPASy TrEMBL
Match: A0A0A0LXJ4 (DNA-directed RNA polymerase OS=Cucumis sativus OX=3659 GN=Csa_1G083760 PE=4 SV=1)

HSP 1 Score: 384.4 bits (986), Expect = 2.7e-103
Identity = 187/187 (100.00%), Postives = 187/187 (100.00%), Query Frame = 0 

Query: 1   MKKPKVQGCEPWGFTKGASLFFKPRHKPDLLLEPRTGLQAVMGTEGVDGCNTKSNHIIEV 60
           MKKPKVQGCEPWGFTKGASLFFKPRHKPDLLLEPRTGLQAVMGTEGVDGCNTKSNHIIEV
Sbjct: 1   MKKPKVQGCEPWGFTKGASLFFKPRHKPDLLLEPRTGLQAVMGTEGVDGCNTKSNHIIEV 60

Query: 61  QQVLGIEAARKCIIEEIKYTMESHGMSIDIRHMMLLGDLMTFRGEVLGITRFGIQKMDKS 120
           QQVLGIEAARKCIIEEIKYTMESHGMSIDIRHMMLLGDLMTFRGEVLGITRFGIQKMDKS
Sbjct: 61  QQVLGIEAARKCIIEEIKYTMESHGMSIDIRHMMLLGDLMTFRGEVLGITRFGIQKMDKS 120

Query: 121 VLMLASFEKTADHLFNASVNGRDDKIEGVSECVIMGIPMPLGTGMLKVRQRVSVPQQLPY 180
           VLMLASFEKTADHLFNASVNGRDDKIEGVSECVIMGIPMPLGTGMLKVRQRVSVPQQLPY
Sbjct: 121 VLMLASFEKTADHLFNASVNGRDDKIEGVSECVIMGIPMPLGTGMLKVRQRVSVPQQLPY 180

Query: 181 GPPAILS 188
           GPPAILS
Sbjct: 181 GPPAILS 187

[Close]
BLAST of CSPI01G12600 vs. ExPASy TrEMBL
Match: A0A5A7T0K7 (DNA-directed RNA polymerase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold359G00430 PE=4 SV=1)

HSP 1 Score: 308.1 bits (788), Expect = 2.5e-80
Identity = 154/158 (97.47%), Postives = 156/158 (98.73%), Query Frame = 0 

Query: 30  LLLEPRTGLQAVMGTEGVDGCNTKSNHIIEVQQVLGIEAARKCIIEEIKYTMESHGMSID 89
           +LL  RTGLQAVMGTEGVDGCNTKSNHIIEVQQVLGIEAARKCIIEEIKYTMESHGMSID
Sbjct: 102 ILLILRTGLQAVMGTEGVDGCNTKSNHIIEVQQVLGIEAARKCIIEEIKYTMESHGMSID 161

Query: 90  IRHMMLLGDLMTFRGEVLGITRFGIQKMDKSVLMLASFEKTADHLFNASVNGRDDKIEGV 149
           IRHMMLLGDLMTFRGEVLGITRFGIQKMDKSVLMLASFEKTADHLFNASVNGRDDKIEGV
Sbjct: 162 IRHMMLLGDLMTFRGEVLGITRFGIQKMDKSVLMLASFEKTADHLFNASVNGRDDKIEGV 221

Query: 150 SECVIMGIPMPLGTGMLKVRQRVSVPQQLPYGPPAILS 188
           SECVIMGIPMPLGTGMLKVRQRVSVP+QLPYGPPAILS
Sbjct: 222 SECVIMGIPMPLGTGMLKVRQRVSVPRQLPYGPPAILS 259

[Close]
BLAST of CSPI01G12600 vs. ExPASy TrEMBL
Match: A0A1S3CNX9 (DNA-directed RNA polymerase subunit OS=Cucumis melo OX=3656 GN=LOC103502942 PE=3 SV=1)

HSP 1 Score: 305.8 bits (782), Expect = 1.2e-79
Identity = 153/157 (97.45%), Postives = 154/157 (98.09%), Query Frame = 0 

Query: 31   LLEPRTGLQAVMGTEGVDGCNTKSNHIIEVQQVLGIEAARKCIIEEIKYTMESHGMSIDI 90
            LL   TGLQAVMGTEGVDGCNTKSNHIIEVQQVLGIEAARKCIIEEIKYTMESHGMSIDI
Sbjct: 1244 LLVEGTGLQAVMGTEGVDGCNTKSNHIIEVQQVLGIEAARKCIIEEIKYTMESHGMSIDI 1303

Query: 91   RHMMLLGDLMTFRGEVLGITRFGIQKMDKSVLMLASFEKTADHLFNASVNGRDDKIEGVS 150
            RHMMLLGDLMTFRGEVLGITRFGIQKMDKSVLMLASFEKTADHLFNASVNGRDDKIEGVS
Sbjct: 1304 RHMMLLGDLMTFRGEVLGITRFGIQKMDKSVLMLASFEKTADHLFNASVNGRDDKIEGVS 1363

Query: 151  ECVIMGIPMPLGTGMLKVRQRVSVPQQLPYGPPAILS 188
            ECVIMGIPMPLGTGMLKVRQRVSVP+QLPYGPPAILS
Sbjct: 1364 ECVIMGIPMPLGTGMLKVRQRVSVPRQLPYGPPAILS 1400

[Close]
BLAST of CSPI01G12600 vs. ExPASy TrEMBL
Match: A0A6J1I0L0 (DNA-directed RNA polymerase subunit OS=Cucurbita maxima OX=3661 GN=LOC111469417 PE=3 SV=1)

HSP 1 Score: 303.9 bits (777), Expect = 4.6e-79
Identity = 152/157 (96.82%), Postives = 152/157 (96.82%), Query Frame = 0 

Query: 31   LLEPRTGLQAVMGTEGVDGCNTKSNHIIEVQQVLGIEAARKCIIEEIKYTMESHGMSIDI 90
            LL   TGLQAVMGTEGVDGCNTKSNHIIEVQQVLGIEAARKCIIEEIKYTMESHGMSIDI
Sbjct: 1244 LLVEGTGLQAVMGTEGVDGCNTKSNHIIEVQQVLGIEAARKCIIEEIKYTMESHGMSIDI 1303

Query: 91   RHMMLLGDLMTFRGEVLGITRFGIQKMDKSVLMLASFEKTADHLFNASVNGRDDKIEGVS 150
            RHMMLLGDLMTFRGEVLGITRFGIQKMDKSVLMLASFEKTADHLFNASVNGRDDKIEGVS
Sbjct: 1304 RHMMLLGDLMTFRGEVLGITRFGIQKMDKSVLMLASFEKTADHLFNASVNGRDDKIEGVS 1363

Query: 151  ECVIMGIPMPLGTGMLKVRQRVSVPQQLPYGPPAILS 188
            ECVIMGIPMPLGTGMLKVRQR SVP QLPYGPPAILS
Sbjct: 1364 ECVIMGIPMPLGTGMLKVRQRASVPHQLPYGPPAILS 1400

[Close]
BLAST of CSPI01G12600 vs. ExPASy TrEMBL
Match: A0A6J1HLT9 (DNA-directed RNA polymerase subunit OS=Cucurbita moschata OX=3662 GN=LOC111464789 PE=3 SV=1)

HSP 1 Score: 303.9 bits (777), Expect = 4.6e-79
Identity = 152/157 (96.82%), Postives = 152/157 (96.82%), Query Frame = 0 

Query: 31   LLEPRTGLQAVMGTEGVDGCNTKSNHIIEVQQVLGIEAARKCIIEEIKYTMESHGMSIDI 90
            LL   TGLQAVMGTEGVDGCNTKSNHIIEVQQVLGIEAARKCIIEEIKYTMESHGMSIDI
Sbjct: 1244 LLVEGTGLQAVMGTEGVDGCNTKSNHIIEVQQVLGIEAARKCIIEEIKYTMESHGMSIDI 1303

Query: 91   RHMMLLGDLMTFRGEVLGITRFGIQKMDKSVLMLASFEKTADHLFNASVNGRDDKIEGVS 150
            RHMMLLGDLMTFRGEVLGITRFGIQKMDKSVLMLASFEKTADHLFNASVNGRDDKIEGVS
Sbjct: 1304 RHMMLLGDLMTFRGEVLGITRFGIQKMDKSVLMLASFEKTADHLFNASVNGRDDKIEGVS 1363

Query: 151  ECVIMGIPMPLGTGMLKVRQRVSVPQQLPYGPPAILS 188
            ECVIMGIPMPLGTGMLKVRQR SVP QLPYGPPAILS
Sbjct: 1364 ECVIMGIPMPLGTGMLKVRQRASVPHQLPYGPPAILS 1400

[Close]
BLAST of CSPI01G12600 vs. NCBI nr
Match: KAA0036972.1 (DNA-directed RNA polymerase [Cucumis melo var. makuwa] >TYK21234.1 DNA-directed RNA polymerase [Cucumis melo var. makuwa])

HSP 1 Score: 308.1 bits (788), Expect = 5.1e-80
Identity = 154/158 (97.47%), Postives = 156/158 (98.73%), Query Frame = 0 

Query: 30  LLLEPRTGLQAVMGTEGVDGCNTKSNHIIEVQQVLGIEAARKCIIEEIKYTMESHGMSID 89
           +LL  RTGLQAVMGTEGVDGCNTKSNHIIEVQQVLGIEAARKCIIEEIKYTMESHGMSID
Sbjct: 102 ILLILRTGLQAVMGTEGVDGCNTKSNHIIEVQQVLGIEAARKCIIEEIKYTMESHGMSID 161

Query: 90  IRHMMLLGDLMTFRGEVLGITRFGIQKMDKSVLMLASFEKTADHLFNASVNGRDDKIEGV 149
           IRHMMLLGDLMTFRGEVLGITRFGIQKMDKSVLMLASFEKTADHLFNASVNGRDDKIEGV
Sbjct: 162 IRHMMLLGDLMTFRGEVLGITRFGIQKMDKSVLMLASFEKTADHLFNASVNGRDDKIEGV 221

Query: 150 SECVIMGIPMPLGTGMLKVRQRVSVPQQLPYGPPAILS 188
           SECVIMGIPMPLGTGMLKVRQRVSVP+QLPYGPPAILS
Sbjct: 222 SECVIMGIPMPLGTGMLKVRQRVSVPRQLPYGPPAILS 259

[Close]
BLAST of CSPI01G12600 vs. NCBI nr
Match: XP_004148776.1 (DNA-directed RNA polymerase III subunit 1 isoform X1 [Cucumis sativus] >KAE8652732.1 hypothetical protein Csa_013764 [Cucumis sativus])

HSP 1 Score: 307.4 bits (786), Expect = 8.6e-80
Identity = 154/157 (98.09%), Postives = 154/157 (98.09%), Query Frame = 0 

Query: 31   LLEPRTGLQAVMGTEGVDGCNTKSNHIIEVQQVLGIEAARKCIIEEIKYTMESHGMSIDI 90
            LL   TGLQAVMGTEGVDGCNTKSNHIIEVQQVLGIEAARKCIIEEIKYTMESHGMSIDI
Sbjct: 1244 LLVEGTGLQAVMGTEGVDGCNTKSNHIIEVQQVLGIEAARKCIIEEIKYTMESHGMSIDI 1303

Query: 91   RHMMLLGDLMTFRGEVLGITRFGIQKMDKSVLMLASFEKTADHLFNASVNGRDDKIEGVS 150
            RHMMLLGDLMTFRGEVLGITRFGIQKMDKSVLMLASFEKTADHLFNASVNGRDDKIEGVS
Sbjct: 1304 RHMMLLGDLMTFRGEVLGITRFGIQKMDKSVLMLASFEKTADHLFNASVNGRDDKIEGVS 1363

Query: 151  ECVIMGIPMPLGTGMLKVRQRVSVPQQLPYGPPAILS 188
            ECVIMGIPMPLGTGMLKVRQRVSVPQQLPYGPPAILS
Sbjct: 1364 ECVIMGIPMPLGTGMLKVRQRVSVPQQLPYGPPAILS 1400

[Close]
BLAST of CSPI01G12600 vs. NCBI nr
Match: XP_031736287.1 (DNA-directed RNA polymerase III subunit 1 isoform X2 [Cucumis sativus])

HSP 1 Score: 307.4 bits (786), Expect = 8.6e-80
Identity = 154/157 (98.09%), Postives = 154/157 (98.09%), Query Frame = 0 

Query: 31   LLEPRTGLQAVMGTEGVDGCNTKSNHIIEVQQVLGIEAARKCIIEEIKYTMESHGMSIDI 90
            LL   TGLQAVMGTEGVDGCNTKSNHIIEVQQVLGIEAARKCIIEEIKYTMESHGMSIDI
Sbjct: 1059 LLVEGTGLQAVMGTEGVDGCNTKSNHIIEVQQVLGIEAARKCIIEEIKYTMESHGMSIDI 1118

Query: 91   RHMMLLGDLMTFRGEVLGITRFGIQKMDKSVLMLASFEKTADHLFNASVNGRDDKIEGVS 150
            RHMMLLGDLMTFRGEVLGITRFGIQKMDKSVLMLASFEKTADHLFNASVNGRDDKIEGVS
Sbjct: 1119 RHMMLLGDLMTFRGEVLGITRFGIQKMDKSVLMLASFEKTADHLFNASVNGRDDKIEGVS 1178

Query: 151  ECVIMGIPMPLGTGMLKVRQRVSVPQQLPYGPPAILS 188
            ECVIMGIPMPLGTGMLKVRQRVSVPQQLPYGPPAILS
Sbjct: 1179 ECVIMGIPMPLGTGMLKVRQRVSVPQQLPYGPPAILS 1215

[Close]
BLAST of CSPI01G12600 vs. NCBI nr
Match: XP_008465290.2 (PREDICTED: LOW QUALITY PROTEIN: DNA-directed RNA polymerase III subunit 1 [Cucumis melo])

HSP 1 Score: 305.8 bits (782), Expect = 2.5e-79
Identity = 153/157 (97.45%), Postives = 154/157 (98.09%), Query Frame = 0 

Query: 31   LLEPRTGLQAVMGTEGVDGCNTKSNHIIEVQQVLGIEAARKCIIEEIKYTMESHGMSIDI 90
            LL   TGLQAVMGTEGVDGCNTKSNHIIEVQQVLGIEAARKCIIEEIKYTMESHGMSIDI
Sbjct: 1244 LLVEGTGLQAVMGTEGVDGCNTKSNHIIEVQQVLGIEAARKCIIEEIKYTMESHGMSIDI 1303

Query: 91   RHMMLLGDLMTFRGEVLGITRFGIQKMDKSVLMLASFEKTADHLFNASVNGRDDKIEGVS 150
            RHMMLLGDLMTFRGEVLGITRFGIQKMDKSVLMLASFEKTADHLFNASVNGRDDKIEGVS
Sbjct: 1304 RHMMLLGDLMTFRGEVLGITRFGIQKMDKSVLMLASFEKTADHLFNASVNGRDDKIEGVS 1363

Query: 151  ECVIMGIPMPLGTGMLKVRQRVSVPQQLPYGPPAILS 188
            ECVIMGIPMPLGTGMLKVRQRVSVP+QLPYGPPAILS
Sbjct: 1364 ECVIMGIPMPLGTGMLKVRQRVSVPRQLPYGPPAILS 1400

[Close]
BLAST of CSPI01G12600 vs. NCBI nr
Match: KAG6583479.1 (DNA-directed RNA polymerase III subunit 1, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 303.9 bits (777), Expect = 9.6e-79
Identity = 152/157 (96.82%), Postives = 152/157 (96.82%), Query Frame = 0 

Query: 31   LLEPRTGLQAVMGTEGVDGCNTKSNHIIEVQQVLGIEAARKCIIEEIKYTMESHGMSIDI 90
            LL   TGLQAVMGTEGVDGCNTKSNHIIEVQQVLGIEAARKCIIEEIKYTMESHGMSIDI
Sbjct: 1244 LLVEGTGLQAVMGTEGVDGCNTKSNHIIEVQQVLGIEAARKCIIEEIKYTMESHGMSIDI 1303

Query: 91   RHMMLLGDLMTFRGEVLGITRFGIQKMDKSVLMLASFEKTADHLFNASVNGRDDKIEGVS 150
            RHMMLLGDLMTFRGEVLGITRFGIQKMDKSVLMLASFEKTADHLFNASVNGRDDKIEGVS
Sbjct: 1304 RHMMLLGDLMTFRGEVLGITRFGIQKMDKSVLMLASFEKTADHLFNASVNGRDDKIEGVS 1363

Query: 151  ECVIMGIPMPLGTGMLKVRQRVSVPQQLPYGPPAILS 188
            ECVIMGIPMPLGTGMLKVRQR SVP QLPYGPPAILS
Sbjct: 1364 ECVIMGIPMPLGTGMLKVRQRASVPHQLPYGPPAILS 1400

[Close]
BLAST of CSPI01G12600 vs. TAIR 10
Match: AT5G60040.1 (nuclear RNA polymerase C1 )

HSP 1 Score: 200.3 bits (508), Expect = 1.4e-51
Identity = 101/152 (66.45%), Postives = 120/152 (78.95%), Query Frame = 0 

Query: 36   TGLQAVMGTEGVDGCNTKSNHIIEVQQVLGIEAARKCIIEEIKYTMESHGMSIDIRHMML 95
            T L AVMGT G++G  T SN+++EV + LGIEAAR  II+EI   M +HGMSIDIRHMML
Sbjct: 1225 TNLLAVMGTPGINGRTTTSNNVVEVSKTLGIEAARTTIIDEIGTVMGNHGMSIDIRHMML 1284

Query: 96   LGDLMTFRGEVLGITRFGIQKMDKSVLMLASFEKTADHLFNASVNGRDDKIEGVSECVIM 155
            L D+MT+RGEVLGI R GIQKMDKSVLM ASFE+T DHLF+A+ +G+ D IEGV+ECVIM
Sbjct: 1285 LADVMTYRGEVLGIQRTGIQKMDKSVLMQASFERTGDHLFSAAASGKVDNIEGVTECVIM 1344

Query: 156  GIPMPLGTGMLKVRQRVSVPQQLPYGPPAILS 188
            GIPM LGTG+LKV QR     +L YGP  I+S
Sbjct: 1345 GIPMKLGTGILKVLQRTDDLPKLKYGPDPIIS 1376

[Close]
BLAST of CSPI01G12600 vs. TAIR 10
Match: AT5G60040.2 (nuclear RNA polymerase C1 )

HSP 1 Score: 200.3 bits (508), Expect = 1.4e-51
Identity = 101/152 (66.45%), Postives = 120/152 (78.95%), Query Frame = 0 

Query: 36   TGLQAVMGTEGVDGCNTKSNHIIEVQQVLGIEAARKCIIEEIKYTMESHGMSIDIRHMML 95
            T L AVMGT G++G  T SN+++EV + LGIEAAR  II+EI   M +HGMSIDIRHMML
Sbjct: 1240 TNLLAVMGTPGINGRTTTSNNVVEVSKTLGIEAARTTIIDEIGTVMGNHGMSIDIRHMML 1299

Query: 96   LGDLMTFRGEVLGITRFGIQKMDKSVLMLASFEKTADHLFNASVNGRDDKIEGVSECVIM 155
            L D+MT+RGEVLGI R GIQKMDKSVLM ASFE+T DHLF+A+ +G+ D IEGV+ECVIM
Sbjct: 1300 LADVMTYRGEVLGIQRTGIQKMDKSVLMQASFERTGDHLFSAAASGKVDNIEGVTECVIM 1359

Query: 156  GIPMPLGTGMLKVRQRVSVPQQLPYGPPAILS 188
            GIPM LGTG+LKV QR     +L YGP  I+S
Sbjct: 1360 GIPMKLGTGILKVLQRTDDLPKLKYGPDPIIS 1391

[Close]
BLAST of CSPI01G12600 vs. TAIR 10
Match: AT4G35800.1 (RNA polymerase II large subunit )

HSP 1 Score: 109.8 bits (273), Expect = 2.5e-24
Identity = 54/127 (42.52%), Postives = 81/127 (63.78%), Query Frame = 0 

Query: 38   LQAVMGTEGVDGCNTKSNHIIEVQQVLGIEAARKCIIEEIKYTMESHGMSIDIRHMMLLG 97
            L AVM  E VD   T SNH+IE+ +VLGIEA R+ +++E++  +   G  ++ RH+ +L 
Sbjct: 1334 LLAVMCHEDVDPKRTTSNHLIEIIEVLGIEAVRRALLDELRVVISFDGSYVNYRHLAILC 1393

Query: 98   DLMTFRGEVLGITRFGIQKMDKSVLMLASFEKTADHLFNASVNGRDDKIEGVSECVIMGI 157
            D MT+RG ++ ITR GI + D   LM  SFE+T D L +A+     D + GV+E +++G 
Sbjct: 1394 DTMTYRGHLMAITRHGINRNDTGPLMRCSFEETVDILLDAAAYAETDCLRGVTENIMLGQ 1453

Query: 158  PMPLGTG 165
              P+GTG
Sbjct: 1454 LAPIGTG 1460

[Close]
BLAST of CSPI01G12600 vs. TAIR 10
Match: AT3G57660.1 (nuclear RNA polymerase A1 )

HSP 1 Score: 80.1 bits (196), Expect = 2.1e-15
Identity = 42/121 (34.71%), Postives = 65/121 (53.72%), Query Frame = 0 

Query: 54   SNHIIEVQQVLGIEAARKCIIEEIKYTMESHGMSIDIRHMMLLGDLMTFRGEVLGITRFG 113
            SN I ++  + G+EAAR+ II EI +  +S+G+S+ IRH+ L+ D MTF G    ++R G
Sbjct: 1550 SNSIHDMLNIFGVEAARETIIREINHVFKSYGISVSIRHLNLIADYMTFSGGYRPMSRMG 1609

Query: 114  IQKMDKSVLMLASFEKTADHLFNASVNGRDDKIEGVSECVIMGIPMPLGTGMLKVRQRVS 173
                  S     +FE     +  A+  G  D +E  S  + +G+P   GTG   + QRV 
Sbjct: 1610 GIAESTSPFCRMTFETATKFIVQAATYGEKDTLETPSARICLGLPALSGTGCFDLMQRVE 1669

Query: 174  V 175
            +
Sbjct: 1670 L 1670

[Close]
BLAST of CSPI01G12600 vs. TAIR 10
Match: ATCG00170.1 (DNA-directed RNA polymerase family protein )

HSP 1 Score: 53.5 bits (127), Expect = 2.1e-07
Identity = 48/162 (29.63%), Postives = 77/162 (47.53%), Query Frame = 0 

Query: 11   PWGFTKGASLFFKPRHKPDLLLEPRTGL----QAVMGTEGVDGCNTKSNHI-IEVQQVLG 70
            PWGF  GA L          +++ R  L    Q V  ++GV      + HI I V+Q+  
Sbjct: 1178 PWGFLIGAEL---------TIVQSRISLVNKIQKVYRSQGV---QIHNRHIEIIVRQITS 1237

Query: 71   IEAARKCIIEEIKYTMESHGMSIDIRHMMLLGDLMTFRGEVLGITRFGIQKMDKSVLMLA 130
                 +  +  +    E  G+    R    L + + +R  +LGITR  +    +S +  A
Sbjct: 1238 KVLVSEEGMSNVFLPGELIGLLRAERTGRALEEAICYRAVLLGITRASLN--TQSFISEA 1297

Query: 131  SFEKTADHLFNASVNGRDDKIEGVSECVIMGIPMPLGTGMLK 168
            SF++TA  L  A++ GR D ++G+ E V++G  +P GTG  K
Sbjct: 1298 SFQETARVLAKAALRGRIDWLKGLKENVVLGGVIPAGTGFNK 1325

The following BLAST results are available for this feature:
BLAST of CSPI01G12600 vs. ExPASy Swiss-Prot
Match NameE-valueIdentityDescription
F4JXF92.0e-5066.45DNA-directed RNA polymerase III subunit 1 OS=Arabidopsis thaliana OX=3702 GN=NRP... [more]
Q5ZL987.2e-4560.53DNA-directed RNA polymerase III subunit RPC1 OS=Gallus gallus OX=9031 GN=POLR3A ... [more]
A4IF629.4e-4560.53DNA-directed RNA polymerase III subunit RPC1 OS=Bos taurus OX=9913 GN=POLR3A PE=... [more]
O148021.2e-4460.53DNA-directed RNA polymerase III subunit RPC1 OS=Homo sapiens OX=9606 GN=POLR3A P... [more]
Q86AQ56.1e-4464.18DNA-directed RNA polymerase III subunit rpc1 OS=Dictyostelium discoideum OX=4468... [more]
BLAST of CSPI01G12600 vs. ExPASy TrEMBL
Match NameE-valueIdentityDescription
A0A0A0LXJ42.7e-103100.00DNA-directed RNA polymerase OS=Cucumis sativus OX=3659 GN=Csa_1G083760 PE=4 SV=1[more]
A0A5A7T0K72.5e-8097.47DNA-directed RNA polymerase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaf... [more]
A0A1S3CNX91.2e-7997.45DNA-directed RNA polymerase subunit OS=Cucumis melo OX=3656 GN=LOC103502942 PE=3... [more]
A0A6J1I0L04.6e-7996.82DNA-directed RNA polymerase subunit OS=Cucurbita maxima OX=3661 GN=LOC111469417 ... [more]
A0A6J1HLT94.6e-7996.82DNA-directed RNA polymerase subunit OS=Cucurbita moschata OX=3662 GN=LOC11146478... [more]
BLAST of CSPI01G12600 vs. NCBI nr
Match NameE-valueIdentityDescription
KAA0036972.15.1e-8097.47DNA-directed RNA polymerase [Cucumis melo var. makuwa] >TYK21234.1 DNA-directed ... [more]
XP_004148776.18.6e-8098.09DNA-directed RNA polymerase III subunit 1 isoform X1 [Cucumis sativus] >KAE86527... [more]
XP_031736287.18.6e-8098.09DNA-directed RNA polymerase III subunit 1 isoform X2 [Cucumis sativus][more]
XP_008465290.22.5e-7997.45PREDICTED: LOW QUALITY PROTEIN: DNA-directed RNA polymerase III subunit 1 [Cucum... [more]
KAG6583479.19.6e-7996.82DNA-directed RNA polymerase III subunit 1, partial [Cucurbita argyrosperma subsp... [more]
BLAST of CSPI01G12600 vs. TAIR 10
Match NameE-valueIdentityDescription
AT5G60040.11.4e-5166.45nuclear RNA polymerase C1 [more]
AT5G60040.21.4e-5166.45nuclear RNA polymerase C1 [more]
AT4G35800.12.5e-2442.52RNA polymerase II large subunit [more]
AT3G57660.12.1e-1534.71nuclear RNA polymerase A1 [more]
ATCG00170.12.1e-0729.63DNA-directed RNA polymerase family protein [more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (PI 183967) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableGENE3D1.10.150.390coord: 119..178
e-value: 2.8E-21
score: 76.8
NoneNo IPR availablePANTHERPTHR19376:SF32DNA-DIRECTED RNA POLYMERASE III SUBUNIT RPC1coord: 33..172
NoneNo IPR availablePANTHERPTHR19376DNA-DIRECTED RNA POLYMERASEcoord: 33..172
NoneNo IPR availableSUPERFAMILY64484beta and beta-prime subunits of DNA dependent RNA-polymerasecoord: 31..171
IPR007081RNA polymerase Rpb1, domain 5PFAMPF04998RNA_pol_Rpb1_5coord: 36..121
e-value: 1.1E-18
score: 67.6

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CSPI01G12600.1CSPI01G12600.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006351 transcription, DNA-templated
cellular_component GO:0005666 RNA polymerase III complex
molecular_function GO:0003677 DNA binding
molecular_function GO:0003899 DNA-directed 5'-3' RNA polymerase activity
molecular_function GO:0046872 metal ion binding