CSPI01G03700 (gene) Cucumber (PI 183967) v1

Overview


Name	CSPI01G03700
Type	gene
Organism	Cucumis sativus var. hardwickii cv. PI 183967 (Cucumber (PI 183967) v1)
Description	Protein ASPARTIC PROTEASE IN GUARD CELL 1-like
Location	Chr1: 2317239 .. 2318976 (+)
RNA-Seq Expression	CSPI01G03700
Synteny	CSPI01G03700

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDSpolypeptidethree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

GTCTTCACTTCCATCTTCATCCAATAATTCATTATCTTTTCATTCCCAACATGAACACTTCACTTTCCTCTGTTTTTCTCTTCCTAACAATCTTCACTTCCCTTCAATTCCCTTCAATCCTCTCTCGCAAGTTAACACCATCTTCCTATTCCACTTCCATCTTCGATGTCTCTGCCTCCACAAACCAAGCCCTAGATGCCCTCTCCATTAAACCCAAACCTCTTCAAAATCACTCTCACCTTCCAAATTCCCCTTTCTCTCTGCCATTGTACCCTAGATTGGCCCTTCATAACCCTTCTTACAAGGACTACAATACCCTTGTTAGGGCCCGACTCACTCGTGATGCCGCTCGAGTTCAATTCCTTAACCGAAATCTTGAGCGCTCTTTAAACGGGGGTACTCATTTTGGTGAAAGTATTAATGAATCTCTAACTGGAGATTCAATTACTGCTCCGGTTGTTTCGGGGCAAAGTAAAGGGAGTGGTGCTGAGTATTTAGCTCAAATTGGGGTTGGTCAGCCTGTGAAGTTGTTTTATTTGGTGCCTGATACTGGTAGCGATGTCACGTGGCTTCAATGTCAACCTTGTGCTAGTGAGAATACTTGTTATAAACAATTTGACCCTATTTTCGATCCGAAATCATCGTCTTCTTACAGTCCTCTGTCTTGCAATTCTCAACAATGTAAGTTGCTAGATAAAGCCAATTGCAATTCCGACACATGCATATACCAAGTCCACTACGGTGATGGATCATTCACAACTGGTGAACTCGCCACCGAAACACTATCGTTTGGAAATTCAAATTCTATTCCTAATCTCCCAATTGGTTGTGGGCATGACAATGAAGGCTTGTTTGCTGGTGGAGCTGGTTTAATAGGCCTCGGTGGTGGGGCCATTTCCCTTTCTTCCCAATTAAAAGCGTCATCATTTTCATATTGCCTCGTCAACTTAGATTCAGACTCATCCTCCACTCTTGAGTTTAACTCAAACATGCCCAGTGACTCGTTGACCTCTCCGCTCGTGAAAAACGATCGATTTCACTCGTATAGGTACGTCAAAGTCGTTGGAATAAGTGTTGGGGGAAAAACTCTACCAATTTCACCGACAAGATTTGAAATTGATGAATCGGGGTTGGGAGGAATAATCGTTGATTCTGGTACAATTATATCTCGACTACCGAGTGATGTCTATGAATCATTAAGAGAGGCATTTGTGAAGCTGACGAGTAGCCTCTCACCGGCACCAGGGATATCAGTGTTCGATACATGTTATAACTTTTCAGGTCAATCGAATGTGGAGGTCCCAACAATAGCATTTGTGTTGTCGGAAGGAACCTCGCTACGACTACCTGCAAGAAATTACTTAATTATGTTGGACACAGCAGGAACTTATTGTTTGGCGTTTATCAAAACGAAATCTTCACTTTCTATAATTGGTAGCTTCCAACAACAAGGAATACGTGTTAGTTATGACTTGACAAACTCCCTCGTTGGATTCTCAACTAATAAATGTTAGTACCATAAACTTATAATAACTCATGTAACACCACGTGGGATTAGAATTTGGCTTTAATCACATTGTTCTAAGTCAATAAAATATTGTTGTTTATCATACACATGGGCTTTGCGTACGAAAGTCTTAATTTAGTAGTGTGTTGAACTAAGCAAAAATAAAACAAAAACAAAAAAAGTTTCTTTCTTCGTATTGTTATCGTTCCTAAGAAACAAATTATAGAGAAG

mRNA sequence

GTCTTCACTTCCATCTTCATCCAATAATTCATTATCTTTTCATTCCCAACATGAACACTTCACTTTCCTCTGTTTTTCTCTTCCTAACAATCTTCACTTCCCTTCAATTCCCTTCAATCCTCTCTCGCAAGTTAACACCATCTTCCTATTCCACTTCCATCTTCGATGTCTCTGCCTCCACAAACCAAGCCCTAGATGCCCTCTCCATTAAACCCAAACCTCTTCAAAATCACTCTCACCTTCCAAATTCCCCTTTCTCTCTGCCATTGTACCCTAGATTGGCCCTTCATAACCCTTCTTACAAGGACTACAATACCCTTGTTAGGGCCCGACTCACTCGTGATGCCGCTCGAGTTCAATTCCTTAACCGAAATCTTGAGCGCTCTTTAAACGGGGGTACTCATTTTGGTGAAAGTATTAATGAATCTCTAACTGGAGATTCAATTACTGCTCCGGTTGTTTCGGGGCAAAGTAAAGGGAGTGGTGCTGAGTATTTAGCTCAAATTGGGGTTGGTCAGCCTGTGAAGTTGTTTTATTTGGTGCCTGATACTGGTAGCGATGTCACGTGGCTTCAATGTCAACCTTGTGCTAGTGAGAATACTTGTTATAAACAATTTGACCCTATTTTCGATCCGAAATCATCGTCTTCTTACAGTCCTCTGTCTTGCAATTCTCAACAATGTAAGTTGCTAGATAAAGCCAATTGCAATTCCGACACATGCATATACCAAGTCCACTACGGTGATGGATCATTCACAACTGGTGAACTCGCCACCGAAACACTATCGTTTGGAAATTCAAATTCTATTCCTAATCTCCCAATTGGTTGTGGGCATGACAATGAAGGCTTGTTTGCTGGTGGAGCTGGTTTAATAGGCCTCGGTGGTGGGGCCATTTCCCTTTCTTCCCAATTAAAAGCGTCATCATTTTCATATTGCCTCGTCAACTTAGATTCAGACTCATCCTCCACTCTTGAGTTTAACTCAAACATGCCCAGTGACTCGTTGACCTCTCCGCTCGTGAAAAACGATCGATTTCACTCGTATAGGTACGTCAAAGTCGTTGGAATAAGTGTTGGGGGAAAAACTCTACCAATTTCACCGACAAGATTTGAAATTGATGAATCGGGGTTGGGAGGAATAATCGTTGATTCTGGTACAATTATATCTCGACTACCGAGTGATGTCTATGAATCATTAAGAGAGGCATTTGTGAAGCTGACGAGTAGCCTCTCACCGGCACCAGGGATATCAGTGTTCGATACATGTTATAACTTTTCAGGTCAATCGAATGTGGAGGTCCCAACAATAGCATTTGTGTTGTCGGAAGGAACCTCGCTACGACTACCTGCAAGAAATTACTTAATTATGTTGGACACAGCAGGAACTTATTGTTTGGCGTTTATCAAAACGAAATCTTCACTTTCTATAATTGGTAGCTTCCAACAACAAGGAATACGTGTTAGTTATGACTTGACAAACTCCCTCGTTGGATTCTCAACTAATAAATGTTAGTACCATAAACTTATAATAACTCATGTAACACCACGTGGGATTAGAATTTGGCTTTAATCACATTGTTCTAAGTCAATAAAATATTGTTGTTTATCATACACATGGGCTTTGCGTACGAAAGTCTTAATTTAGTAGTGTGTTGAACTAAGCAAAAATAAAACAAAAACAAAAAAAGTTTCTTTCTTCGTATTGTTATCGTTCCTAAGAAACAAATTATAGAGAAG

Coding sequence (CDS)

ATGAACACTTCACTTTCCTCTGTTTTTCTCTTCCTAACAATCTTCACTTCCCTTCAATTCCCTTCAATCCTCTCTCGCAAGTTAACACCATCTTCCTATTCCACTTCCATCTTCGATGTCTCTGCCTCCACAAACCAAGCCCTAGATGCCCTCTCCATTAAACCCAAACCTCTTCAAAATCACTCTCACCTTCCAAATTCCCCTTTCTCTCTGCCATTGTACCCTAGATTGGCCCTTCATAACCCTTCTTACAAGGACTACAATACCCTTGTTAGGGCCCGACTCACTCGTGATGCCGCTCGAGTTCAATTCCTTAACCGAAATCTTGAGCGCTCTTTAAACGGGGGTACTCATTTTGGTGAAAGTATTAATGAATCTCTAACTGGAGATTCAATTACTGCTCCGGTTGTTTCGGGGCAAAGTAAAGGGAGTGGTGCTGAGTATTTAGCTCAAATTGGGGTTGGTCAGCCTGTGAAGTTGTTTTATTTGGTGCCTGATACTGGTAGCGATGTCACGTGGCTTCAATGTCAACCTTGTGCTAGTGAGAATACTTGTTATAAACAATTTGACCCTATTTTCGATCCGAAATCATCGTCTTCTTACAGTCCTCTGTCTTGCAATTCTCAACAATGTAAGTTGCTAGATAAAGCCAATTGCAATTCCGACACATGCATATACCAAGTCCACTACGGTGATGGATCATTCACAACTGGTGAACTCGCCACCGAAACACTATCGTTTGGAAATTCAAATTCTATTCCTAATCTCCCAATTGGTTGTGGGCATGACAATGAAGGCTTGTTTGCTGGTGGAGCTGGTTTAATAGGCCTCGGTGGTGGGGCCATTTCCCTTTCTTCCCAATTAAAAGCGTCATCATTTTCATATTGCCTCGTCAACTTAGATTCAGACTCATCCTCCACTCTTGAGTTTAACTCAAACATGCCCAGTGACTCGTTGACCTCTCCGCTCGTGAAAAACGATCGATTTCACTCGTATAGGTACGTCAAAGTCGTTGGAATAAGTGTTGGGGGAAAAACTCTACCAATTTCACCGACAAGATTTGAAATTGATGAATCGGGGTTGGGAGGAATAATCGTTGATTCTGGTACAATTATATCTCGACTACCGAGTGATGTCTATGAATCATTAAGAGAGGCATTTGTGAAGCTGACGAGTAGCCTCTCACCGGCACCAGGGATATCAGTGTTCGATACATGTTATAACTTTTCAGGTCAATCGAATGTGGAGGTCCCAACAATAGCATTTGTGTTGTCGGAAGGAACCTCGCTACGACTACCTGCAAGAAATTACTTAATTATGTTGGACACAGCAGGAACTTATTGTTTGGCGTTTATCAAAACGAAATCTTCACTTTCTATAATTGGTAGCTTCCAACAACAAGGAATACGTGTTAGTTATGACTTGACAAACTCCCTCGTTGGATTCTCAACTAATAAATGTTAG

Protein sequence

MNTSLSSVFLFLTIFTSLQFPSILSRKLTPSSYSTSIFDVSASTNQALDALSIKPKPLQNHSHLPNSPFSLPLYPRLALHNPSYKDYNTLVRARLTRDAARVQFLNRNLERSLNGGTHFGESINESLTGDSITAPVVSGQSKGSGAEYLAQIGVGQPVKLFYLVPDTGSDVTWLQCQPCASENTCYKQFDPIFDPKSSSSYSPLSCNSQQCKLLDKANCNSDTCIYQVHYGDGSFTTGELATETLSFGNSNSIPNLPIGCGHDNEGLFAGGAGLIGLGGGAISLSSQLKASSFSYCLVNLDSDSSSTLEFNSNMPSDSLTSPLVKNDRFHSYRYVKVVGISVGGKTLPISPTRFEIDESGLGGIIVDSGTIISRLPSDVYESLREAFVKLTSSLSPAPGISVFDTCYNFSGQSNVEVPTIAFVLSEGTSLRLPARNYLIMLDTAGTYCLAFIKTKSSLSIIGSFQQQGIRVSYDLTNSLVGFSTNKC*

Homology

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BLAST of CSPI01G03700 vs. ExPASy Swiss-Prot
Match: Q9LS40 (Protein ASPARTIC PROTEASE IN GUARD CELL 1 OS=Arabidopsis thaliana OX=3702 GN=ASPG1 PE=1 SV=1)

HSP 1 Score: 423.7 bits (1088), Expect = 2.8e-117
Identity = 234/498 (46.99%), Postives = 328/498 (65.86%), Query Frame = 0


Query: 7   SVFLFLTIFTSLQFPSILSRKLTPSSYSTSIFDVSASTNQALDALSI----------KPK 66
           S+   +T+   L      SR L+ +   T++ DV +S  Q    LS+          KP+
Sbjct: 8   SLLAVVTLSLFLTTTDASSRSLS-TPPKTNVLDVVSSLQQTQTILSLDPTRSSLTTTKPE 67

Query: 67  PLQNHSHL-PNSPFSLPLYPRLALHNPSYKDYNTLVRARLTRDAARVQFLNRNLERSLNG 126
            L +      +SP SL L+ R       +KDY +L  +RL RD++RV  +   +  ++ G
Sbjct: 68  SLSDPVFFNSSSPLSLELHSRDTFVASQHKDYKSLTLSRLERDSSRVAGIVAKIRFAVEG 127

Query: 127 --GTHFGESINES--LTGDSITAPVVSGQSKGSGAEYLAQIGVGQPVKLFYLVPDTGSDV 186
              +      NE      + +T PVVSG S+GSG EY ++IGVG P K  YLV DTGSDV
Sbjct: 128 VDRSDLKPVYNEDTRYQTEDLTTPVVSGASQGSG-EYFSRIGVGTPAKEMYLVLDTGSDV 187

Query: 187 TWLQCQPCASENTCYKQFDPIFDPKSSSSYSPLSCNSQQCKLLDKANCNSDTCIYQVHYG 246
            W+QC+PCA    CY+Q DP+F+P SSS+Y  L+C++ QC LL+ + C S+ C+YQV YG
Sbjct: 188 NWIQCEPCAD---CYQQSDPVFNPTSSSTYKSLTCSAPQCSLLETSACRSNKCLYQVSYG 247

Query: 247 DGSFTTGELATETLSFGNSNSIPNLPIGCGHDNEGLFAGGAGLIGLGGGAISLSSQLKAS 306
           DGSFT GELAT+T++FGNS  I N+ +GCGHDNEGLF G AGL+GLGGG +S+++Q+KA+
Sbjct: 248 DGSFTVGELATDTVTFGNSGKINNVALGCGHDNEGLFTGAAGLLGLGGGVLSITNQMKAT 307

Query: 307 SFSYCLVNLDSDSSSTLEFNS-NMPSDSLTSPLVKNDRFHSYRYVKVVGISVGGKTLPIS 366
           SFSYCLV+ DS  SS+L+FNS  +     T+PL++N +  ++ YV + G SVGG+ + + 
Sbjct: 308 SFSYCLVDRDSGKSSSLDFNSVQLGGGDATAPLLRNKKIDTFYYVGLSGFSVGGEKVVLP 367

Query: 367 PTRFEIDESGLGGIIVDSGTIISRLPSDVYESLREAFVKLTSSLSP-APGISVFDTCYNF 426
              F++D SG GG+I+D GT ++RL +  Y SLR+AF+KLT +L   +  IS+FDTCY+F
Sbjct: 368 DAIFDVDASGSGGVILDCGTAVTRLQTQAYNSLRDAFLKLTVNLKKGSSSISLFDTCYDF 427

Query: 427 SGQSNVEVPTIAFVLSEGTSLRLPARNYLIMLDTAGTYCLAFIKTKSSLSIIGSFQQQGI 486
           S  S V+VPT+AF  + G SL LPA+NYLI +D +GT+C AF  T SSLSIIG+ QQQG 
Sbjct: 428 SSLSTVKVPTVAFHFTGGKSLDLPAKNYLIPVDDSGTFCFAFAPTSSSLSIIGNVQQQGT 487

Query: 487 RVSYDLTNSLVGFSTNKC 488
           R++YDL+ +++G S NKC
Sbjct: 488 RITYDLSKNVIGLSGNKC 500

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BLAST of CSPI01G03700 vs. ExPASy Swiss-Prot
Match: Q9LHE3 (Protein ASPARTIC PROTEASE IN GUARD CELL 2 OS=Arabidopsis thaliana OX=3702 GN=ASPG2 PE=2 SV=1)

HSP 1 Score: 343.6 bits (880), Expect = 3.7e-93
Identity = 181/435 (41.61%), Postives = 272/435 (62.53%), Query Frame = 0


Query: 60  NHSHL---PNSPFSLPLYPRLALHNPSYKDYNTLVRARLTRDAARVQFLNRNLERSLNGG 119
           N++H     +S ++L L  R    + +Y++++  + AR+ RD  RV  + R +   +   
Sbjct: 47  NNTHFSDESSSKYTLRLLHRDRFPSVTYRNHHHRLHARMRRDTDRVSAILRRISGKVIPS 106

Query: 120 THFGESINESLTGDSITAPVVSGQSKGSGAEYLAQIGVGQPVKLFYLVPDTGSDVTWLQC 179
           +     +N+        + +VSG  +GSG EY  +IGVG P +  Y+V D+GSD+ W+QC
Sbjct: 107 SDSRYEVND------FGSDIVSGMDQGSG-EYFVRIGVGSPPRDQYMVIDSGSDMVWVQC 166

Query: 180 QPCASENTCYKQFDPIFDPKSSSSYSPLSCNSQQCKLLDKANCNSDTCIYQVHYGDGSFT 239
           QPC     CYKQ DP+FDP  S SY+ +SC S  C  ++ + C+S  C Y+V YGDGS+T
Sbjct: 167 QPC---KLCYKQSDPVFDPAKSGSYTGVSCGSSVCDRIENSGCHSGGCRYEVMYGDGSYT 226

Query: 240 TGELATETLSFGNSNSIPNLPIGCGHDNEGLFAGGAGLIGLGGGAISLSSQLK---ASSF 299
            G LA ETL+F  +  + N+ +GCGH N G+F G AGL+G+GGG++S   QL      +F
Sbjct: 227 KGTLALETLTFAKT-VVRNVAMGCGHRNRGMFIGAAGLLGIGGGSMSFVGQLSGQTGGAF 286

Query: 300 SYCLVNLDSDSSSTLEFNSN-MPSDSLTSPLVKNDRFHSYRYVKVVGISVGGKTLPISPT 359
            YCLV+  +DS+ +L F    +P  +   PLV+N R  S+ YV + G+ VGG  +P+   
Sbjct: 287 GYCLVSRGTDSTGSLVFGREALPVGASWVPLVRNPRAPSFYYVGLKGLGVGGVRIPLPDG 346

Query: 360 RFEIDESGLGGIIVDSGTIISRLPSDVYESLREAFVKLTSSLSPAPGISVFDTCYNFSGQ 419
            F++ E+G GG+++D+GT ++RLP+  Y + R+ F   T++L  A G+S+FDTCY+ SG 
Sbjct: 347 VFDLTETGDGGVVMDTGTAVTRLPTAAYVAFRDGFKSQTANLPRASGVSIFDTCYDLSGF 406

Query: 420 SNVEVPTIAFVLSEGTSLRLPARNYLIMLDTAGTYCLAFIKTKSSLSIIGSFQQQGIRVS 479
            +V VPT++F  +EG  L LPARN+L+ +D +GTYC AF  + + LSIIG+ QQ+GI+VS
Sbjct: 407 VSVRVPTVSFYFTEGPVLTLPARNFLMPVDDSGTYCFAFAASPTGLSIIGNIQQEGIQVS 466

Query: 480 YDLTNSLVGFSTNKC 488
           +D  N  VGF  N C
Sbjct: 467 FDGANGFVGFGPNVC 470

[Close]

BLAST of CSPI01G03700 vs. ExPASy Swiss-Prot
Match: Q9LNJ3 (Aspartyl protease family protein 2 OS=Arabidopsis thaliana OX=3702 GN=APF2 PE=2 SV=1)

HSP 1 Score: 325.1 bits (832), Expect = 1.4e-87
Identity = 181/413 (43.83%), Postives = 254/413 (61.50%), Query Frame = 0


Query: 83  SYKDYNTLVRARLTRDAARVQFLNRNLERSLNGGTHFGESINESLTGDSITAPVVSGQSK 142
           S K  + L  +RL RD+ RV+ +   L   +      G ++  +      ++ VVSG S+
Sbjct: 84  SNKTPDELFSSRLQRDSRRVKSI-ATLAAQIP-----GRNVTHAPRPGGFSSSVVSGLSQ 143

Query: 143 GSGAEYLAQIGVGQPVKLFYLVPDTGSDVTWLQCQPCASENTCYKQFDPIFDPKSSSSYS 202
           GSG EY  ++GVG P +  Y+V DTGSD+ WLQC PC     CY Q DPIFDP+ S +Y+
Sbjct: 144 GSG-EYFTRLGVGTPARYVYMVLDTGSDIVWLQCAPC---RRCYSQSDPIFDPRKSKTYA 203

Query: 203 PLSCNSQQCKLLDKANCNS--DTCIYQVHYGDGSFTTGELATETLSFGNSNSIPNLPIGC 262
            + C+S  C+ LD A CN+   TC+YQV YGDGSFT G+ +TETL+F   N +  + +GC
Sbjct: 204 TIPCSSPHCRRLDSAGCNTRRKTCLYQVSYGDGSFTVGDFSTETLTF-RRNRVKGVALGC 263

Query: 263 GHDNEGLFAGGAGLIGLGGGAISLSSQLK---ASSFSYCLVNLDSDS--SSTLEFNSNMP 322
           GHDNEGLF G AGL+GLG G +S   Q        FSYCLV+  + S  SS +  N+ + 
Sbjct: 264 GHDNEGLFVGAAGLLGLGKGKLSFPGQTGHRFNQKFSYCLVDRSASSKPSSVVFGNAAVS 323

Query: 323 SDSLTSPLVKNDRFHSYRYVKVVGISVGGKTLP-ISPTRFEIDESGLGGIIVDSGTIISR 382
             +  +PL+ N +  ++ YV ++GISVGG  +P ++ + F++D+ G GG+I+DSGT ++R
Sbjct: 324 RIARFTPLLSNPKLDTFYYVGLLGISVGGTRVPGVTASLFKLDQIGNGGVIIDSGTSVTR 383

Query: 383 LPSDVYESLREAFVKLTSSLSPAPGISVFDTCYNFSGQSNVEVPTIAFVLSEGTSLRLPA 442
           L    Y ++R+AF     +L  AP  S+FDTC++ S  + V+VPT+      G  + LPA
Sbjct: 384 LIRPAYIAMRDAFRVGAKTLKRAPDFSLFDTCFDLSNMNEVKVPTVVLHF-RGADVSLPA 443

Query: 443 RNYLIMLDTAGTYCLAFIKTKSSLSIIGSFQQQGIRVSYDLTNSLVGFSTNKC 488
            NYLI +DT G +C AF  T   LSIIG+ QQQG RV YDL +S VGF+   C
Sbjct: 444 TNYLIPVDTNGKFCFAFAGTMGGLSIIGNIQQQGFRVVYDLASSRVGFAPGGC 484

[Close]

BLAST of CSPI01G03700 vs. ExPASy Swiss-Prot
Match: Q766C3 (Aspartic proteinase nepenthesin-1 OS=Nepenthes gracilis OX=150966 GN=nep1 PE=1 SV=1)

HSP 1 Score: 258.1 bits (658), Expect = 2.0e-67
Identity = 152/387 (39.28%), Postives = 225/387 (58.14%), Query Frame = 0


Query: 109 LERSLNGGTHFGESINESLTGDS-ITAPVVSGQSKGSGAEYLAQIGVGQPVKLFYLVPDT 168
           LER++  G+   + +   L G S +   V +G       EYL  + +G P + F  + DT
Sbjct: 60  LERAIERGSRRLQRLEAMLNGPSGVETSVYAGD-----GEYLMNLSIGTPAQPFSAIMDT 119

Query: 169 GSDVTWLQCQPCASENTCYKQFDPIFDPKSSSSYSPLSCNSQQCKLLDKANCNSDTCIYQ 228
           GSD+ W QCQPC     C+ Q  PIF+P+ SSS+S L C+SQ C+ L    C+++ C Y 
Sbjct: 120 GSDLIWTQCQPCTQ---CFNQSTPIFNPQGSSSFSTLPCSSQLCQALSSPTCSNNFCQYT 179

Query: 229 VHYGDGSFTTGELATETLSFGNSNSIPNLPIGCGHDNEGLFAG-GAGLIGLGGGAISLSS 288
             YGDGS T G + TETL+FG S SIPN+  GCG +N+G   G GAGL+G+G G +SL S
Sbjct: 180 YGYGDGSETQGSMGTETLTFG-SVSIPNITFGCGENNQGFGQGNGAGLVGMGRGPLSLPS 239

Query: 289 QLKASSFSYCLVNLDSDSSSTLEFNSNMPSDSLTSP---LVKNDRFHSYRYVKVVGISVG 348
           QL  + FSYC+  + S + S L   S   S +  SP   L+++ +  ++ Y+ + G+SVG
Sbjct: 240 QLDVTKFSYCMTPIGSSTPSNLLLGSLANSVTAGSPNTTLIQSSQIPTFYYITLNGLSVG 299

Query: 349 GKTLPISPTRFEID-ESGLGGIIVDSGTIISRLPSDVYESLREAFVKLTSSLSPAPGISV 408
              LPI P+ F ++  +G GGII+DSGT ++   ++ Y+S+R+ F+   +        S 
Sbjct: 300 STRLPIDPSAFALNSNNGTGGIIIDSGTTLTYFVNNAYQSVRQEFISQINLPVVNGSSSG 359

Query: 409 FDTCYNF-SGQSNVEVPTIAFVLS-EGTSLRLPARNYLIMLDTAGTYCLAFIKTKSSLSI 468
           FD C+   S  SN+++PT  FV+  +G  L LP+ NY I   + G  CLA   +   +SI
Sbjct: 360 FDLCFQTPSDPSNLQIPT--FVMHFDGGDLELPSENYFIS-PSNGLICLAMGSSSQGMSI 419

Query: 469 IGSFQQQGIRVSYDLTNSLVGFSTNKC 488
            G+ QQQ + V YD  NS+V F++ +C
Sbjct: 420 FGNIQQQNMLVVYDTGNSVVSFASAQC 434

[Close]

BLAST of CSPI01G03700 vs. ExPASy Swiss-Prot
Match: Q9LEW3 (Aspartyl protease AED1 OS=Arabidopsis thaliana OX=3702 GN=AED1 PE=2 SV=1)

HSP 1 Score: 251.5 bits (641), Expect = 1.9e-65
Identity = 156/406 (38.42%), Postives = 223/406 (54.93%), Query Frame = 0


Query: 86  DYNTLVRARLTRDAARVQFLNRNLERSLNGGTHFGESINESLTGDSITAPVVSGQSKGSG 145
           D++ ++R    RD ARV+ +   L ++         S NE     S   P  SG + GSG
Sbjct: 84  DHDEIIR----RDQARVESIYSKLSKN---------SANEVSEAKSTELPAKSGITLGSG 143

Query: 146 AEYLAQIGVGQPVKLFYLVPDTGSDVTWLQCQPCASENTCYKQFDPIFDPKSSSSYSPLS 205
             Y+  IG+G P     LV DTGSD+TW QC+PC    +CY Q +P F+P SSS+Y  +S
Sbjct: 144 -NYIVTIGIGTPKHDLSLVFDTGSDLTWTQCEPCL--GSCYSQKEPKFNPSSSSTYQNVS 203

Query: 206 CNSQQCKLLDKANCNSDTCIYQVHYGDGSFTTGELATETLSFGNSNSIPNLPIGCGHDNE 265
           C+S  C+  D  +C++  C+Y + YGD SFT G LA E  +  NS+ + ++  GCG +N+
Sbjct: 204 CSSPMCE--DAESCSASNCVYSIVYGDKSFTQGFLAKEKFTLTNSDVLEDVYFGCGENNQ 263

Query: 266 GLFAGGAGLIGLGGGAISLSSQLKA---SSFSYCLVNLDSDSSSTLEFNSNMPSDSLT-S 325
           GLF G AGL+GLG G +SL +Q      + FSYCL +  S+S+  L F S   S+S+  +
Sbjct: 264 GLFDGVAGLLGLGPGKLSLPAQTTTTYNNIFSYCLPSFTSNSTGHLTFGSAGISESVKFT 323

Query: 326 PLVKNDRFHSYRYVKVVGISVGGKTLPISPTRFEIDESGLGGIIVDSGTIISRLPSDVYE 385
           P+       +Y  + ++GISVG K L I+P  F  +     G I+DSGT+ +RLP+ VY 
Sbjct: 324 PISSFPSAFNYG-IDIIGISVGDKELAITPNSFSTE-----GAIIDSGTVFTRLPTKVYA 383

Query: 386 SLREAFVKLTSSLSPAPGISVFDTCYNFSGQSNVEVPTIAFVLSEGTSLRLPARNYLIML 445
            LR  F +  SS     G  +FDTCY+F+G   V  PTIAF  +  T + L      + +
Sbjct: 384 ELRSVFKEKMSSYKSTSGYGLFDTCYDFTGLDTVTYPTIAFSFAGSTVVELDGSGISLPI 443

Query: 446 DTAGTYCLAFIKTKSSLSIIGSFQQQGIRVSYDLTNSLVGFSTNKC 488
             +   CLAF       +I G+ QQ  + V YD+    VGF+ N C
Sbjct: 444 KIS-QVCLAFAGNDDLPAIFGNVQQTTLDVVYDVAGGRVGFAPNGC 464

[Close]

BLAST of CSPI01G03700 vs. ExPASy TrEMBL
Match: A0A0A0LPJ3 (Aspartic proteinase nepenthesin-1 OS=Cucumis sativus OX=3659 GN=Csa_1G022490 PE=4 SV=1)

HSP 1 Score: 953.7 bits (2464), Expect = 2.9e-274
Identity = 486/487 (99.79%), Postives = 486/487 (99.79%), Query Frame = 0


Query: 1   MNTSLSSVFLFLTIFTSLQFPSILSRKLTPSSYSTSIFDVSASTNQALDALSIKPKPLQN 60
           MNTSLSSVFLFLTIFTSLQFPSILSRKLTPSSYSTSIFDVSASTNQALDALSIKPKPLQN
Sbjct: 1   MNTSLSSVFLFLTIFTSLQFPSILSRKLTPSSYSTSIFDVSASTNQALDALSIKPKPLQN 60

Query: 61  HSHLPNSPFSLPLYPRLALHNPSYKDYNTLVRARLTRDAARVQFLNRNLERSLNGGTHFG 120
           HSHLPNSPFSLPLYPRLALHNPSYKDYNTLVRARLTRDAARVQFLNRNLERSLNGGTHFG
Sbjct: 61  HSHLPNSPFSLPLYPRLALHNPSYKDYNTLVRARLTRDAARVQFLNRNLERSLNGGTHFG 120

Query: 121 ESINESLTGDSITAPVVSGQSKGSGAEYLAQIGVGQPVKLFYLVPDTGSDVTWLQCQPCA 180
           ESINESL GDSITAPVVSGQSKGSGAEYLAQIGVGQPVKLFYLVPDTGSDVTWLQCQPCA
Sbjct: 121 ESINESLIGDSITAPVVSGQSKGSGAEYLAQIGVGQPVKLFYLVPDTGSDVTWLQCQPCA 180

Query: 181 SENTCYKQFDPIFDPKSSSSYSPLSCNSQQCKLLDKANCNSDTCIYQVHYGDGSFTTGEL 240
           SENTCYKQFDPIFDPKSSSSYSPLSCNSQQCKLLDKANCNSDTCIYQVHYGDGSFTTGEL
Sbjct: 181 SENTCYKQFDPIFDPKSSSSYSPLSCNSQQCKLLDKANCNSDTCIYQVHYGDGSFTTGEL 240

Query: 241 ATETLSFGNSNSIPNLPIGCGHDNEGLFAGGAGLIGLGGGAISLSSQLKASSFSYCLVNL 300
           ATETLSFGNSNSIPNLPIGCGHDNEGLFAGGAGLIGLGGGAISLSSQLKASSFSYCLVNL
Sbjct: 241 ATETLSFGNSNSIPNLPIGCGHDNEGLFAGGAGLIGLGGGAISLSSQLKASSFSYCLVNL 300

Query: 301 DSDSSSTLEFNSNMPSDSLTSPLVKNDRFHSYRYVKVVGISVGGKTLPISPTRFEIDESG 360
           DSDSSSTLEFNSNMPSDSLTSPLVKNDRFHSYRYVKVVGISVGGKTLPISPTRFEIDESG
Sbjct: 301 DSDSSSTLEFNSNMPSDSLTSPLVKNDRFHSYRYVKVVGISVGGKTLPISPTRFEIDESG 360

Query: 361 LGGIIVDSGTIISRLPSDVYESLREAFVKLTSSLSPAPGISVFDTCYNFSGQSNVEVPTI 420
           LGGIIVDSGTIISRLPSDVYESLREAFVKLTSSLSPAPGISVFDTCYNFSGQSNVEVPTI
Sbjct: 361 LGGIIVDSGTIISRLPSDVYESLREAFVKLTSSLSPAPGISVFDTCYNFSGQSNVEVPTI 420

Query: 421 AFVLSEGTSLRLPARNYLIMLDTAGTYCLAFIKTKSSLSIIGSFQQQGIRVSYDLTNSLV 480
           AFVLSEGTSLRLPARNYLIMLDTAGTYCLAFIKTKSSLSIIGSFQQQGIRVSYDLTNSLV
Sbjct: 421 AFVLSEGTSLRLPARNYLIMLDTAGTYCLAFIKTKSSLSIIGSFQQQGIRVSYDLTNSLV 480

Query: 481 GFSTNKC 488
           GFSTNKC
Sbjct: 481 GFSTNKC 487

[Close]

BLAST of CSPI01G03700 vs. ExPASy TrEMBL
Match: A0A5A7UQC2 (Protein ASPARTIC PROTEASE IN GUARD CELL 1-like OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold274G003690 PE=4 SV=1)

HSP 1 Score: 876.3 bits (2263), Expect = 5.9e-251
Identity = 449/487 (92.20%), Postives = 460/487 (94.46%), Query Frame = 0


Query: 1   MNTSLSSVFLFLTIFTSLQFPSILSRKLTPSSYSTSIFDVSASTNQALDALSIKPKPLQN 60
           M TSLSSVFLFLTIFTSLQF SILSRKLT S YSTSIFDV ASTNQAL+ALSIKPK LQ 
Sbjct: 1   MKTSLSSVFLFLTIFTSLQFSSILSRKLTQSPYSTSIFDVLASTNQALNALSIKPKHLQT 60

Query: 61  HSHLPNSPFSLPLYPRLALHNPSYKDYNTLVRARLTRDAARVQFLNRNLERSLNGGTHFG 120
           HSHLPNS  SLPLYPRL+LHNPSYKDY++LVRARL RDAARVQFLNRNLE SLNGG  FG
Sbjct: 61  HSHLPNSSLSLPLYPRLSLHNPSYKDYDSLVRARLARDAARVQFLNRNLEHSLNGGKDFG 120

Query: 121 ESINESLTGDSITAPVVSGQSKGSGAEYLAQIGVGQPVKLFYLVPDTGSDVTWLQCQPCA 180
           E  N SL GDSITAPVVSGQSKGSGAEYLAQ+GVGQPVKLFYLVPDTGSDVTWLQCQPCA
Sbjct: 121 EVTNGSLIGDSITAPVVSGQSKGSGAEYLAQVGVGQPVKLFYLVPDTGSDVTWLQCQPCA 180

Query: 181 SENTCYKQFDPIFDPKSSSSYSPLSCNSQQCKLLDKANCNSDTCIYQVHYGDGSFTTGEL 240
           +EN CYKQ DPIFDPKSSSSY+PLSCNSQQC LLD+ NCNS TCIYQVHYGDGSFTTGEL
Sbjct: 181 TENACYKQIDPIFDPKSSSSYTPLSCNSQQCGLLDRPNCNSGTCIYQVHYGDGSFTTGEL 240

Query: 241 ATETLSFGNSNSIPNLPIGCGHDNEGLFAGGAGLIGLGGGAISLSSQLKASSFSYCLVNL 300
           ATETLSFGNSNSIPNLPIGCGHDNEGLFAGGAGLIGLGGGAISLSSQLKASSFSYCLVN+
Sbjct: 241 ATETLSFGNSNSIPNLPIGCGHDNEGLFAGGAGLIGLGGGAISLSSQLKASSFSYCLVNM 300

Query: 301 DSDSSSTLEFNSNMPSDSLTSPLVKNDRFHSYRYVKVVGISVGGKTLPISPTRFEIDESG 360
           DSDSSSTLEFNSNMPSDSLTSPLVKNDRFHSYRYVKV+GISVGGKTLPIS TRFEIDESG
Sbjct: 301 DSDSSSTLEFNSNMPSDSLTSPLVKNDRFHSYRYVKVIGISVGGKTLPISSTRFEIDESG 360

Query: 361 LGGIIVDSGTIISRLPSDVYESLREAFVKLTSSLSPAPGISVFDTCYNFSGQSNVEVPTI 420
           LGGIIVDSGTIISRLPSDVYESLREAFVKLTSSLSPAPGISVFDTCYN S QSNVEVPTI
Sbjct: 361 LGGIIVDSGTIISRLPSDVYESLREAFVKLTSSLSPAPGISVFDTCYNLSSQSNVEVPTI 420

Query: 421 AFVLSEGTSLRLPARNYLIMLDTAGTYCLAFIKTKSSLSIIGSFQQQGIRVSYDLTNSLV 480
           AFVLS GTSLRLPARNYLI +DTAGTYCLAFIKTKSSLSIIGSFQQQGIRVSYDLTNSLV
Sbjct: 421 AFVLSGGTSLRLPARNYLIRVDTAGTYCLAFIKTKSSLSIIGSFQQQGIRVSYDLTNSLV 480

Query: 481 GFSTNKC 488
           GFSTNKC
Sbjct: 481 GFSTNKC 487

[Close]

BLAST of CSPI01G03700 vs. ExPASy TrEMBL
Match: A0A5D3DYG2 (Protein ASPARTIC PROTEASE IN GUARD CELL 1-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold629G00370 PE=4 SV=1)

HSP 1 Score: 874.8 bits (2259), Expect = 1.7e-250
Identity = 449/487 (92.20%), Postives = 459/487 (94.25%), Query Frame = 0


Query: 1   MNTSLSSVFLFLTIFTSLQFPSILSRKLTPSSYSTSIFDVSASTNQALDALSIKPKPLQN 60
           M TSLSSVFLFLTIFTSLQF SILSRKLT S YSTSIFDV ASTNQAL+ALSIKPK LQ 
Sbjct: 1   MKTSLSSVFLFLTIFTSLQFSSILSRKLTQSPYSTSIFDVLASTNQALNALSIKPKHLQT 60

Query: 61  HSHLPNSPFSLPLYPRLALHNPSYKDYNTLVRARLTRDAARVQFLNRNLERSLNGGTHFG 120
           HSHLPNS  SLPLYPRL+LHNPSYKDY++LVRARL RDAARVQFLNRNLE SLNGG  FG
Sbjct: 61  HSHLPNSSLSLPLYPRLSLHNPSYKDYDSLVRARLARDAARVQFLNRNLEHSLNGGKDFG 120

Query: 121 ESINESLTGDSITAPVVSGQSKGSGAEYLAQIGVGQPVKLFYLVPDTGSDVTWLQCQPCA 180
           E  N SL GDSITAPVVSGQSKGSGAEYLAQ+GVGQPVKLFYLVPDTGSDVTWLQCQPCA
Sbjct: 121 EVTNGSLIGDSITAPVVSGQSKGSGAEYLAQVGVGQPVKLFYLVPDTGSDVTWLQCQPCA 180

Query: 181 SENTCYKQFDPIFDPKSSSSYSPLSCNSQQCKLLDKANCNSDTCIYQVHYGDGSFTTGEL 240
           +EN CYKQ DPIFDPKSSSSY+PLSCNSQQC LLD+ NCNS TCIYQVHYGDGSFTTGEL
Sbjct: 181 TENACYKQIDPIFDPKSSSSYTPLSCNSQQCGLLDRPNCNSGTCIYQVHYGDGSFTTGEL 240

Query: 241 ATETLSFGNSNSIPNLPIGCGHDNEGLFAGGAGLIGLGGGAISLSSQLKASSFSYCLVNL 300
           ATE LSFGNSNSIPNLPIGCGHDNEGLFAGGAGLIGLGGGAISLSSQLKASSFSYCLVN+
Sbjct: 241 ATEILSFGNSNSIPNLPIGCGHDNEGLFAGGAGLIGLGGGAISLSSQLKASSFSYCLVNM 300

Query: 301 DSDSSSTLEFNSNMPSDSLTSPLVKNDRFHSYRYVKVVGISVGGKTLPISPTRFEIDESG 360
           DSDSSSTLEFNSNMPSDSLTSPLVKNDRFHSYRYVKVVGISVGG TLPIS TRFEIDESG
Sbjct: 301 DSDSSSTLEFNSNMPSDSLTSPLVKNDRFHSYRYVKVVGISVGGNTLPISSTRFEIDESG 360

Query: 361 LGGIIVDSGTIISRLPSDVYESLREAFVKLTSSLSPAPGISVFDTCYNFSGQSNVEVPTI 420
           LGGIIVDSGTIISRLPSDVYESLREAFVKLTSSLSPAPGISVFDTCYN SGQSNVEVPTI
Sbjct: 361 LGGIIVDSGTIISRLPSDVYESLREAFVKLTSSLSPAPGISVFDTCYNLSGQSNVEVPTI 420

Query: 421 AFVLSEGTSLRLPARNYLIMLDTAGTYCLAFIKTKSSLSIIGSFQQQGIRVSYDLTNSLV 480
           AFVLS GTSLRLPARNYLI +DTAGTYCLAFIKTKSSLSIIGSFQQQGIRVSYDLTNSLV
Sbjct: 421 AFVLSGGTSLRLPARNYLIRVDTAGTYCLAFIKTKSSLSIIGSFQQQGIRVSYDLTNSLV 480

Query: 481 GFSTNKC 488
           GFSTNKC
Sbjct: 481 GFSTNKC 487

[Close]

BLAST of CSPI01G03700 vs. ExPASy TrEMBL
Match: A0A1S4E062 (protein ASPARTIC PROTEASE IN GUARD CELL 1-like OS=Cucumis melo OX=3656 GN=LOC103494117 PE=4 SV=1)

HSP 1 Score: 815.8 bits (2106), Expect = 9.4e-233
Identity = 425/487 (87.27%), Postives = 436/487 (89.53%), Query Frame = 0


Query: 1   MNTSLSSVFLFLTIFTSLQFPSILSRKLTPSSYSTSIFDVSASTNQALDALSIKPKPLQN 60
           M TSLSSVFLFLTIFTSLQF SILSRKLT S YSTSIFDV ASTNQAL+ALSIKPK LQ 
Sbjct: 1   MKTSLSSVFLFLTIFTSLQFSSILSRKLTQSPYSTSIFDVLASTNQALNALSIKPKHLQT 60

Query: 61  HSHLPNSPFSLPLYPRLALHNPSYKDYNTLVRARLTRDAARVQFLNRNLERSLNGGTHFG 120
           HSHLPNS  SLPLYPRL+LHNPSYKDY++LVRARL RDAARVQFLNRNLE SLNGG  FG
Sbjct: 61  HSHLPNSSLSLPLYPRLSLHNPSYKDYDSLVRARLARDAARVQFLNRNLEHSLNGGKDFG 120

Query: 121 ESINESLTGDSITAPVVSGQSKGSGAEYLAQIGVGQPVKLFYLVPDTGSDVTWLQCQPCA 180
           E  N SL GDSITAPVVSGQSKGSGAEYLAQ+GVGQPVKLFYLVPDTGSDVTWLQCQPCA
Sbjct: 121 EVTNGSLIGDSITAPVVSGQSKGSGAEYLAQVGVGQPVKLFYLVPDTGSDVTWLQCQPCA 180

Query: 181 SENTCYKQFDPIFDPKSSSSYSPLSCNSQQCKLLDKANCNSDTCIYQVHYGDGSFTTGEL 240
           +EN CYKQ DPIFDPKSSSSY+PLSCNSQQC LLD+ NCNS TCIYQVHYGDGSFTTGEL
Sbjct: 181 TENACYKQIDPIFDPKSSSSYTPLSCNSQQCGLLDRPNCNSGTCIYQVHYGDGSFTTGEL 240

Query: 241 ATETLSFGNSNSIPNLPIGCGHDNEGLFAGGAGLIGLGGGAISLSSQLKASSFSYCLVNL 300
           ATETLSFGNSNSIPNLPIGCGHDNEGLFAGGAGLIGLGGGAISLSSQLKASSFSYCLVN+
Sbjct: 241 ATETLSFGNSNSIPNLPIGCGHDNEGLFAGGAGLIGLGGGAISLSSQLKASSFSYCLVNM 300

Query: 301 DSDSSSTLEFNSNMPSDSLTSPLVKNDRFHSYRYVKVVGISVGGKTLPISPTRFEIDESG 360
           DSDSSSTLEFNSNMPSDSLTSPLVKNDRFHSYRYVKV+GISVGGKTLPIS TRFEIDESG
Sbjct: 301 DSDSSSTLEFNSNMPSDSLTSPLVKNDRFHSYRYVKVIGISVGGKTLPISSTRFEIDESG 360

Query: 361 LGGIIVDSGTIISRLPSDVYESLREAFVKLTSSLSPAPGISVFDTCYNFSGQSNVEVPTI 420
           LGGIIVDSGTIISRLPSDVYESLREAFVKLTSSLSPAPG                     
Sbjct: 361 LGGIIVDSGTIISRLPSDVYESLREAFVKLTSSLSPAPG--------------------- 420

Query: 421 AFVLSEGTSLRLPARNYLIMLDTAGTYCLAFIKTKSSLSIIGSFQQQGIRVSYDLTNSLV 480
                 GTSLRLPARNYLI +DTAGTYCLAFIKTKSSLSIIGSFQQQGIRVSYDLTNSLV
Sbjct: 421 ------GTSLRLPARNYLIRVDTAGTYCLAFIKTKSSLSIIGSFQQQGIRVSYDLTNSLV 460

Query: 481 GFSTNKC 488
           GFSTNKC
Sbjct: 481 GFSTNKC 460

[Close]

BLAST of CSPI01G03700 vs. ExPASy TrEMBL
Match: A0A5A7UQC3 (Protein ASPARTIC PROTEASE IN GUARD CELL 1 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold274G003680 PE=3 SV=1)

HSP 1 Score: 709.9 bits (1831), Expect = 7.3e-201
Identity = 365/488 (74.80%), Postives = 416/488 (85.25%), Query Frame = 0


Query: 1   MNTSLSSVFLFLTIFTSLQFPSILSRKLTPSS-YSTSIFDVSASTNQALDALSIKPKPLQ 60
           MNTSLS   LFLTIFT LQFPSILSRKLT  S YST+ FDVSAS NQAL+ALSIKPKP Q
Sbjct: 2   MNTSLSYALLFLTIFTFLQFPSILSRKLTAQSPYSTTTFDVSASINQALNALSIKPKPFQ 61

Query: 61  NHSHLPNSPFSLPLYPRLALHNPSYKDYNTLVRARLTRDAARVQFLNRNLERSLNGGTHF 120
            HS+  NSP SL L+PRL +HNPSYKDY TLVRARL R A RVQ LNR LE SLNG   F
Sbjct: 62  THSYHSNSPLSLSLHPRLTVHNPSYKDYGTLVRARLARHATRVQSLNRKLELSLNGAKQF 121

Query: 121 GESINESLTGDSITAPVVSGQSKGSGAEYLAQIGVGQPVKLFYLVPDTGSDVTWLQCQPC 180
           G+ IN S + +S+TAPV SG S G G EY A+IGVGQPV+ F+LVPDTGSDVTWLQC+PC
Sbjct: 122 GKRINGSASTNSLTAPVTSGASHGDG-EYFARIGVGQPVQSFFLVPDTGSDVTWLQCKPC 181

Query: 181 ASENTCYKQFDPIFDPKSSSSYSPLSCNSQQCKLLDKANCNSDTCIYQVHYGDGSFTTGE 240
           A+EN C+KQ DPIFDPKSSSSYS LSCNS+QC+LLD+A C+S++CIY+V YGDGSFT GE
Sbjct: 182 ANENACFKQLDPIFDPKSSSSYSSLSCNSEQCQLLDEAGCSSNSCIYEVEYGDGSFTIGE 241

Query: 241 LATETLSFGNSNSIPNLPIGCGHDNEGLFAGGAGLIGLGGGAISLSSQLKASSFSYCLVN 300
           LATETLSFGNSNSIPNLPIGCGHDNEGLF   AGLIGLGGGAISLSSQL+ASSFSYCLV+
Sbjct: 242 LATETLSFGNSNSIPNLPIGCGHDNEGLFDAAAGLIGLGGGAISLSSQLQASSFSYCLVD 301

Query: 301 LDSDSSSTLEFNSNMPSDSLTSPLVKNDRFHSYRYVKVVGISVGGKTLPISPTRFEIDES 360
           LDSDSSSTL+FN++ PSDSLTSPLVKN+RF S+RYVKV+G+SVGGK LPIS +RFEIDES
Sbjct: 302 LDSDSSSTLDFNADQPSDSLTSPLVKNNRFPSFRYVKVIGMSVGGKRLPISSSRFEIDES 361

Query: 361 GLGGIIVDSGTIISRLPSDVYESLREAFVKLTSSLSPAPGISVFDTCYNFSGQSNVEVPT 420
           G GGIIVDSGT I++LPSDVY+ LR+AFV LT++L  APG+S FDTCY+ S QS+VEVP 
Sbjct: 362 GSGGIIVDSGTTITQLPSDVYDVLRDAFVGLTTNLPTAPGVSPFDTCYDLSSQSSVEVPI 421

Query: 421 IAFVLSEGTSLRLPARNYLIMLDTAGTYCLAFIKTKSSLSIIGSFQQQGIRVSYDLTNSL 480
           IAF+L  G SL+LPA+N LI +D+AGT+CLAF+     LSIIG+ QQQGIRVSYDL NS+
Sbjct: 422 IAFILPGGKSLKLPAKNCLIQVDSAGTFCLAFLPGTFPLSIIGNVQQQGIRVSYDLDNSI 481

Query: 481 VGFSTNKC 488
           VGF+TNKC
Sbjct: 482 VGFATNKC 488

[Close]

BLAST of CSPI01G03700 vs. NCBI nr
Match: XP_004138238.1 (protein ASPARTIC PROTEASE IN GUARD CELL 1 [Cucumis sativus] >KGN63810.1 hypothetical protein Csa_014284 [Cucumis sativus])

HSP 1 Score: 953.7 bits (2464), Expect = 6.0e-274
Identity = 486/487 (99.79%), Postives = 486/487 (99.79%), Query Frame = 0


Query: 1   MNTSLSSVFLFLTIFTSLQFPSILSRKLTPSSYSTSIFDVSASTNQALDALSIKPKPLQN 60
           MNTSLSSVFLFLTIFTSLQFPSILSRKLTPSSYSTSIFDVSASTNQALDALSIKPKPLQN
Sbjct: 1   MNTSLSSVFLFLTIFTSLQFPSILSRKLTPSSYSTSIFDVSASTNQALDALSIKPKPLQN 60

Query: 61  HSHLPNSPFSLPLYPRLALHNPSYKDYNTLVRARLTRDAARVQFLNRNLERSLNGGTHFG 120
           HSHLPNSPFSLPLYPRLALHNPSYKDYNTLVRARLTRDAARVQFLNRNLERSLNGGTHFG
Sbjct: 61  HSHLPNSPFSLPLYPRLALHNPSYKDYNTLVRARLTRDAARVQFLNRNLERSLNGGTHFG 120

Query: 121 ESINESLTGDSITAPVVSGQSKGSGAEYLAQIGVGQPVKLFYLVPDTGSDVTWLQCQPCA 180
           ESINESL GDSITAPVVSGQSKGSGAEYLAQIGVGQPVKLFYLVPDTGSDVTWLQCQPCA
Sbjct: 121 ESINESLIGDSITAPVVSGQSKGSGAEYLAQIGVGQPVKLFYLVPDTGSDVTWLQCQPCA 180

Query: 181 SENTCYKQFDPIFDPKSSSSYSPLSCNSQQCKLLDKANCNSDTCIYQVHYGDGSFTTGEL 240
           SENTCYKQFDPIFDPKSSSSYSPLSCNSQQCKLLDKANCNSDTCIYQVHYGDGSFTTGEL
Sbjct: 181 SENTCYKQFDPIFDPKSSSSYSPLSCNSQQCKLLDKANCNSDTCIYQVHYGDGSFTTGEL 240

Query: 241 ATETLSFGNSNSIPNLPIGCGHDNEGLFAGGAGLIGLGGGAISLSSQLKASSFSYCLVNL 300
           ATETLSFGNSNSIPNLPIGCGHDNEGLFAGGAGLIGLGGGAISLSSQLKASSFSYCLVNL
Sbjct: 241 ATETLSFGNSNSIPNLPIGCGHDNEGLFAGGAGLIGLGGGAISLSSQLKASSFSYCLVNL 300

Query: 301 DSDSSSTLEFNSNMPSDSLTSPLVKNDRFHSYRYVKVVGISVGGKTLPISPTRFEIDESG 360
           DSDSSSTLEFNSNMPSDSLTSPLVKNDRFHSYRYVKVVGISVGGKTLPISPTRFEIDESG
Sbjct: 301 DSDSSSTLEFNSNMPSDSLTSPLVKNDRFHSYRYVKVVGISVGGKTLPISPTRFEIDESG 360

Query: 361 LGGIIVDSGTIISRLPSDVYESLREAFVKLTSSLSPAPGISVFDTCYNFSGQSNVEVPTI 420
           LGGIIVDSGTIISRLPSDVYESLREAFVKLTSSLSPAPGISVFDTCYNFSGQSNVEVPTI
Sbjct: 361 LGGIIVDSGTIISRLPSDVYESLREAFVKLTSSLSPAPGISVFDTCYNFSGQSNVEVPTI 420

Query: 421 AFVLSEGTSLRLPARNYLIMLDTAGTYCLAFIKTKSSLSIIGSFQQQGIRVSYDLTNSLV 480
           AFVLSEGTSLRLPARNYLIMLDTAGTYCLAFIKTKSSLSIIGSFQQQGIRVSYDLTNSLV
Sbjct: 421 AFVLSEGTSLRLPARNYLIMLDTAGTYCLAFIKTKSSLSIIGSFQQQGIRVSYDLTNSLV 480

Query: 481 GFSTNKC 488
           GFSTNKC
Sbjct: 481 GFSTNKC 487

[Close]

BLAST of CSPI01G03700 vs. NCBI nr
Match: KAA0058063.1 (protein ASPARTIC PROTEASE IN GUARD CELL 1-like [Cucumis melo var. makuwa])

HSP 1 Score: 876.3 bits (2263), Expect = 1.2e-250
Identity = 449/487 (92.20%), Postives = 460/487 (94.46%), Query Frame = 0


Query: 1   MNTSLSSVFLFLTIFTSLQFPSILSRKLTPSSYSTSIFDVSASTNQALDALSIKPKPLQN 60
           M TSLSSVFLFLTIFTSLQF SILSRKLT S YSTSIFDV ASTNQAL+ALSIKPK LQ 
Sbjct: 1   MKTSLSSVFLFLTIFTSLQFSSILSRKLTQSPYSTSIFDVLASTNQALNALSIKPKHLQT 60

Query: 61  HSHLPNSPFSLPLYPRLALHNPSYKDYNTLVRARLTRDAARVQFLNRNLERSLNGGTHFG 120
           HSHLPNS  SLPLYPRL+LHNPSYKDY++LVRARL RDAARVQFLNRNLE SLNGG  FG
Sbjct: 61  HSHLPNSSLSLPLYPRLSLHNPSYKDYDSLVRARLARDAARVQFLNRNLEHSLNGGKDFG 120

Query: 121 ESINESLTGDSITAPVVSGQSKGSGAEYLAQIGVGQPVKLFYLVPDTGSDVTWLQCQPCA 180
           E  N SL GDSITAPVVSGQSKGSGAEYLAQ+GVGQPVKLFYLVPDTGSDVTWLQCQPCA
Sbjct: 121 EVTNGSLIGDSITAPVVSGQSKGSGAEYLAQVGVGQPVKLFYLVPDTGSDVTWLQCQPCA 180

Query: 181 SENTCYKQFDPIFDPKSSSSYSPLSCNSQQCKLLDKANCNSDTCIYQVHYGDGSFTTGEL 240
           +EN CYKQ DPIFDPKSSSSY+PLSCNSQQC LLD+ NCNS TCIYQVHYGDGSFTTGEL
Sbjct: 181 TENACYKQIDPIFDPKSSSSYTPLSCNSQQCGLLDRPNCNSGTCIYQVHYGDGSFTTGEL 240

Query: 241 ATETLSFGNSNSIPNLPIGCGHDNEGLFAGGAGLIGLGGGAISLSSQLKASSFSYCLVNL 300
           ATETLSFGNSNSIPNLPIGCGHDNEGLFAGGAGLIGLGGGAISLSSQLKASSFSYCLVN+
Sbjct: 241 ATETLSFGNSNSIPNLPIGCGHDNEGLFAGGAGLIGLGGGAISLSSQLKASSFSYCLVNM 300

Query: 301 DSDSSSTLEFNSNMPSDSLTSPLVKNDRFHSYRYVKVVGISVGGKTLPISPTRFEIDESG 360
           DSDSSSTLEFNSNMPSDSLTSPLVKNDRFHSYRYVKV+GISVGGKTLPIS TRFEIDESG
Sbjct: 301 DSDSSSTLEFNSNMPSDSLTSPLVKNDRFHSYRYVKVIGISVGGKTLPISSTRFEIDESG 360

Query: 361 LGGIIVDSGTIISRLPSDVYESLREAFVKLTSSLSPAPGISVFDTCYNFSGQSNVEVPTI 420
           LGGIIVDSGTIISRLPSDVYESLREAFVKLTSSLSPAPGISVFDTCYN S QSNVEVPTI
Sbjct: 361 LGGIIVDSGTIISRLPSDVYESLREAFVKLTSSLSPAPGISVFDTCYNLSSQSNVEVPTI 420

Query: 421 AFVLSEGTSLRLPARNYLIMLDTAGTYCLAFIKTKSSLSIIGSFQQQGIRVSYDLTNSLV 480
           AFVLS GTSLRLPARNYLI +DTAGTYCLAFIKTKSSLSIIGSFQQQGIRVSYDLTNSLV
Sbjct: 421 AFVLSGGTSLRLPARNYLIRVDTAGTYCLAFIKTKSSLSIIGSFQQQGIRVSYDLTNSLV 480

Query: 481 GFSTNKC 488
           GFSTNKC
Sbjct: 481 GFSTNKC 487

[Close]

BLAST of CSPI01G03700 vs. NCBI nr
Match: TYK28409.1 (protein ASPARTIC PROTEASE IN GUARD CELL 1-like [Cucumis melo var. makuwa])

HSP 1 Score: 874.8 bits (2259), Expect = 3.5e-250
Identity = 449/487 (92.20%), Postives = 459/487 (94.25%), Query Frame = 0


Query: 1   MNTSLSSVFLFLTIFTSLQFPSILSRKLTPSSYSTSIFDVSASTNQALDALSIKPKPLQN 60
           M TSLSSVFLFLTIFTSLQF SILSRKLT S YSTSIFDV ASTNQAL+ALSIKPK LQ 
Sbjct: 1   MKTSLSSVFLFLTIFTSLQFSSILSRKLTQSPYSTSIFDVLASTNQALNALSIKPKHLQT 60

Query: 61  HSHLPNSPFSLPLYPRLALHNPSYKDYNTLVRARLTRDAARVQFLNRNLERSLNGGTHFG 120
           HSHLPNS  SLPLYPRL+LHNPSYKDY++LVRARL RDAARVQFLNRNLE SLNGG  FG
Sbjct: 61  HSHLPNSSLSLPLYPRLSLHNPSYKDYDSLVRARLARDAARVQFLNRNLEHSLNGGKDFG 120

Query: 121 ESINESLTGDSITAPVVSGQSKGSGAEYLAQIGVGQPVKLFYLVPDTGSDVTWLQCQPCA 180
           E  N SL GDSITAPVVSGQSKGSGAEYLAQ+GVGQPVKLFYLVPDTGSDVTWLQCQPCA
Sbjct: 121 EVTNGSLIGDSITAPVVSGQSKGSGAEYLAQVGVGQPVKLFYLVPDTGSDVTWLQCQPCA 180

Query: 181 SENTCYKQFDPIFDPKSSSSYSPLSCNSQQCKLLDKANCNSDTCIYQVHYGDGSFTTGEL 240
           +EN CYKQ DPIFDPKSSSSY+PLSCNSQQC LLD+ NCNS TCIYQVHYGDGSFTTGEL
Sbjct: 181 TENACYKQIDPIFDPKSSSSYTPLSCNSQQCGLLDRPNCNSGTCIYQVHYGDGSFTTGEL 240

Query: 241 ATETLSFGNSNSIPNLPIGCGHDNEGLFAGGAGLIGLGGGAISLSSQLKASSFSYCLVNL 300
           ATE LSFGNSNSIPNLPIGCGHDNEGLFAGGAGLIGLGGGAISLSSQLKASSFSYCLVN+
Sbjct: 241 ATEILSFGNSNSIPNLPIGCGHDNEGLFAGGAGLIGLGGGAISLSSQLKASSFSYCLVNM 300

Query: 301 DSDSSSTLEFNSNMPSDSLTSPLVKNDRFHSYRYVKVVGISVGGKTLPISPTRFEIDESG 360
           DSDSSSTLEFNSNMPSDSLTSPLVKNDRFHSYRYVKVVGISVGG TLPIS TRFEIDESG
Sbjct: 301 DSDSSSTLEFNSNMPSDSLTSPLVKNDRFHSYRYVKVVGISVGGNTLPISSTRFEIDESG 360

Query: 361 LGGIIVDSGTIISRLPSDVYESLREAFVKLTSSLSPAPGISVFDTCYNFSGQSNVEVPTI 420
           LGGIIVDSGTIISRLPSDVYESLREAFVKLTSSLSPAPGISVFDTCYN SGQSNVEVPTI
Sbjct: 361 LGGIIVDSGTIISRLPSDVYESLREAFVKLTSSLSPAPGISVFDTCYNLSGQSNVEVPTI 420

Query: 421 AFVLSEGTSLRLPARNYLIMLDTAGTYCLAFIKTKSSLSIIGSFQQQGIRVSYDLTNSLV 480
           AFVLS GTSLRLPARNYLI +DTAGTYCLAFIKTKSSLSIIGSFQQQGIRVSYDLTNSLV
Sbjct: 421 AFVLSGGTSLRLPARNYLIRVDTAGTYCLAFIKTKSSLSIIGSFQQQGIRVSYDLTNSLV 480

Query: 481 GFSTNKC 488
           GFSTNKC
Sbjct: 481 GFSTNKC 487

[Close]

BLAST of CSPI01G03700 vs. NCBI nr
Match: XP_016901370.1 (PREDICTED: protein ASPARTIC PROTEASE IN GUARD CELL 1-like [Cucumis melo])

HSP 1 Score: 815.8 bits (2106), Expect = 1.9e-232
Identity = 425/487 (87.27%), Postives = 436/487 (89.53%), Query Frame = 0


Query: 1   MNTSLSSVFLFLTIFTSLQFPSILSRKLTPSSYSTSIFDVSASTNQALDALSIKPKPLQN 60
           M TSLSSVFLFLTIFTSLQF SILSRKLT S YSTSIFDV ASTNQAL+ALSIKPK LQ 
Sbjct: 1   MKTSLSSVFLFLTIFTSLQFSSILSRKLTQSPYSTSIFDVLASTNQALNALSIKPKHLQT 60

Query: 61  HSHLPNSPFSLPLYPRLALHNPSYKDYNTLVRARLTRDAARVQFLNRNLERSLNGGTHFG 120
           HSHLPNS  SLPLYPRL+LHNPSYKDY++LVRARL RDAARVQFLNRNLE SLNGG  FG
Sbjct: 61  HSHLPNSSLSLPLYPRLSLHNPSYKDYDSLVRARLARDAARVQFLNRNLEHSLNGGKDFG 120

Query: 121 ESINESLTGDSITAPVVSGQSKGSGAEYLAQIGVGQPVKLFYLVPDTGSDVTWLQCQPCA 180
           E  N SL GDSITAPVVSGQSKGSGAEYLAQ+GVGQPVKLFYLVPDTGSDVTWLQCQPCA
Sbjct: 121 EVTNGSLIGDSITAPVVSGQSKGSGAEYLAQVGVGQPVKLFYLVPDTGSDVTWLQCQPCA 180

Query: 181 SENTCYKQFDPIFDPKSSSSYSPLSCNSQQCKLLDKANCNSDTCIYQVHYGDGSFTTGEL 240
           +EN CYKQ DPIFDPKSSSSY+PLSCNSQQC LLD+ NCNS TCIYQVHYGDGSFTTGEL
Sbjct: 181 TENACYKQIDPIFDPKSSSSYTPLSCNSQQCGLLDRPNCNSGTCIYQVHYGDGSFTTGEL 240

Query: 241 ATETLSFGNSNSIPNLPIGCGHDNEGLFAGGAGLIGLGGGAISLSSQLKASSFSYCLVNL 300
           ATETLSFGNSNSIPNLPIGCGHDNEGLFAGGAGLIGLGGGAISLSSQLKASSFSYCLVN+
Sbjct: 241 ATETLSFGNSNSIPNLPIGCGHDNEGLFAGGAGLIGLGGGAISLSSQLKASSFSYCLVNM 300

Query: 301 DSDSSSTLEFNSNMPSDSLTSPLVKNDRFHSYRYVKVVGISVGGKTLPISPTRFEIDESG 360
           DSDSSSTLEFNSNMPSDSLTSPLVKNDRFHSYRYVKV+GISVGGKTLPIS TRFEIDESG
Sbjct: 301 DSDSSSTLEFNSNMPSDSLTSPLVKNDRFHSYRYVKVIGISVGGKTLPISSTRFEIDESG 360

Query: 361 LGGIIVDSGTIISRLPSDVYESLREAFVKLTSSLSPAPGISVFDTCYNFSGQSNVEVPTI 420
           LGGIIVDSGTIISRLPSDVYESLREAFVKLTSSLSPAPG                     
Sbjct: 361 LGGIIVDSGTIISRLPSDVYESLREAFVKLTSSLSPAPG--------------------- 420

Query: 421 AFVLSEGTSLRLPARNYLIMLDTAGTYCLAFIKTKSSLSIIGSFQQQGIRVSYDLTNSLV 480
                 GTSLRLPARNYLI +DTAGTYCLAFIKTKSSLSIIGSFQQQGIRVSYDLTNSLV
Sbjct: 421 ------GTSLRLPARNYLIRVDTAGTYCLAFIKTKSSLSIIGSFQQQGIRVSYDLTNSLV 460

Query: 481 GFSTNKC 488
           GFSTNKC
Sbjct: 481 GFSTNKC 460

[Close]

BLAST of CSPI01G03700 vs. NCBI nr
Match: XP_038878113.1 (protein ASPARTIC PROTEASE IN GUARD CELL 1 [Benincasa hispida])

HSP 1 Score: 785.8 bits (2028), Expect = 2.2e-223
Identity = 401/487 (82.34%), Postives = 431/487 (88.50%), Query Frame = 0


Query: 1   MNTSLSSVFLFLTIFTSLQFPSILSRKLTPSSYSTSIFDVSASTNQALDALSIKPKPLQN 60
           MN S+SS F+FLTI TSLQFPSI SRKLT S YST+IFDVSAST QA +ALSIKPKP + 
Sbjct: 1   MNISISSAFIFLTILTSLQFPSIFSRKLTQSPYSTAIFDVSASTKQAQNALSIKPKPFET 60

Query: 61  HSHLPNSPFSLPLYPRLALHNPSYKDYNTLVRARLTRDAARVQFLNRNLERSLNGGTHFG 120
           HSH PNSP SLPL+PRL L+NPSYKDY +LVRARL RDA RVQ LNRNLE SLNGG   G
Sbjct: 61  HSHHPNSPLSLPLHPRLTLYNPSYKDYGSLVRARLARDAVRVQSLNRNLELSLNGGQKIG 120

Query: 121 ESINESLTGDSITAPVVSGQSKGSGAEYLAQIGVGQPVKLFYLVPDTGSDVTWLQCQPCA 180
             IN S + DSITAPVVSGQS G+G EY A+IGVGQPV+ FYLVPDTGSDVTWLQCQPCA
Sbjct: 121 AGINGSESADSITAPVVSGQSLGNGGEYFARIGVGQPVQSFYLVPDTGSDVTWLQCQPCA 180

Query: 181 SENTCYKQFDPIFDPKSSSSYSPLSCNSQQCKLLDKANCNSDTCIYQVHYGDGSFTTGEL 240
           +E  CYKQ DPIFDPKSSSSY+ LSCNSQQC+LLDKANCNSD C YQV+YGDGSFTTGEL
Sbjct: 181 AEKACYKQIDPIFDPKSSSSYTALSCNSQQCQLLDKANCNSDVCQYQVNYGDGSFTTGEL 240

Query: 241 ATETLSFGNSNSIPNLPIGCGHDNEGLFAGGAGLIGLGGGAISLSSQLKASSFSYCLVNL 300
           ATETLSFGNSNSIPNLPIGCGHDNEGLF G AGLIGLGGGAISLSSQLKASSFSYCLV+L
Sbjct: 241 ATETLSFGNSNSIPNLPIGCGHDNEGLFVGAAGLIGLGGGAISLSSQLKASSFSYCLVDL 300

Query: 301 DSDSSSTLEFNSNMPSDSLTSPLVKNDRFHSYRYVKVVGISVGGKTLPISPTRFEIDESG 360
           DSDSSSTLEFN++ PSDSLTSPLVKNDRFHSYRYVKVVG+SVGG  LPIS TRFEIDESG
Sbjct: 301 DSDSSSTLEFNTDRPSDSLTSPLVKNDRFHSYRYVKVVGMSVGGNPLPISSTRFEIDESG 360

Query: 361 LGGIIVDSGTIISRLPSDVYESLREAFVKLTSSLSPAPGISVFDTCYNFSGQSNVEVPTI 420
           LGGIIVDSGT I++LPSDVYESLREAFVK TS+L PA GIS+FDTCY+ SGQS+VEVP I
Sbjct: 361 LGGIIVDSGTTITQLPSDVYESLREAFVKQTSNLPPAQGISLFDTCYDLSGQSSVEVPVI 420

Query: 421 AFVLSEGTSLRLPARNYLIMLDTAGTYCLAFIKTKSSLSIIGSFQQQGIRVSYDLTNSLV 480
           AFVL    SLRLPA+NYLI +D+ GTYCLAF KT+SSLSIIGSFQQQGIRVSYDL NSLV
Sbjct: 421 AFVLPGENSLRLPAKNYLIPVDSDGTYCLAFFKTRSSLSIIGSFQQQGIRVSYDLANSLV 480

Query: 481 GFSTNKC 488
           GFSTNKC
Sbjct: 481 GFSTNKC 487

[Close]

BLAST of CSPI01G03700 vs. TAIR 10
Match: AT1G25510.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 449.1 bits (1154), Expect = 4.4e-126
Identity = 235/485 (48.45%), Postives = 335/485 (69.07%), Query Frame = 0


Query: 7   SVFLFLTIFTSLQFPSILSRKL-TPSSYSTSIFDVSASTNQALDALSIKPKPLQNHSHLP 66
           S F F+   TS    S+ SR L   S+ +TSI +V+ S ++     S +    +  +H  
Sbjct: 6   SFFFFIFFLTS--HSSVFSRILPETSTTTTSILNVADSIHRTKYTSSFRLNQQEEQTHSA 65

Query: 67  NSPFSLPLYPRLALHNPSYKDYNTLVRARLTRDAARVQFLNRNLERSLNGGTHFG-ESIN 126
           +S FSL L+ R+++    + DY +L  ARL RD ARV+ L   L+ ++N  +    + I+
Sbjct: 66  SSSFSLQLHSRVSVRGTEHSDYKSLTLARLNRDTARVKSLITRLDLAINNISKADLKPIS 125

Query: 127 ESLTGD--SITAPVVSGQSKGSGAEYLAQIGVGQPVKLFYLVPDTGSDVTWLQCQPCASE 186
              T +   I AP++SG ++GSG EY  ++G+G+P +  Y+V DTGSDV WLQC PCA  
Sbjct: 126 TMYTTEEQDIEAPLISGTTQGSG-EYFTRVGIGKPAREVYMVLDTGSDVNWLQCTPCAD- 185

Query: 187 NTCYKQFDPIFDPKSSSSYSPLSCNSQQCKLLDKANCNSDTCIYQVHYGDGSFTTGELAT 246
             CY Q +PIF+P SSSSY PLSC++ QC  L+ + C + TC+Y+V YGDGS+T G+ AT
Sbjct: 186 --CYHQTEPIFEPSSSSSYEPLSCDTPQCNALEVSECRNATCLYEVSYGDGSYTVGDFAT 245

Query: 247 ETLSFGNSNSIPNLPIGCGHDNEGLFAGGAGLIGLGGGAISLSSQLKASSFSYCLVNLDS 306
           ETL+ G S  + N+ +GCGH NEGLF G AGL+GLGGG ++L SQL  +SFSYCLV+ DS
Sbjct: 246 ETLTIG-STLVQNVAVGCGHSNEGLFVGAAGLLGLGGGLLALPSQLNTTSFSYCLVDRDS 305

Query: 307 DSSSTLEFNSNMPSDSLTSPLVKNDRFHSYRYVKVVGISVGGKTLPISPTRFEIDESGLG 366
           DS+ST++F +++  D++ +PL++N +  ++ Y+ + GISVGG+ L I  + FE+DESG G
Sbjct: 306 DSASTVDFGTSLSPDAVVAPLLRNHQLDTFYYLGLTGISVGGELLQIPQSSFEMDESGSG 365

Query: 367 GIIVDSGTIISRLPSDVYESLREAFVKLTSSLSPAPGISVFDTCYNFSGQSNVEVPTIAF 426
           GII+DSGT ++RL +++Y SLR++FVK T  L  A G+++FDTCYN S ++ VEVPT+AF
Sbjct: 366 GIIIDSGTAVTRLQTEIYNSLRDSFVKGTLDLEKAAGVAMFDTCYNLSAKTTVEVPTVAF 425

Query: 427 VLSEGTSLRLPARNYLIMLDTAGTYCLAFIKTKSSLSIIGSFQQQGIRVSYDLTNSLVGF 486
               G  L LPA+NY+I +D+ GT+CLAF  T SSL+IIG+ QQQG RV++DL NSL+GF
Sbjct: 426 HFPGGKMLALPAKNYMIPVDSVGTFCLAFAPTASSLAIIGNVQQQGTRVTFDLANSLIGF 483

Query: 487 STNKC 488
           S+NKC
Sbjct: 486 SSNKC 483

[Close]

BLAST of CSPI01G03700 vs. TAIR 10
Match: AT3G18490.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 423.7 bits (1088), Expect = 2.0e-118
Identity = 234/498 (46.99%), Postives = 328/498 (65.86%), Query Frame = 0


Query: 7   SVFLFLTIFTSLQFPSILSRKLTPSSYSTSIFDVSASTNQALDALSI----------KPK 66
           S+   +T+   L      SR L+ +   T++ DV +S  Q    LS+          KP+
Sbjct: 8   SLLAVVTLSLFLTTTDASSRSLS-TPPKTNVLDVVSSLQQTQTILSLDPTRSSLTTTKPE 67

Query: 67  PLQNHSHL-PNSPFSLPLYPRLALHNPSYKDYNTLVRARLTRDAARVQFLNRNLERSLNG 126
            L +      +SP SL L+ R       +KDY +L  +RL RD++RV  +   +  ++ G
Sbjct: 68  SLSDPVFFNSSSPLSLELHSRDTFVASQHKDYKSLTLSRLERDSSRVAGIVAKIRFAVEG 127

Query: 127 --GTHFGESINES--LTGDSITAPVVSGQSKGSGAEYLAQIGVGQPVKLFYLVPDTGSDV 186
              +      NE      + +T PVVSG S+GSG EY ++IGVG P K  YLV DTGSDV
Sbjct: 128 VDRSDLKPVYNEDTRYQTEDLTTPVVSGASQGSG-EYFSRIGVGTPAKEMYLVLDTGSDV 187

Query: 187 TWLQCQPCASENTCYKQFDPIFDPKSSSSYSPLSCNSQQCKLLDKANCNSDTCIYQVHYG 246
            W+QC+PCA    CY+Q DP+F+P SSS+Y  L+C++ QC LL+ + C S+ C+YQV YG
Sbjct: 188 NWIQCEPCAD---CYQQSDPVFNPTSSSTYKSLTCSAPQCSLLETSACRSNKCLYQVSYG 247

Query: 247 DGSFTTGELATETLSFGNSNSIPNLPIGCGHDNEGLFAGGAGLIGLGGGAISLSSQLKAS 306
           DGSFT GELAT+T++FGNS  I N+ +GCGHDNEGLF G AGL+GLGGG +S+++Q+KA+
Sbjct: 248 DGSFTVGELATDTVTFGNSGKINNVALGCGHDNEGLFTGAAGLLGLGGGVLSITNQMKAT 307

Query: 307 SFSYCLVNLDSDSSSTLEFNS-NMPSDSLTSPLVKNDRFHSYRYVKVVGISVGGKTLPIS 366
           SFSYCLV+ DS  SS+L+FNS  +     T+PL++N +  ++ YV + G SVGG+ + + 
Sbjct: 308 SFSYCLVDRDSGKSSSLDFNSVQLGGGDATAPLLRNKKIDTFYYVGLSGFSVGGEKVVLP 367

Query: 367 PTRFEIDESGLGGIIVDSGTIISRLPSDVYESLREAFVKLTSSLSP-APGISVFDTCYNF 426
              F++D SG GG+I+D GT ++RL +  Y SLR+AF+KLT +L   +  IS+FDTCY+F
Sbjct: 368 DAIFDVDASGSGGVILDCGTAVTRLQTQAYNSLRDAFLKLTVNLKKGSSSISLFDTCYDF 427

Query: 427 SGQSNVEVPTIAFVLSEGTSLRLPARNYLIMLDTAGTYCLAFIKTKSSLSIIGSFQQQGI 486
           S  S V+VPT+AF  + G SL LPA+NYLI +D +GT+C AF  T SSLSIIG+ QQQG 
Sbjct: 428 SSLSTVKVPTVAFHFTGGKSLDLPAKNYLIPVDDSGTFCFAFAPTSSSLSIIGNVQQQGT 487

Query: 487 RVSYDLTNSLVGFSTNKC 488
           R++YDL+ +++G S NKC
Sbjct: 488 RITYDLSKNVIGLSGNKC 500

[Close]

BLAST of CSPI01G03700 vs. TAIR 10
Match: AT3G20015.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 343.6 bits (880), Expect = 2.6e-94
Identity = 181/435 (41.61%), Postives = 272/435 (62.53%), Query Frame = 0


Query: 60  NHSHL---PNSPFSLPLYPRLALHNPSYKDYNTLVRARLTRDAARVQFLNRNLERSLNGG 119
           N++H     +S ++L L  R    + +Y++++  + AR+ RD  RV  + R +   +   
Sbjct: 47  NNTHFSDESSSKYTLRLLHRDRFPSVTYRNHHHRLHARMRRDTDRVSAILRRISGKVIPS 106

Query: 120 THFGESINESLTGDSITAPVVSGQSKGSGAEYLAQIGVGQPVKLFYLVPDTGSDVTWLQC 179
           +     +N+        + +VSG  +GSG EY  +IGVG P +  Y+V D+GSD+ W+QC
Sbjct: 107 SDSRYEVND------FGSDIVSGMDQGSG-EYFVRIGVGSPPRDQYMVIDSGSDMVWVQC 166

Query: 180 QPCASENTCYKQFDPIFDPKSSSSYSPLSCNSQQCKLLDKANCNSDTCIYQVHYGDGSFT 239
           QPC     CYKQ DP+FDP  S SY+ +SC S  C  ++ + C+S  C Y+V YGDGS+T
Sbjct: 167 QPC---KLCYKQSDPVFDPAKSGSYTGVSCGSSVCDRIENSGCHSGGCRYEVMYGDGSYT 226

Query: 240 TGELATETLSFGNSNSIPNLPIGCGHDNEGLFAGGAGLIGLGGGAISLSSQLK---ASSF 299
            G LA ETL+F  +  + N+ +GCGH N G+F G AGL+G+GGG++S   QL      +F
Sbjct: 227 KGTLALETLTFAKT-VVRNVAMGCGHRNRGMFIGAAGLLGIGGGSMSFVGQLSGQTGGAF 286

Query: 300 SYCLVNLDSDSSSTLEFNSN-MPSDSLTSPLVKNDRFHSYRYVKVVGISVGGKTLPISPT 359
            YCLV+  +DS+ +L F    +P  +   PLV+N R  S+ YV + G+ VGG  +P+   
Sbjct: 287 GYCLVSRGTDSTGSLVFGREALPVGASWVPLVRNPRAPSFYYVGLKGLGVGGVRIPLPDG 346

Query: 360 RFEIDESGLGGIIVDSGTIISRLPSDVYESLREAFVKLTSSLSPAPGISVFDTCYNFSGQ 419
            F++ E+G GG+++D+GT ++RLP+  Y + R+ F   T++L  A G+S+FDTCY+ SG 
Sbjct: 347 VFDLTETGDGGVVMDTGTAVTRLPTAAYVAFRDGFKSQTANLPRASGVSIFDTCYDLSGF 406

Query: 420 SNVEVPTIAFVLSEGTSLRLPARNYLIMLDTAGTYCLAFIKTKSSLSIIGSFQQQGIRVS 479
            +V VPT++F  +EG  L LPARN+L+ +D +GTYC AF  + + LSIIG+ QQ+GI+VS
Sbjct: 407 VSVRVPTVSFYFTEGPVLTLPARNFLMPVDDSGTYCFAFAASPTGLSIIGNIQQEGIQVS 466

Query: 480 YDLTNSLVGFSTNKC 488
           +D  N  VGF  N C
Sbjct: 467 FDGANGFVGFGPNVC 470

[Close]

BLAST of CSPI01G03700 vs. TAIR 10
Match: AT3G61820.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 328.6 bits (841), Expect = 8.8e-90
Identity = 212/504 (42.06%), Postives = 294/504 (58.33%), Query Frame = 0


Query: 1   MNTSLSSVF--LFLTIFTSLQFPSILSRKLTPSSYSTSIFDVSASTNQALDALSIKPKPL 60
           +NT   SVF  LF T   S Q+ +++   L PSS + S  +  + T+   ++LS     L
Sbjct: 6   LNTLAFSVFAVLFFTSSASSQYQTLVVNTL-PSSATLSWPESESLTD---ESLSESTTSL 65

Query: 61  QNH-SHLPNSPFSLPLYPRLALHNPSYKDYNTLVRARLTRDAARVQFLNRNLERSLNGGT 120
             H SH+             AL + S      L   RL RD+ RV+ +      S    T
Sbjct: 66  SVHLSHVD------------ALSSFSDASPADLFNLRLQRDSLRVKSITSLAAVS----T 125

Query: 121 HFGESINESLTGDSITAPVVSGQSKGSGAEYLAQIGVGQPVKLFYLVPDTGSDVTWLQCQ 180
               +     T    +  V+SG S+GSG EY  ++GVG P    Y+V DTGSDV WLQC 
Sbjct: 126 GRNATKRTPRTAGGFSGAVISGLSQGSG-EYFMRLGVGTPATNVYMVLDTGSDVVWLQCS 185

Query: 181 PCASENTCYKQFDPIFDPKSSSSYSPLSCNSQQCKLL-DKANC---NSDTCIYQVHYGDG 240
           PC     CY Q D IFDPK S +++ + C S+ C+ L D + C    S TC+YQV YGDG
Sbjct: 186 PC---KACYNQTDAIFDPKKSKTFATVPCGSRLCRRLDDSSECVTRRSKTCLYQVSYGDG 245

Query: 241 SFTTGELATETLSFGNSNSIPNLPIGCGHDNEGLFAGGAGLIGLGGGAISLSSQLK---A 300
           SFT G+ +TETL+F  +  + ++P+GCGHDNEGLF G AGL+GLG G +S  SQ K    
Sbjct: 246 SFTEGDFSTETLTFHGAR-VDHVPLGCGHDNEGLFVGAAGLLGLGRGGLSFPSQTKNRYN 305

Query: 301 SSFSYCLVNLDSDSS-----STLEF-NSNMPSDSLTSPLVKNDRFHSYRYVKVVGISVGG 360
             FSYCLV+  S  S     ST+ F N+ +P  S+ +PL+ N +  ++ Y++++GISVGG
Sbjct: 306 GKFSYCLVDRTSSGSSSKPPSTIVFGNAAVPKTSVFTPLLTNPKLDTFYYLQLLGISVGG 365

Query: 361 KTLP-ISPTRFEIDESGLGGIIVDSGTIISRLPSDVYESLREAFVKLTSSLSPAPGISVF 420
             +P +S ++F++D +G GG+I+DSGT ++RL    Y +LR+AF    + L  AP  S+F
Sbjct: 366 SRVPGVSESQFKLDATGNGGVIIDSGTSVTRLTQPAYVALRDAFRLGATKLKRAPSYSLF 425

Query: 421 DTCYNFSGQSNVEVPTIAFVLSEGTSLRLPARNYLIMLDTAGTYCLAFIKTKSSLSIIGS 480
           DTC++ SG + V+VPT+ F    G  + LPA NYLI ++T G +C AF  T  SLSIIG+
Sbjct: 426 DTCFDLSGMTTVKVPTVVFHFG-GGEVSLPASNYLIPVNTEGRFCFAFAGTMGSLSIIGN 483

Query: 481 FQQQGIRVSYDLTNSLVGFSTNKC 488
            QQQG RV+YDL  S VGF +  C
Sbjct: 486 IQQQGFRVAYDLVGSRVGFLSRAC 483

[Close]

BLAST of CSPI01G03700 vs. TAIR 10
Match: AT1G01300.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 325.1 bits (832), Expect = 9.7e-89
Identity = 181/413 (43.83%), Postives = 254/413 (61.50%), Query Frame = 0


Query: 83  SYKDYNTLVRARLTRDAARVQFLNRNLERSLNGGTHFGESINESLTGDSITAPVVSGQSK 142
           S K  + L  +RL RD+ RV+ +   L   +      G ++  +      ++ VVSG S+
Sbjct: 84  SNKTPDELFSSRLQRDSRRVKSI-ATLAAQIP-----GRNVTHAPRPGGFSSSVVSGLSQ 143

Query: 143 GSGAEYLAQIGVGQPVKLFYLVPDTGSDVTWLQCQPCASENTCYKQFDPIFDPKSSSSYS 202
           GSG EY  ++GVG P +  Y+V DTGSD+ WLQC PC     CY Q DPIFDP+ S +Y+
Sbjct: 144 GSG-EYFTRLGVGTPARYVYMVLDTGSDIVWLQCAPC---RRCYSQSDPIFDPRKSKTYA 203

Query: 203 PLSCNSQQCKLLDKANCNS--DTCIYQVHYGDGSFTTGELATETLSFGNSNSIPNLPIGC 262
            + C+S  C+ LD A CN+   TC+YQV YGDGSFT G+ +TETL+F   N +  + +GC
Sbjct: 204 TIPCSSPHCRRLDSAGCNTRRKTCLYQVSYGDGSFTVGDFSTETLTF-RRNRVKGVALGC 263

Query: 263 GHDNEGLFAGGAGLIGLGGGAISLSSQLK---ASSFSYCLVNLDSDS--SSTLEFNSNMP 322
           GHDNEGLF G AGL+GLG G +S   Q        FSYCLV+  + S  SS +  N+ + 
Sbjct: 264 GHDNEGLFVGAAGLLGLGKGKLSFPGQTGHRFNQKFSYCLVDRSASSKPSSVVFGNAAVS 323

Query: 323 SDSLTSPLVKNDRFHSYRYVKVVGISVGGKTLP-ISPTRFEIDESGLGGIIVDSGTIISR 382
             +  +PL+ N +  ++ YV ++GISVGG  +P ++ + F++D+ G GG+I+DSGT ++R
Sbjct: 324 RIARFTPLLSNPKLDTFYYVGLLGISVGGTRVPGVTASLFKLDQIGNGGVIIDSGTSVTR 383

Query: 383 LPSDVYESLREAFVKLTSSLSPAPGISVFDTCYNFSGQSNVEVPTIAFVLSEGTSLRLPA 442
           L    Y ++R+AF     +L  AP  S+FDTC++ S  + V+VPT+      G  + LPA
Sbjct: 384 LIRPAYIAMRDAFRVGAKTLKRAPDFSLFDTCFDLSNMNEVKVPTVVLHF-RGADVSLPA 443

Query: 443 RNYLIMLDTAGTYCLAFIKTKSSLSIIGSFQQQGIRVSYDLTNSLVGFSTNKC 488
            NYLI +DT G +C AF  T   LSIIG+ QQQG RV YDL +S VGF+   C
Sbjct: 444 TNYLIPVDTNGKFCFAFAGTMGGLSIIGNIQQQGFRVVYDLASSRVGFAPGGC 484

The following BLAST results are available for this feature:

BLAST of CSPI01G03700 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9LS40	2.8e-117	46.99	Protein ASPARTIC PROTEASE IN GUARD CELL 1 OS=Arabidopsis thaliana OX=3702 GN=ASP...	[more]
Q9LHE3	3.7e-93	41.61	Protein ASPARTIC PROTEASE IN GUARD CELL 2 OS=Arabidopsis thaliana OX=3702 GN=ASP...	[more]
Q9LNJ3	1.4e-87	43.83	Aspartyl protease family protein 2 OS=Arabidopsis thaliana OX=3702 GN=APF2 PE=2 ...	[more]
Q766C3	2.0e-67	39.28	Aspartic proteinase nepenthesin-1 OS=Nepenthes gracilis OX=150966 GN=nep1 PE=1 S...	[more]
Q9LEW3	1.9e-65	38.42	Aspartyl protease AED1 OS=Arabidopsis thaliana OX=3702 GN=AED1 PE=2 SV=1	[more]

BLAST of CSPI01G03700 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0LPJ3	2.9e-274	99.79	Aspartic proteinase nepenthesin-1 OS=Cucumis sativus OX=3659 GN=Csa_1G022490 PE=...	[more]
A0A5A7UQC2	5.9e-251	92.20	Protein ASPARTIC PROTEASE IN GUARD CELL 1-like OS=Cucumis melo var. makuwa OX=11...	[more]
A0A5D3DYG2	1.7e-250	92.20	Protein ASPARTIC PROTEASE IN GUARD CELL 1-like OS=Cucumis melo var. makuwa OX=11...	[more]
A0A1S4E062	9.4e-233	87.27	protein ASPARTIC PROTEASE IN GUARD CELL 1-like OS=Cucumis melo OX=3656 GN=LOC103...	[more]
A0A5A7UQC3	7.3e-201	74.80	Protein ASPARTIC PROTEASE IN GUARD CELL 1 OS=Cucumis melo var. makuwa OX=1194695...	[more]

BLAST of CSPI01G03700 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_004138238.1	6.0e-274	99.79	protein ASPARTIC PROTEASE IN GUARD CELL 1 [Cucumis sativus] >KGN63810.1 hypothet...	[more]
KAA0058063.1	1.2e-250	92.20	protein ASPARTIC PROTEASE IN GUARD CELL 1-like [Cucumis melo var. makuwa]	[more]
TYK28409.1	3.5e-250	92.20	protein ASPARTIC PROTEASE IN GUARD CELL 1-like [Cucumis melo var. makuwa]	[more]
XP_016901370.1	1.9e-232	87.27	PREDICTED: protein ASPARTIC PROTEASE IN GUARD CELL 1-like [Cucumis melo]	[more]
XP_038878113.1	2.2e-223	82.34	protein ASPARTIC PROTEASE IN GUARD CELL 1 [Benincasa hispida]	[more]

BLAST of CSPI01G03700 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G25510.1	4.4e-126	48.45	Eukaryotic aspartyl protease family protein	[more]
AT3G18490.1	2.0e-118	46.99	Eukaryotic aspartyl protease family protein	[more]
AT3G20015.1	2.6e-94	41.61	Eukaryotic aspartyl protease family protein	[more]
AT3G61820.1	8.8e-90	42.06	Eukaryotic aspartyl protease family protein	[more]
AT1G01300.1	9.7e-89	43.83	Eukaryotic aspartyl protease family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Cucumber (PI 183967) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR032861	Xylanase inhibitor, N-terminal	PFAM	PF14543	TAXi_N	coord: 148..310 e-value: 3.5E-47 score: 160.9
IPR021109	Aspartic peptidase domain superfamily	GENE3D	2.40.70.10	Acid Proteases	coord: 121..309 e-value: 1.3E-48 score: 167.5
IPR021109	Aspartic peptidase domain superfamily	GENE3D	2.40.70.10	Acid Proteases	coord: 310..487 e-value: 1.8E-47 score: 163.4
IPR021109	Aspartic peptidase domain superfamily	SUPERFAMILY	50630	Acid proteases	coord: 144..487
IPR032799	Xylanase inhibitor, C-terminal	PFAM	PF14541	TAXi_C	coord: 334..483 e-value: 1.5E-35 score: 122.4
None	No IPR available	PANTHER	PTHR13683:SF274	PROTEIN ASPARTIC PROTEASE IN GUARD CELL 1	coord: 18..487
IPR001461	Aspartic peptidase A1 family	PANTHER	PTHR13683	ASPARTYL PROTEASES	coord: 18..487
IPR033121	Peptidase family A1 domain	PROSITE	PS51767	PEPTIDASE_A1	coord: 148..483 score: 43.276772

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CSPI01G03700.1	CSPI01G03700.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006508	proteolysis
cellular_component	GO:0016021	integral component of membrane
molecular_function	GO:0004190	aspartic-type endopeptidase activity