CSPI01G03070 (gene) Cucumber (PI 183967) v1

Overview


Name	CSPI01G03070
Type	gene
Organism	Cucumis sativus var. hardwickii cv. PI 183967 (Cucumber (PI 183967) v1)
Description	protein MIS12 homolog
Location	Chr1: 1935268 .. 1937214 (+)
RNA-Seq Expression	CSPI01G03070
Synteny	CSPI01G03070

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDSpolypeptidethree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

CATTGGGTAAGAAGGTTTTTCAGGACGCAAAAGGGTAATTTTGATGGAAGGAAGTAAAGGCGAAGTGGTGTTCAATTCACTCAATCTGAATCCACAGCTGTTCATTAATGAAGCTCTCAATACTGTCGACGATTTAGTTGATGATGCTTTCGATTTCTATCAATCGTATTGTTCTCTCTGTTTTCTATTTCTTTAATTTAGGTTTTTCAATTTTGTTCGGTTAGGTTAATTTTGTTGATTCTTGTTAATTCTTTTTCCCTTCAGACAAGCGTCGGCTGCCCTGAAGACCGAGAGCTCGGATAGATCTCAGGATCTTACCCTAGTAAGGTTTAATTTGTATTATGTTATTTAGCATTTTCTTGTTAATTTCTGAATTCTTAAGTTTGTACTCTTAAGCACTTCTTTGAATTGTTCACATTTTCGCCTCGTTTTATTCAGGGAATATCGCAAGTCCGAGCGTTGGTCCAATTGGGTCTGCATAAGCGATTGGCCATGTGGGAAAAGTACTGCCTTAATCACTGTTTTTCAGTGCCTGAAGGTTTTTCTTTACCCTCAAATGTAGGTTCTTCTTTCTTAATGAGGCTCCTTGGCCAAAGAATAAAATATCATGTTGGTATCAATATCTGAATTTTAGTTTGTAGTTTATCGATTATATATCAACCTTGATAGATATTTATGTAAAGCAATAAATTAACCGAAATTAGCTATTGAATTGCTTTTGCTTTCAAACTAAGTTGTAGATCTTGCTTTTAAGTAATATTCATATTAAGATTAATAGATGACATCTTTTTATGTTTTATGTGTTTATAAAGGATAGTTGAGATGTTTATAAACCCTTAATATTGATGTCAAATATTCATATATATATATATAACTTTGCCGGTTTCAAAAGTTCTCTATAAATATAACTTACATGTAAATGACCGGTTGCTTATGCAGCGAGTTTCCCATTTATTTTGTACTGACTTGATAGCTTCCTCAATTTTTGGTTCTGGGACGAACAAATTTTGAGGAGAAAGCGCAGTTACTTACTGTGTATCATTGAGTTTCAATTTTTGTTCCAAATATATTCATTGCTACTTTCAGATATAATCACAAGTTTCCATATTTTCAGACCTCAGAGCTTCCTCATTTTGTTAGCAATAACTCAACGTGGTTCCATTGTAACTAGAATGTGGTCGTGACGGTAAAAGATTCTTTTTAGCTCTAAAAGGAATGCGACCAAATGCCTGCAGCACTTATAAAAATCTGTCACTGGGGCATCATGCCTTTTCTTTTTCAATTACGTTTGAAATCTCCTATGTAAGATCCAGTTCGATTCCTTTCATTTTGCACCAATATAGTTTACTTTGGGTTGGGCAACTTCTGTCTGTTTCACTGGCATCTCATTGGAATCCCCAATATCTTATTGTCAGGATGCTAAATTATCAATGGTTCCCATCTTGTTTGAGAACTTTGAGTTATGGCTAATTGAGTCTATGTGTTCTTTTAATCGAGTCTGTGTTCTTTTACTACTTTTTCTTCTTTCTAATGCAATAGCTAATAAACATATTTCTGATTCAGATCTAAATATTTTCTTTTAAGTTGTCTTAGGATGAATCACCTGGTGTCACTTCAATAAGTCATGACCATGACGTGGATCTGGATACAGAGCTTGATCTTTTGAGAAATAAGCTCTCCGAGGTATTGAATATGTCGTATGTTTAACACGTGCGTTTAAGTAATTCATATCTCATGAGGAAATATATTCTTCAGGTCAGAAAAGAGAATATTGTATTAAACCAAGAACTGCAAGCTTTGGAAAGGCAAACTGCTTCCAGTAACTCCCAAATCAGTCATTTCAACGAAGCATTACAACTATATGAGCAAAGTTCTGTGAATGACATGTTCCAGGGTAACTAAGTTGTGCTTAAAACCAATTTTTTCTTCCTGCACTCCTGGGTTTAC

mRNA sequence

CATTGGGTAAGAAGGTTTTTCAGGACGCAAAAGGGTAATTTTGATGGAAGGAAGTAAAGGCGAAGTGGTGTTCAATTCACTCAATCTGAATCCACAGCTGTTCATTAATGAAGCTCTCAATACTGTCGACGATTTAGTTGATGATGCTTTCGATTTCTATCAATCACAAGCGTCGGCTGCCCTGAAGACCGAGAGCTCGGATAGATCTCAGGATCTTACCCTAGGAATATCGCAAGTCCGAGCGTTGGTCCAATTGGGTCTGCATAAGCGATTGGCCATGTGGGAAAAGTACTGCCTTAATCACTGTTTTTCAGTGCCTGAAGGTTTTTCTTTACCCTCAAATGATGAATCACCTGGTGTCACTTCAATAAGTCATGACCATGACGTGGATCTGGATACAGAGCTTGATCTTTTGAGAAATAAGCTCTCCGAGGTCAGAAAAGAGAATATTGTATTAAACCAAGAACTGCAAGCTTTGGAAAGGCAAACTGCTTCCAGTAACTCCCAAATCAGTCATTTCAACGAAGCATTACAACTATATGAGCAAAGTTCTGTGAATGACATGTTCCAGGGTAACTAAGTTGTGCTTAAAACCAATTTTTTCTTCCTGCACTCCTGGGTTTAC

Coding sequence (CDS)

ATGGAAGGAAGTAAAGGCGAAGTGGTGTTCAATTCACTCAATCTGAATCCACAGCTGTTCATTAATGAAGCTCTCAATACTGTCGACGATTTAGTTGATGATGCTTTCGATTTCTATCAATCACAAGCGTCGGCTGCCCTGAAGACCGAGAGCTCGGATAGATCTCAGGATCTTACCCTAGGAATATCGCAAGTCCGAGCGTTGGTCCAATTGGGTCTGCATAAGCGATTGGCCATGTGGGAAAAGTACTGCCTTAATCACTGTTTTTCAGTGCCTGAAGGTTTTTCTTTACCCTCAAATGATGAATCACCTGGTGTCACTTCAATAAGTCATGACCATGACGTGGATCTGGATACAGAGCTTGATCTTTTGAGAAATAAGCTCTCCGAGGTCAGAAAAGAGAATATTGTATTAAACCAAGAACTGCAAGCTTTGGAAAGGCAAACTGCTTCCAGTAACTCCCAAATCAGTCATTTCAACGAAGCATTACAACTATATGAGCAAAGTTCTGTGAATGACATGTTCCAGGGTAACTAA

Protein sequence

MEGSKGEVVFNSLNLNPQLFINEALNTVDDLVDDAFDFYQSQASAALKTESSDRSQDLTLGISQVRALVQLGLHKRLAMWEKYCLNHCFSVPEGFSLPSNDESPGVTSISHDHDVDLDTELDLLRNKLSEVRKENIVLNQELQALERQTASSNSQISHFNEALQLYEQSSVNDMFQGN*

Homology

[Close]

BLAST of CSPI01G03070 vs. ExPASy Swiss-Prot
Match: Q2V0Z5 (Protein MIS12 homolog OS=Arabidopsis thaliana OX=3702 GN=MIS12 PE=3 SV=1)

HSP 1 Score: 168.3 bits (425), Expect = 7.8e-41
Identity = 91/180 (50.56%), Postives = 130/180 (72.22%), Query Frame = 0


Query: 1   MEGSKGEVVFNSLNLNPQLFINEALNTVDDLVDDAFDFYQSQASAALKTESSD--RSQDL 60
           MEGSK E VF+S+NLNPQ+FINEA+N+V+D VD AFDFY   AS +LK + SD  +SQ L
Sbjct: 1   MEGSKSEAVFDSMNLNPQIFINEAINSVEDYVDQAFDFYARDASKSLKIKGSDKQKSQAL 60

Query: 61  TLGISQVRALVQLGLHKRLAMWEKYCLNHCFSVPEGFSLPSNDESPGVTSISHD--HDVD 120
           + GI++VR L+   +  RL +WE Y L  CF+VP+GF LP ++ES   +S+  D  +D++
Sbjct: 61  SNGIARVRGLLLSVIDNRLKLWESYSLRFCFAVPDGFVLPKSEES---SSVHQDGLYDLE 120

Query: 121 LDTELDLLRNKLSEVRKENIVLNQELQALERQTASSNSQISHFNEALQLYEQSSVNDMFQ 177
           LD ELD LR+KL+ V K ++ L+ ELQALER + S    +   NEAL+LY++SS++++F+
Sbjct: 121 LDAELDSLRDKLNVVGKRSVELDSELQALERSSVSRERSLRLVNEALELYDESSMDEIFK 177

[Close]

BLAST of CSPI01G03070 vs. ExPASy TrEMBL
Match: A0A0A0LPL2 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G014450 PE=3 SV=1)

HSP 1 Score: 340.1 bits (871), Expect = 5.5e-90
Identity = 175/176 (99.43%), Postives = 176/176 (100.00%), Query Frame = 0


Query: 1   MEGSKGEVVFNSLNLNPQLFINEALNTVDDLVDDAFDFYQSQASAALKTESSDRSQDLTL 60
           MEGSKGEVVFNSLNLNPQLFINEALNTVDDLVDDAFDFYQSQASAALKTESSDRSQDLTL
Sbjct: 1   MEGSKGEVVFNSLNLNPQLFINEALNTVDDLVDDAFDFYQSQASAALKTESSDRSQDLTL 60

Query: 61  GISQVRALVQLGLHKRLAMWEKYCLNHCFSVPEGFSLPSNDESPGVTSISHDHDVDLDTE 120
           GISQVRALVQLGLHKRLAMWEKYCLNHCFSVPEGFSLPS+DESPGVTSISHDHDVDLDTE
Sbjct: 61  GISQVRALVQLGLHKRLAMWEKYCLNHCFSVPEGFSLPSDDESPGVTSISHDHDVDLDTE 120

Query: 121 LDLLRNKLSEVRKENIVLNQELQALERQTASSNSQISHFNEALQLYEQSSVNDMFQ 177
           LDLLRNKLSEVRKENIVLNQELQALERQTASSNSQISHFNEALQLYEQSSVNDMFQ
Sbjct: 121 LDLLRNKLSEVRKENIVLNQELQALERQTASSNSQISHFNEALQLYEQSSVNDMFQ 176

[Close]

BLAST of CSPI01G03070 vs. ExPASy TrEMBL
Match: A0A5A7US57 (Centromere protein Mis12 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold629G00040 PE=3 SV=1)

HSP 1 Score: 330.5 bits (846), Expect = 4.4e-87
Identity = 171/176 (97.16%), Postives = 172/176 (97.73%), Query Frame = 0


Query: 1   MEGSKGEVVFNSLNLNPQLFINEALNTVDDLVDDAFDFYQSQASAALKTESSDRSQDLTL 60
           MEGSKGE VFNSLNLNPQLFINEALNTVDDLVDDAFDFYQSQASAALKTE SDRSQDLTL
Sbjct: 1   MEGSKGESVFNSLNLNPQLFINEALNTVDDLVDDAFDFYQSQASAALKTEGSDRSQDLTL 60

Query: 61  GISQVRALVQLGLHKRLAMWEKYCLNHCFSVPEGFSLPSNDESPGVTSISHDHDVDLDTE 120
           GISQVRALVQLGL KRLAMWEKYCLNHCFSVPEGFSLPSNDESPGVTSI+HD DVDLDTE
Sbjct: 61  GISQVRALVQLGLDKRLAMWEKYCLNHCFSVPEGFSLPSNDESPGVTSINHDQDVDLDTE 120

Query: 121 LDLLRNKLSEVRKENIVLNQELQALERQTASSNSQISHFNEALQLYEQSSVNDMFQ 177
           LDLLRNKLSEVRKENIVLNQELQALERQTASSNSQISHFNEALQLYEQSSVNDMFQ
Sbjct: 121 LDLLRNKLSEVRKENIVLNQELQALERQTASSNSQISHFNEALQLYEQSSVNDMFQ 176

[Close]

BLAST of CSPI01G03070 vs. ExPASy TrEMBL
Match: A0A5A7UQ95 (Centromere protein Mis12 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold274G003370 PE=3 SV=1)

HSP 1 Score: 328.9 bits (842), Expect = 1.3e-86
Identity = 170/176 (96.59%), Postives = 171/176 (97.16%), Query Frame = 0


Query: 1   MEGSKGEVVFNSLNLNPQLFINEALNTVDDLVDDAFDFYQSQASAALKTESSDRSQDLTL 60
           MEGSKGE VFNSLNLNPQLFINEALNTVDDLVDDAFDFYQSQASA LKTE SDRSQDLTL
Sbjct: 1   MEGSKGESVFNSLNLNPQLFINEALNTVDDLVDDAFDFYQSQASATLKTEGSDRSQDLTL 60

Query: 61  GISQVRALVQLGLHKRLAMWEKYCLNHCFSVPEGFSLPSNDESPGVTSISHDHDVDLDTE 120
           GISQVRALVQLGL KRLAMWEKYCLNHCFSVPEGFSLPSNDESPGVTSI+HD DVDLDTE
Sbjct: 61  GISQVRALVQLGLDKRLAMWEKYCLNHCFSVPEGFSLPSNDESPGVTSINHDQDVDLDTE 120

Query: 121 LDLLRNKLSEVRKENIVLNQELQALERQTASSNSQISHFNEALQLYEQSSVNDMFQ 177
           LDLLRNKLSEVRKENIVLNQELQALERQTASSNSQISHFNEALQLYEQSSVNDMFQ
Sbjct: 121 LDLLRNKLSEVRKENIVLNQELQALERQTASSNSQISHFNEALQLYEQSSVNDMFQ 176

[Close]

BLAST of CSPI01G03070 vs. ExPASy TrEMBL
Match: A0A6J1E0A1 (protein MIS12 homolog OS=Cucurbita moschata OX=3662 GN=LOC111429660 PE=3 SV=1)

HSP 1 Score: 292.4 bits (747), Expect = 1.3e-75
Identity = 152/178 (85.39%), Postives = 163/178 (91.57%), Query Frame = 0


Query: 1   MEGSKGEVVFNSLNLNPQLFINEALNTVDDLVDDAFDFYQSQASAALKTESSDRSQDLTL 60
           MEGSKGE VF+SLNLNPQLFINEALN VDDLVDDAFDFYQ+QASA+LKTE SDRSQDL L
Sbjct: 1   MEGSKGEEVFDSLNLNPQLFINEALNIVDDLVDDAFDFYQTQASASLKTEGSDRSQDLIL 60

Query: 61  GISQVRALVQLGLHKRLAMWEKYCLNHCFSVPEGFSLPSNDESPGVTSISHD--HDVDLD 120
           GIS+VR  VQ GL KRLAMWEKYCLNHCFSVPEGFSLPSNDESP VTS+S D  +DVDLD
Sbjct: 61  GISRVRTFVQSGLDKRLAMWEKYCLNHCFSVPEGFSLPSNDESPVVTSVSRDCLYDVDLD 120

Query: 121 TELDLLRNKLSEVRKENIVLNQELQALERQTASSNSQISHFNEALQLYEQSSVNDMFQ 177
           TELD LRNKLSEVRKENIVLNQELQALERQ ASSNSQ++ FNEALQLYE++SVN+MFQ
Sbjct: 121 TELDFLRNKLSEVRKENIVLNQELQALERQAASSNSQVNRFNEALQLYERNSVNEMFQ 178

[Close]

BLAST of CSPI01G03070 vs. ExPASy TrEMBL
Match: A0A6J1JD43 (protein MIS12 homolog isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111485708 PE=3 SV=1)

HSP 1 Score: 291.6 bits (745), Expect = 2.3e-75
Identity = 152/178 (85.39%), Postives = 162/178 (91.01%), Query Frame = 0


Query: 1   MEGSKGEVVFNSLNLNPQLFINEALNTVDDLVDDAFDFYQSQASAALKTESSDRSQDLTL 60
           MEGSKGE VF+SLNLNPQLFINEALN VDDLVDDAFDFYQ+QASA+LKTE SDRSQDL L
Sbjct: 1   MEGSKGEEVFDSLNLNPQLFINEALNIVDDLVDDAFDFYQTQASASLKTEGSDRSQDLIL 60

Query: 61  GISQVRALVQLGLHKRLAMWEKYCLNHCFSVPEGFSLPSNDESPGVTSISHD--HDVDLD 120
           GIS+VR  VQ GL KRLAMWEKYCLNHCFSVPEGFSLPSNDESP VTS+S D  +DVDLD
Sbjct: 61  GISRVRTFVQSGLDKRLAMWEKYCLNHCFSVPEGFSLPSNDESPVVTSVSRDCLYDVDLD 120

Query: 121 TELDLLRNKLSEVRKENIVLNQELQALERQTASSNSQISHFNEALQLYEQSSVNDMFQ 177
           TELD LR  LSEVRKENIVLNQELQALERQTASSNSQ++ FNEALQLYEQ+SVN+MFQ
Sbjct: 121 TELDFLRKNLSEVRKENIVLNQELQALERQTASSNSQVNRFNEALQLYEQNSVNEMFQ 178

[Close]

BLAST of CSPI01G03070 vs. NCBI nr
Match: XP_004138204.3 (protein MIS12 homolog isoform X1 [Cucumis sativus])

HSP 1 Score: 340.1 bits (871), Expect = 1.1e-89
Identity = 175/176 (99.43%), Postives = 176/176 (100.00%), Query Frame = 0


Query: 1   MEGSKGEVVFNSLNLNPQLFINEALNTVDDLVDDAFDFYQSQASAALKTESSDRSQDLTL 60
           MEGSKGEVVFNSLNLNPQLFINEALNTVDDLVDDAFDFYQSQASAALKTESSDRSQDLTL
Sbjct: 1   MEGSKGEVVFNSLNLNPQLFINEALNTVDDLVDDAFDFYQSQASAALKTESSDRSQDLTL 60

Query: 61  GISQVRALVQLGLHKRLAMWEKYCLNHCFSVPEGFSLPSNDESPGVTSISHDHDVDLDTE 120
           GISQVRALVQLGLHKRLAMWEKYCLNHCFSVPEGFSLPS+DESPGVTSISHDHDVDLDTE
Sbjct: 61  GISQVRALVQLGLHKRLAMWEKYCLNHCFSVPEGFSLPSDDESPGVTSISHDHDVDLDTE 120

Query: 121 LDLLRNKLSEVRKENIVLNQELQALERQTASSNSQISHFNEALQLYEQSSVNDMFQ 177
           LDLLRNKLSEVRKENIVLNQELQALERQTASSNSQISHFNEALQLYEQSSVNDMFQ
Sbjct: 121 LDLLRNKLSEVRKENIVLNQELQALERQTASSNSQISHFNEALQLYEQSSVNDMFQ 176

[Close]

BLAST of CSPI01G03070 vs. NCBI nr
Match: KAA0058004.1 (Centromere protein Mis12 [Cucumis melo var. makuwa] >TYK28378.1 Centromere protein Mis12 [Cucumis melo var. makuwa])

HSP 1 Score: 330.5 bits (846), Expect = 9.1e-87
Identity = 171/176 (97.16%), Postives = 172/176 (97.73%), Query Frame = 0


Query: 1   MEGSKGEVVFNSLNLNPQLFINEALNTVDDLVDDAFDFYQSQASAALKTESSDRSQDLTL 60
           MEGSKGE VFNSLNLNPQLFINEALNTVDDLVDDAFDFYQSQASAALKTE SDRSQDLTL
Sbjct: 1   MEGSKGESVFNSLNLNPQLFINEALNTVDDLVDDAFDFYQSQASAALKTEGSDRSQDLTL 60

Query: 61  GISQVRALVQLGLHKRLAMWEKYCLNHCFSVPEGFSLPSNDESPGVTSISHDHDVDLDTE 120
           GISQVRALVQLGL KRLAMWEKYCLNHCFSVPEGFSLPSNDESPGVTSI+HD DVDLDTE
Sbjct: 61  GISQVRALVQLGLDKRLAMWEKYCLNHCFSVPEGFSLPSNDESPGVTSINHDQDVDLDTE 120

Query: 121 LDLLRNKLSEVRKENIVLNQELQALERQTASSNSQISHFNEALQLYEQSSVNDMFQ 177
           LDLLRNKLSEVRKENIVLNQELQALERQTASSNSQISHFNEALQLYEQSSVNDMFQ
Sbjct: 121 LDLLRNKLSEVRKENIVLNQELQALERQTASSNSQISHFNEALQLYEQSSVNDMFQ 176

[Close]

BLAST of CSPI01G03070 vs. NCBI nr
Match: KAA0058032.1 (Centromere protein Mis12 [Cucumis melo var. makuwa])

HSP 1 Score: 328.9 bits (842), Expect = 2.6e-86
Identity = 170/176 (96.59%), Postives = 171/176 (97.16%), Query Frame = 0


Query: 1   MEGSKGEVVFNSLNLNPQLFINEALNTVDDLVDDAFDFYQSQASAALKTESSDRSQDLTL 60
           MEGSKGE VFNSLNLNPQLFINEALNTVDDLVDDAFDFYQSQASA LKTE SDRSQDLTL
Sbjct: 1   MEGSKGESVFNSLNLNPQLFINEALNTVDDLVDDAFDFYQSQASATLKTEGSDRSQDLTL 60

Query: 61  GISQVRALVQLGLHKRLAMWEKYCLNHCFSVPEGFSLPSNDESPGVTSISHDHDVDLDTE 120
           GISQVRALVQLGL KRLAMWEKYCLNHCFSVPEGFSLPSNDESPGVTSI+HD DVDLDTE
Sbjct: 61  GISQVRALVQLGLDKRLAMWEKYCLNHCFSVPEGFSLPSNDESPGVTSINHDQDVDLDTE 120

Query: 121 LDLLRNKLSEVRKENIVLNQELQALERQTASSNSQISHFNEALQLYEQSSVNDMFQ 177
           LDLLRNKLSEVRKENIVLNQELQALERQTASSNSQISHFNEALQLYEQSSVNDMFQ
Sbjct: 121 LDLLRNKLSEVRKENIVLNQELQALERQTASSNSQISHFNEALQLYEQSSVNDMFQ 176

[Close]

BLAST of CSPI01G03070 vs. NCBI nr
Match: XP_038879945.1 (protein MIS12 homolog [Benincasa hispida])

HSP 1 Score: 312.8 bits (800), Expect = 2.0e-81
Identity = 160/176 (90.91%), Postives = 168/176 (95.45%), Query Frame = 0


Query: 1   MEGSKGEVVFNSLNLNPQLFINEALNTVDDLVDDAFDFYQSQASAALKTESSDRSQDLTL 60
           MEGSKGE VF+SLNLNPQLFINEALN VDDLVDDAF FYQSQASA LKTE SDRSQDLTL
Sbjct: 31  MEGSKGEAVFDSLNLNPQLFINEALNIVDDLVDDAFGFYQSQASATLKTEGSDRSQDLTL 90

Query: 61  GISQVRALVQLGLHKRLAMWEKYCLNHCFSVPEGFSLPSNDESPGVTSISHDHDVDLDTE 120
           GIS+VRALVQLGL KRLAMWEKYCLNHCFSVPEGFSLPSND+SPGVTSISHD+DV LDTE
Sbjct: 91  GISRVRALVQLGLDKRLAMWEKYCLNHCFSVPEGFSLPSNDDSPGVTSISHDYDVGLDTE 150

Query: 121 LDLLRNKLSEVRKENIVLNQELQALERQTASSNSQISHFNEALQLYEQSSVNDMFQ 177
           LD LRNKLSEVRKENIVLNQELQALERQTA+SNSQI+HFNEALQLYEQ+SVN+MFQ
Sbjct: 151 LDFLRNKLSEVRKENIVLNQELQALERQTATSNSQINHFNEALQLYEQNSVNEMFQ 206

[Close]

BLAST of CSPI01G03070 vs. NCBI nr
Match: XP_023515499.1 (protein MIS12 homolog isoform X2 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 296.2 bits (757), Expect = 1.9e-76
Identity = 153/178 (85.96%), Postives = 165/178 (92.70%), Query Frame = 0


Query: 1   MEGSKGEVVFNSLNLNPQLFINEALNTVDDLVDDAFDFYQSQASAALKTESSDRSQDLTL 60
           MEGSKGE VF+SLNLNPQLFINEALN VDDLVDDAFDFYQ+QASA+LKTE SDRSQDL+L
Sbjct: 1   MEGSKGEEVFDSLNLNPQLFINEALNIVDDLVDDAFDFYQTQASASLKTEGSDRSQDLSL 60

Query: 61  GISQVRALVQLGLHKRLAMWEKYCLNHCFSVPEGFSLPSNDESPGVTSISHD--HDVDLD 120
           GIS+VR  VQ GL KRLAMWEKYCLNHCFSVPEGFSLPSNDESP VTS+S D  +DVDLD
Sbjct: 61  GISRVRTFVQSGLDKRLAMWEKYCLNHCFSVPEGFSLPSNDESPVVTSVSRDCLYDVDLD 120

Query: 121 TELDLLRNKLSEVRKENIVLNQELQALERQTASSNSQISHFNEALQLYEQSSVNDMFQ 177
           TELD LRNKLSEVRKEN+VLNQELQALERQTASSNSQ++ FNEALQLYEQ+SVN+MFQ
Sbjct: 121 TELDFLRNKLSEVRKENVVLNQELQALERQTASSNSQVNRFNEALQLYEQNSVNEMFQ 178

[Close]

BLAST of CSPI01G03070 vs. TAIR 10
Match: AT5G35520.1 (minichromosome instability 12 (mis12)-like )

HSP 1 Score: 168.3 bits (425), Expect = 5.6e-42
Identity = 91/180 (50.56%), Postives = 130/180 (72.22%), Query Frame = 0


Query: 1   MEGSKGEVVFNSLNLNPQLFINEALNTVDDLVDDAFDFYQSQASAALKTESSD--RSQDL 60
           MEGSK E VF+S+NLNPQ+FINEA+N+V+D VD AFDFY   AS +LK + SD  +SQ L
Sbjct: 1   MEGSKSEAVFDSMNLNPQIFINEAINSVEDYVDQAFDFYARDASKSLKIKGSDKQKSQAL 60

Query: 61  TLGISQVRALVQLGLHKRLAMWEKYCLNHCFSVPEGFSLPSNDESPGVTSISHD--HDVD 120
           + GI++VR L+   +  RL +WE Y L  CF+VP+GF LP ++ES   +S+  D  +D++
Sbjct: 61  SNGIARVRGLLLSVIDNRLKLWESYSLRFCFAVPDGFVLPKSEES---SSVHQDGLYDLE 120

Query: 121 LDTELDLLRNKLSEVRKENIVLNQELQALERQTASSNSQISHFNEALQLYEQSSVNDMFQ 177
           LD ELD LR+KL+ V K ++ L+ ELQALER + S    +   NEAL+LY++SS++++F+
Sbjct: 121 LDAELDSLRDKLNVVGKRSVELDSELQALERSSVSRERSLRLVNEALELYDESSMDEIFK 177

The following BLAST results are available for this feature:

BLAST of CSPI01G03070 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q2V0Z5	7.8e-41	50.56	Protein MIS12 homolog OS=Arabidopsis thaliana OX=3702 GN=MIS12 PE=3 SV=1	[more]

BLAST of CSPI01G03070 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0LPL2	5.5e-90	99.43	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G014450 PE=3 SV=1	[more]
A0A5A7US57	4.4e-87	97.16	Centromere protein Mis12 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffol...	[more]
A0A5A7UQ95	1.3e-86	96.59	Centromere protein Mis12 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffol...	[more]
A0A6J1E0A1	1.3e-75	85.39	protein MIS12 homolog OS=Cucurbita moschata OX=3662 GN=LOC111429660 PE=3 SV=1	[more]
A0A6J1JD43	2.3e-75	85.39	protein MIS12 homolog isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111485708 PE=...	[more]

BLAST of CSPI01G03070 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_004138204.3	1.1e-89	99.43	protein MIS12 homolog isoform X1 [Cucumis sativus]	[more]
KAA0058004.1	9.1e-87	97.16	Centromere protein Mis12 [Cucumis melo var. makuwa] >TYK28378.1 Centromere prote...	[more]
KAA0058032.1	2.6e-86	96.59	Centromere protein Mis12 [Cucumis melo var. makuwa]	[more]
XP_038879945.1	2.0e-81	90.91	protein MIS12 homolog [Benincasa hispida]	[more]
XP_023515499.1	1.9e-76	85.96	protein MIS12 homolog isoform X2 [Cucurbita pepo subsp. pepo]	[more]

BLAST of CSPI01G03070 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G35520.1	5.6e-42	50.56	minichromosome instability 12 (mis12)-like	[more]

InterPro

Analysis Name: InterPro Annotations of Cucumber (PI 183967) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	COILS	Coil	Coil	coord: 121..148
IPR008685	Centromere protein Mis12	PFAM	PF05859	Mis12	coord: 10..139 e-value: 1.9E-19 score: 70.1
IPR008685	Centromere protein Mis12	PANTHER	PTHR14527	PROTEIN MIS12 HOMOLOG	coord: 1..176

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CSPI01G03070.1	CSPI01G03070.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0051301	cell division
biological_process	GO:0051382	kinetochore assembly
biological_process	GO:0000070	mitotic sister chromatid segregation
biological_process	GO:0034501	protein localization to kinetochore
biological_process	GO:0000278	mitotic cell cycle
cellular_component	GO:0000818	nuclear MIS12/MIND complex
cellular_component	GO:0000775	chromosome, centromeric region
cellular_component	GO:0005634	nucleus