Bhi12G000009 (gene) Wax gourd (B227) v1

Overview


Name	Bhi12G000009
Type	gene
Organism	Benincasa hispida (Wax gourd (B227) v1)
Description	Pectinesterase
Location	chr12: 343318 .. 346717 (+)
RNA-Seq Expression	Bhi12G000009
Synteny	Bhi12G000009

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRexonCDSpolypeptidethree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

AATTATTTCCCCACCAATACAAATAGCCCAAAACCCATCAACCGAAAACACTCAAAAAAATTAGAAACAAAAAAAGATGGCTCAAAAGCTTCATTTTTCCATCATTCTAATTTCTTTGTTCATCCTCTCATCAATTTCCCTATGTTTCTCCACCAAACCTAACAACAATGCCATAGATTTATGGTGCAGCCGAATGCCATATCCCGACGTATGCAAACACTTCTTCAATGGCCAATTCGACCCAAAAAAGCTTTCAGAATTCAAAAAAACAGCTCTTAAAATCGCCATGGATAGAGCTATAAGAGCTGAAACACTCACAAAAGAGTTAGGCAAAAAATGCCGTAACGAAAAAGAAAGAGCTGCATGGGCTGACTGTTTGGAGCTTTACCAAACCACAATCCTCCACCTCAACAAAACCTTCTCCGACGAAAAATGCTCTGACTTCGACATCCAAACATGGCTCAGCTCTGCCTTGACCGACCTCGAAACGTGCCGTGCCGGGTTCATCGACCTTAAGATAAAAGATTACGATGTGGTTTTCCCGTTTTTGGCGAACAATAATGTGACAAAGCTTATTAGTAATAGTTTGGCAATGAATAACTGCTCCGAGAGTGATGTGGGGTATAAGAGTAATGAAGGGTTTCCGAAGTGGGTTCGGCGTGGGGACCGGCGGCTGTTGCAGGCGGCGGAGCCTAAGGCGGATTTGGTGGTGGCACAGGACGGGTCGGGGAATTATAGGACGGTGCAAGCGGCGGTGGATGCGGCGGGGAAGAGGAAAGGGAGTGGGAGATTTGTGATACATATAAAGAGGGGAGTTTATAAAGAGAATGTAGTGATAAAGGTGAAGAATTTGATGCTTGTTGGAGATGGATTGAGGTATACAATTATTACTGGAAGTAGAAGTGTTGGTGGTGGTTCTACTACTTTCAACTCTGCTACTGTTGGTGAGTTTTTCTCTTTTTTTTTGTTTTTTAACCGAAAATTTTTGTGAATAGTTTGATTTAGTCTCCCAATATAAGAAAATAAATTAAAATATTTATAAATATAACAAAATTTTATTGATAGACCGCTATAGATTGCTATTCATATCTTCTGTATTATGATAGACACAGATAGTAATCTATTCCGATCTATTGCAGATAGATTATGATATTTTGCTTTAAATATTTTCTAACAGTTTTATCATTTAAAATAATTTTTTTAAAGAATTTTGTATTATCTATTAATATTTTCATAATAAATGCTGAAACAATATTCATGGATATTTTCTTGCATATTATTATTATTATTATTTAATCAATTTAGATTAATAATTAAAGAGACTAAATTTAAGATTTATCGAAAGTATTAAAATTGGATATTTAAAAATATAGAGAGTAAAATAGAACAAATCTTGAACTACAAAAACTAAAATAAACTTGCATTTTCTCTATCTGTACTTTGGATTTCATTATATTTTGGTCCTTCTACTTTTGATATTCAATAAATTTAATCCTTGTATTTTTTTTTATGAATCTTAACATATTCCTGATTAGTTTATTTGTTGATTCTTTTTTGCTGAAAAAAAAAAAAAAAAAACCTATTAGCATCATCTATCAATTTCGAAAATATATTCCTATAGTATTTTTATAACATGAAAATTATTGTTATTCTTTAACCAAAATTAAAAAACCATCTTTTGAAAGATTAAATTTGAGATTTGTTTAAAATGTAAGGACTAAAATTAGACCATTAAAATTGCAGATACTAATGATCAAGGACTAAAATTAGACCATTAAAATTGCAGATACTAATGATCCCTTTGGTAACCATTTAGATTTGGATTTTTATTTTTAAAAAATTAAGATTATAAGTATTATTCCTATCCCAAATTTCTTGGTTAGTTATCTACTTTTTAGCAATAGTTTAAAAAACCAAGCCAAAATTTGAAAACTAACAAAAAAAACTTTTTAAAAAAATGTTTTTGTTTTTTATATTTAGCTAAGAATTCAATCACTGTATTTGATAAGATATAAATCATCCTAATAAATGAAGAAAAAATAGGTTTAATTTTAAAAAATAAAAATAAAAAATAAAACGATTACCAAACAAGACTTGAAATGTGAAATTCAAAGGTACAATAAGACCAATATATATATATATATATATTAACATATAATTTATTGGATTTGTATTTTATTTTGCCTCAACTTTCTACATTATTTGATTTTTGTTCGTAAATTTGTATGTCTAATTACTTAATATTGATTCAAAATTTACCCTAGTCTTTATTAGATGAGTTTGGTAATTATCATAGAAAAAAAATCTCATGTGAGTAGCTTAGAAACAAAAGGAGTCTTAGTTCAAAATTCTTGAACAGTGTTAATGTTACAAAAAAGAGTTTACCATTTCACATCCCTCTTCTCATTTTAGCAAATTAAATCCTTTGTTAAAAAAATGTCTAATATAACATTTTGATATATTTTGTGACTCTCTTCAGCTGTGACCGGTGAACGTTTCATTGCCCGTGGCATCACATTCCGCAACACCGCCGGACCTGAAAACCACCAAGCGGTAGCCCTTCGGTCAGGCTCCGATCTCTCAGTCTTCTTCCGGTGCGGTTTCGAAGGATACCAAGACACTCTCTACGCCCATTCACAAAGACAATTCTATAGAGAATGCTACATTTACGGCACGGTGGATTTCATCTTCGGCAACGCCGCCGTAGTTCTCCAGAACTGCATGATCTACGCAAGAAAACCAATCCCAGGGCAGCAATGCGTCGTCACCGCCCAAGGCCGAACCGACCCAAACCAAAACACAGGGATCTCAATCCACAATTCGAGAGTGATGGCGGCGGACGACCTAAAACCGGTCGTAGGGTCGGTTAGAACCTACTTGGGGAGGCCATGGAAGGAGTACTCTCGCACAGTTTACTTACAGAACTTCATGGATAACTTGGTGAATCCAGCAGGGTGGTTGGAATGGAGTGGTAATTTTGCATTGAATACATTGTATTATGGAGAGTTTAAGAATTTTGGGCCAGGTTCTTCTACTGCTAAGAGAGTGAAATGGAAGGGTTATAGAGTGATAACAAGTGCTACAGAAGCATCAAAATTTACTGTTGGGAATTTCATTGCTGGGAGATCATGGCTGCCTGGAACTGGAGTGCCATTCACTGCTGGGCTTTGAAATAATTATAGTGCATAAAAGTACATGAAGTTTGTGGTTAATTTAATTATTGGGTGATTGGAAGAGAGCATCTTGATTCATGTATCATTTGTTGGGGATTTCAATTTTATTATAGTACTTGTATCAATTATGTACCAATTCTTGGGGAAAGTTTATCTATGATTCTTTGTTGTTTCTTTCAGCCTAATTTGGATCTAATATATTTTTCTTGAGGGAATGAGTTTTTTTA

mRNA sequence

AATTATTTCCCCACCAATACAAATAGCCCAAAACCCATCAACCGAAAACACTCAAAAAAATTAGAAACAAAAAAAGATGGCTCAAAAGCTTCATTTTTCCATCATTCTAATTTCTTTGTTCATCCTCTCATCAATTTCCCTATGTTTCTCCACCAAACCTAACAACAATGCCATAGATTTATGGTGCAGCCGAATGCCATATCCCGACGTATGCAAACACTTCTTCAATGGCCAATTCGACCCAAAAAAGCTTTCAGAATTCAAAAAAACAGCTCTTAAAATCGCCATGGATAGAGCTATAAGAGCTGAAACACTCACAAAAGAGTTAGGCAAAAAATGCCGTAACGAAAAAGAAAGAGCTGCATGGGCTGACTGTTTGGAGCTTTACCAAACCACAATCCTCCACCTCAACAAAACCTTCTCCGACGAAAAATGCTCTGACTTCGACATCCAAACATGGCTCAGCTCTGCCTTGACCGACCTCGAAACGTGCCGTGCCGGGTTCATCGACCTTAAGATAAAAGATTACGATGTGGTTTTCCCGTTTTTGGCGAACAATAATGTGACAAAGCTTATTAGTAATAGTTTGGCAATGAATAACTGCTCCGAGAGTGATGTGGGGTATAAGAGTAATGAAGGGTTTCCGAAGTGGGTTCGGCGTGGGGACCGGCGGCTGTTGCAGGCGGCGGAGCCTAAGGCGGATTTGGTGGTGGCACAGGACGGGTCGGGGAATTATAGGACGGTGCAAGCGGCGGTGGATGCGGCGGGGAAGAGGAAAGGGAGTGGGAGATTTGTGATACATATAAAGAGGGGAGTTTATAAAGAGAATGTAGTGATAAAGGTGAAGAATTTGATGCTTGTTGGAGATGGATTGAGGTATACAATTATTACTGGAAGTAGAAGTGTTGGTGGTGGTTCTACTACTTTCAACTCTGCTACTGTTGCTGTGACCGGTGAACGTTTCATTGCCCGTGGCATCACATTCCGCAACACCGCCGGACCTGAAAACCACCAAGCGGTAGCCCTTCGGTCAGGCTCCGATCTCTCAGTCTTCTTCCGGTGCGGTTTCGAAGGATACCAAGACACTCTCTACGCCCATTCACAAAGACAATTCTATAGAGAATGCTACATTTACGGCACGGTGGATTTCATCTTCGGCAACGCCGCCGTAGTTCTCCAGAACTGCATGATCTACGCAAGAAAACCAATCCCAGGGCAGCAATGCGTCGTCACCGCCCAAGGCCGAACCGACCCAAACCAAAACACAGGGATCTCAATCCACAATTCGAGAGTGATGGCGGCGGACGACCTAAAACCGGTCGTAGGGTCGGTTAGAACCTACTTGGGGAGGCCATGGAAGGAGTACTCTCGCACAGTTTACTTACAGAACTTCATGGATAACTTGGTGAATCCAGCAGGGTGGTTGGAATGGAGTGGTAATTTTGCATTGAATACATTGTATTATGGAGAGTTTAAGAATTTTGGGCCAGGTTCTTCTACTGCTAAGAGAGTGAAATGGAAGGGTTATAGAGTGATAACAAGTGCTACAGAAGCATCAAAATTTACTGTTGGGAATTTCATTGCTGGGAGATCATGGCTGCCTGGAACTGGAGTGCCATTCACTGCTGGGCTTTGAAATAATTATAGTGCATAAAAGTACATGAAGTTTGTGGTTAATTTAATTATTGGGTGATTGGAAGAGAGCATCTTGATTCATGTATCATTTGTTGGGGATTTCAATTTTATTATAGTACTTGTATCAATTATGTACCAATTCTTGGGGAAAGTTTATCTATGATTCTTTGTTGTTTCTTTCAGCCTAATTTGGATCTAATATATTTTTCTTGAGGGAATGAGTTTTTTTA

Coding sequence (CDS)

ATGGCTCAAAAGCTTCATTTTTCCATCATTCTAATTTCTTTGTTCATCCTCTCATCAATTTCCCTATGTTTCTCCACCAAACCTAACAACAATGCCATAGATTTATGGTGCAGCCGAATGCCATATCCCGACGTATGCAAACACTTCTTCAATGGCCAATTCGACCCAAAAAAGCTTTCAGAATTCAAAAAAACAGCTCTTAAAATCGCCATGGATAGAGCTATAAGAGCTGAAACACTCACAAAAGAGTTAGGCAAAAAATGCCGTAACGAAAAAGAAAGAGCTGCATGGGCTGACTGTTTGGAGCTTTACCAAACCACAATCCTCCACCTCAACAAAACCTTCTCCGACGAAAAATGCTCTGACTTCGACATCCAAACATGGCTCAGCTCTGCCTTGACCGACCTCGAAACGTGCCGTGCCGGGTTCATCGACCTTAAGATAAAAGATTACGATGTGGTTTTCCCGTTTTTGGCGAACAATAATGTGACAAAGCTTATTAGTAATAGTTTGGCAATGAATAACTGCTCCGAGAGTGATGTGGGGTATAAGAGTAATGAAGGGTTTCCGAAGTGGGTTCGGCGTGGGGACCGGCGGCTGTTGCAGGCGGCGGAGCCTAAGGCGGATTTGGTGGTGGCACAGGACGGGTCGGGGAATTATAGGACGGTGCAAGCGGCGGTGGATGCGGCGGGGAAGAGGAAAGGGAGTGGGAGATTTGTGATACATATAAAGAGGGGAGTTTATAAAGAGAATGTAGTGATAAAGGTGAAGAATTTGATGCTTGTTGGAGATGGATTGAGGTATACAATTATTACTGGAAGTAGAAGTGTTGGTGGTGGTTCTACTACTTTCAACTCTGCTACTGTTGCTGTGACCGGTGAACGTTTCATTGCCCGTGGCATCACATTCCGCAACACCGCCGGACCTGAAAACCACCAAGCGGTAGCCCTTCGGTCAGGCTCCGATCTCTCAGTCTTCTTCCGGTGCGGTTTCGAAGGATACCAAGACACTCTCTACGCCCATTCACAAAGACAATTCTATAGAGAATGCTACATTTACGGCACGGTGGATTTCATCTTCGGCAACGCCGCCGTAGTTCTCCAGAACTGCATGATCTACGCAAGAAAACCAATCCCAGGGCAGCAATGCGTCGTCACCGCCCAAGGCCGAACCGACCCAAACCAAAACACAGGGATCTCAATCCACAATTCGAGAGTGATGGCGGCGGACGACCTAAAACCGGTCGTAGGGTCGGTTAGAACCTACTTGGGGAGGCCATGGAAGGAGTACTCTCGCACAGTTTACTTACAGAACTTCATGGATAACTTGGTGAATCCAGCAGGGTGGTTGGAATGGAGTGGTAATTTTGCATTGAATACATTGTATTATGGAGAGTTTAAGAATTTTGGGCCAGGTTCTTCTACTGCTAAGAGAGTGAAATGGAAGGGTTATAGAGTGATAACAAGTGCTACAGAAGCATCAAAATTTACTGTTGGGAATTTCATTGCTGGGAGATCATGGCTGCCTGGAACTGGAGTGCCATTCACTGCTGGGCTTTGA

Protein sequence

MAQKLHFSIILISLFILSSISLCFSTKPNNNAIDLWCSRMPYPDVCKHFFNGQFDPKKLSEFKKTALKIAMDRAIRAETLTKELGKKCRNEKERAAWADCLELYQTTILHLNKTFSDEKCSDFDIQTWLSSALTDLETCRAGFIDLKIKDYDVVFPFLANNNVTKLISNSLAMNNCSESDVGYKSNEGFPKWVRRGDRRLLQAAEPKADLVVAQDGSGNYRTVQAAVDAAGKRKGSGRFVIHIKRGVYKENVVIKVKNLMLVGDGLRYTIITGSRSVGGGSTTFNSATVAVTGERFIARGITFRNTAGPENHQAVALRSGSDLSVFFRCGFEGYQDTLYAHSQRQFYRECYIYGTVDFIFGNAAVVLQNCMIYARKPIPGQQCVVTAQGRTDPNQNTGISIHNSRVMAADDLKPVVGSVRTYLGRPWKEYSRTVYLQNFMDNLVNPAGWLEWSGNFALNTLYYGEFKNFGPGSSTAKRVKWKGYRVITSATEASKFTVGNFIAGRSWLPGTGVPFTAGL

Homology

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BLAST of Bhi12G000009 vs. TAIR 10
Match: AT2G45220.1 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 594.3 bits (1531), Expect = 9.1e-170
Identity = 299/517 (57.83%), Postives = 389/517 (75.24%), Query Frame = 0


Query: 7   FSIILISLFILSSISLCFSTKPNNNAIDLWCSRMPYPDVCKHFF--NGQFDP-KKLSEFK 66
           F   +I+  IL  +     +  N   +  WCS+ P P  C++F   N   +P K  SEF 
Sbjct: 4   FRAYIINFVILCILVASTVSGYNQKDVKAWCSQTPNPKPCEYFLTHNSNNEPIKSESEFL 63

Query: 67  KTALKIAMDRAIRAETLTKELGKKCRNEKERAAWADCLELYQTTILHLNKTFS-DEKCSD 126
           K ++K+ +DRAI A+T    LG KCR+ +E+AAW DC++LY  T+  +N+T   + KCS 
Sbjct: 64  KISMKLVLDRAILAKTHAFTLGPKCRDTREKAAWEDCIKLYDLTVSKINETMDPNVKCSK 123

Query: 127 FDIQTWLSSALTDLETCRAGFIDLKIKDYDVVFPFLANNNVTKLISNSLAMNNCSESDVG 186
            D QTWLS+ALT+L+TCRAGF++L +   D+V P L +NNV+ L+ N+LA+N     +  
Sbjct: 124 LDAQTWLSTALTNLDTCRAGFLELGVT--DIVLP-LMSNNVSNLLCNTLAINKV-PFNYT 183

Query: 187 YKSNEGFPKWVRRGDRRLLQAAEPKADLVVAQDGSGNYRTVQAAVDAAGKRKGSGRFVIH 246
               +GFP WV+ GDR+LLQ++ PK + VVA+DGSGN++T++ A+DAA    GSGRFVI+
Sbjct: 184 PPEKDGFPSWVKPGDRKLLQSSTPKDNAVVAKDGSGNFKTIKEAIDAA---SGSGRFVIY 243

Query: 247 IKRGVYKENVVIKVKNLMLVGDGLRYTIITGSRSVGGGSTTFNSATVAVTGERFIARGIT 306
           +K+GVY EN+ I+ KN+ML GDG+  TIITGS+SVGGG+TTFNSATVA  G+ FIARGIT
Sbjct: 244 VKQGVYSENLEIRKKNVMLRGDGIGKTIITGSKSVGGGTTTFNSATVAAVGDGFIARGIT 303

Query: 307 FRNTAGPENHQAVALRSGSDLSVFFRCGFEGYQDTLYAHSQRQFYRECYIYGTVDFIFGN 366
           FRNTAG  N QAVALRSGSDLSVF++C FE YQDTLY HS RQFYR+C +YGTVDFIFGN
Sbjct: 304 FRNTAGASNEQAVALRSGSDLSVFYQCSFEAYQDTLYVHSNRQFYRDCDVYGTVDFIFGN 363

Query: 367 AAVVLQNCMIYARKPIPGQQCVVTAQGRTDPNQNTGISIHNSRVMAADDLKPVVGSVRTY 426
           AA VLQNC I+AR+P   +   +TAQGR+DPNQNTGI IHNSRV AA DL+PV+GS +TY
Sbjct: 364 AAAVLQNCNIFARRP-RSKTNTITAQGRSDPNQNTGIIIHNSRVTAASDLRPVLGSTKTY 423

Query: 427 LGRPWKEYSRTVYLQNFMDNLVNPAGWLEWSGNFALNTLYYGEFKNFGPGSSTAKRVKWK 486
           LGRPW++YSRTV+++  +D+L++P GWLEW GNFAL TL+Y EF+N GPG+ST+ RV W 
Sbjct: 424 LGRPWRQYSRTVFMKTSLDSLIDPRGWLEWDGNFALKTLFYAEFQNTGPGASTSGRVTWP 483

Query: 487 GYRVITSATEASKFTVGNFIAGRSWLPGTGVPFTAGL 520
           G+RV+ SA+EASKFTVG F+AG SW+P + VPFT+GL
Sbjct: 484 GFRVLGSASEASKFTVGTFLAGGSWIP-SSVPFTSGL 511

[Close]

BLAST of Bhi12G000009 vs. TAIR 10
Match: AT5G51490.1 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 577.8 bits (1488), Expect = 8.8e-165
Identity = 302/543 (55.62%), Postives = 388/543 (71.45%), Query Frame = 0


Query: 1   MAQKLHFSIILISLFILSSISLC---FSTKPNNNAIDLWCSRMPYPDVCKHFF---NGQF 60
           M QKL  SI+ + L +L  + LC    +T  + N+ D WC + PYPD CK +F   NG  
Sbjct: 4   MMQKL--SILFLHLILL--VLLCVHPLTTVADRNSTD-WCDKTPYPDPCKCYFKNHNGFQ 63

Query: 61  DPKKLSEFKKTALKIAMDRAIRAETLTKELGKKCRNEKERAAWADCLELYQTTILHLNKT 120
            P +LSEF+   ++ AMDRAI A       GK C + K++A  ADC++LY  TI+ LN+T
Sbjct: 64  QPTQLSEFRVMLVEAAMDRAISARAELTNSGKNCTDSKKQAVLADCIDLYGDTIMQLNRT 123

Query: 121 F--------SDEKCSDFDIQTWLSSALTDLETCRAGFIDLKIKDYDVVFPFLANNNVTKL 180
                    + + C+DFD QTWLS+ALT+ ETCR G  DL + D+  + P ++N  ++ L
Sbjct: 124 LHGVSPKAGAAKSCTDFDAQTWLSTALTNTETCRRGSSDLNVTDF--ITPIVSNTKISHL 183

Query: 181 ISNSLAMNNCSESDVGYKSN-----EGFPKWVRRGDRRLLQAAEPKADLVVAQDGSGNYR 240
           ISN LA+N    +  G K N     +GFP W+ R D+RLL+A   +A+LVVA+DGSG++ 
Sbjct: 184 ISNCLAVNGALLT-AGNKGNTTANQKGFPTWLSRKDKRLLRAV--RANLVVAKDGSGHFN 243

Query: 241 TVQAAVDAAGKRK-GSGRFVIHIKRGVYKENVVIKVKN--LMLVGDGLRYTIITGSRSVG 300
           TVQAA+D AG+RK  SGRFVI++KRG+Y+EN+ +++ N  +MLVGDG+R TIITG RSV 
Sbjct: 244 TVQAAIDVAGRRKVTSGRFVIYVKRGIYQENINVRLNNDDIMLVGDGMRSTIITGGRSVQ 303

Query: 301 GGSTTFNSATVAVTGERFIARGITFRNTAGPENHQAVALRSGSDLSVFFRCGFEGYQDTL 360
           GG TT+NSAT  + G  FIA+GITFRNTAGP   QAVALRS SDLS+F++C  EGYQDTL
Sbjct: 304 GGYTTYNSATAGIEGLHFIAKGITFRNTAGPAKGQAVALRSSSDLSIFYKCSIEGYQDTL 363

Query: 361 YAHSQRQFYRECYIYGTVDFIFGNAAVVLQNCMIYARKPIPGQQCVVTAQGRTDPNQNTG 420
             HSQRQFYRECYIYGTVDFIFGNAA V QNC+I  R+P+ GQ  V+TAQGR DP QNTG
Sbjct: 364 MVHSQRQFYRECYIYGTVDFIFGNAAAVFQNCLILPRRPLKGQANVITAQGRADPFQNTG 423

Query: 421 ISIHNSRVMAADDLKPVVGSVRTYLGRPWKEYSRTVYLQNFMDNLVNPAGWLEW--SGNF 480
           ISIHNSR++ A DLKPVVG+V+TY+GRPW ++SRTV LQ ++DN+V+P GW  W     F
Sbjct: 424 ISIHNSRILPAPDLKPVVGTVKTYMGRPWMKFSRTVVLQTYLDNVVSPVGWSPWIEGSVF 483

Query: 481 ALNTLYYGEFKNFGPGSSTAKRVKWKGYRVITSATEASKFTVGNFIAGRSWLPGTGVPFT 520
            L+TL+Y E+KN GP SST  RV WKG+ V+  A++AS FTVG FIAG +WLP TG+PFT
Sbjct: 484 GLDTLFYAEYKNTGPASSTRWRVSWKGFHVLGRASDASAFTVGKFIAGTAWLPRTGIPFT 536

[Close]

BLAST of Bhi12G000009 vs. TAIR 10
Match: AT5G51500.1 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 561.2 bits (1445), Expect = 8.5e-160
Identity = 287/538 (53.35%), Postives = 380/538 (70.63%), Query Frame = 0


Query: 1   MAQKLHF-SIILISLFILSSISLCFSTKPNNNAIDLWCSRMPYPDVCKHFF---NGQFDP 60
           M Q + F S+ L+ + +L    L      N+  ID WC + PYP  CK +F   +G   P
Sbjct: 5   MVQNISFLSLHLLLILLLCLRPLTTVADGNSTNIDGWCDKTPYPYPCKRYFIKHSGFRLP 64

Query: 61  KKLSEFKKTALKIAMDRAIRAETLTKELGKKCRNEKERAAWADCLELYQTTILHLNKTF- 120
            ++SEF+   ++ AMDRA+ A        K C + K++A  ADC+ LY  T++ LN+T  
Sbjct: 65  TQISEFRVLLVEAAMDRAVSAWDKLTNSSKNCTDFKKQAVLADCINLYGDTVMQLNRTLQ 124

Query: 121 -----SDEKCSDFDIQTWLSSALTDLETCRAGFIDLKIKDYDVVFPFLANNNVTKLISNS 180
                +  +C+DFD QTWLS+ALT+ ETCR G  DL + D+    P ++N  ++ LISN 
Sbjct: 125 GVSSKTGRRCTDFDAQTWLSTALTNTETCRRGSSDLNVSDFTT--PIVSNTKISHLISNC 184

Query: 181 LAMN----NCSESDVGYKSNEGFPKWVRRGDRRLLQAAEPKADLVVAQDGSGNYRTVQAA 240
           LA+N       ++D     ++GFP WV R +RRLLQ    +A+LVVA+DGSG+++TVQAA
Sbjct: 185 LAVNGALLTAGKNDSTTGDSKGFPTWVSRKERRLLQLQSVRANLVVAKDGSGHFKTVQAA 244

Query: 241 VDAAGKRK-GSGRFVIHIKRGVYKENVVIKVK--NLMLVGDGLRYTIITGSRSVGGGSTT 300
           +D AG+RK  SGRFVI++KRG+Y+EN+ +++   N+MLVGDG+RYTIITG RSV GG TT
Sbjct: 245 IDVAGRRKVTSGRFVIYVKRGIYQENLNVRLNNDNIMLVGDGMRYTIITGGRSVKGGYTT 304

Query: 301 FNSATVAVTGERFIARGITFRNTAGPENHQAVALRSGSDLSVFFRCGFEGYQDTLYAHSQ 360
           ++SAT  + G  FIA+GI F+NTAGP   QAVALRS SDLS+F+RC  EGYQDTL  HSQ
Sbjct: 305 YSSATAGIEGLHFIAKGIAFQNTAGPAKGQAVALRSSSDLSIFYRCSIEGYQDTLMVHSQ 364

Query: 361 RQFYRECYIYGTVDFIFGNAAVVLQNCMIYARKPIPGQQCVVTAQGRTDPNQNTGISIHN 420
           RQFYRECYIYGTVDFIFGNAAVV QNC+I  R P+ GQ  V+TAQGRTD  QNTGISIHN
Sbjct: 365 RQFYRECYIYGTVDFIFGNAAVVFQNCIILPRLPLKGQANVITAQGRTDLFQNTGISIHN 424

Query: 421 SRVMAADDLKPVVGSVRTYLGRPWKEYSRTVYLQNFMDNLVNPAGWLEWS--GNFALNTL 480
           S ++ A DLKPVV SV+TY+GRPW  YSRTV L+ ++D++V+P GW  W+    + L+TL
Sbjct: 425 SIIIPAPDLKPVVRSVKTYMGRPWMMYSRTVVLKTYIDSVVSPVGWSPWTKGSTYGLDTL 484

Query: 481 YYGEFKNFGPGSSTAKRVKWKGYRVITSATEASKFTVGNFIAGRSWLPGTGVPFTAGL 520
           +Y E+KN GP SST  RV+WKG+ V++ A++AS F+VG FIAG +WLPG+G+PFT+ L
Sbjct: 485 FYAEYKNIGPASSTRWRVRWKGFHVLSKASDASAFSVGKFIAGTAWLPGSGIPFTSEL 540

[Close]

BLAST of Bhi12G000009 vs. TAIR 10
Match: AT3G47400.1 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 532.3 bits (1370), Expect = 4.2e-151
Identity = 267/510 (52.35%), Postives = 356/510 (69.80%), Query Frame = 0


Query: 36  WCSRMPYPDVCKHFFNGQFD-------PKKLSEFKKTALKIAMDRAIRAETLTKELGKKC 95
           WC++ P+ + C ++F            P+  SEF +  +K+A+D+A+   + T + G  C
Sbjct: 87  WCNKTPHAETCNYYFRKSSQNNINLRPPRFRSEFLRMLVKVALDQAVITHSQTVKFGPSC 146

Query: 96  RNEKERAAWADCLELYQTTILHLNKTF--------SDEKCSDFDIQTWLSSALTDLETCR 155
            N + +AAW+DC+ L+Q T+  LN+T         SD KC+DFD QTWLS+A T++ETCR
Sbjct: 147 TNNQRKAAWSDCVNLFQNTVAQLNRTLKGLNPAASSDVKCTDFDAQTWLSTAQTNIETCR 206

Query: 156 AGFIDLKIKDYDVVFPFLANNNVTKLISNSLAMNN--CSESDVGYKSN--EGFPKWVRRG 215
           +G  DL + D+  V P ++N N++ LI N LA+N     + D    +N  E FP WV R 
Sbjct: 207 SGSEDLNVSDF--VMPVISNKNLSDLIGNCLAVNGVLMKQHDHTTTANHKEYFPSWVSRH 266

Query: 216 DRRLL---QAAEPKADLVVAQDGSGNYRTVQAAVDAAGKRKGSGRFVIHIKRGVYKENVV 275
           +RRLL     A+    LVVAQD SG++R++QAA++ A +R+   RFVI++K+GVY+EN+ 
Sbjct: 267 ERRLLVSASLAKSSPHLVVAQDRSGHFRSIQAAINFAARRRFKSRFVIYVKKGVYRENID 326

Query: 276 I--KVKNLMLVGDGLRYTIITGSRSVGGGSTTFNSATVAVTGERFIARGITFRNTAGPEN 335
           +     N+MLVGDG R TIIT  RSV  G TT+NSAT    G+RF+A+ +TF NTAGP  
Sbjct: 327 VGNDNHNIMLVGDGERKTIITSGRSVQHGYTTYNSATGGFGGQRFVAKDMTFINTAGPLR 386

Query: 336 HQAVALRSGSDLSVFFRCGFEGYQDTLYAHSQRQFYRECYIYGTVDFIFGNAAVVLQNCM 395
            QAVA+RS SDLSVF+R G  G+QDTLY HSQRQF+RECYI GT+DFIFGNAAVV QNCM
Sbjct: 387 GQAVAVRSSSDLSVFYRVGIHGFQDTLYIHSQRQFFRECYISGTIDFIFGNAAVVFQNCM 446

Query: 396 IYARKPIPGQQCVVTAQGRTDPNQNTGISIHNSRVMAADDLKPVVGSVRTYLGRPWKEYS 455
           I  R+P+ GQ  ++TAQGR DP QNTGI+IH+SR++AA DLKPV+ + +TYLGRPW+ YS
Sbjct: 447 ILVRRPLHGQANIITAQGRGDPFQNTGITIHSSRIIAASDLKPVIRAYKTYLGRPWQAYS 506

Query: 456 RTVYLQNFMDNLVNPAGWLEW--SGNFALNTLYYGEFKNFGPGSSTAKRVKWKGYRVITS 515
           R   ++ ++DN ++P GW  W    NFALNT++YGE+KNFGPGSST  RV+WKG+  ITS
Sbjct: 507 RVTIMKTYIDNSISPLGWSPWLRGSNFALNTVFYGEYKNFGPGSSTRWRVRWKGFHAITS 566

Query: 516 ATEASKFTVGNFIAGRSWLPGTGVPFTAGL 520
              AS+FTVG+ IAG SWLP TGVPF +GL
Sbjct: 567 TAVASRFTVGSLIAGGSWLPATGVPFKSGL 594

[Close]

BLAST of Bhi12G000009 vs. TAIR 10
Match: AT1G23200.1 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 444.5 bits (1142), Expect = 1.2e-124
Identity = 251/528 (47.54%), Postives = 326/528 (61.74%), Query Frame = 0


Query: 37  CSRMPYPDVCKHFFNGQFDPKKLSE-------FKKTALKIAMDRAIRAETLTKELGKKCR 96
           C + PYP VC H  +    P K  +       F    +   MD+A++   L   L +   
Sbjct: 38  CKQTPYPSVCDHHMSN--SPLKTLDDQTDGFTFHDLVVSSTMDQAVQLHRLVSSLKQHHS 97

Query: 97  NEKE-RAAWADCLELYQTTILHLN--KTFSDEKCSDFDIQTWLSSALTDLETCRAGFIDL 156
             K   +A  DCLELY+ TI  LN  +    +  S  D QT LS+A+ + +TCR GF D 
Sbjct: 98  LHKHATSALFDCLELYEDTIDQLNHSRRSYGQYSSPHDRQTSLSAAIANQDTCRNGFRDF 157

Query: 157 KI-KDYDVVFPFLANNNVTKLISNSLAMNNCSES---------DVGYK------------ 216
           K+   Y   FP   + N+TK ISNSLA+   +             G+             
Sbjct: 158 KLTSSYSKYFPVQFHRNLTKSISNSLAVTKAAAEAEAVAEKYPSTGFTKFSKQRSSAGGG 217

Query: 217 --------SNEGFPKWVRRGDRRLLQAAE--PKADLVVAQDGSGNYRTVQAAVDAAGK-R 276
                   S+E FP W    DR+LL+ ++   KADLVVA+DGSG+Y ++Q AV+AA K  
Sbjct: 218 SHRRLLLFSDEKFPSWFPLSDRKLLEDSKTTAKADLVVAKDGSGHYTSIQQAVNAAAKLP 277

Query: 277 KGSGRFVIHIKRGVYKENVVIK--VKNLMLVGDGLRYTIITGSRSVGGGSTTFNSATVAV 336
           + + R VI++K GVY+ENVVIK  +KN+M++GDG+  TI+TG+R+V  G+TTF SAT AV
Sbjct: 278 RRNQRLVIYVKAGVYRENVVIKKSIKNVMVIGDGIDSTIVTGNRNVQDGTTTFRSATFAV 337

Query: 337 TGERFIARGITFRNTAGPENHQAVALRSGSDLSVFFRCGFEGYQDTLYAHSQRQFYRECY 396
           +G  FIA+GITF NTAGPE HQAVALRS SD SVF+ C F+GYQDTLY HS RQF R C 
Sbjct: 338 SGNGFIAQGITFENTAGPEKHQAVALRSSSDFSVFYACSFKGYQDTLYLHSSRQFLRNCN 397

Query: 397 IYGTVDFIFGNAAVVLQNCMIYARKPIPGQQCVVTAQGRTDPNQNTGISIHNSRVMAADD 456
           IYGTVDFIFG+A  +LQNC IYARKP+ GQ+  +TAQ R +P++ TG  I +S V  A +
Sbjct: 398 IYGTVDFIFGDATAILQNCNIYARKPMSGQKNTITAQSRKEPDETTGFVIQSSTVATASE 457

Query: 457 LKPVVGSVRTYLGRPWKEYSRTVYLQNFMDNLVNPAGWLEWSGNFALNTLYYGEFKNFGP 516
                    TYLGRPW+ +SRTV+++  +  LV+PAGWL WSG+FAL+TLYYGE+ N G 
Sbjct: 458 ---------TYLGRPWRSHSRTVFMKCNLGALVSPAGWLPWSGSFALSTLYYGEYGNTGA 517

Query: 517 GSSTAKRVKWKGYRVITSATEASKFTVGNFIAGRSWLPGTGVPFTAGL 520
           G+S + RVKW GY VI + TEA KFTV NF+ G  W+  TGVP   GL
Sbjct: 518 GASVSGRVKWPGYHVIKTVTEAEKFTVENFLDGNYWITATGVPVNDGL 554

[Close]

BLAST of Bhi12G000009 vs. ExPASy Swiss-Prot
Match: O04887 (Pectinesterase 2 OS=Citrus sinensis OX=2711 GN=PECS-2.1 PE=2 SV=1)

HSP 1 Score: 633.3 bits (1632), Expect = 2.5e-180
Identity = 320/515 (62.14%), Postives = 394/515 (76.50%), Query Frame = 0


Query: 9   IILISLFILSSISLCFSTKPNNNAIDLWCSRMPYPDVCKHFFNGQFDP---KKLSEFKKT 68
           +I +SL + S     F   P    +  WC + P P  C++F   + D    K+ ++F K 
Sbjct: 6   LITVSLVLFSLSHTSFGYSPEE--VKSWCGKTPNPQPCEYFLTQKTDVTSIKQDTDFYKI 65

Query: 69  ALKIAMDRAIRAETLTKELGKKCRNEKERAAWADCLELYQTTILHLNKTF-SDEKCSDFD 128
           +L++A++RA  A++ T  LG KCRNE+E+AAW DC ELY+ T+L LN+T  S   C+  D
Sbjct: 66  SLQLALERATTAQSRTYTLGSKCRNEREKAAWEDCRELYELTVLKLNQTSNSSPGCTKVD 125

Query: 129 IQTWLSSALTDLETCRAGFIDLKIKDYDVVFPFLANNNVTKLISNSLAMNNCSESDVGYK 188
            QTWLS+ALT+LETCRA   DL + +Y  V P L +NNVTKLISN+L++N    ++  YK
Sbjct: 126 KQTWLSTALTNLETCRASLEDLGVPEY--VLPLL-SNNVTKLISNTLSLNKVPYNEPSYK 185

Query: 189 SNEGFPKWVRRGDRRLLQAAEPKADLVVAQDGSGNYRTVQAAVDAAGKRKGSGRFVIHIK 248
             +GFP WV+ GDR+LLQ   P+A++VVAQDGSGN +T+Q AV AA  R G  R+VI+IK
Sbjct: 186 --DGFPTWVKPGDRKLLQTT-PRANIVVAQDGSGNVKTIQEAV-AAASRAGGSRYVIYIK 245

Query: 249 RGVYKENVVIKVKNLMLVGDGLRYTIITGSRSVGGGSTTFNSATVAVTGERFIARGITFR 308
            G Y EN+ +K+KN+M VGDG+  TIITGS+SVGGG+TTF SATVAV G+ FIAR IT R
Sbjct: 246 AGTYNENIEVKLKNIMFVGDGIGKTIITGSKSVGGGATTFKSATVAVVGDNFIARDITIR 305

Query: 309 NTAGPENHQAVALRSGSDLSVFFRCGFEGYQDTLYAHSQRQFYRECYIYGTVDFIFGNAA 368
           NTAGP NHQAVALRSGSDLSVF+RC FEGYQDTLY HSQRQFYREC IYGTVDFIFGNAA
Sbjct: 306 NTAGPNNHQAVALRSGSDLSVFYRCSFEGYQDTLYVHSQRQFYRECDIYGTVDFIFGNAA 365

Query: 369 VVLQNCMIYARKPIPGQQCVVTAQGRTDPNQNTGISIHNSRVMAADDLKPVVGSVRTYLG 428
           VVLQNC I+ARKP P +   +TAQGRTDPNQ+TGI IHN RV AA DLKPV  SV+T+LG
Sbjct: 366 VVLQNCNIFARKP-PNRTNTLTAQGRTDPNQSTGIIIHNCRVTAASDLKPVQSSVKTFLG 425

Query: 429 RPWKEYSRTVYLQNFMDNLVNPAGWLEWSGNFALNTLYYGEFKNFGPGSSTAKRVKWKGY 488
           RPWK+YSRTVY++ F+D+L+NPAGW+EWSG+FALNTLYY E+ N GPGSSTA RVKW+GY
Sbjct: 426 RPWKQYSRTVYIKTFLDSLINPAGWMEWSGDFALNTLYYAEYMNTGPGSSTANRVKWRGY 485

Query: 489 RVITSATEASKFTVGNFIAGRSWLPGTGVPFTAGL 520
            V+TS ++ S+FTVGNFIAG SWLP T VPFT+GL
Sbjct: 486 HVLTSPSQVSQFTVGNFIAGNSWLPATNVPFTSGL 510

[Close]

BLAST of Bhi12G000009 vs. ExPASy Swiss-Prot
Match: O22149 (Probable pectinesterase/pectinesterase inhibitor 17 OS=Arabidopsis thaliana OX=3702 GN=PME17 PE=2 SV=2)

HSP 1 Score: 594.3 bits (1531), Expect = 1.3e-168
Identity = 299/517 (57.83%), Postives = 389/517 (75.24%), Query Frame = 0


Query: 7   FSIILISLFILSSISLCFSTKPNNNAIDLWCSRMPYPDVCKHFF--NGQFDP-KKLSEFK 66
           F   +I+  IL  +     +  N   +  WCS+ P P  C++F   N   +P K  SEF 
Sbjct: 4   FRAYIINFVILCILVASTVSGYNQKDVKAWCSQTPNPKPCEYFLTHNSNNEPIKSESEFL 63

Query: 67  KTALKIAMDRAIRAETLTKELGKKCRNEKERAAWADCLELYQTTILHLNKTFS-DEKCSD 126
           K ++K+ +DRAI A+T    LG KCR+ +E+AAW DC++LY  T+  +N+T   + KCS 
Sbjct: 64  KISMKLVLDRAILAKTHAFTLGPKCRDTREKAAWEDCIKLYDLTVSKINETMDPNVKCSK 123

Query: 127 FDIQTWLSSALTDLETCRAGFIDLKIKDYDVVFPFLANNNVTKLISNSLAMNNCSESDVG 186
            D QTWLS+ALT+L+TCRAGF++L +   D+V P L +NNV+ L+ N+LA+N     +  
Sbjct: 124 LDAQTWLSTALTNLDTCRAGFLELGVT--DIVLP-LMSNNVSNLLCNTLAINKV-PFNYT 183

Query: 187 YKSNEGFPKWVRRGDRRLLQAAEPKADLVVAQDGSGNYRTVQAAVDAAGKRKGSGRFVIH 246
               +GFP WV+ GDR+LLQ++ PK + VVA+DGSGN++T++ A+DAA    GSGRFVI+
Sbjct: 184 PPEKDGFPSWVKPGDRKLLQSSTPKDNAVVAKDGSGNFKTIKEAIDAA---SGSGRFVIY 243

Query: 247 IKRGVYKENVVIKVKNLMLVGDGLRYTIITGSRSVGGGSTTFNSATVAVTGERFIARGIT 306
           +K+GVY EN+ I+ KN+ML GDG+  TIITGS+SVGGG+TTFNSATVA  G+ FIARGIT
Sbjct: 244 VKQGVYSENLEIRKKNVMLRGDGIGKTIITGSKSVGGGTTTFNSATVAAVGDGFIARGIT 303

Query: 307 FRNTAGPENHQAVALRSGSDLSVFFRCGFEGYQDTLYAHSQRQFYRECYIYGTVDFIFGN 366
           FRNTAG  N QAVALRSGSDLSVF++C FE YQDTLY HS RQFYR+C +YGTVDFIFGN
Sbjct: 304 FRNTAGASNEQAVALRSGSDLSVFYQCSFEAYQDTLYVHSNRQFYRDCDVYGTVDFIFGN 363

Query: 367 AAVVLQNCMIYARKPIPGQQCVVTAQGRTDPNQNTGISIHNSRVMAADDLKPVVGSVRTY 426
           AA VLQNC I+AR+P   +   +TAQGR+DPNQNTGI IHNSRV AA DL+PV+GS +TY
Sbjct: 364 AAAVLQNCNIFARRP-RSKTNTITAQGRSDPNQNTGIIIHNSRVTAASDLRPVLGSTKTY 423

Query: 427 LGRPWKEYSRTVYLQNFMDNLVNPAGWLEWSGNFALNTLYYGEFKNFGPGSSTAKRVKWK 486
           LGRPW++YSRTV+++  +D+L++P GWLEW GNFAL TL+Y EF+N GPG+ST+ RV W 
Sbjct: 424 LGRPWRQYSRTVFMKTSLDSLIDPRGWLEWDGNFALKTLFYAEFQNTGPGASTSGRVTWP 483

Query: 487 GYRVITSATEASKFTVGNFIAGRSWLPGTGVPFTAGL 520
           G+RV+ SA+EASKFTVG F+AG SW+P + VPFT+GL
Sbjct: 484 GFRVLGSASEASKFTVGTFLAGGSWIP-SSVPFTSGL 511

[Close]

BLAST of Bhi12G000009 vs. ExPASy Swiss-Prot
Match: Q9FHN5 (Probable pectinesterase/pectinesterase inhibitor 59 OS=Arabidopsis thaliana OX=3702 GN=PME59 PE=2 SV=1)

HSP 1 Score: 577.8 bits (1488), Expect = 1.2e-163
Identity = 302/543 (55.62%), Postives = 388/543 (71.45%), Query Frame = 0


Query: 1   MAQKLHFSIILISLFILSSISLC---FSTKPNNNAIDLWCSRMPYPDVCKHFF---NGQF 60
           M QKL  SI+ + L +L  + LC    +T  + N+ D WC + PYPD CK +F   NG  
Sbjct: 4   MMQKL--SILFLHLILL--VLLCVHPLTTVADRNSTD-WCDKTPYPDPCKCYFKNHNGFQ 63

Query: 61  DPKKLSEFKKTALKIAMDRAIRAETLTKELGKKCRNEKERAAWADCLELYQTTILHLNKT 120
            P +LSEF+   ++ AMDRAI A       GK C + K++A  ADC++LY  TI+ LN+T
Sbjct: 64  QPTQLSEFRVMLVEAAMDRAISARAELTNSGKNCTDSKKQAVLADCIDLYGDTIMQLNRT 123

Query: 121 F--------SDEKCSDFDIQTWLSSALTDLETCRAGFIDLKIKDYDVVFPFLANNNVTKL 180
                    + + C+DFD QTWLS+ALT+ ETCR G  DL + D+  + P ++N  ++ L
Sbjct: 124 LHGVSPKAGAAKSCTDFDAQTWLSTALTNTETCRRGSSDLNVTDF--ITPIVSNTKISHL 183

Query: 181 ISNSLAMNNCSESDVGYKSN-----EGFPKWVRRGDRRLLQAAEPKADLVVAQDGSGNYR 240
           ISN LA+N    +  G K N     +GFP W+ R D+RLL+A   +A+LVVA+DGSG++ 
Sbjct: 184 ISNCLAVNGALLT-AGNKGNTTANQKGFPTWLSRKDKRLLRAV--RANLVVAKDGSGHFN 243

Query: 241 TVQAAVDAAGKRK-GSGRFVIHIKRGVYKENVVIKVKN--LMLVGDGLRYTIITGSRSVG 300
           TVQAA+D AG+RK  SGRFVI++KRG+Y+EN+ +++ N  +MLVGDG+R TIITG RSV 
Sbjct: 244 TVQAAIDVAGRRKVTSGRFVIYVKRGIYQENINVRLNNDDIMLVGDGMRSTIITGGRSVQ 303

Query: 301 GGSTTFNSATVAVTGERFIARGITFRNTAGPENHQAVALRSGSDLSVFFRCGFEGYQDTL 360
           GG TT+NSAT  + G  FIA+GITFRNTAGP   QAVALRS SDLS+F++C  EGYQDTL
Sbjct: 304 GGYTTYNSATAGIEGLHFIAKGITFRNTAGPAKGQAVALRSSSDLSIFYKCSIEGYQDTL 363

Query: 361 YAHSQRQFYRECYIYGTVDFIFGNAAVVLQNCMIYARKPIPGQQCVVTAQGRTDPNQNTG 420
             HSQRQFYRECYIYGTVDFIFGNAA V QNC+I  R+P+ GQ  V+TAQGR DP QNTG
Sbjct: 364 MVHSQRQFYRECYIYGTVDFIFGNAAAVFQNCLILPRRPLKGQANVITAQGRADPFQNTG 423

Query: 421 ISIHNSRVMAADDLKPVVGSVRTYLGRPWKEYSRTVYLQNFMDNLVNPAGWLEW--SGNF 480
           ISIHNSR++ A DLKPVVG+V+TY+GRPW ++SRTV LQ ++DN+V+P GW  W     F
Sbjct: 424 ISIHNSRILPAPDLKPVVGTVKTYMGRPWMKFSRTVVLQTYLDNVVSPVGWSPWIEGSVF 483

Query: 481 ALNTLYYGEFKNFGPGSSTAKRVKWKGYRVITSATEASKFTVGNFIAGRSWLPGTGVPFT 520
            L+TL+Y E+KN GP SST  RV WKG+ V+  A++AS FTVG FIAG +WLP TG+PFT
Sbjct: 484 GLDTLFYAEYKNTGPASSTRWRVSWKGFHVLGRASDASAFTVGKFIAGTAWLPRTGIPFT 536

[Close]

BLAST of Bhi12G000009 vs. ExPASy Swiss-Prot
Match: Q9FHN4 (Probable pectinesterase/pectinesterase inhibitor 60 OS=Arabidopsis thaliana OX=3702 GN=PME60 PE=2 SV=1)

HSP 1 Score: 561.2 bits (1445), Expect = 1.2e-158
Identity = 287/538 (53.35%), Postives = 380/538 (70.63%), Query Frame = 0


Query: 1   MAQKLHF-SIILISLFILSSISLCFSTKPNNNAIDLWCSRMPYPDVCKHFF---NGQFDP 60
           M Q + F S+ L+ + +L    L      N+  ID WC + PYP  CK +F   +G   P
Sbjct: 5   MVQNISFLSLHLLLILLLCLRPLTTVADGNSTNIDGWCDKTPYPYPCKRYFIKHSGFRLP 64

Query: 61  KKLSEFKKTALKIAMDRAIRAETLTKELGKKCRNEKERAAWADCLELYQTTILHLNKTF- 120
            ++SEF+   ++ AMDRA+ A        K C + K++A  ADC+ LY  T++ LN+T  
Sbjct: 65  TQISEFRVLLVEAAMDRAVSAWDKLTNSSKNCTDFKKQAVLADCINLYGDTVMQLNRTLQ 124

Query: 121 -----SDEKCSDFDIQTWLSSALTDLETCRAGFIDLKIKDYDVVFPFLANNNVTKLISNS 180
                +  +C+DFD QTWLS+ALT+ ETCR G  DL + D+    P ++N  ++ LISN 
Sbjct: 125 GVSSKTGRRCTDFDAQTWLSTALTNTETCRRGSSDLNVSDFTT--PIVSNTKISHLISNC 184

Query: 181 LAMN----NCSESDVGYKSNEGFPKWVRRGDRRLLQAAEPKADLVVAQDGSGNYRTVQAA 240
           LA+N       ++D     ++GFP WV R +RRLLQ    +A+LVVA+DGSG+++TVQAA
Sbjct: 185 LAVNGALLTAGKNDSTTGDSKGFPTWVSRKERRLLQLQSVRANLVVAKDGSGHFKTVQAA 244

Query: 241 VDAAGKRK-GSGRFVIHIKRGVYKENVVIKVK--NLMLVGDGLRYTIITGSRSVGGGSTT 300
           +D AG+RK  SGRFVI++KRG+Y+EN+ +++   N+MLVGDG+RYTIITG RSV GG TT
Sbjct: 245 IDVAGRRKVTSGRFVIYVKRGIYQENLNVRLNNDNIMLVGDGMRYTIITGGRSVKGGYTT 304

Query: 301 FNSATVAVTGERFIARGITFRNTAGPENHQAVALRSGSDLSVFFRCGFEGYQDTLYAHSQ 360
           ++SAT  + G  FIA+GI F+NTAGP   QAVALRS SDLS+F+RC  EGYQDTL  HSQ
Sbjct: 305 YSSATAGIEGLHFIAKGIAFQNTAGPAKGQAVALRSSSDLSIFYRCSIEGYQDTLMVHSQ 364

Query: 361 RQFYRECYIYGTVDFIFGNAAVVLQNCMIYARKPIPGQQCVVTAQGRTDPNQNTGISIHN 420
           RQFYRECYIYGTVDFIFGNAAVV QNC+I  R P+ GQ  V+TAQGRTD  QNTGISIHN
Sbjct: 365 RQFYRECYIYGTVDFIFGNAAVVFQNCIILPRLPLKGQANVITAQGRTDLFQNTGISIHN 424

Query: 421 SRVMAADDLKPVVGSVRTYLGRPWKEYSRTVYLQNFMDNLVNPAGWLEWS--GNFALNTL 480
           S ++ A DLKPVV SV+TY+GRPW  YSRTV L+ ++D++V+P GW  W+    + L+TL
Sbjct: 425 SIIIPAPDLKPVVRSVKTYMGRPWMMYSRTVVLKTYIDSVVSPVGWSPWTKGSTYGLDTL 484

Query: 481 YYGEFKNFGPGSSTAKRVKWKGYRVITSATEASKFTVGNFIAGRSWLPGTGVPFTAGL 520
           +Y E+KN GP SST  RV+WKG+ V++ A++AS F+VG FIAG +WLPG+G+PFT+ L
Sbjct: 485 FYAEYKNIGPASSTRWRVRWKGFHVLSKASDASAFSVGKFIAGTAWLPGSGIPFTSEL 540

[Close]

BLAST of Bhi12G000009 vs. ExPASy Swiss-Prot
Match: Q9STY3 (Probable pectinesterase/pectinesterase inhibitor 33 OS=Arabidopsis thaliana OX=3702 GN=PME33 PE=2 SV=1)

HSP 1 Score: 532.3 bits (1370), Expect = 6.0e-150
Identity = 267/510 (52.35%), Postives = 356/510 (69.80%), Query Frame = 0


Query: 36  WCSRMPYPDVCKHFFNGQFD-------PKKLSEFKKTALKIAMDRAIRAETLTKELGKKC 95
           WC++ P+ + C ++F            P+  SEF +  +K+A+D+A+   + T + G  C
Sbjct: 87  WCNKTPHAETCNYYFRKSSQNNINLRPPRFRSEFLRMLVKVALDQAVITHSQTVKFGPSC 146

Query: 96  RNEKERAAWADCLELYQTTILHLNKTF--------SDEKCSDFDIQTWLSSALTDLETCR 155
            N + +AAW+DC+ L+Q T+  LN+T         SD KC+DFD QTWLS+A T++ETCR
Sbjct: 147 TNNQRKAAWSDCVNLFQNTVAQLNRTLKGLNPAASSDVKCTDFDAQTWLSTAQTNIETCR 206

Query: 156 AGFIDLKIKDYDVVFPFLANNNVTKLISNSLAMNN--CSESDVGYKSN--EGFPKWVRRG 215
           +G  DL + D+  V P ++N N++ LI N LA+N     + D    +N  E FP WV R 
Sbjct: 207 SGSEDLNVSDF--VMPVISNKNLSDLIGNCLAVNGVLMKQHDHTTTANHKEYFPSWVSRH 266

Query: 216 DRRLL---QAAEPKADLVVAQDGSGNYRTVQAAVDAAGKRKGSGRFVIHIKRGVYKENVV 275
           +RRLL     A+    LVVAQD SG++R++QAA++ A +R+   RFVI++K+GVY+EN+ 
Sbjct: 267 ERRLLVSASLAKSSPHLVVAQDRSGHFRSIQAAINFAARRRFKSRFVIYVKKGVYRENID 326

Query: 276 I--KVKNLMLVGDGLRYTIITGSRSVGGGSTTFNSATVAVTGERFIARGITFRNTAGPEN 335
           +     N+MLVGDG R TIIT  RSV  G TT+NSAT    G+RF+A+ +TF NTAGP  
Sbjct: 327 VGNDNHNIMLVGDGERKTIITSGRSVQHGYTTYNSATGGFGGQRFVAKDMTFINTAGPLR 386

Query: 336 HQAVALRSGSDLSVFFRCGFEGYQDTLYAHSQRQFYRECYIYGTVDFIFGNAAVVLQNCM 395
            QAVA+RS SDLSVF+R G  G+QDTLY HSQRQF+RECYI GT+DFIFGNAAVV QNCM
Sbjct: 387 GQAVAVRSSSDLSVFYRVGIHGFQDTLYIHSQRQFFRECYISGTIDFIFGNAAVVFQNCM 446

Query: 396 IYARKPIPGQQCVVTAQGRTDPNQNTGISIHNSRVMAADDLKPVVGSVRTYLGRPWKEYS 455
           I  R+P+ GQ  ++TAQGR DP QNTGI+IH+SR++AA DLKPV+ + +TYLGRPW+ YS
Sbjct: 447 ILVRRPLHGQANIITAQGRGDPFQNTGITIHSSRIIAASDLKPVIRAYKTYLGRPWQAYS 506

Query: 456 RTVYLQNFMDNLVNPAGWLEW--SGNFALNTLYYGEFKNFGPGSSTAKRVKWKGYRVITS 515
           R   ++ ++DN ++P GW  W    NFALNT++YGE+KNFGPGSST  RV+WKG+  ITS
Sbjct: 507 RVTIMKTYIDNSISPLGWSPWLRGSNFALNTVFYGEYKNFGPGSSTRWRVRWKGFHAITS 566

Query: 516 ATEASKFTVGNFIAGRSWLPGTGVPFTAGL 520
              AS+FTVG+ IAG SWLP TGVPF +GL
Sbjct: 567 TAVASRFTVGSLIAGGSWLPATGVPFKSGL 594

[Close]

BLAST of Bhi12G000009 vs. NCBI nr
Match: XP_038875884.1 (pectinesterase 2-like [Benincasa hispida])

HSP 1 Score: 1054.3 bits (2725), Expect = 3.4e-304
Identity = 519/519 (100.00%), Postives = 519/519 (100.00%), Query Frame = 0


Query: 1   MAQKLHFSIILISLFILSSISLCFSTKPNNNAIDLWCSRMPYPDVCKHFFNGQFDPKKLS 60
           MAQKLHFSIILISLFILSSISLCFSTKPNNNAIDLWCSRMPYPDVCKHFFNGQFDPKKLS
Sbjct: 1   MAQKLHFSIILISLFILSSISLCFSTKPNNNAIDLWCSRMPYPDVCKHFFNGQFDPKKLS 60

Query: 61  EFKKTALKIAMDRAIRAETLTKELGKKCRNEKERAAWADCLELYQTTILHLNKTFSDEKC 120
           EFKKTALKIAMDRAIRAETLTKELGKKCRNEKERAAWADCLELYQTTILHLNKTFSDEKC
Sbjct: 61  EFKKTALKIAMDRAIRAETLTKELGKKCRNEKERAAWADCLELYQTTILHLNKTFSDEKC 120

Query: 121 SDFDIQTWLSSALTDLETCRAGFIDLKIKDYDVVFPFLANNNVTKLISNSLAMNNCSESD 180
           SDFDIQTWLSSALTDLETCRAGFIDLKIKDYDVVFPFLANNNVTKLISNSLAMNNCSESD
Sbjct: 121 SDFDIQTWLSSALTDLETCRAGFIDLKIKDYDVVFPFLANNNVTKLISNSLAMNNCSESD 180

Query: 181 VGYKSNEGFPKWVRRGDRRLLQAAEPKADLVVAQDGSGNYRTVQAAVDAAGKRKGSGRFV 240
           VGYKSNEGFPKWVRRGDRRLLQAAEPKADLVVAQDGSGNYRTVQAAVDAAGKRKGSGRFV
Sbjct: 181 VGYKSNEGFPKWVRRGDRRLLQAAEPKADLVVAQDGSGNYRTVQAAVDAAGKRKGSGRFV 240

Query: 241 IHIKRGVYKENVVIKVKNLMLVGDGLRYTIITGSRSVGGGSTTFNSATVAVTGERFIARG 300
           IHIKRGVYKENVVIKVKNLMLVGDGLRYTIITGSRSVGGGSTTFNSATVAVTGERFIARG
Sbjct: 241 IHIKRGVYKENVVIKVKNLMLVGDGLRYTIITGSRSVGGGSTTFNSATVAVTGERFIARG 300

Query: 301 ITFRNTAGPENHQAVALRSGSDLSVFFRCGFEGYQDTLYAHSQRQFYRECYIYGTVDFIF 360
           ITFRNTAGPENHQAVALRSGSDLSVFFRCGFEGYQDTLYAHSQRQFYRECYIYGTVDFIF
Sbjct: 301 ITFRNTAGPENHQAVALRSGSDLSVFFRCGFEGYQDTLYAHSQRQFYRECYIYGTVDFIF 360

Query: 361 GNAAVVLQNCMIYARKPIPGQQCVVTAQGRTDPNQNTGISIHNSRVMAADDLKPVVGSVR 420
           GNAAVVLQNCMIYARKPIPGQQCVVTAQGRTDPNQNTGISIHNSRVMAADDLKPVVGSVR
Sbjct: 361 GNAAVVLQNCMIYARKPIPGQQCVVTAQGRTDPNQNTGISIHNSRVMAADDLKPVVGSVR 420

Query: 421 TYLGRPWKEYSRTVYLQNFMDNLVNPAGWLEWSGNFALNTLYYGEFKNFGPGSSTAKRVK 480
           TYLGRPWKEYSRTVYLQNFMDNLVNPAGWLEWSGNFALNTLYYGEFKNFGPGSSTAKRVK
Sbjct: 421 TYLGRPWKEYSRTVYLQNFMDNLVNPAGWLEWSGNFALNTLYYGEFKNFGPGSSTAKRVK 480

Query: 481 WKGYRVITSATEASKFTVGNFIAGRSWLPGTGVPFTAGL 520
           WKGYRVITSATEASKFTVGNFIAGRSWLPGTGVPFTAGL
Sbjct: 481 WKGYRVITSATEASKFTVGNFIAGRSWLPGTGVPFTAGL 519

[Close]

BLAST of Bhi12G000009 vs. NCBI nr
Match: XP_008436965.1 (PREDICTED: pectinesterase 2-like [Cucumis melo])

HSP 1 Score: 948.3 bits (2450), Expect = 2.7e-272
Identity = 468/520 (90.00%), Postives = 493/520 (94.81%), Query Frame = 0


Query: 1   MAQKLHFSIILISLFILSSISLCFSTKPNNNAIDLWCSRMPYPDVCKHFFN-GQFDPKKL 60
           MAQKLHFSI+L SLFILSS SL FSTK N  AIDLWCSR PYPDVCK+FFN GQFDPK L
Sbjct: 1   MAQKLHFSILLFSLFILSSTSLPFSTKTNKKAIDLWCSRTPYPDVCKNFFNDGQFDPKNL 60

Query: 61  SEFKKTALKIAMDRAIRAETLTKELGKKCRNEKERAAWADCLELYQTTILHLNKTFSDEK 120
            + +KTALKIAM+RAIR ETLTK LG+KCRN+KERAAWADCLELYQTTILHLNKTFSD  
Sbjct: 61  LDIQKTALKIAMERAIRTETLTKALGQKCRNKKERAAWADCLELYQTTILHLNKTFSDH- 120

Query: 121 CSDFDIQTWLSSALTDLETCRAGFIDLKIKDYDVVFPFLANNNVTKLISNSLAMNNCSES 180
           CS+FDIQTWLSSALT+L+TCRAGF+DL IKDY VVFPFLANNN+TKLISNSLAMNNCSES
Sbjct: 121 CSNFDIQTWLSSALTNLDTCRAGFVDLGIKDYGVVFPFLANNNITKLISNSLAMNNCSES 180

Query: 181 DVGYKSNEGFPKWVRRGDRRLLQAAEPKADLVVAQDGSGNYRTVQAAVDAAGKRKGSGRF 240
           D G  S+EGFPKWVR GDRRLLQAAEPKADLVVAQDGSGNY+TVQAAVDAAGKRKGSGRF
Sbjct: 181 DEGNTSDEGFPKWVRGGDRRLLQAAEPKADLVVAQDGSGNYKTVQAAVDAAGKRKGSGRF 240

Query: 241 VIHIKRGVYKENVVIKVKNLMLVGDGLRYTIITGSRSVGGGSTTFNSATVAVTGERFIAR 300
           VI IK+GVYKENVVIKVKNLMLVGDGLRYTIITGSRSVGGGSTTFNSATVAVTGERFIAR
Sbjct: 241 VIRIKKGVYKENVVIKVKNLMLVGDGLRYTIITGSRSVGGGSTTFNSATVAVTGERFIAR 300

Query: 301 GITFRNTAGPENHQAVALRSGSDLSVFFRCGFEGYQDTLYAHSQRQFYRECYIYGTVDFI 360
           GITFRNTAGP+NHQAVALRSG+DLSVFFRCGFEGYQDTLYAHSQRQFYRECYIYGT+DFI
Sbjct: 301 GITFRNTAGPQNHQAVALRSGADLSVFFRCGFEGYQDTLYAHSQRQFYRECYIYGTIDFI 360

Query: 361 FGNAAVVLQNCMIYARKPIPGQQCVVTAQGRTDPNQNTGISIHNSRVMAADDLKPVVGSV 420
           FGNAAVVLQNCMIYARKP+ GQQCVVTAQGRTDPNQNTGISIHNSRVMAADDLKPV+ SV
Sbjct: 361 FGNAAVVLQNCMIYARKPMQGQQCVVTAQGRTDPNQNTGISIHNSRVMAADDLKPVIKSV 420

Query: 421 RTYLGRPWKEYSRTVYLQNFMDNLVNPAGWLEWSGNFALNTLYYGEFKNFGPGSSTAKRV 480
           RTYLGRPWKEYSRTVYLQ+FM++LVNPAGWLEWSGNFALNTLYYGEFKN+GPGSSTA RV
Sbjct: 421 RTYLGRPWKEYSRTVYLQSFMESLVNPAGWLEWSGNFALNTLYYGEFKNYGPGSSTANRV 480

Query: 481 KWKGYRVITSATEASKFTVGNFIAGRSWLPGTGVPFTAGL 520
           KWKGYRVITSA EA+KFTVGNFIAG SWLPGTGVPFTAGL
Sbjct: 481 KWKGYRVITSANEAAKFTVGNFIAGNSWLPGTGVPFTAGL 519

[Close]

BLAST of Bhi12G000009 vs. NCBI nr
Match: XP_004143735.1 (pectinesterase 2 [Cucumis sativus] >KGN50327.1 hypothetical protein Csa_000416 [Cucumis sativus])

HSP 1 Score: 938.7 bits (2425), Expect = 2.1e-269
Identity = 458/520 (88.08%), Postives = 489/520 (94.04%), Query Frame = 0


Query: 1   MAQKLHFSIILISLFILSSISLCFSTKPNNNAIDLWCSRMPYPDVCKHFF-NGQFDPKKL 60
           MAQKLHFSIIL S+FILSS SL FSTK NN AI+LWCSR PYPDVCKHFF NG+FDP+ L
Sbjct: 1   MAQKLHFSIILFSMFILSSSSLPFSTKTNNKAIELWCSRTPYPDVCKHFFNNGEFDPRNL 60

Query: 61  SEFKKTALKIAMDRAIRAETLTKELGKKCRNEKERAAWADCLELYQTTILHLNKTFSDEK 120
            + KK ALKIAM+RA++ ETLTK LG+KCRN+KERAAWADCLELYQTTILHLNKTFSD+ 
Sbjct: 61  LDIKKAALKIAMERAMKTETLTKALGQKCRNKKERAAWADCLELYQTTILHLNKTFSDKN 120

Query: 121 CSDFDIQTWLSSALTDLETCRAGFIDLKIKDYDVVFPFLANNNVTKLISNSLAMNNCSES 180
           CS+FDIQTWLSSALT+L TCRAGF+DL IKDY VVFPFL NNN+TKLISNSLAMNNCSES
Sbjct: 121 CSNFDIQTWLSSALTNLHTCRAGFVDLGIKDYGVVFPFLENNNITKLISNSLAMNNCSES 180

Query: 181 DVGYKSNEGFPKWVRRGDRRLLQAAEPKADLVVAQDGSGNYRTVQAAVDAAGKRKGSGRF 240
           D G  S+EGFPKW+  GDRRLLQAAEPKADLVVAQDGSGNY+TVQAAVDAAGKRKGSGRF
Sbjct: 181 DEGNTSDEGFPKWLHGGDRRLLQAAEPKADLVVAQDGSGNYKTVQAAVDAAGKRKGSGRF 240

Query: 241 VIHIKRGVYKENVVIKVKNLMLVGDGLRYTIITGSRSVGGGSTTFNSATVAVTGERFIAR 300
           VI +K+GVYKENVVIKVKNLMLVGDGL+YTIITGSRSVGGGSTTFNSATVAVTGERFIAR
Sbjct: 241 VIRVKKGVYKENVVIKVKNLMLVGDGLKYTIITGSRSVGGGSTTFNSATVAVTGERFIAR 300

Query: 301 GITFRNTAGPENHQAVALRSGSDLSVFFRCGFEGYQDTLYAHSQRQFYRECYIYGTVDFI 360
           GITFRNTAGP+NHQAVALRSG+DLSVFFRCGFEGYQDTLY HSQRQFYRECYIYGTVDFI
Sbjct: 301 GITFRNTAGPQNHQAVALRSGADLSVFFRCGFEGYQDTLYVHSQRQFYRECYIYGTVDFI 360

Query: 361 FGNAAVVLQNCMIYARKPIPGQQCVVTAQGRTDPNQNTGISIHNSRVMAADDLKPVVGSV 420
           FGN+AVVLQNCMIYARKP+ GQQCVVTAQGRTDPNQNTGISIHNSRVMA DDLKPV+ SV
Sbjct: 361 FGNSAVVLQNCMIYARKPMQGQQCVVTAQGRTDPNQNTGISIHNSRVMATDDLKPVIKSV 420

Query: 421 RTYLGRPWKEYSRTVYLQNFMDNLVNPAGWLEWSGNFALNTLYYGEFKNFGPGSSTAKRV 480
           RTYLGRPWKEYSRTVYLQ+FMD+LVNPAGWLEWSGNFALNTLYYGEFKN GPGSSTA RV
Sbjct: 421 RTYLGRPWKEYSRTVYLQSFMDSLVNPAGWLEWSGNFALNTLYYGEFKNSGPGSSTANRV 480

Query: 481 KWKGYRVITSATEASKFTVGNFIAGRSWLPGTGVPFTAGL 520
           KWKGYRVITSA+EA+KFTVG+FIAG SWLPGTGVPFTAGL
Sbjct: 481 KWKGYRVITSASEAAKFTVGSFIAGNSWLPGTGVPFTAGL 520

[Close]

BLAST of Bhi12G000009 vs. NCBI nr
Match: XP_022996028.1 (pectinesterase-like [Cucurbita maxima])

HSP 1 Score: 872.5 bits (2253), Expect = 1.9e-249
Identity = 435/522 (83.33%), Postives = 469/522 (89.85%), Query Frame = 0


Query: 1   MAQKLHFSIILISLFILSSISLCFSTKPNNNAIDLWCSRMPYPDVCKHFFNGQFDPKKLS 60
           MAQ LHF I++IS  IL SIS  FS     NA DLWCS+ PYPDVCK FF  +F+PK L+
Sbjct: 1   MAQNLHFPILVISFTILLSISPSFSAP---NAKDLWCSQTPYPDVCKSFFKAKFNPKNLA 60

Query: 61  EFKKTALKIAMDRAIRAETLTKELGKKCRNEKERAAWADCLELYQTTILHLNKTFSDEKC 120
           EFKKTA KIAM+RA+ AETLTK   +KCR  KE+ AWADCLELYQ TILHLN+TFSD  C
Sbjct: 61  EFKKTAFKIAMERALTAETLTKRHAQKCRTPKEKGAWADCLELYQDTILHLNRTFSDNYC 120

Query: 121 SDFDIQTWLSSALTDLETCRAGFIDLKIKDYDVVFPFLA-NNNVTKLISNSLAMNNCSES 180
           SDFDIQTWLSSALT+LETCRAGF+DL IKDYDVVFPFL+ +NNVTKLI NSLAMNNCSE+
Sbjct: 121 SDFDIQTWLSSALTNLETCRAGFVDLNIKDYDVVFPFLSRSNNVTKLIRNSLAMNNCSET 180

Query: 181 DVGYKSNEGFPKWVRRGDRRLLQAAEPKADLVVAQDGSGNYRTVQAAVDAAGKRKGSGRF 240
           + GY  + GFP WV+ G+RRLLQAA PKADLVVA DGSGNY+TVQAAVDAA KRKGSGRF
Sbjct: 181 ETGY--HGGFPTWVKAGERRLLQAAVPKADLVVALDGSGNYKTVQAAVDAAAKRKGSGRF 240

Query: 241 VIHIKRGVYKENVVI--KVKNLMLVGDGLRYTIITGSRSVGGGSTTFNSATVAVTGERFI 300
           VIH+K+GVYKENVVI  KVKNLMLVGDGL+YTIITGSRSVGGGSTTFNSATVAVTGE FI
Sbjct: 241 VIHVKKGVYKENVVIGNKVKNLMLVGDGLKYTIITGSRSVGGGSTTFNSATVAVTGEGFI 300

Query: 301 ARGITFRNTAGPENHQAVALRSGSDLSVFFRCGFEGYQDTLYAHSQRQFYRECYIYGTVD 360
           ARGITFRNTAGPENHQAVALRSGSDLSVF+RC FEGYQDTLY HSQRQFYRECYIYGTVD
Sbjct: 301 ARGITFRNTAGPENHQAVALRSGSDLSVFYRCSFEGYQDTLYVHSQRQFYRECYIYGTVD 360

Query: 361 FIFGNAAVVLQNCMIYARKPIPGQQCVVTAQGRTDPNQNTGISIHNSRVMAADDLKPVVG 420
           FIFGNAAVVLQNCMIYARKP+ GQQCVVTAQGR+DPNQNTGISIHNSRVMA  DL+PV+ 
Sbjct: 361 FIFGNAAVVLQNCMIYARKPMSGQQCVVTAQGRSDPNQNTGISIHNSRVMATADLQPVLS 420

Query: 421 SVRTYLGRPWKEYSRTVYLQNFMDNLVNPAGWLEWSGNFALNTLYYGEFKNFGPGSSTAK 480
           SVRTYLGRPW+EYSRTVYLQ FMD+LVNPAGWLEWSG+FALNTLYYGEFKNFGPGSSTAK
Sbjct: 421 SVRTYLGRPWREYSRTVYLQCFMDSLVNPAGWLEWSGDFALNTLYYGEFKNFGPGSSTAK 480

Query: 481 RVKWKGYRVITSATEASKFTVGNFIAGRSWLPGTGVPFTAGL 520
           RVKWKGYRVITSA EASKFTVG+FIAG+SWLP TGVPFTAGL
Sbjct: 481 RVKWKGYRVITSAAEASKFTVGSFIAGKSWLPATGVPFTAGL 517

[Close]

BLAST of Bhi12G000009 vs. NCBI nr
Match: XP_023534348.1 (pectinesterase-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 864.4 bits (2232), Expect = 5.1e-247
Identity = 430/522 (82.38%), Postives = 467/522 (89.46%), Query Frame = 0


Query: 1   MAQKLHFSIILISLFILSSISLCFSTKPNNNAIDLWCSRMPYPDVCKHFFNGQFDPKKLS 60
           MAQ  HFSI+LIS+ IL SIS   S     NA +LWCS+ PYPD+CK FFN +F+PK L+
Sbjct: 1   MAQNRHFSILLISMSILLSISPSLSAP---NAKELWCSQTPYPDICKSFFNAKFNPKNLA 60

Query: 61  EFKKTALKIAMDRAIRAETLTKELGKKCRNEKERAAWADCLELYQTTILHLNKTFSDEKC 120
           EFKK A KIAM+RA+ AE+LTK   +KCR  KE+ AWADCLELYQ TIL LN+TFSD  C
Sbjct: 61  EFKKAAFKIAMERALTAESLTKRHAQKCRTPKEKGAWADCLELYQDTILQLNRTFSDNYC 120

Query: 121 SDFDIQTWLSSALTDLETCRAGFIDLKIKDYDVVFPFLA-NNNVTKLISNSLAMNNCSES 180
           SDFDIQTWLSSALT+L+TCRAGF+DL IKDYDVVFPFL  +NNVTKLI NSLAMNNCSE+
Sbjct: 121 SDFDIQTWLSSALTNLDTCRAGFVDLNIKDYDVVFPFLGRSNNVTKLIRNSLAMNNCSET 180

Query: 181 DVGYKSNEGFPKWVRRGDRRLLQAAEPKADLVVAQDGSGNYRTVQAAVDAAGKRKGSGRF 240
           + GY  + GFP WV+ G+RRLLQAA PK DLVVA DGSGNY+TVQAAVDAA KRKGSGRF
Sbjct: 181 ETGY--HGGFPTWVKAGERRLLQAAVPKPDLVVALDGSGNYKTVQAAVDAAAKRKGSGRF 240

Query: 241 VIHIKRGVYKENVVI--KVKNLMLVGDGLRYTIITGSRSVGGGSTTFNSATVAVTGERFI 300
           VIH+K+GVYKENVVI  KVKNLMLVGDGL+YTIITGSRSVGGGSTTFNSATVAVTGE FI
Sbjct: 241 VIHVKKGVYKENVVIGNKVKNLMLVGDGLKYTIITGSRSVGGGSTTFNSATVAVTGEGFI 300

Query: 301 ARGITFRNTAGPENHQAVALRSGSDLSVFFRCGFEGYQDTLYAHSQRQFYRECYIYGTVD 360
           ARGITFRNTAGP+NHQAVALRSGSDLSVF+RCGFEGYQDTLY HSQRQFYRECYIYGTVD
Sbjct: 301 ARGITFRNTAGPQNHQAVALRSGSDLSVFYRCGFEGYQDTLYVHSQRQFYRECYIYGTVD 360

Query: 361 FIFGNAAVVLQNCMIYARKPIPGQQCVVTAQGRTDPNQNTGISIHNSRVMAADDLKPVVG 420
           FIFGNAAVVLQNCMIYARKP+ GQQCVVTAQGR+DPNQNTGISIHNSRVMA  DL+PV+ 
Sbjct: 361 FIFGNAAVVLQNCMIYARKPMSGQQCVVTAQGRSDPNQNTGISIHNSRVMATADLQPVLS 420

Query: 421 SVRTYLGRPWKEYSRTVYLQNFMDNLVNPAGWLEWSGNFALNTLYYGEFKNFGPGSSTAK 480
           SVRTYLGRPWKEYSRTVYLQ FMD+LVNPAGWLEWSG+FALNTLYYGEFKNFGPGSSTAK
Sbjct: 421 SVRTYLGRPWKEYSRTVYLQCFMDSLVNPAGWLEWSGDFALNTLYYGEFKNFGPGSSTAK 480

Query: 481 RVKWKGYRVITSATEASKFTVGNFIAGRSWLPGTGVPFTAGL 520
           RVKWKGYRVITSA EASKFTVG+FIAG+SWLP TGVPFTAGL
Sbjct: 481 RVKWKGYRVITSAAEASKFTVGSFIAGKSWLPATGVPFTAGL 517

[Close]

BLAST of Bhi12G000009 vs. ExPASy TrEMBL
Match: A0A1S3ASJ4 (Pectinesterase OS=Cucumis melo OX=3656 GN=LOC103482536 PE=3 SV=1)

HSP 1 Score: 948.3 bits (2450), Expect = 1.3e-272
Identity = 468/520 (90.00%), Postives = 493/520 (94.81%), Query Frame = 0


Query: 1   MAQKLHFSIILISLFILSSISLCFSTKPNNNAIDLWCSRMPYPDVCKHFFN-GQFDPKKL 60
           MAQKLHFSI+L SLFILSS SL FSTK N  AIDLWCSR PYPDVCK+FFN GQFDPK L
Sbjct: 1   MAQKLHFSILLFSLFILSSTSLPFSTKTNKKAIDLWCSRTPYPDVCKNFFNDGQFDPKNL 60

Query: 61  SEFKKTALKIAMDRAIRAETLTKELGKKCRNEKERAAWADCLELYQTTILHLNKTFSDEK 120
            + +KTALKIAM+RAIR ETLTK LG+KCRN+KERAAWADCLELYQTTILHLNKTFSD  
Sbjct: 61  LDIQKTALKIAMERAIRTETLTKALGQKCRNKKERAAWADCLELYQTTILHLNKTFSDH- 120

Query: 121 CSDFDIQTWLSSALTDLETCRAGFIDLKIKDYDVVFPFLANNNVTKLISNSLAMNNCSES 180
           CS+FDIQTWLSSALT+L+TCRAGF+DL IKDY VVFPFLANNN+TKLISNSLAMNNCSES
Sbjct: 121 CSNFDIQTWLSSALTNLDTCRAGFVDLGIKDYGVVFPFLANNNITKLISNSLAMNNCSES 180

Query: 181 DVGYKSNEGFPKWVRRGDRRLLQAAEPKADLVVAQDGSGNYRTVQAAVDAAGKRKGSGRF 240
           D G  S+EGFPKWVR GDRRLLQAAEPKADLVVAQDGSGNY+TVQAAVDAAGKRKGSGRF
Sbjct: 181 DEGNTSDEGFPKWVRGGDRRLLQAAEPKADLVVAQDGSGNYKTVQAAVDAAGKRKGSGRF 240

Query: 241 VIHIKRGVYKENVVIKVKNLMLVGDGLRYTIITGSRSVGGGSTTFNSATVAVTGERFIAR 300
           VI IK+GVYKENVVIKVKNLMLVGDGLRYTIITGSRSVGGGSTTFNSATVAVTGERFIAR
Sbjct: 241 VIRIKKGVYKENVVIKVKNLMLVGDGLRYTIITGSRSVGGGSTTFNSATVAVTGERFIAR 300

Query: 301 GITFRNTAGPENHQAVALRSGSDLSVFFRCGFEGYQDTLYAHSQRQFYRECYIYGTVDFI 360
           GITFRNTAGP+NHQAVALRSG+DLSVFFRCGFEGYQDTLYAHSQRQFYRECYIYGT+DFI
Sbjct: 301 GITFRNTAGPQNHQAVALRSGADLSVFFRCGFEGYQDTLYAHSQRQFYRECYIYGTIDFI 360

Query: 361 FGNAAVVLQNCMIYARKPIPGQQCVVTAQGRTDPNQNTGISIHNSRVMAADDLKPVVGSV 420
           FGNAAVVLQNCMIYARKP+ GQQCVVTAQGRTDPNQNTGISIHNSRVMAADDLKPV+ SV
Sbjct: 361 FGNAAVVLQNCMIYARKPMQGQQCVVTAQGRTDPNQNTGISIHNSRVMAADDLKPVIKSV 420

Query: 421 RTYLGRPWKEYSRTVYLQNFMDNLVNPAGWLEWSGNFALNTLYYGEFKNFGPGSSTAKRV 480
           RTYLGRPWKEYSRTVYLQ+FM++LVNPAGWLEWSGNFALNTLYYGEFKN+GPGSSTA RV
Sbjct: 421 RTYLGRPWKEYSRTVYLQSFMESLVNPAGWLEWSGNFALNTLYYGEFKNYGPGSSTANRV 480

Query: 481 KWKGYRVITSATEASKFTVGNFIAGRSWLPGTGVPFTAGL 520
           KWKGYRVITSA EA+KFTVGNFIAG SWLPGTGVPFTAGL
Sbjct: 481 KWKGYRVITSANEAAKFTVGNFIAGNSWLPGTGVPFTAGL 519

[Close]

BLAST of Bhi12G000009 vs. ExPASy TrEMBL
Match: A0A0A0KPG9 (Pectinesterase OS=Cucumis sativus OX=3659 GN=Csa_5G167190 PE=3 SV=1)

HSP 1 Score: 938.7 bits (2425), Expect = 1.0e-269
Identity = 458/520 (88.08%), Postives = 489/520 (94.04%), Query Frame = 0


Query: 1   MAQKLHFSIILISLFILSSISLCFSTKPNNNAIDLWCSRMPYPDVCKHFF-NGQFDPKKL 60
           MAQKLHFSIIL S+FILSS SL FSTK NN AI+LWCSR PYPDVCKHFF NG+FDP+ L
Sbjct: 1   MAQKLHFSIILFSMFILSSSSLPFSTKTNNKAIELWCSRTPYPDVCKHFFNNGEFDPRNL 60

Query: 61  SEFKKTALKIAMDRAIRAETLTKELGKKCRNEKERAAWADCLELYQTTILHLNKTFSDEK 120
            + KK ALKIAM+RA++ ETLTK LG+KCRN+KERAAWADCLELYQTTILHLNKTFSD+ 
Sbjct: 61  LDIKKAALKIAMERAMKTETLTKALGQKCRNKKERAAWADCLELYQTTILHLNKTFSDKN 120

Query: 121 CSDFDIQTWLSSALTDLETCRAGFIDLKIKDYDVVFPFLANNNVTKLISNSLAMNNCSES 180
           CS+FDIQTWLSSALT+L TCRAGF+DL IKDY VVFPFL NNN+TKLISNSLAMNNCSES
Sbjct: 121 CSNFDIQTWLSSALTNLHTCRAGFVDLGIKDYGVVFPFLENNNITKLISNSLAMNNCSES 180

Query: 181 DVGYKSNEGFPKWVRRGDRRLLQAAEPKADLVVAQDGSGNYRTVQAAVDAAGKRKGSGRF 240
           D G  S+EGFPKW+  GDRRLLQAAEPKADLVVAQDGSGNY+TVQAAVDAAGKRKGSGRF
Sbjct: 181 DEGNTSDEGFPKWLHGGDRRLLQAAEPKADLVVAQDGSGNYKTVQAAVDAAGKRKGSGRF 240

Query: 241 VIHIKRGVYKENVVIKVKNLMLVGDGLRYTIITGSRSVGGGSTTFNSATVAVTGERFIAR 300
           VI +K+GVYKENVVIKVKNLMLVGDGL+YTIITGSRSVGGGSTTFNSATVAVTGERFIAR
Sbjct: 241 VIRVKKGVYKENVVIKVKNLMLVGDGLKYTIITGSRSVGGGSTTFNSATVAVTGERFIAR 300

Query: 301 GITFRNTAGPENHQAVALRSGSDLSVFFRCGFEGYQDTLYAHSQRQFYRECYIYGTVDFI 360
           GITFRNTAGP+NHQAVALRSG+DLSVFFRCGFEGYQDTLY HSQRQFYRECYIYGTVDFI
Sbjct: 301 GITFRNTAGPQNHQAVALRSGADLSVFFRCGFEGYQDTLYVHSQRQFYRECYIYGTVDFI 360

Query: 361 FGNAAVVLQNCMIYARKPIPGQQCVVTAQGRTDPNQNTGISIHNSRVMAADDLKPVVGSV 420
           FGN+AVVLQNCMIYARKP+ GQQCVVTAQGRTDPNQNTGISIHNSRVMA DDLKPV+ SV
Sbjct: 361 FGNSAVVLQNCMIYARKPMQGQQCVVTAQGRTDPNQNTGISIHNSRVMATDDLKPVIKSV 420

Query: 421 RTYLGRPWKEYSRTVYLQNFMDNLVNPAGWLEWSGNFALNTLYYGEFKNFGPGSSTAKRV 480
           RTYLGRPWKEYSRTVYLQ+FMD+LVNPAGWLEWSGNFALNTLYYGEFKN GPGSSTA RV
Sbjct: 421 RTYLGRPWKEYSRTVYLQSFMDSLVNPAGWLEWSGNFALNTLYYGEFKNSGPGSSTANRV 480

Query: 481 KWKGYRVITSATEASKFTVGNFIAGRSWLPGTGVPFTAGL 520
           KWKGYRVITSA+EA+KFTVG+FIAG SWLPGTGVPFTAGL
Sbjct: 481 KWKGYRVITSASEAAKFTVGSFIAGNSWLPGTGVPFTAGL 520

[Close]

BLAST of Bhi12G000009 vs. ExPASy TrEMBL
Match: A0A6J1K9P3 (Pectinesterase OS=Cucurbita maxima OX=3661 GN=LOC111491356 PE=3 SV=1)

HSP 1 Score: 872.5 bits (2253), Expect = 9.0e-250
Identity = 435/522 (83.33%), Postives = 469/522 (89.85%), Query Frame = 0


Query: 1   MAQKLHFSIILISLFILSSISLCFSTKPNNNAIDLWCSRMPYPDVCKHFFNGQFDPKKLS 60
           MAQ LHF I++IS  IL SIS  FS     NA DLWCS+ PYPDVCK FF  +F+PK L+
Sbjct: 1   MAQNLHFPILVISFTILLSISPSFSAP---NAKDLWCSQTPYPDVCKSFFKAKFNPKNLA 60

Query: 61  EFKKTALKIAMDRAIRAETLTKELGKKCRNEKERAAWADCLELYQTTILHLNKTFSDEKC 120
           EFKKTA KIAM+RA+ AETLTK   +KCR  KE+ AWADCLELYQ TILHLN+TFSD  C
Sbjct: 61  EFKKTAFKIAMERALTAETLTKRHAQKCRTPKEKGAWADCLELYQDTILHLNRTFSDNYC 120

Query: 121 SDFDIQTWLSSALTDLETCRAGFIDLKIKDYDVVFPFLA-NNNVTKLISNSLAMNNCSES 180
           SDFDIQTWLSSALT+LETCRAGF+DL IKDYDVVFPFL+ +NNVTKLI NSLAMNNCSE+
Sbjct: 121 SDFDIQTWLSSALTNLETCRAGFVDLNIKDYDVVFPFLSRSNNVTKLIRNSLAMNNCSET 180

Query: 181 DVGYKSNEGFPKWVRRGDRRLLQAAEPKADLVVAQDGSGNYRTVQAAVDAAGKRKGSGRF 240
           + GY  + GFP WV+ G+RRLLQAA PKADLVVA DGSGNY+TVQAAVDAA KRKGSGRF
Sbjct: 181 ETGY--HGGFPTWVKAGERRLLQAAVPKADLVVALDGSGNYKTVQAAVDAAAKRKGSGRF 240

Query: 241 VIHIKRGVYKENVVI--KVKNLMLVGDGLRYTIITGSRSVGGGSTTFNSATVAVTGERFI 300
           VIH+K+GVYKENVVI  KVKNLMLVGDGL+YTIITGSRSVGGGSTTFNSATVAVTGE FI
Sbjct: 241 VIHVKKGVYKENVVIGNKVKNLMLVGDGLKYTIITGSRSVGGGSTTFNSATVAVTGEGFI 300

Query: 301 ARGITFRNTAGPENHQAVALRSGSDLSVFFRCGFEGYQDTLYAHSQRQFYRECYIYGTVD 360
           ARGITFRNTAGPENHQAVALRSGSDLSVF+RC FEGYQDTLY HSQRQFYRECYIYGTVD
Sbjct: 301 ARGITFRNTAGPENHQAVALRSGSDLSVFYRCSFEGYQDTLYVHSQRQFYRECYIYGTVD 360

Query: 361 FIFGNAAVVLQNCMIYARKPIPGQQCVVTAQGRTDPNQNTGISIHNSRVMAADDLKPVVG 420
           FIFGNAAVVLQNCMIYARKP+ GQQCVVTAQGR+DPNQNTGISIHNSRVMA  DL+PV+ 
Sbjct: 361 FIFGNAAVVLQNCMIYARKPMSGQQCVVTAQGRSDPNQNTGISIHNSRVMATADLQPVLS 420

Query: 421 SVRTYLGRPWKEYSRTVYLQNFMDNLVNPAGWLEWSGNFALNTLYYGEFKNFGPGSSTAK 480
           SVRTYLGRPW+EYSRTVYLQ FMD+LVNPAGWLEWSG+FALNTLYYGEFKNFGPGSSTAK
Sbjct: 421 SVRTYLGRPWREYSRTVYLQCFMDSLVNPAGWLEWSGDFALNTLYYGEFKNFGPGSSTAK 480

Query: 481 RVKWKGYRVITSATEASKFTVGNFIAGRSWLPGTGVPFTAGL 520
           RVKWKGYRVITSA EASKFTVG+FIAG+SWLP TGVPFTAGL
Sbjct: 481 RVKWKGYRVITSAAEASKFTVGSFIAGKSWLPATGVPFTAGL 517

[Close]

BLAST of Bhi12G000009 vs. ExPASy TrEMBL
Match: A0A6J1H1Z9 (Pectinesterase OS=Cucurbita moschata OX=3662 GN=LOC111459307 PE=3 SV=1)

HSP 1 Score: 863.6 bits (2230), Expect = 4.2e-247
Identity = 430/522 (82.38%), Postives = 468/522 (89.66%), Query Frame = 0


Query: 1   MAQKLHFSIILISLFILSSISLCFSTKPNNNAIDLWCSRMPYPDVCKHFFNGQFDPKKLS 60
           MAQ  H SI++IS+ IL +IS  FS     NA DLWCS+ PYPDVCK FFN +F+PK L+
Sbjct: 1   MAQNRHVSILVISMSILLAISPSFSAP---NAKDLWCSQTPYPDVCKSFFNAKFNPKNLA 60

Query: 61  EFKKTALKIAMDRAIRAETLTKELGKKCRNEKERAAWADCLELYQTTILHLNKTFSDEKC 120
           EFKKTA KIAM+RA+ AE+LTK   +KCR  KE+ AWADCLELYQ TIL LN+TFSD  C
Sbjct: 61  EFKKTAFKIAMERALTAESLTKRHAQKCRTPKEKGAWADCLELYQDTILQLNRTFSDNYC 120

Query: 121 SDFDIQTWLSSALTDLETCRAGFIDLKIKDYDVVFPFLA-NNNVTKLISNSLAMNNCSES 180
           SDFDIQTWLSSALT+LETCRAGF+DL IKDYDVVFPFL  +NNVTKLI NSLAMNNCSE+
Sbjct: 121 SDFDIQTWLSSALTNLETCRAGFVDLNIKDYDVVFPFLGRSNNVTKLIRNSLAMNNCSET 180

Query: 181 DVGYKSNEGFPKWVRRGDRRLLQAAEPKADLVVAQDGSGNYRTVQAAVDAAGKRKGSGRF 240
           + GY  + GFP WV+ G+RRLLQAA PKADLVVA DGSGNY+TVQAAVDAA KRKGSGRF
Sbjct: 181 ETGY--HGGFPTWVKAGERRLLQAAVPKADLVVALDGSGNYKTVQAAVDAAAKRKGSGRF 240

Query: 241 VIHIKRGVYKENVVI--KVKNLMLVGDGLRYTIITGSRSVGGGSTTFNSATVAVTGERFI 300
           VIH+K+GVYKENVVI  KVKNLMLVGDGL+YTIITGSRSV GGSTTFNSATVAVTGE FI
Sbjct: 241 VIHVKKGVYKENVVIGNKVKNLMLVGDGLKYTIITGSRSVRGGSTTFNSATVAVTGEGFI 300

Query: 301 ARGITFRNTAGPENHQAVALRSGSDLSVFFRCGFEGYQDTLYAHSQRQFYRECYIYGTVD 360
           ARGITFRNTAGP+NHQAVALRSGSDLSVF+RCGFEGYQDTLY HSQRQFY+ECYIYGTVD
Sbjct: 301 ARGITFRNTAGPQNHQAVALRSGSDLSVFYRCGFEGYQDTLYVHSQRQFYKECYIYGTVD 360

Query: 361 FIFGNAAVVLQNCMIYARKPIPGQQCVVTAQGRTDPNQNTGISIHNSRVMAADDLKPVVG 420
           FIFGNAAVVLQNCMIYARKP+ GQQCVVTAQGR+DPNQNTGISIHNSRVMA  DL+PV+ 
Sbjct: 361 FIFGNAAVVLQNCMIYARKPMSGQQCVVTAQGRSDPNQNTGISIHNSRVMATADLQPVLS 420

Query: 421 SVRTYLGRPWKEYSRTVYLQNFMDNLVNPAGWLEWSGNFALNTLYYGEFKNFGPGSSTAK 480
           SVRTYLGRPWKEYSRTVY+Q FMD+LVNPAGWLEWSG+FALNTLYYGEFKNFGPGSSTAK
Sbjct: 421 SVRTYLGRPWKEYSRTVYMQCFMDSLVNPAGWLEWSGDFALNTLYYGEFKNFGPGSSTAK 480

Query: 481 RVKWKGYRVITSATEASKFTVGNFIAGRSWLPGTGVPFTAGL 520
           RVKWKGYRVITSA EASKFTVG+FIAG+SWLP TGVPFTAGL
Sbjct: 481 RVKWKGYRVITSAAEASKFTVGSFIAGKSWLPATGVPFTAGL 517

[Close]

BLAST of Bhi12G000009 vs. ExPASy TrEMBL
Match: A0A5A7ULB7 (Pectinesterase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold231G00120 PE=3 SV=1)

HSP 1 Score: 842.4 bits (2175), Expect = 1.0e-240
Identity = 412/449 (91.76%), Postives = 433/449 (96.44%), Query Frame = 0


Query: 71  MDRAIRAETLTKELGKKCRNEKERAAWADCLELYQTTILHLNKTFSDEKCSDFDIQTWLS 130
           M+RAIR ETLTK LG+KCRN+KERAAWADCLELYQTTILHLNKTFSD  CS+FDIQTWLS
Sbjct: 1   MERAIRTETLTKALGQKCRNKKERAAWADCLELYQTTILHLNKTFSDH-CSNFDIQTWLS 60

Query: 131 SALTDLETCRAGFIDLKIKDYDVVFPFLANNNVTKLISNSLAMNNCSESDVGYKSNEGFP 190
           SALT+L+TCRAGF+DL IKDY VVFPFLANNN+TKLISNSLAMNNCSESD G  S+EGFP
Sbjct: 61  SALTNLDTCRAGFVDLGIKDYGVVFPFLANNNITKLISNSLAMNNCSESDEGNTSDEGFP 120

Query: 191 KWVRRGDRRLLQAAEPKADLVVAQDGSGNYRTVQAAVDAAGKRKGSGRFVIHIKRGVYKE 250
           KWVR GDRRLLQAAEPKADLVVAQDGSGNY+TVQAAVDAAGKRKGSGRFVI IK+GVYKE
Sbjct: 121 KWVRGGDRRLLQAAEPKADLVVAQDGSGNYKTVQAAVDAAGKRKGSGRFVIRIKKGVYKE 180

Query: 251 NVVIKVKNLMLVGDGLRYTIITGSRSVGGGSTTFNSATVAVTGERFIARGITFRNTAGPE 310
           NVVIKVKNLMLVGDGLRYTIITGSRSVGGGSTTFNSATVAVTGERFIARGITFRNTAGP+
Sbjct: 181 NVVIKVKNLMLVGDGLRYTIITGSRSVGGGSTTFNSATVAVTGERFIARGITFRNTAGPQ 240

Query: 311 NHQAVALRSGSDLSVFFRCGFEGYQDTLYAHSQRQFYRECYIYGTVDFIFGNAAVVLQNC 370
           NHQAVALRSG+DLSVFFRCGFEGYQDTLYAHSQRQFYRECYIYGT+DFIFGNAAVVLQNC
Sbjct: 241 NHQAVALRSGADLSVFFRCGFEGYQDTLYAHSQRQFYRECYIYGTIDFIFGNAAVVLQNC 300

Query: 371 MIYARKPIPGQQCVVTAQGRTDPNQNTGISIHNSRVMAADDLKPVVGSVRTYLGRPWKEY 430
           MIYARKP+ GQQCVVTAQGRTDPNQNTGISIHNSRVMAADDLKPV+ SVRTYLGRPWKEY
Sbjct: 301 MIYARKPMQGQQCVVTAQGRTDPNQNTGISIHNSRVMAADDLKPVIKSVRTYLGRPWKEY 360

Query: 431 SRTVYLQNFMDNLVNPAGWLEWSGNFALNTLYYGEFKNFGPGSSTAKRVKWKGYRVITSA 490
           SRTVYLQ+FM++LVNPAGWLEWSGNFALNTLYYGEFKN+GPGSSTA RVKWKGYRVITSA
Sbjct: 361 SRTVYLQSFMESLVNPAGWLEWSGNFALNTLYYGEFKNYGPGSSTANRVKWKGYRVITSA 420

Query: 491 TEASKFTVGNFIAGRSWLPGTGVPFTAGL 520
            EA+KFTVGNFIAG SWLPGTGVPFTAGL
Sbjct: 421 NEAAKFTVGNFIAGNSWLPGTGVPFTAGL 448

The following BLAST results are available for this feature:

BLAST of Bhi12G000009 vs. TAIR 10


Match Name	E-value	Identity	Description
AT2G45220.1	9.1e-170	57.83	Plant invertase/pectin methylesterase inhibitor superfamily	[more]
AT5G51490.1	8.8e-165	55.62	Plant invertase/pectin methylesterase inhibitor superfamily	[more]
AT5G51500.1	8.5e-160	53.35	Plant invertase/pectin methylesterase inhibitor superfamily	[more]
AT3G47400.1	4.2e-151	52.35	Plant invertase/pectin methylesterase inhibitor superfamily	[more]
AT1G23200.1	1.2e-124	47.54	Plant invertase/pectin methylesterase inhibitor superfamily	[more]

BLAST of Bhi12G000009 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
O04887	2.5e-180	62.14	Pectinesterase 2 OS=Citrus sinensis OX=2711 GN=PECS-2.1 PE=2 SV=1	[more]
O22149	1.3e-168	57.83	Probable pectinesterase/pectinesterase inhibitor 17 OS=Arabidopsis thaliana OX=3...	[more]
Q9FHN5	1.2e-163	55.62	Probable pectinesterase/pectinesterase inhibitor 59 OS=Arabidopsis thaliana OX=3...	[more]
Q9FHN4	1.2e-158	53.35	Probable pectinesterase/pectinesterase inhibitor 60 OS=Arabidopsis thaliana OX=3...	[more]
Q9STY3	6.0e-150	52.35	Probable pectinesterase/pectinesterase inhibitor 33 OS=Arabidopsis thaliana OX=3...	[more]

BLAST of Bhi12G000009 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_038875884.1	3.4e-304	100.00	pectinesterase 2-like [Benincasa hispida]	[more]
XP_008436965.1	2.7e-272	90.00	PREDICTED: pectinesterase 2-like [Cucumis melo]	[more]
XP_004143735.1	2.1e-269	88.08	pectinesterase 2 [Cucumis sativus] >KGN50327.1 hypothetical protein Csa_000416 [...	[more]
XP_022996028.1	1.9e-249	83.33	pectinesterase-like [Cucurbita maxima]	[more]
XP_023534348.1	5.1e-247	82.38	pectinesterase-like [Cucurbita pepo subsp. pepo]	[more]

BLAST of Bhi12G000009 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A1S3ASJ4	1.3e-272	90.00	Pectinesterase OS=Cucumis melo OX=3656 GN=LOC103482536 PE=3 SV=1	[more]
A0A0A0KPG9	1.0e-269	88.08	Pectinesterase OS=Cucumis sativus OX=3659 GN=Csa_5G167190 PE=3 SV=1	[more]
A0A6J1K9P3	9.0e-250	83.33	Pectinesterase OS=Cucurbita maxima OX=3661 GN=LOC111491356 PE=3 SV=1	[more]
A0A6J1H1Z9	4.2e-247	82.38	Pectinesterase OS=Cucurbita moschata OX=3662 GN=LOC111459307 PE=3 SV=1	[more]
A0A5A7ULB7	1.0e-240	91.76	Pectinesterase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold231G00120...	[more]

InterPro

Analysis Name: InterPro Annotations of Wax gourd (B227) v1
Date Performed: 2021-10-22


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR006501	Pectinesterase inhibitor domain	SMART	SM00856	PMEI_2	coord: 28..173 e-value: 6.9E-30 score: 115.3
IPR006501	Pectinesterase inhibitor domain	TIGRFAM	TIGR01614	TIGR01614	coord: 8..175 e-value: 1.9E-22 score: 77.5
IPR006501	Pectinesterase inhibitor domain	PFAM	PF04043	PMEI	coord: 32..173 e-value: 1.7E-25 score: 90.1
IPR000070	Pectinesterase, catalytic	PFAM	PF01095	Pectinesterase	coord: 209..504 e-value: 2.9E-142 score: 472.7
IPR012334	Pectin lyase fold	GENE3D	2.160.20.10		coord: 203..519 e-value: 1.1E-124 score: 418.0
IPR035513	Invertase/pectin methylesterase inhibitor domain superfamily	GENE3D	1.20.140.40	Invertase/pectin methylesterase inhibitor family protein	coord: 28..176 e-value: 2.3E-27 score: 97.9
IPR035513	Invertase/pectin methylesterase inhibitor domain superfamily	SUPERFAMILY	101148	Plant invertase/pectin methylesterase inhibitor	coord: 30..176
None	No IPR available	PANTHER	PTHR31707:SF181	PECTINESTERASE	coord: 8..518
None	No IPR available	PANTHER	PTHR31707	PECTINESTERASE	coord: 8..518
None	No IPR available	CDD	cd15798	PMEI-like_3	coord: 35..175 e-value: 3.12971E-32 score: 119.08
IPR018040	Pectinesterase, Tyr active site	PROSITE	PS00800	PECTINESTERASE_1	coord: 235..254
IPR033131	Pectinesterase, Asp active site	PROSITE	PS00503	PECTINESTERASE_2	coord: 352..361
IPR011050	Pectin lyase fold/virulence factor	SUPERFAMILY	51126	Pectin lyase-like	coord: 206..518

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Bhi12M000009	Bhi12M000009	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0042545	cell wall modification
biological_process	GO:0043086	negative regulation of catalytic activity
biological_process	GO:0045490	pectin catabolic process
cellular_component	GO:0005576	extracellular region
cellular_component	GO:0016021	integral component of membrane
molecular_function	GO:0045330	aspartyl esterase activity
molecular_function	GO:0030599	pectinesterase activity
molecular_function	GO:0046910	pectinesterase inhibitor activity
molecular_function	GO:0004857	enzyme inhibitor activity