Bhi09G001165 (gene) Wax gourd (B227) v1

Overview


Name	Bhi09G001165
Type	gene
Organism	Benincasa hispida (Wax gourd (B227) v1)
Description	beta-amyrin 11-oxidase-like
Location	chr9: 35534714 .. 35534888 (+)
RNA-Seq Expression	Bhi09G001165
Synteny	Bhi09G001165

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

GGCTTTATGTGGTTTTGTTTGTGTTCCTAAGGAGATTCAACGAAGTATGGAGTTTTCTAAAATTGGGAAGGAAGTTTACAGAGCTCTTCCTCCGGGCGATATGGGATGGCCCCTTATCGGCTCTTCCTTGTTGTTTACCAATTCTTCTAAGACAAATCATGACCCTGATTCTTTC

mRNA sequence

GGCTTTATGTGGTTTTGTTTGTGTTCCTAAGGAGATTCAACGAAGTATGGAGTTTTCTAAAATTGGGAAGGAAGTTTACAGAGCTCTTCCTCCGGGCGATATGGGATGGCCCCTTATCGGCTCTTCCTTGTTGTTTACCAATTCTTCTAAGACAAATCATGACCCTGATTCTTTC

Coding sequence (CDS)

GGCTTTATGTGGTTTTGTTTGTGTTCCTAAGGAGATTCAACGAAGTATGGAGTTTTCTAAAATTGGGAAGGAAGTTTACAGAGCTCTTCCTCCGGGCGATATGGGATGGCCCCTTATCGGCTCTTCCTTGTTGTTTACCAATTCTTCTAAGACAAATCATGACCCTGATTCTTTC

Protein sequence

ALCGFVCVPKEIQRSMEFSKIGKEVYRALPPGDMGWPLIGSSLLFTNSSKTNHDPDSF

Homology

[Close]

BLAST of Bhi09G001165 vs. TAIR 10
Match: AT2G32440.1 (ent-kaurenoic acid hydroxylase 2 )

HSP 1 Score: 44.3 bits (103), Expect = 3.9e-05
Identity = 22/51 (43.14%), Postives = 34/51 (66.67%), Query Frame = 0


Query: 8  VPKEIQRSMEFSKIGKEVYRALPPGDMGWPLIGSSLLFTNSSKTNHDPDSF 59
          V K +   +  SK+G++ +  LPPGD+GWP+IG+   F  + KT+ DP+SF
Sbjct: 24 VLKRVNVWIYVSKLGEKKH-YLPPGDLGWPVIGNMWSFLRAFKTS-DPESF 72

[Close]

BLAST of Bhi09G001165 vs. TAIR 10
Match: AT2G32440.2 (ent-kaurenoic acid hydroxylase 2 )

HSP 1 Score: 44.3 bits (103), Expect = 3.9e-05
Identity = 22/51 (43.14%), Postives = 34/51 (66.67%), Query Frame = 0


Query: 8  VPKEIQRSMEFSKIGKEVYRALPPGDMGWPLIGSSLLFTNSSKTNHDPDSF 59
          V K +   +  SK+G++ +  LPPGD+GWP+IG+   F  + KT+ DP+SF
Sbjct: 24 VLKRVNVWIYVSKLGEKKH-YLPPGDLGWPVIGNMWSFLRAFKTS-DPESF 72

[Close]

BLAST of Bhi09G001165 vs. TAIR 10
Match: AT1G05160.1 (cytochrome P450, family 88, subfamily A, polypeptide 3 )

HSP 1 Score: 43.5 bits (101), Expect = 6.7e-05
Identity = 19/30 (63.33%), Postives = 23/30 (76.67%), Query Frame = 0


Query: 29 LPPGDMGWPLIGSSLLFTNSSKTNHDPDSF 59
          LPPGD+GWP IG+ L F  + KT+ DPDSF
Sbjct: 45 LPPGDLGWPFIGNMLSFLRAFKTS-DPDSF 73

[Close]

BLAST of Bhi09G001165 vs. ExPASy Swiss-Prot
Match: Q5VRM7 (Ent-kaurenoic acid oxidase OS=Oryza sativa subsp. japonica OX=39947 GN=KAO PE=2 SV=1)

HSP 1 Score: 44.7 bits (104), Expect = 4.2e-04
Identity = 21/60 (35.00%), Postives = 36/60 (60.00%), Query Frame = 0


Query: 2  LCGFVCVPKEIQRSMEFSKI---GKEVYRALPPGDMGWPLIGSSLLFTNSSKTNHDPDSF 59
          + G V V   ++R+ ++ ++   G E    LPPG+MGWP++GS   F  + K+  +PD+F
Sbjct: 25 VAGVVLVDAVVRRAHDWVRVAALGAERRSRLPPGEMGWPMVGSMWAFLRAFKSG-NPDAF 83

[Close]

BLAST of Bhi09G001165 vs. ExPASy Swiss-Prot
Match: Q9C5Y2 (Ent-kaurenoic acid oxidase 2 OS=Arabidopsis thaliana OX=3702 GN=KAO2 PE=1 SV=2)

HSP 1 Score: 44.3 bits (103), Expect = 5.5e-04
Identity = 22/51 (43.14%), Postives = 34/51 (66.67%), Query Frame = 0


Query: 8  VPKEIQRSMEFSKIGKEVYRALPPGDMGWPLIGSSLLFTNSSKTNHDPDSF 59
          V K +   +  SK+G++ +  LPPGD+GWP+IG+   F  + KT+ DP+SF
Sbjct: 24 VLKRVNVWIYVSKLGEKKH-YLPPGDLGWPVIGNMWSFLRAFKTS-DPESF 72

[Close]

BLAST of Bhi09G001165 vs. ExPASy Swiss-Prot
Match: O23051 (Ent-kaurenoic acid oxidase 1 OS=Arabidopsis thaliana OX=3702 GN=KAO1 PE=1 SV=1)

HSP 1 Score: 43.5 bits (101), Expect = 9.4e-04
Identity = 19/30 (63.33%), Postives = 23/30 (76.67%), Query Frame = 0


Query: 29 LPPGDMGWPLIGSSLLFTNSSKTNHDPDSF 59
          LPPGD+GWP IG+ L F  + KT+ DPDSF
Sbjct: 45 LPPGDLGWPFIGNMLSFLRAFKTS-DPDSF 73

[Close]

BLAST of Bhi09G001165 vs. ExPASy TrEMBL
Match: A0A6J1L122 (beta-amyrin 11-oxidase-like OS=Cucurbita maxima OX=3661 GN=LOC111498127 PE=4 SV=1)

HSP 1 Score: 65.9 bits (159), Expect = 6.5e-08
Identity = 26/49 (53.06%), Postives = 38/49 (77.55%), Query Frame = 0


Query: 10 KEIQRSMEFSKIGKEVYRALPPGDMGWPLIGSSLLFTNSSKTNHDPDSF 59
          + +     F K+G++VY+ALPPGDMGWPLIGSSL   N+ K+++DP++F
Sbjct: 25 RRLNELQSFVKLGRKVYQALPPGDMGWPLIGSSLSLYNTFKSSNDPNTF 73

[Close]

BLAST of Bhi09G001165 vs. ExPASy TrEMBL
Match: A0A6J1H6Q2 (beta-amyrin 11-oxidase-like OS=Cucurbita moschata OX=3662 GN=LOC111460619 PE=3 SV=1)

HSP 1 Score: 64.3 bits (155), Expect = 1.9e-07
Identity = 26/41 (63.41%), Postives = 36/41 (87.80%), Query Frame = 0


Query: 18 FSKIGKEVYRALPPGDMGWPLIGSSLLFTNSSKTNHDPDSF 59
          F K+G++VY+ALPPGDMGWPLIGSSL   N+ K+++DP++F
Sbjct: 33 FVKLGRKVYQALPPGDMGWPLIGSSLSLYNTFKSSNDPNTF 73

[Close]

BLAST of Bhi09G001165 vs. ExPASy TrEMBL
Match: A0A6J1GJM1 (beta-amyrin 11-oxidase-like OS=Cucurbita moschata OX=3662 GN=LOC111454910 PE=3 SV=1)

HSP 1 Score: 61.2 bits (147), Expect = 1.6e-06
Identity = 25/58 (43.10%), Postives = 36/58 (62.07%), Query Frame = 0


Query: 1  ALCGFVCVPKEIQRSMEFSKIGKEVYRALPPGDMGWPLIGSSLLFTNSSKTNHDPDSF 59
          A+ G     +++       K+G++ Y++LPPGDMGWPL+GSS  F N+     DPDSF
Sbjct: 12 AVLGVYMFVRKLNEIWYLVKLGRKAYKSLPPGDMGWPLLGSSFSFYNAFTVGGDPDSF 69

[Close]

BLAST of Bhi09G001165 vs. ExPASy TrEMBL
Match: A0A1S4E5L3 (beta-amyrin 11-oxidase-like OS=Cucumis melo OX=3656 GN=LOC103503515 PE=3 SV=1)

HSP 1 Score: 60.1 bits (144), Expect = 3.6e-06
Identity = 24/39 (61.54%), Postives = 30/39 (76.92%), Query Frame = 0


Query: 20 KIGKEVYRALPPGDMGWPLIGSSLLFTNSSKTNHDPDSF 59
          K+G+ VY++LPPGD+GWPLIGSS  F  + K   DPDSF
Sbjct: 32 KLGRRVYKSLPPGDLGWPLIGSSFSFYKAFKAGGDPDSF 70

[Close]

BLAST of Bhi09G001165 vs. ExPASy TrEMBL
Match: A0A6J1GLR1 (beta-amyrin 11-oxidase-like OS=Cucurbita moschata OX=3662 GN=LOC111455409 PE=3 SV=1)

HSP 1 Score: 59.3 bits (142), Expect = 6.1e-06
Identity = 23/39 (58.97%), Postives = 30/39 (76.92%), Query Frame = 0


Query: 20 KIGKEVYRALPPGDMGWPLIGSSLLFTNSSKTNHDPDSF 59
          K+G++ Y++LPPGDMGWPL+GSS  F N+     DPDSF
Sbjct: 31 KLGRKAYKSLPPGDMGWPLLGSSFSFYNAFTVGGDPDSF 69

The following BLAST results are available for this feature:

BLAST of Bhi09G001165 vs. TAIR 10


Match Name	E-value	Identity	Description
AT2G32440.1	3.9e-05	43.14	ent-kaurenoic acid hydroxylase 2	[more]
AT2G32440.2	3.9e-05	43.14	ent-kaurenoic acid hydroxylase 2	[more]
AT1G05160.1	6.7e-05	63.33	cytochrome P450, family 88, subfamily A, polypeptide 3	[more]

BLAST of Bhi09G001165 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q5VRM7	4.2e-04	35.00	Ent-kaurenoic acid oxidase OS=Oryza sativa subsp. japonica OX=39947 GN=KAO PE=2 ...	[more]
Q9C5Y2	5.5e-04	43.14	Ent-kaurenoic acid oxidase 2 OS=Arabidopsis thaliana OX=3702 GN=KAO2 PE=1 SV=2	[more]
O23051	9.4e-04	63.33	Ent-kaurenoic acid oxidase 1 OS=Arabidopsis thaliana OX=3702 GN=KAO1 PE=1 SV=1	[more]

BLAST of Bhi09G001165 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1L122	6.5e-08	53.06	beta-amyrin 11-oxidase-like OS=Cucurbita maxima OX=3661 GN=LOC111498127 PE=4 SV=...	[more]
A0A6J1H6Q2	1.9e-07	63.41	beta-amyrin 11-oxidase-like OS=Cucurbita moschata OX=3662 GN=LOC111460619 PE=3 S...	[more]
A0A6J1GJM1	1.6e-06	43.10	beta-amyrin 11-oxidase-like OS=Cucurbita moschata OX=3662 GN=LOC111454910 PE=3 S...	[more]
A0A1S4E5L3	3.6e-06	61.54	beta-amyrin 11-oxidase-like OS=Cucumis melo OX=3656 GN=LOC103503515 PE=3 SV=1	[more]
A0A6J1GLR1	6.1e-06	58.97	beta-amyrin 11-oxidase-like OS=Cucurbita moschata OX=3662 GN=LOC111455409 PE=3 S...	[more]

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Bhi09M001165	Bhi09M001165	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
cellular_component	GO:0016021	integral component of membrane
molecular_function	GO:0020037	heme binding
molecular_function	GO:0005506	iron ion binding
molecular_function	GO:0004497	monooxygenase activity
molecular_function	GO:0016705	oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen