Bhi09G000065 (gene) Wax gourd (B227) v1

Overview
NameBhi09G000065
Typegene
OrganismBenincasa hispida (Wax gourd (B227) v1)
Description2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein
Locationchr9: 1534911 .. 1538097 (+)
RNA-Seq ExpressionBhi09G000065
SyntenyBhi09G000065
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRexonCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
GTTTTAAAAACTTGTTTAAATTCTCTTACAAATAAGCCATCAAAACAAAATGAAAATTGGTAGCATTGAGTAAAGCTAAGGTCTTGTAGGACAGAATGGTTAATTCTGAAGCAAAAGCCAAAGTACTTCCCACTATAGATTTGTCAAAATTAGAGGCAGGTGGCACAGAAGAATGGGGTTTAGCATGCAAAGAGATGAGGCTTGGATTAGAAGAATTTGGTTGTTTCTCAGTGGTTTATGACAGAGTTTCTTTGGAGCTTCATAACTCCATTTTTGAAGCAACAAAAGAGCTTTTTCATCTCCCATTAGAAACTAGAATTAAGAACACTAGTGAAAAGCCTTACCATGGCTATTTTGGTGGATACTCTGTTCTTCCTCTCTATGAATCCATGGGTATTGACAATCCAATTGAGCTACAAGCAACTCAGAGTTTCGCTAATCTCATGTGGCCTACTAAAGAAAATAATCACTTCTGGTAATTCATTAAACACACACTTGACTTCTCTCGTCAACTATTTAGTTTCAATTTTATTCTTATGAATTCCGAAATGTCATTGAACTTCTATTTGTTTTTTGGTTCCATATAGATCATTGAAGTTTATAAAAATTTTAAAAGTTAACCAATCCATTAGATATAGAATTTAATTTAATCGCTTCAAAAAACCACTTTTAGCCCAACATAGGATGGAAGTAACAATTTAATCCATGAACTTTTTGATTTGTAACGATTTAACCCCTTTACATTCAATTCTGAATTTTAGTGCTTAACGATTTGTTCCACTACACTTTCAAATTTGTAACAATTTAATATCTATATAAAAAAATAAATGATGACTTGTTTTATTATTATTTAAAGGATGTAGACTTTTTAGCCTTATAAGTATATTGGTGTCATGCCTAATTAGTTAATTGATTGATCTACCTAATGCAAGAAATCTTATTAAATATTAACACAAATTTTTATAATAAACACTAAATAGTTATACATTTTAAAGTGTAAGAACTAAATTGTTACATATATAGTTCAAAGACTAAACTGTTGTAAACCAAAGTCTAGAGATAAAATTATTATTTCCATTGAAGTTTAGAGACCAAAAATTATATATATATATATGCAAAGGTATTGTAATTAATGTGTGGGGTGGGAGAATCGAACCTCAGACCTCAATTGATACCAATTTTATATTTAATAGGATCCTAAAACTTCAATTTAATGCCTATTAATTCCATGAATCTATATGTATGTATTGCTTTTGAATTCAGTGCATGATAAGAATGCAATGGATGTGTCAACCTAATTGAGATATCTGAATCCCTAGTGTACCTCATGATCCCTCTCTCCTATATTCACTTATTAAAAAAACAAAGTAGCTCCATGAATTTGTGAACACTGAGATTAATGATTTGACAGTGGAACTGTGGAACGTTTCTCAACGTTGGTAGCGGAATTGGAGAAGATGGTAACAAAAATGGTATTGGAAAGCTATGGACTATCGAAGCTTGTAAATGAGCCAAACATGGAGTCAGCCAATTACCTTCTTCGATGCTTCAAATACAGGGCGCCTGAGAAAGATGAAACCGATGTTGGTTTGCATTCCCATACAGATCTCACTTTCTTTTCCATAATTCATCAGCATCTCATTTCTGGTCTTCAAGTTCAATCACTTGATGATCAATGGATTGATATTCAACCCTCAGGTCATTGCTATTTTATTGTCATGGCTGGCGATGCACTCATGGTAATCCTAATTAGCCTCAATCCTTTTCTGTTTTAAGTCCTAATCGGATCATAAAAGAAATAAGTTTGTCTCGTGAAACCATGTGATTCTTAAAACATATAAATGTGAAAATATAAAAAATATCATTGAACTATATAATTGTTGGTTTCAACTATACTTTTAACTTTTAAGAATTTCATTTTGATCCTTATTAAAGTTTGAAGTTTATTTCAAAACAACTCTTGTTAAGCTTTATTTTACTGTTAAGTTCATACACATAAAAGTTTATACTTGTTTATGTGAACAAATCATATAAATACGACAAATAAAGACACATGCATGACACATTCGTGAAACATATATTATTATTTATTATCGTCTTCCCTCCTTTCAACCCTACATGATTGTTTGTTATTTTTGCTAGCCATGAATTTAACAAGAAAAGCCTTCAACAGTTATTTTCTAAGAAAAAAACAACTTCAAATATTCAAGGGTTAAAATGAAATTTTTATAAGTTCATGAGTATAATTGAAACCAGTCCCATACTTCAGGAGTATTTTTGATAATTTAACATTTCTAAAACGTAAATGTGAACTTTAAAACGTAAGAACTAAACTTATAATTGAACCGTTGATTGTATTATTATTGATGGGGTGTAGGCATGGAGCAACGAGAGAATAAGGTCTTGTAGGCACCGAGTGATAATGAGAGGAGAGGAAACAAGATACTCAATTGGGATGTTTTCATTCAAGAATGGGACGATTGCTGTGCCTGAAGAGCTTGTGGATGATGCCAATCCACTACGATACAAGCCATTTAATCACTATGATTTCCTTACCTATGACAAAGCCAAGGCTTCTCATCAAAATGTCTCTCGTATCAAAGATTATTGTGGCATTTGAATCAACAAAGCATCCATCATCAACAATCCTATATTTCACCTTTCCATAATCGTCAAATTTGTTGTTATAGGCTCAAAATTTTGAGTGGGATTCACTCATCTATTAAGCCTACAATAAAGGTTATGTTTCCCTTTCTTTCTTCTATAACTTCAATCGTATGTGAATTACTATTCATATAATTTGACTTTGATTAAAAAATAAGCATGTTCTTTGATAATCATTAGATAAAGTCTTTTTGCTCTTTCTCTCTTTACATTTTGGGTAATTCTTGGCCTTCAATAGAGTATCCATTAGTGTTCTTCCTCACATTGTGTAGCTTTTGTCACACTTTTGATTTCATATTCTTCAACCAATTCCAGAGATCCATATCCGATTTGTGTTTTTAAGTTTTCTTCCAATTCCTTTCCTTTCTCTTCAGTGGAGAGGAAACCAACTTTTTCTCTCTTCTAAAAGAGGACACCATTCATGGGCTTTCAGATTTTTCAATTATTTGATGTCGGTTTTCAACCGACATTGAACGTCAACTTTAATGTCGGGTTTTACTTATTTTTTATTCTCAAGTATCCG

mRNA sequence

GTTTTAAAAACTTGTTTAAATTCTCTTACAAATAAGCCATCAAAACAAAATGAAAATTGGTAGCATTGAGTAAAGCTAAGGTCTTGTAGGACAGAATGGTTAATTCTGAAGCAAAAGCCAAAGTACTTCCCACTATAGATTTGTCAAAATTAGAGGCAGGTGGCACAGAAGAATGGGGTTTAGCATGCAAAGAGATGAGGCTTGGATTAGAAGAATTTGGTTGTTTCTCAGTGGTTTATGACAGAGTTTCTTTGGAGCTTCATAACTCCATTTTTGAAGCAACAAAAGAGCTTTTTCATCTCCCATTAGAAACTAGAATTAAGAACACTAGTGAAAAGCCTTACCATGGCTATTTTGGTGGATACTCTGTTCTTCCTCTCTATGAATCCATGGGTATTGACAATCCAATTGAGCTACAAGCAACTCAGAGTTTCGCTAATCTCATGTGGCCTACTAAAGAAAATAATCACTTCTGTGGAACTGTGGAACGTTTCTCAACGTTGGTAGCGGAATTGGAGAAGATGGTAACAAAAATGGTATTGGAAAGCTATGGACTATCGAAGCTTGTAAATGAGCCAAACATGGAGTCAGCCAATTACCTTCTTCGATGCTTCAAATACAGGGCGCCTGAGAAAGATGAAACCGATGTTGGTTTGCATTCCCATACAGATCTCACTTTCTTTTCCATAATTCATCAGCATCTCATTTCTGGTCTTCAAGTTCAATCACTTGATGATCAATGGATTGATATTCAACCCTCAGGTCATTGCTATTTTATTGTCATGGCTGGCGATGCACTCATGGCATGGAGCAACGAGAGAATAAGGTCTTGTAGGCACCGAGTGATAATGAGAGGAGAGGAAACAAGATACTCAATTGGGATGTTTTCATTCAAGAATGGGACGATTGCTGTGCCTGAAGAGCTTGTGGATGATGCCAATCCACTACGATACAAGCCATTTAATCACTATGATTTCCTTACCTATGACAAAGCCAAGGCTTCTCATCAAAATGTCTCTCGTATCAAAGATTATTGTGGCATTTGAATCAACAAAGCATCCATCATCAACAATCCTATATTTCACCTTTCCATAATCGTCAAATTTGTTGTTATAGGCTCAAAATTTTGAGTGGGATTCACTCATCTATTAAGCCTACAATAAAGGTTATGTTTCCCTTTCTTTCTTCTATAACTTCAATCGTATGTGAATTACTATTCATATAATTTGACTTTGATTAAAAAATAAGCATGTTCTTTGATAATCATTAGATAAAGTCTTTTTGCTCTTTCTCTCTTTACATTTTGGGTAATTCTTGGCCTTCAATAGAGTATCCATTAGTGTTCTTCCTCACATTGTGTAGCTTTTGTCACACTTTTGATTTCATATTCTTCAACCAATTCCAGAGATCCATATCCGATTTGTGTTTTTAAGTTTTCTTCCAATTCCTTTCCTTTCTCTTCAGTGGAGAGGAAACCAACTTTTTCTCTCTTCTAAAAGAGGACACCATTCATGGGCTTTCAGATTTTTCAATTATTTGATGTCGGTTTTCAACCGACATTGAACGTCAACTTTAATGTCGGGTTTTACTTATTTTTTATTCTCAAGTATCCG

Coding sequence (CDS)

ATGGTTAATTCTGAAGCAAAAGCCAAAGTACTTCCCACTATAGATTTGTCAAAATTAGAGGCAGGTGGCACAGAAGAATGGGGTTTAGCATGCAAAGAGATGAGGCTTGGATTAGAAGAATTTGGTTGTTTCTCAGTGGTTTATGACAGAGTTTCTTTGGAGCTTCATAACTCCATTTTTGAAGCAACAAAAGAGCTTTTTCATCTCCCATTAGAAACTAGAATTAAGAACACTAGTGAAAAGCCTTACCATGGCTATTTTGGTGGATACTCTGTTCTTCCTCTCTATGAATCCATGGGTATTGACAATCCAATTGAGCTACAAGCAACTCAGAGTTTCGCTAATCTCATGTGGCCTACTAAAGAAAATAATCACTTCTGTGGAACTGTGGAACGTTTCTCAACGTTGGTAGCGGAATTGGAGAAGATGGTAACAAAAATGGTATTGGAAAGCTATGGACTATCGAAGCTTGTAAATGAGCCAAACATGGAGTCAGCCAATTACCTTCTTCGATGCTTCAAATACAGGGCGCCTGAGAAAGATGAAACCGATGTTGGTTTGCATTCCCATACAGATCTCACTTTCTTTTCCATAATTCATCAGCATCTCATTTCTGGTCTTCAAGTTCAATCACTTGATGATCAATGGATTGATATTCAACCCTCAGGTCATTGCTATTTTATTGTCATGGCTGGCGATGCACTCATGGCATGGAGCAACGAGAGAATAAGGTCTTGTAGGCACCGAGTGATAATGAGAGGAGAGGAAACAAGATACTCAATTGGGATGTTTTCATTCAAGAATGGGACGATTGCTGTGCCTGAAGAGCTTGTGGATGATGCCAATCCACTACGATACAAGCCATTTAATCACTATGATTTCCTTACCTATGACAAAGCCAAGGCTTCTCATCAAAATGTCTCTCGTATCAAAGATTATTGTGGCATTTGA

Protein sequence

MVNSEAKAKVLPTIDLSKLEAGGTEEWGLACKEMRLGLEEFGCFSVVYDRVSLELHNSIFEATKELFHLPLETRIKNTSEKPYHGYFGGYSVLPLYESMGIDNPIELQATQSFANLMWPTKENNHFCGTVERFSTLVAELEKMVTKMVLESYGLSKLVNEPNMESANYLLRCFKYRAPEKDETDVGLHSHTDLTFFSIIHQHLISGLQVQSLDDQWIDIQPSGHCYFIVMAGDALMAWSNERIRSCRHRVIMRGEETRYSIGMFSFKNGTIAVPEELVDDANPLRYKPFNHYDFLTYDKAKASHQNVSRIKDYCGI
Homology
BLAST of Bhi09G000065 vs. TAIR 10
Match: AT1G52800.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 288.9 bits (738), Expect = 5.0e-78
Identity = 141/312 (45.19%), Postives = 198/312 (63.46%), Query Frame = 0

Query: 7   KAKVLPTIDLSKLE--AGGTEEWGLACKEMRLGLEEFGCFSVVYDRVSLELHNSIFEATK 66
           K   +P +DL+  +     T  W    +E    LEE+GCF  VYD V+ +L +SIF A +
Sbjct: 6   KTTKVPILDLTSQQELKPNTSTWRSVSREACEALEEYGCFLAVYDGVTQQLDDSIFAAAE 65

Query: 67  ELFHLPLETRIKNTSEKPYHGYFGGYSVLPLYESMGIDNPIELQATQSFANLMWPTKENN 126
           ELF LP ET+ KN +EKPYHGY G   V+PL+E +G+D     +  Q F +LMWP + N+
Sbjct: 66  ELFDLPTETKKKNVNEKPYHGYVGQMPVIPLHEGLGVDYVTNKEIAQRFTHLMWP-QGND 125

Query: 127 HFCGTVERFSTLVAELEKMVTKMVLESYGLSKLVNEPNMESANYLLRCFKYRAPEKDETD 186
            FC TV  FS  VAEL+++V +M+ E+YG+ K   E ++ S  YLL+  KY AP +  + 
Sbjct: 126 RFCNTVHTFSNAVAELDRLVVRMIFENYGVEKHY-ESHVGSKTYLLKFLKYLAPPESISM 185

Query: 187 VGLHSHTDLTFFSIIHQHLISGLQVQSLDDQWIDIQPSGHCYFIVMAGDALMAWSNERIR 246
                HTD TF SI+HQ+ ++GL+V+S D +WI +Q     Y +VMAGD  M WSN+RIR
Sbjct: 186 PAFPQHTDKTFLSILHQNDVNGLEVKSKDGEWISLQLPPKSY-VVMAGDISMGWSNDRIR 245

Query: 247 SCRHRVIMRGEETRYSIGMFSFKNGTIAVPEELVDDANPLRYKPFNHYDFLTYDKAKASH 306
           SC HRV M G++TRY++G+FSF    +++PEELVDD +PL YKPF++   + +   K   
Sbjct: 246 SCEHRVTMEGDKTRYTLGLFSFLTDLVSIPEELVDDKHPLMYKPFDNIALINFYTTKEGR 305

Query: 307 QNVSRIKDYCGI 317
           +  S +K YCGI
Sbjct: 306 EANSTLKAYCGI 314

BLAST of Bhi09G000065 vs. TAIR 10
Match: AT1G52790.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 279.3 bits (713), Expect = 3.9e-75
Identity = 134/307 (43.65%), Postives = 204/307 (66.45%), Query Frame = 0

Query: 11  LPTIDLSKLE-AGGTEEWGLACKEMRLGLEEFGCFSV-VYDRVSLELHNSIFEATKELFH 70
           +PT+D S+ +   GT+ W    + +R  LEE+GCF + + D+  L+L + +F +  +LF 
Sbjct: 5   IPTLDFSREDLKPGTKYWESTRENIRQALEEYGCFIIDLKDKTPLDLLDRVFGSLVDLFD 64

Query: 71  LPLETRIKNTSEKPYHGYFGGYSVLPLYESMGIDNPIELQATQSFANLMWPTKENNHFCG 130
           LP +T++KN  +KP +GY G    LPL+ES+GIDN   L+AT+SF  LMWP + N HF  
Sbjct: 65  LPTQTKMKNKYDKPLNGYVGQIPALPLHESLGIDNATSLEATRSFTGLMWP-QGNEHFSE 124

Query: 131 TVERFSTLVAELEKMVTKMVLESYGLSKLVNEPNMESANYLLRCFKYRAPEKDETDVGLH 190
            + +++   AEL++MVT+MV +SY + K   +P +ES  YLLR  K RAP  +   +G  
Sbjct: 125 CLYKYAEFAAELDQMVTRMVFQSYNVEKYY-DPYIESTTYLLRVLKNRAPNNENPTLGFV 184

Query: 191 SHTDLTFFSIIHQHLISGLQVQSLDDQWIDIQPSGHCYFIVMAGDALMAWSNERIRSCRH 250
           +HTD +F +I+HQ  ++GL++++ + + I+I  S    F+V+AGDALMAWSN+R+ S RH
Sbjct: 185 THTDKSFTTILHQDQVNGLEMETREGERININLSSPSLFMVVAGDALMAWSNDRVWSPRH 244

Query: 251 RVIMRGEETRYSIGMFSFKNGTIAVPEELVDDANPLRYKPFNHYDFLTYDKAKASHQNVS 310
           +V++ GE  RYS+GMF+F NGT+ VPEEL+D  +PL YKPF+H   L + +    +++  
Sbjct: 245 QVLVSGETDRYSLGMFAFNNGTLQVPEELIDHQHPLMYKPFDHLGLLRFYRTDTGYKSEC 304

Query: 311 RIKDYCG 316
            IK YCG
Sbjct: 305 PIKAYCG 309

BLAST of Bhi09G000065 vs. TAIR 10
Match: AT1G52820.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 270.4 bits (690), Expect = 1.8e-72
Identity = 131/309 (42.39%), Postives = 201/309 (65.05%), Query Frame = 0

Query: 11  LPTIDLS-KLEAGGTEEWGLACKEMRLGLEEFGCFSVVYDRVSLELHNSIFEATKELFHL 70
           LP ID S K    G  EW L   +++  L+++G F   +DR+  EL  S+F A +ELF L
Sbjct: 12  LPVIDFSNKNLKPGEPEWDLTRADVQKALQDYGYFEASFDRIPFELRKSVFGALEELFDL 71

Query: 71  PLETRIKNTSEKPYHGYFGGYSVLPLYESMGIDNPIELQATQSFANLMWPTKENNHFCGT 130
           PL+T+++N S+KP+HGY G Y ++PLYESMGID+    +   +F   +WP + N  F  T
Sbjct: 72  PLQTKLRNVSKKPFHGYVGQYPMVPLYESMGIDDSDIAEKVDAFTEKLWP-QGNISFSTT 131

Query: 131 VERFSTLVAELEKMVTKMVLESYGLSKLVNEPNMESANYLLRCFKYRAPEKDETDVGLHS 190
           ++ FS  ++EL+  + +M++ES+GL K ++E ++ S NYLLR  KY+ P+ +ET VGL++
Sbjct: 132 IQSFSKKLSELDITIRRMIMESFGLDKYIDE-HLHSTNYLLRVMKYKGPDTEETKVGLNA 191

Query: 191 HTDLTFFSIIHQHLISGLQVQSLDDQWIDIQPSGHCYFIVMAGDALMAWSNERIRSCRHR 250
           HTD    +I++Q+ + GL+VQ+ D  WI ++P+    F VM GD+L A  N R+ S  HR
Sbjct: 192 HTDKNIVTILYQNHVEGLEVQTKDKNWIKVKPTQDS-FTVMIGDSLYALLNGRLHSPYHR 251

Query: 251 VIMRGEETRYSIGMFSFKNG--TIAVPEELVDDANPLRYKPFNHYDFLTYDKAKASHQNV 310
           V+M G ETRYS+G+FS       ++ P+ELVD+ +P  +KPF+H +FL +   +A  ++ 
Sbjct: 252 VMMTGTETRYSLGLFSIPKAGHIVSSPDELVDEEHPRLFKPFDHVEFLQFYYTEAGQRSQ 311

Query: 311 SRIKDYCGI 317
           S +K YCGI
Sbjct: 312 SALKTYCGI 317

BLAST of Bhi09G000065 vs. TAIR 10
Match: AT1G80320.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 238.4 bits (607), Expect = 7.7e-63
Identity = 124/314 (39.49%), Postives = 187/314 (59.55%), Query Frame = 0

Query: 11  LPTIDLSKLE-AGGTEEWGLACKEMRLGLEEFGCFSVVYDRVSLELHNSIFEATKELFHL 70
           LP +D S  +   GT  W     ++R  +E  G F   +  VS +L +++    KE+++L
Sbjct: 9   LPILDFSSDKLVRGTSHWITTRDDVRRAMEGQGWFVAEFSGVSSDLRDNLLAGMKEMYYL 68

Query: 71  PLETRIKNTSEKPYHGYFG-GYSVLPLYESMGIDNPIELQATQSFANLMWPTKENNHFCG 130
           P + +IKN + K  HGY         ++ES+GID   ELQA + F+ L+WP + N+ FC 
Sbjct: 69  PDQIKIKNENHKASHGYMSMVVDDYRIHESLGIDYATELQACKDFSKLLWP-QGNDPFCQ 128

Query: 131 TVERFSTLVAELEKMVTKMVLESYGL-SKLVNEPNMESANYLLRCFKYRAPEKDETDVGL 190
           T   ++  +AEL++ V +M+ ESYG+  K  +  + ES  YLLR   YR  +  E + G 
Sbjct: 129 TTHMYAMTMAELDQTVMRMLYESYGMDEKKHSVSHSESTRYLLRMLSYRRQQNGEANTGF 188

Query: 191 HSHTDLTFFSIIHQHLISGLQVQSLDDQWIDIQPSGHCYFIVMAGDALMAWSNERIRSCR 250
            SHTD +F SI+HQ+ + GLQ++++  QW+   PS    F+V++G  L AWSN+RI++C 
Sbjct: 189 VSHTDKSFMSILHQNHVGGLQLKTMTGQWVGFNPS-PTRFVVLSGMGLTAWSNDRIKACY 248

Query: 251 HRVIMRGEETRYSIGMFSFKNGTIAVPEELVDDANPLRYKPFNHYDFLTY-----DKAKA 310
           H+V+M  +E RYS+G FSF  GTI  PEELVDD +PLRY PF H   L +     +  K 
Sbjct: 249 HKVVMSADEIRYSLGFFSFHKGTIRTPEELVDDQHPLRYNPFEHDGLLRFYESYINSLKK 308

Query: 311 SHQNVSRIKDYCGI 317
           S +++ +I  YCG+
Sbjct: 309 SSEDLLQI--YCGV 318

BLAST of Bhi09G000065 vs. TAIR 10
Match: AT1G15540.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 237.7 bits (605), Expect = 1.3e-62
Identity = 132/317 (41.64%), Postives = 182/317 (57.41%), Query Frame = 0

Query: 7   KAKVLPTIDLS-KLEAGGTEEWGLACKEMRLGLEEFGCFSVVYDRVSLELHNSIFEATKE 66
           + + LP I+LS K     TE W      +R  +E  G F   Y+     LH SI EA KE
Sbjct: 5   ETRELPIINLSDKNLKQNTELWSFTRDSVREAMEHHGWFVAEYNNFPTGLHQSILEAAKE 64

Query: 67  LFHLPLETRIKNTSEKPYHGYFGGYS-VLPLYESMGIDNPIELQATQSFANLMWPT--KE 126
           L  LP+E ++KN + K  HGY    S   P++E +GID   ++Q  + F+ LMWP    +
Sbjct: 65  LLDLPVEIKLKNENHKAGHGYITMMSDGQPVHEGLGIDQVNDVQQCRGFSRLMWPDDHDD 124

Query: 127 NNHFCGTVERFSTLVAELEKMVTKMVLESYGLSKLVNEPNMESANYLLRCFKYRAPEKDE 186
           N+ FC TV  ++ + AELE++V +M+ ESY + K   E  +    YLLR  KYR     E
Sbjct: 125 NDRFCETVHAYAKMQAELEQLVIRMLFESYNVEK-YTEKYIGGTRYLLRLLKYRRLPNGE 184

Query: 187 TDVGLHSHTDLTFFSIIHQHLISGLQVQS-LDDQWIDIQPSGHCYFIVMAGDALMAWSNE 246
            +    SHTD +F SI+HQ+ I+GL ++S  +D W    PS    F+V+AGDA+MAWSN+
Sbjct: 185 PNRKFISHTDKSFISILHQNHITGLMLKSEKEDVWYHFTPS-PTRFVVIAGDAIMAWSND 244

Query: 247 RIRSCRHRVIMRGEETRYSIGMFSFKNGTIAVPEELVDDANPLRYKPFNHYDFLTYDKAK 306
           RI++C H+V M   E RYS+G FSF+ G I+ PEE+VD  +PL Y PF+H   L Y +  
Sbjct: 245 RIKACYHKVEMESVEMRYSLGFFSFQEGMISTPEEMVDKDHPLAYNPFHHDGLLEYYETL 304

Query: 307 ASHQNVSR--IKDYCGI 317
             H    R   K YCGI
Sbjct: 305 EVHLKAHRTMTKAYCGI 319

BLAST of Bhi09G000065 vs. ExPASy Swiss-Prot
Match: Q945B6 (Probable 2-oxoglutarate-dependent dioxygenase AOP1.2 OS=Arabidopsis thaliana OX=3702 GN=AOP1.2 PE=2 SV=1)

HSP 1 Score: 233.4 bits (594), Expect = 3.5e-60
Identity = 123/300 (41.00%), Postives = 181/300 (60.33%), Query Frame = 0

Query: 11  LPTIDLSKLE-AGGTEEWGLACKEMRLGLEEFGCFSVVYDRVSLELHNSIFEATKELFHL 70
           LP ID S      G+ +W     ++   LE++GCF   +D++S+EL+ S+FEA ++LF L
Sbjct: 14  LPVIDFSDQNLKPGSSKWDEVKADVLKALEDYGCFEAFFDKLSVELNRSVFEAMEDLFEL 73

Query: 71  PLETRIKNTSEKPYHGYFGGYSVLPLYESMGIDNPIELQATQSFANLMWPTKENNHFCGT 130
           P+ T+ +N S KP+HGY        LYES+GID+   L+    F   +WP   N     T
Sbjct: 74  PIPTKQRNVSSKPFHGYL----CHNLYESLGIDDANVLEKVNDFTQQLWPDHGNKSISET 133

Query: 131 VERFSTLVAELEKMVTKMVLESYGLSKLVNEPNMESANYLLRCFKYRAPEKD-----ETD 190
           +  FS  + EL+ MV +M++ES+G+   ++E ++ S  YL R  KY +P  D     ET 
Sbjct: 134 IHLFSEQLVELDLMVRRMIMESFGIENYIDE-HLNSTYYLTRLMKYTSPPDDDDDDEETK 193

Query: 191 VGLHSHTDLTFFSIIHQHLISGLQVQSLDDQWIDIQPSGHCYFIVMAGDALMAWSNERIR 250
           +GL SHTD    +I+HQ+ + GL+V++ DD+WI ++PS     +VM GD+L A  N R+ 
Sbjct: 194 LGLRSHTDKNIITILHQYQVDGLEVKTKDDKWIKVKPSQDS-VLVMVGDSLCALLNGRLH 253

Query: 251 SCRHRVIMRGEETRYSIGMFSF-KNGTIA-VPEELVDDANPLRYKPFNHYDFLTYDKAKA 303
           S  HRVIM G++TRYS G+FS  K G I   PEELVD  +P  +KPF + DFL + + +A
Sbjct: 254 SPYHRVIMTGKKTRYSTGLFSIPKTGVIIDSPEELVDKEHPRIFKPFEYTDFLHFFQTEA 307

BLAST of Bhi09G000065 vs. ExPASy Swiss-Prot
Match: Q9ZTA3 (Probable 2-oxoglutarate-dependent dioxygenase AOP1 OS=Arabidopsis thaliana OX=3702 GN=AOP1 PE=2 SV=1)

HSP 1 Score: 231.5 bits (589), Expect = 1.3e-59
Identity = 122/301 (40.53%), Postives = 181/301 (60.13%), Query Frame = 0

Query: 11  LPTIDLSKLE-AGGTEEWGLACKEMRLGLEEFGCFSVVYDRVSLELHNSIFEATKELFHL 70
           LP ID S      G+ +W     ++   LE++GCF   +D++S+EL+ S+FEA ++LF L
Sbjct: 14  LPVIDFSDQNLKPGSSKWDEVTADVLKALEDYGCFEASFDKLSVELNRSVFEAMEDLFEL 73

Query: 71  PLETRIKNTSEKPYHGYFGGYSVLPLYESMGIDNPIELQATQSFANLMWPTKENNHFCGT 130
           P+ T+ +N S KP+HGY        LYES+GI++   L+    F   +WP   N     T
Sbjct: 74  PIPTKQRNVSSKPFHGYL----CHNLYESLGINDANVLEKVNDFTQQLWPDHGNKSISET 133

Query: 131 VERFSTLVAELEKMVTKMVLESYGLSKLVNEPNMESANYLLRCFKYRAPEKD------ET 190
           +  FS  + EL+ MV +M++ES+G+   ++E ++ S  YL R  KY +P  D      ET
Sbjct: 134 IHLFSEQLVELDLMVRRMIMESFGIENYIDE-HLNSTYYLTRLMKYTSPPDDDDDDDEET 193

Query: 191 DVGLHSHTDLTFFSIIHQHLISGLQVQSLDDQWIDIQPSGHCYFIVMAGDALMAWSNERI 250
            +GL SHTD    +I+HQ+ + GL+V++ DD+WI ++PS     +VM GD+L A  N R+
Sbjct: 194 KLGLRSHTDKNIITILHQYQVDGLEVKTKDDKWIKVKPSQDS-VLVMVGDSLCALLNGRL 253

Query: 251 RSCRHRVIMRGEETRYSIGMFSF-KNGTIA-VPEELVDDANPLRYKPFNHYDFLTYDKAK 303
            S  HRVIM G++TRYS G+FS  K G I   PEELVD  +P  +KPF + DFL + + +
Sbjct: 254 HSPYHRVIMTGKKTRYSTGLFSIPKTGVIIDSPEELVDKEHPRIFKPFEYTDFLHFFQTE 308

BLAST of Bhi09G000065 vs. ExPASy Swiss-Prot
Match: Q944Z5 (Probable 2-oxoglutarate-dependent dioxygenase AOP1 (Fragment) OS=Arabidopsis thaliana OX=3702 GN=AOP1 PE=2 SV=1)

HSP 1 Score: 230.3 bits (586), Expect = 3.0e-59
Identity = 122/299 (40.80%), Postives = 181/299 (60.54%), Query Frame = 0

Query: 11  LPTIDLSKLE-AGGTEEWGLACKEMRLGLEEFGCFSVVYDRVSLELHNSIFEATKELFHL 70
           LP ID S      G+ +W     ++   LE++GCF   +D++S+EL+ S+FEA ++LF L
Sbjct: 7   LPVIDFSDQNLKPGSSKWDEVTADVLKALEDYGCFEASFDKLSVELNRSVFEAMEDLFEL 66

Query: 71  PLETRIKNTSEKPYHGYFGGYSVLPLYESMGIDNPIELQATQSFANLMWPTKENNHFCGT 130
           P+ T+ +N S K +HGY        LYES+GI++   L+    F   +WP   N     T
Sbjct: 67  PIPTKQRNVSSKLFHGYL----CHNLYESLGINDANVLEKVNDFTQQLWPDHGNKSISET 126

Query: 131 VERFSTLVAELEKMVTKMVLESYGLSKLVNEPNMESANYLLRCFKYRAPEKD----ETDV 190
           +  FS  + EL+ MV +M++ES+G+ K ++E ++ S  YL R  KY +P  D    ET +
Sbjct: 127 IHLFSEQLVELDLMVRRMIMESFGIEKYIDE-HLNSTYYLTRLMKYTSPPDDDDDEETKL 186

Query: 191 GLHSHTDLTFFSIIHQHLISGLQVQSLDDQWIDIQPSGHCYFIVMAGDALMAWSNERIRS 250
           GL SHTD    +I+HQ+ + GL+V++ DD+WI ++PS     +VM GD+L A  N R+ S
Sbjct: 187 GLRSHTDKNIITILHQYQVDGLEVKTKDDKWIKVKPSQDS-VLVMVGDSLCALLNGRLHS 246

Query: 251 CRHRVIMRGEETRYSIGMFSF-KNGTIA-VPEELVDDANPLRYKPFNHYDFLTYDKAKA 303
             HRVIM G++TRYS G+FS  K G I   PEELVD  +P  +KPF + DFL + + +A
Sbjct: 247 PYHRVIMTGKKTRYSTGLFSIPKTGVIIDSPEELVDKEHPRIFKPFEYTDFLHFFQTEA 299

BLAST of Bhi09G000065 vs. ExPASy Swiss-Prot
Match: A0A0N9HQ36 (Deoxypodophyllotoxin synthase OS=Sinopodophyllum hexandrum OX=93608 GN=2-ODD PE=1 SV=1)

HSP 1 Score: 202.2 bits (513), Expect = 8.6e-51
Identity = 114/304 (37.50%), Postives = 162/304 (53.29%), Query Frame = 0

Query: 11  LPTIDLS-KLEAGGTEEWGLACKEMRLGLEEFGCFSVVYDRVSLELHNSIFEATKELFHL 70
           LP IDLS K    GT  W     ++R  LEE+GCF  V D VS EL  ++     EL +L
Sbjct: 9   LPVIDLSMKNLKPGTTSWNSVRTQVREALEEYGCFEAVIDAVSPELQKAVCNKGHELLNL 68

Query: 71  PLETRIKNTSEKPYHGYFGGYSVLPLYESMGIDNPIELQATQSFANLMWPTKENNHFCGT 130
           PLET++ N ++  Y G+    S+  L E MG+    +L+  + F NLMWP + N  FC T
Sbjct: 69  PLETKMLNGNKPEYDGF---TSIPNLNEGMGVGRITDLEKVERFTNLMWP-EGNKDFCET 128

Query: 131 VERFSTLVAELEKMVTKMVLESYGLSKLVNEPNMESANYLLRCFKYRAPEKDETDVGLHS 190
           V  +   +AE++ ++  MV ES+G+ K   +   ES NYLL   +Y+ P KD     L  
Sbjct: 129 VYSYGKRMAEVDHILKMMVFESFGMEKHF-DSFCESTNYLLHFMRYQQPGKDGRSPALSL 188

Query: 191 HTDLTFFSIIHQHLISGLQVQSLDDQWIDIQPSGHCYFIVMAGDALMAWSNERIRSCRHR 250
           H D +  +I++Q+ + GL+ ++ D +WI      H   IV+ GD  MAWSN R+ S  HR
Sbjct: 189 HKDKSILTIVNQNDVKGLEFETKDGEWILPTADNH---IVLLGDCFMAWSNGRLHSPLHR 248

Query: 251 VIMRGEETRYSIGMFSFKNGTIAVPEELVDDANPLRYKPFNHYDFLTYDKAKASHQNVSR 310
           V +   + R S   FSF    I  P ELVD+ +PL + PF   + L Y   K   + V  
Sbjct: 249 VTLVANQARLSTSSFSFPKDIIETPAELVDEEHPLLFNPFEITELLAYCFTKEGAKAVCD 304

Query: 311 IKDY 314
           +K Y
Sbjct: 309 LKQY 304

BLAST of Bhi09G000065 vs. ExPASy Swiss-Prot
Match: Q945B5 (2-oxoglutarate-dependent dioxygenase AOP2 OS=Arabidopsis thaliana OX=3702 GN=AOP2 PE=2 SV=1)

HSP 1 Score: 171.8 bits (434), Expect = 1.2e-41
Identity = 116/427 (27.17%), Postives = 189/427 (44.26%), Query Frame = 0

Query: 11  LPTIDLS-KLEAGGTEEWGLACKEMRLGLEEFGCFSVVYDRVSLELHNSIFEATKELFHL 70
           LP I+L+ K    G+ +W     ++R  LE+FGCF   YD+VSLEL  SI +  +ELF L
Sbjct: 8   LPLINLADKTLEPGSSKWAEVRSDVRKALEDFGCFEASYDKVSLELQESIMKTMEELFAL 67

Query: 71  PLETRIKNTSEKPYHGYFGGYSVLPLYESMGIDNPIELQATQSFANLMWPTKE-NNHFCG 130
           P+ET+ +N   KPY GY    +   L ES+GI N   L+    F   +WP  + N +   
Sbjct: 68  PVETKQRNVCPKPYVGYLNHNN---LSESLGISNANILENVNEFTQQLWPDGDGNENISK 127

Query: 131 TVERFSTLVAELEKMVTKMVLESYGLSKLVNEPNMESANYLLRCFKYRAP---------- 190
           T++ F+  + E++ MV +MV+ES+G+ K +++ +++S  Y +R  KY AP          
Sbjct: 128 TIQLFAEKLVEIDVMVRRMVMESFGIEKYIDD-HLKSTEYRMRLMKYIAPPEGDANTTVD 187

Query: 191 ------------------------------------------------------------ 250
                                                                       
Sbjct: 188 DYADLLAKLNIDGVEPNVGVKVNADISDDVNANPSVNAGVGANVNADTGVNDNLNVDAEA 247

Query: 251 ----------------------------------------------EKDETDVGLHSHTD 310
                                                         + +E  +GL  HTD
Sbjct: 248 NGDANIAVGGGVNANTDLGVGVNVNSNVAVNAKTGATSGDDVEANDDNEEKKLGLPCHTD 307

Query: 311 LTFFSIIHQHLISGLQVQSLDDQWIDIQPSGHCYFIVMAGDALMAWSNERIRSCRHRV-I 317
              F+++ QH I GL+V++ D++WI ++PS +  FIV+AGD+L A  N RIR+  HRV +
Sbjct: 308 KNLFTVLFQHEIEGLEVKTKDEKWIRVKPSPNT-FIVIAGDSLCALMNGRIRAPYHRVRV 367

BLAST of Bhi09G000065 vs. ExPASy TrEMBL
Match: A0A1S3CDV2 (probable 2-oxoglutarate-dependent dioxygenase AOP1 isoform X1 OS=Cucumis melo OX=3656 GN=LOC103499759 PE=3 SV=1)

HSP 1 Score: 568.9 bits (1465), Expect = 1.3e-158
Identity = 271/314 (86.31%), Postives = 291/314 (92.68%), Query Frame = 0

Query: 5   EAKAK-VLPTIDLSKLEAGGTEEWGLACKEMRLGLEEFGCFSVVYDRVSLELHNSIFEAT 64
           EAK+K V+PTIDLSKLE  GT+EWGLACKEMR GLEEFGCFSVVYD+VSLELHNSI EAT
Sbjct: 4   EAKSKVVIPTIDLSKLEV-GTKEWGLACKEMRFGLEEFGCFSVVYDKVSLELHNSIVEAT 63

Query: 65  KELFHLPLETRIKNTSEKPYHGYFGGYSVLPLYESMGIDNPIELQATQSFANLMWPTKEN 124
           KELFHLP+ETR+KNTSEKPYHGYFGGYS LPLYESM IDNPI+L++TQSFANLMWPT++N
Sbjct: 64  KELFHLPIETRMKNTSEKPYHGYFGGYSFLPLYESMAIDNPIQLKSTQSFANLMWPTRDN 123

Query: 125 NHFCGTVERFSTLVAELEKMVTKMVLESYGLSKLVNEPNMESANYLLRCFKYRAPEKDET 184
           NHFC TVERFSTLVA+LEKMVTKMVLESYG+SK+VNEP MES NYLLR FKYR  EKDET
Sbjct: 124 NHFCVTVERFSTLVAKLEKMVTKMVLESYGVSKVVNEPIMESTNYLLRLFKYRVAEKDET 183

Query: 185 DVGLHSHTDLTFFSIIHQHLISGLQVQSL-DDQWIDIQPSGHCYFIVMAGDALMAWSNER 244
           DVGLHSHTDLTFFSIIHQHLISGLQ+QSL DDQW+D+ PS HC FIVMAGDALMAWSNER
Sbjct: 184 DVGLHSHTDLTFFSIIHQHLISGLQIQSLNDDQWLDVHPSSHCSFIVMAGDALMAWSNER 243

Query: 245 IRSCRHRVIMRGEETRYSIGMFSFKNGTIAVPEELVDDANPLRYKPFNHYDFLTYDKAKA 304
           IRSCRHRVIMRGEETRYSIGMFSFKNGTI VP+  VDDANPLRYKPFNHYDFLTYDKA A
Sbjct: 244 IRSCRHRVIMRGEETRYSIGMFSFKNGTIEVPQGFVDDANPLRYKPFNHYDFLTYDKANA 303

Query: 305 SHQNVSRIKDYCGI 317
           SH  +SRIKDYCG+
Sbjct: 304 SHNTISRIKDYCGL 316

BLAST of Bhi09G000065 vs. ExPASy TrEMBL
Match: A0A6J1JXN6 (probable 2-oxoglutarate-dependent dioxygenase AOP1 OS=Cucurbita maxima OX=3661 GN=LOC111488804 PE=3 SV=1)

HSP 1 Score: 492.7 bits (1267), Expect = 1.2e-135
Identity = 231/317 (72.87%), Postives = 268/317 (84.54%), Query Frame = 0

Query: 2   VNSEAKAKVLPTIDLSKLEA--GGTEEWGLACKEMRLGLEEFGCFSVVYDRVSLELHNSI 61
           + SE +A+V P IDLSKLE    GTE WG  CK MR+GLEEFGCF VV++ VSLELHNS 
Sbjct: 1   MGSETQARV-PVIDLSKLETLEAGTEAWGSTCKAMRVGLEEFGCFCVVFEEVSLELHNSF 60

Query: 62  FEATKELFHLPLETRIKNTSEKPYHGYFGGYSVLPLYESMGIDNPIELQATQSFANLMWP 121
           F A  ELF LP+ETRIKNTSEK YHGYFG YS LPLYESM I+NP +L A QSFANLMWP
Sbjct: 61  FGAVDELFQLPIETRIKNTSEKAYHGYFGRYSFLPLYESMAIENPTQLDAAQSFANLMWP 120

Query: 122 TKENNHFCGTVERFSTLVAELEKMVTKMVLESYGLSKLVNEPNMESANYLLRCFKYRAPE 181
            + NNHF  T++RFSTL+A+L+K++T+MVLESYG++K + + N++S   LLR FKYR PE
Sbjct: 121 AQGNNHFSETIQRFSTLMAKLDKIITRMVLESYGVTKQLIDTNIDSTANLLRFFKYRVPE 180

Query: 182 KDETDVGLHSHTDLTFFSIIHQHLISGLQVQSLDDQWIDIQPSGHCYFIVMAGDALMAWS 241
           + ETDVGLH+HTDLTFFSIIHQHLI GLQ+QSLDDQWID++PSGHC F+VMAGDALMAWS
Sbjct: 181 EHETDVGLHAHTDLTFFSIIHQHLIGGLQIQSLDDQWIDLEPSGHCSFVVMAGDALMAWS 240

Query: 242 NERIRSCRHRVIMRGEETRYSIGMFSFKNGTIAVPEELVDDANPLRYKPFNHYDFLTYDK 301
           N+RIRSCRHRV M+ EETRYS GMFSFKNGTI+VPEELVDDANPLRYKPFNH+DFLTYDK
Sbjct: 241 NDRIRSCRHRVTMKAEETRYSAGMFSFKNGTISVPEELVDDANPLRYKPFNHFDFLTYDK 300

Query: 302 AKASHQNVSRIKDYCGI 317
           AK S + + RI+DYC I
Sbjct: 301 AKGSKKFLYRIRDYCSI 316

BLAST of Bhi09G000065 vs. ExPASy TrEMBL
Match: A0A6J1GPR2 (probable 2-oxoglutarate-dependent dioxygenase AOP1 OS=Cucurbita moschata OX=3662 GN=LOC111456352 PE=3 SV=1)

HSP 1 Score: 488.0 bits (1255), Expect = 2.9e-134
Identity = 233/317 (73.50%), Postives = 265/317 (83.60%), Query Frame = 0

Query: 2   VNSEAKAKVLPTIDLSKLE--AGGTEEWGLACKEMRLGLEEFGCFSVVYDRVSLELHNSI 61
           + SE +A V P IDLSKLE    GT+ WG  CK MR+GLEEFGCF VV++ VSLELHNS 
Sbjct: 1   MGSETQAGV-PVIDLSKLEKLEAGTDAWGSTCKAMRVGLEEFGCFCVVFEEVSLELHNSF 60

Query: 62  FEATKELFHLPLETRIKNTSEKPYHGYFGGYSVLPLYESMGIDNPIELQATQSFANLMWP 121
           F A  ELF LP+ETRIKNTSEK YHGYFG YS LPLYESM IDNP +L A QSFANLMWP
Sbjct: 61  FGAVDELFQLPIETRIKNTSEKAYHGYFGRYSFLPLYESMAIDNPTQLDAAQSFANLMWP 120

Query: 122 TKENNHFCGTVERFSTLVAELEKMVTKMVLESYGLSKLVNEPNMESANYLLRCFKYRAPE 181
            + NN F  T++RFSTLVA+L+K+VT+MVLESYG+SK + + N++S   LLR FKYR PE
Sbjct: 121 DQGNNRFSETIQRFSTLVAKLDKIVTRMVLESYGVSKQLIDTNIDSTANLLRFFKYRVPE 180

Query: 182 KDETDVGLHSHTDLTFFSIIHQHLISGLQVQSLDDQWIDIQPSGHCYFIVMAGDALMAWS 241
           + ETDVGLH+HTDLTFFSIIHQHLI GLQ+QSLDDQWID++PS HC FIVMAGDALMAWS
Sbjct: 181 QHETDVGLHAHTDLTFFSIIHQHLIGGLQIQSLDDQWIDLEPSSHCSFIVMAGDALMAWS 240

Query: 242 NERIRSCRHRVIMRGEETRYSIGMFSFKNGTIAVPEELVDDANPLRYKPFNHYDFLTYDK 301
           N+RIRSCRHRV M+ EETRYS GMFSFKNGTI+VPEELVDDANPLRYKPFNHYDFLT+DK
Sbjct: 241 NDRIRSCRHRVTMKAEETRYSAGMFSFKNGTISVPEELVDDANPLRYKPFNHYDFLTHDK 300

Query: 302 AKASHQNVSRIKDYCGI 317
           AK S + + RI+DYC I
Sbjct: 301 AKGSKKFLYRIRDYCSI 316

BLAST of Bhi09G000065 vs. ExPASy TrEMBL
Match: A0A6J1CXE5 (probable 2-oxoglutarate-dependent dioxygenase AOP1 OS=Momordica charantia OX=3673 GN=LOC111015077 PE=3 SV=1)

HSP 1 Score: 468.8 bits (1205), Expect = 1.8e-128
Identity = 227/321 (70.72%), Postives = 271/321 (84.42%), Query Frame = 0

Query: 2   VNSEAKAKVLPTIDLS--KLEAGGTEEWGLACKEMRLGLEEFGCFSVVYDRVSLELHNSI 61
           + ++AK+K+ PTI+LS  KL+  G+EEW L  KE+R+ LEEFGCF VVY++VSLELHNSI
Sbjct: 1   MGTQAKSKI-PTIELSEEKLKV-GSEEWVLIGKEIRVALEEFGCFLVVYEKVSLELHNSI 60

Query: 62  FEATKELFHLPLETRIKNTSEKPYHGYFGGYSVLPLYESMGIDNPIELQATQSFANLMWP 121
           FEATKELFHLP+ETRI+NTSEKPYHGYFG YS LPLYESM +DNP  LQA Q+F NLMWP
Sbjct: 61  FEATKELFHLPIETRIQNTSEKPYHGYFGRYSFLPLYESMAVDNPTSLQAIQTFTNLMWP 120

Query: 122 TKENNHFCGTVERFSTLVAELEKMVTKMVLESYGLSKLVNEPNMESANYLLRCFKYRAPE 181
              N  FC TV  FSTLVA+LE+ VT+MVLESYG+SK V E ++ES  +LLRCFKYR P+
Sbjct: 121 A-GNKTFCETVHDFSTLVAKLEETVTRMVLESYGVSKQVCESSIESTTHLLRCFKYRVPK 180

Query: 182 KDETDVGLHSHTDLTFFSIIHQHLISGLQVQSLDD----QWIDIQPSGHCYFIVMAGDAL 241
           +DETD+GLHSHTDLTF SI+HQHLI GLQV+SLD+    +WIDI P G   FIVMAGDAL
Sbjct: 181 QDETDLGLHSHTDLTFLSILHQHLIGGLQVKSLDNNNDGEWIDIDPVGRSSFIVMAGDAL 240

Query: 242 MAWSNERIRSCRHRVIMRGEETRYSIGMFSFKNGTIAVPEELVDDANPLRYKPFNHYDFL 301
           MAWSN+RIR CRHRV+MRG+ETRYS+G+FSFK+GTI+VPEELV+D +PL+YKPF+H++FL
Sbjct: 241 MAWSNDRIRGCRHRVMMRGDETRYSLGLFSFKSGTISVPEELVEDNHPLKYKPFDHFEFL 300

Query: 302 TYDKAKASHQNVSRIKDYCGI 317
            YDKA ASH+  SRIKDYCGI
Sbjct: 301 RYDKANASHKTCSRIKDYCGI 318

BLAST of Bhi09G000065 vs. ExPASy TrEMBL
Match: A0A5A7USF0 (Putative 2-oxoglutarate-dependent dioxygenase AOP1 isoform X1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold459G002400 PE=3 SV=1)

HSP 1 Score: 449.9 bits (1156), Expect = 8.8e-123
Identity = 209/243 (86.01%), Postives = 225/243 (92.59%), Query Frame = 0

Query: 75  IKNTSEKPYHGYFGGYSVLPLYESMGIDNPIELQATQSFANLMWPTKENNHFCGTVERFS 134
           +KNTSEKPYHGYFGGYS LPLYESM IDNPI+L++TQSFANLMWPT++NNHFC TVERFS
Sbjct: 1   MKNTSEKPYHGYFGGYSFLPLYESMAIDNPIQLKSTQSFANLMWPTRDNNHFCVTVERFS 60

Query: 135 TLVAELEKMVTKMVLESYGLSKLVNEPNMESANYLLRCFKYRAPEKDETDVGLHSHTDLT 194
           TLVA+LEKMVTKMVLESYG+SK+VNEP MES NYLLR FKYR  EKDETDVGLHSHTDLT
Sbjct: 61  TLVAKLEKMVTKMVLESYGVSKVVNEPIMESTNYLLRLFKYRVAEKDETDVGLHSHTDLT 120

Query: 195 FFSIIHQHLISGLQVQSL-DDQWIDIQPSGHCYFIVMAGDALMAWSNERIRSCRHRVIMR 254
           FFSIIHQHLISGLQ+QSL DDQW+D+ PS HC FIVMAGDALMAWSNERIRSCRH+VIMR
Sbjct: 121 FFSIIHQHLISGLQIQSLNDDQWLDVHPSSHCSFIVMAGDALMAWSNERIRSCRHKVIMR 180

Query: 255 GEETRYSIGMFSFKNGTIAVPEELVDDANPLRYKPFNHYDFLTYDKAKASHQNVSRIKDY 314
           GEETRYSIGMFSFKNGTI VP+  VDDANPLRYKPFNHYDFLTYDKA ASH  +SRIKDY
Sbjct: 181 GEETRYSIGMFSFKNGTIEVPQGFVDDANPLRYKPFNHYDFLTYDKANASHNTISRIKDY 240

Query: 315 CGI 317
           CG+
Sbjct: 241 CGL 243

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
AT1G52800.15.0e-7845.192-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein [more]
AT1G52790.13.9e-7543.652-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein [more]
AT1G52820.11.8e-7242.392-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein [more]
AT1G80320.17.7e-6339.492-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein [more]
AT1G15540.11.3e-6241.642-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein [more]
Match NameE-valueIdentityDescription
Q945B63.5e-6041.00Probable 2-oxoglutarate-dependent dioxygenase AOP1.2 OS=Arabidopsis thaliana OX=... [more]
Q9ZTA31.3e-5940.53Probable 2-oxoglutarate-dependent dioxygenase AOP1 OS=Arabidopsis thaliana OX=37... [more]
Q944Z53.0e-5940.80Probable 2-oxoglutarate-dependent dioxygenase AOP1 (Fragment) OS=Arabidopsis tha... [more]
A0A0N9HQ368.6e-5137.50Deoxypodophyllotoxin synthase OS=Sinopodophyllum hexandrum OX=93608 GN=2-ODD PE=... [more]
Q945B51.2e-4127.172-oxoglutarate-dependent dioxygenase AOP2 OS=Arabidopsis thaliana OX=3702 GN=AOP... [more]
Match NameE-valueIdentityDescription
A0A1S3CDV21.3e-15886.31probable 2-oxoglutarate-dependent dioxygenase AOP1 isoform X1 OS=Cucumis melo OX... [more]
A0A6J1JXN61.2e-13572.87probable 2-oxoglutarate-dependent dioxygenase AOP1 OS=Cucurbita maxima OX=3661 G... [more]
A0A6J1GPR22.9e-13473.50probable 2-oxoglutarate-dependent dioxygenase AOP1 OS=Cucurbita moschata OX=3662... [more]
A0A6J1CXE51.8e-12870.72probable 2-oxoglutarate-dependent dioxygenase AOP1 OS=Momordica charantia OX=367... [more]
A0A5A7USF08.8e-12386.01Putative 2-oxoglutarate-dependent dioxygenase AOP1 isoform X1 OS=Cucumis melo va... [more]
InterPro
Analysis Name: InterPro Annotations of Wax gourd (B227) v1
Date Performed: 2021-10-22
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR026992Non-haem dioxygenase N-terminal domainPFAMPF14226DIOX_Ncoord: 11..88
e-value: 5.5E-11
score: 43.2
IPR044861Isopenicillin N synthase-like, Fe(2+) 2OG dioxygenase domainPFAMPF031712OG-FeII_Oxycoord: 175..266
e-value: 9.4E-16
score: 58.2
IPR027443Isopenicillin N synthase-like superfamilyGENE3D2.60.120.330coord: 1..305
e-value: 4.3E-60
score: 205.7
NoneNo IPR availablePANTHERPTHR47990:SF1692-OXOGLUTARATE-DEPENDENT DIOXYGENASEcoord: 5..315
NoneNo IPR availablePANTHERPTHR479902-OXOGLUTARATE (2OG) AND FE(II)-DEPENDENT OXYGENASE SUPERFAMILY PROTEIN-RELATEDcoord: 5..315
NoneNo IPR availableSUPERFAMILY51197Clavaminate synthase-likecoord: 11..297
IPR005123Oxoglutarate/iron-dependent dioxygenasePROSITEPS51471FE2OG_OXYcoord: 165..269
score: 9.855806

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Bhi09M000065Bhi09M000065mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0051213 dioxygenase activity
molecular_function GO:0046872 metal ion binding