Bhi06G001376 (gene) Wax gourd (B227) v1

Overview
NameBhi06G001376
Typegene
OrganismBenincasa hispida (Wax gourd (B227) v1)
DescriptionG protein gamma domain-containing protein
Locationchr6: 46130673 .. 46132661 (+)
RNA-Seq ExpressionBhi06G001376
SyntenyBhi06G001376
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGACTACTCCCACTCGCCCACCTTCCTCTTCTTCCTCTTCAATTCCATCCCTCCCACCCCCTTACCCCAAGTCGCCTCCTGAGTATCCAGATTTGTATGGAAAGCGCCGACAAGCCGCCAAGGTTCAGATGCTCGAGAGGGAGATTGGTTTTCTCGAGGTTCATTCCGTTTTTCTCTGCTTTTTCTTTTAACTTCTGATGCATGCGAATGCCCTTTCTTTCGTGAGTCTGAAGAAACTGGTTCTCCTTCACAACCTGATTGAAACCCAAGTCCCGGGTTCCAGTGCCATTTTAGCGAGCATCCCATTTGATGTAATTACAACCTAAGGCTCTCGTAGATGTATGCATAGGGACCGAAATTGACAACGTTTTGCTGTCCATTTCAGATCCCCCATTGTTGAGTTTTGAAGACCTTTTAGGGGTTTGTCCCTTGTATTGTTAGGGTCAGTTAAGGATGGGGTGGGGTACCGACTGGGGCATCCCTCTCAAGTCACACTTCACCGTTGTATGTCCTCAGCTCTCAATATGTTACAAACCAAAAGGAGTTTTTATTTGCACTCTTGTCCCAAAATCATTCACCCCTTTGATTTTGTCTCTTTCTTTTCTTCTAAAAGATTCTCGTTGAAGGTCTAAGTTAGCTGTTTTATTACATTTTGTTCCTTTCCCTCGAACATTTGAGCTCTTGTTTCAATATATTGAATACCCTAAGTCATCTTTCCCTACAAAGTTTAAGGAAAAAAGTATGGGAACTGAACACTACCTATGAAATTGTCGATGCTTCTTCAAATTTAGATTTGAGTTTTGCTTACAAGAAAAATCCCATTGCCATTGATGTTCAACCTAACCATTTGGGTTTATTTCCTGCATTGCTTTCTCCTAACTTCCTGATTCATGATGAAAAATTAACAAGCTTTTCTTGGCAATGTCTCTGGAGCAGGAAGAATTGAAGTCTACTGAAGGTCTTCAACCTGCATCTAAATGCTGCAAAGAGTGTGTATTTATTTTCAGAATACTTTGAACTTTTACTTCGGAATGGTTTTTTGCCCATCCAGTTGGCTACCATTTAGTTGATTTGTGTTTTTCTTACACTTTGCAGAGTAGCTGAGTATGTGATCGCCAACTCAGATCCCTTGATACCAACGTAAGGGGTTTCTCATCATTTTAATTTTCTAAAATTCCTTGTAATTTATTATTCACGCTACATGTTTCTTTTAATCAGATACAAGAAGCATCGCCGCAGATGTCAATTTTGGAAATGGTTCTGGTATGACTCTCGCCTCATCATTTATTCCTTCCTATGGAGTAGAAATAATAAGTGAATTGACCCAAGTTTCTCTCACTTTTGCTATTTGTCTAATCTAGCTTCCCAGTTGTAGTCTTAGGTTAACGTGAAGTAATAAAGCATTGCCTACATTTGGCTTATAGCGAGCAATCTCTAATTAGACAATAGGTGTCATTTGTGCTTGGAAATTAAGAAGATACTAGCAGAATTAAATACTATGCCATTTACTACTTAAAATAATAGCCTGGACTTCCCTCACTACCTTTGCTAATGAAGCAGAAATGTCTCCCATGTCGTATGTAGACTCTGTATTTTGTGTAGTAGGGGTATCGGCTGGTGCATTTGACACAGGTTAATTCTCACTTGATGCAGTGGATTCCCCTGTTGTAAGTTATCCTGGATTTGCTGTTGCTGTTATGCTGGATGCTCGATCCATCTCGAAATCCCACCCTGCTGCAACTGTAATAACCCATGTAAATGCCTATGTTGCGGTAGCTGTAAATTACCGAAGTGGCAGTGTTGCGGGGAGTGCAGGGAGTGTTGTGGCCAGTGTGGGCATTGTTGCACTTGTCCAACACTACCCCAATGCTCATGCCTTTGTCCAACCATCTCATGCAGCCAATGTTGTAGCAATTTTTCATTACCATCCTGCTCTTGCCCGGAATGCCCATGCTGTGAATGCAAATGCAAATGCAAATGTACGTTAG

mRNA sequence

ATGACTACTCCCACTCGCCCACCTTCCTCTTCTTCCTCTTCAATTCCATCCCTCCCACCCCCTTACCCCAAGTCGCCTCCTGAGTATCCAGATTTGTATGGAAAGCGCCGACAAGCCGCCAAGGTTCAGATGCTCGAGAGGGAGATTGGTTTTCTCGAGGAAGAATTGAAGTCTACTGAAGGTCTTCAACCTGCATCTAAATGCTGCAAAGAAGTAGCTGAGTATGTGATCGCCAACTCAGATCCCTTGATACCAACAAGCATCGCCGCAGATGTCAATTTTGGAAATGGTTCTGTGGATTCCCCTGTTGTAAGTTATCCTGGATTTGCTGTTGCTGTTATGCTGGATGCTCGATCCATCTCGAAATCCCACCCTGCTGCAACTGTAATAACCCATGTAAATGCCTATGTTGCGGTAGCTGTAAATTACCGAAGTGGCAGTGTTGCGGGGAGTGCAGGGAGTGTTGTGGCCAGTGTGGGCATTGTTGCACTTGTCCAACACTACCCCAATGCTCATGCCTTTGTCCAACCATCTCATGCAGCCAATGTTGTAGCAATTTTTCATTACCATCCTGCTCTTGCCCGGAATGCCCATGCTGTGAATGCAAATGCAAATGCAAATGTACGTTAG

Coding sequence (CDS)

ATGACTACTCCCACTCGCCCACCTTCCTCTTCTTCCTCTTCAATTCCATCCCTCCCACCCCCTTACCCCAAGTCGCCTCCTGAGTATCCAGATTTGTATGGAAAGCGCCGACAAGCCGCCAAGGTTCAGATGCTCGAGAGGGAGATTGGTTTTCTCGAGGAAGAATTGAAGTCTACTGAAGGTCTTCAACCTGCATCTAAATGCTGCAAAGAAGTAGCTGAGTATGTGATCGCCAACTCAGATCCCTTGATACCAACAAGCATCGCCGCAGATGTCAATTTTGGAAATGGTTCTGTGGATTCCCCTGTTGTAAGTTATCCTGGATTTGCTGTTGCTGTTATGCTGGATGCTCGATCCATCTCGAAATCCCACCCTGCTGCAACTGTAATAACCCATGTAAATGCCTATGTTGCGGTAGCTGTAAATTACCGAAGTGGCAGTGTTGCGGGGAGTGCAGGGAGTGTTGTGGCCAGTGTGGGCATTGTTGCACTTGTCCAACACTACCCCAATGCTCATGCCTTTGTCCAACCATCTCATGCAGCCAATGTTGTAGCAATTTTTCATTACCATCCTGCTCTTGCCCGGAATGCCCATGCTGTGAATGCAAATGCAAATGCAAATGTACGTTAG

Protein sequence

MTTPTRPPSSSSSSIPSLPPPYPKSPPEYPDLYGKRRQAAKVQMLEREIGFLEEELKSTEGLQPASKCCKEVAEYVIANSDPLIPTSIAADVNFGNGSVDSPVVSYPGFAVAVMLDARSISKSHPAATVITHVNAYVAVAVNYRSGSVAGSAGSVVASVGIVALVQHYPNAHAFVQPSHAANVVAIFHYHPALARNAHAVNANANANVR
Homology
BLAST of Bhi06G001376 vs. TAIR 10
Match: AT5G20635.1 (FUNCTIONS IN: molecular_function unknown; INVOLVED IN: biological_process unknown; LOCATED IN: keratin filament, chloroplast; CONTAINS InterPro DOMAIN/s: Keratin, high sulphur B2 protein (InterPro:IPR002494); Has 6153 Blast hits to 2882 proteins in 265 species: Archae - 6; Bacteria - 47; Metazoa - 4777; Fungi - 68; Plants - 187; Viruses - 30; Other Eukaryotes - 1038 (source: NCBI BLink). )

HSP 1 Score: 118.2 bits (295), Expect = 7.7e-27
Identity = 59/77 (76.62%), Postives = 69/77 (89.61%), Query Frame = 0

Query: 9  SSSSSSIPSLPPPYPKSPPEYPDLYGKRRQAAKVQMLEREIGFLEEELKSTEGLQPASKC 68
          SSSS +  SLPPP PKSPPEYPDLYGKRR+AA+VQMLEREIGFLE E+K  EG+QPAS+C
Sbjct: 21 SSSSLAPSSLPPPRPKSPPEYPDLYGKRREAARVQMLEREIGFLEGEIKFIEGVQPASRC 80

Query: 69 CKEVAEYVIANSDPLIP 86
           KEV+++V+ANSDPLIP
Sbjct: 81 IKEVSDFVVANSDPLIP 97

BLAST of Bhi06G001376 vs. TAIR 10
Match: AT3G22942.1 (G-protein gamma subunit 2 )

HSP 1 Score: 48.5 bits (114), Expect = 7.5e-06
Identity = 25/55 (45.45%), Postives = 34/55 (61.82%), Query Frame = 0

Query: 31 DLYGKRRQAAKVQMLEREIGFLEEELKSTEGLQPASKCCKEVAEYVIANSDPLIP 86
          D  GK R  A+++ LE+E  FLEEEL+  E +  AS  CKE  + V +  DPL+P
Sbjct: 18 DTRGKHRIQAELKRLEQEARFLEEELEQLEKMDNASASCKEFLDSVDSKPDPLLP 72

BLAST of Bhi06G001376 vs. ExPASy Swiss-Prot
Match: Q6AWT8 (Guanine nucleotide-binding protein subunit gamma 3 OS=Arabidopsis thaliana OX=3702 GN=GG3 PE=2 SV=1)

HSP 1 Score: 118.2 bits (295), Expect = 1.1e-25
Identity = 59/77 (76.62%), Postives = 69/77 (89.61%), Query Frame = 0

Query: 9  SSSSSSIPSLPPPYPKSPPEYPDLYGKRRQAAKVQMLEREIGFLEEELKSTEGLQPASKC 68
          SSSS +  SLPPP PKSPPEYPDLYGKRR+AA+VQMLEREIGFLE E+K  EG+QPAS+C
Sbjct: 21 SSSSLAPSSLPPPRPKSPPEYPDLYGKRREAARVQMLEREIGFLEGEIKFIEGVQPASRC 80

Query: 69 CKEVAEYVIANSDPLIP 86
           KEV+++V+ANSDPLIP
Sbjct: 81 IKEVSDFVVANSDPLIP 97

BLAST of Bhi06G001376 vs. ExPASy Swiss-Prot
Match: Q93V47 (Guanine nucleotide-binding protein subunit gamma 2 OS=Arabidopsis thaliana OX=3702 GN=GG2 PE=1 SV=1)

HSP 1 Score: 48.5 bits (114), Expect = 1.1e-04
Identity = 25/55 (45.45%), Postives = 34/55 (61.82%), Query Frame = 0

Query: 31 DLYGKRRQAAKVQMLEREIGFLEEELKSTEGLQPASKCCKEVAEYVIANSDPLIP 86
          D  GK R  A+++ LE+E  FLEEEL+  E +  AS  CKE  + V +  DPL+P
Sbjct: 18 DTRGKHRIQAELKRLEQEARFLEEELEQLEKMDNASASCKEFLDSVDSKPDPLLP 72

BLAST of Bhi06G001376 vs. ExPASy TrEMBL
Match: A0A5A7UZX5 (Guanine nucleotide-binding protein subunit gamma 3-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold332G001280 PE=4 SV=1)

HSP 1 Score: 290.0 bits (741), Expect = 7.7e-75
Identity = 170/229 (74.24%), Postives = 181/229 (79.04%), Query Frame = 0

Query: 3   TPTRPPSSSSSSIPSLPPPYPKSPPEYPDLYGKRRQAAKVQMLEREIGFLEEELKSTEGL 62
           TPTRPPSSSSSS  S+PPPYPKSPP+YPDLYGKRRQAAK+QML+REIGFLEEELKSTEGL
Sbjct: 4   TPTRPPSSSSSS--SIPPPYPKSPPQYPDLYGKRRQAAKLQMLDREIGFLEEELKSTEGL 63

Query: 63  QPASKCCKEVAEYVIANSDPLIPTS----------------------IAADVNFGNGSVD 122
           QPASKCCKEVA+YVIANSDP+IPT                       + A +N     VD
Sbjct: 64  QPASKCCKEVADYVIANSDPMIPTYKKHRRRCQFWKWLCNKALPKIWLIAIINL-KLEVD 123

Query: 123 SPVVSYPGFAVAVMLDARSISKSHPAATVITHVNAYVAVAVNYRSGSVAGSAGSVVASVG 182
           SPVVSYPGFAVAVMLDARSISKS PAATVIT VNAY   AVNYRSG V    GSVV SVG
Sbjct: 124 SPVVSYPGFAVAVMLDARSISKSLPAATVITLVNAYRVAAVNYRSGCV---VGSVVGSVG 183

Query: 183 IVALVQHYPNAHAFVQPSHAANVVAIFHYHPALARNAHAVNANANANVR 210
           IVALVQHYPNAHA VQPSHAANVVAIFHY PA A++ HAVNANANAN +
Sbjct: 184 IVALVQHYPNAHALVQPSHAANVVAIFHYLPAPAQSGHAVNANANANAQ 226

BLAST of Bhi06G001376 vs. ExPASy TrEMBL
Match: A0A1S3CB82 (guanine nucleotide-binding protein subunit gamma 3-like OS=Cucumis melo OX=3656 GN=LOC103498849 PE=4 SV=1)

HSP 1 Score: 152.5 bits (384), Expect = 1.9e-33
Identity = 76/84 (90.48%), Postives = 82/84 (97.62%), Query Frame = 0

Query: 3  TPTRPPSSSSSSIPSLPPPYPKSPPEYPDLYGKRRQAAKVQMLEREIGFLEEELKSTEGL 62
          TPTRPPSSSSSS  S+PPPYPKSPP+YPDLYGKRRQAAK+QML+REIGFLEEELKSTEGL
Sbjct: 4  TPTRPPSSSSSSSSSIPPPYPKSPPQYPDLYGKRRQAAKLQMLDREIGFLEEELKSTEGL 63

Query: 63 QPASKCCKEVAEYVIANSDPLIPT 87
          QPASKCCKEVA+YVIANSDP+IPT
Sbjct: 64 QPASKCCKEVADYVIANSDPMIPT 87

BLAST of Bhi06G001376 vs. ExPASy TrEMBL
Match: A0A6J1KGS0 (guanine nucleotide-binding protein subunit gamma 3-like isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111493745 PE=4 SV=1)

HSP 1 Score: 149.1 bits (375), Expect = 2.1e-32
Identity = 77/86 (89.53%), Postives = 82/86 (95.35%), Query Frame = 0

Query: 1  MTTPTRPPSSSSSSIPSLPPPYPKSPPEYPDLYGKRRQAAKVQMLEREIGFLEEELKSTE 60
          M TPTRP  +SSSSIPSLPPPYPKSPP+YPDLYGKRRQAAKVQMLEREIGFLEEELKSTE
Sbjct: 1  MATPTRP--ASSSSIPSLPPPYPKSPPQYPDLYGKRRQAAKVQMLEREIGFLEEELKSTE 60

Query: 61 GLQPASKCCKEVAEYVIANSDPLIPT 87
          GL PAS+CCKEVA++VIANSDPLIPT
Sbjct: 61 GLPPASRCCKEVADHVIANSDPLIPT 84

BLAST of Bhi06G001376 vs. ExPASy TrEMBL
Match: A0A6J1KJF9 (guanine nucleotide-binding protein subunit gamma 3-like isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111493745 PE=4 SV=1)

HSP 1 Score: 149.1 bits (375), Expect = 2.1e-32
Identity = 77/86 (89.53%), Postives = 82/86 (95.35%), Query Frame = 0

Query: 1  MTTPTRPPSSSSSSIPSLPPPYPKSPPEYPDLYGKRRQAAKVQMLEREIGFLEEELKSTE 60
          M TPTRP  +SSSSIPSLPPPYPKSPP+YPDLYGKRRQAAKVQMLEREIGFLEEELKSTE
Sbjct: 1  MATPTRP--ASSSSIPSLPPPYPKSPPQYPDLYGKRRQAAKVQMLEREIGFLEEELKSTE 60

Query: 61 GLQPASKCCKEVAEYVIANSDPLIPT 87
          GL PAS+CCKEVA++VIANSDPLIPT
Sbjct: 61 GLPPASRCCKEVADHVIANSDPLIPT 84

BLAST of Bhi06G001376 vs. ExPASy TrEMBL
Match: A0A6J1G3W8 (guanine nucleotide-binding protein subunit gamma 3-like isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111450505 PE=4 SV=1)

HSP 1 Score: 149.1 bits (375), Expect = 2.1e-32
Identity = 77/86 (89.53%), Postives = 82/86 (95.35%), Query Frame = 0

Query: 1  MTTPTRPPSSSSSSIPSLPPPYPKSPPEYPDLYGKRRQAAKVQMLEREIGFLEEELKSTE 60
          M TPTRP  +SSSSIPSLPPPYPKSPP+YPDLYGKRRQAAKVQMLEREIGFLEEELKSTE
Sbjct: 1  MATPTRP--ASSSSIPSLPPPYPKSPPQYPDLYGKRRQAAKVQMLEREIGFLEEELKSTE 60

Query: 61 GLQPASKCCKEVAEYVIANSDPLIPT 87
          GL PAS+CCKEVA++VIANSDPLIPT
Sbjct: 61 GLPPASRCCKEVADHVIANSDPLIPT 84

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
AT5G20635.17.7e-2776.62FUNCTIONS IN: molecular_function unknown; INVOLVED IN: biological_process unknow... [more]
AT3G22942.17.5e-0645.45G-protein gamma subunit 2 [more]
Match NameE-valueIdentityDescription
Q6AWT81.1e-2576.62Guanine nucleotide-binding protein subunit gamma 3 OS=Arabidopsis thaliana OX=37... [more]
Q93V471.1e-0445.45Guanine nucleotide-binding protein subunit gamma 2 OS=Arabidopsis thaliana OX=37... [more]
Match NameE-valueIdentityDescription
A0A5A7UZX57.7e-7574.24Guanine nucleotide-binding protein subunit gamma 3-like OS=Cucumis melo var. mak... [more]
A0A1S3CB821.9e-3390.48guanine nucleotide-binding protein subunit gamma 3-like OS=Cucumis melo OX=3656 ... [more]
A0A6J1KGS02.1e-3289.53guanine nucleotide-binding protein subunit gamma 3-like isoform X1 OS=Cucurbita ... [more]
A0A6J1KJF92.1e-3289.53guanine nucleotide-binding protein subunit gamma 3-like isoform X2 OS=Cucurbita ... [more]
A0A6J1G3W82.1e-3289.53guanine nucleotide-binding protein subunit gamma 3-like isoform X1 OS=Cucurbita ... [more]
InterPro
Analysis Name: InterPro Annotations of Wax gourd (B227) v1
Date Performed: 2021-10-22
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 35..62
NoneNo IPR availableSMARTSM01224G_gamma_2coord: 38..97
e-value: 8.8E-5
score: 31.9
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1..35
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 13..28
NoneNo IPR availablePANTHERPTHR32378GUANINE NUCLEOTIDE-BINDING PROTEIN SUBUNIT GAMMA 3coord: 8..87
NoneNo IPR availablePANTHERPTHR32378:SF10GUANINE NUCLEOTIDE-BINDING PROTEIN SUBUNIT GAMMA 3coord: 8..87
IPR015898G-protein gamma-like domainPFAMPF00631G-gammacoord: 40..86
e-value: 2.2E-10
score: 40.4

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Bhi06M001376Bhi06M001376mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0007186 G protein-coupled receptor signaling pathway