Bhi06G000757 (gene) Wax gourd (B227) v1

Overview


Name	Bhi06G000757
Type	gene
Organism	Benincasa hispida (Wax gourd (B227) v1)
Description	cytochrome P450 704B1
Location	chr6: 22597584 .. 22598200 (-)
RNA-Seq Expression	Bhi06G000757
Synteny	Bhi06G000757

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGAAACTGAGTTGGAAAAGAGAAAGCAAGAAGGCCCATATAATGGGCGGCCAATTCGAGGGGCAGTCACATTTCTTCGGGTACTTATTCTATTCGTTCTTGAACTTTTGCTGTTTTAGTTTTTCAAATATAGAAAAATAAACAAAAATATTTATAAATATAGTAAAAATTTACTATCTATCTGTAATAGACTGTAATAGACTACTATCTGTGTTTATCTGTATGATAGATAGTGATATTTTTCTATTATCTGTAAATATTTTTAACAGTTTTGTCATTTAAAAAATATATATTTTTAAATTTTTTTAAATTGGGTGTAGGAATTGTTGATGAGAATGACTTTGGACTCCATATGCAAAGTGGGATTTGGTGTTGAAATTGGATCATTGGCTCCAAATTTCCTTGAAAATAGCTTTGCTAAGGCCTTTGACACAGCCAACATGATAGTCACCCTTCGCTTCATCGATCCTTTATGGAAAATTAAAAGATTTCTGAATTGGGGATCTGAAGTTCTTGATAAATCCATCAAAATCATTGATGACTTCACCTATTCTGTCATTGCCACAAGAAAAAAAGAGATCCAAGAAGCTCAAACCACAAGCAAAGATGCCAATTAG

mRNA sequence

ATGAAACTGAGTTGGAAAAGAGAAAGCAAGAAGGCCCATATAATGGGCGGCCAATTCGAGGGGCAAATGACTTTGGACTCCATATGCAAAGTGGGATTTGGTGTTGAAATTGGATCATTGGCTCCAAATTTCCTTGAAAATAGCTTTGCTAAGGCCTTTGACACAGCCAACATGATAGTCACCCTTCGCTTCATCGATCCTTTATGGAAAATTAAAAGATTTCTGAATTGGGGATCTGAAGTTCTTGATAAATCCATCAAAATCATTGATGACTTCACCTATTCTGTCATTGCCACAAGAAAAAAAGAGATCCAAGAAGCTCAAACCACAAGCAAAGATGCCAATTAG

Coding sequence (CDS)

ATGAAACTGAGTTGGAAAAGAGAAAGCAAGAAGGCCCATATAATGGGCGGCCAATTCGAGGGGCAAATGACTTTGGACTCCATATGCAAAGTGGGATTTGGTGTTGAAATTGGATCATTGGCTCCAAATTTCCTTGAAAATAGCTTTGCTAAGGCCTTTGACACAGCCAACATGATAGTCACCCTTCGCTTCATCGATCCTTTATGGAAAATTAAAAGATTTCTGAATTGGGGATCTGAAGTTCTTGATAAATCCATCAAAATCATTGATGACTTCACCTATTCTGTCATTGCCACAAGAAAAAAAGAGATCCAAGAAGCTCAAACCACAAGCAAAGATGCCAATTAG

Protein sequence

MKLSWKRESKKAHIMGGQFEGQMTLDSICKVGFGVEIGSLAPNFLENSFAKAFDTANMIVTLRFIDPLWKIKRFLNWGSEVLDKSIKIIDDFTYSVIATRKKEIQEAQTTSKDAN

Homology

[Close]

BLAST of Bhi06G000757 vs. TAIR 10
Match: AT1G69500.1 (cytochrome P450, family 704, subfamily B, polypeptide 1 )

HSP 1 Score: 134.8 bits (338), Expect = 4.4e-32
Identity = 68/95 (71.58%), Postives = 80/95 (84.21%), Query Frame = 0


Query: 22  QMTLDSICKVGFGVEIGSLAPNFLENSFAKAFDTANMIVTLRFIDPLWKIKRFLNWGSE- 81
           +MTLDSICKVGFGVEIG+LAP   EN FAKAFDTAN+IVTLRFIDPLWK+K+FLN GSE 
Sbjct: 169 RMTLDSICKVGFGVEIGTLAPELPENHFAKAFDTANIIVTLRFIDPLWKMKKFLNIGSEA 228

Query: 82  VLDKSIKIIDDFTYSVIATRKKEIQEAQTTSKDAN 116
           +L KSIK+++DFTYSVI  RK E+ EAQ +  + N
Sbjct: 229 LLGKSIKVVNDFTYSVIRRRKAELLEAQISPTNNN 263

[Close]

BLAST of Bhi06G000757 vs. TAIR 10
Match: AT2G44890.1 (cytochrome P450, family 704, subfamily A, polypeptide 1 )

HSP 1 Score: 86.7 bits (213), Expect = 1.4e-17
Identity = 50/90 (55.56%), Postives = 58/90 (64.44%), Query Frame = 0


Query: 24  TLDSICKVGFGVEIGSLAPNFLE-NSFAKAFDTANMIVTLRFIDPLWKIKRFLNWGSEV- 83
           TLDSI KVGFGVE+G L     E   F KAFD  N   + R  DP WK+K FLN GSE  
Sbjct: 175 TLDSIFKVGFGVELGCLDGFSKEGEEFMKAFDEGNGATSSRVTDPFWKLKCFLNIGSESR 234

Query: 84  LDKSIKIIDDFTYSVIATRKKEIQEAQTTS 112
           L KSI IID F YS+I T++KE+ + Q TS
Sbjct: 235 LKKSIAIIDKFVYSLITTKRKELSKEQNTS 264

[Close]

BLAST of Bhi06G000757 vs. TAIR 10
Match: AT2G44890.2 (cytochrome P450, family 704, subfamily A, polypeptide 1 )

HSP 1 Score: 85.5 bits (210), Expect = 3.0e-17
Identity = 49/89 (55.06%), Postives = 57/89 (64.04%), Query Frame = 0


Query: 24  TLDSICKVGFGVEIGSLAPNFLE-NSFAKAFDTANMIVTLRFIDPLWKIKRFLNWGSEV- 83
           TLDSI KVGFGVE+G L     E   F KAFD  N   + R  DP WK+K FLN GSE  
Sbjct: 181 TLDSIFKVGFGVELGCLDGFSKEGEEFMKAFDEGNGATSSRVTDPFWKLKCFLNIGSESR 240

Query: 84  LDKSIKIIDDFTYSVIATRKKEIQEAQTT 111
           L KSI IID F YS+I T++KE+ + Q T
Sbjct: 241 LKKSIAIIDKFVYSLITTKRKELSKEQNT 269

[Close]

BLAST of Bhi06G000757 vs. TAIR 10
Match: AT2G45510.1 (cytochrome P450, family 704, subfamily A, polypeptide 2 )

HSP 1 Score: 83.6 bits (205), Expect = 1.2e-16
Identity = 47/89 (52.81%), Postives = 58/89 (65.17%), Query Frame = 0


Query: 24  TLDSICKVGFGVEIGSLAPNFLE-NSFAKAFDTANMIVTLRFIDPLWKIKRFLNWGSE-V 83
           TLDSI KVGFGVE+  L     E   F +AFD  N+  + RFIDPLWK+K F N GS+  
Sbjct: 181 TLDSIFKVGFGVELKCLDGFSKEGQEFMEAFDEGNVATSSRFIDPLWKLKWFFNIGSQSK 240

Query: 84  LDKSIKIIDDFTYSVIATRKKEIQEAQTT 111
           L KSI  ID F YS+I T++KE+ + Q T
Sbjct: 241 LKKSIATIDKFVYSLITTKRKELAKEQNT 269

[Close]

BLAST of Bhi06G000757 vs. TAIR 10
Match: AT5G08250.1 (Cytochrome P450 superfamily protein )

HSP 1 Score: 75.1 bits (183), Expect = 4.1e-14
Identity = 39/86 (45.35%), Postives = 55/86 (63.95%), Query Frame = 0


Query: 22  QMTLDSICKVGFGVEIGSLAPNFLENSFAKAFDTANMIVTLRFIDP--LWKIKRFLNWGS 81
           ++T D++C + FGV+ G L+P   E  FAKAF+ A     +RF+ P  +WK+ R LN G+
Sbjct: 144 RLTFDNVCMIAFGVDPGCLSPKLPEIPFAKAFEDATEATVVRFVMPKFVWKLMRSLNLGT 203

Query: 82  E-VLDKSIKIIDDFTYSVIATRKKEI 105
           E  L +SI  +DDF   VI TRKKE+
Sbjct: 204 EKKLKESINGVDDFAEEVIRTRKKEM 229

[Close]

BLAST of Bhi06G000757 vs. ExPASy Swiss-Prot
Match: Q9C788 (Cytochrome P450 704B1 OS=Arabidopsis thaliana OX=3702 GN=CYP704B1 PE=1 SV=1)

HSP 1 Score: 134.8 bits (338), Expect = 6.2e-31
Identity = 68/95 (71.58%), Postives = 80/95 (84.21%), Query Frame = 0


Query: 22  QMTLDSICKVGFGVEIGSLAPNFLENSFAKAFDTANMIVTLRFIDPLWKIKRFLNWGSE- 81
           +MTLDSICKVGFGVEIG+LAP   EN FAKAFDTAN+IVTLRFIDPLWK+K+FLN GSE 
Sbjct: 169 RMTLDSICKVGFGVEIGTLAPELPENHFAKAFDTANIIVTLRFIDPLWKMKKFLNIGSEA 228

Query: 82  VLDKSIKIIDDFTYSVIATRKKEIQEAQTTSKDAN 116
           +L KSIK+++DFTYSVI  RK E+ EAQ +  + N
Sbjct: 229 LLGKSIKVVNDFTYSVIRRRKAELLEAQISPTNNN 263

[Close]

BLAST of Bhi06G000757 vs. ExPASy Swiss-Prot
Match: Q50EK3 (Cytochrome P450 704C1 OS=Pinus taeda OX=3352 GN=CYP704C1 PE=2 SV=1)

HSP 1 Score: 78.6 bits (192), Expect = 5.2e-14
Identity = 44/91 (48.35%), Postives = 62/91 (68.13%), Query Frame = 0


Query: 24  TLDSICKVGFGVEIGSLAPNFLEN----SFAKAFDTANMIVTLR-FIDPLWKIKRFLNWG 83
           +LDSICKV FG++I SL+ +  E+    SFAKAFD AN +V  R  +   WK++RF N G
Sbjct: 177 SLDSICKVVFGIDINSLSSSKAESGPEASFAKAFDVANAMVFHRHMVGSFWKVQRFFNVG 236

Query: 84  SE-VLDKSIKIIDDFTYSVIATRKKEIQEAQ 109
           SE +L  +IK++DDF Y VI  R++E+  A+
Sbjct: 237 SEAILRDNIKMVDDFLYKVIHFRRQEMFSAE 267

[Close]

BLAST of Bhi06G000757 vs. ExPASy Swiss-Prot
Match: Q9LMM1 (Cytochrome P450 86A4 OS=Arabidopsis thaliana OX=3702 GN=CYP86A4 PE=1 SV=1)

HSP 1 Score: 70.1 bits (170), Expect = 1.9e-11
Identity = 38/90 (42.22%), Postives = 55/90 (61.11%), Query Frame = 0


Query: 22  QMTLDSICKVGFGVEIGSLAPNFLENSFAKAFDTANMIVTLRFIDP--LWKIKRFLNWGS 81
           ++T D+IC + FG +  + AP   EN FA AFD A      RFI P  +WK+K++L  G 
Sbjct: 181 RLTFDNICGLAFGKDTRTCAPGLPENGFASAFDRATEASLQRFIIPKFMWKLKKWLGLGL 240

Query: 82  EV-LDKSIKIIDDFTYSVIATRKKEIQEAQ 109
           EV L +S+  ID++  +VI TRK+E+   Q
Sbjct: 241 EVSLSRSLGEIDEYLAAVINTRKQELMSQQ 270

[Close]

BLAST of Bhi06G000757 vs. ExPASy Swiss-Prot
Match: B3RFJ6 (Cytochrome P450 86A22 OS=Petunia hybrida OX=4102 GN=CYP86A22 PE=1 SV=1)

HSP 1 Score: 67.4 bits (163), Expect = 1.2e-10
Identity = 37/90 (41.11%), Postives = 55/90 (61.11%), Query Frame = 0


Query: 22  QMTLDSICKVGFGVEIGSLAPNFLENSFAKAFDTANMIVTLRFIDP--LWKIKRFLNWGS 81
           ++T D+IC + FG +  +L+P   EN+FA +FD A      RFI P  +WK+K+ L  G 
Sbjct: 183 RLTFDNICGLAFGKDPETLSPELPENNFATSFDRATEATLHRFIMPEFVWKLKKMLGLGL 242

Query: 82  EV-LDKSIKIIDDFTYSVIATRKKEIQEAQ 109
           EV L+ S+K +D++   VI TRK E+   Q
Sbjct: 243 EVSLNNSLKQVDNYMTDVINTRKLELLNHQ 272

[Close]

BLAST of Bhi06G000757 vs. ExPASy Swiss-Prot
Match: O23066 (Cytochrome P450 86A2 OS=Arabidopsis thaliana OX=3702 GN=CYP86A2 PE=1 SV=1)

HSP 1 Score: 66.2 bits (160), Expect = 2.7e-10
Identity = 37/86 (43.02%), Postives = 53/86 (61.63%), Query Frame = 0


Query: 22  QMTLDSICKVGFGVEIGSLAPNFLENSFAKAFDTANMIVTLRFIDP--LWKIKRFLNWGS 81
           ++T D+IC + FG +  + AP   EN FA AFD A      RFI P  LW++K++L  G 
Sbjct: 181 RLTFDNICGLAFGKDTRTCAPGLPENGFASAFDRATEASLQRFILPEFLWRLKKWLGLGL 240

Query: 82  EV-LDKSIKIIDDFTYSVIATRKKEI 105
           EV L +S+  ID +  +VI TRK+E+
Sbjct: 241 EVSLSRSLGEIDGYLDAVINTRKQEL 266

[Close]

BLAST of Bhi06G000757 vs. ExPASy TrEMBL
Match: A0A5D3BJX9 (Cytochrome P450 704B1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold316G00130 PE=3 SV=1)

HSP 1 Score: 162.9 bits (411), Expect = 7.8e-37
Identity = 84/95 (88.42%), Postives = 87/95 (91.58%), Query Frame = 0


Query: 22  QMTLDSICKVGFGVEIGSLAPNFLENSFAKAFDTANMIVTLRFIDPLWKIKRFLNWGSE- 81
           +MTLDSICKVGFGVEIGSLAPN  EN FAKAFDTANMIVTLRFIDPLWKIKRFLN GSE 
Sbjct: 158 RMTLDSICKVGFGVEIGSLAPNLPENKFAKAFDTANMIVTLRFIDPLWKIKRFLNLGSEA 217

Query: 82  VLDKSIKIIDDFTYSVIATRKKEIQEAQTTSKDAN 116
           +LDKSIKIIDDFTYSVIATRKKEIQEAQTT  + N
Sbjct: 218 LLDKSIKIIDDFTYSVIATRKKEIQEAQTTPTEPN 252

[Close]

BLAST of Bhi06G000757 vs. ExPASy TrEMBL
Match: A0A1S3B4S2 (cytochrome P450 704B1 OS=Cucumis melo OX=3656 GN=LOC103485770 PE=3 SV=1)

HSP 1 Score: 162.9 bits (411), Expect = 7.8e-37
Identity = 84/95 (88.42%), Postives = 87/95 (91.58%), Query Frame = 0


Query: 22  QMTLDSICKVGFGVEIGSLAPNFLENSFAKAFDTANMIVTLRFIDPLWKIKRFLNWGSE- 81
           +MTLDSICKVGFGVEIGSLAPN  EN FAKAFDTANMIVTLRFIDPLWKIKRFLN GSE 
Sbjct: 205 RMTLDSICKVGFGVEIGSLAPNLPENKFAKAFDTANMIVTLRFIDPLWKIKRFLNLGSEA 264

Query: 82  VLDKSIKIIDDFTYSVIATRKKEIQEAQTTSKDAN 116
           +LDKSIKIIDDFTYSVIATRKKEIQEAQTT  + N
Sbjct: 265 LLDKSIKIIDDFTYSVIATRKKEIQEAQTTPTEPN 299

[Close]

BLAST of Bhi06G000757 vs. ExPASy TrEMBL
Match: A0A0A0LN46 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G108690 PE=3 SV=1)

HSP 1 Score: 162.5 bits (410), Expect = 1.0e-36
Identity = 84/95 (88.42%), Postives = 86/95 (90.53%), Query Frame = 0


Query: 22  QMTLDSICKVGFGVEIGSLAPNFLENSFAKAFDTANMIVTLRFIDPLWKIKRFLNWGSE- 81
           +MTLDSICKVGFGVEIGSLAPN  EN FAKAFDTANMIVTLRFIDPLWKIKRFLN GSE 
Sbjct: 206 RMTLDSICKVGFGVEIGSLAPNLPENKFAKAFDTANMIVTLRFIDPLWKIKRFLNLGSEA 265

Query: 82  VLDKSIKIIDDFTYSVIATRKKEIQEAQTTSKDAN 116
           +LDKSIK IDDFTYSVIATRKKEIQEAQTT  D N
Sbjct: 266 LLDKSIKTIDDFTYSVIATRKKEIQEAQTTHTDPN 300

[Close]

BLAST of Bhi06G000757 vs. ExPASy TrEMBL
Match: A0A6J1DQX4 (cytochrome P450 704B1 OS=Momordica charantia OX=3673 GN=LOC111023497 PE=3 SV=1)

HSP 1 Score: 157.5 bits (397), Expect = 3.3e-35
Identity = 80/93 (86.02%), Postives = 86/93 (92.47%), Query Frame = 0


Query: 22  QMTLDSICKVGFGVEIGSLAPNFLENSFAKAFDTANMIVTLRFIDPLWKIKRFLNWGSE- 81
           +MTLDSICKVGFGVEIGSLAPN  EN FA+AFDTAN+IVTLRFIDPLWKIKRFLN GSE 
Sbjct: 185 RMTLDSICKVGFGVEIGSLAPNLPENCFAQAFDTANIIVTLRFIDPLWKIKRFLNLGSEA 244

Query: 82  VLDKSIKIIDDFTYSVIATRKKEIQEAQTTSKD 114
           +LDKSIKIIDDFTYSVIATRKKEI+EA+TT  D
Sbjct: 245 LLDKSIKIIDDFTYSVIATRKKEIEEARTTGND 277

[Close]

BLAST of Bhi06G000757 vs. ExPASy TrEMBL
Match: A0A6J1ICQ4 (cytochrome P450 704B1 OS=Cucurbita maxima OX=3661 GN=LOC111471352 PE=3 SV=1)

HSP 1 Score: 151.8 bits (382), Expect = 1.8e-33
Identity = 77/90 (85.56%), Postives = 81/90 (90.00%), Query Frame = 0


Query: 22  QMTLDSICKVGFGVEIGSLAPNFLENSFAKAFDTANMIVTLRFIDPLWKIKRFLNWGSE- 81
           +MTLDSICKVGFGVEIGSLAPN  EN FAKAFDTANMIVTLRFIDP WKIKRFLN GSE 
Sbjct: 179 RMTLDSICKVGFGVEIGSLAPNLPENGFAKAFDTANMIVTLRFIDPFWKIKRFLNMGSEA 238

Query: 82  VLDKSIKIIDDFTYSVIATRKKEIQEAQTT 111
           +L KSIK+ID FTYSVIATRKKEIQ+A TT
Sbjct: 239 LLQKSIKVIDHFTYSVIATRKKEIQQAPTT 268

The following BLAST results are available for this feature:

BLAST of Bhi06G000757 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G69500.1	4.4e-32	71.58	cytochrome P450, family 704, subfamily B, polypeptide 1	[more]
AT2G44890.1	1.4e-17	55.56	cytochrome P450, family 704, subfamily A, polypeptide 1	[more]
AT2G44890.2	3.0e-17	55.06	cytochrome P450, family 704, subfamily A, polypeptide 1	[more]
AT2G45510.1	1.2e-16	52.81	cytochrome P450, family 704, subfamily A, polypeptide 2	[more]
AT5G08250.1	4.1e-14	45.35	Cytochrome P450 superfamily protein	[more]

BLAST of Bhi06G000757 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9C788	6.2e-31	71.58	Cytochrome P450 704B1 OS=Arabidopsis thaliana OX=3702 GN=CYP704B1 PE=1 SV=1	[more]
Q50EK3	5.2e-14	48.35	Cytochrome P450 704C1 OS=Pinus taeda OX=3352 GN=CYP704C1 PE=2 SV=1	[more]
Q9LMM1	1.9e-11	42.22	Cytochrome P450 86A4 OS=Arabidopsis thaliana OX=3702 GN=CYP86A4 PE=1 SV=1	[more]
B3RFJ6	1.2e-10	41.11	Cytochrome P450 86A22 OS=Petunia hybrida OX=4102 GN=CYP86A22 PE=1 SV=1	[more]
O23066	2.7e-10	43.02	Cytochrome P450 86A2 OS=Arabidopsis thaliana OX=3702 GN=CYP86A2 PE=1 SV=1	[more]

BLAST of Bhi06G000757 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A5D3BJX9	7.8e-37	88.42	Cytochrome P450 704B1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold31...	[more]
A0A1S3B4S2	7.8e-37	88.42	cytochrome P450 704B1 OS=Cucumis melo OX=3656 GN=LOC103485770 PE=3 SV=1	[more]
A0A0A0LN46	1.0e-36	88.42	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G108690 PE=3 SV=1	[more]
A0A6J1DQX4	3.3e-35	86.02	cytochrome P450 704B1 OS=Momordica charantia OX=3673 GN=LOC111023497 PE=3 SV=1	[more]
A0A6J1ICQ4	1.8e-33	85.56	cytochrome P450 704B1 OS=Cucurbita maxima OX=3661 GN=LOC111471352 PE=3 SV=1	[more]

InterPro

Analysis Name: InterPro Annotations of Wax gourd (B227) v1
Date Performed: 2021-10-22


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001128	Cytochrome P450	PFAM	PF00067	p450	coord: 14..111 e-value: 1.7E-5 score: 23.8
IPR036396	Cytochrome P450 superfamily	GENE3D	1.10.630.10	Cytochrome P450	coord: 4..114 e-value: 1.7E-9 score: 38.8
IPR036396	Cytochrome P450 superfamily	SUPERFAMILY	48264	Cytochrome P450	coord: 18..107
None	No IPR available	PANTHER	PTHR24296:SF8	CYTOCHROME P450 704B1	coord: 19..109
None	No IPR available	PANTHER	PTHR24296	CYTOCHROME P450	coord: 19..109

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Bhi06M000757	Bhi06M000757	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
cellular_component	GO:0016021	integral component of membrane
molecular_function	GO:0020037	heme binding
molecular_function	GO:0005506	iron ion binding
molecular_function	GO:0004497	monooxygenase activity
molecular_function	GO:0016705	oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen