Bhi06G000662 (gene) Wax gourd (B227) v1

Overview


Name	Bhi06G000662
Type	gene
Organism	Benincasa hispida (Wax gourd (B227) v1)
Description	Cystatin
Location	chr6: 18362572 .. 18364543 (+)
RNA-Seq Expression	Bhi06G000662
Synteny	Bhi06G000662

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRexonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ACCACTCTTACGAAAAAGGTATATATCTATCTATCAAAAGAGGTAATTAGCGATGAGTTCCGAAGATATTGATCTAATTCCTAATGTTGATCCAGTGCCAATTAAGGATATTAATAGCGAGGAGATACAAGGATATGGAAGCTTTGCAGTGCGTGAGCATAATGAAACAACAGGAGAAAAACTGGAGTTCGAGCGCGTGATAAACGGATTGGAACAAGGAAAAAGGTCTATTCGAATAGTTTTCACTCTTGTGCTGGTGGCTAAAAACTCCTGTGGAATTAATTGGACTTATACAACTAAGGTGTTTTTTAATCAACGGGCAGCAGTACGTCTCTTAAATTTTGAATCTGTACTTCCAGAGAATAAAAGCTAAAGTTGAATTGCTATGTTTACTTTAACAATATCCATGCAAAAATCTATGAAATAAAATAAATGAATAAAATAATTATACTTATCTTTATTTCTAATCTCTATCTACTGATATATGTGGTATTTTTCTCTTCACTTTTTATTTCATTCTCTCTCATAGTATTAAATTTCCATCTACCTATCGATATTTTCATCGATATTTCCATATTTTCATGAGTTCAATATGGATATGAAGGGTGTATCGATATTTTCTTTGTAATGTAGAAAAATCCAATAAACATAAAAATAAAAATAAAATATCACTAATGTAAATATAATATAAATAATAGATATGTTAGTTTGTTTAAAAATCATAAAATATTTATTAACTAAATTTTTTGGTGTTATAACTCTTCTAGAAATATTCATCGAAATCAATATTTTCATCAAAATTTTAATAAAATTAAGACTACGATATTTGCGTTGAAATCAACCTTTCAAACCTTTATACCATATTGATATTATTTTAGGTTTAAATACTACTTTGGTACATGTACTTTAAAAAGGTTCATTTTATACCTTATACTTTAAGAAAAGGTTCAATTTGGTCGTTGGGGGTCAGCATGTTGTTAAAAAACTAATGGATTCATTAGATGACATTTTTTATGAGATGATTAGGTGCCACATAGTTAGTTTAAGTTCCAAATAATTCAAGTCGCTAGGGTCGGACCAATTAAATCCCAACTAATTTTGCGTTCATTAGGGTTTGTTGTTTCGGTTCTTTTCCTATTGTTAAATATTATTTTCGATCAATTGGTCCCTATTACTATACATCCTAATTTATTGATTATATGGTTGGTCTTTTAATGTGTTTTCTGTCAAAAAAAAAAAAAAAGTAATACATGGTCCCTACTATTTTGTCATTTTTGCAATTTTTAATTTTTAATTTTTTACCATGTGAATGGACTCCTTGTGTTTCATTTAAGTAATATCATTCAATTGTAGAGATGTCAACCCTCAACATAAAGATTTATCACGGTTGAATGTTCATATGTGATCCTTATACGAAATATGAGAATGGAAAGGTGGATATAGTCCATCATTTTGACCTAAATATATGGTCCTATTTTTAAGCCGTAGATATTGTGAAAAACCTTCGACATACTAGTTGGGAGAAATTGGGGAGTGGGGAATCTTACAAATGATGTTGGAGGATATCGTGTATTAAAGAGTGATGAAGATGTTATGGACTTCATAAGACTCATCGATGATAAGAAGAGTTTTGAGTTGTATTTGGAGCATGAGCCTTTTAATGATAATAACGATAACTTTTTTGAAATTATTCCAAACATGTAACCAATTCTATCAATGCTCATTACCTATATTGTGATAATAGTGGTGCTGCATCGAACTCCAAGGAGCCTCGAAACCACAAATGAGGAAAACACATCGAGCGAGAGTATCATCTCATTTAAAAGATTGTGCAACACGAAGATGTGATCGCCACGAAGATCGCTTCGGAGAAAA

mRNA sequence

ACCACTCTTACGAAAAAGGTATATATCTATCTATCAAAAGAGGTAATTAGCGATGAGTTCCGAAGATATTGATCTAATTCCTAATGTTGATCCAGTGCCAATTAAGGATATTAATAGCGAGGAGATACAAGGATATGGAAGCTTTGCAGTGCGTGAGCATAATGAAACAACAGGAGAAAAACTGGAGTTCGAGCGCGTGATAAACGGATTGGAACAAGGAAAAAGGTCTATTCGAATAGTTTTCACTCTTGTGCTGGTGGCTAAAAACTCCTGTGGAATTAATTGGACTTATACAACTAAGGTGTTTTTTAATCAACGGGCAGCATGGTGCTGCATCGAACTCCAAGGAGCCTCGAAACCACAAATGAGGAAAACACATCGAGCGAGAGTATCATCTCATTTAAAAGATTGTGCAACACGAAGATGTGATCGCCACGAAGATCGCTTCGGAGAAAA

Coding sequence (CDS)

ATGAGTTCCGAAGATATTGATCTAATTCCTAATGTTGATCCAGTGCCAATTAAGGATATTAATAGCGAGGAGATACAAGGATATGGAAGCTTTGCAGTGCGTGAGCATAATGAAACAACAGGAGAAAAACTGGAGTTCGAGCGCGTGATAAACGGATTGGAACAAGGAAAAAGGTCTATTCGAATAGTTTTCACTCTTGTGCTGGTGGCTAAAAACTCCTGTGGAATTAATTGGACTTATACAACTAAGGTGTTTTTTAATCAACGGGCAGCATGGTGCTGCATCGAACTCCAAGGAGCCTCGAAACCACAAATGAGGAAAACACATCGAGCGAGAGTATCATCTCATTTAAAAGATTGTGCAACACGAAGATGTGATCGCCACGAAGATCGCTTCGGAGAAAA

Protein sequence

MSSEDIDLIPNVDPVPIKDINSEEIQGYGSFAVREHNETTGEKLEFERVINGLEQGKRSIRIVFTLVLVAKNSCGINWTYTTKVFFNQRAAWCCIELQGASKPQMRKTHRARVSSHLKDCATRRCDRHEDRFGE

Homology

[Close]

BLAST of Bhi06G000662 vs. ExPASy TrEMBL
Match: A0A5A7TFU6 (Cystatin OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold98G00010 PE=4 SV=1)

HSP 1 Score: 129.0 bits (323), Expect = 1.5e-26
Identity = 65/98 (66.33%), Postives = 76/98 (77.55%), Query Frame = 0


Query: 1  MSSEDIDLIPNVDPVPIKDINSEEIQGYGSFAVREHNETTGEKLEFERVINGLEQGKRSI 60
          MSSED+    N+DPVPIKD++SEEIQG G FAVREHNE  GE LEF +VING EQG+R +
Sbjct: 1  MSSEDLS---NLDPVPIKDVDSEEIQGLGEFAVREHNEEKGENLEFVKVINGFEQGRRGV 60

Query: 61 RIVFTLVLVAKNSCGINWTYTTKVFFNQRAAWCCIELQ 99
          RI+FT+VL AKN  GI WTY  KVFFNQRAA   +E +
Sbjct: 61 RIIFTIVLSAKNCNGIIWTYKAKVFFNQRAAVRLLEFE 95

[Close]

BLAST of Bhi06G000662 vs. ExPASy TrEMBL
Match: A0A0A0KRZ8 (Cystatin domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_5G487690 PE=4 SV=1)

HSP 1 Score: 127.1 bits (318), Expect = 5.5e-26
Identity = 68/99 (68.69%), Postives = 76/99 (76.77%), Query Frame = 0


Query: 1  MSSEDIDLIPNVDP-VPIKDINSEEIQGYGSFAVREHNETTGEKLEFERVINGLEQGKRS 60
          MSSEDI  IP VDP VPIKD++SEEI+G+G FAV EHNE TGE LEF +VINGLEQG+R 
Sbjct: 1  MSSEDIS-IPKVDPAVPIKDVDSEEIRGFGEFAVSEHNEATGENLEFVKVINGLEQGRRG 60

Query: 61 IRIVFTLVLVAKNSCGINWTYTTKVFFNQRAAWCCIELQ 99
           RI FTLVL AKNS GI WTY  KVF NQRA    +E +
Sbjct: 61 FRIDFTLVLSAKNSIGIIWTYKVKVFLNQRAGVRLLEFE 98

[Close]

BLAST of Bhi06G000662 vs. ExPASy TrEMBL
Match: A0A5D3C5B1 (Cystatin OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold98G00040 PE=4 SV=1)

HSP 1 Score: 126.7 bits (317), Expect = 7.2e-26
Identity = 67/100 (67.00%), Postives = 81/100 (81.00%), Query Frame = 0


Query: 1  MSSEDIDLIPNVDP-VPIKDINSEEIQGYGSFAVREHNE-TTGEKLEFERVINGLEQGKR 60
          MSS D++L P V+P VPIKD++SEEIQG+G FAVR+HN+ +TGE LEF +VINGLEQG+R
Sbjct: 1  MSSPDVEL-PCVEPQVPIKDVDSEEIQGFGRFAVRKHNKISTGENLEFVKVINGLEQGRR 60

Query: 61 SIRIVFTLVLVAKNSCGINWTYTTKVFFNQRAAWCCIELQ 99
            RIV+TLVL AKNSCGI WTY  KVFFN RAA   +E +
Sbjct: 61 GFRIVYTLVLSAKNSCGIIWTYKAKVFFNNRAAVLLLEFE 99

[Close]

BLAST of Bhi06G000662 vs. ExPASy TrEMBL
Match: A0A0A0KNL2 (Cystatin domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_5G487700 PE=4 SV=1)

HSP 1 Score: 112.8 bits (281), Expect = 1.1e-21
Identity = 57/87 (65.52%), Postives = 67/87 (77.01%), Query Frame = 0


Query: 6  IDLIPNVDPVPIKDINSEEIQGYGSFAVREHNET-TGEKLEFERVINGLEQGKRSIRIVF 65
          +  +P+ D VPIKD++SEEIQG G  AVR HNET TGE LEF +VINGLE+G+R  +IV+
Sbjct: 12 VSKLPDYDTVPIKDVDSEEIQGIGGMAVRMHNETSTGENLEFVKVINGLEKGRRGFQIVY 71

Query: 66 TLVLVAKNSCGINWTYTTKVFFNQRAA 92
          TLVL AKNS GI WTY  KV FN RAA
Sbjct: 72 TLVLSAKNSLGIVWTYKAKVIFNNRAA 98

[Close]

BLAST of Bhi06G000662 vs. ExPASy TrEMBL
Match: A0A5D3DF67 (Cystatin OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold26872G00010 PE=4 SV=1)

HSP 1 Score: 82.0 bits (201), Expect = 2.0e-12
Identity = 42/68 (61.76%), Postives = 51/68 (75.00%), Query Frame = 0


Query: 1  MSSEDIDLIPNVDPVPIKDINSEEIQGYGSFAVREHNETTGEKLEFERVINGLEQGKRSI 60
          MS ED+    N+DPVP KD++SEEIQG+G+FAVREHNE  GE LEF +VING EQG+R  
Sbjct: 1  MSFEDLS---NLDPVPFKDVDSEEIQGFGAFAVREHNEEKGENLEFVKVINGFEQGRRVR 60

Query: 61 RIVFTLVL 69
           + F  VL
Sbjct: 61 LLEFEAVL 65

The following BLAST results are available for this feature:

BLAST of Bhi06G000662 vs.


Match Name	E-value	Identity	Description

BLAST of Bhi06G000662 vs.


Match Name	E-value	Identity	Description

BLAST of Bhi06G000662 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A5A7TFU6	1.5e-26	66.33	Cystatin OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold98G00010 PE=4 S...	[more]
A0A0A0KRZ8	5.5e-26	68.69	Cystatin domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_5G487690 PE...	[more]
A0A5D3C5B1	7.2e-26	67.00	Cystatin OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold98G00040 PE=4 S...	[more]
A0A0A0KNL2	1.1e-21	65.52	Cystatin domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_5G487700 PE...	[more]
A0A5D3DF67	2.0e-12	61.76	Cystatin OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold26872G00010 PE=...	[more]

InterPro

Analysis Name: InterPro Annotations of Wax gourd (B227) v1
Date Performed: 2021-10-22


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR000010	Cystatin domain	PFAM	PF16845	SQAPI	coord: 17..86 e-value: 6.8E-11 score: 42.4
None	No IPR available	GENE3D	3.10.450.10		coord: 11..109 e-value: 9.9E-13 score: 50.0
None	No IPR available	SUPERFAMILY	54403	Cystatin/monellin	coord: 17..86

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Bhi06M000662	Bhi06M000662	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0010951	negative regulation of endopeptidase activity
molecular_function	GO:0004869	cysteine-type endopeptidase inhibitor activity