Bhi06G000234 (gene) Wax gourd (B227) v1

BLAST of Bhi06G000234 vs. TAIR 10
Match: AT1G35420.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 291.2 bits (744), Expect = 9.8e-79
Identity = 134/242 (55.37%), Postives = 176/242 (72.73%), Query Frame = 0

Query: 73  DDDDDDAACELVNGMELSIGEQPHHSIPAYLFKAVKNNNGTAILLLSDLFAFQHSSIRDF 132
           DDD+++ ACELVNG E+S+       +  YL  AVKNNNGT +LLLSD+F FQ S+ RDF
Sbjct: 74  DDDEEEEACELVNGTEVSV-----DGVEGYLLTAVKNNNGTGLLLLSDVFGFQDSATRDF 133

Query: 133 AYRLACNGFNVLLPDLIHGDSWKKQSTK---------RDMQRIAEDIGTVTKWLVDEFSA 192
           AYR+ACNG+NVL+PDL  GD W K   K          D  RI +D  T TKW+V+EF+A
Sbjct: 134 AYRVACNGYNVLVPDLFRGDPWSKNRPKSEYEEWRRGHDPNRIRQDTTTFTKWMVEEFAA 193

Query: 193 AGLSRKLGIMGFGYGGGQVIEVLARDEGACFCIGASLYGTEVDPSLATKVKVPVLLISGD 252
           AG+S+KLG+MGF +GGG+V++VLA DE   F  G S YGT +D ++A  VKVPVL I+GD
Sbjct: 194 AGISKKLGVMGFCFGGGRVVDVLATDESGYFSTGVSFYGTRIDSAVAGDVKVPVLFIAGD 253

Query: 253 NDPLCPVSVMKDLEKRMGEGSKLVIFGGRGHDFVHNPRSPEEDEDAEQAFLMIRNWLHHG 306
            DPLC V  + ++E+++GEGSK+V++ GRGH FVH P +PE+D DAE+AF ++RNWLHH 
Sbjct: 254 RDPLCEVKGLYEIEEKIGEGSKVVVYEGRGHGFVHRPETPEDDRDAEEAFALMRNWLHHH 310

BLAST of Bhi06G000234 vs. TAIR 10
Match: AT1G35420.2 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 291.2 bits (744), Expect = 9.8e-79
Identity = 134/242 (55.37%), Postives = 176/242 (72.73%), Query Frame = 0

Query: 73  DDDDDDAACELVNGMELSIGEQPHHSIPAYLFKAVKNNNGTAILLLSDLFAFQHSSIRDF 132
           DDD+++ ACELVNG E+S+       +  YL  AVKNNNGT +LLLSD+F FQ S+ RDF
Sbjct: 79  DDDEEEEACELVNGTEVSV-----DGVEGYLLTAVKNNNGTGLLLLSDVFGFQDSATRDF 138

Query: 133 AYRLACNGFNVLLPDLIHGDSWKKQSTK---------RDMQRIAEDIGTVTKWLVDEFSA 192
           AYR+ACNG+NVL+PDL  GD W K   K          D  RI +D  T TKW+V+EF+A
Sbjct: 139 AYRVACNGYNVLVPDLFRGDPWSKNRPKSEYEEWRRGHDPNRIRQDTTTFTKWMVEEFAA 198

Query: 193 AGLSRKLGIMGFGYGGGQVIEVLARDEGACFCIGASLYGTEVDPSLATKVKVPVLLISGD 252
           AG+S+KLG+MGF +GGG+V++VLA DE   F  G S YGT +D ++A  VKVPVL I+GD
Sbjct: 199 AGISKKLGVMGFCFGGGRVVDVLATDESGYFSTGVSFYGTRIDSAVAGDVKVPVLFIAGD 258

Query: 253 NDPLCPVSVMKDLEKRMGEGSKLVIFGGRGHDFVHNPRSPEEDEDAEQAFLMIRNWLHHG 306
            DPLC V  + ++E+++GEGSK+V++ GRGH FVH P +PE+D DAE+AF ++RNWLHH 
Sbjct: 259 RDPLCEVKGLYEIEEKIGEGSKVVVYEGRGHGFVHRPETPEDDRDAEEAFALMRNWLHHH 315

BLAST of Bhi06G000234 vs. TAIR 10
Match: AT3G23600.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 58.2 bits (139), Expect = 1.4e-08
Identity = 47/196 (23.98%), Postives = 93/196 (47.45%), Query Frame = 0

Query: 115 ILLLSDLFAFQHSSIRDFAYRLACNGFNVLLPDLIHGDSWKKQSTKRDMQRIAEDIG--- 174
           +LL+SD+F F+  ++R  A ++A +GF V++PD   GD +   +  R +    +D G   
Sbjct: 42  VLLISDIFGFEAPNLRALADKVAASGFYVVVPDYFGGDPYNPSNQDRPIPVWIKDHGCDK 101

Query: 175 --TVTKWLVDEFSAAGLSRKLGIMGFGYGGGQVIEVLARDEGACFCIGASLYGTEVDPSL 234
               TK +++     G++  +G  G  +G   V+E L+++E         L+ + V+   
Sbjct: 102 GFENTKPVLETIKNKGIT-AIGAAGMCWGAKVVVE-LSKEE--LIQAAVLLHPSFVNVDD 161

Query: 235 ATKVKVPVLLISGDNDPLCPVSVMKDLEKRMGE----GSKLVIFGGRGHDFV--HNPRSP 294
               K P+ ++  + D + P +++K  E+ +       S + I     H +   +N   P
Sbjct: 162 IKGGKAPIAILGAEIDQMSPPALLKQFEEILSSKPEVNSYVKIHPKVSHGWTVRYNIDEP 221

Query: 295 EEDEDAEQAFLMIRNW 300
           E  + AE+A   + +W
Sbjct: 222 EAVKAAEEAHKEMLDW 233

BLAST of Bhi06G000234 vs. TAIR 10
Match: AT3G23600.2 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 54.7 bits (130), Expect = 1.5e-07
Identity = 47/196 (23.98%), Postives = 93/196 (47.45%), Query Frame = 0

Query: 115 ILLLSDLFAFQHSSIRDFAYRLACNGFNVLLPDLIHGDSWKKQSTKRDMQRIAEDIG--- 174
           +LL+SD+F F+  ++R  A ++A +GF V++PD   GD +   +  R +    +D G   
Sbjct: 42  VLLISDIFGFEAPNLRALADKVAASGFYVVVPDYFGGDPYNPSNQDRPIPVWIKDHGCDK 101

Query: 175 --TVTKWLVDEFSAAGLSRKLGIMGFGYGGGQVIEVLARDEGACFCIGASLYGTEVDPSL 234
               TK +++     G++  +G  G  +G   V+E L+++E         L+ + V+   
Sbjct: 102 GFENTKPVLETIKNKGIT-AIGAAGMCWG---VVE-LSKEE--LIQAAVLLHPSFVNVDD 161

Query: 235 ATKVKVPVLLISGDNDPLCPVSVMKDLEKRMGE----GSKLVIFGGRGHDFV--HNPRSP 294
               K P+ ++  + D + P +++K  E+ +       S + I     H +   +N   P
Sbjct: 162 IKGGKAPIAILGAEIDQMSPPALLKQFEEILSSKPEVNSYVKIHPKVSHGWTVRYNIDEP 221

Query: 295 EEDEDAEQAFLMIRNW 300
           E  + AE+A   + +W
Sbjct: 222 EAVKAAEEAHKEMLDW 230

BLAST of Bhi06G000234 vs. TAIR 10
Match: AT1G78210.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 43.9 bits (102), Expect = 2.7e-04
Identity = 24/64 (37.50%), Postives = 38/64 (59.38%), Query Frame = 0

Query: 232 KVKVPVLLISGDNDPLCPVSVMKDLEKRMGEGSKLVIFGGRGHDFVHNPRSPEEDEDAEQ 291
           K+K P L+I G++D + P+ + K LEK +G+  KLVI    GH F  N   P++     +
Sbjct: 241 KLKQPTLIIWGEHDQVFPLEMGKRLEKHVGDNGKLVIIKRTGHIF--NFEKPKKFIKLLK 300

Query: 292 AFLM 296
           +FL+
Sbjct: 301 SFLL 302

BLAST of Bhi06G000234 vs. ExPASy Swiss-Prot
Match: Q96DG6 (Carboxymethylenebutenolidase homolog OS=Homo sapiens OX=9606 GN=CMBL PE=1 SV=1)

HSP 1 Score: 80.9 bits (198), Expect = 2.8e-14
Identity = 60/220 (27.27%), Postives = 105/220 (47.73%), Query Frame = 0

Query: 99  IPAYLFKAVKNNNGTAILLLSDLFAFQHSSIRDFAYRLACNGFNVLLPDLIHG-DSWKKQ 158
           I AY+ K+   + G A++++ D+F +Q  + R  A  ++ NG+  ++PD   G + W   
Sbjct: 30  IKAYVTKS-PVDAGKAVIVIQDIFGWQLPNTRYIADMISGNGYTTIVPDFFVGQEPWDPS 89

Query: 159 S---------TKRDMQRIAEDIGTVTKWLVDEFSAAGLSRKLGIMGFGYGGGQVIEVLAR 218
                       R+ Q+I  +I  + K+L  +  A    +K+GI+GF +GG  V  ++ +
Sbjct: 90  GDWSIFPEWLKTRNAQKIDREISAILKYLKQQCHA----QKIGIVGFCWGGTAVHHLMMK 149

Query: 219 DEGACFCIGASLYGTEVDPSLATKVKVPVLLISGDNDPLCPVSVMKDLEKRMGEGSK--- 278
                F  G S+YG   D      +K P L I  +ND + P+  +  L +++ E  K   
Sbjct: 150 YSE--FRAGVSVYGIVKDSEDIYNLKNPTLFIFAENDVVIPLKDVSLLTQKLKEHCKVEY 209

Query: 279 -LVIFGGRGHDFVHNPR---SPEEDEDAEQAFLMIRNWLH 302
            +  F G+ H FVH  R   SP +    ++A   +  WL+
Sbjct: 210 QIKTFSGQTHGFVHRKREDCSPADKPYIDEARRNLIEWLN 242

BLAST of Bhi06G000234 vs. ExPASy Swiss-Prot
Match: Q5RBU3 (Carboxymethylenebutenolidase homolog OS=Pongo abelii OX=9601 GN=CMBL PE=2 SV=1)

HSP 1 Score: 80.1 bits (196), Expect = 4.8e-14
Identity = 60/220 (27.27%), Postives = 105/220 (47.73%), Query Frame = 0

Query: 99  IPAYLFKAVKNNNGTAILLLSDLFAFQHSSIRDFAYRLACNGFNVLLPDLIHG-DSWKKQ 158
           I AY+ K+   + G A++++ D+F +Q  + R  A  ++ NG+  ++PD   G + W   
Sbjct: 30  IKAYVTKS-PVDAGKAVIVIQDIFGWQLPNTRYMADMISGNGYTTIVPDFFVGQEPWDPS 89

Query: 159 S---------TKRDMQRIAEDIGTVTKWLVDEFSAAGLSRKLGIMGFGYGGGQVIEVLAR 218
                       R+ Q+I  +I  + K+L  +  A    +K+GI+GF +GG  V  ++ +
Sbjct: 90  GDWSIFPEWLKTRNAQKIDREISAILKYLKQQCHA----QKIGIVGFCWGGIAVHHLMMK 149

Query: 219 DEGACFCIGASLYGTEVDPSLATKVKVPVLLISGDNDPLCPVSVMKDLEKRMGEGSK--- 278
                F  G S+YG   D      +K P L I  +ND + P+  +  L +++ E  K   
Sbjct: 150 YSE--FRAGVSVYGIVKDSEDIYNLKNPTLFIFAENDVVIPLKDVSLLTQKLKEHCKVEY 209

Query: 279 -LVIFGGRGHDFVHNPR---SPEEDEDAEQAFLMIRNWLH 302
            +  F G+ H FVH  R   SP +    ++A   +  WL+
Sbjct: 210 QIKTFSGQTHGFVHRKREDCSPADKPYIDEARRNLIEWLN 242

BLAST of Bhi06G000234 vs. ExPASy Swiss-Prot
Match: Q8R1G2 (Carboxymethylenebutenolidase homolog OS=Mus musculus OX=10090 GN=Cmbl PE=1 SV=1)

HSP 1 Score: 78.2 bits (191), Expect = 1.8e-13
Identity = 63/245 (25.71%), Postives = 111/245 (45.31%), Query Frame = 0

Query: 81  CELVNGMELSIGEQPH----HSIPAYLFKAVKNNNGTAILLLSDLFAFQHSSIRDFAYRL 140
           C    G +L  G   H      I AY+ ++   + G A++++ D+F +Q  + R  A  +
Sbjct: 8   CPCDIGHKLEYGGMGHEVQVEHIKAYVTRS-PVDAGKAVIVVQDIFGWQLPNTRYMADMI 67

Query: 141 ACNGFNVLLPDLIHGD-------------SWKKQSTKRDMQRIAEDIGTVTKWLVDEFSA 200
           A NG+  ++PD   G              +W K    R+ +++  ++  V ++L  +  A
Sbjct: 68  ARNGYTTIVPDFFVGQEPWDPAGDWSTFPAWLK---SRNARKVNREVDAVLRYLRQQCHA 127

Query: 201 AGLSRKLGIMGFGYGGGQVIEVLARDEGACFCIGASLYGTEVDPSLATKVKVPVLLISGD 260
               +K+GI+GF +GG  V +V+          G S+YG   D      +K P L I  +
Sbjct: 128 ----QKIGIVGFCWGGVVVHQVMTAYPD--IRAGVSVYGIIRDSEDVYNLKNPTLFIFAE 187

Query: 261 NDPLCPVSVMKDLEKRMGE----GSKLVIFGGRGHDFVHNPR---SPEEDEDAEQAFLMI 302
           ND + P+  +  L +++ E      ++  F G+ H FVH  R   SP +    E+A   +
Sbjct: 188 NDTVIPLEQVSTLTQKLKEHCIVNYQVKTFSGQTHGFVHRKREDCSPADKPYIEEARRNL 242

BLAST of Bhi06G000234 vs. ExPASy Swiss-Prot
Match: Q7TP52 (Carboxymethylenebutenolidase homolog OS=Rattus norvegicus OX=10116 GN=Cmbl PE=2 SV=1)

HSP 1 Score: 75.5 bits (184), Expect = 1.2e-12
Identity = 55/207 (26.57%), Postives = 98/207 (47.34%), Query Frame = 0

Query: 112 GTAILLLSDLFAFQHSSIRDFAYRLACNGFNVLLPDLIHG----------DSWKKQSTKR 171
           G A++++ D+F +Q S+ R  A  +A NG+  ++PD   G           ++ +    R
Sbjct: 42  GKAVIVVQDIFGWQLSNTRYMADMIAGNGYTTIVPDFFVGQEPWDPAGDWSTFPEWLKSR 101

Query: 172 DMQRIAEDIGTVTKWLVDEFSAAGLSRKLGIMGFGYGGGQVIEVLARDEGACFCIGASLY 231
           + ++I  ++  V ++L  +  A    +K+GI+GF +GG  V  V+          G S+Y
Sbjct: 102 NARKINREVDAVLRYLKQQCHA----QKIGIVGFCWGGIVVHHVMTTYPEV--RAGVSVY 161

Query: 232 GTEVDPSLATKVKVPVLLISGDNDPLCPVSVMKDLEKRMGE----GSKLVIFGGRGHDFV 291
           G   D      +K P L I  +ND + P+  +  L +++ E      ++  F G+ H FV
Sbjct: 162 GIIRDSEDVYNLKNPTLFIFAENDAVIPLEQVSILIQKLKEHCIVNYQVKTFSGQTHGFV 221

Query: 292 HNPR---SPEEDEDAEQAFLMIRNWLH 302
           H  R   SP +    E+A   +  WL+
Sbjct: 222 HRKREDCSPADKPYIEEARRNLIEWLN 242

BLAST of Bhi06G000234 vs. ExPASy Swiss-Prot
Match: B8M9K0 (Hydrolase tropI OS=Talaromyces stipitatus (strain ATCC 10500 / CBS 375.48 / QM 6759 / NRRL 1006) OX=441959 GN=tropI PE=3 SV=1)

HSP 1 Score: 53.1 bits (126), Expect = 6.3e-06
Identity = 36/179 (20.11%), Postives = 83/179 (46.37%), Query Frame = 0

Query: 115 ILLLSDLFAFQHSSIRDFAYRLACNGFNVLLPDLIHGDS-------------WKKQS-TK 174
           +L+L+D+   + S+ +  A + A NG+ V++PDL  GD+             W+  +   
Sbjct: 46  VLILTDIVGHRFSNAQIIADQFAENGYFVMMPDLFLGDAVPLNKPGEFDMGKWRSGAYHP 105

Query: 175 RDMQRIAEDIGTVTKWLVDEFSAAGLSRKLGIMGFGYGGGQVIEVLARDEGACFCIGASL 234
           +    + E +  + +  + E  +    +K+G +G+ +GG  V+  L   +     +G + 
Sbjct: 106 QGKNHLPETVDPIVEVCLSEMRSKYQCKKIGAVGYCFGGKYVVRHLIPGK---MDVGYTA 165

Query: 235 YGTEVDPSLATKVKVPVLLISGDNDPLCPVS---VMKDLEKRMGEGSKLVIFGGRGHDF 277
           + + +D S    +K P+ + +   D + P     V +++ + +G   ++ ++ G  H F
Sbjct: 166 HPSHIDESELKGIKGPLAIAAAAKDNIFPAEKRHVSEEILQEVGFPYQINLYSGVSHGF 221

BLAST of Bhi06G000234 vs. ExPASy TrEMBL
Match: A0A0A0KT04 (DLH domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_5G652280 PE=4 SV=1)

HSP 1 Score: 503.8 bits (1296), Expect = 5.0e-139
Identity = 251/312 (80.45%), Postives = 269/312 (86.22%), Query Frame = 0

Query: 1   MGLASAGAQLISLPPTGSSSCRRRIGTPFQLPYFRFTSPLPFRKCGNRSDWKWKGKREKK 60
           MG ASAGAQLISLPPTGSSS  RR+G PFQ PYF+F  PLP RK  N   W+WK      
Sbjct: 1   MGFASAGAQLISLPPTGSSS--RRVGKPFQPPYFKFAYPLPSRKYSNMGVWRWK------ 60

Query: 61  GGKVCYSVEGVEDDDDDDAACELVNGMELSIGEQPHHSIPAYLFKAVKNNNGTAILLLSD 120
            GK+ YSVEG   DDDDD ACELVNGMELSIGEQPHHSIPAYLFKAVKNNNGTAILLLSD
Sbjct: 61  -GKLRYSVEGRMKDDDDDDACELVNGMELSIGEQPHHSIPAYLFKAVKNNNGTAILLLSD 120

Query: 121 LFAFQHSSIRDFAYRLACNGFNVLLPDLIHGDSWKK----QSTKRDMQRIAEDIGTVTKW 180
           LFAFQHSSIRDFAYRLAC GFNVLLPDL+H D W+K       K++MQRIAEDIGTVTKW
Sbjct: 121 LFAFQHSSIRDFAYRLACIGFNVLLPDLVHEDVWEKPPAEHCAKQEMQRIAEDIGTVTKW 180

Query: 181 LVDEFSAAGLSRKLGIMGFGYGGGQVIEVLARDEGACFCIGASLYGTEVDPSLATKVKVP 240
           L +EFSAAGLS KLGI+GFGYGGG+VI+VLARDEG CF IGAS+YGT+VDPSLATKVKVP
Sbjct: 181 LTEEFSAAGLSMKLGIIGFGYGGGRVIDVLARDEGTCFLIGASIYGTQVDPSLATKVKVP 240

Query: 241 VLLISGDNDPLCPVSVMKDLEKRMGEGSKLVIFGGRGHDFVHNPRSPEEDEDAEQAFLMI 300
           VLLISGDNDPLCPVSVMKDLEK +GEGSKLVIFGGRGH FVHNP SPEED+DAE+AFLM+
Sbjct: 241 VLLISGDNDPLCPVSVMKDLEKGIGEGSKLVIFGGRGHGFVHNPGSPEEDDDAEKAFLML 300

Query: 301 RNWLHHGLVATN 309
           RNWLHHGLVA N
Sbjct: 301 RNWLHHGLVAIN 303

BLAST of Bhi06G000234 vs. ExPASy TrEMBL
Match: A0A5A7TB03 (Carboxymethylenebutenolidase-like protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold169G00030 PE=4 SV=1)

HSP 1 Score: 493.4 bits (1269), Expect = 6.8e-136
Identity = 252/312 (80.77%), Postives = 268/312 (85.90%), Query Frame = 0

Query: 1   MGLASAGAQLISLPPTGSSSCRRRIGTPFQLPYFRFTSPLPFRKCGNRSDWKWKGKREKK 60
           MG ASAGAQLISLPPTGSSS  RR+  P Q PY +F  PLP  K  N  DWKWKGK    
Sbjct: 1   MGFASAGAQLISLPPTGSSS--RRVEKPIQPPYSQFAYPLPSWKYNNWGDWKWKGKLR-- 60

Query: 61  GGKVCYSVEGVEDDDDDDAACELVNGMELSIGEQPHHSIPAYLFKAVKNNNGTAILLLSD 120
                YSV+G   DDDDD ACELVNGMELSIGEQPHHSIPAYLFKAVKNNNGTAILLLSD
Sbjct: 61  ----YYSVKGRVKDDDDD-ACELVNGMELSIGEQPHHSIPAYLFKAVKNNNGTAILLLSD 120

Query: 121 LFAFQHSSIRDFAYRLACNGFNVLLPDLIHGDSWKK----QSTKRDMQRIAEDIGTVTKW 180
           LFAFQ SSIRDFAYRLACNGFNVLLPDL++ D W+K     S K++MQRIAEDIGTVTKW
Sbjct: 121 LFAFQDSSIRDFAYRLACNGFNVLLPDLVNEDPWEKPPAQHSAKQEMQRIAEDIGTVTKW 180

Query: 181 LVDEFSAAGLSRKLGIMGFGYGGGQVIEVLARDEGACFCIGASLYGTEVDPSLATKVKVP 240
           L DEFSAAGLSRKLGI+GFG+GGG+VI+VLARD+GACF IGASLYGTEVDPSLATKVKVP
Sbjct: 181 LTDEFSAAGLSRKLGIIGFGHGGGRVIDVLARDKGACFLIGASLYGTEVDPSLATKVKVP 240

Query: 241 VLLISGDNDPLCPVSVMKDLEKRMGEGSKLVIFGGRGHDFVHNPRSPEEDEDAEQAFLMI 300
           VLLISGD+DPLCPVSVMKDLEK MGEGSKLVIF GRGH FVHNP SPEED+DAEQAFLM+
Sbjct: 241 VLLISGDDDPLCPVSVMKDLEKGMGEGSKLVIFEGRGHGFVHNPGSPEEDDDAEQAFLML 300

Query: 301 RNWLHHGLVATN 309
           RNWLHHGLVATN
Sbjct: 301 RNWLHHGLVATN 303

BLAST of Bhi06G000234 vs. ExPASy TrEMBL
Match: A0A1S3CB89 (uncharacterized protein LOC103498731 OS=Cucumis melo OX=3656 GN=LOC103498731 PE=4 SV=1)

HSP 1 Score: 493.4 bits (1269), Expect = 6.8e-136
Identity = 252/312 (80.77%), Postives = 268/312 (85.90%), Query Frame = 0

Query: 1   MGLASAGAQLISLPPTGSSSCRRRIGTPFQLPYFRFTSPLPFRKCGNRSDWKWKGKREKK 60
           MG ASAGAQLISLPPTGSSS  RR+  P Q PY +F  PLP  K  N  DWKWKGK    
Sbjct: 1   MGFASAGAQLISLPPTGSSS--RRVEKPIQPPYSQFAYPLPSWKYNNWGDWKWKGKLR-- 60

Query: 61  GGKVCYSVEGVEDDDDDDAACELVNGMELSIGEQPHHSIPAYLFKAVKNNNGTAILLLSD 120
                YSV+G   DDDDD ACELVNGMELSIGEQPHHSIPAYLFKAVKNNNGTAILLLSD
Sbjct: 61  ----YYSVKGRVKDDDDD-ACELVNGMELSIGEQPHHSIPAYLFKAVKNNNGTAILLLSD 120

Query: 121 LFAFQHSSIRDFAYRLACNGFNVLLPDLIHGDSWKK----QSTKRDMQRIAEDIGTVTKW 180
           LFAFQ SSIRDFAYRLACNGFNVLLPDL++ D W+K     S K++MQRIAEDIGTVTKW
Sbjct: 121 LFAFQDSSIRDFAYRLACNGFNVLLPDLVNEDPWEKPPAQHSAKQEMQRIAEDIGTVTKW 180

Query: 181 LVDEFSAAGLSRKLGIMGFGYGGGQVIEVLARDEGACFCIGASLYGTEVDPSLATKVKVP 240
           L DEFSAAGLSRKLGI+GFG+GGG+VI+VLARD+GACF IGASLYGTEVDPSLATKVKVP
Sbjct: 181 LTDEFSAAGLSRKLGIIGFGHGGGRVIDVLARDKGACFLIGASLYGTEVDPSLATKVKVP 240

Query: 241 VLLISGDNDPLCPVSVMKDLEKRMGEGSKLVIFGGRGHDFVHNPRSPEEDEDAEQAFLMI 300
           VLLISGD+DPLCPVSVMKDLEK MGEGSKLVIF GRGH FVHNP SPEED+DAEQAFLM+
Sbjct: 241 VLLISGDDDPLCPVSVMKDLEKGMGEGSKLVIFEGRGHGFVHNPGSPEEDDDAEQAFLML 300

Query: 301 RNWLHHGLVATN 309
           RNWLHHGLVATN
Sbjct: 301 RNWLHHGLVATN 303

BLAST of Bhi06G000234 vs. ExPASy TrEMBL
Match: A0A6J1EC40 (carboxymethylenebutenolidase homolog OS=Cucurbita moschata OX=3662 GN=LOC111432716 PE=4 SV=1)

HSP 1 Score: 454.9 bits (1169), Expect = 2.7e-124
Identity = 233/309 (75.40%), Postives = 259/309 (83.82%), Query Frame = 0

Query: 1   MGLASAGAQLISLPPTGSSSCRRRIGTPFQLPYFRFTSPLPFRKCGNRSDWKWKGKREKK 60
           MG A+AGAQ IS PPTG S   RR+G PFQ P FRF  P+      + S  + KGKRE K
Sbjct: 1   MGSATAGAQWISAPPTGRSG--RRMGIPFQ-PSFRFAFPI------SNSIGQLKGKREIK 60

Query: 61  GGKVCYSVEGVEDDDDDDAACELVNGMELSIGEQPHHSIPAYLFKAVKNNNGTAILLLSD 120
           GGKVCYS EGV+D+ +++ ACELVNGMELSIGE+ HHSI AYLFKAVKNNNGTAILLL+D
Sbjct: 61  GGKVCYSAEGVKDEIEEEEACELVNGMELSIGEEAHHSITAYLFKAVKNNNGTAILLLTD 120

Query: 121 LFAFQHSSIRDFAYRLACNGFNVLLPDLIHGDSWKKQSTKRDMQRIAEDIGTVTKWLVDE 180
           LFAFQ SS+RDFAYRLACNGFNVLLPD+ HGDS ++Q T        EDI   TKWLVDE
Sbjct: 121 LFAFQDSSVRDFAYRLACNGFNVLLPDVFHGDSCEEQKT-------VEDIAAATKWLVDE 180

Query: 181 FSAAGLSRKLGIMGFGYGGGQVIEVLARDEGACFCIGASLYGTEVDPSLATKVKVPVLLI 240
           FSAAG+SRKLGI+GFGYGGG+VI+VLA+D+G+ F IGASLYGTEV+PSLATKV+VPVLLI
Sbjct: 181 FSAAGISRKLGIVGFGYGGGRVIDVLAQDKGSYFSIGASLYGTEVNPSLATKVEVPVLLI 240

Query: 241 SGDNDPLCPVSVMKDLEKRMGEGSKLVIFGGRGHDFVHNPRSPEEDEDAEQAFLMIRNWL 300
           SGDNDPLCPVSVMKDLEK MGEGSKLVIFGGRGH FVH P SPEEDEDAEQAFLM+RNWL
Sbjct: 241 SGDNDPLCPVSVMKDLEKGMGEGSKLVIFGGRGHGFVHKPGSPEEDEDAEQAFLMLRNWL 293

Query: 301 HHGLVATNI 310
           H GLVATNI
Sbjct: 301 HDGLVATNI 293

BLAST of Bhi06G000234 vs. ExPASy TrEMBL
Match: A0A6J1I908 (carboxymethylenebutenolidase homolog OS=Cucurbita maxima OX=3661 GN=LOC111472595 PE=4 SV=1)

HSP 1 Score: 448.4 bits (1152), Expect = 2.5e-122
Identity = 230/309 (74.43%), Postives = 258/309 (83.50%), Query Frame = 0

Query: 1   MGLASAGAQLISLPPTGSSSCRRRIGTPFQLPYFRFTSPLPFRKCGNRSDWKWKGKREKK 60
           MG ASAGAQ IS PPTG S   RR+G PFQ P FR   P+      + +  + KGKREKK
Sbjct: 1   MGSASAGAQWISAPPTGRSG--RRMGIPFQ-PSFRCAFPI------SNNIGQLKGKREKK 60

Query: 61  GGKVCYSVEGVEDDDDDDAACELVNGMELSIGEQPHHSIPAYLFKAVKNNNGTAILLLSD 120
           GGKVCYS EGV+D+ D + ACELV+GMELSIGE+ HHSI A+LFKAVKNNNGTAILLL+D
Sbjct: 61  GGKVCYSAEGVKDEIDAEEACELVSGMELSIGEEAHHSITAHLFKAVKNNNGTAILLLTD 120

Query: 121 LFAFQHSSIRDFAYRLACNGFNVLLPDLIHGDSWKKQSTKRDMQRIAEDIGTVTKWLVDE 180
           LF+FQ SS+RDFAYRLACNGFNVLLPD+ HGDS ++       Q+  EDIG  TKWLVDE
Sbjct: 121 LFSFQDSSVRDFAYRLACNGFNVLLPDVFHGDSCEE-------QKAVEDIGAATKWLVDE 180

Query: 181 FSAAGLSRKLGIMGFGYGGGQVIEVLARDEGACFCIGASLYGTEVDPSLATKVKVPVLLI 240
           F AAG+SRKLGI+GFGYGGG+VI+VLA+D+G+ F IGASLYGTEV+PSLATKV+VPVLLI
Sbjct: 181 FLAAGISRKLGIVGFGYGGGRVIDVLAQDQGSYFSIGASLYGTEVNPSLATKVEVPVLLI 240

Query: 241 SGDNDPLCPVSVMKDLEKRMGEGSKLVIFGGRGHDFVHNPRSPEEDEDAEQAFLMIRNWL 300
           SGDNDPLCPVSVMKDLEK MGEGSKLVIFGGRGH FVH P SPEEDEDAEQAFLM+RNWL
Sbjct: 241 SGDNDPLCPVSVMKDLEKGMGEGSKLVIFGGRGHGFVHKPGSPEEDEDAEQAFLMLRNWL 293

Query: 301 HHGLVATNI 310
           H GLVATNI
Sbjct: 301 HDGLVATNI 293