Bhi05G000618 (gene) Wax gourd (B227) v1

Overview


Name	Bhi05G000618
Type	gene
Organism	Benincasa hispida (Wax gourd (B227) v1)
Description	Plant invertase/pectin methylesterase inhibitor superfamily
Location	chr5: 25135968 .. 25136314 (-)
RNA-Seq Expression	Bhi05G000618
Synteny	Bhi05G000618

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGCTTCTCCAGAGCTTGGGCCAACGAAGGAACCTCGTGATTTTGAAGTTTACCTTGGGTGGCCATGGAGAGATTATGCAAAGGTAATTTTCATGCAGTCATTTCTAGATTCTGTGGTGAATACAGTGGGATGGTCAACATGGTCACCAGAGAGGCCTCTTGATTTACTGTTTTATGGGGAGTATAACAACAGGGTCCAGGAGCTAATTTAGCTGGAAGAGTAAAATGGTCCGGTTTCCATATCATAACTAATCTCACAGAAGCCACTAGCTTTACCATAGGAGTTTTTATTGATGGAAACACATGGTTACCCGAAACCAGTATACCATCTAGACTAGGACTTTAG

mRNA sequence

ATGGCTTCTCCAGAGCTTGGGCCAACGAAGGAACCTCGTGATTTTGAAGTTTACCTTGGGTGGCCATGGAGAGATTATGCAAAGGGTCCAGGAGCTAATTTAGCTGGAAGAGTAAAATGGTCCGGTTTCCATATCATAACTAATCTCACAGAAGCCACTAGCTTTACCATAGGAGTTTTTATTGATGGAAACACATGGTTACCCGAAACCAGTATACCATCTAGACTAGGACTTTAG

Coding sequence (CDS)

ATGGCTTCTCCAGAGCTTGGGCCAACGAAGGAACCTCGTGATTTTGAAGTTTACCTTGGGTGGCCATGGAGAGATTATGCAAAGGGTCCAGGAGCTAATTTAGCTGGAAGAGTAAAATGGTCCGGTTTCCATATCATAACTAATCTCACAGAAGCCACTAGCTTTACCATAGGAGTTTTTATTGATGGAAACACATGGTTACCCGAAACCAGTATACCATCTAGACTAGGACTTTAG

Protein sequence

MASPELGPTKEPRDFEVYLGWPWRDYAKGPGANLAGRVKWSGFHIITNLTEATSFTIGVFIDGNTWLPETSIPSRLGL

Homology

[Close]

BLAST of Bhi05G000618 vs. TAIR 10
Match: AT4G02300.1 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 82.4 bits (202), Expect = 1.7e-16
Identity = 46/114 (40.35%), Postives = 56/114 (49.12%), Query Frame = 0


Query: 1   MASPELGPTKEPRDFEVYLGWPWRDYAK-------------------------------- 60
           +A+P+L P KE  +F+ YLG PWR Y++                                
Sbjct: 420 LAAPDLIPVKE--NFKAYLGRPWRKYSRTVIIKSFIDDLIHPAGWLEGKKDFALETLYYG 479

Query: 61  -----GPGANLAGRVKWSGFHIITNLTEATSFTIGVFIDGNTWLPETSIPSRLG 78
                GPGAN+A RV W GF  I N TEAT FT+G FIDG+TWL  T IP  LG
Sbjct: 480 EYMNEGPGANMAKRVTWPGFRRIENQTEATQFTVGPFIDGSTWLNSTGIPFSLG 531

[Close]

BLAST of Bhi05G000618 vs. TAIR 10
Match: AT4G02320.1 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 66.2 bits (160), Expect = 1.3e-11
Identity = 37/115 (32.17%), Postives = 53/115 (46.09%), Query Frame = 0


Query: 1   MASPELGPTKEPRDFEVYLGWPWRDYAK-------------------------------- 60
           +A+P+L P +   +F+ YLG PW+ Y++                                
Sbjct: 406 LAAPDLIPVQ--ANFKAYLGRPWQLYSRTVIMKSFIDDLVDPAGWLKWKDDFALETLYYG 465

Query: 61  -----GPGANLAGRVKWSGFHIITNLTEATSFTIGVFIDGNTWLPETSIPSRLGL 79
                GPG+N+  RV+W GF  I  + EA+ F++G FIDGN WL  T IP  L L
Sbjct: 466 EYMNEGPGSNMTNRVQWPGFKRIETVEEASQFSVGPFIDGNKWLNSTRIPFTLDL 518

[Close]

BLAST of Bhi05G000618 vs. TAIR 10
Match: AT3G59010.1 (pectin methylesterase 61 )

HSP 1 Score: 64.3 bits (155), Expect = 4.9e-11
Identity = 26/50 (52.00%), Postives = 35/50 (70.00%), Query Frame = 0


Query: 29  GPGANLAGRVKWSGFHIITNLTEATSFTIGVFIDGNTWLPETSIPSRLGL 79
           GPG++++GRV W+G+H    LTEA  FT+  FIDGN+WLP T +    GL
Sbjct: 479 GPGSSVSGRVSWAGYHPALTLTEAQGFTVSGFIDGNSWLPSTGVVFDSGL 528

[Close]

BLAST of Bhi05G000618 vs. TAIR 10
Match: AT2G43050.1 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 63.5 bits (153), Expect = 8.4e-11
Identity = 27/50 (54.00%), Postives = 34/50 (68.00%), Query Frame = 0


Query: 29  GPGANLAGRVKWSGFHIITNLTEATSFTIGVFIDGNTWLPETSIPSRLGL 79
           GPG++++GRVKWSG H    +TEA  FT+  FIDGN WLP T +    GL
Sbjct: 467 GPGSSVSGRVKWSGCHPSLTVTEAEKFTVASFIDGNIWLPSTGVSFDPGL 516

[Close]

BLAST of Bhi05G000618 vs. TAIR 10
Match: AT1G23200.1 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 63.2 bits (152), Expect = 1.1e-10
Identity = 25/50 (50.00%), Postives = 35/50 (70.00%), Query Frame = 0


Query: 29  GPGANLAGRVKWSGFHIITNLTEATSFTIGVFIDGNTWLPETSIPSRLGL 79
           G GA+++GRVKW G+H+I  +TEA  FT+  F+DGN W+  T +P   GL
Sbjct: 505 GAGASVSGRVKWPGYHVIKTVTEAEKFTVENFLDGNYWITATGVPVNDGL 554

[Close]

BLAST of Bhi05G000618 vs. ExPASy Swiss-Prot
Match: O81415 (Probable pectinesterase/pectinesterase inhibitor 39 OS=Arabidopsis thaliana OX=3702 GN=PME39 PE=2 SV=1)

HSP 1 Score: 82.4 bits (202), Expect = 2.5e-15
Identity = 46/114 (40.35%), Postives = 56/114 (49.12%), Query Frame = 0


Query: 1   MASPELGPTKEPRDFEVYLGWPWRDYAK-------------------------------- 60
           +A+P+L P KE  +F+ YLG PWR Y++                                
Sbjct: 420 LAAPDLIPVKE--NFKAYLGRPWRKYSRTVIIKSFIDDLIHPAGWLEGKKDFALETLYYG 479

Query: 61  -----GPGANLAGRVKWSGFHIITNLTEATSFTIGVFIDGNTWLPETSIPSRLG 78
                GPGAN+A RV W GF  I N TEAT FT+G FIDG+TWL  T IP  LG
Sbjct: 480 EYMNEGPGANMAKRVTWPGFRRIENQTEATQFTVGPFIDGSTWLNSTGIPFSLG 531

[Close]

BLAST of Bhi05G000618 vs. ExPASy Swiss-Prot
Match: O04887 (Pectinesterase 2 OS=Citrus sinensis OX=2711 GN=PECS-2.1 PE=2 SV=1)

HSP 1 Score: 66.6 bits (161), Expect = 1.4e-10
Identity = 25/50 (50.00%), Postives = 38/50 (76.00%), Query Frame = 0


Query: 29  GPGANLAGRVKWSGFHIITNLTEATSFTIGVFIDGNTWLPETSIPSRLGL 79
           GPG++ A RVKW G+H++T+ ++ + FT+G FI GN+WLP T++P   GL
Sbjct: 461 GPGSSTANRVKWRGYHVLTSPSQVSQFTVGNFIAGNSWLPATNVPFTSGL 510

[Close]

BLAST of Bhi05G000618 vs. ExPASy Swiss-Prot
Match: O04886 (Pectinesterase 1 OS=Citrus sinensis OX=2711 GN=PECS-1.1 PE=2 SV=1)

HSP 1 Score: 66.2 bits (160), Expect = 1.8e-10
Identity = 38/114 (33.33%), Postives = 51/114 (44.74%), Query Frame = 0


Query: 2   ASPELGPTKEPRDFEVYLGWPWRDYAK--------------------------------- 61
           A+ +L P +    F  YLG PW++Y++                                 
Sbjct: 473 ATSDLKPVQ--GSFPTYLGRPWKEYSRTVIMQSSITDLIHPAGWHEWDGNFALNTLFYGE 532

Query: 62  ----GPGANLAGRVKWSGFHIITNLTEATSFTIGVFIDGNTWLPETSIPSRLGL 79
               G GA  +GRVKW GF +IT+ TEA +FT G FI G++WL  T  P  LGL
Sbjct: 533 HQNSGAGAGTSGRVKWKGFRVITSATEAQAFTPGSFIAGSSWLGSTGFPFSLGL 584

[Close]

BLAST of Bhi05G000618 vs. ExPASy Swiss-Prot
Match: P83948 (Pectinesterase 3 OS=Citrus sinensis OX=2711 PE=1 SV=1)

HSP 1 Score: 66.2 bits (160), Expect = 1.8e-10
Identity = 38/114 (33.33%), Postives = 51/114 (44.74%), Query Frame = 0


Query: 2   ASPELGPTKEPRDFEVYLGWPWRDYAK--------------------------------- 61
           A+ +L P +    F  YLG PW++Y++                                 
Sbjct: 473 ATSDLKPVQ--GSFPTYLGRPWKEYSRTVIMQSSITDVIHPAGWHEWDGNFALNTLFYGE 532

Query: 62  ----GPGANLAGRVKWSGFHIITNLTEATSFTIGVFIDGNTWLPETSIPSRLGL 79
               G GA  +GRVKW GF +IT+ TEA +FT G FI G++WL  T  P  LGL
Sbjct: 533 HQNAGAGAGTSGRVKWKGFRVITSATEAQAFTPGSFIAGSSWLGSTGFPFSLGL 584

[Close]

BLAST of Bhi05G000618 vs. ExPASy Swiss-Prot
Match: O81301 (Probable pectinesterase/pectinesterase inhibitor 40 OS=Arabidopsis thaliana OX=3702 GN=PME40 PE=2 SV=1)

HSP 1 Score: 66.2 bits (160), Expect = 1.8e-10
Identity = 37/115 (32.17%), Postives = 53/115 (46.09%), Query Frame = 0


Query: 1   MASPELGPTKEPRDFEVYLGWPWRDYAK-------------------------------- 60
           +A+P+L P +   +F+ YLG PW+ Y++                                
Sbjct: 406 LAAPDLIPVQ--ANFKAYLGRPWQLYSRTVIMKSFIDDLVDPAGWLKWKDDFALETLYYG 465

Query: 61  -----GPGANLAGRVKWSGFHIITNLTEATSFTIGVFIDGNTWLPETSIPSRLGL 79
                GPG+N+  RV+W GF  I  + EA+ F++G FIDGN WL  T IP  L L
Sbjct: 466 EYMNEGPGSNMTNRVQWPGFKRIETVEEASQFSVGPFIDGNKWLNSTRIPFTLDL 518

[Close]

BLAST of Bhi05G000618 vs. ExPASy TrEMBL
Match: A0A6J1C9Z6 (Pectinesterase OS=Momordica charantia OX=3673 GN=LOC111008805 PE=4 SV=1)

HSP 1 Score: 84.7 bits (208), Expect = 1.8e-13
Identity = 44/113 (38.94%), Postives = 60/113 (53.10%), Query Frame = 0


Query: 3   SPELGPTKEPRDFEVYLGWPWRDYA----------------------------------- 62
           SPELGP K+ ++F+VYLG P RDY+                                   
Sbjct: 204 SPELGPRKD-QNFDVYLGRPSRDYSTVIFMQSFLDTVVNPKGWLEWSLGSPLNLLFYAEF 263

Query: 63  --KGPGANLAGRVKWSGFHIITNLTEATSFTIGVFIDGNTWLPETSIPSRLGL 79
             +GPGA+++ R+KW G+H+I N  EAT +T+  FIDG TWLPET++P R  L
Sbjct: 264 NNRGPGADISKRIKWQGYHLINNPLEATKYTVKDFIDGTTWLPETNVPFRPSL 315

[Close]

BLAST of Bhi05G000618 vs. ExPASy TrEMBL
Match: A0A178V5J8 (Pectinesterase OS=Arabidopsis thaliana OX=3702 GN=AXX17_At4g02900 PE=3 SV=1)

HSP 1 Score: 82.4 bits (202), Expect = 9.1e-13
Identity = 46/114 (40.35%), Postives = 56/114 (49.12%), Query Frame = 0


Query: 1   MASPELGPTKEPRDFEVYLGWPWRDYAK-------------------------------- 60
           +A+P+L P KE  +F+ YLG PWR Y++                                
Sbjct: 420 LAAPDLIPVKE--NFKAYLGRPWRKYSRTVIIKSFIDDLIHPAGWLEWKKDFALETLYYG 479

Query: 61  -----GPGANLAGRVKWSGFHIITNLTEATSFTIGVFIDGNTWLPETSIPSRLG 78
                GPGAN+A RV W GF  I N TEAT FT+G FIDG+TWL  T IP  LG
Sbjct: 480 EYMNEGPGANMAKRVTWPGFRRIENQTEATQFTVGPFIDGSTWLNSTGIPFSLG 531

[Close]

BLAST of Bhi05G000618 vs. ExPASy TrEMBL
Match: A0A654FL61 (Pectinesterase OS=Arabidopsis thaliana OX=3702 GN=AN1_LOCUS17021 PE=3 SV=1)

HSP 1 Score: 82.4 bits (202), Expect = 9.1e-13
Identity = 46/114 (40.35%), Postives = 56/114 (49.12%), Query Frame = 0


Query: 1   MASPELGPTKEPRDFEVYLGWPWRDYAK-------------------------------- 60
           +A+P+L P KE  +F+ YLG PWR Y++                                
Sbjct: 420 LAAPDLIPVKE--NFKAYLGRPWRKYSRTVIIKSFIDDLIHPAGWLEWKKDFALETLYYG 479

Query: 61  -----GPGANLAGRVKWSGFHIITNLTEATSFTIGVFIDGNTWLPETSIPSRLG 78
                GPGAN+A RV W GF  I N TEAT FT+G FIDG+TWL  T IP  LG
Sbjct: 480 EYMNEGPGANMAKRVTWPGFRRIENQTEATQFTVGPFIDGSTWLNSTGIPFSLG 531

[Close]

BLAST of Bhi05G000618 vs. ExPASy TrEMBL
Match: A0A7G2EWT8 (Pectinesterase OS=Arabidopsis thaliana OX=3702 GN=AT9943_LOCUS14629 PE=3 SV=1)

HSP 1 Score: 82.4 bits (202), Expect = 9.1e-13
Identity = 46/114 (40.35%), Postives = 56/114 (49.12%), Query Frame = 0


Query: 1   MASPELGPTKEPRDFEVYLGWPWRDYAK-------------------------------- 60
           +A+P+L P KE  +F+ YLG PWR Y++                                
Sbjct: 420 LAAPDLIPVKE--NFKAYLGRPWRKYSRTVIIKSFIDDLIHPAGWLEWKKDFALETLYYG 479

Query: 61  -----GPGANLAGRVKWSGFHIITNLTEATSFTIGVFIDGNTWLPETSIPSRLG 78
                GPGAN+A RV W GF  I N TEAT FT+G FIDG+TWL  T IP  LG
Sbjct: 480 EYMNEGPGANMAKRVTWPGFRRIENQTEATQFTVGPFIDGSTWLNSTGIPFSLG 531

[Close]

BLAST of Bhi05G000618 vs. ExPASy TrEMBL
Match: A0A1U8B923 (Pectinesterase OS=Nelumbo nucifera OX=4432 GN=LOC104612038 PE=3 SV=1)

HSP 1 Score: 80.9 bits (198), Expect = 2.6e-12
Identity = 41/114 (35.96%), Postives = 58/114 (50.88%), Query Frame = 0


Query: 2   ASPELGPTKEPRDFEVYLGWPWRDYAK--------------------------------- 61
           A+P+L P +  R  E YLG PW+ Y++                                 
Sbjct: 410 AAPDLKPVQ--RSVETYLGRPWQLYSRTVILKTFLDSLINPAGWMEWNGTFALNTLYYGE 469

Query: 62  ----GPGANLAGRVKWSGFHIITNLTEATSFTIGVFIDGNTWLPETSIPSRLGL 79
               GPG++ A RVKW G+H+IT+  EA+ FT+G FIDGN+WLP+T++P   GL
Sbjct: 470 FQNTGPGSSTANRVKWGGYHVITSAAEASQFTVGNFIDGNSWLPDTNVPFTSGL 521

The following BLAST results are available for this feature:

BLAST of Bhi05G000618 vs. TAIR 10


Match Name	E-value	Identity	Description
AT4G02300.1	1.7e-16	40.35	Plant invertase/pectin methylesterase inhibitor superfamily	[more]
AT4G02320.1	1.3e-11	32.17	Plant invertase/pectin methylesterase inhibitor superfamily	[more]
AT3G59010.1	4.9e-11	52.00	pectin methylesterase 61	[more]
AT2G43050.1	8.4e-11	54.00	Plant invertase/pectin methylesterase inhibitor superfamily	[more]
AT1G23200.1	1.1e-10	50.00	Plant invertase/pectin methylesterase inhibitor superfamily	[more]

BLAST of Bhi05G000618 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
O81415	2.5e-15	40.35	Probable pectinesterase/pectinesterase inhibitor 39 OS=Arabidopsis thaliana OX=3...	[more]
O04887	1.4e-10	50.00	Pectinesterase 2 OS=Citrus sinensis OX=2711 GN=PECS-2.1 PE=2 SV=1	[more]
O04886	1.8e-10	33.33	Pectinesterase 1 OS=Citrus sinensis OX=2711 GN=PECS-1.1 PE=2 SV=1	[more]
P83948	1.8e-10	33.33	Pectinesterase 3 OS=Citrus sinensis OX=2711 PE=1 SV=1	[more]
O81301	1.8e-10	32.17	Probable pectinesterase/pectinesterase inhibitor 40 OS=Arabidopsis thaliana OX=3...	[more]

BLAST of Bhi05G000618 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1C9Z6	1.8e-13	38.94	Pectinesterase OS=Momordica charantia OX=3673 GN=LOC111008805 PE=4 SV=1	[more]
A0A178V5J8	9.1e-13	40.35	Pectinesterase OS=Arabidopsis thaliana OX=3702 GN=AXX17_At4g02900 PE=3 SV=1	[more]
A0A654FL61	9.1e-13	40.35	Pectinesterase OS=Arabidopsis thaliana OX=3702 GN=AN1_LOCUS17021 PE=3 SV=1	[more]
A0A7G2EWT8	9.1e-13	40.35	Pectinesterase OS=Arabidopsis thaliana OX=3702 GN=AT9943_LOCUS14629 PE=3 SV=1	[more]
A0A1U8B923	2.6e-12	35.96	Pectinesterase OS=Nelumbo nucifera OX=4432 GN=LOC104612038 PE=3 SV=1	[more]

InterPro

Analysis Name: InterPro Annotations of Wax gourd (B227) v1
Date Performed: 2021-10-22


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR012334	Pectin lyase fold	GENE3D	2.160.20.10		coord: 3..78 e-value: 2.8E-9 score: 38.8
IPR000070	Pectinesterase, catalytic	PFAM	PF01095	Pectinesterase	coord: 28..64 e-value: 1.5E-7 score: 30.5
None	No IPR available	PANTHER	PTHR31707:SF181	PECTINESTERASE	coord: 28..77
None	No IPR available	PANTHER	PTHR31707	PECTINESTERASE	coord: 28..77
IPR011050	Pectin lyase fold/virulence factor	SUPERFAMILY	51126	Pectin lyase-like	coord: 14..74

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Bhi05M000618	Bhi05M000618	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0042545	cell wall modification
molecular_function	GO:0030599	pectinesterase activity