Bhi05G000550 (gene) Wax gourd (B227) v1

Overview
NameBhi05G000550
Typegene
OrganismBenincasa hispida (Wax gourd (B227) v1)
DescriptionPectin lyase-like superfamily protein
Locationchr5: 22200403 .. 22202298 (-)
RNA-Seq ExpressionBhi05G000550
SyntenyBhi05G000550
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCTAACACATCCTCCATCACTTTCTTCTTCTTCTTCTTCTTCTTGTATTTGCTGATACAAACATCCAGTGCAGGCAGCTTCAATGTGATTAGTTTTGGTGCAAAAGGCGACGGGAAAACCGACTCGACAAAATCATTTCTGAAGGCATGGACATCGGCTTGTAGCTCCTCCACTCCATCCACCATCAACGTCCCCAAGGGAAGGTTCTTGCTAACCCCCATCACATTTAGAGGCCCATGTAACAACCATATTACCTTCCGTTTAAATGGCACGCTCGTCGCACCTCTCGATTATCGTGCCCTCGGAAATTCTGGATACTGGATTTTGTTCATCAAAGTTGATGGCGTTTCTTTCTTTGGTGGCAACATTGATGCAAAGGGCGCTGGGTATTGGGCTTGCAAGAAAGCTGGAAAACAGTGTCCAGTTGGAGCTAGGGTATGTCATTAAACAAAGTACTTATGGCTTTATACTCATTTTCTGTCTTCTTTTTTGGTAATCAAATTTAGCTTAATATCTATATACGTCCTAACATCACATCTGTATGCTGATCTCTTATATATATTAATGAAATACAGTCATTGACATTTAATTGGGCAAACAATATTTACGTCAGTGGAATAACCTCGATCAACAGCCAACAAACTCATCTTGTAATCAACAGCTGCAAAAATGTAATGGTTAAAAATGTGAAGGTAATGGCACCCGACCAAAGCCCCAACACTGATGGAATTCATGTCCAGTCGTCTTTTAATGTGACGATCACTGGAAGTACGCTTCAAACCGGAGATGATTGCATATCCATTGGAGATGGAACAAAGAGCCTACTTATGAGTCATGTCAAATGTGGGCCCGGCCATGGTGTAAGGTAATCTATATTGAAAATATGGATTATGTTAAATATTCATGTGATTAATCCAAAAGCTTAAACGGATGGGTTGTGACAAATTTAATTACATTTACCCACTCTAACTATCTTTGAGGTAATAAAAAAGAATATGATTCAATTTAATTTCAATTGATGCATGCAGCATTGGTAGCTTGGGGAAAGAGTTCAATGAAGATGGAGTTGAGAATGTAACATTGACAAATGCAATTTTCATTGGATCCGACAATGGTGTGAGGATAAAGACATGGCCTACGGCTAGCAATGGTTTTGTCAAAAATGTTCTCTTTCAAAATATCATCATGAAGAATGTTAAGAATCCTATCTTAATTGACCAAAACTATTGTCCGAATAACAAAGGTTGTCCTTCTCAGGTAAATTGAATTGTGGTCTTATTCTTTCTCTGATTTCATTATTAAGATTTCATTTGGTAACCATTTTATTTTTTGTTTTTGATTTTTAAAAATTAAGCATATTTCCTCCTCATTTCTTATCGTAATTTGCAAATTCTTAATCAAATTCTAAAACAAAACAAGTTTGTAAAAACTACTTTTTATTTTTTATTTTTTTAAACTTTGGCTTAATTTTTAAACTATTGGTAAAAAGTATATAACAAAGAAAAAATTTAGAAGAGGAAATATTGTATCTCTAGGTTTAATTTAAGGAAAAAAAATCAAAGATTACCAAATGGAGCCTAAATTATTTAATCTTTGCTTATATACATAGTAGTCATTTAATAAACTGAATTAATCTTCAATTTTGTTTGTGTTATATACGTGTTGTTTTATTAGAGTTCTGGAGTGAAGATTAGTGGAGTGACATATAGAAATATACAAGGAACATCAGCAACAATTGAAGCTATGACGTTTGATTGTAGTCCAAGCAATCCATGCAGTGACATACAATTACAAGACATCAAGCTCACATACAAGAACAAGACAACAACATCCTCATGCAAGAATATCGGTGGATCCAGTATTGGAGTACTTGTGCCGGAGAGTTGCTTATGA

mRNA sequence

ATGGCTAACACATCCTCCATCACTTTCTTCTTCTTCTTCTTCTTCTTGTATTTGCTGATACAAACATCCAGTGCAGGCAGCTTCAATGTGATTAGTTTTGGTGCAAAAGGCGACGGGAAAACCGACTCGACAAAATCATTTCTGAAGGCATGGACATCGGCTTGTAGCTCCTCCACTCCATCCACCATCAACGTCCCCAAGGGAAGGTTCTTGCTAACCCCCATCACATTTAGAGGCCCATGTAACAACCATATTACCTTCCGTTTAAATGGCACGCTCGTCGCACCTCTCGATTATCGTGCCCTCGGAAATTCTGGATACTGGATTTTGTTCATCAAAGTTGATGGCGTTTCTTTCTTTGGTGGCAACATTGATGCAAAGGGCGCTGGGTATTGGGCTTGCAAGAAAGCTGGAAAACAGTGTCCAGTTGGAGCTAGGTCATTGACATTTAATTGGGCAAACAATATTTACGTCAGTGGAATAACCTCGATCAACAGCCAACAAACTCATCTTGTAATCAACAGCTGCAAAAATGTAATGGTTAAAAATGTGAAGGTAATGGCACCCGACCAAAGCCCCAACACTGATGGAATTCATGTCCAGTCGTCTTTTAATGTGACGATCACTGGAAGTACGCTTCAAACCGGAGATGATTGCATATCCATTGGAGATGGAACAAAGAGCCTACTTATGAGTCATGTCAAATGTGGGCCCGGCCATGGTGTAAGCATTGGTAGCTTGGGGAAAGAGTTCAATGAAGATGGAGTTGAGAATGTAACATTGACAAATGCAATTTTCATTGGATCCGACAATGGTGTGAGGATAAAGACATGGCCTACGGCTAGCAATGGTTTTGTCAAAAATGTTCTCTTTCAAAATATCATCATGAAGAATGTTAAGAATCCTATCTTAATTGACCAAAACTATTGTCCGAATAACAAAGGTTGTCCTTCTCAGAGTTCTGGAGTGAAGATTAGTGGAGTGACATATAGAAATATACAAGGAACATCAGCAACAATTGAAGCTATGACGTTTGATTGTAGTCCAAGCAATCCATGCAGTGACATACAATTACAAGACATCAAGCTCACATACAAGAACAAGACAACAACATCCTCATGCAAGAATATCGGTGGATCCAGTATTGGAGTACTTGTGCCGGAGAGTTGCTTATGA

Coding sequence (CDS)

ATGGCTAACACATCCTCCATCACTTTCTTCTTCTTCTTCTTCTTCTTGTATTTGCTGATACAAACATCCAGTGCAGGCAGCTTCAATGTGATTAGTTTTGGTGCAAAAGGCGACGGGAAAACCGACTCGACAAAATCATTTCTGAAGGCATGGACATCGGCTTGTAGCTCCTCCACTCCATCCACCATCAACGTCCCCAAGGGAAGGTTCTTGCTAACCCCCATCACATTTAGAGGCCCATGTAACAACCATATTACCTTCCGTTTAAATGGCACGCTCGTCGCACCTCTCGATTATCGTGCCCTCGGAAATTCTGGATACTGGATTTTGTTCATCAAAGTTGATGGCGTTTCTTTCTTTGGTGGCAACATTGATGCAAAGGGCGCTGGGTATTGGGCTTGCAAGAAAGCTGGAAAACAGTGTCCAGTTGGAGCTAGGTCATTGACATTTAATTGGGCAAACAATATTTACGTCAGTGGAATAACCTCGATCAACAGCCAACAAACTCATCTTGTAATCAACAGCTGCAAAAATGTAATGGTTAAAAATGTGAAGGTAATGGCACCCGACCAAAGCCCCAACACTGATGGAATTCATGTCCAGTCGTCTTTTAATGTGACGATCACTGGAAGTACGCTTCAAACCGGAGATGATTGCATATCCATTGGAGATGGAACAAAGAGCCTACTTATGAGTCATGTCAAATGTGGGCCCGGCCATGGTGTAAGCATTGGTAGCTTGGGGAAAGAGTTCAATGAAGATGGAGTTGAGAATGTAACATTGACAAATGCAATTTTCATTGGATCCGACAATGGTGTGAGGATAAAGACATGGCCTACGGCTAGCAATGGTTTTGTCAAAAATGTTCTCTTTCAAAATATCATCATGAAGAATGTTAAGAATCCTATCTTAATTGACCAAAACTATTGTCCGAATAACAAAGGTTGTCCTTCTCAGAGTTCTGGAGTGAAGATTAGTGGAGTGACATATAGAAATATACAAGGAACATCAGCAACAATTGAAGCTATGACGTTTGATTGTAGTCCAAGCAATCCATGCAGTGACATACAATTACAAGACATCAAGCTCACATACAAGAACAAGACAACAACATCCTCATGCAAGAATATCGGTGGATCCAGTATTGGAGTACTTGTGCCGGAGAGTTGCTTATGA

Protein sequence

MANTSSITFFFFFFFLYLLIQTSSAGSFNVISFGAKGDGKTDSTKSFLKAWTSACSSSTPSTINVPKGRFLLTPITFRGPCNNHITFRLNGTLVAPLDYRALGNSGYWILFIKVDGVSFFGGNIDAKGAGYWACKKAGKQCPVGARSLTFNWANNIYVSGITSINSQQTHLVINSCKNVMVKNVKVMAPDQSPNTDGIHVQSSFNVTITGSTLQTGDDCISIGDGTKSLLMSHVKCGPGHGVSIGSLGKEFNEDGVENVTLTNAIFIGSDNGVRIKTWPTASNGFVKNVLFQNIIMKNVKNPILIDQNYCPNNKGCPSQSSGVKISGVTYRNIQGTSATIEAMTFDCSPSNPCSDIQLQDIKLTYKNKTTTSSCKNIGGSSIGVLVPESCL
Homology
BLAST of Bhi05G000550 vs. TAIR 10
Match: AT2G43890.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 541.6 bits (1394), Expect = 5.3e-154
Identity = 253/392 (64.54%), Postives = 316/392 (80.61%), Query Frame = 0

Query: 1   MANTSSITFFFFFFFLYLLIQTSSAGS-FNVISFGAKGDGKTDSTKSFLKAWTSACSSST 60
           M N   +     FF  +LL+++S+A S +NV+SFGAK DG+TDSTK+FL AW +AC S+ 
Sbjct: 1   MDNNKLVAVLLMFFSSFLLMKSSTAASNYNVVSFGAKPDGRTDSTKAFLGAWQAACRSAA 60

Query: 61  PSTINVPKGRFLLTPITFRGPCNNHITFRLNGTLVAPLDYRALGNSGYWILFIKVDGVSF 120
             T+ VP+G FLL P+ FRGPC + ITF++ GT+VAP DYR LGNSGYWILF+KV+ +S 
Sbjct: 61  AVTVTVPRGSFLLKPVEFRGPCRSRITFQIYGTIVAPSDYRGLGNSGYWILFVKVNRISI 120

Query: 121 FGGNIDAKGAGYWACKKAGKQCPVGARSLTFNWANNIYVSGITSINSQQTHLVINSCKNV 180
            GG +DA+GA +WAC+K+GK CPVGARS+TFNWAN++ VSG+TSINSQ THLVINSC NV
Sbjct: 121 IGGTLDARGASFWACRKSGKSCPVGARSMTFNWANDVVVSGLTSINSQTTHLVINSCNNV 180

Query: 181 MVKNVKVMAPDQSPNTDGIHVQSSFNVTITGSTLQTGDDCISIGDGTKSLLMSHVKCGPG 240
           +V+ VK++APDQSPNTDG+HVQ S  VT+T  T  TGDDCISIG GT++L MS + CGPG
Sbjct: 181 IVRKVKLVAPDQSPNTDGLHVQGSAGVTVTDGTFHTGDDCISIGPGTRNLYMSKLNCGPG 240

Query: 241 HGVSIGSLGKEFNEDGVENVTLTNAIFIGSDNGVRIKTWPTASNGFVKNVLFQNIIMKNV 300
           HG+SIGSLG++ NE GVEN+TL N++F GSDNGVRIKTW   S GFV+NVLFQN+IMKNV
Sbjct: 241 HGISIGSLGRDANEAGVENITLINSVFSGSDNGVRIKTWARQSTGFVRNVLFQNLIMKNV 300

Query: 301 KNPILIDQNYCPNNKGCPSQSSGVKISGVTYRNIQGTSATIEAMTFDCSPSNPCSDIQLQ 360
           +NPI++DQNYCP+N+GCP Q SGVKIS V YRNIQGTS T +A+TFDCS SNPC  I+L 
Sbjct: 301 QNPIIVDQNYCPSNQGCPKQGSGVKISQVVYRNIQGTSRTQQALTFDCSRSNPCQAIRLH 360

Query: 361 DIKLTYKNKTTTSSCKNIGGSSIGVLVPESCL 392
           DIKLT+  ++ TS+CKNI G   GV++P+ CL
Sbjct: 361 DIKLTFNGRSATSTCKNIKGVKAGVVMPQGCL 392

BLAST of Bhi05G000550 vs. TAIR 10
Match: AT1G05660.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 474.2 bits (1219), Expect = 1.0e-133
Identity = 232/391 (59.34%), Postives = 295/391 (75.45%), Query Frame = 0

Query: 4   TSSITFFFFFFFLYLLIQTSSAGS--FNVISFGAKGDGKTDSTKSFLKAWTSACSSSTPS 63
           T S+  F   F L   I  S + S  FNV+SFGAK DG TDST +FLKAW  AC S++ +
Sbjct: 2   TKSVIRFSLLFTLLTFIDVSISASNVFNVVSFGAKPDGVTDSTGAFLKAWQGACVSASSA 61

Query: 64  TINVPKGRFLLTPITFRGPCNNHITFRLNGTLVAPLDYRALGNSGYWILFIKVDGVSFFG 123
           T+ VPKG FLL  ITF GPC + ITF++ GT++AP DYR  GNSG+WILF KV+  S  G
Sbjct: 62  TVVVPKGTFLLKVITFGGPCKSKITFQVAGTVIAPEDYRTFGNSGFWILFNKVNRFSLVG 121

Query: 124 GNIDAKGAGYWACKKAGKQCPVGARSLTFNWANNIYVSGITSINSQQTHLVINSCKNVMV 183
           G  DA+  G+W+C+K+G+ CP G RS++FN A ++ +SG+ S+NSQ TH+ +N C NV+V
Sbjct: 122 GTFDARANGFWSCRKSGQNCPPGVRSISFNSAKDVIISGVKSMNSQVTHMTLNGCTNVVV 181

Query: 184 KNVKVMAPDQSPNTDGIHVQSSFNVTITGSTLQTGDDCISIGDGTKSLLMSHVKCGPGHG 243
           +NVK++AP  SPNTDG HVQ S  VT TGST+QTGDDC++IG GT++LL++ + CGPGHG
Sbjct: 182 RNVKLVAPGNSPNTDGFHVQHSTGVTFTGSTVQTGDDCVAIGPGTRNLLITKLACGPGHG 241

Query: 244 VSIGSLGKEFNEDGVENVTLTNAIFIGSDNGVRIKTWPTASNGFVKNVLFQNIIMKNVKN 303
           VSIGSL KE  EDGVENVT+++++F GS NGVRIK+W   SNGFV+ V FQ+++MKNV+N
Sbjct: 242 VSIGSLAKELKEDGVENVTVSSSVFTGSQNGVRIKSWARPSNGFVRTVFFQDLVMKNVEN 301

Query: 304 PILIDQNYCPNNKGCPSQSSGVKISGVTYRNIQGTSATIEAMTFDCSPSNPCSDIQLQDI 363
           PI+IDQNYCP ++GCP++ SGVKIS VTY+NIQGTSAT EAM   CS S+PC+ I LQDI
Sbjct: 302 PIIIDQNYCPTHEGCPNEYSGVKISQVTYKNIQGTSATQEAMKLVCSKSSPCTGITLQDI 361

Query: 364 KLTY-KNKTTTSSCKNIGGSSIGVLVPESCL 392
           KLTY K    TS C N  G S+GV+ P SCL
Sbjct: 362 KLTYNKGTPATSFCFNAVGKSLGVIQPTSCL 392

BLAST of Bhi05G000550 vs. TAIR 10
Match: AT1G05650.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 473.4 bits (1217), Expect = 1.8e-133
Identity = 231/389 (59.38%), Postives = 296/389 (76.09%), Query Frame = 0

Query: 5   SSITFFFFFFFLYLLIQTSSAG-SFNVISFGAKGDGKTDSTKSFLKAWTSACSSSTPSTI 64
           S+ITF   F  L  +  +SSA   FNV+SFGAK DG TDST +FLKAW  AC S+  +T+
Sbjct: 4   SAITFPLIFTLLTFIDVSSSASIVFNVVSFGAKPDGVTDSTAAFLKAWQGACGSAASATV 63

Query: 65  NVPKGRFLLTPITFRGPCNNHITFRLNGTLVAPLDYRALGNSGYWILFIKVDGVSFFGGN 124
            VP G FLL  ITF GPC + ITF++ GT+VAP DYR  GNSG WILF KV+  S  GG 
Sbjct: 64  VVPTGTFLLKVITFGGPCKSKITFQVTGTVVAPEDYRTFGNSGSWILFNKVNRFSLVGGT 123

Query: 125 IDAKGAGYWACKKAGKQCPVGARSLTFNWANNIYVSGITSINSQQTHLVINSCKNVMVKN 184
            DA+G+G+W+C+K+G+ CP G RS++FN A ++ +SG+ S+NSQ +H+ +N C NV V+N
Sbjct: 124 FDARGSGFWSCRKSGQNCPPGVRSISFNSAKDVIISGVKSMNSQVSHMTLNGCTNVAVRN 183

Query: 185 VKVMAPDQSPNTDGIHVQSSFNVTITGSTLQTGDDCISIGDGTKSLLMSHVKCGPGHGVS 244
           ++++AP  SPNTDG  VQ S  VT+TGST+QTGDDC++IG GT++ L+S + CGPGHGVS
Sbjct: 184 IRLVAPGDSPNTDGFTVQFSTGVTLTGSTVQTGDDCVAIGQGTRNFLISKLACGPGHGVS 243

Query: 245 IGSLGKEFNEDGVENVTLTNAIFIGSDNGVRIKTWPTASNGFVKNVLFQNIIMKNVKNPI 304
           IGSL K+ NEDGVENVT+++++F GS NGVRIK+W   S GFV+NV FQN+IM+NV+NPI
Sbjct: 244 IGSLAKQLNEDGVENVTVSSSVFTGSQNGVRIKSWARPSTGFVRNVFFQNLIMRNVQNPI 303

Query: 305 LIDQNYCPNNKGCPSQSSGVKISGVTYRNIQGTSATIEAMTFDCSPSNPCSDIQLQDIKL 364
           +IDQNYCP+N+GCP++ SGVKI+ VTY+NIQGTSAT EAM   CS SNPC+ I LQDIKL
Sbjct: 304 IIDQNYCPSNQGCPTEHSGVKITQVTYKNIQGTSATQEAMKLVCSKSNPCTGITLQDIKL 363

Query: 365 TY-KNKTTTSSCKNIGGSSIGVLVPESCL 392
           TY K    TS C N  G ++GV+ P SCL
Sbjct: 364 TYNKGTPATSLCFNAVGKNLGVIQPTSCL 392

BLAST of Bhi05G000550 vs. TAIR 10
Match: AT2G43880.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 463.0 bits (1190), Expect = 2.4e-130
Identity = 219/394 (55.58%), Postives = 294/394 (74.62%), Query Frame = 0

Query: 1   MANTSSI--TFFFFFFFLYLLIQTSSA-GSFNVISFGAKGDGKTDSTKSFLKAWTSACSS 60
           MAN  S      FF   ++ LI++S A  SFNV  +GA+GDG+ D+TKSFL AW+ AC S
Sbjct: 1   MANNISFPCAIIFFSINIFFLIKSSHAMPSFNVQRYGARGDGRADATKSFLTAWSLACGS 60

Query: 61  STPSTINVPKGRFLLTPITFRGPCNNHITFRLNGTLVAPLDYRALGNSGYWILFIKVDGV 120
              + + VP+G +L+  + F GPC N ITF+ +GTLVAP +Y  +GNSGYWILF KV+ +
Sbjct: 61  RARAMVYVPRGTYLVKNLVFWGPCKNIITFKNDGTLVAPANYWDIGNSGYWILFAKVNRI 120

Query: 121 SFFGGNIDAKGAGYWACKKAGKQCPVGARSLTFNWANNIYVSGITSINSQQTHLVINSCK 180
           S +GG IDA+GAGYW+C+K G  CP GARS++F+W NN+ +SG++S NSQ  H+ ++   
Sbjct: 121 SVYGGTIDARGAGYWSCRKKGSHCPQGARSISFSWCNNVLLSGLSSFNSQNMHVTVHHSS 180

Query: 181 NVMVKNVKVMAPDQSPNTDGIHVQSSFNVTITGSTLQTGDDCISIGDGTKSLLMSHVKCG 240
           NV ++NV++ AP  SPNTDGIHVQSS  VTI+G T+ TGDDCI++  G++++ +  V CG
Sbjct: 181 NVRIENVRIRAPSGSPNTDGIHVQSSSGVTISGGTIATGDDCIALSQGSRNIWIERVNCG 240

Query: 241 PGHGVSIGSLGKEFNEDGVENVTLTNAIFIGSDNGVRIKTWPTASNGFVKNVLFQNIIMK 300
           PGHG+SIGSLG   NE+GV+NVT+T+++F  + NGVRIKTW   S GFV NV+F+N+IM 
Sbjct: 241 PGHGISIGSLGDYANEEGVQNVTVTSSVFTKTQNGVRIKTWARPSRGFVNNVVFRNLIMN 300

Query: 301 NVKNPILIDQNYCPNNKGCPSQSSGVKISGVTYRNIQGTSATIEAMTFDCSPSNPCSDIQ 360
           NV+NP++IDQNYCPN KGCP QSSGVKISGVT+ NI+GTS T  AM  DCS SN C+ ++
Sbjct: 301 NVENPVIIDQNYCPNGKGCPRQSSGVKISGVTFANIKGTSTTPIAMKLDCSGSNHCTGLR 360

Query: 361 LQDIKLTYKNKTTTSSCKNIGGSSIGVLVPESCL 392
           LQDIKLTY  +++ S C+N  G + GV+VP +C+
Sbjct: 361 LQDIKLTYMRRSSASYCRNAHGRASGVMVPRNCM 394

BLAST of Bhi05G000550 vs. TAIR 10
Match: AT3G59850.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 439.5 bits (1129), Expect = 2.8e-123
Identity = 213/379 (56.20%), Postives = 271/379 (71.50%), Query Frame = 0

Query: 16  LYLL-IQTSSAGSFNVISFGAKGDGKTDSTKSFLKAWTSACSSSTPSTINVPKGRFLLTP 75
           L+LL + +SSA ++N++S+GAK DGKTDSTK+F   W  AC+S  P TI VPKGRFLL  
Sbjct: 10  LFLLSVSSSSAQTYNILSYGAKPDGKTDSTKAFTVLWAKACASVKPVTILVPKGRFLLRS 69

Query: 76  ITFRG-PC-NNHITFRLNGTLVAPLDYRALGNSGYWILFIKVDGVSFFGGNIDAKGAGYW 135
           I F G  C    +TFR+ GTLVAP DYR +G   YWILF  +DG+S +GG +DA+GA  W
Sbjct: 70  IIFDGSKCKRKSVTFRIQGTLVAPSDYRVIGKENYWILFQHLDGISVYGGVLDAQGASLW 129

Query: 136 ACKKAGKQCPVGARSLTFNWANNIYVSGITSINSQQTHLVINSCKNVMVKNVKVMAPDQS 195
           +CKK+GK CP GA S+ F  + N+ +SG+TS+NSQ  H+ IN C NV +  VKV A   S
Sbjct: 130 SCKKSGKNCPSGATSIGFQSSRNVVISGLTSLNSQMFHVAINGCSNVKLDGVKVSADGNS 189

Query: 196 PNTDGIHVQSSFNVTITGSTLQTGDDCISIGDGTKSLLMSHVKCGPGHGVSIGSLGKEFN 255
           PNTDGIHVQSS  V+I  S + TGDDC+SIG GT  L + +V CGPGHG+SIGSLGKE  
Sbjct: 190 PNTDGIHVQSSSTVSILNSKISTGDDCVSIGPGTNGLWIENVACGPGHGISIGSLGKESV 249

Query: 256 EDGVENVTLTNAIFIGSDNGVRIKTWPTASNGFVKNVLFQNIIMKNVKNPILIDQNYCPN 315
           E GV+N+T+  A F G++NGVRIK+W   SNGF KN+ FQ+ +M NV+NPI+IDQNYCP 
Sbjct: 250 EVGVQNITVKTATFTGTENGVRIKSWARPSNGFAKNIRFQHCVMNNVQNPIVIDQNYCPG 309

Query: 316 NKGCPSQSSGVKISGVTYRNIQGTSATIEAMTFDCSPSNPCSDIQLQDIKLTYKNKTTTS 375
           N+ CP+Q SG+KIS V + +I GTSAT   +  DCS   PC+ I++QD+KLTY+NK  T+
Sbjct: 310 NENCPNQVSGIKISDVMFFDIHGTSATEVGVKLDCSSKKPCTGIRIQDVKLTYRNKPATT 369

Query: 376 SCKNIGGSSIGVLVPESCL 392
            C + GGS  G   P SCL
Sbjct: 370 DCSHAGGSEAGFQRPNSCL 388

BLAST of Bhi05G000550 vs. ExPASy Swiss-Prot
Match: P48979 (Polygalacturonase OS=Prunus persica OX=3760 PE=2 SV=1)

HSP 1 Score: 427.2 bits (1097), Expect = 2.0e-118
Identity = 215/393 (54.71%), Postives = 271/393 (68.96%), Query Frame = 0

Query: 1   MANTSSITFFFFFFFLYLLIQTSSAGSFNVISFGAKGDGKTDSTKSFLKAWTSACSSSTP 60
           MAN  S+      F   +    +S  ++NV S GAK DGKTDSTK+FL AW  AC+S  P
Sbjct: 1   MANRRSLFSLSLIFVFMINSAIASPLTYNVASLGAKADGKTDSTKAFLSAWAKACASMNP 60

Query: 61  STINVPKGRFLLTPITFRGPC-NNHITFRLNGTLVAPLDYRALGNSGYWILFIKVDGVSF 120
             I VP G F L  + F GPC NN ITFR+ GTLVAP DYR +GN+  WI F  V+GV+ 
Sbjct: 61  GVIYVPAGTFFLRDVVFSGPCKNNAITFRIAGTLVAPSDYRVIGNAANWIFFHHVNGVTI 120

Query: 121 FGGNIDAKGAGYWACKKA-GKQCPVGARSLTFNWANNIYVSGITSINSQQTHLVINSCKN 180
            GG +D +G   WACK   G+ CP GA +L F+ +NNI VSG+ S+NSQ  H+VIN  +N
Sbjct: 121 SGGILDGQGTALWACKACHGESCPSGATTLGFSDSNNIVVSGLASLNSQMFHIVINDFQN 180

Query: 181 VMVKNVKVMAPDQSPNTDGIHVQSSFNVTITGSTLQTGDDCISIGDGTKSLLMSHVKCGP 240
           V ++ V+V     SPNTDGIHVQ S  VTI  S + TGDDC+SIG GT +L +  V CGP
Sbjct: 181 VQMQGVRVSRSGNSPNTDGIHVQMSSGVTILNSKIATGDDCVSIGPGTSNLWIEGVACGP 240

Query: 241 GHGVSIGSLGKEFNEDGVENVTLTNAIFIGSDNGVRIKTWPTASNGFVKNVLFQNIIMKN 300
           GHG+SIGSLGKE  E GV+NVT+    F G+ NG+RIK+W   S GF +N+LFQ+  M N
Sbjct: 241 GHGISIGSLGKEQEEAGVQNVTVKTVTFSGTQNGLRIKSWGRPSTGFARNILFQHATMVN 300

Query: 301 VKNPILIDQNYCPNNKGCPSQSSGVKISGVTYRNIQGTSATIEAMTFDCSPSNPCSDIQL 360
           V+NPI+IDQ+YCP+NKGCP Q SGV+IS VTY +I GTSAT  A+ FDCSP +PC +I+L
Sbjct: 301 VENPIVIDQHYCPDNKGCPGQVSGVQISDVTYEDIHGTSATEVAVKFDCSPKHPCREIKL 360

Query: 361 QDIKLTYKNKTTTSSCKNIGGSSIGVLVPESCL 392
           +D+KLTYKN+   SSC +  G++ GV+ P SCL
Sbjct: 361 EDVKLTYKNQAAESSCSHADGTTEGVVQPTSCL 393

BLAST of Bhi05G000550 vs. ExPASy Swiss-Prot
Match: O22818 (Probable polygalacturonase At2g43860 OS=Arabidopsis thaliana OX=3702 GN=At2g43860 PE=2 SV=1)

HSP 1 Score: 374.4 bits (960), Expect = 1.6e-102
Identity = 189/368 (51.36%), Postives = 255/368 (69.29%), Query Frame = 0

Query: 27  SFNVISFGAKGDGKTDSTKSFLKAWTSACSSSTPSTINVPKGRFLLTPITFRG-PCNN-H 86
           + NV+S+GAK DG  DSTK+FL AW  AC+S+ P+TI VPKGRFL+  + F G  C    
Sbjct: 32  TLNVLSYGAKPDGSKDSTKAFLAAWDVACASANPTTIIVPKGRFLVGNLVFHGNECKQAP 91

Query: 87  ITFRLNGTLVAPLDYRALGNSGYWILFIKVDGVSFFGGNIDAKGAGYWACK-KAGKQCPV 146
           I+ R+ G++VAP D+R + +S +WI F  V  VS +GG +DA+G   W CK   G  CP 
Sbjct: 92  ISIRIAGSIVAPEDFRIIASSKHWIWFEDVTDVSIYGGILDAQGTSLWKCKNNGGHNCPT 151

Query: 147 GARSLTFNWANNIYVSGITSINSQQTHLVINSCKNVMVKNVKVMAPDQSPNTDGIHVQSS 206
           GA+SL F+ +NNI +SG+TSINSQ+ H+VI++  NV +  VKV A + SPNTDGIHV+SS
Sbjct: 152 GAKSLVFSGSNNIKISGLTSINSQKFHIVIDNSNNVNIDGVKVSADENSPNTDGIHVESS 211

Query: 207 FNVTITGSTLQTGDDCISIGDGTKSLLMSHVKCGPGHGVSIGSLGKEFNEDGVENVTLTN 266
            +V IT S + TGDDCISIG G+ ++ +  ++CGPGHG+SIGSLG+   E GV+NVT++N
Sbjct: 212 HSVHITNSRIGTGDDCISIGPGSTNVFIQTIRCGPGHGISIGSLGRAEEEQGVDNVTVSN 271

Query: 267 AIFIGSDNGVRIKTWPTASNGFVKNVLFQNIIMKNVKNPILIDQNYCPNNKGCPSQSSGV 326
             F+G++NGVRIKTW   SN F +N++FQ+I MK VKNPI+IDQ+YC  +K CP Q SGV
Sbjct: 272 VDFMGTNNGVRIKTWGKDSNSFARNIVFQHINMKMVKNPIIIDQHYC-LHKPCPKQESGV 331

Query: 327 KISGVTYRNIQGTSATIEAMTFDCSPSNPCSDIQLQDIKLTYKNKTTTSSCKNIGGSSIG 386
           K+S V Y +I GTS T  A+  DCS   PC+ I + D+ L   ++   +SC N  GS+  
Sbjct: 332 KVSNVRYEDIHGTSNTEVAVLLDCSKEKPCTGIVMDDVNLVSVHRPAQASCDNANGSAND 391

Query: 387 VLVPESCL 392
           V+    CL
Sbjct: 392 VVPFTPCL 398

BLAST of Bhi05G000550 vs. ExPASy Swiss-Prot
Match: Q39766 (Polygalacturonase OS=Gossypium barbadense OX=3634 GN=G9 PE=2 SV=1)

HSP 1 Score: 310.8 bits (795), Expect = 2.1e-83
Identity = 166/388 (42.78%), Postives = 240/388 (61.86%), Query Frame = 0

Query: 11  FFFFFLYLLIQTSSAGSFNVIS-FGAKGDGKTDSTKSFLKAWTSACSSSTPSTINVPKGR 70
           F    L++        +F+V++ FGAK DGKTD +K FL AW  AC+S TPST+ +PKG 
Sbjct: 12  FVLLLLFISASKVQPDAFDVVAKFGAKADGKTDLSKPFLDAWKEACASVTPSTVVIPKGT 71

Query: 71  FLLTPITFRGPCNNHITFRLNGTLVAPLDYRALGNSGYWILFIKVDGVSFFGGNI-DAKG 130
           +LL+ +   GPC   I   + GT+ AP D  A  +   W+ F  V+    FGG I D +G
Sbjct: 72  YLLSKVNLEGPCKAPIEINVQGTIQAPADPSAFKDPN-WVRFYSVENFKMFGGGIFDGQG 131

Query: 131 AGYW---AC--KKAGKQCPVGARSLTFNWANNIYVSGITSINSQQTHLVINSCKNVMVKN 190
           +  +    C  ++   + PV  R   F++  N  +  ITS +S+  H+ + +CKN+ ++ 
Sbjct: 132 SIAYEKNTCENREFRSKLPVNIR---FDFVTNALIQDITSKDSKLFHINVFACKNITLER 191

Query: 191 VKVMAPDQSPNTDGIHVQSSFNVTITGSTLQTGDDCISIGDGTKSLLMSHVKCGPGHGVS 250
           +K+ APD+SPNTDGIH+  S  V I  S ++TGDDCISIGDGTK++++  + CGPGHG+S
Sbjct: 192 LKIEAPDESPNTDGIHMGKSEGVNIIASDIKTGDDCISIGDGTKNMVIKEITCGPGHGIS 251

Query: 251 IGSLGKEFNEDGVENVTLTNAIFIGSDNGVRIKTWPTASNGFVKNVLFQNIIMKNVKNPI 310
           IGSLGK  NE+ VE + ++N     + NG RIKTWP    G V  + F++I M NV +PI
Sbjct: 252 IGSLGKFQNEEPVEGIKISNCTITNTSNGARIKTWPGEHGGAVSEIHFEDITMNNVSSPI 311

Query: 311 LIDQNYCPNNKGCPSQSSGVKISGVTYRNIQGTSATIEAMTFDCSPSNPCSDIQLQDIKL 370
           LIDQ YCP NK   ++ S VK+S ++++NI+GTSA  EA+ F CS S+PC +++L DI +
Sbjct: 312 LIDQQYCPWNKCKKNEESKVKLSNISFKNIRGTSALPEAIKFICSGSSPCQNVELADIDI 371

Query: 371 TYKN-KTTTSSCKNIGGSSIGVLVPESC 391
            +   +  TS C N+   +IG L P  C
Sbjct: 372 QHNGAEPATSQCLNVKPITIGKLNPIPC 395

BLAST of Bhi05G000550 vs. ExPASy Swiss-Prot
Match: Q39786 (Polygalacturonase OS=Gossypium hirsutum OX=3635 GN=G9 PE=2 SV=1)

HSP 1 Score: 308.9 bits (790), Expect = 8.1e-83
Identity = 165/388 (42.53%), Postives = 240/388 (61.86%), Query Frame = 0

Query: 11  FFFFFLYLLIQTSSAGSFNVIS-FGAKGDGKTDSTKSFLKAWTSACSSSTPSTINVPKGR 70
           F    L++      + +F+V++ FGAK DGKTD +K FL AW  AC+S TPST+ +PKG 
Sbjct: 12  FVLLLLFISASKVQSDAFDVVAKFGAKADGKTDLSKPFLDAWKEACASVTPSTVVIPKGT 71

Query: 71  FLLTPITFRGPCNNHITFRLNGTLVAPLDYRALGNSGYWILFIKVDGVSFFGGNI-DAKG 130
           +LL+ +   GPC   I   + GT+ AP D  A  +   W+ F  V+    FGG I D +G
Sbjct: 72  YLLSKVNLEGPCKAPIEINVQGTIQAPADPSAFKDPN-WVRFYSVENFKMFGGGIFDGQG 131

Query: 131 AGYW---AC--KKAGKQCPVGARSLTFNWANNIYVSGITSINSQQTHLVINSCKNVMVKN 190
           +  +    C  ++   + PV  R   F++  N  +  ITS +S+  H+ + +CKN+ ++ 
Sbjct: 132 SIAYEKNTCENREFRSKLPVNIR---FDFLTNALIQDITSKDSKLFHINVFACKNITLER 191

Query: 191 VKVMAPDQSPNTDGIHVQSSFNVTITGSTLQTGDDCISIGDGTKSLLMSHVKCGPGHGVS 250
           +K+ APD+SPNTDGIH+  S  V I  S ++TGDDCISIGDGTK++++  + CGPGHG+S
Sbjct: 192 LKIEAPDESPNTDGIHMGKSEGVNIIASDIKTGDDCISIGDGTKNMVIKEITCGPGHGIS 251

Query: 251 IGSLGKEFNEDGVENVTLTNAIFIGSDNGVRIKTWPTASNGFVKNVLFQNIIMKNVKNPI 310
           IGSLGK  NE+ VE + ++N     + NG RIKTWP    G V  + F++I M NV +PI
Sbjct: 252 IGSLGKFQNEEPVEGIKISNCTITNTSNGARIKTWPGEHGGAVSEIHFEDITMNNVSSPI 311

Query: 311 LIDQNYCPNNKGCPSQSSGVKISGVTYRNIQGTSATIEAMTFDCSPSNPCSDIQLQDIKL 370
           LIDQ YCP NK   ++ S VK+S ++++NI+GTSA  EA+ F CS S+PC +++L DI +
Sbjct: 312 LIDQQYCPWNKCKKNEESKVKLSNISFKNIRGTSALPEAIKFICSGSSPCQNVELADIDI 371

Query: 371 TYKN-KTTTSSCKNIGGSSIGVLVPESC 391
            +   +  TS C N+   + G L P  C
Sbjct: 372 KHNGAEPATSQCLNVKPITSGKLNPIPC 395

BLAST of Bhi05G000550 vs. ExPASy Swiss-Prot
Match: O23147 (Polygalacturonase ADPG1 OS=Arabidopsis thaliana OX=3702 GN=ADPG1 PE=2 SV=1)

HSP 1 Score: 291.2 bits (744), Expect = 1.7e-77
Identity = 162/374 (43.32%), Postives = 228/374 (60.96%), Query Frame = 0

Query: 22  TSSAGSFNVISFGAKGDGKTDSTKSFLKAWTSACSSSTPSTINVPKGR-FLLTPITFRGP 81
           T  A + +V +FGAKGDGKTD T++F KAW  ACS++  +T  VPKG+ +LL    FRGP
Sbjct: 62  TLEASTVSVSNFGAKGDGKTDDTQAFKKAWKKACSTNGVTTFLVPKGKTYLLKSTRFRGP 121

Query: 82  CNNHITFRLNGTLVAPLDYRALGNSGYWILFIKVDGVSFFGGN---IDAKGAGYW--ACK 141
           C +   F++ GTL A        +  +W++   V+ +S  GG+   I+  G  +W  +CK
Sbjct: 122 CKSLRNFQILGTLSASTKRSDYKDKNHWLILEDVNNLSIDGGSTGIINGNGKTWWQNSCK 181

Query: 142 -KAGKQCPVGARSLTFNWANNIYVSGITSINSQQTHLVINSCKNVMVKNVKVMAPDQSPN 201
               K C     +LT     N+ V  +   N+QQ  + I  C  V V NV++ AP  SPN
Sbjct: 182 IDKSKPCTKAPTALTLYNLKNLNVKNLRVKNAQQIQISIEKCNKVEVSNVEITAPGDSPN 241

Query: 202 TDGIHVQSSFNVTITGSTLQTGDDCISIGDGTKSLLMSHVKCGPGHGVSIGSLGKEFNED 261
           TDGIH+ ++ N+ ++ S + TGDDCISI DGT++L +  + CGPGHG+SIGSLG + ++ 
Sbjct: 242 TDGIHITNTQNIRVSNSDIGTGDDCISIEDGTQNLQIFDLTCGPGHGISIGSLGDDNSKA 301

Query: 262 GVENVTLTNAIFIGSDNGVRIKTWPTASNGFVKNVLFQNIIMKNVKNPILIDQNYCPNNK 321
            V  + +  A F  SDNGVRIKT+   S G  KN+ FQNI M+NVKNPI+IDQ+YC  +K
Sbjct: 302 YVSGINVDGAKFSESDNGVRIKTYQGGS-GTAKNIKFQNIRMENVKNPIIIDQDYCDKDK 361

Query: 322 GCPSQSSGVKISGVTYRNIQGTSATIEAMTFDCSPSNPCSDIQLQDIKLTYKNKTTTSSC 381
            C  Q S V++  V Y+NI GTSAT  A+T +CS   PC  I L+++K+    K  T+SC
Sbjct: 362 -CEDQESAVQVKNVVYKNISGTSATDVAITLNCSEKYPCQGIVLENVKI----KGGTASC 421

Query: 382 KNIGGSSIGVLVPE 389
           KN    + G + P+
Sbjct: 422 KNANVKNQGTVSPK 429

BLAST of Bhi05G000550 vs. ExPASy TrEMBL
Match: A0A6J1DRI6 (polygalacturonase-like OS=Momordica charantia OX=3673 GN=LOC111023701 PE=3 SV=1)

HSP 1 Score: 659.1 bits (1699), Expect = 1.2e-185
Identity = 322/391 (82.35%), Postives = 351/391 (89.77%), Query Frame = 0

Query: 1   MANTSSITFFFFFFFLYLLIQTSSAGSFNVISFGAKGDGKTDSTKSFLKAWTSACSSSTP 60
           MAN  S   FFFFFF +L  Q SSAGS+NV+SFGAK DGKTDST+ FLKAW SAC+S T 
Sbjct: 1   MANQMS---FFFFFFFHLFTQRSSAGSYNVLSFGAKPDGKTDSTQPFLKAWKSACTSPTA 60

Query: 61  STINVPKGRFLLTPITFRGPCNNHITFRLNGTLVAPLDYRALGNSGYWILFIKVDGVSFF 120
           STI VPKGRFLLT  TFRGPCN+HITFRLNGTLVAPLDYRALGNSGYWILFIKVDGVS  
Sbjct: 61  STIIVPKGRFLLTSTTFRGPCNSHITFRLNGTLVAPLDYRALGNSGYWILFIKVDGVSVV 120

Query: 121 GGNIDAKGAGYWACKKAGKQCPVGARSLTFNWANNIYVSGITSINSQQTHLVINSCKNVM 180
           GGNIDAKGAGYWACKK+G  CP GARS+TFNWANNI +SG+TSINSQQTHLVINSC NV 
Sbjct: 121 GGNIDAKGAGYWACKKSGNNCPAGARSMTFNWANNIVISGLTSINSQQTHLVINSCNNVK 180

Query: 181 VKNVKVMAPDQSPNTDGIHVQSSFNVTITGSTLQTGDDCISIGDGTKSLLMSHVKCGPGH 240
           VKNVKVMAPDQSPNTDGIHVQSS NV ITGSTLQTGDDCISIGDGTK+L MS++KCGPGH
Sbjct: 181 VKNVKVMAPDQSPNTDGIHVQSSTNVMITGSTLQTGDDCISIGDGTKNLFMSNIKCGPGH 240

Query: 241 GVSIGSLGKEFNEDGVENVTLTNAIFIGSDNGVRIKTWPTASNGFVKNVLFQNIIMKNVK 300
           GVSIGSLGKE  E+GVENVTLTNA+FIGSDNGVRIKTWP  S GFVKNVLFQNIIM NV+
Sbjct: 241 GVSIGSLGKELEENGVENVTLTNAVFIGSDNGVRIKTWPRPSKGFVKNVLFQNIIMNNVQ 300

Query: 301 NPILIDQNYCPNNKGCPSQSSGVKISGVTYRNIQGTSATIEAMTFDCSPSNPCSDIQLQD 360
           NPI+IDQNYCPNN+GCP  SSG+KISGV+Y+NI+GTSAT EA+TFDCSP+ PCSDI+L+D
Sbjct: 301 NPIIIDQNYCPNNQGCPLLSSGLKISGVSYKNIKGTSATPEAVTFDCSPTTPCSDIRLED 360

Query: 361 IKLTYKNKTTTSSCKNIGGSSIGVLVPESCL 392
           IKLTYKNK  TSSCKNIGGS+IG+L+PESCL
Sbjct: 361 IKLTYKNKAATSSCKNIGGSTIGILMPESCL 388

BLAST of Bhi05G000550 vs. ExPASy TrEMBL
Match: A0A0A0M0F3 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G689640 PE=3 SV=1)

HSP 1 Score: 636.3 bits (1640), Expect = 8.2e-179
Identity = 313/391 (80.05%), Postives = 349/391 (89.26%), Query Frame = 0

Query: 1   MAN-TSSITFFFFFFFLYLLIQTSSAGSFNVISFGAKGDGKTDSTKSFLKAWTSACSSST 60
           MAN T S+ F  FF   +L IQ S+A ++NVISFGAK DGKTDST+SFLKAWTSACSSST
Sbjct: 1   MANPTKSLVFIVFF---HLFIQISTAANYNVISFGAKPDGKTDSTQSFLKAWTSACSSST 60

Query: 61  PSTINVPKGRFLLTPITFRGPCNNHITFRLNGTLVAPLDYRALGNSGYWILFIKVDGVSF 120
            STINVPKGRFLLTPITFRGPC N ITF LNGTLVAPLDYRALG+SGYWILFIKVDGVSF
Sbjct: 61  RSTINVPKGRFLLTPITFRGPCKNKITFLLNGTLVAPLDYRALGDSGYWILFIKVDGVSF 120

Query: 121 FGGNIDAKGAGYWACKKAGKQCPVGARSLTFNWANNIYVSGITSINSQQTHLVINSCKNV 180
            GG ID KG  YWACK + K CP GARS+TFNWANNI +SG+TSINSQQTH+VINSC NV
Sbjct: 121 VGGTIDGKGTAYWACKNSAKNCPPGARSITFNWANNIILSGLTSINSQQTHVVINSCNNV 180

Query: 181 MVKNVKVMAPDQSPNTDGIHVQSSFNVTITGSTLQTGDDCISIGDGTKSLLMSHVKCGPG 240
           +VKNVK+MAPDQSPNTDGIHVQSS NVTITGST++TGDDCISIGDGTK+L MS +KCGPG
Sbjct: 181 VVKNVKIMAPDQSPNTDGIHVQSSTNVTITGSTIKTGDDCISIGDGTKNLFMSDIKCGPG 240

Query: 241 HGVSIGSLGKEFNEDGVENVTLTNAIFIGSDNGVRIKTWPTASNGFVKNVLFQNIIMKNV 300
           HGVSIGSLGKE NE+GVENVTL NA+F  SDNGVRIKTWPT SNGFV++V+FQNI+M NV
Sbjct: 241 HGVSIGSLGKEANENGVENVTLINAVFTKSDNGVRIKTWPTPSNGFVRHVIFQNIVMINV 300

Query: 301 KNPILIDQNYCPNNKGCPSQSSGVKISGVTYRNIQGTSATIEAMTFDCSPSNPCSDIQLQ 360
           KNPILIDQNYCPN++ C  QSSGVKI+ VTY++I+GTSAT EA+TFDCS SNPCSDI+L+
Sbjct: 301 KNPILIDQNYCPNHQSCSLQSSGVKINDVTYKSIEGTSATSEAVTFDCSSSNPCSDIKLE 360

Query: 361 DIKLTYKNKTTTSSCKNIGGSSIGVLVPESC 391
           DIKLTYKNKTTTSSCKNIGGSSIG++VPE+C
Sbjct: 361 DIKLTYKNKTTTSSCKNIGGSSIGLVVPETC 388

BLAST of Bhi05G000550 vs. ExPASy TrEMBL
Match: A0A6J1EMF9 (polygalacturonase-like OS=Cucurbita moschata OX=3662 GN=LOC111434691 PE=3 SV=1)

HSP 1 Score: 624.0 bits (1608), Expect = 4.2e-175
Identity = 294/384 (76.56%), Postives = 341/384 (88.80%), Query Frame = 0

Query: 8   TFFFFFFFLYLLIQTSSAGSFNVISFGAKGDGKTDSTKSFLKAWTSACSSSTPSTINVPK 67
           T       +Y  +Q SSA ++NVI++GAK +GKTDST+ FLKAWTSACSSSTPSTINVPK
Sbjct: 7   TLLLIISSIYFFLQLSSAATYNVITYGAKPNGKTDSTRPFLKAWTSACSSSTPSTINVPK 66

Query: 68  GRFLLTPITFRGPCNNHITFRLNGTLVAPLDYRALGNSGYWILFIKVDGVSFFGGNIDAK 127
           GRFLL PITFRGPCNN ITFRLNGTLVAPLDYRALG+SGYWILFIKV+ VSFFGGNIDAK
Sbjct: 67  GRFLLKPITFRGPCNNKITFRLNGTLVAPLDYRALGSSGYWILFIKVNDVSFFGGNIDAK 126

Query: 128 GAGYWACKKAGKQCPVGARSLTFNWANNIYVSGITSINSQQTHLVINSCKNVMVKNVKVM 187
           GA YWAC+ +GK CP GARS+TFNWANNI +SG+TS+NS+Q+H+ INSC NV+VKNVK+M
Sbjct: 127 GASYWACRDSGKDCPPGARSITFNWANNIILSGLTSLNSRQSHITINSCNNVLVKNVKIM 186

Query: 188 APDQSPNTDGIHVQSSFNVTITGSTLQTGDDCISIGDGTKSLLMSHVKCGPGHGVSIGSL 247
           APDQSPNTDGIHVQSS +VTITG+T+QTGDDCISIGDGTK+L M+H+KCGPGHGVSIGSL
Sbjct: 187 APDQSPNTDGIHVQSSTDVTITGTTIQTGDDCISIGDGTKNLFMTHIKCGPGHGVSIGSL 246

Query: 248 GKEFNEDGVENVTLTNAIFIGSDNGVRIKTWPTASNGFVKNVLFQNIIMKNVKNPILIDQ 307
           GKEF+E GV+NVTL NA+F  SDNGVRIKTW    NGFVK+V+FQNI+M NVKNPI+IDQ
Sbjct: 247 GKEFDEQGVQNVTLMNAVFTKSDNGVRIKTWARPGNGFVKHVVFQNIVMNNVKNPIIIDQ 306

Query: 308 NYCPNNKGCPSQSSGVKISGVTYRNIQGTSATIEAMTFDCSPSNPCSDIQLQDIKLTYKN 367
           NYCP+N+GCP QS+GVKIS V Y+NI GTSAT EA+TFDCSP+NPC DI+L+DIKLTY N
Sbjct: 307 NYCPDNEGCPGQSAGVKISDVAYKNIGGTSATSEAITFDCSPTNPCGDIRLEDIKLTYNN 366

Query: 368 KTTTSSCKNIGGSSIGVLVPESCL 392
           K TTS+C N+GGSS+GVL+P+SCL
Sbjct: 367 KATTSTCNNVGGSSMGVLMPQSCL 390

BLAST of Bhi05G000550 vs. ExPASy TrEMBL
Match: A0A6J1JKS6 (polygalacturonase-like OS=Cucurbita maxima OX=3661 GN=LOC111486681 PE=3 SV=1)

HSP 1 Score: 622.5 bits (1604), Expect = 1.2e-174
Identity = 298/391 (76.21%), Postives = 342/391 (87.47%), Query Frame = 0

Query: 1   MANTSSITFFFFFFFLYLLIQTSSAGSFNVISFGAKGDGKTDSTKSFLKAWTSACSSSTP 60
           MANT   T        Y  IQ SSA ++NVI++GAK DGKTDST+ FLKAWTSAC+SSTP
Sbjct: 1   MANTIK-TLLLIISSFYFFIQLSSAATYNVITYGAKPDGKTDSTRPFLKAWTSACTSSTP 60

Query: 61  STINVPKGRFLLTPITFRGPCNNHITFRLNGTLVAPLDYRALGNSGYWILFIKVDGVSFF 120
           STINVPKGRFLL PITFRGPC N ITFRLNGTLVAPLD+RALG+SGYWILFIKV+ VSFF
Sbjct: 61  STINVPKGRFLLKPITFRGPCKNKITFRLNGTLVAPLDFRALGSSGYWILFIKVNDVSFF 120

Query: 121 GGNIDAKGAGYWACKKAGKQCPVGARSLTFNWANNIYVSGITSINSQQTHLVINSCKNVM 180
           GGNIDAKGA YWACK +GK CP GARSLTFNWANNIY+SG+TSINS+Q+H+ INSC NV+
Sbjct: 121 GGNIDAKGASYWACKDSGKDCPPGARSLTFNWANNIYLSGLTSINSRQSHITINSCNNVL 180

Query: 181 VKNVKVMAPDQSPNTDGIHVQSSFNVTITGSTLQTGDDCISIGDGTKSLLMSHVKCGPGH 240
           VKNVK+MAPDQSPNTDGIHVQSS +VTITG+T+QTGDDCISIGDGTK+L M+H+KCGPGH
Sbjct: 181 VKNVKIMAPDQSPNTDGIHVQSSTDVTITGTTIQTGDDCISIGDGTKNLFMAHIKCGPGH 240

Query: 241 GVSIGSLGKEFNEDGVENVTLTNAIFIGSDNGVRIKTWPTASNGFVKNVLFQNIIMKNVK 300
           GVSIGSLGKEF+E GV+NVTL NA+F  SDNGVRIKTW    NGFVK+V+F+NIIM NVK
Sbjct: 241 GVSIGSLGKEFDEQGVQNVTLMNAVFTKSDNGVRIKTWARPGNGFVKHVVFENIIMNNVK 300

Query: 301 NPILIDQNYCPNNKGCPSQSSGVKISGVTYRNIQGTSATIEAMTFDCSPSNPCSDIQLQD 360
           NPI+IDQNYCP+N GCP QS+GVKIS V Y+NI GTSAT EAMTFDCS +NPC +++L+D
Sbjct: 301 NPIIIDQNYCPDNVGCPGQSAGVKISDVRYKNIGGTSATSEAMTFDCSSTNPCDNVRLED 360

Query: 361 IKLTYKNKTTTSSCKNIGGSSIGVLVPESCL 392
           IKLTYKNK +TS+C N+GGSS G+L+P+SCL
Sbjct: 361 IKLTYKNKASTSTCNNVGGSSTGILMPQSCL 390

BLAST of Bhi05G000550 vs. ExPASy TrEMBL
Match: A0A6J1EIK0 (polygalacturonase-like OS=Cucurbita moschata OX=3662 GN=LOC111434690 PE=3 SV=1)

HSP 1 Score: 619.8 bits (1597), Expect = 7.9e-174
Identity = 291/384 (75.78%), Postives = 341/384 (88.80%), Query Frame = 0

Query: 8   TFFFFFFFLYLLIQTSSAGSFNVISFGAKGDGKTDSTKSFLKAWTSACSSSTPSTINVPK 67
           T       +Y  +Q SSA ++NVI++GAK +GKTDST+ FLKAWTSACSSSTPSTINVPK
Sbjct: 7   TLLLIISSIYFFLQLSSAATYNVITYGAKPNGKTDSTRPFLKAWTSACSSSTPSTINVPK 66

Query: 68  GRFLLTPITFRGPCNNHITFRLNGTLVAPLDYRALGNSGYWILFIKVDGVSFFGGNIDAK 127
           GRFLL PITFRGPCNN ITFRLNGTLVAPLDYRALG+SGYWILFIKV+ VSFFGGNIDAK
Sbjct: 67  GRFLLKPITFRGPCNNKITFRLNGTLVAPLDYRALGSSGYWILFIKVNDVSFFGGNIDAK 126

Query: 128 GAGYWACKKAGKQCPVGARSLTFNWANNIYVSGITSINSQQTHLVINSCKNVMVKNVKVM 187
           GA YWAC+ +GK CP GARS+TFNWANNI +SG+TS+NS+Q+H+ INSC NV+VKNVK+M
Sbjct: 127 GASYWACRDSGKDCPPGARSITFNWANNIILSGLTSLNSRQSHITINSCNNVLVKNVKIM 186

Query: 188 APDQSPNTDGIHVQSSFNVTITGSTLQTGDDCISIGDGTKSLLMSHVKCGPGHGVSIGSL 247
           APDQSPNTDGIHVQSS +VTITG+T+QTGDDCISIGDGTK+L M+H+KCGPGHGVSIGSL
Sbjct: 187 APDQSPNTDGIHVQSSTDVTITGTTIQTGDDCISIGDGTKNLFMTHIKCGPGHGVSIGSL 246

Query: 248 GKEFNEDGVENVTLTNAIFIGSDNGVRIKTWPTASNGFVKNVLFQNIIMKNVKNPILIDQ 307
           GKEF+E GV+NVTL NA+F  SDNGVRIKTW    NGFVK+V+FQNI+M NVKNPI+IDQ
Sbjct: 247 GKEFDEQGVQNVTLMNAVFTKSDNGVRIKTWARPGNGFVKHVVFQNIVMNNVKNPIIIDQ 306

Query: 308 NYCPNNKGCPSQSSGVKISGVTYRNIQGTSATIEAMTFDCSPSNPCSDIQLQDIKLTYKN 367
           NYCP+N+GCP QS+GVKIS VTY+NI GTSAT EA+TFDCS +NPC D++L+DIKLTY N
Sbjct: 307 NYCPDNEGCPGQSAGVKISDVTYKNIGGTSATSEAITFDCSSTNPCDDMRLEDIKLTYNN 366

Query: 368 KTTTSSCKNIGGSSIGVLVPESCL 392
           K +TS+C N+GGSS+G+L+P+SCL
Sbjct: 367 KASTSTCNNVGGSSMGILMPQSCL 390

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
AT2G43890.15.3e-15464.54Pectin lyase-like superfamily protein [more]
AT1G05660.11.0e-13359.34Pectin lyase-like superfamily protein [more]
AT1G05650.11.8e-13359.38Pectin lyase-like superfamily protein [more]
AT2G43880.12.4e-13055.58Pectin lyase-like superfamily protein [more]
AT3G59850.12.8e-12356.20Pectin lyase-like superfamily protein [more]
Match NameE-valueIdentityDescription
P489792.0e-11854.71Polygalacturonase OS=Prunus persica OX=3760 PE=2 SV=1[more]
O228181.6e-10251.36Probable polygalacturonase At2g43860 OS=Arabidopsis thaliana OX=3702 GN=At2g4386... [more]
Q397662.1e-8342.78Polygalacturonase OS=Gossypium barbadense OX=3634 GN=G9 PE=2 SV=1[more]
Q397868.1e-8342.53Polygalacturonase OS=Gossypium hirsutum OX=3635 GN=G9 PE=2 SV=1[more]
O231471.7e-7743.32Polygalacturonase ADPG1 OS=Arabidopsis thaliana OX=3702 GN=ADPG1 PE=2 SV=1[more]
Match NameE-valueIdentityDescription
A0A6J1DRI61.2e-18582.35polygalacturonase-like OS=Momordica charantia OX=3673 GN=LOC111023701 PE=3 SV=1[more]
A0A0A0M0F38.2e-17980.05Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G689640 PE=3 SV=1[more]
A0A6J1EMF94.2e-17576.56polygalacturonase-like OS=Cucurbita moschata OX=3662 GN=LOC111434691 PE=3 SV=1[more]
A0A6J1JKS61.2e-17476.21polygalacturonase-like OS=Cucurbita maxima OX=3661 GN=LOC111486681 PE=3 SV=1[more]
A0A6J1EIK07.9e-17475.78polygalacturonase-like OS=Cucurbita moschata OX=3662 GN=LOC111434690 PE=3 SV=1[more]
InterPro
Analysis Name: InterPro Annotations of Wax gourd (B227) v1
Date Performed: 2021-10-22
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR006626Parallel beta-helix repeatSMARTSM00710pbh1coord: 256..277
e-value: 29.0
score: 13.6
coord: 286..307
e-value: 3200.0
score: 3.6
coord: 176..202
e-value: 220.0
score: 10.7
coord: 203..224
e-value: 10.0
score: 15.0
coord: 320..342
e-value: 2300.0
score: 4.6
IPR000743Glycoside hydrolase, family 28PFAMPF00295Glyco_hydro_28coord: 55..378
e-value: 1.1E-91
score: 307.3
IPR000743Glycoside hydrolase, family 28PROSITEPS00502POLYGALACTURONASEcoord: 233..246
IPR012334Pectin lyase foldGENE3D2.160.20.10coord: 18..388
e-value: 3.0E-117
score: 394.1
NoneNo IPR availablePANTHERPTHR31375FAMILY NOT NAMEDcoord: 10..390
NoneNo IPR availablePANTHERPTHR31375:SF31PECTIN LYASE-LIKE SUPERFAMILY PROTEINcoord: 10..390
IPR011050Pectin lyase fold/virulence factorSUPERFAMILY51126Pectin lyase-likecoord: 13..391

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Bhi05M000550Bhi05M000550mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0005975 carbohydrate metabolic process
molecular_function GO:0004650 polygalacturonase activity