Bhi05G000541 (gene) Wax gourd (B227) v1

Overview
NameBhi05G000541
Typegene
OrganismBenincasa hispida (Wax gourd (B227) v1)
DescriptionPyrophosphate--fructose 6-phosphate 1-phosphotransferase subunit beta
Locationchr5: 21759437 .. 21761765 (+)
RNA-Seq ExpressionBhi05G000541
SyntenyBhi05G000541
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
GTTACGGCTAAGAAGTTTACCTTGAAAAACGTTACAGACTACATTGTCGATGTAATTAGTAAACGTTCTGAACTTGGTTATTAATTATGGTGTCGTACTTATTCCCGAGGGTTTGATTGACTTCATTCCGGAGGTATTTCTCTTGTTATTGTAACTAATTGTGTTTATTTCTTTTGGTTGTATGCTCACTATCCACTAAACCCAAGTTCTCTAGATCAATATGTCATTCTTGAACTGATATCATTTATAACCCGCTTTCTACAGGTGCAACAGCTTATTTTGGAACTCAATGAAATTTTTGCCCATGATATTATAGATGAGGATAGGTTATGGAAAAAGAAATATTATTGATGAGGATAGGTTATGGAAAAAGAAACTTACTACTCAATCTCATCACCTTTTTGAATCTTTCCCACAAGCAATCCAAGAACAATTGTTGCTTGAAAGAGATCCACACGACAATGTCCAAGTAATTGTTTTTTCTTTTTGTTTTATACATGTCTTTCCATGAAACTGAGTTGGAAAAGAGAAGGCAAAAAGGTGCATATAATGGGCGGCCAATTCAAGGGGCAGTCACATTTCTTTGGGTACATATTCTATTCATGCTTGAACTTTTGCTGTTTTATTTAAATGCTCCTGTTTAATAATTTATTGATAACACATATTATATTTAGCACTTGGGTAGTAGGCAAATCAATTGAGATCTAAAAAACATGCATGTTGGTAAATGCATGAAGATCTTTTAAATTCATATTTGATAATTAGGGGTATCAAGTATCTAGACTTCCATTATGAACTCGTAAAGAAAATGGCCAATCAAGTGATGGGTTAGGCTTACATCTAATTGACTTTCAAATGTAAACCTACGAATGGGACGAGCGAATGGCCACGAGATGATATGACAAAAATTGTACTTTGCTAGTTAGTGCTAGTGTTATCTCTGTTTATTTCTTCTTTGACTTATATGTACGAAGGGATGTGTGGTTTACCCACGAACTTTGATATCAACTTATTGCTATGCTTTGGGTTACACTGCTGGAGCTCTCCTTCAGAGTGGAAAAACTGGGCCAATTTCATTCTTTTTCTTGATATCAATTTCCTCCATAGATTTTTTCCTAGTTTCTATTACTTACTAGTTATGCATATTAGGTAGGAAATTTGGCTGCTCCAGTTGAAGATTGGATGGCTGGTGGAACTGCATTGACTTCATTAATGGACGTGGAGAGGAGACACGGTATGATTTTTTCATGAAAACGTGCATTCCTGTTCTCATGTAAATCTCACTTTTGTTTGGAACTTGAGTTTTACTGAATGCGTTTGAGGCCAGTTGCTACTTCTTAAGGTTTTAGAGTGTTTGAGTTGTGTGTTATATGTGCGTGCAGATGAGATCACAAAGCTGTTTCCTGATCTTTTCGGCCAACCGTCGGCAATGTTGGTGCCAAGTGATTCCAGTTCGGTGCAGGTGAATAAGAAATTGAAGATCGGAGTTGTCTAGTTTGGTGGCCAAGCACTGAGAGGGCATAATGTGATTTTTGGACTATTTGGTAGGGTCTCATTGTGTCCACATTTATAAATCAAGAATAAATGTTTGCTTTTCTGTTCAAGTTGTGGCTAATTAGCAGCTTCTATGATTTTGGTGAAGATTTCCTACAGGTTCATGCATAAGAAAGCACGTTGTATGGTTTCAAGGGTGTCCTGCAGGGATCATGAAATGTAGAACTGACTGCACAATTTATCTACCCATATCACAATCAGGTTAATTTTCTTGAAGGGATTTCTAGCTTTCACTTGTTAGAATCGTTAGTGTGAATATGTGATTTACACTATGTGAAGTGATTGCCATTTGAATGCAATTCTTGATTTAAATGCATTTTTGTTTTATCAGGGTGGTTTTGATATGATATGCAGTGGGAGGGATCAGATTTGTGCAATATATGCGTGCAGATGAGATCACAAAGTTGTTTTCTGATCTTTTCGGCCAACCGTCGGCAATGTTAGTGCCAAGTGATTCTAATTCGGTGCAGGTGAATAAGAAATTGAAGATTGGAGTTGTCTAGTTTGGTGGCTAAGCACTGGGAGGGCATAATGTGATTTCTGGACTATTTGGTAGGGTCTCATTGTGTCCACATTTATAAATCAAGAATAAATGTTTTCTTTTCTGTTCAAGTTATGGCTAATTAGCAGCTTCTATGATTTTTATGAAGATTACCTATAGGTTCATGCAAAAGGAAGCACGTTGTATGGTTTCAAGGGTGGTCCTGCAGGGATCATGAAATGTAAGTATGTAGAACTAACTGCACAATTGATCTACCCATATCGCAATCAGGTT

mRNA sequence

GTTACGGCTAAGAAGTTTACCTTGAAAAACGTTACAGACTACATTGTCGATGTAGGAAATTTGGCTGCTCCAGTTGAAGATTGGATGGCTGGTGGAACTGCATTGACTTCATTAATGGACGTGGAGAGGAGACACGATGAGATCACAAAGCTGTTTCCTGATCTTTTCGGCCAACCGTCGGCAATGTTGGTGCCAAGTGATTCCAGTTCGGTGCAGGTGAATAAGAAATTGAAGATCGGAGTTGTCTAGTTTGGTGGCCAAGCACTGAGAGGGCATAATGTGATTTTTGGACTATTTGATTACCTATAGGTTCATGCAAAAGGAAGCACGTTGTATGGTTTCAAGGGTGGTCCTGCAGGGATCATGAAATGTAAGTATGTAGAACTAACTGCACAATTGATCTACCCATATCGCAATCAGGTT

Coding sequence (CDS)

GTTACGGCTAAGAAGTTTACCTTGAAAAACGTTACAGACTACATTGTCGATGTAGGAAATTTGGCTGCTCCAGTTGAAGATTGGATGGCTGGTGGAACTGCATTGACTTCATTAATGGACGTGGAGAGGAGACACGATGAGATCACAAAGCTGTTTCCTGATCTTTTCGGCCAACCGTCGGCAATGTTGGTGCCAAGTGATTCCAGTTCGGTGCAGGTGAATAAGAAATTGAAGATCGGAGTTGTCTAG

Protein sequence

VTAKKFTLKNVTDYIVDVGNLAAPVEDWMAGGTALTSLMDVERRHDEITKLFPDLFGQPSAMLVPSDSSSVQVNKKLKIGVV
Homology
BLAST of Bhi05G000541 vs. TAIR 10
Match: AT4G04040.1 (Phosphofructokinase family protein )

HSP 1 Score: 60.8 bits (146), Expect = 5.7e-10
Identity = 32/52 (61.54%), Postives = 35/52 (67.31%), Query Frame = 0

Query: 12  TDYIVDVGNLAAPVEDWMAGGTALTSLMDVERRHDE----ITKLFPDLFGQP 60
           T  I  VGNLAAPVE+W  GGTALTSLMDVERRH +    I K   +L G P
Sbjct: 473 TGLISSVGNLAAPVEEWTVGGTALTSLMDVERRHGKFKPVIKKAMVELEGAP 524

BLAST of Bhi05G000541 vs. TAIR 10
Match: AT1G12000.1 (Phosphofructokinase family protein )

HSP 1 Score: 59.7 bits (143), Expect = 1.3e-09
Identity = 31/52 (59.62%), Postives = 35/52 (67.31%), Query Frame = 0

Query: 12  TDYIVDVGNLAAPVEDWMAGGTALTSLMDVERRHDE----ITKLFPDLFGQP 60
           T  I  VGNLAAPVE+W  GGTALT+LMDVERRH +    I K   +L G P
Sbjct: 471 TGLISSVGNLAAPVEEWTVGGTALTALMDVERRHGKFKPVIKKAMVELEGAP 522

BLAST of Bhi05G000541 vs. ExPASy Swiss-Prot
Match: F4JGR5 (Pyrophosphate--fructose 6-phosphate 1-phosphotransferase subunit beta 2 OS=Arabidopsis thaliana OX=3702 GN=PFP-BETA2 PE=3 SV=1)

HSP 1 Score: 60.8 bits (146), Expect = 8.1e-09
Identity = 32/52 (61.54%), Postives = 35/52 (67.31%), Query Frame = 0

Query: 12  TDYIVDVGNLAAPVEDWMAGGTALTSLMDVERRHDE----ITKLFPDLFGQP 60
           T  I  VGNLAAPVE+W  GGTALTSLMDVERRH +    I K   +L G P
Sbjct: 473 TGLISSVGNLAAPVEEWTVGGTALTSLMDVERRHGKFKPVIKKAMVELEGAP 524

BLAST of Bhi05G000541 vs. ExPASy Swiss-Prot
Match: Q8W4M5 (Pyrophosphate--fructose 6-phosphate 1-phosphotransferase subunit beta 1 OS=Arabidopsis thaliana OX=3702 GN=PFP-BETA1 PE=1 SV=1)

HSP 1 Score: 59.7 bits (143), Expect = 1.8e-08
Identity = 31/52 (59.62%), Postives = 35/52 (67.31%), Query Frame = 0

Query: 12  TDYIVDVGNLAAPVEDWMAGGTALTSLMDVERRHDE----ITKLFPDLFGQP 60
           T  I  VGNLAAPVE+W  GGTALT+LMDVERRH +    I K   +L G P
Sbjct: 471 TGLISSVGNLAAPVEEWTVGGTALTALMDVERRHGKFKPVIKKAMVELEGAP 522

BLAST of Bhi05G000541 vs. ExPASy Swiss-Prot
Match: P21343 (Pyrophosphate--fructose 6-phosphate 1-phosphotransferase subunit beta OS=Solanum tuberosum OX=4113 GN=PFP-BETA PE=1 SV=3)

HSP 1 Score: 59.7 bits (143), Expect = 1.8e-08
Identity = 31/52 (59.62%), Postives = 35/52 (67.31%), Query Frame = 0

Query: 12  TDYIVDVGNLAAPVEDWMAGGTALTSLMDVERRHDE----ITKLFPDLFGQP 60
           T  I  VGNLAAPVE+W  GGTALT+LMDVERRH +    I K   +L G P
Sbjct: 473 TGLISSVGNLAAPVEEWTVGGTALTALMDVERRHGKFKPVIKKAMVELEGAP 524

BLAST of Bhi05G000541 vs. ExPASy Swiss-Prot
Match: Q41141 (Pyrophosphate--fructose 6-phosphate 1-phosphotransferase subunit beta OS=Ricinus communis OX=3988 GN=PFP-BETA PE=3 SV=1)

HSP 1 Score: 53.5 bits (127), Expect = 1.3e-06
Identity = 25/37 (67.57%), Postives = 31/37 (83.78%), Query Frame = 0

Query: 46 DEITKLFPDLFGQPSAMLVPSDSSSVQVNKKLKIGVV 83
          DEI KLFP+LFGQPSAMLVP  + S+  N++LKIG+V
Sbjct: 50 DEIAKLFPNLFGQPSAMLVPDVADSLDSNQQLKIGLV 86

BLAST of Bhi05G000541 vs. ExPASy Swiss-Prot
Match: O83553 (Pyrophosphate--fructose 6-phosphate 1-phosphotransferase OS=Treponema pallidum (strain Nichols) OX=243276 GN=pfp PE=1 SV=1)

HSP 1 Score: 47.4 bits (111), Expect = 9.2e-05
Identity = 25/63 (39.68%), Postives = 32/63 (50.79%), Query Frame = 0

Query: 1   VTAKKFTLKNVTDYIVDVGNLAAPVEDWMAGGTALTSLMDVERRHDE----ITKLFPDLF 60
           +TA    +   T Y+  V NL + V +W  GG  LT LM++ERRH      I K   DL 
Sbjct: 454 LTACLLAVHRFTGYVASVRNLTSSVAEWAVGGVPLTMLMNMERRHGSQKPVIKKALVDLE 513

BLAST of Bhi05G000541 vs. ExPASy TrEMBL
Match: A0A0A0LY96 (Pyrophosphate--fructose 6-phosphate 1-phosphotransferase subunit beta OS=Cucumis sativus OX=3659 GN=PFP-BETA PE=3 SV=1)

HSP 1 Score: 69.3 bits (168), Expect = 8.4e-09
Identity = 34/37 (91.89%), Postives = 35/37 (94.59%), Query Frame = 0

Query: 46  DEITKLFPDLFGQPSAMLVPSDSSSVQVNKKLKIGVV 83
           DEI KLFP L+GQPSAMLVPSDSSSVQVNKKLKIGVV
Sbjct: 66  DEIAKLFPSLYGQPSAMLVPSDSSSVQVNKKLKIGVV 102

BLAST of Bhi05G000541 vs. ExPASy TrEMBL
Match: A0A5D3CMF5 (Pyrophosphate--fructose 6-phosphate 1-phosphotransferase subunit beta OS=Cucumis melo var. makuwa OX=1194695 GN=PFP-BETA PE=3 SV=1)

HSP 1 Score: 66.6 bits (161), Expect = 5.4e-08
Identity = 33/37 (89.19%), Postives = 33/37 (89.19%), Query Frame = 0

Query: 46  DEITKLFPDLFGQPSAMLVPSDSSSVQVNKKLKIGVV 83
           DEI KLFP LFGQPSAMLVPSDS  VQVNKKLKIGVV
Sbjct: 66  DEIAKLFPSLFGQPSAMLVPSDSGYVQVNKKLKIGVV 102

BLAST of Bhi05G000541 vs. ExPASy TrEMBL
Match: A0A1S3CAD2 (Pyrophosphate--fructose 6-phosphate 1-phosphotransferase subunit beta OS=Cucumis melo OX=3656 GN=LOC103498241 PE=3 SV=1)

HSP 1 Score: 66.6 bits (161), Expect = 5.4e-08
Identity = 33/37 (89.19%), Postives = 33/37 (89.19%), Query Frame = 0

Query: 46  DEITKLFPDLFGQPSAMLVPSDSSSVQVNKKLKIGVV 83
           DEI KLFP LFGQPSAMLVPSDS  VQVNKKLKIGVV
Sbjct: 66  DEIAKLFPSLFGQPSAMLVPSDSGYVQVNKKLKIGVV 102

BLAST of Bhi05G000541 vs. ExPASy TrEMBL
Match: A0A6J1KLI1 (Pyrophosphate--fructose 6-phosphate 1-phosphotransferase subunit beta OS=Cucurbita maxima OX=3661 GN=LOC111495656 PE=3 SV=1)

HSP 1 Score: 65.5 bits (158), Expect = 1.2e-07
Identity = 32/37 (86.49%), Postives = 34/37 (91.89%), Query Frame = 0

Query: 46  DEITKLFPDLFGQPSAMLVPSDSSSVQVNKKLKIGVV 83
           DEI KLFP LFGQPSAMLVPSDSS VQ+N+KLKIGVV
Sbjct: 66  DEIAKLFPSLFGQPSAMLVPSDSSLVQMNQKLKIGVV 102

BLAST of Bhi05G000541 vs. ExPASy TrEMBL
Match: A0A6J1EKY9 (Pyrophosphate--fructose 6-phosphate 1-phosphotransferase subunit beta OS=Cucurbita moschata OX=3662 GN=LOC111434254 PE=3 SV=1)

HSP 1 Score: 65.1 bits (157), Expect = 1.6e-07
Identity = 32/37 (86.49%), Postives = 34/37 (91.89%), Query Frame = 0

Query: 46  DEITKLFPDLFGQPSAMLVPSDSSSVQVNKKLKIGVV 83
           DEI KLFP LFGQPSAMLVPSDSS VQ+N+KLKIGVV
Sbjct: 66  DEIAKLFPTLFGQPSAMLVPSDSSLVQMNQKLKIGVV 102

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
AT4G04040.15.7e-1061.54Phosphofructokinase family protein [more]
AT1G12000.11.3e-0959.62Phosphofructokinase family protein [more]
Match NameE-valueIdentityDescription
F4JGR58.1e-0961.54Pyrophosphate--fructose 6-phosphate 1-phosphotransferase subunit beta 2 OS=Arabi... [more]
Q8W4M51.8e-0859.62Pyrophosphate--fructose 6-phosphate 1-phosphotransferase subunit beta 1 OS=Arabi... [more]
P213431.8e-0859.62Pyrophosphate--fructose 6-phosphate 1-phosphotransferase subunit beta OS=Solanum... [more]
Q411411.3e-0667.57Pyrophosphate--fructose 6-phosphate 1-phosphotransferase subunit beta OS=Ricinus... [more]
O835539.2e-0539.68Pyrophosphate--fructose 6-phosphate 1-phosphotransferase OS=Treponema pallidum (... [more]
Match NameE-valueIdentityDescription
A0A0A0LY968.4e-0991.89Pyrophosphate--fructose 6-phosphate 1-phosphotransferase subunit beta OS=Cucumis... [more]
A0A5D3CMF55.4e-0889.19Pyrophosphate--fructose 6-phosphate 1-phosphotransferase subunit beta OS=Cucumis... [more]
A0A1S3CAD25.4e-0889.19Pyrophosphate--fructose 6-phosphate 1-phosphotransferase subunit beta OS=Cucumis... [more]
A0A6J1KLI11.2e-0786.49Pyrophosphate--fructose 6-phosphate 1-phosphotransferase subunit beta OS=Cucurbi... [more]
A0A6J1EKY91.6e-0786.49Pyrophosphate--fructose 6-phosphate 1-phosphotransferase subunit beta OS=Cucurbi... [more]
InterPro
Analysis Name: InterPro Annotations of Wax gourd (B227) v1
Date Performed: 2021-10-22
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableGENE3D3.40.50.450coord: 2..47
e-value: 2.7E-6
score: 28.0
NoneNo IPR availablePANTHERPTHR43650PYROPHOSPHATE--FRUCTOSE 6-PHOSPHATE 1-PHOSPHOTRANSFERASEcoord: 46..82
NoneNo IPR availablePANTHERPTHR43650PYROPHOSPHATE--FRUCTOSE 6-PHOSPHATE 1-PHOSPHOTRANSFERASEcoord: 15..46
NoneNo IPR availablePANTHERPTHR43650:SF16PYROPHOSPHATE--FRUCTOSE 6-PHOSPHATE 1-PHOSPHOTRANSFERASE SUBUNIT BETA 2coord: 46..82
NoneNo IPR availablePANTHERPTHR43650:SF16PYROPHOSPHATE--FRUCTOSE 6-PHOSPHATE 1-PHOSPHOTRANSFERASE SUBUNIT BETA 2coord: 15..46

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Bhi05M000541Bhi05M000541mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0046835 carbohydrate phosphorylation
biological_process GO:0006002 fructose 6-phosphate metabolic process
biological_process GO:0061615 glycolytic process through fructose-6-phosphate
biological_process GO:0015979 photosynthesis
biological_process GO:0009749 response to glucose
cellular_component GO:0005829 cytosol
molecular_function GO:0003872 6-phosphofructokinase activity
molecular_function GO:0005524 ATP binding
molecular_function GO:0047334 diphosphate-fructose-6-phosphate 1-phosphotransferase activity
molecular_function GO:0046872 metal ion binding