Bhi03G000832 (gene) Wax gourd (B227) v1

Overview
NameBhi03G000832
Typegene
OrganismBenincasa hispida (Wax gourd (B227) v1)
DescriptionCysteine desulfurase
Locationchr3: 22083982 .. 22090396 (-)
RNA-Seq ExpressionBhi03G000832
SyntenyBhi03G000832
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
TAAATAGCTGAAAGTCAGGGTAAACCAACGATTCATAAATCACCCGGTTATAAAGTCGCCACGTGTCTACACATGTGGCACAAGGGATTCCATCAGTCGGTGACCCAAACATGGCTGACCCCCTCGGCCTGATCTCCGCTCTCCATTTTGTTGTGCCCTAAATTTCAGAGGGAAGATCTCTCAATGACGAATCTCTACCTTTTCCGTTACTGTATTTAACCATCCAACTCCCTCAAACAAGCCTGTATTCAACGCTACCGCATCCCCTTCAATGGCTTCCAAGATGCTTGCTTCCACTTTACGGCGCACCCTCACAAATTTCTCTCCCGCCGTCTCCCGCTTCCGACCTATCTCCACCGCTGCTGCTGTCGCCGCTTCTCCATCGGAACCCCAAGAAGATGAAACTGGGATTTCTATGAAAGGTGTTAAAATATCTGGACGACCGCTTTATCTTGATATGCAAGCTACATCCCCTGTTGATCCTCGGGTACTAGACGCCATGCTTCCTTATTATCTCTCTCGTTATGGTAATCCACACTCTCGTACGCATCTCTATGGTTGGGAATCGGACCATGCCGTTGAGACGGCTCGTTCCCAAGTTGCTGCTCTTATCGGTGCCTCGCCTAAGGAGATTATTTTTACCTCTGGAGCTACCGAGTCCAATAACATATCCATCAAAGGGGTTATGCACTTCTATAGAGAAAAAAAAAGGCACTTGATTACCACTCAGACCGAGCACAAGTGCGTTCTTGATTCGTGCAGACATTTGCAGCAGGAGGGTTTTGATATTACGTATTTACCGGTTGGGTCCGATGGAATTGTTGATTTGGACAAGCTTCGTTCTGCGATTAGAGCTGACACTGGACTTGTTTCGGTCATGGCGGTTAATAATGAAATTGGTGTCATTCAGCCAGTAGAGGAAATTGGGAAAATTTGCAAGGAATTCGATGTTCCCTTCCATACTGATGCGGCTCAGGCTCTTGGGAAGATTCCAATTGATGTGGAGAAGTGGAATGTTAGCCTGATGTCTTTGAGTGGACATAAGATTTATGGACCGAAGGGTATTGGGGCTTTGTATATGCGGAGGAGGCCGAGGATTCGAGTTGAGCCCCAAATGAATGGAGGTGGACAAGAGAGGGGCATTAGGAGTGGGACTGTGCCAACCCCATTGGTCGTGGGAATGGGTGCAGCCTGTGAGCTTGCTATGAAGGAAATGGAATATGATGATAAGCGAATTACTGCACTGCAGGAACGGCTCTTGAATGGTATAAATGCAAAGCTTGAGGGCGTTGTGGTGAATGGTAGCATGGAGAGGCGCTATCCTGGTAATCTGAATTTGTCGTTTGCGTATGTTGAAGGTGAAAGCTTGCTTATGGGATTGAAGGATGTGGCTGTGTCGAGCGGGAGTGCATGCACCAGCGCCAGTTTGGAGCCTTCATATGTGTTGAGGGCCTTGGGAGTGGATGAGGACATGGCTCACACTTCAATCAGGTTTGGCATTGGGAGATTCACCACGGAGGCTGAGATTGATCGAGCAGTTGAGTTGACAGTACAGCAGGTGGAAAAGTTGAGGGAAATGAGTCCCCTTTATGAAATGGTAAAGGAGGGGATAAACATTAAGGAAATCCAATGGGCTCAACACTGATTGCTTTTATTCTCTGATATAAGATGTCCATTTGCCACTTTTGTGCTTGGATTTGTATGGGATATATGGGTTGTTTGGCAGTAATGGCGGGCCATCTAAGCTATGTTACGTTGAACCATATGGATAGCTTCCAAATTGTCTACAGGTTTTCCTCATCCTCCCTCGCCTAGTGATTGGATGCCCTGAAGAAGGCCCTGAATGGTCCTCAAAAGTTTTAAATAATAACTATGTCAACATTTGCACTATGTGCTTAATATGCAGATAGAGTCCCCCTCCCCTCCCCCACAGCAGGATTGTGATTATGATAAACTCTGAATCTAACAAATGCATTCGTCAGTGGTTTCAATTTTTTTTTTTTTTTTTTTTTTTTTGCCTAATTGCTTTAGTGGAGAGGACATTGATTTTATTAGCTTCTCATATGTCTTAGACCTGTGCGTGGTTTGCTTGAATATTGAGTAAATAAAGTTGAATGATTCTATCTTTGCCACCCAAAAATGCTGCAATAGGCAATATGAGTGGATTCTTGTTAGGAAAATATATTTGACATAGACCTCATGCTGCAATAAGCTGTAGTACATTGTAGGTCAATCTGCCTGAAATTAGTAGTTCGCACAAAATTGAGCTCATTCGGTCACTTCCATATGATCTTTAGAATTAACTGAGCTAGTTTCCATCTCCTCGAATCATTATGCATTTGAGATCTTCCGCAAATAGGTTGGATACCATGTATCCATTATTTAGAACTGTTGGTTAGGTCAAGTTCTATGCTGCTGCTTCGCAGCATACGGTAGTTTCACCATAATGACAACCATCATCTGTCACTTCCTTAAGAAGGTTAATCTTTGCCTCTTTCCTATTTGGTCCACCTGATTCAGCAAAGGACTGTTTAGCTTAATACCTTTTATCTTTTTCTCCCTTCTTGGGAAAATTTGTGGTTTCTCTTCCTTCATTGTTATATAGCTGTTTACATTTCATTCACAACTTTTGTGAGCAAGGCACACAACTTTTTATTAATGACGATAAGATAAGACATCCTTTCGTCCCACTCCCAGCTTGACTAAGATTACAAAAAATTCTTAATCAACAGACAAGAATAATTGTGAAATCATTTAGACAGAGGAATAACCTTCAATTGGCTTAGATATTATAGATTTAAGTACATGCTGTTCATATTATCTGGGGACTAAAGGTTCCACCTCTACCAGTGCAAGATGGATGGGTGGTTTGATGGTACTCACCCTAAGTAATATAACCAGACTTTCTCCAATCTTTCTTTCATGTTGAGGTTTCTGTTGGTGAATGCATAGCATAGATTTAATTTCCATGGCTGGGTATGTTTACATGAGTTCTTTTTGAACGTCATGTGCAGAGGCTCCAGCTTCTAGCTCCATGAATGTCGATGTGCAGCACAACTTTGTTTTGGTTCCTTTTTCTAGTTGTTGGTTCCGTTTGGCATTTTTCTGTTTCTCCCTTTTAGGGAGTTTGTATCCTTGAACTTTTTCTTCCTTATCATATATTGATGGAAAGTTCGTTTCTTGTTAGAGAAAAAAAGTGTAGCACATTTTGAAGTTGCTGAAGAAGATTGCTATAGAAAACAAATGTTGTGTAACTCTTGATATTCTAATAGATTAATGGACAATAGTTTAGTCTTATGGAGCCATGTTACATTGGGGTAAGAGGCTAGTGAAAGTTCTTATAATTTTGGAGACCATGTCCATTTAAAAATTGTTACAATGGCTATTATTAACTTCATATATTTGGAAATTGAAGTTTTGTATGATGAACAGCAACCTTCTGTAAGTAAAATGGCCAGTGAAGTTTTGTATCCATCTACGGGGACACTCTGGATCAAGAAGACTCTGGCTGCATCATTATTAATTTTCTCCCTGCTAGATTAGGACGCTCCTCTTTTTTAATGTAAGTACTTGCTTCCCACAAGGTTTTTCTTTCATTCAAGTTCAAATTCTCAGTACCAGACCTTCACTCTCATGGCCCTCCTAGGCGTATGGGAGGGGGTTAGCCGTGCAAGAGGTAGCCTGTATTAAGCCTTTACTATAGAATGTTGATTTTTAAGATGAAAGTGTTGAAATTTCTGTGCACGGTCATTGACAGAATGACAGCTATAATGAGTGTGAAGAAGCTTAATGTTCCTTAAACTGTGTTTCTGATTGCAGTATTATTGGTTTACCTGAACCTTACACGTTAGGCTTTTATATACGCCTCTATCATGTGATAAAATTAACATTCTAAATCTTCCTAAAAGTATCATCCTAAATCCACATCTTCTGCTGTTCAAAATGAAGTGAAACATTGTAGGTACAACCAATAAGGAGTTTCTAAGGAAAGCTCTTAGTTTGTAGATAATTTTCTTTAAATGTAAATTTATTTATTTATTACAGGATTTTTGGTTGACTTGAATTCACACGTGTTAGGATGTACACACCTAATTCTTCAATTATGTGTTCTAGATCTAACATCAAGTCCATGACTTCTGCAGTCCAAAATGAAATGGAATTTTGTTGGACATTGTTTGAATTTGTGTTCATGCAAAGGTGCAAATGAATCCTTTTCTTCTTAGTGTCTGACTTTCTTACCTAGGAAAGGTTTTTTGCCCTTTGTGGTACTGGAAACAGTGAAGGCTCTCTAGTGCTAGCCACTGGAAAAAGGGTTAGCACTTAAACGGGAAAAAATGAAAGAGATCCTGTGGTGTAGGCGTGCAGTAACGAGTATGAAACCGGGTGTGGTAGGTATAAATTTCAGATTTACAGACGGATAAGAGCAAGACTGAATGGCCAGGTCTATCAAGTCGAACCTACTTGGTCAGTTGAAACCGTGTAGGAGCCCAGGAGGCACAGTTTCGTCTTCTCTATTCTGCATTGGACTTTCACTGTTTAGTAGCACTGTACACAAATGAGAAGCATAAATAAGTGAGTGTTTTAATGTTCCGCTAACAGTTTGCTAATATTAATACAGTTGTCTTGGTATCTGCGTTCTTTTTTATGTTTAAAAGTGAAGATGTCAACAAAACCTCTTTATTCATAGCAGTGTACAACTTATTCTTTGACCATCTTGGGCTACCACCTCCTGCAGAAGACCAAGCATCAAGGAGTTCACATGTTTAAGTCCAAAATATATCAGGAAAATAAGAAAACTTGTCCGCGTCTATGTTTGTACTTATCGATTGTAGCAAAAAGAGGACTTTGGATGAGGTGAGCTGTGAGAGAGCTTTGTGGTAGATTATGGTTGCACGTGGAGACTGTCAGAGGAGGAAGATTGATTTTAGGTTTAATGATAGTTTGGCCGATGCTTGGGGGTAAGCCAGAAGGAATGTTGTTGTAAATCCTTTCTGTTTGTTAGTAATCAGAATGCATGGATGAATTCAAATTCCATTCCTACTAAAATCCTCCTGGCAAACTGAAAGCCCTTTCTTTCTTTCTTTTCTTTCCCCTTCCACTTCTTTTTTCTTTTTTAAGAATGTAGACAACCCTGCCTTAGTTTTGGATGCCCCTCCCATTCATCTGATGATCGTGTAGATTATAATTAACACGAAAATGTAAAGTTGGTTTGAAGAACCAAAAAGAAAATGATCATTTATCTTTATTAATTGGAATTCTGTTAATAGAGGTATGTACAATTTAAAGTTTAGAATTAGTAATCAAGTGTTCTTTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTTTTTTTTGTTTTGTTTTGTTTTTTTTTTTTAATATTTATAGATTTGATAGAGATGTAAACTTCAAAGTTACAAGTACGAACTGCATCATTTAACCTTATACTATCTTCTCTCCTTCATTATCTTTTATTTATTTACTTTTGGGGGTAAAGGAGGAGACAGAGAAAGAAGGGGAGGTAAATTAAAGCGGGTAATTATTTAGATTCTTTTTTTCCCAATTTTGTGTTGGTGGTATGTGGCGGATATTGTTTGGCCGGTTGTTGCTCTGTTTTAAAAGAAGTGCAGAGTGCAGTCTCTGTGCAGCATCAAGTGTACACTGACTTGAACTTTATGCATCCTTAATACTATTTTATTATTTTTTTTTTTTAAAATGCAAGTTTTCATATAGATATGAAAGTTTAAAACTACTTTGATAGCATTATGAGCCCACCCTGGAGACCTGGAAGCAAGCTGTGAAAATCTTCTTGTACCAGATAGAATCAAGAAACCAATGGGTTTCAATTCAAATGGTGGGTGGGTGTGTTTGGTCGAGTTATAACTTCCCTTTCCCTGGGCCTTTAATTGATTGCTGCATTCTTGTTGAAACATGCAGGTATTGTGTAGCAATATCCATTAAATGTCCGTTTGTCTTCATAGAAGAAAGCAACTGCTCAAAAGAAGAGGGGTTTCAGCAGAAGAAGCACCATGTGCAGAACCAGTGGGGAGGTTTTTGGGCAATCTGAATGGAAGAAGCTTTCTTATATGTAAAATACGGTATTCTGATCGAGAAAAGTGATTCTCATTTATGAGATGAGATGATGGCAATATCAGTTGTGGCCGTGGGGGAGAAGGATTATGGGTATGGCCCAGCTGGGAAAGTGAGGTACAAAAATGGGGTTGGGTTGTTTTCAACGCTTTTAGTACCGATGAAAAAGCACAAAAAAGCTTGAGAAAGAAGCCATGGGGAATCAGAATCCTTTTCTTCCCTTTCATCACTGTTCATGAGCTTTGGTTCCCAGCTTTTCAGTTTTGCAATATATGTTTTTTTCTTCTCGATTTTAGCACCGACATTCTCTTTTTCGTGTGAG

mRNA sequence

TAAATAGCTGAAAGTCAGGGTAAACCAACGATTCATAAATCACCCGGTTATAAAGTCGCCACGTGTCTACACATGTGGCACAAGGGATTCCATCAGTCGGTGACCCAAACATGGCTGACCCCCTCGGCCTGATCTCCGCTCTCCATTTTGTTGTGCCCTAAATTTCAGAGGGAAGATCTCTCAATGACGAATCTCTACCTTTTCCGTTACTGTATTTAACCATCCAACTCCCTCAAACAAGCCTGTATTCAACGCTACCGCATCCCCTTCAATGGCTTCCAAGATGCTTGCTTCCACTTTACGGCGCACCCTCACAAATTTCTCTCCCGCCGTCTCCCGCTTCCGACCTATCTCCACCGCTGCTGCTGTCGCCGCTTCTCCATCGGAACCCCAAGAAGATGAAACTGGGATTTCTATGAAAGGTGTTAAAATATCTGGACGACCGCTTTATCTTGATATGCAAGCTACATCCCCTGTTGATCCTCGGGTACTAGACGCCATGCTTCCTTATTATCTCTCTCGTTATGGTAATCCACACTCTCGTACGCATCTCTATGGTTGGGAATCGGACCATGCCGTTGAGACGGCTCGTTCCCAAGTTGCTGCTCTTATCGGTGCCTCGCCTAAGGAGATTATTTTTACCTCTGGAGCTACCGAGTCCAATAACATATCCATCAAAGGGGTTATGCACTTCTATAGAGAAAAAAAAAGGCACTTGATTACCACTCAGACCGAGCACAAGTGCGTTCTTGATTCGTGCAGACATTTGCAGCAGGAGGGTTTTGATATTACGTATTTACCGGTTGGGTCCGATGGAATTGTTGATTTGGACAAGCTTCGTTCTGCGATTAGAGCTGACACTGGACTTGTTTCGGTCATGGCGGTTAATAATGAAATTGGTGTCATTCAGCCAGTAGAGGAAATTGGGAAAATTTGCAAGGAATTCGATGTTCCCTTCCATACTGATGCGGCTCAGGCTCTTGGGAAGATTCCAATTGATGTGGAGAAGTGGAATGTTAGCCTGATGTCTTTGAGTGGACATAAGATTTATGGACCGAAGGGTATTGGGGCTTTGTATATGCGGAGGAGGCCGAGGATTCGAGTTGAGCCCCAAATGAATGGAGGTGGACAAGAGAGGGGCATTAGGAGTGGGACTGTGCCAACCCCATTGGTCGTGGGAATGGGTGCAGCCTGTGAGCTTGCTATGAAGGAAATGGAATATGATGATAAGCGAATTACTGCACTGCAGGAACGGCTCTTGAATGGTATAAATGCAAAGCTTGAGGGCGTTGTGGTGAATGGTAGCATGGAGAGGCGCTATCCTGGTAATCTGAATTTGTCGTTTGCGTATGTTGAAGGTGAAAGCTTGCTTATGGGATTGAAGGATGTGGCTGTGTCGAGCGGGAGTGCATGCACCAGCGCCAGTTTGGAGCCTTCATATGTGTTGAGGGCCTTGGGAGTGGATGAGGACATGGCTCACACTTCAATCAGGTTTGGCATTGGGAGATTCACCACGGAGGCTGAGATTGATCGAGCAGTTGAGTTGACAGTACAGCAGGTGGAAAAGTTGAGGGAAATGAGTCCCCTTTATGAAATGGTAAAGGAGGGGATAAACATTAAGGAAATCCAATGGGCTCAACACTGATTGCTTTTATTCTCTGATATAAGATGTCCATTTGCCACTTTTGTGCTTGGATTTGTATGGGATATATGGGTTGTTTGGCAGTAATGGCGGGCCATCTAAGCTATGTTACGTTGAACCATATGGATAGCTTCCAAATTGTCTACAGGTATTGTGTAGCAATATCCATTAAATGTCCGTTTGTCTTCATAGAAGAAAGCAACTGCTCAAAAGAAGAGGGGTTTCAGCAGAAGAAGCACCATGTGCAGAACCAGTGGGGAGGTTTTTGGGCAATCTGAATGGAAGAAGCTTTCTTATATGTAAAATACGGTATTCTGATCGAGAAAAGTGATTCTCATTTATGAGATGAGATGATGGCAATATCAGTTGTGGCCGTGGGGGAGAAGGATTATGGGTATGGCCCAGCTGGGAAAGTGAGGTACAAAAATGGGGTTGGGTTGTTTTCAACGCTTTTAGTACCGATGAAAAAGCACAAAAAAGCTTGAGAAAGAAGCCATGGGGAATCAGAATCCTTTTCTTCCCTTTCATCACTGTTCATGAGCTTTGGTTCCCAGCTTTTCAGTTTTGCAATATATGTTTTTTTCTTCTCGATTTTAGCACCGACATTCTCTTTTTCGTGTGAG

Coding sequence (CDS)

ATGGCTTCCAAGATGCTTGCTTCCACTTTACGGCGCACCCTCACAAATTTCTCTCCCGCCGTCTCCCGCTTCCGACCTATCTCCACCGCTGCTGCTGTCGCCGCTTCTCCATCGGAACCCCAAGAAGATGAAACTGGGATTTCTATGAAAGGTGTTAAAATATCTGGACGACCGCTTTATCTTGATATGCAAGCTACATCCCCTGTTGATCCTCGGGTACTAGACGCCATGCTTCCTTATTATCTCTCTCGTTATGGTAATCCACACTCTCGTACGCATCTCTATGGTTGGGAATCGGACCATGCCGTTGAGACGGCTCGTTCCCAAGTTGCTGCTCTTATCGGTGCCTCGCCTAAGGAGATTATTTTTACCTCTGGAGCTACCGAGTCCAATAACATATCCATCAAAGGGGTTATGCACTTCTATAGAGAAAAAAAAAGGCACTTGATTACCACTCAGACCGAGCACAAGTGCGTTCTTGATTCGTGCAGACATTTGCAGCAGGAGGGTTTTGATATTACGTATTTACCGGTTGGGTCCGATGGAATTGTTGATTTGGACAAGCTTCGTTCTGCGATTAGAGCTGACACTGGACTTGTTTCGGTCATGGCGGTTAATAATGAAATTGGTGTCATTCAGCCAGTAGAGGAAATTGGGAAAATTTGCAAGGAATTCGATGTTCCCTTCCATACTGATGCGGCTCAGGCTCTTGGGAAGATTCCAATTGATGTGGAGAAGTGGAATGTTAGCCTGATGTCTTTGAGTGGACATAAGATTTATGGACCGAAGGGTATTGGGGCTTTGTATATGCGGAGGAGGCCGAGGATTCGAGTTGAGCCCCAAATGAATGGAGGTGGACAAGAGAGGGGCATTAGGAGTGGGACTGTGCCAACCCCATTGGTCGTGGGAATGGGTGCAGCCTGTGAGCTTGCTATGAAGGAAATGGAATATGATGATAAGCGAATTACTGCACTGCAGGAACGGCTCTTGAATGGTATAAATGCAAAGCTTGAGGGCGTTGTGGTGAATGGTAGCATGGAGAGGCGCTATCCTGGTAATCTGAATTTGTCGTTTGCGTATGTTGAAGGTGAAAGCTTGCTTATGGGATTGAAGGATGTGGCTGTGTCGAGCGGGAGTGCATGCACCAGCGCCAGTTTGGAGCCTTCATATGTGTTGAGGGCCTTGGGAGTGGATGAGGACATGGCTCACACTTCAATCAGGTTTGGCATTGGGAGATTCACCACGGAGGCTGAGATTGATCGAGCAGTTGAGTTGACAGTACAGCAGGTGGAAAAGTTGAGGGAAATGAGTCCCCTTTATGAAATGGTAAAGGAGGGGATAAACATTAAGGAAATCCAATGGGCTCAACACTGA

Protein sequence

MASKMLASTLRRTLTNFSPAVSRFRPISTAAAVAASPSEPQEDETGISMKGVKISGRPLYLDMQATSPVDPRVLDAMLPYYLSRYGNPHSRTHLYGWESDHAVETARSQVAALIGASPKEIIFTSGATESNNISIKGVMHFYREKKRHLITTQTEHKCVLDSCRHLQQEGFDITYLPVGSDGIVDLDKLRSAIRADTGLVSVMAVNNEIGVIQPVEEIGKICKEFDVPFHTDAAQALGKIPIDVEKWNVSLMSLSGHKIYGPKGIGALYMRRRPRIRVEPQMNGGGQERGIRSGTVPTPLVVGMGAACELAMKEMEYDDKRITALQERLLNGINAKLEGVVVNGSMERRYPGNLNLSFAYVEGESLLMGLKDVAVSSGSACTSASLEPSYVLRALGVDEDMAHTSIRFGIGRFTTEAEIDRAVELTVQQVEKLREMSPLYEMVKEGINIKEIQWAQH
Homology
BLAST of Bhi03G000832 vs. TAIR 10
Match: AT5G65720.1 (nitrogen fixation S (NIFS)-like 1 )

HSP 1 Score: 707.6 bits (1825), Expect = 6.5e-204
Identity = 349/457 (76.37%), Postives = 404/457 (88.40%), Query Frame = 0

Query: 1   MASKMLASTLRRTLTNFSPAVSRFRPISTAAAVAASPSEPQEDETGISMKGVKISGRPLY 60
           MASK++++T+RRTLT      SR R +STAAA     +E   ++  I MKGV+ISGRPLY
Sbjct: 1   MASKVISATIRRTLTKPHGTFSRCRYLSTAAAA----TEVNYEDESIMMKGVRISGRPLY 60

Query: 61  LDMQATSPVDPRVLDAMLPYYLSRYGNPHSRTHLYGWESDHAVETARSQVAALIGASPKE 120
           LDMQAT+P+DPRV DAM    +  YGNPHSRTHLYGWE+++AVE AR+QVA LI ASPKE
Sbjct: 61  LDMQATTPIDPRVFDAMNASQIHEYGNPHSRTHLYGWEAENAVENARNQVAKLIEASPKE 120

Query: 121 IIFTSGATESNNISIKGVMHFYREKKRHLITTQTEHKCVLDSCRHLQQEGFDITYLPVGS 180
           I+F SGATE+NN+++KGVMHFY++ K+H+ITTQTEHKCVLDSCRHLQQEGF++TYLPV +
Sbjct: 121 IVFVSGATEANNMAVKGVMHFYKDTKKHVITTQTEHKCVLDSCRHLQQEGFEVTYLPVKT 180

Query: 181 DGIVDLDKLRSAIRADTGLVSVMAVNNEIGVIQPVEEIGKICKEFDVPFHTDAAQALGKI 240
           DG+VDL+ LR AIR DTGLVS+MAVNNEIGV+QP+EEIG ICKE +VPFHTDAAQA+GKI
Sbjct: 181 DGLVDLEMLREAIRPDTGLVSIMAVNNEIGVVQPMEEIGMICKEHNVPFHTDAAQAIGKI 240

Query: 241 PIDVEKWNVSLMSLSGHKIYGPKGIGALYMRRRPRIRVEPQMNGGGQERGIRSGTVPTPL 300
           P+DV+KWNV+LMS+S HKIYGPKG+GALY+RRRPRIR+EP MNGGGQERG+RSGT  T  
Sbjct: 241 PVDVKKWNVALMSMSAHKIYGPKGVGALYVRRRPRIRLEPLMNGGGQERGLRSGTGATQQ 300

Query: 301 VVGMGAACELAMKEMEYDDKRITALQERLLNGINAKLEGVVVNGSMERRYPGNLNLSFAY 360
           +VG GAACELAMKEMEYD+K I  LQERLLNG+  KL+GVVVNGSM+ RY GNLNLSFAY
Sbjct: 301 IVGFGAACELAMKEMEYDEKWIKGLQERLLNGVREKLDGVVVNGSMDSRYVGNLNLSFAY 360

Query: 361 VEGESLLMGLKDVAVSSGSACTSASLEPSYVLRALGVDEDMAHTSIRFGIGRFTTEAEID 420
           VEGESLLMGLK+VAVSSGSACTSASLEPSYVLRALGVDEDMAHTSIRFGIGRFTT+ EID
Sbjct: 361 VEGESLLMGLKEVAVSSGSACTSASLEPSYVLRALGVDEDMAHTSIRFGIGRFTTKEEID 420

Query: 421 RAVELTVQQVEKLREMSPLYEMVKEGINIKEIQWAQH 458
           +AVELTV+QVEKLREMSPLYEMVKEGI+IK IQW+QH
Sbjct: 421 KAVELTVKQVEKLREMSPLYEMVKEGIDIKNIQWSQH 453

BLAST of Bhi03G000832 vs. TAIR 10
Match: AT5G65720.2 (nitrogen fixation S (NIFS)-like 1 )

HSP 1 Score: 553.5 bits (1425), Expect = 1.6e-157
Identity = 266/325 (81.85%), Postives = 302/325 (92.92%), Query Frame = 0

Query: 133 ISIKGVMHFYREKKRHLITTQTEHKCVLDSCRHLQQEGFDITYLPVGSDGIVDLDKLRSA 192
           +++KGVMHFY++ K+H+ITTQTEHKCVLDSCRHLQQEGF++TYLPV +DG+VDL+ LR A
Sbjct: 1   MAVKGVMHFYKDTKKHVITTQTEHKCVLDSCRHLQQEGFEVTYLPVKTDGLVDLEMLREA 60

Query: 193 IRADTGLVSVMAVNNEIGVIQPVEEIGKICKEFDVPFHTDAAQALGKIPIDVEKWNVSLM 252
           IR DTGLVS+MAVNNEIGV+QP+EEIG ICKE +VPFHTDAAQA+GKIP+DV+KWNV+LM
Sbjct: 61  IRPDTGLVSIMAVNNEIGVVQPMEEIGMICKEHNVPFHTDAAQAIGKIPVDVKKWNVALM 120

Query: 253 SLSGHKIYGPKGIGALYMRRRPRIRVEPQMNGGGQERGIRSGTVPTPLVVGMGAACELAM 312
           S+S HKIYGPKG+GALY+RRRPRIR+EP MNGGGQERG+RSGT  T  +VG GAACELAM
Sbjct: 121 SMSAHKIYGPKGVGALYVRRRPRIRLEPLMNGGGQERGLRSGTGATQQIVGFGAACELAM 180

Query: 313 KEMEYDDKRITALQERLLNGINAKLEGVVVNGSMERRYPGNLNLSFAYVEGESLLMGLKD 372
           KEMEYD+K I  LQERLLNG+  KL+GVVVNGSM+ RY GNLNLSFAYVEGESLLMGLK+
Sbjct: 181 KEMEYDEKWIKGLQERLLNGVREKLDGVVVNGSMDSRYVGNLNLSFAYVEGESLLMGLKE 240

Query: 373 VAVSSGSACTSASLEPSYVLRALGVDEDMAHTSIRFGIGRFTTEAEIDRAVELTVQQVEK 432
           VAVSSGSACTSASLEPSYVLRALGVDEDMAHTSIRFGIGRFTT+ EID+AVELTV+QVEK
Sbjct: 241 VAVSSGSACTSASLEPSYVLRALGVDEDMAHTSIRFGIGRFTTKEEIDKAVELTVKQVEK 300

Query: 433 LREMSPLYEMVKEGINIKEIQWAQH 458
           LREMSPLYEMVKEGI+IK IQW+QH
Sbjct: 301 LREMSPLYEMVKEGIDIKNIQWSQH 325

BLAST of Bhi03G000832 vs. TAIR 10
Match: AT1G08490.1 (chloroplastic NIFS-like cysteine desulfurase )

HSP 1 Score: 110.5 bits (275), Expect = 3.5e-24
Identity = 81/272 (29.78%), Postives = 127/272 (46.69%), Query Frame = 0

Query: 27  ISTAAAVAASPSEPQEDETGISM-----KGVKI------SGRPLYLDMQATSPVDPRVLD 86
           +S  +A AAS +    D   +S+     K  +I        + +YLD  ATS     VLD
Sbjct: 31  LSVCSAAAASSATISTDSESVSLGHRVRKDFRILHQEVNGSKLVYLDSAATSQKPAAVLD 90

Query: 87  AMLPYYLSRYGNPHSRTHLYGWESDHAVETARSQVAALIGAS-PKEIIFTSGATES-NNI 146
           A+  YY     N H   H    ++    E AR +VA  I AS  +EI+FT  ATE+ N +
Sbjct: 91  ALQNYYEFYNSNVHRGIHYLSAKATDEFELARKKVARFINASDSREIVFTRNATEAINLV 150

Query: 147 SIKGVMHFYREKKRHLITTQTEHKCVLDSCRHLQQEGFDITYLPVGSDGIVDLDKLRSAI 206
           +    +   +     ++T    H C++      Q+ G  + ++ +  D + D++KLR  I
Sbjct: 151 AYSWGLSNLKPGDEVILTVAEHHSCIVPWQIVSQKTGAVLKFVTLNEDEVPDINKLRELI 210

Query: 207 RADTGLVSVMAVNNEIGVIQPVEEIGKICKEFDVPFHTDAAQALGKIPIDVEKWNVSLMS 266
              T LV+V  V+N +    P+EEI     +       DA Q++  + +DV+K N   + 
Sbjct: 211 SPKTKLVAVHHVSNVLASSLPIEEIVVWAHDVGAKVLVDACQSVPHMVVDVQKLNADFLV 270

Query: 267 LSGHKIYGPKGIGALYMRRRPRIRVEPQMNGG 286
            S HK+ GP GIG LY +      + P + GG
Sbjct: 271 ASSHKMCGPTGIGFLYGKSDLLHSMPPFLGGG 302

BLAST of Bhi03G000832 vs. TAIR 10
Match: AT1G27980.1 (dihydrosphingosine phosphate lyase )

HSP 1 Score: 57.0 bits (136), Expect = 4.6e-08
Identity = 66/255 (25.88%), Postives = 112/255 (43.92%), Query Frame = 0

Query: 108 SQVAALIG----ASPKEII--FTSGATESNNISIKGVMHFYREKK----RHLITTQTEHK 167
           +  AAL+G    AS  +I    TSG TES  +++K    + + KK      +I  ++ H 
Sbjct: 181 AMTAALLGSKETASGGQICGNMTSGGTESIVLAVKSSRDYMKYKKGITRPEMIIPESGHS 240

Query: 168 CVLDSCRHLQQEGFDITYLPVGSDGIVDLDKLRSAIRADTGLVSVMAVNNEIGVIQPVEE 227
               + ++ +     +  +PV  D   D+   R  I  +T ++   A     G+I P+EE
Sbjct: 241 AYDKAAQYFK---IKLWRVPVDKDFRADVKATRRHINRNTIMIVGSAPGFPHGIIDPIEE 300

Query: 228 IGKICKEFDVPFHTDA---------AQALGK--IPIDVEKWNVSLMSLSGHKIYG--PKG 287
           +G++   + + FH D          A+ LG    P D     V+ +S+  HK YG  PKG
Sbjct: 301 LGQLALSYGICFHVDLCLGGFVLPFARKLGYQIPPFDFSVQGVTSISVDVHK-YGLAPKG 360

Query: 288 IGALYMR----RRPRIRVEPQMNGGGQERGIRSGTVPTPLVVGMGAACELAMKEMEY--D 334
              +  R    R+ +     + +GG       +G+ P  LV G  AA  +++ E  Y  +
Sbjct: 361 TSTVLYRNHEIRKHQFVAVTEWSGGLYVSPTIAGSRPGSLVAGAWAAM-MSLGEEGYLQN 420

BLAST of Bhi03G000832 vs. ExPASy Swiss-Prot
Match: O49543 (Cysteine desulfurase, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=NIFS1 PE=1 SV=1)

HSP 1 Score: 707.6 bits (1825), Expect = 9.1e-203
Identity = 349/457 (76.37%), Postives = 404/457 (88.40%), Query Frame = 0

Query: 1   MASKMLASTLRRTLTNFSPAVSRFRPISTAAAVAASPSEPQEDETGISMKGVKISGRPLY 60
           MASK++++T+RRTLT      SR R +STAAA     +E   ++  I MKGV+ISGRPLY
Sbjct: 1   MASKVISATIRRTLTKPHGTFSRCRYLSTAAAA----TEVNYEDESIMMKGVRISGRPLY 60

Query: 61  LDMQATSPVDPRVLDAMLPYYLSRYGNPHSRTHLYGWESDHAVETARSQVAALIGASPKE 120
           LDMQAT+P+DPRV DAM    +  YGNPHSRTHLYGWE+++AVE AR+QVA LI ASPKE
Sbjct: 61  LDMQATTPIDPRVFDAMNASQIHEYGNPHSRTHLYGWEAENAVENARNQVAKLIEASPKE 120

Query: 121 IIFTSGATESNNISIKGVMHFYREKKRHLITTQTEHKCVLDSCRHLQQEGFDITYLPVGS 180
           I+F SGATE+NN+++KGVMHFY++ K+H+ITTQTEHKCVLDSCRHLQQEGF++TYLPV +
Sbjct: 121 IVFVSGATEANNMAVKGVMHFYKDTKKHVITTQTEHKCVLDSCRHLQQEGFEVTYLPVKT 180

Query: 181 DGIVDLDKLRSAIRADTGLVSVMAVNNEIGVIQPVEEIGKICKEFDVPFHTDAAQALGKI 240
           DG+VDL+ LR AIR DTGLVS+MAVNNEIGV+QP+EEIG ICKE +VPFHTDAAQA+GKI
Sbjct: 181 DGLVDLEMLREAIRPDTGLVSIMAVNNEIGVVQPMEEIGMICKEHNVPFHTDAAQAIGKI 240

Query: 241 PIDVEKWNVSLMSLSGHKIYGPKGIGALYMRRRPRIRVEPQMNGGGQERGIRSGTVPTPL 300
           P+DV+KWNV+LMS+S HKIYGPKG+GALY+RRRPRIR+EP MNGGGQERG+RSGT  T  
Sbjct: 241 PVDVKKWNVALMSMSAHKIYGPKGVGALYVRRRPRIRLEPLMNGGGQERGLRSGTGATQQ 300

Query: 301 VVGMGAACELAMKEMEYDDKRITALQERLLNGINAKLEGVVVNGSMERRYPGNLNLSFAY 360
           +VG GAACELAMKEMEYD+K I  LQERLLNG+  KL+GVVVNGSM+ RY GNLNLSFAY
Sbjct: 301 IVGFGAACELAMKEMEYDEKWIKGLQERLLNGVREKLDGVVVNGSMDSRYVGNLNLSFAY 360

Query: 361 VEGESLLMGLKDVAVSSGSACTSASLEPSYVLRALGVDEDMAHTSIRFGIGRFTTEAEID 420
           VEGESLLMGLK+VAVSSGSACTSASLEPSYVLRALGVDEDMAHTSIRFGIGRFTT+ EID
Sbjct: 361 VEGESLLMGLKEVAVSSGSACTSASLEPSYVLRALGVDEDMAHTSIRFGIGRFTTKEEID 420

Query: 421 RAVELTVQQVEKLREMSPLYEMVKEGINIKEIQWAQH 458
           +AVELTV+QVEKLREMSPLYEMVKEGI+IK IQW+QH
Sbjct: 421 KAVELTVKQVEKLREMSPLYEMVKEGIDIKNIQWSQH 453

BLAST of Bhi03G000832 vs. ExPASy Swiss-Prot
Match: Q5RDE7 (Cysteine desulfurase, mitochondrial OS=Pongo abelii OX=9601 GN=NFS1 PE=2 SV=1)

HSP 1 Score: 597.8 bits (1540), Expect = 1.0e-169
Identity = 286/401 (71.32%), Postives = 339/401 (84.54%), Query Frame = 0

Query: 57  RPLYLDMQATSPVDPRVLDAMLPYYLSRYGNPHSRTHLYGWESDHAVETARSQVAALIGA 116
           RPLY+D+QAT+P+DPRVLDAMLPY ++ YGNPHSRTH YGWES+ A+E AR QVA+LIGA
Sbjct: 57  RPLYMDVQATTPLDPRVLDAMLPYLINYYGNPHSRTHAYGWESEAAMERARQQVASLIGA 116

Query: 117 SPKEIIFTSGATESNNISIKGVMHFYREKKRHLITTQTEHKCVLDSCRHLQQEGFDITYL 176
            P+EIIFTSGATESNNI+IKGV  FYR +K+HLITTQTEHKCVLDSCR L+ EGF +TYL
Sbjct: 117 DPREIIFTSGATESNNIAIKGVARFYRSRKKHLITTQTEHKCVLDSCRSLEAEGFQVTYL 176

Query: 177 PVGSDGIVDLDKLRSAIRADTGLVSVMAVNNEIGVIQPVEEIGKICKEFDVPFHTDAAQA 236
           PV   GI+DL +L +AI+ DT LVSVM VNNEIGV QP+ EIG+IC    V FHTDAAQA
Sbjct: 177 PVQKSGIIDLKELEAAIQPDTSLVSVMTVNNEIGVKQPIAEIGRICSSRKVYFHTDAAQA 236

Query: 237 LGKIPIDVEKWNVSLMSLSGHKIYGPKGIGALYMRRRPRIRVEPQMNGGGQERGIRSGTV 296
           +GKIP+DV    + LMS+SGHKIYGPKG+GA+Y+RRRPR+RVE   +GGGQERG+RSGTV
Sbjct: 237 VGKIPLDVNDMKIDLMSISGHKIYGPKGVGAIYIRRRPRVRVEALQSGGGQERGMRSGTV 296

Query: 297 PTPLVVGMGAACELAMKEMEYDDKRITALQERLLNGINAKLEGVVVNGSMERRYPGNLNL 356
           PTPLVVG+GAACE+A +EMEYD KRI+ L ERL+  I   L  VV+NG  E  YPG +NL
Sbjct: 297 PTPLVVGLGAACEVAQQEMEYDHKRISKLSERLIQNIMKSLPDVVMNGDPEHHYPGCINL 356

Query: 357 SFAYVEGESLLMGLKDVAVSSGSACTSASLEPSYVLRALGVDEDMAHTSIRFGIGRFTTE 416
           SFAYVEGESLLM LKDVA+SSGSACTSASLEPSYVLRA+G DED+AH+SIRFG+GRFTTE
Sbjct: 357 SFAYVEGESLLMALKDVALSSGSACTSASLEPSYVLRAIGTDEDLAHSSIRFGVGRFTTE 416

Query: 417 AEIDRAVELTVQQVEKLREMSPLYEMVKEGINIKEIQWAQH 458
            E+D  VE  +Q V++LREMSPL+EMV++GI++K I+W QH
Sbjct: 417 EEVDYTVEKCIQHVKRLREMSPLWEMVQDGIDLKSIKWTQH 457

BLAST of Bhi03G000832 vs. ExPASy Swiss-Prot
Match: Q9Y697 (Cysteine desulfurase, mitochondrial OS=Homo sapiens OX=9606 GN=NFS1 PE=1 SV=3)

HSP 1 Score: 596.7 bits (1537), Expect = 2.3e-169
Identity = 286/401 (71.32%), Postives = 339/401 (84.54%), Query Frame = 0

Query: 57  RPLYLDMQATSPVDPRVLDAMLPYYLSRYGNPHSRTHLYGWESDHAVETARSQVAALIGA 116
           RPLY+D+QAT+P+DPRVLDAMLPY ++ YGNPHSRTH YGWES+ A+E AR QVA+LIGA
Sbjct: 57  RPLYMDVQATTPLDPRVLDAMLPYLINYYGNPHSRTHAYGWESEAAMERARQQVASLIGA 116

Query: 117 SPKEIIFTSGATESNNISIKGVMHFYREKKRHLITTQTEHKCVLDSCRHLQQEGFDITYL 176
            P+EIIFTSGATESNNI+IKGV  FYR +K+HLITTQTEHKCVLDSCR L+ EGF +TYL
Sbjct: 117 DPREIIFTSGATESNNIAIKGVARFYRSRKKHLITTQTEHKCVLDSCRSLEAEGFQVTYL 176

Query: 177 PVGSDGIVDLDKLRSAIRADTGLVSVMAVNNEIGVIQPVEEIGKICKEFDVPFHTDAAQA 236
           PV   GI+DL +L +AI+ DT LVSVM VNNEIGV QP+ EIG+IC    V FHTDAAQA
Sbjct: 177 PVQKSGIIDLKELEAAIQPDTSLVSVMTVNNEIGVKQPIAEIGRICSSRKVYFHTDAAQA 236

Query: 237 LGKIPIDVEKWNVSLMSLSGHKIYGPKGIGALYMRRRPRIRVEPQMNGGGQERGIRSGTV 296
           +GKIP+DV    + LMS+SGHKIYGPKG+GA+Y+RRRPR+RVE   +GGGQERG+RSGTV
Sbjct: 237 VGKIPLDVNDMKIDLMSISGHKIYGPKGVGAIYIRRRPRVRVEALQSGGGQERGMRSGTV 296

Query: 297 PTPLVVGMGAACELAMKEMEYDDKRITALQERLLNGINAKLEGVVVNGSMERRYPGNLNL 356
           PTPLVVG+GAACE+A +EMEYD KRI+ L ERL+  I   L  VV+NG  +  YPG +NL
Sbjct: 297 PTPLVVGLGAACEVAQQEMEYDHKRISKLSERLIQNIMKSLPDVVMNGDPKHHYPGCINL 356

Query: 357 SFAYVEGESLLMGLKDVAVSSGSACTSASLEPSYVLRALGVDEDMAHTSIRFGIGRFTTE 416
           SFAYVEGESLLM LKDVA+SSGSACTSASLEPSYVLRA+G DED+AH+SIRFGIGRFTTE
Sbjct: 357 SFAYVEGESLLMALKDVALSSGSACTSASLEPSYVLRAIGTDEDLAHSSIRFGIGRFTTE 416

Query: 417 AEIDRAVELTVQQVEKLREMSPLYEMVKEGINIKEIQWAQH 458
            E+D  VE  +Q V++LREMSPL+EMV++GI++K I+W QH
Sbjct: 417 EEVDYTVEKCIQHVKRLREMSPLWEMVQDGIDLKSIKWTQH 457

BLAST of Bhi03G000832 vs. ExPASy Swiss-Prot
Match: Q9Z1J3 (Cysteine desulfurase, mitochondrial OS=Mus musculus OX=10090 GN=Nfs1 PE=1 SV=3)

HSP 1 Score: 591.3 bits (1523), Expect = 9.6e-168
Identity = 283/401 (70.57%), Postives = 338/401 (84.29%), Query Frame = 0

Query: 57  RPLYLDMQATSPVDPRVLDAMLPYYLSRYGNPHSRTHLYGWESDHAVETARSQVAALIGA 116
           RPLY+D+QAT+P+DPRVLDAMLPY ++ YGNPHSRTH YGWES+ A+E AR QVA+LIGA
Sbjct: 59  RPLYMDVQATTPLDPRVLDAMLPYLVNYYGNPHSRTHAYGWESEAAMERARQQVASLIGA 118

Query: 117 SPKEIIFTSGATESNNISIKGVMHFYREKKRHLITTQTEHKCVLDSCRHLQQEGFDITYL 176
            P+EIIFTSGATESNNI+IKGV  FYR +K+HL+TTQTEHKCVLDSCR L+ EGF +TYL
Sbjct: 119 DPREIIFTSGATESNNIAIKGVARFYRSRKKHLVTTQTEHKCVLDSCRSLEAEGFRVTYL 178

Query: 177 PVGSDGIVDLDKLRSAIRADTGLVSVMAVNNEIGVIQPVEEIGKICKEFDVPFHTDAAQA 236
           PV   GI+DL +L +AI+ DT LVSVM VNNEIGV QP+ EIG+IC    V FHTDAAQA
Sbjct: 179 PVQKSGIIDLKELEAAIQPDTSLVSVMTVNNEIGVKQPIAEIGQICSSRKVYFHTDAAQA 238

Query: 237 LGKIPIDVEKWNVSLMSLSGHKIYGPKGIGALYMRRRPRIRVEPQMNGGGQERGIRSGTV 296
           +GKIP+DV    + LMS+SGHK+YGPKG+GA+Y+RRRPR+RVE   +GGGQERG+RSGTV
Sbjct: 239 VGKIPLDVNDMKIDLMSISGHKLYGPKGVGAIYIRRRPRVRVEALQSGGGQERGMRSGTV 298

Query: 297 PTPLVVGMGAACELAMKEMEYDDKRITALQERLLNGINAKLEGVVVNGSMERRYPGNLNL 356
           PTPLVVG+GAACELA +EMEYD KRI+ L ERL+  I   L  VV+NG  ++ YPG +NL
Sbjct: 299 PTPLVVGLGAACELAQQEMEYDHKRISKLAERLVQNIMKNLPDVVMNGDPKQHYPGCINL 358

Query: 357 SFAYVEGESLLMGLKDVAVSSGSACTSASLEPSYVLRALGVDEDMAHTSIRFGIGRFTTE 416
           SFAYVEGESLLM LKDVA+SSGSACTSASLEPSYVLRA+G DED+AH+SIRFGIGRFTTE
Sbjct: 359 SFAYVEGESLLMALKDVALSSGSACTSASLEPSYVLRAIGTDEDLAHSSIRFGIGRFTTE 418

Query: 417 AEIDRAVELTVQQVEKLREMSPLYEMVKEGINIKEIQWAQH 458
            E+D   E  +  V++LREMSPL+EMV++GI++K I+W QH
Sbjct: 419 EEVDYTAEKCIHHVKRLREMSPLWEMVQDGIDLKSIKWTQH 459

BLAST of Bhi03G000832 vs. ExPASy Swiss-Prot
Match: Q9VKD3 (Cysteine desulfurase, mitochondrial OS=Drosophila melanogaster OX=7227 GN=Nfs1 PE=1 SV=1)

HSP 1 Score: 589.3 bits (1518), Expect = 3.6e-167
Identity = 294/456 (64.47%), Postives = 359/456 (78.73%), Query Frame = 0

Query: 4   KMLASTLRRTLTNFSPAVSRFRPIS--TAAAVAASPSEPQEDETGISMKGVKISGRPLYL 63
           ++ A  LR   TN    V R    S  TAA  AA+  E +E +   ++K  +  GRPLYL
Sbjct: 7   RIQAQVLRSRATNALANVVRHEATSSRTAAKPAATSKEFRERQVRFNIKNEQTEGRPLYL 66

Query: 64  DMQATSPVDPRVLDAMLPYYLSRYGNPHSRTHLYGWESDHAVETARSQVAALIGASPKEI 123
           D QAT+P+DPRVLDAMLPY  + YGNPHSRTH YGWE++ AVE AR QVA LIGA PKEI
Sbjct: 67  DAQATTPMDPRVLDAMLPYLTNFYGNPHSRTHAYGWETESAVEKAREQVATLIGADPKEI 126

Query: 124 IFTSGATESNNISIKGVMHFYREKKRHLITTQTEHKCVLDSCRHLQQEGFDITYLPVGSD 183
           IFTSGATESNNI++KGV  FY  KKRH+ITTQTEHKCVLDSCR L+ EGF +TYLPV ++
Sbjct: 127 IFTSGATESNNIAVKGVARFYGTKKRHVITTQTEHKCVLDSCRALENEGFKVTYLPVLAN 186

Query: 184 GIVDLDKLRSAIRADTGLVSVMAVNNEIGVIQPVEEIGKICKEFDVPFHTDAAQALGKIP 243
           G++DL +L   I ++T LVS+M VNNEIGV QPV+EIGK+C+   V FHTDAAQA+GK+P
Sbjct: 187 GLIDLQQLEETITSETSLVSIMTVNNEIGVRQPVDEIGKLCRSRRVFFHTDAAQAVGKVP 246

Query: 244 IDVEKWNVSLMSLSGHKIYGPKGIGALYMRRRPRIRVEPQMNGGGQERGIRSGTVPTPLV 303
           +DV   N+ LMS+SGHKIYGPKG+GALY+RRRPR+R+EP  +GGGQERG+RSGTVP PL 
Sbjct: 247 LDVNAMNIDLMSISGHKIYGPKGVGALYVRRRPRVRLEPIQSGGGQERGLRSGTVPAPLA 306

Query: 304 VGMGAACELAMKEMEYDDKRITALQERLLNGINAKLEGVVVNGSMERRYPGNLNLSFAYV 363
           VG+GAA EL+++EM+YD K +  L  RLL+ I++ L  V+ NG  +  Y G LNLSFAYV
Sbjct: 307 VGLGAAAELSLREMDYDKKWVDFLSNRLLDRISSALPHVIRNGDAKATYNGCLNLSFAYV 366

Query: 364 EGESLLMGLKDVAVSSGSACTSASLEPSYVLRALGVDEDMAHTSIRFGIGRFTTEAEIDR 423
           EGESLLM LKDVA+SSGSACTSASLEPSYVLRA+G DED+AH+SIRFGIGRFTT  E+D 
Sbjct: 367 EGESLLMALKDVALSSGSACTSASLEPSYVLRAIGTDEDLAHSSIRFGIGRFTTVEEVDY 426

Query: 424 AVELTVQQVEKLREMSPLYEMVKEGINIKEIQWAQH 458
             +  ++ VE+LREMSPL+EMV+EGI++K IQW+QH
Sbjct: 427 TADKCIKHVERLREMSPLWEMVQEGIDLKTIQWSQH 462

BLAST of Bhi03G000832 vs. NCBI nr
Match: XP_038882447.1 (cysteine desulfurase, mitochondrial [Benincasa hispida])

HSP 1 Score: 899.8 bits (2324), Expect = 9.6e-258
Identity = 457/457 (100.00%), Postives = 457/457 (100.00%), Query Frame = 0

Query: 1   MASKMLASTLRRTLTNFSPAVSRFRPISTAAAVAASPSEPQEDETGISMKGVKISGRPLY 60
           MASKMLASTLRRTLTNFSPAVSRFRPISTAAAVAASPSEPQEDETGISMKGVKISGRPLY
Sbjct: 1   MASKMLASTLRRTLTNFSPAVSRFRPISTAAAVAASPSEPQEDETGISMKGVKISGRPLY 60

Query: 61  LDMQATSPVDPRVLDAMLPYYLSRYGNPHSRTHLYGWESDHAVETARSQVAALIGASPKE 120
           LDMQATSPVDPRVLDAMLPYYLSRYGNPHSRTHLYGWESDHAVETARSQVAALIGASPKE
Sbjct: 61  LDMQATSPVDPRVLDAMLPYYLSRYGNPHSRTHLYGWESDHAVETARSQVAALIGASPKE 120

Query: 121 IIFTSGATESNNISIKGVMHFYREKKRHLITTQTEHKCVLDSCRHLQQEGFDITYLPVGS 180
           IIFTSGATESNNISIKGVMHFYREKKRHLITTQTEHKCVLDSCRHLQQEGFDITYLPVGS
Sbjct: 121 IIFTSGATESNNISIKGVMHFYREKKRHLITTQTEHKCVLDSCRHLQQEGFDITYLPVGS 180

Query: 181 DGIVDLDKLRSAIRADTGLVSVMAVNNEIGVIQPVEEIGKICKEFDVPFHTDAAQALGKI 240
           DGIVDLDKLRSAIRADTGLVSVMAVNNEIGVIQPVEEIGKICKEFDVPFHTDAAQALGKI
Sbjct: 181 DGIVDLDKLRSAIRADTGLVSVMAVNNEIGVIQPVEEIGKICKEFDVPFHTDAAQALGKI 240

Query: 241 PIDVEKWNVSLMSLSGHKIYGPKGIGALYMRRRPRIRVEPQMNGGGQERGIRSGTVPTPL 300
           PIDVEKWNVSLMSLSGHKIYGPKGIGALYMRRRPRIRVEPQMNGGGQERGIRSGTVPTPL
Sbjct: 241 PIDVEKWNVSLMSLSGHKIYGPKGIGALYMRRRPRIRVEPQMNGGGQERGIRSGTVPTPL 300

Query: 301 VVGMGAACELAMKEMEYDDKRITALQERLLNGINAKLEGVVVNGSMERRYPGNLNLSFAY 360
           VVGMGAACELAMKEMEYDDKRITALQERLLNGINAKLEGVVVNGSMERRYPGNLNLSFAY
Sbjct: 301 VVGMGAACELAMKEMEYDDKRITALQERLLNGINAKLEGVVVNGSMERRYPGNLNLSFAY 360

Query: 361 VEGESLLMGLKDVAVSSGSACTSASLEPSYVLRALGVDEDMAHTSIRFGIGRFTTEAEID 420
           VEGESLLMGLKDVAVSSGSACTSASLEPSYVLRALGVDEDMAHTSIRFGIGRFTTEAEID
Sbjct: 361 VEGESLLMGLKDVAVSSGSACTSASLEPSYVLRALGVDEDMAHTSIRFGIGRFTTEAEID 420

Query: 421 RAVELTVQQVEKLREMSPLYEMVKEGINIKEIQWAQH 458
           RAVELTVQQVEKLREMSPLYEMVKEGINIKEIQWAQH
Sbjct: 421 RAVELTVQQVEKLREMSPLYEMVKEGINIKEIQWAQH 457

BLAST of Bhi03G000832 vs. NCBI nr
Match: XP_008440458.1 (PREDICTED: cysteine desulfurase, mitochondrial [Cucumis melo] >KAA0036384.1 cysteine desulfurase [Cucumis melo var. makuwa] >TYK12780.1 cysteine desulfurase [Cucumis melo var. makuwa])

HSP 1 Score: 877.5 bits (2266), Expect = 5.1e-251
Identity = 443/457 (96.94%), Postives = 451/457 (98.69%), Query Frame = 0

Query: 1   MASKMLASTLRRTLTNFSPAVSRFRPISTAAAVAASPSEPQEDETGISMKGVKISGRPLY 60
           MASK+LAS LRRTLTN SP +SRFRPISTAAAVAASPSEP EDETGISMKGVKISGRPLY
Sbjct: 1   MASKILASCLRRTLTNSSPPISRFRPISTAAAVAASPSEPHEDETGISMKGVKISGRPLY 60

Query: 61  LDMQATSPVDPRVLDAMLPYYLSRYGNPHSRTHLYGWESDHAVETARSQVAALIGASPKE 120
           LDMQATSPVDPRVLDAMLPYY+SRYGNPHSRTHLYGWESDHAVETARSQVAALIGASPKE
Sbjct: 61  LDMQATSPVDPRVLDAMLPYYISRYGNPHSRTHLYGWESDHAVETARSQVAALIGASPKE 120

Query: 121 IIFTSGATESNNISIKGVMHFYREKKRHLITTQTEHKCVLDSCRHLQQEGFDITYLPVGS 180
           I+FTSGATESNNISIKGVMHFYREKKRHLITTQTEHKCVLDSCRHLQQEGF+ITYLPVGS
Sbjct: 121 IVFTSGATESNNISIKGVMHFYREKKRHLITTQTEHKCVLDSCRHLQQEGFEITYLPVGS 180

Query: 181 DGIVDLDKLRSAIRADTGLVSVMAVNNEIGVIQPVEEIGKICKEFDVPFHTDAAQALGKI 240
           DGIVDL+KLRSAIR DTGLVSVMAVNNEIGVIQPVEEIGKICKEF+VPFHTDAAQALGKI
Sbjct: 181 DGIVDLEKLRSAIRPDTGLVSVMAVNNEIGVIQPVEEIGKICKEFNVPFHTDAAQALGKI 240

Query: 241 PIDVEKWNVSLMSLSGHKIYGPKGIGALYMRRRPRIRVEPQMNGGGQERGIRSGTVPTPL 300
           PIDVEKWNVSLMSLSGHKIYGPKGIGALYMRRRPRIRVEPQMNGGGQERGIRSGTVPTPL
Sbjct: 241 PIDVEKWNVSLMSLSGHKIYGPKGIGALYMRRRPRIRVEPQMNGGGQERGIRSGTVPTPL 300

Query: 301 VVGMGAACELAMKEMEYDDKRITALQERLLNGINAKLEGVVVNGSMERRYPGNLNLSFAY 360
           VVGMGAACELAMKEMEYDDKRITALQERLLNGINAKLEGVVVNGSME+RYPGNLNLSFAY
Sbjct: 301 VVGMGAACELAMKEMEYDDKRITALQERLLNGINAKLEGVVVNGSMEKRYPGNLNLSFAY 360

Query: 361 VEGESLLMGLKDVAVSSGSACTSASLEPSYVLRALGVDEDMAHTSIRFGIGRFTTEAEID 420
           VEGESLLMGLKDVAVSSGSACTSASLEPSYVLRALGVDEDMAHTSIRFGIGRFTTEAEID
Sbjct: 361 VEGESLLMGLKDVAVSSGSACTSASLEPSYVLRALGVDEDMAHTSIRFGIGRFTTEAEID 420

Query: 421 RAVELTVQQVEKLREMSPLYEMVKEGINIKEIQWAQH 458
           RAVELTV QVEKLREMSPLYEMVKEGINIKEIQWAQH
Sbjct: 421 RAVELTVHQVEKLREMSPLYEMVKEGINIKEIQWAQH 457

BLAST of Bhi03G000832 vs. NCBI nr
Match: XP_011657914.1 (cysteine desulfurase, mitochondrial [Cucumis sativus])

HSP 1 Score: 868.6 bits (2243), Expect = 2.4e-248
Identity = 441/458 (96.29%), Postives = 450/458 (98.25%), Query Frame = 0

Query: 1   MASKMLASTLRRTLTNFSPAVSRFRPIST-AAAVAASPSEPQEDETGISMKGVKISGRPL 60
           MASK+LASTLRRTL+N S  +SRFRPIST AAAVAASPSEP EDETGISMKGVKISGRPL
Sbjct: 1   MASKILASTLRRTLSNSSSPISRFRPISTAAAAVAASPSEPHEDETGISMKGVKISGRPL 60

Query: 61  YLDMQATSPVDPRVLDAMLPYYLSRYGNPHSRTHLYGWESDHAVETARSQVAALIGASPK 120
           YLDMQATSPVDPRVLDAMLPYY+SRYGNPHSRTHLYGWESDHAVETARSQVAALIGASPK
Sbjct: 61  YLDMQATSPVDPRVLDAMLPYYISRYGNPHSRTHLYGWESDHAVETARSQVAALIGASPK 120

Query: 121 EIIFTSGATESNNISIKGVMHFYREKKRHLITTQTEHKCVLDSCRHLQQEGFDITYLPVG 180
           EI+FTSGATESNNISIKGVMHFYR+KKRHLITTQTEHKCVLDSCRHLQQEGF+ITYLPVG
Sbjct: 121 EIVFTSGATESNNISIKGVMHFYRDKKRHLITTQTEHKCVLDSCRHLQQEGFEITYLPVG 180

Query: 181 SDGIVDLDKLRSAIRADTGLVSVMAVNNEIGVIQPVEEIGKICKEFDVPFHTDAAQALGK 240
           SDGIVDL+KLRSAIR DTGLVSVMAVNNEIGVIQPVEEIGKICKEF+VPFHTDAAQALGK
Sbjct: 181 SDGIVDLEKLRSAIRPDTGLVSVMAVNNEIGVIQPVEEIGKICKEFNVPFHTDAAQALGK 240

Query: 241 IPIDVEKWNVSLMSLSGHKIYGPKGIGALYMRRRPRIRVEPQMNGGGQERGIRSGTVPTP 300
           IPIDVEKWNVSLMSLSGHKIYGPKGIGALYMRRRPRIRVEPQMNGGGQERGIRSGTVPTP
Sbjct: 241 IPIDVEKWNVSLMSLSGHKIYGPKGIGALYMRRRPRIRVEPQMNGGGQERGIRSGTVPTP 300

Query: 301 LVVGMGAACELAMKEMEYDDKRITALQERLLNGINAKLEGVVVNGSMERRYPGNLNLSFA 360
           LVVGMGAACELAMKEMEYDDKRITALQERLLNGINAKLEGVVVNGSME RYPGNLNLSFA
Sbjct: 301 LVVGMGAACELAMKEMEYDDKRITALQERLLNGINAKLEGVVVNGSMENRYPGNLNLSFA 360

Query: 361 YVEGESLLMGLKDVAVSSGSACTSASLEPSYVLRALGVDEDMAHTSIRFGIGRFTTEAEI 420
           YVEGESLLMGLKDVAVSSGSACTSASLEPSYVLRALGVDEDMAHTSIRFGIGRFTTEAEI
Sbjct: 361 YVEGESLLMGLKDVAVSSGSACTSASLEPSYVLRALGVDEDMAHTSIRFGIGRFTTEAEI 420

Query: 421 DRAVELTVQQVEKLREMSPLYEMVKEGINIKEIQWAQH 458
           DRAVELTV QVEKLREMSPLYEMVKEGINIKEIQWAQH
Sbjct: 421 DRAVELTVHQVEKLREMSPLYEMVKEGINIKEIQWAQH 458

BLAST of Bhi03G000832 vs. NCBI nr
Match: XP_023518255.1 (cysteine desulfurase, mitochondrial [Cucurbita pepo subsp. pepo])

HSP 1 Score: 862.4 bits (2227), Expect = 1.7e-246
Identity = 434/457 (94.97%), Postives = 448/457 (98.03%), Query Frame = 0

Query: 1   MASKMLASTLRRTLTNFSPAVSRFRPISTAAAVAASPSEPQEDETGISMKGVKISGRPLY 60
           MASK+LAS LRRTLT FSPAV R R ISTAAAVA SPSE  EDE+GISMKGVKISGRPLY
Sbjct: 1   MASKILASGLRRTLTEFSPAVLRLRHISTAAAVATSPSELHEDESGISMKGVKISGRPLY 60

Query: 61  LDMQATSPVDPRVLDAMLPYYLSRYGNPHSRTHLYGWESDHAVETARSQVAALIGASPKE 120
           LDMQATSPVDPRVLDAMLPYYLSRYGNPHSRTHLYGWESDHAVETARSQVAALIGASPKE
Sbjct: 61  LDMQATSPVDPRVLDAMLPYYLSRYGNPHSRTHLYGWESDHAVETARSQVAALIGASPKE 120

Query: 121 IIFTSGATESNNISIKGVMHFYREKKRHLITTQTEHKCVLDSCRHLQQEGFDITYLPVGS 180
           I+FTSGATESNNIS+KGVMHFY+EKK+H+ITTQTEHKCVLDSCRHLQQEGFDITYLPVG+
Sbjct: 121 IVFTSGATESNNISVKGVMHFYKEKKKHVITTQTEHKCVLDSCRHLQQEGFDITYLPVGT 180

Query: 181 DGIVDLDKLRSAIRADTGLVSVMAVNNEIGVIQPVEEIGKICKEFDVPFHTDAAQALGKI 240
           DGIVDL+KLRSAIR DTGLVS+MAVNNEIGVIQPVEEIGKICKEF+VPFHTDAAQALGKI
Sbjct: 181 DGIVDLEKLRSAIRPDTGLVSIMAVNNEIGVIQPVEEIGKICKEFNVPFHTDAAQALGKI 240

Query: 241 PIDVEKWNVSLMSLSGHKIYGPKGIGALYMRRRPRIRVEPQMNGGGQERGIRSGTVPTPL 300
           PIDVEKWNVSLMSLSGHKIYGPKGIGALY+RRRPRIRVEPQMNGGGQERGIRSGTVPTPL
Sbjct: 241 PIDVEKWNVSLMSLSGHKIYGPKGIGALYLRRRPRIRVEPQMNGGGQERGIRSGTVPTPL 300

Query: 301 VVGMGAACELAMKEMEYDDKRITALQERLLNGINAKLEGVVVNGSMERRYPGNLNLSFAY 360
           VVGMGAACELAMKEMEYDDKRITALQERLLNG+NAKLEGVVVNGSME+RYPGNLNLSFAY
Sbjct: 301 VVGMGAACELAMKEMEYDDKRITALQERLLNGVNAKLEGVVVNGSMEKRYPGNLNLSFAY 360

Query: 361 VEGESLLMGLKDVAVSSGSACTSASLEPSYVLRALGVDEDMAHTSIRFGIGRFTTEAEID 420
           VEGESLLMGLKDVAVSSGSACTSASLEPSYVLRALGVDEDMAHTSIRFGIGRFTTEAEID
Sbjct: 361 VEGESLLMGLKDVAVSSGSACTSASLEPSYVLRALGVDEDMAHTSIRFGIGRFTTEAEID 420

Query: 421 RAVELTVQQVEKLREMSPLYEMVKEGINIKEIQWAQH 458
           RAVELTVQQVEKLREMSPLYEMVKEGINIKEIQWAQH
Sbjct: 421 RAVELTVQQVEKLREMSPLYEMVKEGINIKEIQWAQH 457

BLAST of Bhi03G000832 vs. NCBI nr
Match: XP_022132842.1 (cysteine desulfurase, mitochondrial [Momordica charantia])

HSP 1 Score: 861.3 bits (2224), Expect = 3.8e-246
Identity = 433/457 (94.75%), Postives = 445/457 (97.37%), Query Frame = 0

Query: 1   MASKMLASTLRRTLTNFSPAVSRFRPISTAAAVAASPSEPQEDETGISMKGVKISGRPLY 60
           MASK+LAS+LRRTLT  + A S  RP+STAAAV  SPSEP EDETGISMKGVKISGRPLY
Sbjct: 1   MASKILASSLRRTLTKSTQAASLLRPLSTAAAVVTSPSEPHEDETGISMKGVKISGRPLY 60

Query: 61  LDMQATSPVDPRVLDAMLPYYLSRYGNPHSRTHLYGWESDHAVETARSQVAALIGASPKE 120
           LDMQATSPVDPRVLDAMLPYYLSRYGNPHSRTHLYGWESDHAVETARSQVAALIGASPKE
Sbjct: 61  LDMQATSPVDPRVLDAMLPYYLSRYGNPHSRTHLYGWESDHAVETARSQVAALIGASPKE 120

Query: 121 IIFTSGATESNNISIKGVMHFYREKKRHLITTQTEHKCVLDSCRHLQQEGFDITYLPVGS 180
           I+FTSGATESNNIS+KGVMHFYREKKRHLITTQTEHKCVLDSCRHLQQEGFDITYLPVGS
Sbjct: 121 IVFTSGATESNNISVKGVMHFYREKKRHLITTQTEHKCVLDSCRHLQQEGFDITYLPVGS 180

Query: 181 DGIVDLDKLRSAIRADTGLVSVMAVNNEIGVIQPVEEIGKICKEFDVPFHTDAAQALGKI 240
           DGIVDL+KLRSAIR +TGLVSVMAVNNEIGVIQP+EEIGKICKEF VPFHTDAAQALGKI
Sbjct: 181 DGIVDLEKLRSAIRPETGLVSVMAVNNEIGVIQPMEEIGKICKEFGVPFHTDAAQALGKI 240

Query: 241 PIDVEKWNVSLMSLSGHKIYGPKGIGALYMRRRPRIRVEPQMNGGGQERGIRSGTVPTPL 300
           PIDVEKWNVSLMSLSGHK+YGPKGIGALY+RRRPRIRVEPQMNGGGQERGIRSGTVPTPL
Sbjct: 241 PIDVEKWNVSLMSLSGHKVYGPKGIGALYLRRRPRIRVEPQMNGGGQERGIRSGTVPTPL 300

Query: 301 VVGMGAACELAMKEMEYDDKRITALQERLLNGINAKLEGVVVNGSMERRYPGNLNLSFAY 360
           VVGMGAACELAMKEMEYDDKRITALQERLLNGINAKLEGVVVNGSMERRYPGNLNLSFAY
Sbjct: 301 VVGMGAACELAMKEMEYDDKRITALQERLLNGINAKLEGVVVNGSMERRYPGNLNLSFAY 360

Query: 361 VEGESLLMGLKDVAVSSGSACTSASLEPSYVLRALGVDEDMAHTSIRFGIGRFTTEAEID 420
           VEGESLLMGLKDVAVSSGSACTSASLEPSYVLRALGVDEDMAHTSIRFGIGRFTTEAEID
Sbjct: 361 VEGESLLMGLKDVAVSSGSACTSASLEPSYVLRALGVDEDMAHTSIRFGIGRFTTEAEID 420

Query: 421 RAVELTVQQVEKLREMSPLYEMVKEGINIKEIQWAQH 458
           RA+ELTV QVEKLREMSPLYEMVKEGINIKEIQWAQH
Sbjct: 421 RAIELTVHQVEKLREMSPLYEMVKEGINIKEIQWAQH 457

BLAST of Bhi03G000832 vs. ExPASy TrEMBL
Match: A0A5D3CMX0 (Cysteine desulfurase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold255G003690 PE=3 SV=1)

HSP 1 Score: 877.5 bits (2266), Expect = 2.5e-251
Identity = 443/457 (96.94%), Postives = 451/457 (98.69%), Query Frame = 0

Query: 1   MASKMLASTLRRTLTNFSPAVSRFRPISTAAAVAASPSEPQEDETGISMKGVKISGRPLY 60
           MASK+LAS LRRTLTN SP +SRFRPISTAAAVAASPSEP EDETGISMKGVKISGRPLY
Sbjct: 1   MASKILASCLRRTLTNSSPPISRFRPISTAAAVAASPSEPHEDETGISMKGVKISGRPLY 60

Query: 61  LDMQATSPVDPRVLDAMLPYYLSRYGNPHSRTHLYGWESDHAVETARSQVAALIGASPKE 120
           LDMQATSPVDPRVLDAMLPYY+SRYGNPHSRTHLYGWESDHAVETARSQVAALIGASPKE
Sbjct: 61  LDMQATSPVDPRVLDAMLPYYISRYGNPHSRTHLYGWESDHAVETARSQVAALIGASPKE 120

Query: 121 IIFTSGATESNNISIKGVMHFYREKKRHLITTQTEHKCVLDSCRHLQQEGFDITYLPVGS 180
           I+FTSGATESNNISIKGVMHFYREKKRHLITTQTEHKCVLDSCRHLQQEGF+ITYLPVGS
Sbjct: 121 IVFTSGATESNNISIKGVMHFYREKKRHLITTQTEHKCVLDSCRHLQQEGFEITYLPVGS 180

Query: 181 DGIVDLDKLRSAIRADTGLVSVMAVNNEIGVIQPVEEIGKICKEFDVPFHTDAAQALGKI 240
           DGIVDL+KLRSAIR DTGLVSVMAVNNEIGVIQPVEEIGKICKEF+VPFHTDAAQALGKI
Sbjct: 181 DGIVDLEKLRSAIRPDTGLVSVMAVNNEIGVIQPVEEIGKICKEFNVPFHTDAAQALGKI 240

Query: 241 PIDVEKWNVSLMSLSGHKIYGPKGIGALYMRRRPRIRVEPQMNGGGQERGIRSGTVPTPL 300
           PIDVEKWNVSLMSLSGHKIYGPKGIGALYMRRRPRIRVEPQMNGGGQERGIRSGTVPTPL
Sbjct: 241 PIDVEKWNVSLMSLSGHKIYGPKGIGALYMRRRPRIRVEPQMNGGGQERGIRSGTVPTPL 300

Query: 301 VVGMGAACELAMKEMEYDDKRITALQERLLNGINAKLEGVVVNGSMERRYPGNLNLSFAY 360
           VVGMGAACELAMKEMEYDDKRITALQERLLNGINAKLEGVVVNGSME+RYPGNLNLSFAY
Sbjct: 301 VVGMGAACELAMKEMEYDDKRITALQERLLNGINAKLEGVVVNGSMEKRYPGNLNLSFAY 360

Query: 361 VEGESLLMGLKDVAVSSGSACTSASLEPSYVLRALGVDEDMAHTSIRFGIGRFTTEAEID 420
           VEGESLLMGLKDVAVSSGSACTSASLEPSYVLRALGVDEDMAHTSIRFGIGRFTTEAEID
Sbjct: 361 VEGESLLMGLKDVAVSSGSACTSASLEPSYVLRALGVDEDMAHTSIRFGIGRFTTEAEID 420

Query: 421 RAVELTVQQVEKLREMSPLYEMVKEGINIKEIQWAQH 458
           RAVELTV QVEKLREMSPLYEMVKEGINIKEIQWAQH
Sbjct: 421 RAVELTVHQVEKLREMSPLYEMVKEGINIKEIQWAQH 457

BLAST of Bhi03G000832 vs. ExPASy TrEMBL
Match: A0A1S3B0Q9 (Cysteine desulfurase OS=Cucumis melo OX=3656 GN=LOC103484889 PE=3 SV=1)

HSP 1 Score: 877.5 bits (2266), Expect = 2.5e-251
Identity = 443/457 (96.94%), Postives = 451/457 (98.69%), Query Frame = 0

Query: 1   MASKMLASTLRRTLTNFSPAVSRFRPISTAAAVAASPSEPQEDETGISMKGVKISGRPLY 60
           MASK+LAS LRRTLTN SP +SRFRPISTAAAVAASPSEP EDETGISMKGVKISGRPLY
Sbjct: 1   MASKILASCLRRTLTNSSPPISRFRPISTAAAVAASPSEPHEDETGISMKGVKISGRPLY 60

Query: 61  LDMQATSPVDPRVLDAMLPYYLSRYGNPHSRTHLYGWESDHAVETARSQVAALIGASPKE 120
           LDMQATSPVDPRVLDAMLPYY+SRYGNPHSRTHLYGWESDHAVETARSQVAALIGASPKE
Sbjct: 61  LDMQATSPVDPRVLDAMLPYYISRYGNPHSRTHLYGWESDHAVETARSQVAALIGASPKE 120

Query: 121 IIFTSGATESNNISIKGVMHFYREKKRHLITTQTEHKCVLDSCRHLQQEGFDITYLPVGS 180
           I+FTSGATESNNISIKGVMHFYREKKRHLITTQTEHKCVLDSCRHLQQEGF+ITYLPVGS
Sbjct: 121 IVFTSGATESNNISIKGVMHFYREKKRHLITTQTEHKCVLDSCRHLQQEGFEITYLPVGS 180

Query: 181 DGIVDLDKLRSAIRADTGLVSVMAVNNEIGVIQPVEEIGKICKEFDVPFHTDAAQALGKI 240
           DGIVDL+KLRSAIR DTGLVSVMAVNNEIGVIQPVEEIGKICKEF+VPFHTDAAQALGKI
Sbjct: 181 DGIVDLEKLRSAIRPDTGLVSVMAVNNEIGVIQPVEEIGKICKEFNVPFHTDAAQALGKI 240

Query: 241 PIDVEKWNVSLMSLSGHKIYGPKGIGALYMRRRPRIRVEPQMNGGGQERGIRSGTVPTPL 300
           PIDVEKWNVSLMSLSGHKIYGPKGIGALYMRRRPRIRVEPQMNGGGQERGIRSGTVPTPL
Sbjct: 241 PIDVEKWNVSLMSLSGHKIYGPKGIGALYMRRRPRIRVEPQMNGGGQERGIRSGTVPTPL 300

Query: 301 VVGMGAACELAMKEMEYDDKRITALQERLLNGINAKLEGVVVNGSMERRYPGNLNLSFAY 360
           VVGMGAACELAMKEMEYDDKRITALQERLLNGINAKLEGVVVNGSME+RYPGNLNLSFAY
Sbjct: 301 VVGMGAACELAMKEMEYDDKRITALQERLLNGINAKLEGVVVNGSMEKRYPGNLNLSFAY 360

Query: 361 VEGESLLMGLKDVAVSSGSACTSASLEPSYVLRALGVDEDMAHTSIRFGIGRFTTEAEID 420
           VEGESLLMGLKDVAVSSGSACTSASLEPSYVLRALGVDEDMAHTSIRFGIGRFTTEAEID
Sbjct: 361 VEGESLLMGLKDVAVSSGSACTSASLEPSYVLRALGVDEDMAHTSIRFGIGRFTTEAEID 420

Query: 421 RAVELTVQQVEKLREMSPLYEMVKEGINIKEIQWAQH 458
           RAVELTV QVEKLREMSPLYEMVKEGINIKEIQWAQH
Sbjct: 421 RAVELTVHQVEKLREMSPLYEMVKEGINIKEIQWAQH 457

BLAST of Bhi03G000832 vs. ExPASy TrEMBL
Match: A0A0A0KIM0 (Cysteine desulfurase OS=Cucumis sativus OX=3659 GN=Csa_6G497010 PE=3 SV=1)

HSP 1 Score: 868.6 bits (2243), Expect = 1.1e-248
Identity = 441/458 (96.29%), Postives = 450/458 (98.25%), Query Frame = 0

Query: 1   MASKMLASTLRRTLTNFSPAVSRFRPIST-AAAVAASPSEPQEDETGISMKGVKISGRPL 60
           MASK+LASTLRRTL+N S  +SRFRPIST AAAVAASPSEP EDETGISMKGVKISGRPL
Sbjct: 1   MASKILASTLRRTLSNSSSPISRFRPISTAAAAVAASPSEPHEDETGISMKGVKISGRPL 60

Query: 61  YLDMQATSPVDPRVLDAMLPYYLSRYGNPHSRTHLYGWESDHAVETARSQVAALIGASPK 120
           YLDMQATSPVDPRVLDAMLPYY+SRYGNPHSRTHLYGWESDHAVETARSQVAALIGASPK
Sbjct: 61  YLDMQATSPVDPRVLDAMLPYYISRYGNPHSRTHLYGWESDHAVETARSQVAALIGASPK 120

Query: 121 EIIFTSGATESNNISIKGVMHFYREKKRHLITTQTEHKCVLDSCRHLQQEGFDITYLPVG 180
           EI+FTSGATESNNISIKGVMHFYR+KKRHLITTQTEHKCVLDSCRHLQQEGF+ITYLPVG
Sbjct: 121 EIVFTSGATESNNISIKGVMHFYRDKKRHLITTQTEHKCVLDSCRHLQQEGFEITYLPVG 180

Query: 181 SDGIVDLDKLRSAIRADTGLVSVMAVNNEIGVIQPVEEIGKICKEFDVPFHTDAAQALGK 240
           SDGIVDL+KLRSAIR DTGLVSVMAVNNEIGVIQPVEEIGKICKEF+VPFHTDAAQALGK
Sbjct: 181 SDGIVDLEKLRSAIRPDTGLVSVMAVNNEIGVIQPVEEIGKICKEFNVPFHTDAAQALGK 240

Query: 241 IPIDVEKWNVSLMSLSGHKIYGPKGIGALYMRRRPRIRVEPQMNGGGQERGIRSGTVPTP 300
           IPIDVEKWNVSLMSLSGHKIYGPKGIGALYMRRRPRIRVEPQMNGGGQERGIRSGTVPTP
Sbjct: 241 IPIDVEKWNVSLMSLSGHKIYGPKGIGALYMRRRPRIRVEPQMNGGGQERGIRSGTVPTP 300

Query: 301 LVVGMGAACELAMKEMEYDDKRITALQERLLNGINAKLEGVVVNGSMERRYPGNLNLSFA 360
           LVVGMGAACELAMKEMEYDDKRITALQERLLNGINAKLEGVVVNGSME RYPGNLNLSFA
Sbjct: 301 LVVGMGAACELAMKEMEYDDKRITALQERLLNGINAKLEGVVVNGSMENRYPGNLNLSFA 360

Query: 361 YVEGESLLMGLKDVAVSSGSACTSASLEPSYVLRALGVDEDMAHTSIRFGIGRFTTEAEI 420
           YVEGESLLMGLKDVAVSSGSACTSASLEPSYVLRALGVDEDMAHTSIRFGIGRFTTEAEI
Sbjct: 361 YVEGESLLMGLKDVAVSSGSACTSASLEPSYVLRALGVDEDMAHTSIRFGIGRFTTEAEI 420

Query: 421 DRAVELTVQQVEKLREMSPLYEMVKEGINIKEIQWAQH 458
           DRAVELTV QVEKLREMSPLYEMVKEGINIKEIQWAQH
Sbjct: 421 DRAVELTVHQVEKLREMSPLYEMVKEGINIKEIQWAQH 458

BLAST of Bhi03G000832 vs. ExPASy TrEMBL
Match: A0A6J1BUZ1 (Cysteine desulfurase OS=Momordica charantia OX=3673 GN=LOC111005592 PE=3 SV=1)

HSP 1 Score: 861.3 bits (2224), Expect = 1.8e-246
Identity = 433/457 (94.75%), Postives = 445/457 (97.37%), Query Frame = 0

Query: 1   MASKMLASTLRRTLTNFSPAVSRFRPISTAAAVAASPSEPQEDETGISMKGVKISGRPLY 60
           MASK+LAS+LRRTLT  + A S  RP+STAAAV  SPSEP EDETGISMKGVKISGRPLY
Sbjct: 1   MASKILASSLRRTLTKSTQAASLLRPLSTAAAVVTSPSEPHEDETGISMKGVKISGRPLY 60

Query: 61  LDMQATSPVDPRVLDAMLPYYLSRYGNPHSRTHLYGWESDHAVETARSQVAALIGASPKE 120
           LDMQATSPVDPRVLDAMLPYYLSRYGNPHSRTHLYGWESDHAVETARSQVAALIGASPKE
Sbjct: 61  LDMQATSPVDPRVLDAMLPYYLSRYGNPHSRTHLYGWESDHAVETARSQVAALIGASPKE 120

Query: 121 IIFTSGATESNNISIKGVMHFYREKKRHLITTQTEHKCVLDSCRHLQQEGFDITYLPVGS 180
           I+FTSGATESNNIS+KGVMHFYREKKRHLITTQTEHKCVLDSCRHLQQEGFDITYLPVGS
Sbjct: 121 IVFTSGATESNNISVKGVMHFYREKKRHLITTQTEHKCVLDSCRHLQQEGFDITYLPVGS 180

Query: 181 DGIVDLDKLRSAIRADTGLVSVMAVNNEIGVIQPVEEIGKICKEFDVPFHTDAAQALGKI 240
           DGIVDL+KLRSAIR +TGLVSVMAVNNEIGVIQP+EEIGKICKEF VPFHTDAAQALGKI
Sbjct: 181 DGIVDLEKLRSAIRPETGLVSVMAVNNEIGVIQPMEEIGKICKEFGVPFHTDAAQALGKI 240

Query: 241 PIDVEKWNVSLMSLSGHKIYGPKGIGALYMRRRPRIRVEPQMNGGGQERGIRSGTVPTPL 300
           PIDVEKWNVSLMSLSGHK+YGPKGIGALY+RRRPRIRVEPQMNGGGQERGIRSGTVPTPL
Sbjct: 241 PIDVEKWNVSLMSLSGHKVYGPKGIGALYLRRRPRIRVEPQMNGGGQERGIRSGTVPTPL 300

Query: 301 VVGMGAACELAMKEMEYDDKRITALQERLLNGINAKLEGVVVNGSMERRYPGNLNLSFAY 360
           VVGMGAACELAMKEMEYDDKRITALQERLLNGINAKLEGVVVNGSMERRYPGNLNLSFAY
Sbjct: 301 VVGMGAACELAMKEMEYDDKRITALQERLLNGINAKLEGVVVNGSMERRYPGNLNLSFAY 360

Query: 361 VEGESLLMGLKDVAVSSGSACTSASLEPSYVLRALGVDEDMAHTSIRFGIGRFTTEAEID 420
           VEGESLLMGLKDVAVSSGSACTSASLEPSYVLRALGVDEDMAHTSIRFGIGRFTTEAEID
Sbjct: 361 VEGESLLMGLKDVAVSSGSACTSASLEPSYVLRALGVDEDMAHTSIRFGIGRFTTEAEID 420

Query: 421 RAVELTVQQVEKLREMSPLYEMVKEGINIKEIQWAQH 458
           RA+ELTV QVEKLREMSPLYEMVKEGINIKEIQWAQH
Sbjct: 421 RAIELTVHQVEKLREMSPLYEMVKEGINIKEIQWAQH 457

BLAST of Bhi03G000832 vs. ExPASy TrEMBL
Match: A0A6J1KNP3 (Cysteine desulfurase OS=Cucurbita maxima OX=3661 GN=LOC111496923 PE=3 SV=1)

HSP 1 Score: 860.1 bits (2221), Expect = 4.1e-246
Identity = 433/457 (94.75%), Postives = 447/457 (97.81%), Query Frame = 0

Query: 1   MASKMLASTLRRTLTNFSPAVSRFRPISTAAAVAASPSEPQEDETGISMKGVKISGRPLY 60
           MASK+LAS LRRTLT FSPAV R R +STAAAVA SPSE  EDE+GISMKGVKISGRPLY
Sbjct: 1   MASKILASGLRRTLTGFSPAVLRLRHLSTAAAVATSPSELHEDESGISMKGVKISGRPLY 60

Query: 61  LDMQATSPVDPRVLDAMLPYYLSRYGNPHSRTHLYGWESDHAVETARSQVAALIGASPKE 120
           LDMQATSPVDPRVLDAMLPYYLSRYGNPHSRTHLYGWESDHAVETARSQVAALIGASPKE
Sbjct: 61  LDMQATSPVDPRVLDAMLPYYLSRYGNPHSRTHLYGWESDHAVETARSQVAALIGASPKE 120

Query: 121 IIFTSGATESNNISIKGVMHFYREKKRHLITTQTEHKCVLDSCRHLQQEGFDITYLPVGS 180
           I+FTSGATESNNIS+KGVMHFY+EKK+H+ITTQTEHKCVLDSCRHLQQEGFDITYLPVG+
Sbjct: 121 IVFTSGATESNNISVKGVMHFYKEKKKHVITTQTEHKCVLDSCRHLQQEGFDITYLPVGT 180

Query: 181 DGIVDLDKLRSAIRADTGLVSVMAVNNEIGVIQPVEEIGKICKEFDVPFHTDAAQALGKI 240
           DGIVDL+KLRSAIR DTGLVS+MAVNNEIGVIQPVEEIGKICKEF+VPFHTDAAQALGKI
Sbjct: 181 DGIVDLEKLRSAIRPDTGLVSIMAVNNEIGVIQPVEEIGKICKEFNVPFHTDAAQALGKI 240

Query: 241 PIDVEKWNVSLMSLSGHKIYGPKGIGALYMRRRPRIRVEPQMNGGGQERGIRSGTVPTPL 300
           PIDVEKWNVSLMSLSGHKIYGPKGIGALY+RRRPRIRVEPQMNGGGQERGIRSGTVPTPL
Sbjct: 241 PIDVEKWNVSLMSLSGHKIYGPKGIGALYLRRRPRIRVEPQMNGGGQERGIRSGTVPTPL 300

Query: 301 VVGMGAACELAMKEMEYDDKRITALQERLLNGINAKLEGVVVNGSMERRYPGNLNLSFAY 360
           VVGMGAACELAMKEMEYDDKRITALQERLLNGINAKLEGVVVNGSME+RYPGNLNLSFAY
Sbjct: 301 VVGMGAACELAMKEMEYDDKRITALQERLLNGINAKLEGVVVNGSMEKRYPGNLNLSFAY 360

Query: 361 VEGESLLMGLKDVAVSSGSACTSASLEPSYVLRALGVDEDMAHTSIRFGIGRFTTEAEID 420
           VEGESLLMGLKDVAVSSGSACTSASLEPSYVLRALGVDEDMAHTSIRFGIGRFTTEAEID
Sbjct: 361 VEGESLLMGLKDVAVSSGSACTSASLEPSYVLRALGVDEDMAHTSIRFGIGRFTTEAEID 420

Query: 421 RAVELTVQQVEKLREMSPLYEMVKEGINIKEIQWAQH 458
           RAVELTV QVEKLREMSPLYEMVKEGINIKEIQWAQH
Sbjct: 421 RAVELTVHQVEKLREMSPLYEMVKEGINIKEIQWAQH 457

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
AT5G65720.16.5e-20476.37nitrogen fixation S (NIFS)-like 1 [more]
AT5G65720.21.6e-15781.85nitrogen fixation S (NIFS)-like 1 [more]
AT1G08490.13.5e-2429.78chloroplastic NIFS-like cysteine desulfurase [more]
AT1G27980.14.6e-0825.88dihydrosphingosine phosphate lyase [more]
Match NameE-valueIdentityDescription
O495439.1e-20376.37Cysteine desulfurase, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=NIFS1 PE=... [more]
Q5RDE71.0e-16971.32Cysteine desulfurase, mitochondrial OS=Pongo abelii OX=9601 GN=NFS1 PE=2 SV=1[more]
Q9Y6972.3e-16971.32Cysteine desulfurase, mitochondrial OS=Homo sapiens OX=9606 GN=NFS1 PE=1 SV=3[more]
Q9Z1J39.6e-16870.57Cysteine desulfurase, mitochondrial OS=Mus musculus OX=10090 GN=Nfs1 PE=1 SV=3[more]
Q9VKD33.6e-16764.47Cysteine desulfurase, mitochondrial OS=Drosophila melanogaster OX=7227 GN=Nfs1 P... [more]
Match NameE-valueIdentityDescription
XP_038882447.19.6e-258100.00cysteine desulfurase, mitochondrial [Benincasa hispida][more]
XP_008440458.15.1e-25196.94PREDICTED: cysteine desulfurase, mitochondrial [Cucumis melo] >KAA0036384.1 cyst... [more]
XP_011657914.12.4e-24896.29cysteine desulfurase, mitochondrial [Cucumis sativus][more]
XP_023518255.11.7e-24694.97cysteine desulfurase, mitochondrial [Cucurbita pepo subsp. pepo][more]
XP_022132842.13.8e-24694.75cysteine desulfurase, mitochondrial [Momordica charantia][more]
Match NameE-valueIdentityDescription
A0A5D3CMX02.5e-25196.94Cysteine desulfurase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold255... [more]
A0A1S3B0Q92.5e-25196.94Cysteine desulfurase OS=Cucumis melo OX=3656 GN=LOC103484889 PE=3 SV=1[more]
A0A0A0KIM01.1e-24896.29Cysteine desulfurase OS=Cucumis sativus OX=3659 GN=Csa_6G497010 PE=3 SV=1[more]
A0A6J1BUZ11.8e-24694.75Cysteine desulfurase OS=Momordica charantia OX=3673 GN=LOC111005592 PE=3 SV=1[more]
A0A6J1KNP34.1e-24694.75Cysteine desulfurase OS=Cucurbita maxima OX=3661 GN=LOC111496923 PE=3 SV=1[more]
InterPro
Analysis Name: InterPro Annotations of Wax gourd (B227) v1
Date Performed: 2021-10-22
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR015421Pyridoxal phosphate-dependent transferase, major domainGENE3D3.40.640.10coord: 70..315
e-value: 1.9E-151
score: 506.2
IPR015422Pyridoxal phosphate-dependent transferase, small domainGENE3D3.90.1150.10Aspartate Aminotransferase, domain 1coord: 60..421
e-value: 1.9E-151
score: 506.2
IPR000192Aminotransferase class V domainPFAMPF00266Aminotran_5coord: 60..421
e-value: 9.4E-87
score: 291.4
IPR010240Cysteine desulfurase IscSTIGRFAMTIGR02006TIGR02006coord: 57..457
e-value: 1.4E-198
score: 657.8
IPR010240Cysteine desulfurase IscSPANTHERPTHR11601:SF34CYSTEINE DESULFURASE, MITOCHONDRIALcoord: 49..443
IPR010240Cysteine desulfurase IscSHAMAPMF_00331Cys_desulf_IscScoord: 55..448
score: 52.594578
IPR016454Cysteine desulfurasePIRSFPIRSF005572NifScoord: 57..437
e-value: 4.2E-134
score: 445.1
NoneNo IPR availablePANTHERPTHR11601CYSTEINE DESULFURYLASE FAMILY MEMBERcoord: 49..443
IPR015424Pyridoxal phosphate-dependent transferaseSUPERFAMILY53383PLP-dependent transferasescoord: 58..444

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Bhi03M000832Bhi03M000832mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0044571 [2Fe-2S] cluster assembly
cellular_component GO:0005829 cytosol
cellular_component GO:0005739 mitochondrion
molecular_function GO:0031071 cysteine desulfurase activity
molecular_function GO:0051536 iron-sulfur cluster binding
molecular_function GO:0046872 metal ion binding
molecular_function GO:0030170 pyridoxal phosphate binding
molecular_function GO:0003824 catalytic activity