Bhi02G001674 (gene) Wax gourd (B227) v1

Overview
NameBhi02G001674
Typegene
OrganismBenincasa hispida (Wax gourd (B227) v1)
Descriptionwee1-like protein kinase
Locationchr2: 54709115 .. 54709857 (-)
RNA-Seq ExpressionBhi02G001674
SyntenyBhi02G001674
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGAGCTCTATGATTGTAGTTTATCAATTGGCAAGTATTCTCACCCATTCTCTAATGTAGATGCACTGAAAGCCTTGTATCAGGTGAGATTTAGTTCCATTTTGAGCTGTGAAACATTACATTCTCTGCATATGACATTTCATTCCAAAATTGTTTCTTTTTTTTAACACACGATGACATTAATGAAAGCTTAATTTTAATTTGTTACTTGAAAGAGTAAATTTTTTATTTCTGCAGTTCATTCAATATGTTAATTTGAACAGGAAATGCCTCTTCGGAAAAATAATGATCCAGAATGTTATAAGTTATGTACTATTTTTTTTTAATCTTTATACATTCCAATTCTTATGTAGCCTTAAAATGTTTAATATGTAAGCTTTATCTATTCCAAATTTTAAGTTCTAAAACTGGTAACTCTTGAAATTAATGGCTTCTCTACTGACAATGAAACTACAAATAGATTGCAAAGGCATTGCTCTTTGTGCATGAGAAAGGAGTAGCTCATTTAGACGTGAAACCTGACAATATTTATGTCAAAGATGGTGTCTTTAAACTTGGTGATTTTGGGTGTGTTACCTTTCTTGATAAGAACCTACCGATTGAGGAGGGTGATGCACGCTATATGCCTCAAGAAATTCTGAATGAAAGATGTGATTATCTTGACAAAGTTGATATTTTCTCCTTGGGAGCTGCTATTTATGAGATTTCCGAGGATCCACACTACCAGAAACGCATTTCATGA

mRNA sequence

ATGGAGCTCTATGATTGTAGTTTATCAATTGGCAAGTATTCTCACCCATTCTCTAATGTAGATGCACTGAAAGCCTTGTATCAGATTGCAAAGGCATTGCTCTTTGTGCATGAGAAAGGAGTAGCTCATTTAGACGTGAAACCTGACAATATTTATGTCAAAGATGGTGTCTTTAAACTTGGTGATTTTGGGTGTGTTACCTTTCTTGATAAGAACCTACCGATTGAGGAGGGTGATGCACGCTATATGCCTCAAGAAATTCTGAATGAAAGATGTGATTATCTTGACAAAGTTGATATTTTCTCCTTGGGAGCTGCTATTTATGAGATTTCCGAGGATCCACACTACCAGAAACGCATTTCATGA

Coding sequence (CDS)

ATGGAGCTCTATGATTGTAGTTTATCAATTGGCAAGTATTCTCACCCATTCTCTAATGTAGATGCACTGAAAGCCTTGTATCAGATTGCAAAGGCATTGCTCTTTGTGCATGAGAAAGGAGTAGCTCATTTAGACGTGAAACCTGACAATATTTATGTCAAAGATGGTGTCTTTAAACTTGGTGATTTTGGGTGTGTTACCTTTCTTGATAAGAACCTACCGATTGAGGAGGGTGATGCACGCTATATGCCTCAAGAAATTCTGAATGAAAGATGTGATTATCTTGACAAAGTTGATATTTTCTCCTTGGGAGCTGCTATTTATGAGATTTCCGAGGATCCACACTACCAGAAACGCATTTCATGA

Protein sequence

MELYDCSLSIGKYSHPFSNVDALKALYQIAKALLFVHEKGVAHLDVKPDNIYVKDGVFKLGDFGCVTFLDKNLPIEEGDARYMPQEILNERCDYLDKVDIFSLGAAIYEISEDPHYQKRIS
Homology
BLAST of Bhi02G001674 vs. TAIR 10
Match: AT1G02970.1 (WEE1 kinase homolog )

HSP 1 Score: 153.3 bits (386), Expect = 1.3e-37
Identity = 76/112 (67.86%), Postives = 89/112 (79.46%), Query Frame = 0

Query: 1   MELYDCSLSI--GKYSHPFSNVDALKALYQIAKALLFVHEKGVAHLDVKPDNIYVKDGVF 60
           +EL D SLS    K S   S  + L  ++QIAKAL FVHEKG+AHLDVKPDNIY+K+GV 
Sbjct: 326 LELCDHSLSALPKKSSLKVSEREILVIMHQIAKALHFVHEKGIAHLDVKPDNIYIKNGVC 385

Query: 61  KLGDFGCVTFLDKNLPIEEGDARYMPQEILNERCDYLDKVDIFSLGAAIYEI 111
           KLGDFGC T LDK+LP+EEGDARYMPQEILNE  ++LDKVDIFSLG  +YE+
Sbjct: 386 KLGDFGCATRLDKSLPVEEGDARYMPQEILNEDYEHLDKVDIFSLGVTVYEL 437

BLAST of Bhi02G001674 vs. TAIR 10
Match: AT5G45430.1 (Protein kinase superfamily protein )

HSP 1 Score: 62.4 bits (150), Expect = 2.9e-10
Identity = 33/113 (29.20%), Postives = 57/113 (50.44%), Query Frame = 0

Query: 2   ELYDCSLSIGKYSHP--FSNVDALKALYQIAKALLFVHEKGVAHLDVKPDNIYVKDGVFK 61
           E  +C+L       P  F+  D     +Q+ + L ++H++G  H D+KP+N+ V   V K
Sbjct: 80  EYMECNLYQLMKDRPKHFAESDIRNWCFQVFQGLSYMHQRGYFHRDLKPENLLVSKDVIK 139

Query: 62  LGDFGCVTFLDKNLPIEE--GDARYMPQEILNERCDYLDKVDIFSLGAAIYEI 111
           + D G    +D + P  E      Y   E+L +   Y  KVD++++GA + E+
Sbjct: 140 IADLGLAREIDSSPPYTEYVSTRWYRAPEVLLQSYVYTSKVDMWAMGAIMAEL 192

BLAST of Bhi02G001674 vs. TAIR 10
Match: AT5G45430.2 (Protein kinase superfamily protein )

HSP 1 Score: 62.4 bits (150), Expect = 2.9e-10
Identity = 33/113 (29.20%), Postives = 57/113 (50.44%), Query Frame = 0

Query: 2   ELYDCSLSIGKYSHP--FSNVDALKALYQIAKALLFVHEKGVAHLDVKPDNIYVKDGVFK 61
           E  +C+L       P  F+  D     +Q+ + L ++H++G  H D+KP+N+ V   V K
Sbjct: 80  EYMECNLYQLMKDRPKHFAESDIRNWCFQVFQGLSYMHQRGYFHRDLKPENLLVSKDVIK 139

Query: 62  LGDFGCVTFLDKNLPIEE--GDARYMPQEILNERCDYLDKVDIFSLGAAIYEI 111
           + D G    +D + P  E      Y   E+L +   Y  KVD++++GA + E+
Sbjct: 140 IADLGLAREIDSSPPYTEYVSTRWYRAPEVLLQSYVYTSKVDMWAMGAIMAEL 192

BLAST of Bhi02G001674 vs. TAIR 10
Match: AT4G19110.1 (Protein kinase superfamily protein )

HSP 1 Score: 62.0 bits (149), Expect = 3.8e-10
Identity = 28/96 (29.17%), Postives = 52/96 (54.17%), Query Frame = 0

Query: 17  FSNVDALKALYQIAKALLFVHEKGVAHLDVKPDNIYVKDGVFKLGDFGCVTFLDKNLPIE 76
           F+  D     +Q+ + L ++H++G  H D+KP+N+ V   + K+ DFG    ++ + P  
Sbjct: 97  FAEADIKNWCFQVFQGLSYMHQRGYFHRDLKPENLLVSKDIIKIADFGLAREVNSSPPFT 156

Query: 77  E--GDARYMPQEILNERCDYLDKVDIFSLGAAIYEI 111
           E      Y   E+L +   Y  KVD++++GA + E+
Sbjct: 157 EYVSTRWYRAPEVLLQSYVYTSKVDMWAMGAIMAEL 192

BLAST of Bhi02G001674 vs. TAIR 10
Match: AT4G19110.2 (Protein kinase superfamily protein )

HSP 1 Score: 62.0 bits (149), Expect = 3.8e-10
Identity = 28/96 (29.17%), Postives = 52/96 (54.17%), Query Frame = 0

Query: 17  FSNVDALKALYQIAKALLFVHEKGVAHLDVKPDNIYVKDGVFKLGDFGCVTFLDKNLPIE 76
           F+  D     +Q+ + L ++H++G  H D+KP+N+ V   + K+ DFG    ++ + P  
Sbjct: 97  FAEADIKNWCFQVFQGLSYMHQRGYFHRDLKPENLLVSKDIIKIADFGLAREVNSSPPFT 156

Query: 77  E--GDARYMPQEILNERCDYLDKVDIFSLGAAIYEI 111
           E      Y   E+L +   Y  KVD++++GA + E+
Sbjct: 157 EYVSTRWYRAPEVLLQSYVYTSKVDMWAMGAIMAEL 192

BLAST of Bhi02G001674 vs. ExPASy Swiss-Prot
Match: Q8L4H0 (Wee1-like protein kinase OS=Arabidopsis thaliana OX=3702 GN=WEE1 PE=1 SV=1)

HSP 1 Score: 153.3 bits (386), Expect = 1.8e-36
Identity = 76/112 (67.86%), Postives = 89/112 (79.46%), Query Frame = 0

Query: 1   MELYDCSLSI--GKYSHPFSNVDALKALYQIAKALLFVHEKGVAHLDVKPDNIYVKDGVF 60
           +EL D SLS    K S   S  + L  ++QIAKAL FVHEKG+AHLDVKPDNIY+K+GV 
Sbjct: 326 LELCDHSLSALPKKSSLKVSEREILVIMHQIAKALHFVHEKGIAHLDVKPDNIYIKNGVC 385

Query: 61  KLGDFGCVTFLDKNLPIEEGDARYMPQEILNERCDYLDKVDIFSLGAAIYEI 111
           KLGDFGC T LDK+LP+EEGDARYMPQEILNE  ++LDKVDIFSLG  +YE+
Sbjct: 386 KLGDFGCATRLDKSLPVEEGDARYMPQEILNEDYEHLDKVDIFSLGVTVYEL 437

BLAST of Bhi02G001674 vs. ExPASy Swiss-Prot
Match: Q6Z829 (Wee1-like protein kinase OS=Oryza sativa subsp. japonica OX=39947 GN=WEE1 PE=3 SV=1)

HSP 1 Score: 151.4 bits (381), Expect = 6.7e-36
Identity = 69/110 (62.73%), Postives = 91/110 (82.73%), Query Frame = 0

Query: 1   MELYDCSLSIGKYSHPFSNVDALKALYQIAKALLFVHEKGVAHLDVKPDNIYVKDGVFKL 60
           MEL D  LS+ + + P    +AL+ LYQI K L F+HE+G+AHLDVKPDNIYV++GV+KL
Sbjct: 344 MELCDRCLSMDR-NQPLKCGEALELLYQICKGLDFIHERGIAHLDVKPDNIYVRNGVYKL 403

Query: 61  GDFGCVTFLDKNLPIEEGDARYMPQEILNERCDYLDKVDIFSLGAAIYEI 111
           GDFGC T +D++L IE+GD+RYMP E+LN++ ++LDKVDIFSLGAAIYE+
Sbjct: 404 GDFGCATLIDRSLAIEDGDSRYMPPEMLNDKYEHLDKVDIFSLGAAIYEL 452

BLAST of Bhi02G001674 vs. ExPASy Swiss-Prot
Match: Q54E34 (Probable protein kinase DDB_G0291842 OS=Dictyostelium discoideum OX=44689 GN=DDB_G0291842 PE=3 SV=1)

HSP 1 Score: 73.2 bits (178), Expect = 2.3e-12
Identity = 41/88 (46.59%), Postives = 54/88 (61.36%), Query Frame = 0

Query: 26  LYQIAKALLFVHEKGVAHLDVKPDN-IYVKDGVFKLGDFG-CVTFLDKNLPIEEGDARYM 85
           L  IA  L  VHEKG+ HLD+KP+N ++  DGV K+GDFG C T    +    EGD  YM
Sbjct: 188 LCDIANGLSHVHEKGIMHLDIKPENLLFSNDGVLKIGDFGVCSTTTGGD--DSEGDQIYM 247

Query: 86  PQEILNERCDYLDKVDIFSLGAAIYEIS 112
             E+LN+   +    DIFSLG  +YE++
Sbjct: 248 APELLND--IHTPSADIFSLGITLYEMA 271

BLAST of Bhi02G001674 vs. ExPASy Swiss-Prot
Match: E1BTE1 (Wee1-like protein kinase 2 OS=Gallus gallus OX=9031 GN=WEE2 PE=3 SV=1)

HSP 1 Score: 71.2 bits (173), Expect = 8.8e-12
Identity = 42/127 (33.07%), Postives = 60/127 (47.24%), Query Frame = 0

Query: 3   LYDCSLSIGKYSHPFSNVDALKALYQIAKALLFVHEKGVAHLDVKPDNIYV--------- 62
           L D  L   K    F      + L Q++  L ++H  G+ HLD+KP NI++         
Sbjct: 302 LQDVLLENAKRGQYFPEAKLKEILLQVSMGLKYIHNSGLVHLDIKPSNIFICHKLVVEGQ 361

Query: 63  ------------KDGV-FKLGDFGCVTFLDKNLPIEEGDARYMPQEILNERCDYLDKVDI 108
                         GV +K+GD G VT +  N  +EEGD R++  EIL E+  +L K DI
Sbjct: 362 AGQEESDSDDEFSSGVMYKIGDLGHVTSI-ANPQVEEGDRRFLANEILQEQYFHLPKADI 421

BLAST of Bhi02G001674 vs. ExPASy Swiss-Prot
Match: Q9WVS4 (MAPK/MAK/MRK overlapping kinase OS=Mus musculus OX=10090 GN=Mok PE=1 SV=1)

HSP 1 Score: 70.9 bits (172), Expect = 1.1e-11
Identity = 38/99 (38.38%), Postives = 50/99 (50.51%), Query Frame = 0

Query: 15  HPFSNVDALKALYQIAKALLFVHEKGVAHLDVKPDNIYVKDGVFKLGDFGCVTFLDKNLP 74
           HP S    +  +YQ+ K+L  +H  G+ H DVKP+NI VK  V KLGDFG    +    P
Sbjct: 98  HPLSEKKIMLYMYQLCKSLDHMHRNGIFHRDVKPENILVKQDVLKLGDFGSCRSVYSKQP 157

Query: 75  IEE--GDARYMPQEILNERCDYLDKVDIFSLGAAIYEIS 112
             E      Y   E L     Y  K+D++S G   YEI+
Sbjct: 158 YTEYISTRWYRAPECLLTDGFYTYKMDLWSAGCVFYEIA 196

BLAST of Bhi02G001674 vs. NCBI nr
Match: XP_038876752.1 (wee1-like protein kinase [Benincasa hispida])

HSP 1 Score: 213.4 bits (542), Expect = 1.1e-51
Identity = 102/110 (92.73%), Postives = 105/110 (95.45%), Query Frame = 0

Query: 1   MELYDCSLSIGKYSHPFSNVDALKALYQIAKALLFVHEKGVAHLDVKPDNIYVKDGVFKL 60
           MEL DCSLSIG+YSHPF  VDAL+ALYQIAKALLFVHEKGVAHLDVKPDNIYVKDGVFKL
Sbjct: 320 MELCDCSLSIGRYSHPFFEVDALRALYQIAKALLFVHEKGVAHLDVKPDNIYVKDGVFKL 379

Query: 61  GDFGCVTFLDKNLPIEEGDARYMPQEILNERCDYLDKVDIFSLGAAIYEI 111
           GDFGCVT LDK+LPIEEGDARYMPQEILNER DYLDKVDIFSLGAAIYEI
Sbjct: 380 GDFGCVTLLDKSLPIEEGDARYMPQEILNERYDYLDKVDIFSLGAAIYEI 429

BLAST of Bhi02G001674 vs. NCBI nr
Match: XP_004136046.1 (wee1-like protein kinase isoform X2 [Cucumis sativus] >KGN44910.1 hypothetical protein Csa_015648 [Cucumis sativus])

HSP 1 Score: 212.6 bits (540), Expect = 1.9e-51
Identity = 99/110 (90.00%), Postives = 106/110 (96.36%), Query Frame = 0

Query: 1   MELYDCSLSIGKYSHPFSNVDALKALYQIAKALLFVHEKGVAHLDVKPDNIYVKDGVFKL 60
           MEL DCSLS+G+YSHPFS VDAL+ALYQIAKALLFVHEKG+AHLDVKPDNIY+KDGV+KL
Sbjct: 315 MELCDCSLSMGRYSHPFSEVDALRALYQIAKALLFVHEKGIAHLDVKPDNIYIKDGVYKL 374

Query: 61  GDFGCVTFLDKNLPIEEGDARYMPQEILNERCDYLDKVDIFSLGAAIYEI 111
           GDFGCVT LDK+LPIEEGDARYMPQEILNER DYLDKVDIFSLGAAIYEI
Sbjct: 375 GDFGCVTLLDKSLPIEEGDARYMPQEILNERYDYLDKVDIFSLGAAIYEI 424

BLAST of Bhi02G001674 vs. NCBI nr
Match: XP_031744240.1 (wee1-like protein kinase isoform X1 [Cucumis sativus])

HSP 1 Score: 212.6 bits (540), Expect = 1.9e-51
Identity = 99/110 (90.00%), Postives = 106/110 (96.36%), Query Frame = 0

Query: 1   MELYDCSLSIGKYSHPFSNVDALKALYQIAKALLFVHEKGVAHLDVKPDNIYVKDGVFKL 60
           MEL DCSLS+G+YSHPFS VDAL+ALYQIAKALLFVHEKG+AHLDVKPDNIY+KDGV+KL
Sbjct: 316 MELCDCSLSMGRYSHPFSEVDALRALYQIAKALLFVHEKGIAHLDVKPDNIYIKDGVYKL 375

Query: 61  GDFGCVTFLDKNLPIEEGDARYMPQEILNERCDYLDKVDIFSLGAAIYEI 111
           GDFGCVT LDK+LPIEEGDARYMPQEILNER DYLDKVDIFSLGAAIYEI
Sbjct: 376 GDFGCVTLLDKSLPIEEGDARYMPQEILNERYDYLDKVDIFSLGAAIYEI 425

BLAST of Bhi02G001674 vs. NCBI nr
Match: TYK18102.1 (wee1-like protein kinase [Cucumis melo var. makuwa])

HSP 1 Score: 211.5 bits (537), Expect = 4.2e-51
Identity = 99/110 (90.00%), Postives = 105/110 (95.45%), Query Frame = 0

Query: 1   MELYDCSLSIGKYSHPFSNVDALKALYQIAKALLFVHEKGVAHLDVKPDNIYVKDGVFKL 60
           MEL DCSLS+G YSHPFS VDAL+ALYQIAKALLFVHEKG+AHLDVKPDNIY+KDGV+KL
Sbjct: 457 MELCDCSLSMGTYSHPFSEVDALRALYQIAKALLFVHEKGIAHLDVKPDNIYIKDGVYKL 516

Query: 61  GDFGCVTFLDKNLPIEEGDARYMPQEILNERCDYLDKVDIFSLGAAIYEI 111
           GDFGCVT LDK+LPIEEGDARYMPQEILNER DYLDKVDIFSLGAAIYEI
Sbjct: 517 GDFGCVTLLDKSLPIEEGDARYMPQEILNERYDYLDKVDIFSLGAAIYEI 566

BLAST of Bhi02G001674 vs. NCBI nr
Match: KAA0068026.1 (wee1-like protein kinase [Cucumis melo var. makuwa])

HSP 1 Score: 211.5 bits (537), Expect = 4.2e-51
Identity = 99/110 (90.00%), Postives = 105/110 (95.45%), Query Frame = 0

Query: 1   MELYDCSLSIGKYSHPFSNVDALKALYQIAKALLFVHEKGVAHLDVKPDNIYVKDGVFKL 60
           MEL DCSLS+G YSHPFS VDAL+ALYQIAKALLFVHEKG+AHLDVKPDNIY+KDGV+KL
Sbjct: 304 MELCDCSLSMGTYSHPFSEVDALRALYQIAKALLFVHEKGIAHLDVKPDNIYIKDGVYKL 363

Query: 61  GDFGCVTFLDKNLPIEEGDARYMPQEILNERCDYLDKVDIFSLGAAIYEI 111
           GDFGCVT LDK+LPIEEGDARYMPQEILNER DYLDKVDIFSLGAAIYEI
Sbjct: 364 GDFGCVTLLDKSLPIEEGDARYMPQEILNERYDYLDKVDIFSLGAAIYEI 413

BLAST of Bhi02G001674 vs. ExPASy TrEMBL
Match: A0A0A0K5B3 (Protein kinase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_7G395770 PE=3 SV=1)

HSP 1 Score: 212.6 bits (540), Expect = 9.0e-52
Identity = 99/110 (90.00%), Postives = 106/110 (96.36%), Query Frame = 0

Query: 1   MELYDCSLSIGKYSHPFSNVDALKALYQIAKALLFVHEKGVAHLDVKPDNIYVKDGVFKL 60
           MEL DCSLS+G+YSHPFS VDAL+ALYQIAKALLFVHEKG+AHLDVKPDNIY+KDGV+KL
Sbjct: 315 MELCDCSLSMGRYSHPFSEVDALRALYQIAKALLFVHEKGIAHLDVKPDNIYIKDGVYKL 374

Query: 61  GDFGCVTFLDKNLPIEEGDARYMPQEILNERCDYLDKVDIFSLGAAIYEI 111
           GDFGCVT LDK+LPIEEGDARYMPQEILNER DYLDKVDIFSLGAAIYEI
Sbjct: 375 GDFGCVTLLDKSLPIEEGDARYMPQEILNERYDYLDKVDIFSLGAAIYEI 424

BLAST of Bhi02G001674 vs. ExPASy TrEMBL
Match: A0A5D3D2H5 (Wee1-like protein kinase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold411G00240 PE=4 SV=1)

HSP 1 Score: 211.5 bits (537), Expect = 2.0e-51
Identity = 99/110 (90.00%), Postives = 105/110 (95.45%), Query Frame = 0

Query: 1   MELYDCSLSIGKYSHPFSNVDALKALYQIAKALLFVHEKGVAHLDVKPDNIYVKDGVFKL 60
           MEL DCSLS+G YSHPFS VDAL+ALYQIAKALLFVHEKG+AHLDVKPDNIY+KDGV+KL
Sbjct: 457 MELCDCSLSMGTYSHPFSEVDALRALYQIAKALLFVHEKGIAHLDVKPDNIYIKDGVYKL 516

Query: 61  GDFGCVTFLDKNLPIEEGDARYMPQEILNERCDYLDKVDIFSLGAAIYEI 111
           GDFGCVT LDK+LPIEEGDARYMPQEILNER DYLDKVDIFSLGAAIYEI
Sbjct: 517 GDFGCVTLLDKSLPIEEGDARYMPQEILNERYDYLDKVDIFSLGAAIYEI 566

BLAST of Bhi02G001674 vs. ExPASy TrEMBL
Match: A0A5A7VN66 (Wee1-like protein kinase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold138G002220 PE=3 SV=1)

HSP 1 Score: 211.5 bits (537), Expect = 2.0e-51
Identity = 99/110 (90.00%), Postives = 105/110 (95.45%), Query Frame = 0

Query: 1   MELYDCSLSIGKYSHPFSNVDALKALYQIAKALLFVHEKGVAHLDVKPDNIYVKDGVFKL 60
           MEL DCSLS+G YSHPFS VDAL+ALYQIAKALLFVHEKG+AHLDVKPDNIY+KDGV+KL
Sbjct: 304 MELCDCSLSMGTYSHPFSEVDALRALYQIAKALLFVHEKGIAHLDVKPDNIYIKDGVYKL 363

Query: 61  GDFGCVTFLDKNLPIEEGDARYMPQEILNERCDYLDKVDIFSLGAAIYEI 111
           GDFGCVT LDK+LPIEEGDARYMPQEILNER DYLDKVDIFSLGAAIYEI
Sbjct: 364 GDFGCVTLLDKSLPIEEGDARYMPQEILNERYDYLDKVDIFSLGAAIYEI 413

BLAST of Bhi02G001674 vs. ExPASy TrEMBL
Match: A0A1S3BRY6 (wee1-like protein kinase OS=Cucumis melo OX=3656 GN=LOC103492846 PE=3 SV=1)

HSP 1 Score: 211.5 bits (537), Expect = 2.0e-51
Identity = 99/110 (90.00%), Postives = 105/110 (95.45%), Query Frame = 0

Query: 1   MELYDCSLSIGKYSHPFSNVDALKALYQIAKALLFVHEKGVAHLDVKPDNIYVKDGVFKL 60
           MEL DCSLS+G YSHPFS VDAL+ALYQIAKALLFVHEKG+AHLDVKPDNIY+KDGV+KL
Sbjct: 315 MELCDCSLSMGTYSHPFSEVDALRALYQIAKALLFVHEKGIAHLDVKPDNIYIKDGVYKL 374

Query: 61  GDFGCVTFLDKNLPIEEGDARYMPQEILNERCDYLDKVDIFSLGAAIYEI 111
           GDFGCVT LDK+LPIEEGDARYMPQEILNER DYLDKVDIFSLGAAIYEI
Sbjct: 375 GDFGCVTLLDKSLPIEEGDARYMPQEILNERYDYLDKVDIFSLGAAIYEI 424

BLAST of Bhi02G001674 vs. ExPASy TrEMBL
Match: A0A6J1KZT5 (wee1-like protein kinase OS=Cucurbita maxima OX=3661 GN=LOC111497804 PE=3 SV=1)

HSP 1 Score: 207.2 bits (526), Expect = 3.8e-50
Identity = 97/110 (88.18%), Postives = 103/110 (93.64%), Query Frame = 0

Query: 1   MELYDCSLSIGKYSHPFSNVDALKALYQIAKALLFVHEKGVAHLDVKPDNIYVKDGVFKL 60
           MEL DCSLS G+YSHPFS  DALKAL+QIAKALLF+HEKGVAHLDVKPDNIYVKDGV+KL
Sbjct: 315 MELCDCSLSTGRYSHPFSEADALKALHQIAKALLFMHEKGVAHLDVKPDNIYVKDGVYKL 374

Query: 61  GDFGCVTFLDKNLPIEEGDARYMPQEILNERCDYLDKVDIFSLGAAIYEI 111
           GDFGC T LDK+LPIEEGDARYMPQEILNER DYLDKVDIFSLGAAIYE+
Sbjct: 375 GDFGCATLLDKSLPIEEGDARYMPQEILNERYDYLDKVDIFSLGAAIYEV 424

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
AT1G02970.11.3e-3767.86WEE1 kinase homolog [more]
AT5G45430.12.9e-1029.20Protein kinase superfamily protein [more]
AT5G45430.22.9e-1029.20Protein kinase superfamily protein [more]
AT4G19110.13.8e-1029.17Protein kinase superfamily protein [more]
AT4G19110.23.8e-1029.17Protein kinase superfamily protein [more]
Match NameE-valueIdentityDescription
Q8L4H01.8e-3667.86Wee1-like protein kinase OS=Arabidopsis thaliana OX=3702 GN=WEE1 PE=1 SV=1[more]
Q6Z8296.7e-3662.73Wee1-like protein kinase OS=Oryza sativa subsp. japonica OX=39947 GN=WEE1 PE=3 S... [more]
Q54E342.3e-1246.59Probable protein kinase DDB_G0291842 OS=Dictyostelium discoideum OX=44689 GN=DDB... [more]
E1BTE18.8e-1233.07Wee1-like protein kinase 2 OS=Gallus gallus OX=9031 GN=WEE2 PE=3 SV=1[more]
Q9WVS41.1e-1138.38MAPK/MAK/MRK overlapping kinase OS=Mus musculus OX=10090 GN=Mok PE=1 SV=1[more]
Match NameE-valueIdentityDescription
XP_038876752.11.1e-5192.73wee1-like protein kinase [Benincasa hispida][more]
XP_004136046.11.9e-5190.00wee1-like protein kinase isoform X2 [Cucumis sativus] >KGN44910.1 hypothetical p... [more]
XP_031744240.11.9e-5190.00wee1-like protein kinase isoform X1 [Cucumis sativus][more]
TYK18102.14.2e-5190.00wee1-like protein kinase [Cucumis melo var. makuwa][more]
KAA0068026.14.2e-5190.00wee1-like protein kinase [Cucumis melo var. makuwa][more]
Match NameE-valueIdentityDescription
A0A0A0K5B39.0e-5290.00Protein kinase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_7G395... [more]
A0A5D3D2H52.0e-5190.00Wee1-like protein kinase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffol... [more]
A0A5A7VN662.0e-5190.00Wee1-like protein kinase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffol... [more]
A0A1S3BRY62.0e-5190.00wee1-like protein kinase OS=Cucumis melo OX=3656 GN=LOC103492846 PE=3 SV=1[more]
A0A6J1KZT53.8e-5088.18wee1-like protein kinase OS=Cucurbita maxima OX=3661 GN=LOC111497804 PE=3 SV=1[more]
InterPro
Analysis Name: InterPro Annotations of Wax gourd (B227) v1
Date Performed: 2021-10-22
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000719Protein kinase domainPFAMPF00069Pkinasecoord: 14..111
e-value: 6.5E-22
score: 78.2
IPR000719Protein kinase domainPROSITEPS50011PROTEIN_KINASE_DOMcoord: 1..121
score: 20.188311
NoneNo IPR availableGENE3D1.10.510.10Transferase(Phosphotransferase) domain 1coord: 2..119
e-value: 1.1E-26
score: 95.3
NoneNo IPR availablePANTHERPTHR11042EUKARYOTIC TRANSLATION INITIATION FACTOR 2-ALPHA KINASE EIF2-ALPHA KINASE -RELATEDcoord: 1..110
NoneNo IPR availablePANTHERPTHR11042:SF144WEE1-LIKE PROTEIN KINASEcoord: 1..110
IPR008271Serine/threonine-protein kinase, active sitePROSITEPS00108PROTEIN_KINASE_STcoord: 41..53
IPR011009Protein kinase-like domain superfamilySUPERFAMILY56112Protein kinase-like (PK-like)coord: 1..110

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Bhi02M001674Bhi02M001674mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0000165 MAPK cascade
biological_process GO:1902750 negative regulation of cell cycle G2/M phase transition
biological_process GO:0018108 peptidyl-tyrosine phosphorylation
biological_process GO:0006468 protein phosphorylation
cellular_component GO:0005634 nucleus
molecular_function GO:0005524 ATP binding
molecular_function GO:0004708 MAP kinase kinase activity
molecular_function GO:0004674 protein serine/threonine kinase activity
molecular_function GO:0004713 protein tyrosine kinase activity
molecular_function GO:0004672 protein kinase activity