Bhi02G000399 (gene) Wax gourd (B227) v1

Overview


Name	Bhi02G000399
Type	gene
Organism	Benincasa hispida (Wax gourd (B227) v1)
Description	EF hand calcium-binding family protein
Location	chr2: 10125430 .. 10126907 (-)
RNA-Seq Expression	Bhi02G000399
Synteny	Bhi02G000399

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSpolypeptidethree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

CCCATTTTCATTTTTGACCCAATTAAACACGTTCTTCTTTTTTAATCCCTTTTTTCTTTTCTCTTTTGAATTTTATACCATTCCGAGGCTTTCCTTCGCCGCAGTTTCCAAAAATATCCCGAAGCCTATAAATGGAAACCCCAAACCCTGAATTGACCAAAAATCCAAATTCAGAGAAAAATCCACAAAACTCCTTTCTAAAAATTCTCCAATCCCGAATTGAAATGGAGATCAACTGTGCGGCACAGTACGATCGCGTATTGAGTTACTTCGACGAAGATGGAGACGGCAAGATTTCGCCGTCGGAGCTGAGGAATCGGCTGGGATTAATGGGCGGAGAGCTGCTGCAGGCGGAGGCTGAGGCGGCGGTGGAATCGCTGGACTCGGACGGCGATGGATTGCTGTGCGCCGGCGATTTCGAACGGCTACTGGAGGTTGGGGAGGAAGAGAAATTGAAGGATTTGAAAGAGGCGTTTGCTCTGTACGATTGTGAAGGTTGCGGGTTCATTACTCCGAAAAATTTGAAGAAAATGCTAAGAAAATTGGGGGAAAGAAAATCCACCGAGGAGTGTAAATTGATGATTCGTCGCTTCGATTTGAACGGTGACGGTTTGATTAGCTTTGAGGAGTTTCAGATCATGATGGCTTAACTCTGTACATATCTTCATTTCTTTTTCTTTCTTACATCGACATATTCTTTTTAATTTCATTTTTTTGGATATCTGATTATTCAATTCATTTTAGTTGTTGAATTTGGGGCATTTCCCATTTGTATAAATACACAAACACATTTCTATCTGTAGACTTTTGAGATTATATGAATAAATAATGACGTGAGTTTAGCTGGGTTGAATATTCGATCTCATTTATATGGATAAATATATATGTATTTGGGTTATGAATTAAGGATGAATTGTTTATAGTCTCATTCCATGTGTTTTGAGTCTTCCAAAGAAACTATTTATGTTTATGGTTACACGTGATTAGATATTAAAATTTTTAGCATGAAAGAATTGACTCTTCTAAATGAAGAAAAAAGGGTATTTCCTTAATCCTTCCCAAGAAAGATTTTTGTTTCTTCCTATGAACTATGAAGGAGGTCTTGTCACTGTCAAATGGAAGCAGAGTTGATTAGTAATGATGATATTACGAAATAATCAACTCTCTTTGTCTCTGTCTCTTTCTTTCCTGTTTAAGAAGAAGATAGAAAAATTTTAAACATGTACTCTGTCTCTGTCTCTCTCTAGAAATAGGTCATTTCCACTTTAGTTACGTGCAAATTTAGAATTTCGATTTATGTAGGTACTTCCTTTTGAATGACATCTGTTCTCCTTTTAGTTTTTTTTGTATGTATGCATCAAGGAGTTCTCTAATCTCTAGGTGTATATGAGTTGTACTGGTTCGTGTGGATGGATTCTTTGTCGAACAGAAGTGATCACGTATGTCAATAGGGATATTTAAAAAACCTTTATCGAGGG

mRNA sequence

CCCATTTTCATTTTTGACCCAATTAAACACGTTCTTCTTTTTTAATCCCTTTTTTCTTTTCTCTTTTGAATTTTATACCATTCCGAGGCTTTCCTTCGCCGCAGTTTCCAAAAATATCCCGAAGCCTATAAATGGAAACCCCAAACCCTGAATTGACCAAAAATCCAAATTCAGAGAAAAATCCACAAAACTCCTTTCTAAAAATTCTCCAATCCCGAATTGAAATGGAGATCAACTGTGCGGCACAGTACGATCGCGTATTGAGTTACTTCGACGAAGATGGAGACGGCAAGATTTCGCCGTCGGAGCTGAGGAATCGGCTGGGATTAATGGGCGGAGAGCTGCTGCAGGCGGAGGCTGAGGCGGCGGTGGAATCGCTGGACTCGGACGGCGATGGATTGCTGTGCGCCGGCGATTTCGAACGGCTACTGGAGGTTGGGGAGGAAGAGAAATTGAAGGATTTGAAAGAGGCGTTTGCTCTGTACGATTGTGAAGGTTGCGGGTTCATTACTCCGAAAAATTTGAAGAAAATGCTAAGAAAATTGGGGGAAAGAAAATCCACCGAGGAGTGTAAATTGATGATTCGTCGCTTCGATTTGAACGGTGACGGTTTGATTAGCTTTGAGGAGTTTCAGATCATGATGGCTTAACTCTGTACATATCTTCATTTCTTTTTCTTTCTTACATCGACATATTCTTTTTAATTTCATTTTTTTGGATATCTGATTATTCAATTCATTTTAGTTGTTGAATTTGGGGCATTTCCCATTTGTATAAATACACAAACACATTTCTATCTGTAGACTTTTGAGATTATATGAATAAATAATGACGTGAGTTTAGCTGGGTTGAATATTCGATCTCATTTATATGGATAAATATATATGTATTTGGGTTATGAATTAAGGATGAATTGTTTATAGTCTCATTCCATGTGTTTTGAGTCTTCCAAAGAAACTATTTATGTTTATGGTTACACGTGATTAGATATTAAAATTTTTAGCATGAAAGAATTGACTCTTCTAAATGAAGAAAAAAGGGTATTTCCTTAATCCTTCCCAAGAAAGATTTTTGTTTCTTCCTATGAACTATGAAGGAGGTCTTGTCACTGTCAAATGGAAGCAGAGTTGATTAGTAATGATGATATTACGAAATAATCAACTCTCTTTGTCTCTGTCTCTTTCTTTCCTGTTTAAGAAGAAGATAGAAAAATTTTAAACATGTACTCTGTCTCTGTCTCTCTCTAGAAATAGGTCATTTCCACTTTAGTTACGTGCAAATTTAGAATTTCGATTTATGTAGGTACTTCCTTTTGAATGACATCTGTTCTCCTTTTAGTTTTTTTTGTATGTATGCATCAAGGAGTTCTCTAATCTCTAGGTGTATATGAGTTGTACTGGTTCGTGTGGATGGATTCTTTGTCGAACAGAAGTGATCACGTATGTCAATAGGGATATTTAAAAAACCTTTATCGAGGG

Coding sequence (CDS)

ATGGAAACCCCAAACCCTGAATTGACCAAAAATCCAAATTCAGAGAAAAATCCACAAAACTCCTTTCTAAAAATTCTCCAATCCCGAATTGAAATGGAGATCAACTGTGCGGCACAGTACGATCGCGTATTGAGTTACTTCGACGAAGATGGAGACGGCAAGATTTCGCCGTCGGAGCTGAGGAATCGGCTGGGATTAATGGGCGGAGAGCTGCTGCAGGCGGAGGCTGAGGCGGCGGTGGAATCGCTGGACTCGGACGGCGATGGATTGCTGTGCGCCGGCGATTTCGAACGGCTACTGGAGGTTGGGGAGGAAGAGAAATTGAAGGATTTGAAAGAGGCGTTTGCTCTGTACGATTGTGAAGGTTGCGGGTTCATTACTCCGAAAAATTTGAAGAAAATGCTAAGAAAATTGGGGGAAAGAAAATCCACCGAGGAGTGTAAATTGATGATTCGTCGCTTCGATTTGAACGGTGACGGTTTGATTAGCTTTGAGGAGTTTCAGATCATGATGGCTTAA

Protein sequence

METPNPELTKNPNSEKNPQNSFLKILQSRIEMEINCAAQYDRVLSYFDEDGDGKISPSELRNRLGLMGGELLQAEAEAAVESLDSDGDGLLCAGDFERLLEVGEEEKLKDLKEAFALYDCEGCGFITPKNLKKMLRKLGERKSTEECKLMIRRFDLNGDGLISFEEFQIMMA

Homology

[Close]

BLAST of Bhi02G000399 vs. TAIR 10
Match: AT1G76650.1 (calmodulin-like 38 )

HSP 1 Score: 128.6 bits (322), Expect = 4.7e-30
Identity = 66/134 (49.25%), Postives = 93/134 (69.40%), Query Frame = 0


Query: 39  QYDRVLSYFDEDGDGKISPSELRNRLGLMGGELLQAEAEAAVESLDSDGDGLLCAGDFER 98
           + + V SY D + DG+ISP EL+     +G +L   EA AAV   D+DGDG+L   +F +
Sbjct: 43  ELEAVFSYMDANRDGRISPEELQKSFMTLGEQLSDEEAVAAVRLSDTDGDGMLDFEEFSQ 102

Query: 99  LLEV-GEEEKLKDLKEAFALYDCEGCGFITPKNLKKMLRKLGERKSTEECKLMIRRFDLN 158
           L++V  EEEK  +LK AF LY  EG   ITP++LK ML+KLGE ++T++C++MI  FDLN
Sbjct: 103 LIKVDDEEEKKMELKGAFRLYIAEGEDCITPRSLKMMLKKLGESRTTDDCRVMISAFDLN 162

Query: 159 GDGLISFEEFQIMM 172
            DG++SF+EF +MM
Sbjct: 163 ADGVLSFDEFALMM 176

[Close]

BLAST of Bhi02G000399 vs. TAIR 10
Match: AT1G76650.3 (calmodulin-like 38 )

HSP 1 Score: 128.6 bits (322), Expect = 4.7e-30
Identity = 66/134 (49.25%), Postives = 93/134 (69.40%), Query Frame = 0


Query: 39  QYDRVLSYFDEDGDGKISPSELRNRLGLMGGELLQAEAEAAVESLDSDGDGLLCAGDFER 98
           + + V SY D + DG+ISP EL+     +G +L   EA AAV   D+DGDG+L   +F +
Sbjct: 43  ELEAVFSYMDANRDGRISPEELQKSFMTLGEQLSDEEAVAAVRLSDTDGDGMLDFEEFSQ 102

Query: 99  LLEV-GEEEKLKDLKEAFALYDCEGCGFITPKNLKKMLRKLGERKSTEECKLMIRRFDLN 158
           L++V  EEEK  +LK AF LY  EG   ITP++LK ML+KLGE ++T++C++MI  FDLN
Sbjct: 103 LIKVDDEEEKKMELKGAFRLYIAEGEDCITPRSLKMMLKKLGESRTTDDCRVMISAFDLN 162

Query: 159 GDGLISFEEFQIMM 172
            DG++SF+EF +MM
Sbjct: 163 ADGVLSFDEFALMM 176

[Close]

BLAST of Bhi02G000399 vs. TAIR 10
Match: AT1G76640.1 (Calcium-binding EF-hand family protein )

HSP 1 Score: 124.0 bits (310), Expect = 1.2e-28
Identity = 67/163 (41.10%), Postives = 101/163 (61.96%), Query Frame = 0


Query: 13  NSEKNPQNSFLKILQSRIEMEINCAAQYDRVLSYFDEDGDGKISPSELRNRLGLMGGELL 72
           N+++   +SF+K L+ +           + V +Y D + DG+IS  EL+     +G ++ 
Sbjct: 3   NTQRQLSSSFMKFLEEK-------NRDLEAVFAYMDANRDGRISAEELKKSFKTLGEQMS 62

Query: 73  QAEAEAAVESLDSDGDGLLCAGDFERLL----EVGEEEKLKDLKEAFALYDCEGCGFITP 132
             EAEAAV+  D DGDG+L   +F  L+    E  EEEK + + EAF +Y  +G   ITP
Sbjct: 63  DEEAEAAVKLSDIDGDGMLDINEFALLIKGNDEFTEEEKKRKIMEAFRMYIADGEDCITP 122

Query: 133 KNLKKMLRKLGERKSTEECKLMIRRFDLNGDGLISFEEFQIMM 172
            +LK ML KLGE ++T++CK+MI+ FDLN DG++SF+EF +MM
Sbjct: 123 GSLKMMLMKLGESRTTDDCKVMIQAFDLNADGVLSFDEFALMM 158

[Close]

BLAST of Bhi02G000399 vs. TAIR 10
Match: AT1G76650.2 (calmodulin-like 38 )

HSP 1 Score: 117.5 bits (293), Expect = 1.1e-26
Identity = 62/134 (46.27%), Postives = 89/134 (66.42%), Query Frame = 0


Query: 39  QYDRVLSYFDEDGDGKISPSELRNRLGLMGGELLQAEAEAAVESLDSDGDGLLCAGDFER 98
           + + V SY D + DG+ISP EL+     +G +LL           D+DGDG+L   +F +
Sbjct: 43  ELEAVFSYMDANRDGRISPEELQKSFMTLGEQLLS----------DTDGDGMLDFEEFSQ 102

Query: 99  LLEV-GEEEKLKDLKEAFALYDCEGCGFITPKNLKKMLRKLGERKSTEECKLMIRRFDLN 158
           L++V  EEEK  +LK AF LY  EG   ITP++LK ML+KLGE ++T++C++MI  FDLN
Sbjct: 103 LIKVDDEEEKKMELKGAFRLYIAEGEDCITPRSLKMMLKKLGESRTTDDCRVMISAFDLN 162

Query: 159 GDGLISFEEFQIMM 172
            DG++SF+EF +MM
Sbjct: 163 ADGVLSFDEFALMM 166

[Close]

BLAST of Bhi02G000399 vs. TAIR 10
Match: AT5G42380.1 (calmodulin like 37 )

HSP 1 Score: 114.8 bits (286), Expect = 7.0e-26
Identity = 60/132 (45.45%), Postives = 86/132 (65.15%), Query Frame = 0


Query: 43  VLSYFDEDGDGKISPSELRNRLGLMGGELLQAEAEAAVESLDSDGDGLLCAGDFERLLE- 102
           V  Y D + DGKIS  EL++ + L+GG L   E E  V++ D DGDG +   +F +L+E 
Sbjct: 53  VFDYMDANSDGKISGEELQSCVSLLGGALSSREVEEVVKTSDVDGDGFIDFEEFLKLMEG 112

Query: 103 --VGEEEKLKDLKEAFALYDCEGCGFITPKNLKKMLRKLGERKSTEECKLMIRRFDLNGD 162
               +EE+ K+LKEAF +Y  EG  FIT  +L++ L +LGE  + + CK+MIR FD N D
Sbjct: 113 EDGSDEERRKELKEAFGMYVMEGEEFITAASLRRTLSRLGESCTVDACKVMIRGFDQNDD 172

Query: 163 GLISFEEFQIMM 172
           G++SF+EF +MM
Sbjct: 173 GVLSFDEFVLMM 184

[Close]

BLAST of Bhi02G000399 vs. ExPASy Swiss-Prot
Match: Q8RYJ8 (Putative calcium-binding protein CML19 OS=Oryza sativa subsp. japonica OX=39947 GN=CML19 PE=3 SV=1)

HSP 1 Score: 132.9 bits (333), Expect = 3.5e-30
Identity = 75/143 (52.45%), Postives = 98/143 (68.53%), Query Frame = 0


Query: 34  INCAAQYDRVLSYFDEDGDGKISPSELRNRL-GLMGGELLQAEAEAAVESLDSDGDGLLC 93
           +   A++ RV S FD D DGKIS +ELR  +   +G ++   EAEA V S D+D DGLL 
Sbjct: 2   VAATAEFRRVFSAFDRDADGKISAAELRLCMKAALGEDMSAEEAEALVSSADTDDDGLLD 61

Query: 94  AGDFERL---LEVG-EEEKLKDLKEAFALYDCEGCGFITPKNLKKMLRKLGERKSTEECK 153
             +F +L   LE+G EEE+ + L EAF +Y+ EG G ITP +LK+ML KLG  +  EEC+
Sbjct: 62  EEEFTKLAVQLEMGDEEERCRGLMEAFRMYEMEGEGRITPASLKRMLSKLGSHQGIEECQ 121

Query: 154 LMIRRFDLNGDGLISFEEFQIMM 172
            MI RFDL+GDG+ISFEEF+IMM
Sbjct: 122 TMICRFDLDGDGVISFEEFKIMM 144

[Close]

BLAST of Bhi02G000399 vs. ExPASy Swiss-Prot
Match: Q9SRE6 (Calcium-binding protein CML38 OS=Arabidopsis thaliana OX=3702 GN=CML38 PE=1 SV=1)

HSP 1 Score: 128.6 bits (322), Expect = 6.6e-29
Identity = 66/134 (49.25%), Postives = 93/134 (69.40%), Query Frame = 0


Query: 39  QYDRVLSYFDEDGDGKISPSELRNRLGLMGGELLQAEAEAAVESLDSDGDGLLCAGDFER 98
           + + V SY D + DG+ISP EL+     +G +L   EA AAV   D+DGDG+L   +F +
Sbjct: 43  ELEAVFSYMDANRDGRISPEELQKSFMTLGEQLSDEEAVAAVRLSDTDGDGMLDFEEFSQ 102

Query: 99  LLEV-GEEEKLKDLKEAFALYDCEGCGFITPKNLKKMLRKLGERKSTEECKLMIRRFDLN 158
           L++V  EEEK  +LK AF LY  EG   ITP++LK ML+KLGE ++T++C++MI  FDLN
Sbjct: 103 LIKVDDEEEKKMELKGAFRLYIAEGEDCITPRSLKMMLKKLGESRTTDDCRVMISAFDLN 162

Query: 159 GDGLISFEEFQIMM 172
            DG++SF+EF +MM
Sbjct: 163 ADGVLSFDEFALMM 176

[Close]

BLAST of Bhi02G000399 vs. ExPASy Swiss-Prot
Match: Q8RYK0 (Probable calcium-binding protein CML31 OS=Oryza sativa subsp. japonica OX=39947 GN=CML31 PE=2 SV=1)

HSP 1 Score: 125.6 bits (314), Expect = 5.6e-28
Identity = 65/131 (49.62%), Postives = 92/131 (70.23%), Query Frame = 0


Query: 47  FDEDGDGKISPSELRNRL-GLMGGELLQAEAEAAVESLDSDGDGLLCAGDFERLLEVGEE 106
           FD DGDG+IS +ELR  +   +G E+   EA   V S+D+DGDGLLC  +F RL++  E 
Sbjct: 19  FDHDGDGRISAAELRLCMKTTLGEEVSDEEAGQLVASVDADGDGLLCEAEFVRLVQAAEV 78

Query: 107 EKLKD-----LKEAFALYDCEGCGFITPKNLKKMLRKLGERKSTEECKLMIRRFDLNGDG 166
           E+  +     L+EAF +Y+ EG G ITP +L++MLR+LG  +  ++C+ MI RFDLNGDG
Sbjct: 79  EEEDERRGTGLREAFGMYEMEGEGCITPTSLRRMLRRLGSDQDIDDCRAMICRFDLNGDG 138

Query: 167 LISFEEFQIMM 172
           ++SF+EF+IMM
Sbjct: 139 VLSFDEFKIMM 149

[Close]

BLAST of Bhi02G000399 vs. ExPASy Swiss-Prot
Match: Q9SRE7 (Calcium-binding protein CML39 OS=Arabidopsis thaliana OX=3702 GN=CML39 PE=2 SV=1)

HSP 1 Score: 124.0 bits (310), Expect = 1.6e-27
Identity = 67/163 (41.10%), Postives = 101/163 (61.96%), Query Frame = 0


Query: 13  NSEKNPQNSFLKILQSRIEMEINCAAQYDRVLSYFDEDGDGKISPSELRNRLGLMGGELL 72
           N+++   +SF+K L+ +           + V +Y D + DG+IS  EL+     +G ++ 
Sbjct: 3   NTQRQLSSSFMKFLEEK-------NRDLEAVFAYMDANRDGRISAEELKKSFKTLGEQMS 62

Query: 73  QAEAEAAVESLDSDGDGLLCAGDFERLL----EVGEEEKLKDLKEAFALYDCEGCGFITP 132
             EAEAAV+  D DGDG+L   +F  L+    E  EEEK + + EAF +Y  +G   ITP
Sbjct: 63  DEEAEAAVKLSDIDGDGMLDINEFALLIKGNDEFTEEEKKRKIMEAFRMYIADGEDCITP 122

Query: 133 KNLKKMLRKLGERKSTEECKLMIRRFDLNGDGLISFEEFQIMM 172
            +LK ML KLGE ++T++CK+MI+ FDLN DG++SF+EF +MM
Sbjct: 123 GSLKMMLMKLGESRTTDDCKVMIQAFDLNADGVLSFDEFALMM 158

[Close]

BLAST of Bhi02G000399 vs. ExPASy Swiss-Prot
Match: Q2QYW1 (Probable calcium-binding protein CML25/26 OS=Oryza sativa subsp. japonica OX=39947 GN=CML25 PE=3 SV=1)

HSP 1 Score: 116.3 bits (290), Expect = 3.4e-25
Identity = 68/145 (46.90%), Postives = 96/145 (66.21%), Query Frame = 0


Query: 37  AAQYDRVLSYFDEDGDGKISPSELRNRL-GLMGGELLQAEAEAAVESLDSDGDGLLCAGD 96
           A+    V + FD+DGDGK+S SELR  +   +G E+ + EA A + + D+DGDGLL   +
Sbjct: 2   ASSASSVFAAFDKDGDGKVSASELRGCMAAALGEEVSEEEAAAILATADTDGDGLLDHHE 61

Query: 97  FERL-----LEVGEEEKLKDLKEAFALY-DCEGCGFITPKNLKKMLRKLG---ERKSTEE 156
           F RL     L+   EE L+ L+EAF +Y + E    ITP +L++MLR+LG   +R   EE
Sbjct: 62  FMRLSAAHQLQEPAEESLRCLREAFDMYAEEEETAVITPASLRRMLRRLGSEHQRLEMEE 121

Query: 157 CKLMIRRFDLNGDGLISFEEFQIMM 172
           C+ MI RFDLNGDG++SF+EF++MM
Sbjct: 122 CRAMICRFDLNGDGVLSFDEFRVMM 146

[Close]

BLAST of Bhi02G000399 vs. NCBI nr
Match: XP_038879778.1 (putative calcium-binding protein CML19 [Benincasa hispida])

HSP 1 Score: 344.0 bits (881), Expect = 7.6e-91
Identity = 172/172 (100.00%), Postives = 172/172 (100.00%), Query Frame = 0


Query: 1   METPNPELTKNPNSEKNPQNSFLKILQSRIEMEINCAAQYDRVLSYFDEDGDGKISPSEL 60
           METPNPELTKNPNSEKNPQNSFLKILQSRIEMEINCAAQYDRVLSYFDEDGDGKISPSEL
Sbjct: 1   METPNPELTKNPNSEKNPQNSFLKILQSRIEMEINCAAQYDRVLSYFDEDGDGKISPSEL 60

Query: 61  RNRLGLMGGELLQAEAEAAVESLDSDGDGLLCAGDFERLLEVGEEEKLKDLKEAFALYDC 120
           RNRLGLMGGELLQAEAEAAVESLDSDGDGLLCAGDFERLLEVGEEEKLKDLKEAFALYDC
Sbjct: 61  RNRLGLMGGELLQAEAEAAVESLDSDGDGLLCAGDFERLLEVGEEEKLKDLKEAFALYDC 120

Query: 121 EGCGFITPKNLKKMLRKLGERKSTEECKLMIRRFDLNGDGLISFEEFQIMMA 173
           EGCGFITPKNLKKMLRKLGERKSTEECKLMIRRFDLNGDGLISFEEFQIMMA
Sbjct: 121 EGCGFITPKNLKKMLRKLGERKSTEECKLMIRRFDLNGDGLISFEEFQIMMA 172

[Close]

BLAST of Bhi02G000399 vs. NCBI nr
Match: KAG7023071.1 (putative calcium-binding protein CML19, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 276.6 bits (706), Expect = 1.5e-70
Identity = 141/167 (84.43%), Postives = 150/167 (89.82%), Query Frame = 0


Query: 6   PELTKNPNSEKNPQNSFLKILQSRIEMEINCAAQYDRVLSYFDEDGDGKISPSELRNRLG 65
           PELTK+PNS K+      +I QSRIEMEIN +AQY RVLSYFDEDGDGKISPSELRNR+G
Sbjct: 2   PELTKHPNSRKS------EIFQSRIEMEINSSAQYGRVLSYFDEDGDGKISPSELRNRVG 61

Query: 66  LMGGELLQAEAEAAVESLDSDGDGLLCAGDFERLLEVGEEEKLKDLKEAFALYDCEGCGF 125
           LMGGELL  EAE AVESLDSDGDGLLCAGDFERLL+VGEEEKLKDLKEAF LYDCEGCGF
Sbjct: 62  LMGGELLLGEAEMAVESLDSDGDGLLCAGDFERLLDVGEEEKLKDLKEAFGLYDCEGCGF 121

Query: 126 ITPKNLKKMLRKLGERKSTEECKLMIRRFDLNGDGLISFEEFQIMMA 173
           ITPK+LK+MLRKLGE KST ECKLMI +FDLNGDGLISFEEFQIMMA
Sbjct: 122 ITPKSLKRMLRKLGESKSTAECKLMIGQFDLNGDGLISFEEFQIMMA 162

[Close]

BLAST of Bhi02G000399 vs. NCBI nr
Match: XP_022921811.1 (putative calcium-binding protein CML19 [Cucurbita moschata])

HSP 1 Score: 275.4 bits (703), Expect = 3.3e-70
Identity = 140/167 (83.83%), Postives = 150/167 (89.82%), Query Frame = 0


Query: 6   PELTKNPNSEKNPQNSFLKILQSRIEMEINCAAQYDRVLSYFDEDGDGKISPSELRNRLG 65
           PELTK+PNS K+      ++ QSRIEMEIN +AQY RVLSYFDEDGDGKISPSELRNR+G
Sbjct: 5   PELTKHPNSRKS------EVFQSRIEMEINNSAQYGRVLSYFDEDGDGKISPSELRNRVG 64

Query: 66  LMGGELLQAEAEAAVESLDSDGDGLLCAGDFERLLEVGEEEKLKDLKEAFALYDCEGCGF 125
           LMGGELL  EAE AVESLDSDGDGLLCAGDFERLL+VGEEEKLKDLKEAF LYDCEGCGF
Sbjct: 65  LMGGELLLGEAEMAVESLDSDGDGLLCAGDFERLLDVGEEEKLKDLKEAFGLYDCEGCGF 124

Query: 126 ITPKNLKKMLRKLGERKSTEECKLMIRRFDLNGDGLISFEEFQIMMA 173
           ITPK+LK+MLRKLGE KST ECKLMI +FDLNGDGLISFEEFQIMMA
Sbjct: 125 ITPKSLKRMLRKLGESKSTAECKLMIGQFDLNGDGLISFEEFQIMMA 165

[Close]

BLAST of Bhi02G000399 vs. NCBI nr
Match: XP_008464450.1 (PREDICTED: putative calcium-binding protein CML19 [Cucumis melo] >KAA0057778.1 putative calcium-binding protein CML19 [Cucumis melo var. makuwa] >TYJ98459.1 putative calcium-binding protein CML19 [Cucumis melo var. makuwa])

HSP 1 Score: 266.2 bits (679), Expect = 2.0e-67
Identity = 135/142 (95.07%), Postives = 137/142 (96.48%), Query Frame = 0


Query: 32  MEIN-CAAQYDRVLSYFDEDGDGKISPSELRNRLGLMGGELLQAEAEAAVESLDSDGDGL 91
           MEIN   AQYDRVLSYFDEDGDGKISPSELRNRLGL+GGELLQAEAEAAVESLDSDGDGL
Sbjct: 1   MEINKSGAQYDRVLSYFDEDGDGKISPSELRNRLGLIGGELLQAEAEAAVESLDSDGDGL 60

Query: 92  LCAGDFERLLEVGEEEKLKDLKEAFALYDCEGCGFITPKNLKKMLRKLGERKSTEECKLM 151
           LCAGD ERLLEVGEEEKLKDLKEAFALYD EGCGFITPKNLKKMLRKLGERKSTEECK+M
Sbjct: 61  LCAGDIERLLEVGEEEKLKDLKEAFALYDSEGCGFITPKNLKKMLRKLGERKSTEECKMM 120

Query: 152 IRRFDLNGDGLISFEEFQIMMA 173
           IRRFDLNGDGLISFEEFQIMMA
Sbjct: 121 IRRFDLNGDGLISFEEFQIMMA 142

[Close]

BLAST of Bhi02G000399 vs. NCBI nr
Match: KAE8652382.1 (hypothetical protein Csa_014061 [Cucumis sativus])

HSP 1 Score: 261.2 bits (666), Expect = 6.5e-66
Identity = 131/148 (88.51%), Postives = 138/148 (93.24%), Query Frame = 0


Query: 25  ILQSRIEMEINCAAQYDRVLSYFDEDGDGKISPSELRNRLGLMGGELLQAEAEAAVESLD 84
           +L + +E+ +   AQYDRVLSYFDEDGDGKISPSELRNRLGL+GGEL QAEAEAAVESLD
Sbjct: 46  VLNTDLEI-VKSGAQYDRVLSYFDEDGDGKISPSELRNRLGLIGGELQQAEAEAAVESLD 105

Query: 85  SDGDGLLCAGDFERLLEVGEEEKLKDLKEAFALYDCEGCGFITPKNLKKMLRKLGERKST 144
           SDGDGLLC GD ERLLEVGEEEKLKDLKEAFALYD EGCGFITPKNLKKMLRKLGERKST
Sbjct: 106 SDGDGLLCVGDIERLLEVGEEEKLKDLKEAFALYDSEGCGFITPKNLKKMLRKLGERKST 165

Query: 145 EECKLMIRRFDLNGDGLISFEEFQIMMA 173
           EECK+MIRRFDLNGDGLISFEEFQIMMA
Sbjct: 166 EECKMMIRRFDLNGDGLISFEEFQIMMA 192

[Close]

BLAST of Bhi02G000399 vs. ExPASy TrEMBL
Match: A0A6J1E2E7 (putative calcium-binding protein CML19 OS=Cucurbita moschata OX=3662 GN=LOC111429954 PE=4 SV=1)

HSP 1 Score: 275.4 bits (703), Expect = 1.6e-70
Identity = 140/167 (83.83%), Postives = 150/167 (89.82%), Query Frame = 0


Query: 6   PELTKNPNSEKNPQNSFLKILQSRIEMEINCAAQYDRVLSYFDEDGDGKISPSELRNRLG 65
           PELTK+PNS K+      ++ QSRIEMEIN +AQY RVLSYFDEDGDGKISPSELRNR+G
Sbjct: 5   PELTKHPNSRKS------EVFQSRIEMEINNSAQYGRVLSYFDEDGDGKISPSELRNRVG 64

Query: 66  LMGGELLQAEAEAAVESLDSDGDGLLCAGDFERLLEVGEEEKLKDLKEAFALYDCEGCGF 125
           LMGGELL  EAE AVESLDSDGDGLLCAGDFERLL+VGEEEKLKDLKEAF LYDCEGCGF
Sbjct: 65  LMGGELLLGEAEMAVESLDSDGDGLLCAGDFERLLDVGEEEKLKDLKEAFGLYDCEGCGF 124

Query: 126 ITPKNLKKMLRKLGERKSTEECKLMIRRFDLNGDGLISFEEFQIMMA 173
           ITPK+LK+MLRKLGE KST ECKLMI +FDLNGDGLISFEEFQIMMA
Sbjct: 125 ITPKSLKRMLRKLGESKSTAECKLMIGQFDLNGDGLISFEEFQIMMA 165

[Close]

BLAST of Bhi02G000399 vs. ExPASy TrEMBL
Match: A0A5A7URJ2 (Putative calcium-binding protein CML19 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold350G00610 PE=4 SV=1)

HSP 1 Score: 266.2 bits (679), Expect = 9.8e-68
Identity = 135/142 (95.07%), Postives = 137/142 (96.48%), Query Frame = 0


Query: 32  MEIN-CAAQYDRVLSYFDEDGDGKISPSELRNRLGLMGGELLQAEAEAAVESLDSDGDGL 91
           MEIN   AQYDRVLSYFDEDGDGKISPSELRNRLGL+GGELLQAEAEAAVESLDSDGDGL
Sbjct: 1   MEINKSGAQYDRVLSYFDEDGDGKISPSELRNRLGLIGGELLQAEAEAAVESLDSDGDGL 60

Query: 92  LCAGDFERLLEVGEEEKLKDLKEAFALYDCEGCGFITPKNLKKMLRKLGERKSTEECKLM 151
           LCAGD ERLLEVGEEEKLKDLKEAFALYD EGCGFITPKNLKKMLRKLGERKSTEECK+M
Sbjct: 61  LCAGDIERLLEVGEEEKLKDLKEAFALYDSEGCGFITPKNLKKMLRKLGERKSTEECKMM 120

Query: 152 IRRFDLNGDGLISFEEFQIMMA 173
           IRRFDLNGDGLISFEEFQIMMA
Sbjct: 121 IRRFDLNGDGLISFEEFQIMMA 142

[Close]

BLAST of Bhi02G000399 vs. ExPASy TrEMBL
Match: A0A1S3CN12 (putative calcium-binding protein CML19 OS=Cucumis melo OX=3656 GN=LOC103502330 PE=4 SV=1)

HSP 1 Score: 266.2 bits (679), Expect = 9.8e-68
Identity = 135/142 (95.07%), Postives = 137/142 (96.48%), Query Frame = 0


Query: 32  MEIN-CAAQYDRVLSYFDEDGDGKISPSELRNRLGLMGGELLQAEAEAAVESLDSDGDGL 91
           MEIN   AQYDRVLSYFDEDGDGKISPSELRNRLGL+GGELLQAEAEAAVESLDSDGDGL
Sbjct: 1   MEINKSGAQYDRVLSYFDEDGDGKISPSELRNRLGLIGGELLQAEAEAAVESLDSDGDGL 60

Query: 92  LCAGDFERLLEVGEEEKLKDLKEAFALYDCEGCGFITPKNLKKMLRKLGERKSTEECKLM 151
           LCAGD ERLLEVGEEEKLKDLKEAFALYD EGCGFITPKNLKKMLRKLGERKSTEECK+M
Sbjct: 61  LCAGDIERLLEVGEEEKLKDLKEAFALYDSEGCGFITPKNLKKMLRKLGERKSTEECKMM 120

Query: 152 IRRFDLNGDGLISFEEFQIMMA 173
           IRRFDLNGDGLISFEEFQIMMA
Sbjct: 121 IRRFDLNGDGLISFEEFQIMMA 142

[Close]

BLAST of Bhi02G000399 vs. ExPASy TrEMBL
Match: A0A0A0LU40 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G002110 PE=4 SV=1)

HSP 1 Score: 260.4 bits (664), Expect = 5.4e-66
Identity = 129/139 (92.81%), Postives = 132/139 (94.96%), Query Frame = 0


Query: 34  INCAAQYDRVLSYFDEDGDGKISPSELRNRLGLMGGELLQAEAEAAVESLDSDGDGLLCA 93
           +   AQYDRVLSYFDEDGDGKISPSELRNRLGL+GGEL QAEAEAAVESLDSDGDGLLC 
Sbjct: 4   VKSGAQYDRVLSYFDEDGDGKISPSELRNRLGLIGGELQQAEAEAAVESLDSDGDGLLCV 63

Query: 94  GDFERLLEVGEEEKLKDLKEAFALYDCEGCGFITPKNLKKMLRKLGERKSTEECKLMIRR 153
           GD ERLLEVGEEEKLKDLKEAFALYD EGCGFITPKNLKKMLRKLGERKSTEECK+MIRR
Sbjct: 64  GDIERLLEVGEEEKLKDLKEAFALYDSEGCGFITPKNLKKMLRKLGERKSTEECKMMIRR 123

Query: 154 FDLNGDGLISFEEFQIMMA 173
           FDLNGDGLISFEEFQIMMA
Sbjct: 124 FDLNGDGLISFEEFQIMMA 142

[Close]

BLAST of Bhi02G000399 vs. ExPASy TrEMBL
Match: A0A6J1JE84 (putative calcium-binding protein CML19 OS=Cucurbita maxima OX=3661 GN=LOC111484995 PE=4 SV=1)

HSP 1 Score: 255.0 bits (650), Expect = 2.3e-64
Identity = 128/141 (90.78%), Postives = 131/141 (92.91%), Query Frame = 0


Query: 32  MEINCAAQYDRVLSYFDEDGDGKISPSELRNRLGLMGGELLQAEAEAAVESLDSDGDGLL 91
           MEIN +A Y RVLSYFDEDGDGKISPSELRNR+GLMGGELL  EAE AVESLDSDGDGLL
Sbjct: 1   MEINSSAHYGRVLSYFDEDGDGKISPSELRNRVGLMGGELLLGEAEMAVESLDSDGDGLL 60

Query: 92  CAGDFERLLEVGEEEKLKDLKEAFALYDCEGCGFITPKNLKKMLRKLGERKSTEECKLMI 151
           CAGDFERLLEVGEEEKLKDLKEAFALYDCEGCGFITPK+LKKMLRKLGERKST ECKLMI
Sbjct: 61  CAGDFERLLEVGEEEKLKDLKEAFALYDCEGCGFITPKSLKKMLRKLGERKSTAECKLMI 120

Query: 152 RRFDLNGDGLISFEEFQIMMA 173
             FDLNGDGLISFEEFQ MMA
Sbjct: 121 GVFDLNGDGLISFEEFQKMMA 141

The following BLAST results are available for this feature:

BLAST of Bhi02G000399 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G76650.1	4.7e-30	49.25	calmodulin-like 38	[more]
AT1G76650.3	4.7e-30	49.25	calmodulin-like 38	[more]
AT1G76640.1	1.2e-28	41.10	Calcium-binding EF-hand family protein	[more]
AT1G76650.2	1.1e-26	46.27	calmodulin-like 38	[more]
AT5G42380.1	7.0e-26	45.45	calmodulin like 37	[more]

BLAST of Bhi02G000399 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q8RYJ8	3.5e-30	52.45	Putative calcium-binding protein CML19 OS=Oryza sativa subsp. japonica OX=39947 ...	[more]
Q9SRE6	6.6e-29	49.25	Calcium-binding protein CML38 OS=Arabidopsis thaliana OX=3702 GN=CML38 PE=1 SV=1	[more]
Q8RYK0	5.6e-28	49.62	Probable calcium-binding protein CML31 OS=Oryza sativa subsp. japonica OX=39947 ...	[more]
Q9SRE7	1.6e-27	41.10	Calcium-binding protein CML39 OS=Arabidopsis thaliana OX=3702 GN=CML39 PE=2 SV=1	[more]
Q2QYW1	3.4e-25	46.90	Probable calcium-binding protein CML25/26 OS=Oryza sativa subsp. japonica OX=399...	[more]

BLAST of Bhi02G000399 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_038879778.1	7.6e-91	100.00	putative calcium-binding protein CML19 [Benincasa hispida]	[more]
KAG7023071.1	1.5e-70	84.43	putative calcium-binding protein CML19, partial [Cucurbita argyrosperma subsp. a...	[more]
XP_022921811.1	3.3e-70	83.83	putative calcium-binding protein CML19 [Cucurbita moschata]	[more]
XP_008464450.1	2.0e-67	95.07	PREDICTED: putative calcium-binding protein CML19 [Cucumis melo] >KAA0057778.1 p...	[more]
KAE8652382.1	6.5e-66	88.51	hypothetical protein Csa_014061 [Cucumis sativus]	[more]

BLAST of Bhi02G000399 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1E2E7	1.6e-70	83.83	putative calcium-binding protein CML19 OS=Cucurbita moschata OX=3662 GN=LOC11142...	[more]
A0A5A7URJ2	9.8e-68	95.07	Putative calcium-binding protein CML19 OS=Cucumis melo var. makuwa OX=1194695 GN...	[more]
A0A1S3CN12	9.8e-68	95.07	putative calcium-binding protein CML19 OS=Cucumis melo OX=3656 GN=LOC103502330 P...	[more]
A0A0A0LU40	5.4e-66	92.81	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G002110 PE=4 SV=1	[more]
A0A6J1JE84	2.3e-64	90.78	putative calcium-binding protein CML19 OS=Cucurbita maxima OX=3661 GN=LOC1114849...	[more]

InterPro

Analysis Name: InterPro Annotations of Wax gourd (B227) v1
Date Performed: 2021-10-22


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR002048	EF-hand domain	SMART	SM00054	efh_1	coord: 75..103 e-value: 45.0 score: 4.4 coord: 39..67 e-value: 0.13 score: 21.4 coord: 146..172 e-value: 0.2 score: 20.8 coord: 110..138 e-value: 0.047 score: 22.8
IPR002048	EF-hand domain	PFAM	PF13499	EF-hand_7	coord: 109..171 e-value: 5.0E-15 score: 55.7
IPR002048	EF-hand domain	PFAM	PF13202	EF-hand_5	coord: 43..62 e-value: 2.1E-5 score: 23.8
IPR002048	EF-hand domain	PROSITE	PS50222	EF_HAND_2	coord: 142..172 score: 14.318138
IPR002048	EF-hand domain	PROSITE	PS50222	EF_HAND_2	coord: 35..70 score: 11.500702
IPR002048	EF-hand domain	PROSITE	PS50222	EF_HAND_2	coord: 106..141 score: 12.086506
IPR002048	EF-hand domain	CDD	cd00051	EFh	coord: 110..172 e-value: 7.06973E-16 score: 66.4173
None	No IPR available	GENE3D	1.10.238.10		coord: 101..172 e-value: 3.3E-22 score: 80.5
None	No IPR available	GENE3D	1.10.238.10		coord: 7..100 e-value: 9.1E-12 score: 47.2
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 1..20
None	No IPR available	PANTHER	PTHR10891:SF870	EF HAND CALCIUM-BINDING FAMILY PROTEIN	coord: 37..172
None	No IPR available	PANTHER	PTHR10891	EF-HAND CALCIUM-BINDING DOMAIN CONTAINING PROTEIN	coord: 37..172
IPR018247	EF-Hand 1, calcium-binding site	PROSITE	PS00018	EF_HAND_1	coord: 155..167
IPR018247	EF-Hand 1, calcium-binding site	PROSITE	PS00018	EF_HAND_1	coord: 48..60
IPR011992	EF-hand domain pair	SUPERFAMILY	47473	EF-hand	coord: 35..171

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Bhi02M000399	Bhi02M000399	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
molecular_function	GO:0005509	calcium ion binding