Bhi02G000389 (gene) Wax gourd (B227) v1

Overview
NameBhi02G000389
Typegene
OrganismBenincasa hispida (Wax gourd (B227) v1)
DescriptionRNA-binding protein CP31B, chloroplastic
Locationchr2: 9898927 .. 9899546 (-)
RNA-Seq ExpressionBhi02G000389
SyntenyBhi02G000389
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGCAGACGTTATTCATTTCAGCAGCTTTTCAGACCTATACCTCTGATGGCTCGTCCTCTCTATCTCCGGCAGCTCATCCTCTCTATCTCCATCGTCGACTCCTTCCAGCGCGAGCTTCCTACCAACTCCATCAAGATCTGAGAGATCGAATCCTTCCAGCATCGTCGAGCTTTATTTCAGGTTTGTTCCAGATCTAAAAGTTGTTCTCTTCACACATTTCAGGTTTATTTTAGGTCAAACAAAAAGTTGAAGAAGAAATTAAGAGAAAAAGCTAGCCGAATCATTGATTTCCTCAGTCGCCGGAAAAGACTCCAACGGTGATGTTTTTGATGAACTGGACATAGAATCTCGGCGACAATCCTTCAAAGAGCTATTAGGCACTGCTAGTCGCCGTGGACCCAGATTGTCTAAGCAAGAGAAACGAAGAGAATCACTCATTTTGATAGCTCCGAATGGGAATAAGAATGAGAAGAAGGAAGATGGGAATTGGGGATTGGGAGATATGAAGAGGGGGAGGAAGAGGAAGAGAGATGAAGGGGTGAAAGAAAAGAATGTGGTTGATGGGTTGGAAGAGAATAATGAGAAGGCCAAAAAGTTGAAGAAAAAGAAGCAACAATAG

mRNA sequence

ATGCAGACGTTATTCATTTCAGCAGCTTTTCAGACCTATACCTCTGATGGCTCGTCCTCTCTATCTCCGGCAGCTCATCCTCTCTATCTCCATCGTCGACTCCTTCCAGCGCGAGCTTCCTACCAACTCCATCAAGATCTGAGAGATCGAATCCTTCCAGCATCGTCGAGCTTTATTTCAGTCGCCGGAAAAGACTCCAACGGTGATGTTTTTGATGAACTGGACATAGAATCTCGGCGACAATCCTTCAAAGAGCTATTAGGCACTGCTAGTCGCCGTGGACCCAGATTGTCTAAGCAAGAGAAACGAAGAGAATCACTCATTTTGATAGCTCCGAATGGGAATAAGAATGAGAAGAAGGAAGATGGGAATTGGGGATTGGGAGATATGAAGAGGGGGAGGAAGAGGAAGAGAGATGAAGGGGTGAAAGAAAAGAATGTGGTTGATGGGTTGGAAGAGAATAATGAGAAGGCCAAAAAGTTGAAGAAAAAGAAGCAACAATAG

Coding sequence (CDS)

ATGCAGACGTTATTCATTTCAGCAGCTTTTCAGACCTATACCTCTGATGGCTCGTCCTCTCTATCTCCGGCAGCTCATCCTCTCTATCTCCATCGTCGACTCCTTCCAGCGCGAGCTTCCTACCAACTCCATCAAGATCTGAGAGATCGAATCCTTCCAGCATCGTCGAGCTTTATTTCAGTCGCCGGAAAAGACTCCAACGGTGATGTTTTTGATGAACTGGACATAGAATCTCGGCGACAATCCTTCAAAGAGCTATTAGGCACTGCTAGTCGCCGTGGACCCAGATTGTCTAAGCAAGAGAAACGAAGAGAATCACTCATTTTGATAGCTCCGAATGGGAATAAGAATGAGAAGAAGGAAGATGGGAATTGGGGATTGGGAGATATGAAGAGGGGGAGGAAGAGGAAGAGAGATGAAGGGGTGAAAGAAAAGAATGTGGTTGATGGGTTGGAAGAGAATAATGAGAAGGCCAAAAAGTTGAAGAAAAAGAAGCAACAATAG

Protein sequence

MQTLFISAAFQTYTSDGSSSLSPAAHPLYLHRRLLPARASYQLHQDLRDRILPASSSFISVAGKDSNGDVFDELDIESRRQSFKELLGTASRRGPRLSKQEKRRESLILIAPNGNKNEKKEDGNWGLGDMKRGRKRKRDEGVKEKNVVDGLEENNEKAKKLKKKKQQ
Homology
BLAST of Bhi02G000389 vs. NCBI nr
Match: XP_038891335.1 (protein gar2 [Benincasa hispida])

HSP 1 Score: 156.8 bits (395), Expect = 1.7e-34
Identity = 91/126 (72.22%), Postives = 104/126 (82.54%), Query Frame = 0

Query: 42  QLHQDLRDRILPASSSFISVAGKDSNGDVFDELDIESRRQSFKELLGTASRRGPRLSKQE 101
           +L + LR+++  A S   SVAGKDSNGDV  E D ESRR+S KELLGT  R GPRLSK+E
Sbjct: 7   KLKKKLREKL--AESLISSVAGKDSNGDVSGEPDTESRRKSLKELLGTVCRHGPRLSKRE 66

Query: 102 KRRESLILIAPNGNKNEKKEDGNWGLGDMKRGRKRKRDEGVKEKNVVDGLEENNEKAKKL 161
           KRRESLIL A +GNK+EKKEDGN GLG+ KR +KRKRDEG KEKN+VDGLEENNEKAKKL
Sbjct: 67  KRRESLILTASDGNKDEKKEDGNRGLGETKREKKRKRDEGAKEKNLVDGLEENNEKAKKL 126

Query: 162 KKKKQQ 168
           KKKK+Q
Sbjct: 127 KKKKKQ 130

BLAST of Bhi02G000389 vs. NCBI nr
Match: XP_008461639.1 (PREDICTED: RNA-binding protein CP31B, chloroplastic [Cucumis melo])

HSP 1 Score: 142.1 bits (357), Expect = 4.3e-30
Identity = 85/126 (67.46%), Postives = 100/126 (79.37%), Query Frame = 0

Query: 42  QLHQDLRDRILPASSSFISVAGKDSNGDVFDELDIESRRQSFKELLGTASRRGPRLSKQE 101
           +L Q LR+++  A S   SVAGKD+N DV  E D ES R+S KELLGTAS  GPRLSK+E
Sbjct: 7   KLKQKLREKL--AESLISSVAGKDTNVDVSGERDTESPRKSLKELLGTASGHGPRLSKRE 66

Query: 102 KRRESLILIAPNGNKNEKKEDGNWGLGDMKRGRKRKRDEGVKEKNVVDGLEENNEKAKKL 161
           KRRESLIL   +GNKNEKKE+ N GLG+MKR +KRKR+EGVKEKN VDGLE++ EKAKKL
Sbjct: 67  KRRESLILTGSDGNKNEKKEEENQGLGEMKREKKRKRNEGVKEKNAVDGLEKDKEKAKKL 126

Query: 162 KKKKQQ 168
           K KK+Q
Sbjct: 127 KNKKKQ 130

BLAST of Bhi02G000389 vs. NCBI nr
Match: XP_031742307.1 (protein gar2 [Cucumis sativus] >KAE8647980.1 hypothetical protein Csa_000404 [Cucumis sativus])

HSP 1 Score: 130.6 bits (327), Expect = 1.3e-26
Identity = 81/126 (64.29%), Postives = 96/126 (76.19%), Query Frame = 0

Query: 42  QLHQDLRDRILPASSSFISVAGKDSNGDVFDELDIESRRQSFKELLGTASRRGPRLSKQE 101
           +L Q LR+++  A S   SVAG+D+N  V  E D ES R+S KELLGTAS  GPRLSK+E
Sbjct: 7   KLKQKLREKL--AESLISSVAGRDTNFGVSGERDTESPRKSLKELLGTASGNGPRLSKRE 66

Query: 102 KRRESLILIAPNGNKNEKKEDGNWGLGDMKRGRKRKRDEGVKEKNVVDGLEENNEKAKKL 161
           KRRESL+L   +GNK EKKED N GLG+    +KRKR+EGVKEKN VDGLEE+NEKAKKL
Sbjct: 67  KRRESLVLTGSDGNKKEKKEDENQGLGE----KKRKRNEGVKEKNAVDGLEEDNEKAKKL 126

Query: 162 KKKKQQ 168
           K KK+Q
Sbjct: 127 KNKKKQ 126

BLAST of Bhi02G000389 vs. NCBI nr
Match: XP_022139342.1 (protein gar2 [Momordica charantia])

HSP 1 Score: 114.8 bits (286), Expect = 7.3e-22
Identity = 76/127 (59.84%), Postives = 89/127 (70.08%), Query Frame = 0

Query: 42  QLHQDLRDRILPASSSFISVAGKDSNGDVFDELDIESRRQSFKELLGTASRRGPRLSKQE 101
           +L Q LR+++  A S   +VAGKDS GDV  E D ESR QS +ELLG  S  GPRLSK+E
Sbjct: 7   KLKQKLREKL--AESLIATVAGKDSVGDVSGEPDSESRPQSLRELLGAVSHHGPRLSKRE 66

Query: 102 KRRESLILIAPN-GNKNEKKEDGNWGLGDMKRGRKRKRDEGVKEKNVVDGLEENNEKAKK 161
           KRRESL L+A +  N  EKKED N  LG  KR  KRKRDE   EK+ +DG  ENNEKAKK
Sbjct: 67  KRRESLTLVASDEKNNEEKKEDENQRLGGTKREXKRKRDEEGAEKSALDGSAENNEKAKK 126

Query: 162 LKKKKQQ 168
           LKKKK++
Sbjct: 127 LKKKKKK 131

BLAST of Bhi02G000389 vs. NCBI nr
Match: XP_023517053.1 (protein gar2 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 111.7 bits (278), Expect = 6.2e-21
Identity = 74/127 (58.27%), Postives = 90/127 (70.87%), Query Frame = 0

Query: 42  QLHQDLRDRILPASSSFISVAGKDSNGDVFDELDIESRRQSFKELLGTASRRGPRLSKQE 101
           +L Q  R+++  A S   S+AGK SNGDV  E + +SR QS KELLG   R G RLSK+E
Sbjct: 7   KLKQKFREKL--AESLISSIAGKVSNGDVSGEPESDSRPQSLKELLGGTGRHGTRLSKRE 66

Query: 102 KRRESLILIAPNG-NKNEKKEDGNWGLGDMKRGRKRKRDEGVKEKNVVDGLEENNEKAKK 161
           KRRES+I  A +G N  EKKE  N  LG+ KR +KRKRDE VKEK+ VDGLEE++EKAKK
Sbjct: 67  KRRESVISAASDGKNDEEKKEGENQRLGERKREKKRKRDEEVKEKSAVDGLEEDDEKAKK 126

Query: 162 LKKKKQQ 168
           LK KK +
Sbjct: 127 LKLKKNK 131

BLAST of Bhi02G000389 vs. ExPASy TrEMBL
Match: A0A1S3CF29 (RNA-binding protein CP31B, chloroplastic OS=Cucumis melo OX=3656 GN=LOC103500194 PE=4 SV=1)

HSP 1 Score: 142.1 bits (357), Expect = 2.1e-30
Identity = 85/126 (67.46%), Postives = 100/126 (79.37%), Query Frame = 0

Query: 42  QLHQDLRDRILPASSSFISVAGKDSNGDVFDELDIESRRQSFKELLGTASRRGPRLSKQE 101
           +L Q LR+++  A S   SVAGKD+N DV  E D ES R+S KELLGTAS  GPRLSK+E
Sbjct: 7   KLKQKLREKL--AESLISSVAGKDTNVDVSGERDTESPRKSLKELLGTASGHGPRLSKRE 66

Query: 102 KRRESLILIAPNGNKNEKKEDGNWGLGDMKRGRKRKRDEGVKEKNVVDGLEENNEKAKKL 161
           KRRESLIL   +GNKNEKKE+ N GLG+MKR +KRKR+EGVKEKN VDGLE++ EKAKKL
Sbjct: 67  KRRESLILTGSDGNKNEKKEEENQGLGEMKREKKRKRNEGVKEKNAVDGLEKDKEKAKKL 126

Query: 162 KKKKQQ 168
           K KK+Q
Sbjct: 127 KNKKKQ 130

BLAST of Bhi02G000389 vs. ExPASy TrEMBL
Match: A0A0A0KLT8 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G180870 PE=4 SV=1)

HSP 1 Score: 130.6 bits (327), Expect = 6.2e-27
Identity = 81/126 (64.29%), Postives = 96/126 (76.19%), Query Frame = 0

Query: 42  QLHQDLRDRILPASSSFISVAGKDSNGDVFDELDIESRRQSFKELLGTASRRGPRLSKQE 101
           +L Q LR+++  A S   SVAG+D+N  V  E D ES R+S KELLGTAS  GPRLSK+E
Sbjct: 7   KLKQKLREKL--AESLISSVAGRDTNFGVSGERDTESPRKSLKELLGTASGNGPRLSKRE 66

Query: 102 KRRESLILIAPNGNKNEKKEDGNWGLGDMKRGRKRKRDEGVKEKNVVDGLEENNEKAKKL 161
           KRRESL+L   +GNK EKKED N GLG+    +KRKR+EGVKEKN VDGLEE+NEKAKKL
Sbjct: 67  KRRESLVLTGSDGNKKEKKEDENQGLGE----KKRKRNEGVKEKNAVDGLEEDNEKAKKL 126

Query: 162 KKKKQQ 168
           K KK+Q
Sbjct: 127 KNKKKQ 126

BLAST of Bhi02G000389 vs. ExPASy TrEMBL
Match: A0A6J1CDP4 (protein gar2 OS=Momordica charantia OX=3673 GN=LOC111010281 PE=4 SV=1)

HSP 1 Score: 114.8 bits (286), Expect = 3.5e-22
Identity = 76/127 (59.84%), Postives = 89/127 (70.08%), Query Frame = 0

Query: 42  QLHQDLRDRILPASSSFISVAGKDSNGDVFDELDIESRRQSFKELLGTASRRGPRLSKQE 101
           +L Q LR+++  A S   +VAGKDS GDV  E D ESR QS +ELLG  S  GPRLSK+E
Sbjct: 7   KLKQKLREKL--AESLIATVAGKDSVGDVSGEPDSESRPQSLRELLGAVSHHGPRLSKRE 66

Query: 102 KRRESLILIAPN-GNKNEKKEDGNWGLGDMKRGRKRKRDEGVKEKNVVDGLEENNEKAKK 161
           KRRESL L+A +  N  EKKED N  LG  KR  KRKRDE   EK+ +DG  ENNEKAKK
Sbjct: 67  KRRESLTLVASDEKNNEEKKEDENQRLGGTKREXKRKRDEEGAEKSALDGSAENNEKAKK 126

Query: 162 LKKKKQQ 168
           LKKKK++
Sbjct: 127 LKKKKKK 131

BLAST of Bhi02G000389 vs. ExPASy TrEMBL
Match: A0A6J1FSC3 (protein gar2 OS=Cucurbita moschata OX=3662 GN=LOC111447996 PE=4 SV=1)

HSP 1 Score: 107.1 bits (266), Expect = 7.4e-20
Identity = 72/127 (56.69%), Postives = 89/127 (70.08%), Query Frame = 0

Query: 42  QLHQDLRDRILPASSSFISVAGKDSNGDVFDELDIESRRQSFKELLGTASRRGPRLSKQE 101
           +L Q  R+++  A S   S+AGK SNGDV  E + +SR QS KELLG   R G RLSK+E
Sbjct: 7   KLKQKFREKL--AESLISSIAGKVSNGDVSGEPESDSRPQSLKELLGGTGRHGTRLSKRE 66

Query: 102 KRRESLILIAPNG-NKNEKKEDGNWGLGDMKRGRKRKRDEGVKEKNVVDGLEENNEKAKK 161
           KRRES+I  A +G N  EKKE  N  LG+ K  +KRKR+E VKEK+ VDGLEE++EKAKK
Sbjct: 67  KRRESVISTASDGKNDEEKKEGENQRLGERKGEKKRKRNEEVKEKSAVDGLEEDDEKAKK 126

Query: 162 LKKKKQQ 168
           LK KK +
Sbjct: 127 LKLKKNK 131

BLAST of Bhi02G000389 vs. ExPASy TrEMBL
Match: A0A6J1JA04 (protein gar2-like OS=Cucurbita maxima OX=3661 GN=LOC111483911 PE=4 SV=1)

HSP 1 Score: 106.7 bits (265), Expect = 9.6e-20
Identity = 71/125 (56.80%), Postives = 87/125 (69.60%), Query Frame = 0

Query: 42  QLHQDLRDRILPASSSFISVAGKDSNGDVFDELDIESRRQSFKELLGTASRRGPRLSKQE 101
           +L Q  R+++  A S   S+AG+ SNGDV  E + +SR QS KELLG   R G RLSK+E
Sbjct: 7   KLKQKFREKL--AESLISSIAGRVSNGDVSGEPESDSRPQSLKELLGGTGRHGTRLSKRE 66

Query: 102 KRRESLILIAPNG-NKNEKKEDGNWGLGDMKRGRKRKRDEGVKEKNVVDGLEENNEKAKK 161
           KRRES I  A +G N  EKKE  N  LG+ K  +KRKR+E VKEK+ VDGLEE++EKAKK
Sbjct: 67  KRRESFISTASDGKNDEEKKEGENQRLGERKGEKKRKRNEEVKEKSAVDGLEEDDEKAKK 126

Query: 162 LKKKK 166
           LKK K
Sbjct: 127 LKKNK 129

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Match NameE-valueIdentityDescription
Match NameE-valueIdentityDescription
XP_038891335.11.7e-3472.22protein gar2 [Benincasa hispida][more]
XP_008461639.14.3e-3067.46PREDICTED: RNA-binding protein CP31B, chloroplastic [Cucumis melo][more]
XP_031742307.11.3e-2664.29protein gar2 [Cucumis sativus] >KAE8647980.1 hypothetical protein Csa_000404 [Cu... [more]
XP_022139342.17.3e-2259.84protein gar2 [Momordica charantia][more]
XP_023517053.16.2e-2158.27protein gar2 [Cucurbita pepo subsp. pepo][more]
Match NameE-valueIdentityDescription
A0A1S3CF292.1e-3067.46RNA-binding protein CP31B, chloroplastic OS=Cucumis melo OX=3656 GN=LOC103500194... [more]
A0A0A0KLT86.2e-2764.29Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G180870 PE=4 SV=1[more]
A0A6J1CDP43.5e-2259.84protein gar2 OS=Momordica charantia OX=3673 GN=LOC111010281 PE=4 SV=1[more]
A0A6J1FSC37.4e-2056.69protein gar2 OS=Cucurbita moschata OX=3662 GN=LOC111447996 PE=4 SV=1[more]
A0A6J1JA049.6e-2056.80protein gar2-like OS=Cucurbita maxima OX=3661 GN=LOC111483911 PE=4 SV=1[more]
InterPro
Analysis Name: InterPro Annotations of Wax gourd (B227) v1
Date Performed: 2021-10-22
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 148..167
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 87..167
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 115..160

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Bhi02M000389Bhi02M000389mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0003723 RNA binding
molecular_function GO:0008270 zinc ion binding