Bhi02G000332 (gene) Wax gourd (B227) v1

BLAST of Bhi02G000332 vs. TAIR 10
Match: AT1G70370.1 (polygalacturonase 2 )

HSP 1 Score: 702.2 bits (1811), Expect = 3.7e-202
Identity = 365/624 (58.49%), Postives = 442/624 (70.83%), Query Frame = 0

Query: 7   FLLLSQLSLSFFHV------SFASADHQQSNPFTAKASLLRYWNSHVSNQLPPPDFLFSK 66
           FLLL   S   F+V      +F      + NPFT KASL+RYWN  +  Q P  +FL SK
Sbjct: 5   FLLLQSFSFFLFNVVIVGGRTFGGGFSAEENPFTPKASLVRYWNKEIRGQSPRSEFLISK 64

Query: 67  ASPLSAVDAAVLAQHAARGSLSFRLESFCYSANLFCSFDSETEISVPRLKDADFKLYGNK 126
           ASPL+AVD+A  ++ AA  SL  R   FC +ANLFC  D    +      D  F +Y  K
Sbjct: 65  ASPLNAVDSATFSKLAAANSLPTRFPDFCSAANLFCFPDLGASLE-KHDDDVKFSVYDQK 124

Query: 127 NFVNYGGARLGGTDSFKNYSDGLNTADHSFKRYGGNSSNHEEGFTNYAEDGNVANSNFIS 186
           NF NYG AR GG DSFKNYS   N    SF+RY  N++ H++ FT Y E+ NV    F S
Sbjct: 125 NFTNYGNARAGGADSFKNYSKDGNVVTDSFRRYSRNAAGHDDKFTVYGENSNVVEEGFNS 184

Query: 187 YATESKGGSGEFVNYDKLVNVPNLRFTAYDLNSRNHKLSFTGYGNETNSGS-QTFTSYGK 246
           Y T   GG+G+F NY   VN P  RFTAY         +F  Y +E N+G+ Q+FTSYGK
Sbjct: 185 YGTFGTGGAGDFTNYQNNVNNPTSRFTAYSDGGNGRSQTFKTYTHEANAGNGQSFTSYGK 244

Query: 247 KGNGVPQEFSSYSDGSNILTSTFTGYGESGKTANDSFKGYGVSGNNPHTNFKNYGKGGNS 306
            GNGVP EF+SY   SN++ S F+ YGESG  AND+F  YG  GN P  NF NYG  GN+
Sbjct: 245 NGNGVPNEFTSYGVSSNVIGSGFSNYGESGNAANDTFTSYGSDGNVPQNNFNNYGASGNA 304

Query: 307 EIDTFSNYRNGANVGDDSFQSYAKNSNSGRVNFANYGKSFNHGNDSFTEYGKGSIGRTTV 366
            +DTF+NYR+ ANVGDDSF SYAK+SNS +VNF NYG+SFN G+++FT YGKG+ G + +
Sbjct: 305 AVDTFANYRDKANVGDDSFSSYAKDSNSEKVNFVNYGQSFNPGSETFTGYGKGAEG-SKL 364

Query: 367 GFKSYSVDRNFTTYAQKGVSFSEYSNFTT---ATGSGIHVNKLVEPGKFFRESELKQGNV 426
            FK+Y+ +  F  YA+KGV+F++Y+  TT     G G  VNK +EPGKFFRES LK+G V
Sbjct: 365 SFKTYTPNSTFKDYAKKGVAFAKYNVSTTTANTVGDGKTVNKWIEPGKFFRESSLKEGTV 424

Query: 427 MVMPDIRDKMPPRSFLPRPILVNLPFSSSRISEAKEIFHARDGSTMERVITNALGECERA 486
           + MPDI+DKMP RSFLPR I+  LPFS+S++ E K IFHA + STM  +IT+A+ ECER 
Sbjct: 425 IPMPDIKDKMPKRSFLPRSIITKLPFSTSKLGEIKRIFHAVENSTMGGIITDAVTECERP 484

Query: 487 PSPGETKRCVGSAEDMIDFATTVLGRRVVARTTANVNGSREKVEIGTVKGINGGDLTSSV 546
           PS GETKRCVGSAEDMIDFAT+VLGR VV RTT NV GS+EKV IG V GINGG LT +V
Sbjct: 485 PSVGETKRCVGSAEDMIDFATSVLGRSVVLRTTENVAGSKEKVVIGKVNGINGGKLTKAV 544

Query: 547 SCHQSLYPYLLYYCHSVPKVRIYEADILRLKTKEKINRGVAICHMDTSSWGPEHGAFVAL 606
           SCHQSLYPYLLYYCHSVPKVR+YEAD+L L +K+KIN G+AICHMDTSSWGP HGAF+AL
Sbjct: 545 SCHQSLYPYLLYYCHSVPKVRVYEADLLELNSKKKINHGIAICHMDTSSWGPSHGAFLAL 604

Query: 607 GSGPGQIEVCHWIFENDMSWSVAD 621
           GS PG+IEVCHWIFENDM+W++AD
Sbjct: 605 GSKPGRIEVCHWIFENDMNWAIAD 626

BLAST of Bhi02G000332 vs. TAIR 10
Match: AT1G70370.2 (polygalacturonase 2 )

HSP 1 Score: 702.2 bits (1811), Expect = 3.7e-202
Identity = 365/624 (58.49%), Postives = 442/624 (70.83%), Query Frame = 0

Query: 7   FLLLSQLSLSFFHV------SFASADHQQSNPFTAKASLLRYWNSHVSNQLPPPDFLFSK 66
           FLLL   S   F+V      +F      + NPFT KASL+RYWN  +  Q P  +FL SK
Sbjct: 5   FLLLQSFSFFLFNVVIVGGRTFGGGFSAEENPFTPKASLVRYWNKEIRGQSPRSEFLISK 64

Query: 67  ASPLSAVDAAVLAQHAARGSLSFRLESFCYSANLFCSFDSETEISVPRLKDADFKLYGNK 126
           ASPL+AVD+A  ++ AA  SL  R   FC +ANLFC  D    +      D  F +Y  K
Sbjct: 65  ASPLNAVDSATFSKLAAANSLPTRFPDFCSAANLFCFPDLGASLE-KHDDDVKFSVYDQK 124

Query: 127 NFVNYGGARLGGTDSFKNYSDGLNTADHSFKRYGGNSSNHEEGFTNYAEDGNVANSNFIS 186
           NF NYG AR GG DSFKNYS   N    SF+RY  N++ H++ FT Y E+ NV    F S
Sbjct: 125 NFTNYGNARAGGADSFKNYSKDGNVVTDSFRRYSRNAAGHDDKFTVYGENSNVVEEGFNS 184

Query: 187 YATESKGGSGEFVNYDKLVNVPNLRFTAYDLNSRNHKLSFTGYGNETNSGS-QTFTSYGK 246
           Y T   GG+G+F NY   VN P  RFTAY         +F  Y +E N+G+ Q+FTSYGK
Sbjct: 185 YGTFGTGGAGDFTNYQNNVNNPTSRFTAYSDGGNGRSQTFKTYTHEANAGNGQSFTSYGK 244

Query: 247 KGNGVPQEFSSYSDGSNILTSTFTGYGESGKTANDSFKGYGVSGNNPHTNFKNYGKGGNS 306
            GNGVP EF+SY   SN++ S F+ YGESG  AND+F  YG  GN P  NF NYG  GN+
Sbjct: 245 NGNGVPNEFTSYGVSSNVIGSGFSNYGESGNAANDTFTSYGSDGNVPQNNFNNYGASGNA 304

Query: 307 EIDTFSNYRNGANVGDDSFQSYAKNSNSGRVNFANYGKSFNHGNDSFTEYGKGSIGRTTV 366
            +DTF+NYR+ ANVGDDSF SYAK+SNS +VNF NYG+SFN G+++FT YGKG+ G + +
Sbjct: 305 AVDTFANYRDKANVGDDSFSSYAKDSNSEKVNFVNYGQSFNPGSETFTGYGKGAEG-SKL 364

Query: 367 GFKSYSVDRNFTTYAQKGVSFSEYSNFTT---ATGSGIHVNKLVEPGKFFRESELKQGNV 426
            FK+Y+ +  F  YA+KGV+F++Y+  TT     G G  VNK +EPGKFFRES LK+G V
Sbjct: 365 SFKTYTPNSTFKDYAKKGVAFAKYNVSTTTANTVGDGKTVNKWIEPGKFFRESSLKEGTV 424

Query: 427 MVMPDIRDKMPPRSFLPRPILVNLPFSSSRISEAKEIFHARDGSTMERVITNALGECERA 486
           + MPDI+DKMP RSFLPR I+  LPFS+S++ E K IFHA + STM  +IT+A+ ECER 
Sbjct: 425 IPMPDIKDKMPKRSFLPRSIITKLPFSTSKLGEIKRIFHAVENSTMGGIITDAVTECERP 484

Query: 487 PSPGETKRCVGSAEDMIDFATTVLGRRVVARTTANVNGSREKVEIGTVKGINGGDLTSSV 546
           PS GETKRCVGSAEDMIDFAT+VLGR VV RTT NV GS+EKV IG V GINGG LT +V
Sbjct: 485 PSVGETKRCVGSAEDMIDFATSVLGRSVVLRTTENVAGSKEKVVIGKVNGINGGKLTKAV 544

Query: 547 SCHQSLYPYLLYYCHSVPKVRIYEADILRLKTKEKINRGVAICHMDTSSWGPEHGAFVAL 606
           SCHQSLYPYLLYYCHSVPKVR+YEAD+L L +K+KIN G+AICHMDTSSWGP HGAF+AL
Sbjct: 545 SCHQSLYPYLLYYCHSVPKVRVYEADLLELNSKKKINHGIAICHMDTSSWGPSHGAFLAL 604

Query: 607 GSGPGQIEVCHWIFENDMSWSVAD 621
           GS PG+IEVCHWIFENDM+W++AD
Sbjct: 605 GSKPGRIEVCHWIFENDMNWAIAD 626

BLAST of Bhi02G000332 vs. TAIR 10
Match: AT1G23760.1 (BURP domain-containing protein )

HSP 1 Score: 669.1 bits (1725), Expect = 3.5e-192
Identity = 353/623 (56.66%), Postives = 432/623 (69.34%), Query Frame = 0

Query: 6   FFLLLSQLSLSFFHVSFASADH-------QQSNPFTAKASLLRYWNSHVSNQLPPPDFLF 65
           F  LL  LS   +HV  A  +         + NPFT KASL+RYWN  + +Q P   FL 
Sbjct: 5   FVFLLPFLS-RLYHVVIAGGERLTNGGFSPEINPFTPKASLVRYWNKQIHHQSPGSAFLL 64

Query: 66  SKASPLSAVDAAVLAQHAARGSLSFRLESFCYSANLFCSFDSETEISVPRLKDADFKLYG 125
           SKASPL+AV +A  A+ A+  +L  RL  FC +ANLFC  D    +      D  F +Y 
Sbjct: 65  SKASPLTAVASATFAKLASENALPDRLPDFCSAANLFCFPDLGPTLE-KHSNDVKFSVYD 124

Query: 126 NKNFVNYGGARLGGTDSFKNYSDGLNTADHSFKRYGGNSSNHEEGFTNYAEDGNVANSNF 185
            +NF NYG    GG DSFK YS   N    SF+RY  +++ HE+ FT YA++ NV    F
Sbjct: 125 QRNFTNYGTIHPGGADSFKKYSQNGNAVTDSFRRYSRDAAGHEDKFTGYADNSNVVEEKF 184

Query: 186 ISYATESKGGSGEFVNYDKLVNVPNLRFTAYDLNSRNHKLSFTGYGNETNSG-SQTFTSY 245
            SY T + GGSG+F NY   VN P  RF AY         +F  Y +E NSG  Q+FTSY
Sbjct: 185 NSYGTSATGGSGDFKNYQSGVNNPTTRFKAYSDEGNGRAQTFKTYTHEANSGPGQSFTSY 244

Query: 246 GKKGNGVPQEFSSYSDGSNILTSTFTGYGESGKTANDSFKGYGVSGNNPHTNFKNYGKGG 305
           GK GNG P EFSSY   SN++ S F+ YGE+G  AND+FKGYG  GN P  NFK+YG  G
Sbjct: 245 GKNGNGAPNEFSSYGVSSNVIGSDFSNYGENGNAANDTFKGYGGDGNVPQNNFKSYGASG 304

Query: 306 NSEIDTFSNYRNGANVGDDSFQSYAKNSNSGRVNFANYGKSFNHGNDSFTEYGKGSIGRT 365
           N+ +DTF NYR+ ANVGDDSF SYAKNSN  +V+F NYGKS N G++SF+ YGKG+ G  
Sbjct: 305 NAAVDTFLNYRDNANVGDDSFSSYAKNSNFEKVDFVNYGKSINPGSESFSGYGKGAEG-N 364

Query: 366 TVGFKSYSVDRNFTTYAQKGVSFSEYSNFTTATGSGIHVNKLVEPGKFFRESELKQGNVM 425
            + FK+Y+ +  F  Y + GV F++Y+   ++ G G  VNK VEPGKFFRES LK+G ++
Sbjct: 365 KIDFKTYTQNSTFKDYTKTGVEFAKYNR--SSLGGGKTVNKWVEPGKFFRESMLKEGTLI 424

Query: 426 VMPDIRDKMPPRSFLPRPILVNLPFSSSRISEAKEIFHARDGSTMERVITNALGECERAP 485
            MPDI+DKMP RSFLPR I+  LPFS+S+I+E K +FHA D STME +IT+A+ ECER P
Sbjct: 425 WMPDIKDKMPKRSFLPRSIVSKLPFSTSKIAEIKRVFHANDNSTMEGIITDAVRECERPP 484

Query: 486 SPGETKRCVGSAEDMIDFATTVLGRRVVARTTANVNGSREKVEIGTVKGINGGDLTSSVS 545
           +  ETKRCVGSAEDMIDFAT+VLGR VV RTT +V GS+EKV IG V GINGG +T SVS
Sbjct: 485 TVSETKRCVGSAEDMIDFATSVLGRSVVLRTTESVAGSKEKVMIGKVNGINGGRVTKSVS 544

Query: 546 CHQSLYPYLLYYCHSVPKVRIYEADILRLKTKEKINRGVAICHMDTSSWGPEHGAFVALG 605
           CHQSLYPYLLYYCHSVPKVR+YE+D+L  K+K KIN G+AICHMDTS+WG  HGAF+ LG
Sbjct: 545 CHQSLYPYLLYYCHSVPKVRVYESDLLDPKSKAKINHGIAICHMDTSAWGANHGAFMLLG 604

Query: 606 SGPGQIEVCHWIFENDMSWSVAD 621
           S PGQIEVCHWIFENDM+W++AD
Sbjct: 605 SRPGQIEVCHWIFENDMNWAIAD 622

BLAST of Bhi02G000332 vs. TAIR 10
Match: AT1G60390.1 (polygalacturonase 1 )

HSP 1 Score: 664.5 bits (1713), Expect = 8.6e-191
Identity = 346/618 (55.99%), Postives = 429/618 (69.42%), Query Frame = 0

Query: 4   IVFFLLLSQLSLSFFHVSFASADHQQS-NPFTAKASLLRYWNSHVSNQLPPPDFLFSKAS 63
           + FF + S  ++ F      + +   S NPFT KASL+RYWN+H++   P P F  SKAS
Sbjct: 10  LCFFCIFSSSNVHFAGAKQTAGNITPSENPFTPKASLIRYWNNHINGDSPKPSFFLSKAS 69

Query: 64  PLSAVDAAVLAQHAARGSLSFRLESFCYSANLFCSFDSETEISVPRLKDADFKLYGNKNF 123
           PL+AVD+   A  A+  +L+     FC +A LFC  +           D +F  Y  KNF
Sbjct: 70  PLTAVDSTRFASLASNHALNTHHSDFCSAAKLFCFPELAAHSLEKHGDDVNFAAYSGKNF 129

Query: 124 VNYGGARLGGTDSFKNYSDGLNTADHSFKRYGGNSSNHEEGFTNYAEDGNVANSNFISYA 183
            NYG  RL G DSFKNYS G N A  SF+RY  NS+ H++GFTNYA + NVA+ +F +YA
Sbjct: 130 TNYGSDRLSGADSFKNYSGGDNIAVDSFRRYSRNSAGHDDGFTNYAGEVNVADQSFTTYA 189

Query: 184 TESKGGSGEFVNYDKLVNVPNLRFTAYDLNSRNHKLSFTGYGNETNSGSQTFTSYGKKGN 243
           T + GGSGEF NY+   N PN RFT+Y   +     +FT Y    N+G Q+FTSY K GN
Sbjct: 190 TGTTGGSGEFTNYNTDANEPNGRFTSYSDKANGRSQTFTTYSENGNTGYQSFTSYSKNGN 249

Query: 244 GVPQEFSSYSDGSNILTSTFTGYGESGKTANDSFKGYGVSGNNPHTNFKNYGKGGNSEID 303
           G P EFS Y  GSN++ + FT YGES   ANDSF  YG +GN P   FK YG GGN  + 
Sbjct: 250 GAPNEFSGYGTGSNVVNTGFTKYGESANGANDSFTSYGENGNVPVNEFKGYGDGGNGAVY 309

Query: 304 TFSNYRNGANVGDDSFQSYAKNSNSGRVNFANYGKSFNHGNDSFTEYGKGSIGRTTVGFK 363
            F NYR+ +N+G DSF SYAKNSN+ +VNF NYGKSFN G+D+FT YG+ ++G   V FK
Sbjct: 310 GFKNYRDQSNIGVDSFSSYAKNSNNEKVNFVNYGKSFNLGSDNFTGYGQDNVG-GNVSFK 369

Query: 364 SYSVDRNFTTYAQKGVSFSEYSNFTTATGSGIHVNKLVEPGKFFRESELKQGNVMVMPDI 423
           +Y   ++F  Y + GV F+ YSN    + +G  VNK VE GKFFRE+ LK+G +M MPDI
Sbjct: 370 TYGQGQSFKVYTKDGVVFARYSN--NVSSNGKTVNKWVEEGKFFREAMLKEGTLMQMPDI 429

Query: 424 RDKMPPRSFLPRPILVNLPFSSSRISEAKEIFHARDGSTMERVITNALGECERAPSPGET 483
           +DKMP R+FLPR I+ NLPFSSS I E   +F A + S+M  +I++A+ ECER  S GET
Sbjct: 430 KDKMPKRTFLPRNIVKNLPFSSSTIGEIWRVFGAGENSSMAGIISSAVSECERPASHGET 489

Query: 484 KRCVGSAEDMIDFATTVLGRRVVARTTANVNGSREKVEIGTVKGINGGDLTSSVSCHQSL 543
           KRCVGSAEDMIDFAT+VLGR VV RTT NV GS++KV IG V GINGGD+T +VSCHQSL
Sbjct: 490 KRCVGSAEDMIDFATSVLGRGVVVRTTENVVGSKKKVVIGKVNGINGGDVTRAVSCHQSL 549

Query: 544 YPYLLYYCHSVPKVRIYEADILRLKTKEKINRGVAICHMDTSSWGPEHGAFVALGSGPGQ 603
           YPYLLYYCHSVP+VR+YE D+L  K+ EKIN GVAICH+DTS+W P HGAF+ALGSGPGQ
Sbjct: 550 YPYLLYYCHSVPRVRVYETDLLDPKSLEKINHGVAICHIDTSAWSPSHGAFLALGSGPGQ 609

Query: 604 IEVCHWIFENDMSWSVAD 621
           IEVCHWIFENDM+W++ D
Sbjct: 610 IEVCHWIFENDMTWNIID 624

BLAST of Bhi02G000332 vs. TAIR 10
Match: AT5G25610.1 (BURP domain-containing protein )

HSP 1 Score: 115.5 bits (288), Expect = 1.5e-25
Identity = 71/218 (32.57%), Postives = 107/218 (49.08%), Query Frame = 0

Query: 405 FFRESELKQGNVM-VMPDIRDKMPPR-SFLPRPILVNLPFSSSRISEAKEIFHARDGSTM 464
           FF E +L +G  M V  +  D    + +FLPR     +PF S + SE  + F    GS  
Sbjct: 176 FFLEKDLVRGKEMNVRFNAEDGYGGKTAFLPRGEAETVPFGSEKFSETLKRFSVEAGSEE 235

Query: 465 ERVITNALGECERAPSPGETKRCVGSAEDMIDFATTVLGR---RVVARTTANVNGSREKV 524
             ++   + ECE     GE K C  S E M+DF+ + LG+   R V+   A  N   +K 
Sbjct: 236 AEMMKKTIEECEARKVSGEEKYCATSLESMVDFSVSKLGKYHVRAVSTEVAKKNAPMQKY 295

Query: 525 EIGTVKGINGGDLTSSVSCHQSLYPYLLYYCHSVPKVRIYEADILRLKTKEKIN-RGVAI 584
           +I    G+       SV CH+  YP+ ++YCH      +Y    + L+ +  +  + VA+
Sbjct: 296 KIAAA-GVKKLSDDKSVVCHKQKYPFAVFYCHKAMMTTVY---AVPLEGENGMRAKAVAV 355

Query: 585 CHMDTSSWGPEHGAFVALGSGPGQIEVCHWIFENDMSW 617
           CH +TS+W P H AF  L   PG + VCH++ E  + W
Sbjct: 356 CHKNTSAWNPNHLAFKVLKVKPGTVPVCHFLPETHVVW 389

BLAST of Bhi02G000332 vs. ExPASy Swiss-Prot
Match: P92990 (Polygalacturonase 1 beta-like protein 3 OS=Arabidopsis thaliana OX=3702 GN=PGL3 PE=2 SV=2)

HSP 1 Score: 702.2 bits (1811), Expect = 5.2e-201
Identity = 365/624 (58.49%), Postives = 442/624 (70.83%), Query Frame = 0

Query: 7   FLLLSQLSLSFFHV------SFASADHQQSNPFTAKASLLRYWNSHVSNQLPPPDFLFSK 66
           FLLL   S   F+V      +F      + NPFT KASL+RYWN  +  Q P  +FL SK
Sbjct: 5   FLLLQSFSFFLFNVVIVGGRTFGGGFSAEENPFTPKASLVRYWNKEIRGQSPRSEFLISK 64

Query: 67  ASPLSAVDAAVLAQHAARGSLSFRLESFCYSANLFCSFDSETEISVPRLKDADFKLYGNK 126
           ASPL+AVD+A  ++ AA  SL  R   FC +ANLFC  D    +      D  F +Y  K
Sbjct: 65  ASPLNAVDSATFSKLAAANSLPTRFPDFCSAANLFCFPDLGASLE-KHDDDVKFSVYDQK 124

Query: 127 NFVNYGGARLGGTDSFKNYSDGLNTADHSFKRYGGNSSNHEEGFTNYAEDGNVANSNFIS 186
           NF NYG AR GG DSFKNYS   N    SF+RY  N++ H++ FT Y E+ NV    F S
Sbjct: 125 NFTNYGNARAGGADSFKNYSKDGNVVTDSFRRYSRNAAGHDDKFTVYGENSNVVEEGFNS 184

Query: 187 YATESKGGSGEFVNYDKLVNVPNLRFTAYDLNSRNHKLSFTGYGNETNSGS-QTFTSYGK 246
           Y T   GG+G+F NY   VN P  RFTAY         +F  Y +E N+G+ Q+FTSYGK
Sbjct: 185 YGTFGTGGAGDFTNYQNNVNNPTSRFTAYSDGGNGRSQTFKTYTHEANAGNGQSFTSYGK 244

Query: 247 KGNGVPQEFSSYSDGSNILTSTFTGYGESGKTANDSFKGYGVSGNNPHTNFKNYGKGGNS 306
            GNGVP EF+SY   SN++ S F+ YGESG  AND+F  YG  GN P  NF NYG  GN+
Sbjct: 245 NGNGVPNEFTSYGVSSNVIGSGFSNYGESGNAANDTFTSYGSDGNVPQNNFNNYGASGNA 304

Query: 307 EIDTFSNYRNGANVGDDSFQSYAKNSNSGRVNFANYGKSFNHGNDSFTEYGKGSIGRTTV 366
            +DTF+NYR+ ANVGDDSF SYAK+SNS +VNF NYG+SFN G+++FT YGKG+ G + +
Sbjct: 305 AVDTFANYRDKANVGDDSFSSYAKDSNSEKVNFVNYGQSFNPGSETFTGYGKGAEG-SKL 364

Query: 367 GFKSYSVDRNFTTYAQKGVSFSEYSNFTT---ATGSGIHVNKLVEPGKFFRESELKQGNV 426
            FK+Y+ +  F  YA+KGV+F++Y+  TT     G G  VNK +EPGKFFRES LK+G V
Sbjct: 365 SFKTYTPNSTFKDYAKKGVAFAKYNVSTTTANTVGDGKTVNKWIEPGKFFRESSLKEGTV 424

Query: 427 MVMPDIRDKMPPRSFLPRPILVNLPFSSSRISEAKEIFHARDGSTMERVITNALGECERA 486
           + MPDI+DKMP RSFLPR I+  LPFS+S++ E K IFHA + STM  +IT+A+ ECER 
Sbjct: 425 IPMPDIKDKMPKRSFLPRSIITKLPFSTSKLGEIKRIFHAVENSTMGGIITDAVTECERP 484

Query: 487 PSPGETKRCVGSAEDMIDFATTVLGRRVVARTTANVNGSREKVEIGTVKGINGGDLTSSV 546
           PS GETKRCVGSAEDMIDFAT+VLGR VV RTT NV GS+EKV IG V GINGG LT +V
Sbjct: 485 PSVGETKRCVGSAEDMIDFATSVLGRSVVLRTTENVAGSKEKVVIGKVNGINGGKLTKAV 544

Query: 547 SCHQSLYPYLLYYCHSVPKVRIYEADILRLKTKEKINRGVAICHMDTSSWGPEHGAFVAL 606
           SCHQSLYPYLLYYCHSVPKVR+YEAD+L L +K+KIN G+AICHMDTSSWGP HGAF+AL
Sbjct: 545 SCHQSLYPYLLYYCHSVPKVRVYEADLLELNSKKKINHGIAICHMDTSSWGPSHGAFLAL 604

Query: 607 GSGPGQIEVCHWIFENDMSWSVAD 621
           GS PG+IEVCHWIFENDM+W++AD
Sbjct: 605 GSKPGRIEVCHWIFENDMNWAIAD 626

BLAST of Bhi02G000332 vs. ExPASy Swiss-Prot
Match: P92982 (Polygalacturonase 1 beta-like protein 1 OS=Arabidopsis thaliana OX=3702 GN=PGL1 PE=2 SV=2)

HSP 1 Score: 669.1 bits (1725), Expect = 4.9e-191
Identity = 353/623 (56.66%), Postives = 432/623 (69.34%), Query Frame = 0

Query: 6   FFLLLSQLSLSFFHVSFASADH-------QQSNPFTAKASLLRYWNSHVSNQLPPPDFLF 65
           F  LL  LS   +HV  A  +         + NPFT KASL+RYWN  + +Q P   FL 
Sbjct: 5   FVFLLPFLS-RLYHVVIAGGERLTNGGFSPEINPFTPKASLVRYWNKQIHHQSPGSAFLL 64

Query: 66  SKASPLSAVDAAVLAQHAARGSLSFRLESFCYSANLFCSFDSETEISVPRLKDADFKLYG 125
           SKASPL+AV +A  A+ A+  +L  RL  FC +ANLFC  D    +      D  F +Y 
Sbjct: 65  SKASPLTAVASATFAKLASENALPDRLPDFCSAANLFCFPDLGPTLE-KHSNDVKFSVYD 124

Query: 126 NKNFVNYGGARLGGTDSFKNYSDGLNTADHSFKRYGGNSSNHEEGFTNYAEDGNVANSNF 185
            +NF NYG    GG DSFK YS   N    SF+RY  +++ HE+ FT YA++ NV    F
Sbjct: 125 QRNFTNYGTIHPGGADSFKKYSQNGNAVTDSFRRYSRDAAGHEDKFTGYADNSNVVEEKF 184

Query: 186 ISYATESKGGSGEFVNYDKLVNVPNLRFTAYDLNSRNHKLSFTGYGNETNSG-SQTFTSY 245
            SY T + GGSG+F NY   VN P  RF AY         +F  Y +E NSG  Q+FTSY
Sbjct: 185 NSYGTSATGGSGDFKNYQSGVNNPTTRFKAYSDEGNGRAQTFKTYTHEANSGPGQSFTSY 244

Query: 246 GKKGNGVPQEFSSYSDGSNILTSTFTGYGESGKTANDSFKGYGVSGNNPHTNFKNYGKGG 305
           GK GNG P EFSSY   SN++ S F+ YGE+G  AND+FKGYG  GN P  NFK+YG  G
Sbjct: 245 GKNGNGAPNEFSSYGVSSNVIGSDFSNYGENGNAANDTFKGYGGDGNVPQNNFKSYGASG 304

Query: 306 NSEIDTFSNYRNGANVGDDSFQSYAKNSNSGRVNFANYGKSFNHGNDSFTEYGKGSIGRT 365
           N+ +DTF NYR+ ANVGDDSF SYAKNSN  +V+F NYGKS N G++SF+ YGKG+ G  
Sbjct: 305 NAAVDTFLNYRDNANVGDDSFSSYAKNSNFEKVDFVNYGKSINPGSESFSGYGKGAEG-N 364

Query: 366 TVGFKSYSVDRNFTTYAQKGVSFSEYSNFTTATGSGIHVNKLVEPGKFFRESELKQGNVM 425
            + FK+Y+ +  F  Y + GV F++Y+   ++ G G  VNK VEPGKFFRES LK+G ++
Sbjct: 365 KIDFKTYTQNSTFKDYTKTGVEFAKYNR--SSLGGGKTVNKWVEPGKFFRESMLKEGTLI 424

Query: 426 VMPDIRDKMPPRSFLPRPILVNLPFSSSRISEAKEIFHARDGSTMERVITNALGECERAP 485
            MPDI+DKMP RSFLPR I+  LPFS+S+I+E K +FHA D STME +IT+A+ ECER P
Sbjct: 425 WMPDIKDKMPKRSFLPRSIVSKLPFSTSKIAEIKRVFHANDNSTMEGIITDAVRECERPP 484

Query: 486 SPGETKRCVGSAEDMIDFATTVLGRRVVARTTANVNGSREKVEIGTVKGINGGDLTSSVS 545
           +  ETKRCVGSAEDMIDFAT+VLGR VV RTT +V GS+EKV IG V GINGG +T SVS
Sbjct: 485 TVSETKRCVGSAEDMIDFATSVLGRSVVLRTTESVAGSKEKVMIGKVNGINGGRVTKSVS 544

Query: 546 CHQSLYPYLLYYCHSVPKVRIYEADILRLKTKEKINRGVAICHMDTSSWGPEHGAFVALG 605
           CHQSLYPYLLYYCHSVPKVR+YE+D+L  K+K KIN G+AICHMDTS+WG  HGAF+ LG
Sbjct: 545 CHQSLYPYLLYYCHSVPKVRVYESDLLDPKSKAKINHGIAICHMDTSAWGANHGAFMLLG 604

Query: 606 SGPGQIEVCHWIFENDMSWSVAD 621
           S PGQIEVCHWIFENDM+W++AD
Sbjct: 605 SRPGQIEVCHWIFENDMNWAIAD 622

BLAST of Bhi02G000332 vs. ExPASy Swiss-Prot
Match: O80760 (Polygalacturonase 1 beta-like protein 2 OS=Arabidopsis thaliana OX=3702 GN=PGL2 PE=2 SV=1)

HSP 1 Score: 664.5 bits (1713), Expect = 1.2e-189
Identity = 346/618 (55.99%), Postives = 429/618 (69.42%), Query Frame = 0

Query: 4   IVFFLLLSQLSLSFFHVSFASADHQQS-NPFTAKASLLRYWNSHVSNQLPPPDFLFSKAS 63
           + FF + S  ++ F      + +   S NPFT KASL+RYWN+H++   P P F  SKAS
Sbjct: 10  LCFFCIFSSSNVHFAGAKQTAGNITPSENPFTPKASLIRYWNNHINGDSPKPSFFLSKAS 69

Query: 64  PLSAVDAAVLAQHAARGSLSFRLESFCYSANLFCSFDSETEISVPRLKDADFKLYGNKNF 123
           PL+AVD+   A  A+  +L+     FC +A LFC  +           D +F  Y  KNF
Sbjct: 70  PLTAVDSTRFASLASNHALNTHHSDFCSAAKLFCFPELAAHSLEKHGDDVNFAAYSGKNF 129

Query: 124 VNYGGARLGGTDSFKNYSDGLNTADHSFKRYGGNSSNHEEGFTNYAEDGNVANSNFISYA 183
            NYG  RL G DSFKNYS G N A  SF+RY  NS+ H++GFTNYA + NVA+ +F +YA
Sbjct: 130 TNYGSDRLSGADSFKNYSGGDNIAVDSFRRYSRNSAGHDDGFTNYAGEVNVADQSFTTYA 189

Query: 184 TESKGGSGEFVNYDKLVNVPNLRFTAYDLNSRNHKLSFTGYGNETNSGSQTFTSYGKKGN 243
           T + GGSGEF NY+   N PN RFT+Y   +     +FT Y    N+G Q+FTSY K GN
Sbjct: 190 TGTTGGSGEFTNYNTDANEPNGRFTSYSDKANGRSQTFTTYSENGNTGYQSFTSYSKNGN 249

Query: 244 GVPQEFSSYSDGSNILTSTFTGYGESGKTANDSFKGYGVSGNNPHTNFKNYGKGGNSEID 303
           G P EFS Y  GSN++ + FT YGES   ANDSF  YG +GN P   FK YG GGN  + 
Sbjct: 250 GAPNEFSGYGTGSNVVNTGFTKYGESANGANDSFTSYGENGNVPVNEFKGYGDGGNGAVY 309

Query: 304 TFSNYRNGANVGDDSFQSYAKNSNSGRVNFANYGKSFNHGNDSFTEYGKGSIGRTTVGFK 363
            F NYR+ +N+G DSF SYAKNSN+ +VNF NYGKSFN G+D+FT YG+ ++G   V FK
Sbjct: 310 GFKNYRDQSNIGVDSFSSYAKNSNNEKVNFVNYGKSFNLGSDNFTGYGQDNVG-GNVSFK 369

Query: 364 SYSVDRNFTTYAQKGVSFSEYSNFTTATGSGIHVNKLVEPGKFFRESELKQGNVMVMPDI 423
           +Y   ++F  Y + GV F+ YSN    + +G  VNK VE GKFFRE+ LK+G +M MPDI
Sbjct: 370 TYGQGQSFKVYTKDGVVFARYSN--NVSSNGKTVNKWVEEGKFFREAMLKEGTLMQMPDI 429

Query: 424 RDKMPPRSFLPRPILVNLPFSSSRISEAKEIFHARDGSTMERVITNALGECERAPSPGET 483
           +DKMP R+FLPR I+ NLPFSSS I E   +F A + S+M  +I++A+ ECER  S GET
Sbjct: 430 KDKMPKRTFLPRNIVKNLPFSSSTIGEIWRVFGAGENSSMAGIISSAVSECERPASHGET 489

Query: 484 KRCVGSAEDMIDFATTVLGRRVVARTTANVNGSREKVEIGTVKGINGGDLTSSVSCHQSL 543
           KRCVGSAEDMIDFAT+VLGR VV RTT NV GS++KV IG V GINGGD+T +VSCHQSL
Sbjct: 490 KRCVGSAEDMIDFATSVLGRGVVVRTTENVVGSKKKVVIGKVNGINGGDVTRAVSCHQSL 549

Query: 544 YPYLLYYCHSVPKVRIYEADILRLKTKEKINRGVAICHMDTSSWGPEHGAFVALGSGPGQ 603
           YPYLLYYCHSVP+VR+YE D+L  K+ EKIN GVAICH+DTS+W P HGAF+ALGSGPGQ
Sbjct: 550 YPYLLYYCHSVPRVRVYETDLLDPKSLEKINHGVAICHIDTSAWSPSHGAFLALGSGPGQ 609

Query: 604 IEVCHWIFENDMSWSVAD 621
           IEVCHWIFENDM+W++ D
Sbjct: 610 IEVCHWIFENDMTWNIID 624

BLAST of Bhi02G000332 vs. ExPASy Swiss-Prot
Match: P93217 (Polygalacturonase non-catalytic subunit AroGP2 OS=Solanum lycopersicum OX=4081 GN=GP2 PE=3 SV=1)

HSP 1 Score: 641.3 bits (1653), Expect = 1.1e-182
Identity = 338/625 (54.08%), Postives = 421/625 (67.36%), Query Frame = 0

Query: 6   FFLLLSQLSLSFFHVSFASADHQQSNPFTAKASLLRYWNSHVSNQLPPPDFLFSKASPLS 65
           + LL+   SLS F++  A    +  NPFT K  ++RYWN HVSN LP P FL +KASPL+
Sbjct: 10  YILLVLLFSLSSFNIVVAKDGDESGNPFTPKGYVIRYWNKHVSNDLPKPWFLLNKASPLN 69

Query: 66  AVDAAVLAQHAA-RGSLSFRLESFCYSANLFCSFDSETEISVPRLKDADFKLYGNKNFVN 125
           A   A   +  A + +LS  L+SFC SANL C+ D    +      D  F  YGNKNF N
Sbjct: 70  AAQYATYTKLVADQNALSTHLQSFCSSANLMCAPDLLPSLE-KHTGDIHFTTYGNKNFTN 129

Query: 126 YGGARLG-GTDSFKNYSDGLNTADHSFKRYGGNSSNHEEGFTNYAEDGNVANSNFISYAT 185
           YG    G G ++FKNYS+  + + +SF+RY G  S  +  F NYA DGNV + +F SY+T
Sbjct: 130 YGTNEPGIGVNTFKNYSE--DASVNSFRRY-GRGSPRDNKFDNYAPDGNVIDQSFNSYST 189

Query: 186 ESKGGSGEFVNYDKLVNVPNLRFTAYDLNSRNHKLSFTGYGNETNSGSQTFTSYGKKGNG 245
            + GGSG+F NY    NVP+LRFTAY       +  F  Y  + NSG Q+F SYGK GNG
Sbjct: 190 NTPGGSGQFTNYAPNTNVPDLRFTAYSDQGTGGEQEFKTYLEQGNSGGQSFKSYGKNGNG 249

Query: 246 VPQEFSSYSDGSNILTSTFTGYGESGKTANDSFKGYGVSGNNPHTNFKNYGKGGNSEIDT 305
              +F+SY + +N+  STF  YG++    N +F  Y  +GNNP  NFKNYG GGN   +T
Sbjct: 250 ADSKFTSYGNETNVAASTFKNYGQNANGENQNFTSYSTNGNNPQNNFKNYGVGGNGPSET 309

Query: 306 FSNYRNGANVGDDSFQSYAKNSNSGRVNFANYGKSFNHGNDSFTEYGKGSIGRTTVGFKS 365
           F+NYR+ +NVGDD F +Y K++N+G  NF NYG+SFN G D F  YGKG      + FK+
Sbjct: 310 FTNYRDESNVGDDKFSNYVKDANAGEANFTNYGQSFNEGTDVFITYGKGG-NDPHINFKT 369

Query: 366 YSVDRNFTTYAQKGVSFSEYSNFTT--------ATGSGIHVNKLVEPGKFFRESELKQGN 425
           Y V+  F  Y +   +FS Y N T+          G     N+ +EPGKFFRE  LK G 
Sbjct: 370 YGVNNTFKDYVKDTATFSNYHNKTSQDLASLSEVNGGKKVNNRWIEPGKFFREKMLKSGT 429

Query: 426 VMVMPDIRDKMPPRSFLPRPILVNLPFSSSRISEAKEIFHARDGSTMERVITNALGECER 485
           +M MPDI+DKMP RSFLPR I   LPFS+S+I E K+IFHA + S + ++I +AL ECER
Sbjct: 430 IMPMPDIKDKMPKRSFLPRAIAAKLPFSTSKIDELKKIFHAANDSQVAKMIGDALSECER 489

Query: 486 APSPGETKRCVGSAEDMIDFATTVLGRRVVARTTANVNGSREKVEIGTVKGINGGDLTSS 545
           APSPGETK+CV SAEDMIDFAT+VLGR VV RTT N NGS+  + IG++KGINGG +T S
Sbjct: 490 APSPGETKQCVNSAEDMIDFATSVLGRNVVVRTTENTNGSKGNIMIGSIKGINGGKVTKS 549

Query: 546 VSCHQSLYPYLLYYCHSVPKVRIYEADILRLKTKEKINRGVAICHMDTSSWGPEHGAFVA 605
           VSCHQ+LYP LLYYCHSVPKVR+YEADIL   +K KIN GVAICH+DTSSWGP HGAF+A
Sbjct: 550 VSCHQTLYPSLLYYCHSVPKVRVYEADILDPNSKAKINHGVAICHVDTSSWGPRHGAFIA 609

Query: 606 LGSGPGQIEVCHWIFENDMSWSVAD 621
           LGSGPG+IEVCHWIFENDM+W+ AD
Sbjct: 610 LGSGPGKIEVCHWIFENDMTWATAD 629

BLAST of Bhi02G000332 vs. ExPASy Swiss-Prot
Match: P93218 (Polygalacturonase non-catalytic subunit AroGP3 OS=Solanum lycopersicum OX=4081 GN=GP3 PE=3 SV=1)

HSP 1 Score: 629.4 bits (1622), Expect = 4.3e-179
Identity = 334/624 (53.53%), Postives = 418/624 (66.99%), Query Frame = 0

Query: 8   LLLSQLSLSFFHVSFASADHQQSNPFTAKASLLRYWNSHVSNQLPPPDFLFSKASPLSAV 67
           LLL   +LS   V  A    +  NPFT K  L+RYWN HVSN LP P FL +KASPL+A 
Sbjct: 12  LLLLLFTLSSLDVVVAKDGDESGNPFTPKGYLIRYWNKHVSNDLPKPWFLLNKASPLNAA 71

Query: 68  DAAVLAQHAA-RGSLSFRLESFCYSANLFCSFDSETEISVPRLKDADFKLYGNKNFVNYG 127
             A   +  A + + +  L+SFC SANL C+ D    +   +  D  F  YG+KNF NYG
Sbjct: 72  QYATYTKLVADQNAFTTHLQSFCSSANLMCALDLLPSLEKHK-GDVHFVSYGDKNFTNYG 131

Query: 128 GARLG-GTDSFKNYSDGLNTADHSFKRYGGNSSNHEEGFTNY-AEDGNVANSNFISYATE 187
               G G +SFKNY++  N   +SF+RY G  S H+  F NY A  GN    +F SY+T 
Sbjct: 132 TKESGLGFNSFKNYTEEENFPVNSFRRY-GRDSPHDNQFDNYGAPGGNTPVQSFNSYSTN 191

Query: 188 SKGGSGEFVNYDKLVNVPNLRFTAYDLNSRNHKLSFTGYGNETNSGSQTFTSYGKKGNGV 247
           + GGSG+F NY    NVP+L FT+Y  +    +  F  YGN+ N+G Q+F SYGK GNG 
Sbjct: 192 TPGGSGQFTNYAPGSNVPDLHFTSYSDHGTGGEQDFKSYGNDGNAGQQSFKSYGKDGNGA 251

Query: 248 PQEFSSYSDGSNILTSTFTGYGESGKTANDSFKGYGVSGNNPHTNFKNYGKGGNSEIDTF 307
             +F++Y + +N+  S FT YGE+    N +F  YG +GNNP ++F NYG GGN   + F
Sbjct: 252 DSQFTTYGNNTNVDGSDFTNYGEAANGENQNFTSYGFNGNNPASSFNNYGVGGNGPSEAF 311

Query: 308 SNYRNGANVGDDSFQSYAKNSNSGRVNFANYGKSFNHGNDSFTEYGKGSIGRTTVGFKSY 367
           ++YR+ +NVGDD+F +Y K++N G  NF NYG+SFN G D FT YGKG      + FK+Y
Sbjct: 312 NSYRDQSNVGDDTFTTYVKDANGGEANFTNYGQSFNEGTDVFTTYGKGG-NDPHINFKTY 371

Query: 368 SVDRNFTTYAQKGVSFSEYSNFTT--------ATGSGIHVNKLVEPGKFFRESELKQGNV 427
            V+  F  Y +   +FS Y N T+          G     N+ +EPGKFFRE  LK G +
Sbjct: 372 GVNNTFKDYVKDTATFSNYHNKTSQDLASLSEVNGGKKVNNRWIEPGKFFREKMLKSGTI 431

Query: 428 MVMPDIRDKMPPRSFLPRPILVNLPFSSSRISEAKEIFHARDGSTMERVITNALGECERA 487
           M MPDI+DKMP RSFLPR I   LPFS+S+I E K+IFHA + S + ++I +AL ECERA
Sbjct: 432 MPMPDIKDKMPKRSFLPRAIAAKLPFSTSKIDELKKIFHAANDSQVAKMIGDALSECERA 491

Query: 488 PSPGETKRCVGSAEDMIDFATTVLGRRVVARTTANVNGSREKVEIGTVKGINGGDLTSSV 547
           PSPGETK+CV SAEDMIDFAT+VLGR VV RTT N NGS+  + IG++KGINGG +T SV
Sbjct: 492 PSPGETKQCVNSAEDMIDFATSVLGRNVVVRTTENTNGSKGNIMIGSIKGINGGKVTKSV 551

Query: 548 SCHQSLYPYLLYYCHSVPKVRIYEADILRLKTKEKINRGVAICHMDTSSWGPEHGAFVAL 607
           SCHQ+LYP LLYYCHSVPKVR+YEADIL   +K KIN GVAICH+DTSSWGP HGAFVAL
Sbjct: 552 SCHQTLYPSLLYYCHSVPKVRVYEADILDPNSKAKINHGVAICHVDTSSWGPRHGAFVAL 611

Query: 608 GSGPGQIEVCHWIFENDMSWSVAD 621
           GSGPG+IEVCHWIFENDM+W+ AD
Sbjct: 612 GSGPGKIEVCHWIFENDMTWATAD 632

BLAST of Bhi02G000332 vs. NCBI nr
Match: XP_038878610.1 (polygalacturonase 1 beta-like protein 3 [Benincasa hispida])

HSP 1 Score: 1234.2 bits (3192), Expect = 0.0e+00
Identity = 620/620 (100.00%), Postives = 620/620 (100.00%), Query Frame = 0

Query: 1   MVHIVFFLLLSQLSLSFFHVSFASADHQQSNPFTAKASLLRYWNSHVSNQLPPPDFLFSK 60
           MVHIVFFLLLSQLSLSFFHVSFASADHQQSNPFTAKASLLRYWNSHVSNQLPPPDFLFSK
Sbjct: 1   MVHIVFFLLLSQLSLSFFHVSFASADHQQSNPFTAKASLLRYWNSHVSNQLPPPDFLFSK 60

Query: 61  ASPLSAVDAAVLAQHAARGSLSFRLESFCYSANLFCSFDSETEISVPRLKDADFKLYGNK 120
           ASPLSAVDAAVLAQHAARGSLSFRLESFCYSANLFCSFDSETEISVPRLKDADFKLYGNK
Sbjct: 61  ASPLSAVDAAVLAQHAARGSLSFRLESFCYSANLFCSFDSETEISVPRLKDADFKLYGNK 120

Query: 121 NFVNYGGARLGGTDSFKNYSDGLNTADHSFKRYGGNSSNHEEGFTNYAEDGNVANSNFIS 180
           NFVNYGGARLGGTDSFKNYSDGLNTADHSFKRYGGNSSNHEEGFTNYAEDGNVANSNFIS
Sbjct: 121 NFVNYGGARLGGTDSFKNYSDGLNTADHSFKRYGGNSSNHEEGFTNYAEDGNVANSNFIS 180

Query: 181 YATESKGGSGEFVNYDKLVNVPNLRFTAYDLNSRNHKLSFTGYGNETNSGSQTFTSYGKK 240
           YATESKGGSGEFVNYDKLVNVPNLRFTAYDLNSRNHKLSFTGYGNETNSGSQTFTSYGKK
Sbjct: 181 YATESKGGSGEFVNYDKLVNVPNLRFTAYDLNSRNHKLSFTGYGNETNSGSQTFTSYGKK 240

Query: 241 GNGVPQEFSSYSDGSNILTSTFTGYGESGKTANDSFKGYGVSGNNPHTNFKNYGKGGNSE 300
           GNGVPQEFSSYSDGSNILTSTFTGYGESGKTANDSFKGYGVSGNNPHTNFKNYGKGGNSE
Sbjct: 241 GNGVPQEFSSYSDGSNILTSTFTGYGESGKTANDSFKGYGVSGNNPHTNFKNYGKGGNSE 300

Query: 301 IDTFSNYRNGANVGDDSFQSYAKNSNSGRVNFANYGKSFNHGNDSFTEYGKGSIGRTTVG 360
           IDTFSNYRNGANVGDDSFQSYAKNSNSGRVNFANYGKSFNHGNDSFTEYGKGSIGRTTVG
Sbjct: 301 IDTFSNYRNGANVGDDSFQSYAKNSNSGRVNFANYGKSFNHGNDSFTEYGKGSIGRTTVG 360

Query: 361 FKSYSVDRNFTTYAQKGVSFSEYSNFTTATGSGIHVNKLVEPGKFFRESELKQGNVMVMP 420
           FKSYSVDRNFTTYAQKGVSFSEYSNFTTATGSGIHVNKLVEPGKFFRESELKQGNVMVMP
Sbjct: 361 FKSYSVDRNFTTYAQKGVSFSEYSNFTTATGSGIHVNKLVEPGKFFRESELKQGNVMVMP 420

Query: 421 DIRDKMPPRSFLPRPILVNLPFSSSRISEAKEIFHARDGSTMERVITNALGECERAPSPG 480
           DIRDKMPPRSFLPRPILVNLPFSSSRISEAKEIFHARDGSTMERVITNALGECERAPSPG
Sbjct: 421 DIRDKMPPRSFLPRPILVNLPFSSSRISEAKEIFHARDGSTMERVITNALGECERAPSPG 480

Query: 481 ETKRCVGSAEDMIDFATTVLGRRVVARTTANVNGSREKVEIGTVKGINGGDLTSSVSCHQ 540
           ETKRCVGSAEDMIDFATTVLGRRVVARTTANVNGSREKVEIGTVKGINGGDLTSSVSCHQ
Sbjct: 481 ETKRCVGSAEDMIDFATTVLGRRVVARTTANVNGSREKVEIGTVKGINGGDLTSSVSCHQ 540

Query: 541 SLYPYLLYYCHSVPKVRIYEADILRLKTKEKINRGVAICHMDTSSWGPEHGAFVALGSGP 600
           SLYPYLLYYCHSVPKVRIYEADILRLKTKEKINRGVAICHMDTSSWGPEHGAFVALGSGP
Sbjct: 541 SLYPYLLYYCHSVPKVRIYEADILRLKTKEKINRGVAICHMDTSSWGPEHGAFVALGSGP 600

Query: 601 GQIEVCHWIFENDMSWSVAD 621
           GQIEVCHWIFENDMSWSVAD
Sbjct: 601 GQIEVCHWIFENDMSWSVAD 620

BLAST of Bhi02G000332 vs. NCBI nr
Match: XP_022921994.1 (polygalacturonase 1 beta-like protein 3 [Cucurbita moschata])

HSP 1 Score: 1087.0 bits (2810), Expect = 0.0e+00
Identity = 550/626 (87.86%), Postives = 581/626 (92.81%), Query Frame = 0

Query: 1   MVHIVFFLLLSQLSLSFFHVSFASADHQ------QSNPFTAKASLLRYWNSHVSNQLPPP 60
           MVHIVFFLLLSQLSLS  HVSF SAD        Q NPFTAKASL+RYWN+HVSNQLP P
Sbjct: 6   MVHIVFFLLLSQLSLSLLHVSFVSADQATFNASIQFNPFTAKASLVRYWNTHVSNQLPKP 65

Query: 61  DFLFSKASPLSAVDAAVLAQHAARGSLSFRLESFCYSANLFCSFDSETEISVPRLKDADF 120
           DFLF+KASPLSAVDAAV A+HAA GSL+ RLE+FCYSANLFCSFDS+ E SVPR KDADF
Sbjct: 66  DFLFAKASPLSAVDAAVFAKHAALGSLTSRLETFCYSANLFCSFDSQ-ETSVPRFKDADF 125

Query: 121 KLYGNKNFVNYGGARLGGTDSFKNYSDGLNTADHSFKRYGGNSSNHEEGFTNYAEDGNVA 180
           KLYGNKNF NYG AR+GG DSFKNYSDGLNTADHSFKRYGGNSSNH+EGFTNYAEDGNVA
Sbjct: 126 KLYGNKNFNNYGAARIGGADSFKNYSDGLNTADHSFKRYGGNSSNHKEGFTNYAEDGNVA 185

Query: 181 NSNFISYATESKGGSGEFVNYDKLVNVPNLRFTAYDLNSRNHKLSFTGYGNETNSGSQTF 240
           N+NFISYA ESKGGSGEFVNYDK+VNVPNLRFTAYDL+SR HKLSFT YGNETNSGSQ+F
Sbjct: 186 NNNFISYAAESKGGSGEFVNYDKIVNVPNLRFTAYDLDSRAHKLSFTSYGNETNSGSQSF 245

Query: 241 TSYGKKGNGVPQEFSSYSDGSNILTSTFTGYGESGKTANDSFKGYGVSGNNPHTNFKNYG 300
           TSYGK GNGVPQEFSSYSDGSNILTSTFTGYGESGK+ANDSFK YG+SGNNPH+NFKNYG
Sbjct: 246 TSYGKNGNGVPQEFSSYSDGSNILTSTFTGYGESGKSANDSFKAYGLSGNNPHSNFKNYG 305

Query: 301 KGGNSEIDTFSNYRNGANVGDDSFQSYAKNSNSGRVNFANYGKSFNHGNDSFTEYGKGSI 360
           KGG S+IDTFSNYRNGANVGDDSFQSYAKNSNSGRVNFA+YG+SFNHGNDSF EYGKGSI
Sbjct: 306 KGGKSQIDTFSNYRNGANVGDDSFQSYAKNSNSGRVNFASYGQSFNHGNDSFKEYGKGSI 365

Query: 361 GRTTVGFKSYSVDRNFTTYAQKGVSFSEYSNFTTATGSGIHVNKLVEPGKFFRESELKQG 420
           G+TTVGF+SYSVDRNFTTYAQKGVSFSEYS    ATGSGI+VN+ VEPGKFFRESELKQG
Sbjct: 366 GQTTVGFRSYSVDRNFTTYAQKGVSFSEYS----ATGSGINVNRWVEPGKFFRESELKQG 425

Query: 421 NVMVMPDIRDKMPPRSFLPRPILVNLPFSSSRISEAKEIFHARDGSTMERVITNALGECE 480
           NVMVMPDIRDKMPPRSFLPR ILVN+PFSSS+ISE K+IFHAR+GSTME VIT+AL ECE
Sbjct: 426 NVMVMPDIRDKMPPRSFLPRAILVNIPFSSSKISETKQIFHARNGSTMEHVITSALAECE 485

Query: 481 RAPSPGETKRCVGSAEDMIDFATTVLGRRVVARTTANVNGSREKVEIGTVKGINGGDLTS 540
           RAPS GETKRCVGSAEDMIDFAT VLGR VV RTTA+VNGS+EKVEIG VKGINGG+LT 
Sbjct: 486 RAPSMGETKRCVGSAEDMIDFATAVLGRNVVVRTTASVNGSKEKVEIGEVKGINGGELTR 545

Query: 541 SVSCHQSLYPYLLYYCHSVPKVRIYEADILRLKTKEKINRGVAICHMDTSSWGPEHGAFV 600
           SVSCHQSLYPYLLYYCHSVPKVRIYEADILRLKT EKINRGVAICHMDTSSWGPEHGAFV
Sbjct: 546 SVSCHQSLYPYLLYYCHSVPKVRIYEADILRLKTNEKINRGVAICHMDTSSWGPEHGAFV 605

Query: 601 ALGSGPGQIEVCHWIFENDMSWSVAD 621
           ALGSGPGQIEVCHWIFENDMSWSVAD
Sbjct: 606 ALGSGPGQIEVCHWIFENDMSWSVAD 626

BLAST of Bhi02G000332 vs. NCBI nr
Match: XP_008453099.1 (PREDICTED: polygalacturonase 1 beta-like protein 3 [Cucumis melo])

HSP 1 Score: 1085.9 bits (2807), Expect = 0.0e+00
Identity = 553/648 (85.34%), Postives = 579/648 (89.35%), Query Frame = 0

Query: 1   MVHIV----FFLLLSQLSLSFFHVS-------------------FAS-----ADHQQSNP 60
           MVHI     FF  +SQLSLSFFH++                   F S     +    SNP
Sbjct: 1   MVHIFSSSSFFFFISQLSLSFFHLTTNKQISFKVLFLFLLPFFFFLSFFPQISSAASSNP 60

Query: 61  FTAKASLLRYWNSHVSNQLPPPDFLFSKASPLSAVDAAVLAQHAARGSLSFRLESFCYSA 120
           FT KASL+RYW +HVS  LP PDF  SKASPLS+VDAAVLA HAA  SLS RLESFCYSA
Sbjct: 61  FTPKASLVRYWKTHVSTDLPTPDFFLSKASPLSSVDAAVLATHAAGASLSSRLESFCYSA 120

Query: 121 NLFCSFDSETEISVPRLKDADFKLYGNKNFVNYGGARLGGTDSFKNYSDGLNTADHSFKR 180
           NLFCSFDS++EISVPR KDADFKLYGNKNF NYGG RLGGTDSFKNYSDGLNTA+HSFKR
Sbjct: 121 NLFCSFDSQSEISVPRFKDADFKLYGNKNFANYGGNRLGGTDSFKNYSDGLNTAEHSFKR 180

Query: 181 YGGNSSNHEEGFTNYAEDGNVANSNFISYATESKGGSGEFVNYDKLVNVPNLRFTAYDLN 240
           YGGNSSNH+EGFT+YAEDGNVANSNF+SY  ESK GSG F NYDKLVNVPNLRFTAYDLN
Sbjct: 181 YGGNSSNHKEGFTSYAEDGNVANSNFVSYGAESKSGSGGFTNYDKLVNVPNLRFTAYDLN 240

Query: 241 SRNHKLSFTGYGNETNSGSQTFTSYGKKGNGVPQEFSSYSDGSNILTSTFTGYGESGKTA 300
           SR+HKL+FT YGNETNSGSQ+FTSYGK GNGVPQ+FSSYSDGSNILTSTFTGYGESGKTA
Sbjct: 241 SRSHKLTFTSYGNETNSGSQSFTSYGKNGNGVPQDFSSYSDGSNILTSTFTGYGESGKTA 300

Query: 301 NDSFKGYGVSGNNPHTNFKNYGKGGNSEIDTFSNYRNGANVGDDSFQSYAKNSNSGRVNF 360
           NDSFK YGVSGNNPHTNFKNYGKGGNSEIDTFSNYRNGANVGDDSFQSYAKNSNSGRVNF
Sbjct: 301 NDSFKEYGVSGNNPHTNFKNYGKGGNSEIDTFSNYRNGANVGDDSFQSYAKNSNSGRVNF 360

Query: 361 ANYGKSFNHGNDSFTEYGKGSIGRTTVGFKSYSVDRNFTTYAQKGVSFSEYSNFTTATGS 420
           ANYGKSFNHGNDSFTEYGKGSIG+TTVGFKSYS+DRNFTTYA+ GVSFSEYSNFTTATGS
Sbjct: 361 ANYGKSFNHGNDSFTEYGKGSIGKTTVGFKSYSIDRNFTTYAKNGVSFSEYSNFTTATGS 420

Query: 421 GIHVNKLVEPGKFFRESELKQGNVMVMPDIRDKMPPRSFLPRPILVNLPFSSSRISEAKE 480
           GIHVN  VEPGKFFRESELKQGNVMVMPDIRDKMPPRSFLPR ILVNLPFSSSRISEAKE
Sbjct: 421 GIHVNSHVEPGKFFRESELKQGNVMVMPDIRDKMPPRSFLPRAILVNLPFSSSRISEAKE 480

Query: 481 IFHARDGSTMERVITNALGECERAPSPGETKRCVGSAEDMIDFATTVLGRRVVARTTANV 540
           +FHARDGSTME VITNAL ECERAPS GETKRCVGSAEDMIDFATTVLGRRVV RTTANV
Sbjct: 481 MFHARDGSTMESVITNALAECERAPSRGETKRCVGSAEDMIDFATTVLGRRVVVRTTANV 540

Query: 541 NGSREKVEIGTVKGINGGDLTSSVSCHQSLYPYLLYYCHSVPKVRIYEADILRLKTKEKI 600
           NGS+EKVEIG VKGINGG+LT SVSCHQSLYPYLLYYCHSVPKVRIYEADILR++TKEKI
Sbjct: 541 NGSKEKVEIGRVKGINGGNLTRSVSCHQSLYPYLLYYCHSVPKVRIYEADILRVETKEKI 600

Query: 601 NRGVAICHMDTSSWGPEHGAFVALGSGPGQIEVCHWIFENDMSWSVAD 621
           NRGVAICH+DTSSWGPEHGAFVALGS PGQIEVCHWIFENDMSWSVAD
Sbjct: 601 NRGVAICHIDTSSWGPEHGAFVALGSSPGQIEVCHWIFENDMSWSVAD 648

BLAST of Bhi02G000332 vs. NCBI nr
Match: KAG6589452.1 (Polygalacturonase 1 beta-like protein 3, partial [Cucurbita argyrosperma subsp. sororia] >KAG7023135.1 Polygalacturonase 1 beta-like protein 3 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 1084.7 bits (2804), Expect = 0.0e+00
Identity = 549/626 (87.70%), Postives = 580/626 (92.65%), Query Frame = 0

Query: 1   MVHIVFFLLLSQLSLSFFHVSFASADHQ------QSNPFTAKASLLRYWNSHVSNQLPPP 60
           MVHIVFFLLLSQLSLS  HVSF SAD        Q NPFTAKASL+RYWN+HVSNQLP P
Sbjct: 6   MVHIVFFLLLSQLSLSLLHVSFVSADQATFNASIQFNPFTAKASLVRYWNTHVSNQLPKP 65

Query: 61  DFLFSKASPLSAVDAAVLAQHAARGSLSFRLESFCYSANLFCSFDSETEISVPRLKDADF 120
           DFLF+KASPLSAVDAAV A+HAA GSL+ RLE+FCYSANLFCSFDS+ E S+PR KDADF
Sbjct: 66  DFLFAKASPLSAVDAAVFAKHAALGSLTSRLETFCYSANLFCSFDSQ-ETSIPRFKDADF 125

Query: 121 KLYGNKNFVNYGGARLGGTDSFKNYSDGLNTADHSFKRYGGNSSNHEEGFTNYAEDGNVA 180
           KLYGNKNF NYG AR+GG DSFKNYSDGLNTADHSFKRYGGNSSNH+EGF NYAEDGNVA
Sbjct: 126 KLYGNKNFNNYGAARIGGADSFKNYSDGLNTADHSFKRYGGNSSNHKEGFMNYAEDGNVA 185

Query: 181 NSNFISYATESKGGSGEFVNYDKLVNVPNLRFTAYDLNSRNHKLSFTGYGNETNSGSQTF 240
           N+NFISYA ESKGGSGEFVNYDK+VNVPNLRFTAYDL+SR HKLSFT YGNETNSGSQ+F
Sbjct: 186 NNNFISYAAESKGGSGEFVNYDKIVNVPNLRFTAYDLDSRAHKLSFTSYGNETNSGSQSF 245

Query: 241 TSYGKKGNGVPQEFSSYSDGSNILTSTFTGYGESGKTANDSFKGYGVSGNNPHTNFKNYG 300
           TSYGK GNGVPQEFSSYSDGSNILTSTFTGYGESGK+ANDSFK YG+SGNNPH+NFKNYG
Sbjct: 246 TSYGKNGNGVPQEFSSYSDGSNILTSTFTGYGESGKSANDSFKAYGLSGNNPHSNFKNYG 305

Query: 301 KGGNSEIDTFSNYRNGANVGDDSFQSYAKNSNSGRVNFANYGKSFNHGNDSFTEYGKGSI 360
           KGG S+IDTFSNYRNGANVGDDSFQSYAKNSNSGRVNFA+YG+SFNHGNDSF EYGKGSI
Sbjct: 306 KGGKSQIDTFSNYRNGANVGDDSFQSYAKNSNSGRVNFASYGQSFNHGNDSFKEYGKGSI 365

Query: 361 GRTTVGFKSYSVDRNFTTYAQKGVSFSEYSNFTTATGSGIHVNKLVEPGKFFRESELKQG 420
           G+TTVGF+SYSVDRNFTTYAQKGVSFSEYS    ATGSGI+VN+ VEPGKFFRESELKQG
Sbjct: 366 GQTTVGFRSYSVDRNFTTYAQKGVSFSEYS----ATGSGINVNRWVEPGKFFRESELKQG 425

Query: 421 NVMVMPDIRDKMPPRSFLPRPILVNLPFSSSRISEAKEIFHARDGSTMERVITNALGECE 480
           NVMVMPDIRDKMPPRSFLPR ILVN+PFSSS+ISE K+IFHAR+GSTME VIT+AL ECE
Sbjct: 426 NVMVMPDIRDKMPPRSFLPRAILVNIPFSSSKISETKQIFHARNGSTMEHVITSALAECE 485

Query: 481 RAPSPGETKRCVGSAEDMIDFATTVLGRRVVARTTANVNGSREKVEIGTVKGINGGDLTS 540
           RAPS GETKRCVGSAEDMIDFAT VLGR VVARTTA+VNGS EKVEIG VKGINGG+LT 
Sbjct: 486 RAPSMGETKRCVGSAEDMIDFATAVLGRNVVARTTASVNGSTEKVEIGEVKGINGGELTR 545

Query: 541 SVSCHQSLYPYLLYYCHSVPKVRIYEADILRLKTKEKINRGVAICHMDTSSWGPEHGAFV 600
           SVSCHQSLYPYLLYYCHSVPKVRIYEADILRLKT EKINRGVAICHMDTSSWGPEHGAFV
Sbjct: 546 SVSCHQSLYPYLLYYCHSVPKVRIYEADILRLKTNEKINRGVAICHMDTSSWGPEHGAFV 605

Query: 601 ALGSGPGQIEVCHWIFENDMSWSVAD 621
           ALGSGPGQIEVCHWIFENDMSWSVAD
Sbjct: 606 ALGSGPGQIEVCHWIFENDMSWSVAD 626

BLAST of Bhi02G000332 vs. NCBI nr
Match: XP_004138283.1 (polygalacturonase 1 beta-like protein 3 [Cucumis sativus] >KGN63588.1 hypothetical protein Csa_013467 [Cucumis sativus])

HSP 1 Score: 1081.2 bits (2795), Expect = 0.0e+00
Identity = 546/645 (84.65%), Postives = 577/645 (89.46%), Query Frame = 0

Query: 1   MVHIVFFLL--LSQLSLSFFHVS-----------------------FASADHQQSNPFTA 60
           MVHI  FL+  LSQLSLSFFH++                       F       SNPFT 
Sbjct: 1   MVHIFSFLIFFLSQLSLSFFHLTTNKQISFKFLFLFLLPFIFFLSFFPQISSASSNPFTP 60

Query: 61  KASLLRYWNSHVSNQLPPPDFLFSKASPLSAVDAAVLAQHAARGSLSFRLESFCYSANLF 120
           KASL+RYW +HVS  LP PDF  SKASPLSAVDAA LA HAA  SLS RLESFC+SANLF
Sbjct: 61  KASLVRYWKTHVSTDLPTPDFFLSKASPLSAVDAAALATHAAGASLSSRLESFCFSANLF 120

Query: 121 CSFDSETEISVPRLKDADFKLYGNKNFVNYGGARLGGTDSFKNYSDGLNTADHSFKRYGG 180
           CSFDS++E SVPR KDADFKLYGNKNFVNYGG+RLGGTDSFKNYSDGLNTA+HSFKRYGG
Sbjct: 121 CSFDSQSETSVPRSKDADFKLYGNKNFVNYGGSRLGGTDSFKNYSDGLNTAEHSFKRYGG 180

Query: 181 NSSNHEEGFTNYAEDGNVANSNFISYATESKGGSGEFVNYDKLVNVPNLRFTAYDLNSRN 240
           NSSNH+EGFT+YAEDGNVANSNF+SY   SK GSGEF NYDKLVNVPNLRFTAYDLNSR+
Sbjct: 181 NSSNHKEGFTSYAEDGNVANSNFVSYGAGSKSGSGEFTNYDKLVNVPNLRFTAYDLNSRS 240

Query: 241 HKLSFTGYGNETNSGSQTFTSYGKKGNGVPQEFSSYSDGSNILTSTFTGYGESGKTANDS 300
           HKLSFT YGNETNSGSQ+FTSYGK GNGVPQ+FSSYSDGSNILTSTFTGYGESGKTANDS
Sbjct: 241 HKLSFTSYGNETNSGSQSFTSYGKNGNGVPQDFSSYSDGSNILTSTFTGYGESGKTANDS 300

Query: 301 FKGYGVSGNNPHTNFKNYGKGGNSEIDTFSNYRNGANVGDDSFQSYAKNSNSGRVNFANY 360
           FK YG+SGNNPHTNFKNYGKGGNSEIDTFSNYRNGANVGDDSFQSYAKNSNSGRVNFANY
Sbjct: 301 FKEYGISGNNPHTNFKNYGKGGNSEIDTFSNYRNGANVGDDSFQSYAKNSNSGRVNFANY 360

Query: 361 GKSFNHGNDSFTEYGKGSIGRTTVGFKSYSVDRNFTTYAQKGVSFSEYSNFTTATGSGIH 420
           GKSFNHGNDSFTEYGKGS+G+TTVGFKSYSVDRNFTTYA+ GVSFS Y+NFTTATGSGIH
Sbjct: 361 GKSFNHGNDSFTEYGKGSMGKTTVGFKSYSVDRNFTTYAKNGVSFSVYNNFTTATGSGIH 420

Query: 421 VNKLVEPGKFFRESELKQGNVMVMPDIRDKMPPRSFLPRPILVNLPFSSSRISEAKEIFH 480
            N  VEPGKFFRESELKQGNVMVMPDI+DKMPPRSFLPR ILVNLPFSSSRISEAKE+FH
Sbjct: 421 ANSHVEPGKFFRESELKQGNVMVMPDIKDKMPPRSFLPRSILVNLPFSSSRISEAKEMFH 480

Query: 481 ARDGSTMERVITNALGECERAPSPGETKRCVGSAEDMIDFATTVLGRRVVARTTANVNGS 540
           ARDGSTME VITNAL ECERAPS GETKRCVGSAEDMIDFATTVLG+RVV RTTANVNGS
Sbjct: 481 ARDGSTMESVITNALAECERAPSRGETKRCVGSAEDMIDFATTVLGQRVVVRTTANVNGS 540

Query: 541 REKVEIGTVKGINGGDLTSSVSCHQSLYPYLLYYCHSVPKVRIYEADILRLKTKEKINRG 600
           +EK+EIG VKGINGGDLT SVSCHQSLYPYLLYYCHSVPKVRIYEADIL+++TKEKINRG
Sbjct: 541 KEKIEIGRVKGINGGDLTRSVSCHQSLYPYLLYYCHSVPKVRIYEADILKMETKEKINRG 600

Query: 601 VAICHMDTSSWGPEHGAFVALGSGPGQIEVCHWIFENDMSWSVAD 621
           VAICH+DTSSWGPEHGAFVALGSGPGQIEVCHWIFENDMSW+VAD
Sbjct: 601 VAICHIDTSSWGPEHGAFVALGSGPGQIEVCHWIFENDMSWNVAD 645

BLAST of Bhi02G000332 vs. ExPASy TrEMBL
Match: A0A6J1E242 (polygalacturonase 1 beta-like protein 3 OS=Cucurbita moschata OX=3662 GN=LOC111430079 PE=4 SV=1)

HSP 1 Score: 1087.0 bits (2810), Expect = 0.0e+00
Identity = 550/626 (87.86%), Postives = 581/626 (92.81%), Query Frame = 0

Query: 1   MVHIVFFLLLSQLSLSFFHVSFASADHQ------QSNPFTAKASLLRYWNSHVSNQLPPP 60
           MVHIVFFLLLSQLSLS  HVSF SAD        Q NPFTAKASL+RYWN+HVSNQLP P
Sbjct: 6   MVHIVFFLLLSQLSLSLLHVSFVSADQATFNASIQFNPFTAKASLVRYWNTHVSNQLPKP 65

Query: 61  DFLFSKASPLSAVDAAVLAQHAARGSLSFRLESFCYSANLFCSFDSETEISVPRLKDADF 120
           DFLF+KASPLSAVDAAV A+HAA GSL+ RLE+FCYSANLFCSFDS+ E SVPR KDADF
Sbjct: 66  DFLFAKASPLSAVDAAVFAKHAALGSLTSRLETFCYSANLFCSFDSQ-ETSVPRFKDADF 125

Query: 121 KLYGNKNFVNYGGARLGGTDSFKNYSDGLNTADHSFKRYGGNSSNHEEGFTNYAEDGNVA 180
           KLYGNKNF NYG AR+GG DSFKNYSDGLNTADHSFKRYGGNSSNH+EGFTNYAEDGNVA
Sbjct: 126 KLYGNKNFNNYGAARIGGADSFKNYSDGLNTADHSFKRYGGNSSNHKEGFTNYAEDGNVA 185

Query: 181 NSNFISYATESKGGSGEFVNYDKLVNVPNLRFTAYDLNSRNHKLSFTGYGNETNSGSQTF 240
           N+NFISYA ESKGGSGEFVNYDK+VNVPNLRFTAYDL+SR HKLSFT YGNETNSGSQ+F
Sbjct: 186 NNNFISYAAESKGGSGEFVNYDKIVNVPNLRFTAYDLDSRAHKLSFTSYGNETNSGSQSF 245

Query: 241 TSYGKKGNGVPQEFSSYSDGSNILTSTFTGYGESGKTANDSFKGYGVSGNNPHTNFKNYG 300
           TSYGK GNGVPQEFSSYSDGSNILTSTFTGYGESGK+ANDSFK YG+SGNNPH+NFKNYG
Sbjct: 246 TSYGKNGNGVPQEFSSYSDGSNILTSTFTGYGESGKSANDSFKAYGLSGNNPHSNFKNYG 305

Query: 301 KGGNSEIDTFSNYRNGANVGDDSFQSYAKNSNSGRVNFANYGKSFNHGNDSFTEYGKGSI 360
           KGG S+IDTFSNYRNGANVGDDSFQSYAKNSNSGRVNFA+YG+SFNHGNDSF EYGKGSI
Sbjct: 306 KGGKSQIDTFSNYRNGANVGDDSFQSYAKNSNSGRVNFASYGQSFNHGNDSFKEYGKGSI 365

Query: 361 GRTTVGFKSYSVDRNFTTYAQKGVSFSEYSNFTTATGSGIHVNKLVEPGKFFRESELKQG 420
           G+TTVGF+SYSVDRNFTTYAQKGVSFSEYS    ATGSGI+VN+ VEPGKFFRESELKQG
Sbjct: 366 GQTTVGFRSYSVDRNFTTYAQKGVSFSEYS----ATGSGINVNRWVEPGKFFRESELKQG 425

Query: 421 NVMVMPDIRDKMPPRSFLPRPILVNLPFSSSRISEAKEIFHARDGSTMERVITNALGECE 480
           NVMVMPDIRDKMPPRSFLPR ILVN+PFSSS+ISE K+IFHAR+GSTME VIT+AL ECE
Sbjct: 426 NVMVMPDIRDKMPPRSFLPRAILVNIPFSSSKISETKQIFHARNGSTMEHVITSALAECE 485

Query: 481 RAPSPGETKRCVGSAEDMIDFATTVLGRRVVARTTANVNGSREKVEIGTVKGINGGDLTS 540
           RAPS GETKRCVGSAEDMIDFAT VLGR VV RTTA+VNGS+EKVEIG VKGINGG+LT 
Sbjct: 486 RAPSMGETKRCVGSAEDMIDFATAVLGRNVVVRTTASVNGSKEKVEIGEVKGINGGELTR 545

Query: 541 SVSCHQSLYPYLLYYCHSVPKVRIYEADILRLKTKEKINRGVAICHMDTSSWGPEHGAFV 600
           SVSCHQSLYPYLLYYCHSVPKVRIYEADILRLKT EKINRGVAICHMDTSSWGPEHGAFV
Sbjct: 546 SVSCHQSLYPYLLYYCHSVPKVRIYEADILRLKTNEKINRGVAICHMDTSSWGPEHGAFV 605

Query: 601 ALGSGPGQIEVCHWIFENDMSWSVAD 621
           ALGSGPGQIEVCHWIFENDMSWSVAD
Sbjct: 606 ALGSGPGQIEVCHWIFENDMSWSVAD 626

BLAST of Bhi02G000332 vs. ExPASy TrEMBL
Match: A0A1S3BVD2 (polygalacturonase 1 beta-like protein 3 OS=Cucumis melo OX=3656 GN=LOC103493915 PE=4 SV=1)

HSP 1 Score: 1085.9 bits (2807), Expect = 0.0e+00
Identity = 553/648 (85.34%), Postives = 579/648 (89.35%), Query Frame = 0

Query: 1   MVHIV----FFLLLSQLSLSFFHVS-------------------FAS-----ADHQQSNP 60
           MVHI     FF  +SQLSLSFFH++                   F S     +    SNP
Sbjct: 1   MVHIFSSSSFFFFISQLSLSFFHLTTNKQISFKVLFLFLLPFFFFLSFFPQISSAASSNP 60

Query: 61  FTAKASLLRYWNSHVSNQLPPPDFLFSKASPLSAVDAAVLAQHAARGSLSFRLESFCYSA 120
           FT KASL+RYW +HVS  LP PDF  SKASPLS+VDAAVLA HAA  SLS RLESFCYSA
Sbjct: 61  FTPKASLVRYWKTHVSTDLPTPDFFLSKASPLSSVDAAVLATHAAGASLSSRLESFCYSA 120

Query: 121 NLFCSFDSETEISVPRLKDADFKLYGNKNFVNYGGARLGGTDSFKNYSDGLNTADHSFKR 180
           NLFCSFDS++EISVPR KDADFKLYGNKNF NYGG RLGGTDSFKNYSDGLNTA+HSFKR
Sbjct: 121 NLFCSFDSQSEISVPRFKDADFKLYGNKNFANYGGNRLGGTDSFKNYSDGLNTAEHSFKR 180

Query: 181 YGGNSSNHEEGFTNYAEDGNVANSNFISYATESKGGSGEFVNYDKLVNVPNLRFTAYDLN 240
           YGGNSSNH+EGFT+YAEDGNVANSNF+SY  ESK GSG F NYDKLVNVPNLRFTAYDLN
Sbjct: 181 YGGNSSNHKEGFTSYAEDGNVANSNFVSYGAESKSGSGGFTNYDKLVNVPNLRFTAYDLN 240

Query: 241 SRNHKLSFTGYGNETNSGSQTFTSYGKKGNGVPQEFSSYSDGSNILTSTFTGYGESGKTA 300
           SR+HKL+FT YGNETNSGSQ+FTSYGK GNGVPQ+FSSYSDGSNILTSTFTGYGESGKTA
Sbjct: 241 SRSHKLTFTSYGNETNSGSQSFTSYGKNGNGVPQDFSSYSDGSNILTSTFTGYGESGKTA 300

Query: 301 NDSFKGYGVSGNNPHTNFKNYGKGGNSEIDTFSNYRNGANVGDDSFQSYAKNSNSGRVNF 360
           NDSFK YGVSGNNPHTNFKNYGKGGNSEIDTFSNYRNGANVGDDSFQSYAKNSNSGRVNF
Sbjct: 301 NDSFKEYGVSGNNPHTNFKNYGKGGNSEIDTFSNYRNGANVGDDSFQSYAKNSNSGRVNF 360

Query: 361 ANYGKSFNHGNDSFTEYGKGSIGRTTVGFKSYSVDRNFTTYAQKGVSFSEYSNFTTATGS 420
           ANYGKSFNHGNDSFTEYGKGSIG+TTVGFKSYS+DRNFTTYA+ GVSFSEYSNFTTATGS
Sbjct: 361 ANYGKSFNHGNDSFTEYGKGSIGKTTVGFKSYSIDRNFTTYAKNGVSFSEYSNFTTATGS 420

Query: 421 GIHVNKLVEPGKFFRESELKQGNVMVMPDIRDKMPPRSFLPRPILVNLPFSSSRISEAKE 480
           GIHVN  VEPGKFFRESELKQGNVMVMPDIRDKMPPRSFLPR ILVNLPFSSSRISEAKE
Sbjct: 421 GIHVNSHVEPGKFFRESELKQGNVMVMPDIRDKMPPRSFLPRAILVNLPFSSSRISEAKE 480

Query: 481 IFHARDGSTMERVITNALGECERAPSPGETKRCVGSAEDMIDFATTVLGRRVVARTTANV 540
           +FHARDGSTME VITNAL ECERAPS GETKRCVGSAEDMIDFATTVLGRRVV RTTANV
Sbjct: 481 MFHARDGSTMESVITNALAECERAPSRGETKRCVGSAEDMIDFATTVLGRRVVVRTTANV 540

Query: 541 NGSREKVEIGTVKGINGGDLTSSVSCHQSLYPYLLYYCHSVPKVRIYEADILRLKTKEKI 600
           NGS+EKVEIG VKGINGG+LT SVSCHQSLYPYLLYYCHSVPKVRIYEADILR++TKEKI
Sbjct: 541 NGSKEKVEIGRVKGINGGNLTRSVSCHQSLYPYLLYYCHSVPKVRIYEADILRVETKEKI 600

Query: 601 NRGVAICHMDTSSWGPEHGAFVALGSGPGQIEVCHWIFENDMSWSVAD 621
           NRGVAICH+DTSSWGPEHGAFVALGS PGQIEVCHWIFENDMSWSVAD
Sbjct: 601 NRGVAICHIDTSSWGPEHGAFVALGSSPGQIEVCHWIFENDMSWSVAD 648

BLAST of Bhi02G000332 vs. ExPASy TrEMBL
Match: A0A0A0LP63 (Polygalacturonase-1 non-catalytic subunit beta OS=Cucumis sativus OX=3659 GN=Csa_1G005490 PE=4 SV=1)

HSP 1 Score: 1081.2 bits (2795), Expect = 0.0e+00
Identity = 546/645 (84.65%), Postives = 577/645 (89.46%), Query Frame = 0

Query: 1   MVHIVFFLL--LSQLSLSFFHVS-----------------------FASADHQQSNPFTA 60
           MVHI  FL+  LSQLSLSFFH++                       F       SNPFT 
Sbjct: 1   MVHIFSFLIFFLSQLSLSFFHLTTNKQISFKFLFLFLLPFIFFLSFFPQISSASSNPFTP 60

Query: 61  KASLLRYWNSHVSNQLPPPDFLFSKASPLSAVDAAVLAQHAARGSLSFRLESFCYSANLF 120
           KASL+RYW +HVS  LP PDF  SKASPLSAVDAA LA HAA  SLS RLESFC+SANLF
Sbjct: 61  KASLVRYWKTHVSTDLPTPDFFLSKASPLSAVDAAALATHAAGASLSSRLESFCFSANLF 120

Query: 121 CSFDSETEISVPRLKDADFKLYGNKNFVNYGGARLGGTDSFKNYSDGLNTADHSFKRYGG 180
           CSFDS++E SVPR KDADFKLYGNKNFVNYGG+RLGGTDSFKNYSDGLNTA+HSFKRYGG
Sbjct: 121 CSFDSQSETSVPRSKDADFKLYGNKNFVNYGGSRLGGTDSFKNYSDGLNTAEHSFKRYGG 180

Query: 181 NSSNHEEGFTNYAEDGNVANSNFISYATESKGGSGEFVNYDKLVNVPNLRFTAYDLNSRN 240
           NSSNH+EGFT+YAEDGNVANSNF+SY   SK GSGEF NYDKLVNVPNLRFTAYDLNSR+
Sbjct: 181 NSSNHKEGFTSYAEDGNVANSNFVSYGAGSKSGSGEFTNYDKLVNVPNLRFTAYDLNSRS 240

Query: 241 HKLSFTGYGNETNSGSQTFTSYGKKGNGVPQEFSSYSDGSNILTSTFTGYGESGKTANDS 300
           HKLSFT YGNETNSGSQ+FTSYGK GNGVPQ+FSSYSDGSNILTSTFTGYGESGKTANDS
Sbjct: 241 HKLSFTSYGNETNSGSQSFTSYGKNGNGVPQDFSSYSDGSNILTSTFTGYGESGKTANDS 300

Query: 301 FKGYGVSGNNPHTNFKNYGKGGNSEIDTFSNYRNGANVGDDSFQSYAKNSNSGRVNFANY 360
           FK YG+SGNNPHTNFKNYGKGGNSEIDTFSNYRNGANVGDDSFQSYAKNSNSGRVNFANY
Sbjct: 301 FKEYGISGNNPHTNFKNYGKGGNSEIDTFSNYRNGANVGDDSFQSYAKNSNSGRVNFANY 360

Query: 361 GKSFNHGNDSFTEYGKGSIGRTTVGFKSYSVDRNFTTYAQKGVSFSEYSNFTTATGSGIH 420
           GKSFNHGNDSFTEYGKGS+G+TTVGFKSYSVDRNFTTYA+ GVSFS Y+NFTTATGSGIH
Sbjct: 361 GKSFNHGNDSFTEYGKGSMGKTTVGFKSYSVDRNFTTYAKNGVSFSVYNNFTTATGSGIH 420

Query: 421 VNKLVEPGKFFRESELKQGNVMVMPDIRDKMPPRSFLPRPILVNLPFSSSRISEAKEIFH 480
            N  VEPGKFFRESELKQGNVMVMPDI+DKMPPRSFLPR ILVNLPFSSSRISEAKE+FH
Sbjct: 421 ANSHVEPGKFFRESELKQGNVMVMPDIKDKMPPRSFLPRSILVNLPFSSSRISEAKEMFH 480

Query: 481 ARDGSTMERVITNALGECERAPSPGETKRCVGSAEDMIDFATTVLGRRVVARTTANVNGS 540
           ARDGSTME VITNAL ECERAPS GETKRCVGSAEDMIDFATTVLG+RVV RTTANVNGS
Sbjct: 481 ARDGSTMESVITNALAECERAPSRGETKRCVGSAEDMIDFATTVLGQRVVVRTTANVNGS 540

Query: 541 REKVEIGTVKGINGGDLTSSVSCHQSLYPYLLYYCHSVPKVRIYEADILRLKTKEKINRG 600
           +EK+EIG VKGINGGDLT SVSCHQSLYPYLLYYCHSVPKVRIYEADIL+++TKEKINRG
Sbjct: 541 KEKIEIGRVKGINGGDLTRSVSCHQSLYPYLLYYCHSVPKVRIYEADILKMETKEKINRG 600

Query: 601 VAICHMDTSSWGPEHGAFVALGSGPGQIEVCHWIFENDMSWSVAD 621
           VAICH+DTSSWGPEHGAFVALGSGPGQIEVCHWIFENDMSW+VAD
Sbjct: 601 VAICHIDTSSWGPEHGAFVALGSGPGQIEVCHWIFENDMSWNVAD 645

BLAST of Bhi02G000332 vs. ExPASy TrEMBL
Match: A0A5D3BIQ0 (Polygalacturonase 1 beta-like protein 3 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold350G001790 PE=4 SV=1)

HSP 1 Score: 1080.1 bits (2792), Expect = 0.0e+00
Identity = 536/591 (90.69%), Postives = 558/591 (94.42%), Query Frame = 0

Query: 30  SNPFTAKASLLRYWNSHVSNQLPPPDFLFSKASPLSAVDAAVLAQHAARGSLSFRLESFC 89
           SNPFT KASL+RYW +HVS  LP PDF  SKASPLS+VDAAVLA HAA  SLS RLESFC
Sbjct: 8   SNPFTPKASLVRYWKTHVSTDLPTPDFFLSKASPLSSVDAAVLATHAAGASLSSRLESFC 67

Query: 90  YSANLFCSFDSETEISVPRLKDADFKLYGNKNFVNYGGARLGGTDSFKNYSDGLNTADHS 149
           YSANLFCSFDS++EISVPR KDADFKLYGNKNF NYGG RLGGTDSFKNYSDGLNTA+HS
Sbjct: 68  YSANLFCSFDSQSEISVPRFKDADFKLYGNKNFANYGGNRLGGTDSFKNYSDGLNTAEHS 127

Query: 150 FKRYGGNSSNHEEGFTNYAEDGNVANSNFISYATESKGGSGEFVNYDKLVNVPNLRFTAY 209
           FKRYGGNSSNH+EGFT+YAEDGNVANSNF+SY  ESK GSG F NYDKLVNVPNLRFTAY
Sbjct: 128 FKRYGGNSSNHKEGFTSYAEDGNVANSNFVSYGAESKSGSGGFTNYDKLVNVPNLRFTAY 187

Query: 210 DLNSRNHKLSFTGYGNETNSGSQTFTSYGKKGNGVPQEFSSYSDGSNILTSTFTGYGESG 269
           DLNSR+HKL+FT YGNETNSGSQ+FTSYGK GNGVPQ+FSSYSDGSNILTSTFTGYGESG
Sbjct: 188 DLNSRSHKLTFTSYGNETNSGSQSFTSYGKNGNGVPQDFSSYSDGSNILTSTFTGYGESG 247

Query: 270 KTANDSFKGYGVSGNNPHTNFKNYGKGGNSEIDTFSNYRNGANVGDDSFQSYAKNSNSGR 329
           KTANDSFK YGVSGNNPHTNFKNYGKGGNSEIDTFSNYRNGANVGDDSFQSYAKNSNSGR
Sbjct: 248 KTANDSFKEYGVSGNNPHTNFKNYGKGGNSEIDTFSNYRNGANVGDDSFQSYAKNSNSGR 307

Query: 330 VNFANYGKSFNHGNDSFTEYGKGSIGRTTVGFKSYSVDRNFTTYAQKGVSFSEYSNFTTA 389
           VNFANYGKSFNHGNDSFTEYGKGSIG+TTVGFKSYS+DRNFTTYA+ GVSFSEYSNFTTA
Sbjct: 308 VNFANYGKSFNHGNDSFTEYGKGSIGKTTVGFKSYSIDRNFTTYAKNGVSFSEYSNFTTA 367

Query: 390 TGSGIHVNKLVEPGKFFRESELKQGNVMVMPDIRDKMPPRSFLPRPILVNLPFSSSRISE 449
           TGSGIHVN  VEPGKFFRESELKQGNVMVMPDIRDKMPPRSFLPR ILVNLPFSSSRISE
Sbjct: 368 TGSGIHVNSHVEPGKFFRESELKQGNVMVMPDIRDKMPPRSFLPRAILVNLPFSSSRISE 427

Query: 450 AKEIFHARDGSTMERVITNALGECERAPSPGETKRCVGSAEDMIDFATTVLGRRVVARTT 509
           AKE+FHARDGSTME VITNAL ECERAPS GETKRCVGSAEDMIDFATTVLGRRVV RTT
Sbjct: 428 AKEMFHARDGSTMESVITNALAECERAPSRGETKRCVGSAEDMIDFATTVLGRRVVVRTT 487

Query: 510 ANVNGSREKVEIGTVKGINGGDLTSSVSCHQSLYPYLLYYCHSVPKVRIYEADILRLKTK 569
           ANVNGS+EKVEIG VKGINGG+LT SVSCHQSLYPYLLYYCHSVPKVRIYEADILR++TK
Sbjct: 488 ANVNGSKEKVEIGRVKGINGGNLTRSVSCHQSLYPYLLYYCHSVPKVRIYEADILRVETK 547

Query: 570 EKINRGVAICHMDTSSWGPEHGAFVALGSGPGQIEVCHWIFENDMSWSVAD 621
           EKINRGVAICH+DTSSWGPEHGAFVALGS PGQIEVCHWIFENDMSWSVAD
Sbjct: 548 EKINRGVAICHIDTSSWGPEHGAFVALGSSPGQIEVCHWIFENDMSWSVAD 598

BLAST of Bhi02G000332 vs. ExPASy TrEMBL
Match: A0A6J1JM21 (polygalacturonase 1 beta-like protein 3 OS=Cucurbita maxima OX=3661 GN=LOC111485620 PE=4 SV=1)

HSP 1 Score: 1080.1 bits (2792), Expect = 0.0e+00
Identity = 548/626 (87.54%), Postives = 578/626 (92.33%), Query Frame = 0

Query: 1   MVHIVFFLLLSQLSLSFFHVSFASADHQ------QSNPFTAKASLLRYWNSHVSNQLPPP 60
           MVHIVFFLLLSQLSLS  HVSF SAD          NPFTAKASL+RYWN+HVSNQLP P
Sbjct: 6   MVHIVFFLLLSQLSLSLLHVSFVSADQATFNASIHFNPFTAKASLVRYWNTHVSNQLPKP 65

Query: 61  DFLFSKASPLSAVDAAVLAQHAARGSLSFRLESFCYSANLFCSFDSETEISVPRLKDADF 120
           DFLF KASPLSAVDAAV A+HAA GSL+ RLE+FCYSANLFCSFDS+ E SVPR KDADF
Sbjct: 66  DFLFVKASPLSAVDAAVFAKHAALGSLTSRLETFCYSANLFCSFDSQ-ETSVPRFKDADF 125

Query: 121 KLYGNKNFVNYGGARLGGTDSFKNYSDGLNTADHSFKRYGGNSSNHEEGFTNYAEDGNVA 180
           KLYGNKNF NYG AR+GG DSFKNYSDGLNTADHSFKRYGGNSSNH+EGFTNYAEDGNVA
Sbjct: 126 KLYGNKNFNNYGAARIGGADSFKNYSDGLNTADHSFKRYGGNSSNHKEGFTNYAEDGNVA 185

Query: 181 NSNFISYATESKGGSGEFVNYDKLVNVPNLRFTAYDLNSRNHKLSFTGYGNETNSGSQTF 240
           N+NFISYA ESKGGSGEFVNYDK+VNVPNLRFTAYDL+SR HKLSFT YGNETNSGSQ+F
Sbjct: 186 NNNFISYAAESKGGSGEFVNYDKIVNVPNLRFTAYDLDSRAHKLSFTSYGNETNSGSQSF 245

Query: 241 TSYGKKGNGVPQEFSSYSDGSNILTSTFTGYGESGKTANDSFKGYGVSGNNPHTNFKNYG 300
           TSYGK GNGVPQEFSSYSDGSNILTSTFTGYGESGK+ANDSFK YG+S NNPH+NFKNYG
Sbjct: 246 TSYGKNGNGVPQEFSSYSDGSNILTSTFTGYGESGKSANDSFKAYGLSSNNPHSNFKNYG 305

Query: 301 KGGNSEIDTFSNYRNGANVGDDSFQSYAKNSNSGRVNFANYGKSFNHGNDSFTEYGKGSI 360
           KGG S+IDTFSNYRNGANVGDDSFQSYAKNSNSGRVNFA+YG+SFNHGNDSF EY KGSI
Sbjct: 306 KGGKSQIDTFSNYRNGANVGDDSFQSYAKNSNSGRVNFASYGQSFNHGNDSFKEYAKGSI 365

Query: 361 GRTTVGFKSYSVDRNFTTYAQKGVSFSEYSNFTTATGSGIHVNKLVEPGKFFRESELKQG 420
           G+TTVGF+SYSVDRNFTTYAQKGVSFSEYS    ATGSGI+VN+ VEPGKFFRESELKQG
Sbjct: 366 GQTTVGFRSYSVDRNFTTYAQKGVSFSEYS----ATGSGINVNRWVEPGKFFRESELKQG 425

Query: 421 NVMVMPDIRDKMPPRSFLPRPILVNLPFSSSRISEAKEIFHARDGSTMERVITNALGECE 480
           NVMVMPDIRDKMPPRSFLPR ILVN+PFSSS+ISE K+IFHAR+GSTMERVIT+AL ECE
Sbjct: 426 NVMVMPDIRDKMPPRSFLPRAILVNIPFSSSKISETKQIFHARNGSTMERVITSALAECE 485

Query: 481 RAPSPGETKRCVGSAEDMIDFATTVLGRRVVARTTANVNGSREKVEIGTVKGINGGDLTS 540
           RAPS GETKRCVGSAEDMIDFAT VLGR VVARTTA+VNGS EKVEIG VKGINGG+LT 
Sbjct: 486 RAPSMGETKRCVGSAEDMIDFATAVLGRNVVARTTASVNGSTEKVEIGEVKGINGGELTR 545

Query: 541 SVSCHQSLYPYLLYYCHSVPKVRIYEADILRLKTKEKINRGVAICHMDTSSWGPEHGAFV 600
           SVSCHQSLYPYLLYYCHSVPKVRIYEADILRLKT EKINRGVAICHMDTSSWGPEHGAFV
Sbjct: 546 SVSCHQSLYPYLLYYCHSVPKVRIYEADILRLKTNEKINRGVAICHMDTSSWGPEHGAFV 605

Query: 601 ALGSGPGQIEVCHWIFENDMSWSVAD 621
           ALGSGPGQIEVCHWIFEND+SWSVAD
Sbjct: 606 ALGSGPGQIEVCHWIFENDVSWSVAD 626