Lsi05G018600 (gene) Bottle gourd (USVL1VR-Ls)

Overview
NameLsi05G018600
Typegene
OrganismLagenaria siceraria (Bottle gourd (USVL1VR-Ls))
DescriptionChaperone protein dnaJ 20
Locationchr05 : 25829776 .. 25831793 (+)
Genome Viewer
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
TTTTAATTCTAAACCGTACAAGTGACTCAGCCTTGGACGTCCCGTATAAATGTAGAAGATCCAGAGAACGGAAGATAGACGGAGAAAAACAGAGAAATGCAGTGCTCCGATTTTACCTTATCAGGGAGCGATTCACGCTTCTACATCCCTTCGAACCCTAGCACTACCGGCCGCCGACCAATTTCCGGCCACCGATCTCGGATATTCTTTCCCAACACCCTGCCGTGCAATTCGACGCGCTTGCCGTCTCTTTCAATCAGAGCCAAGGCCTCGTACAACGAAGGAATCCTCTCCTCCGAGGTCGCCGAAGGCTCCTTCTACGATTTGCTCGGTATATCTCAGTCCGGATCCTTGGCGGAAATCAAGCACGCGTACAAGCAGCTCGCTCGGAAGTACCATCCGGACGTGTCGCCGCCGGGCCGTGCGGAGGAGTATACGAAGACGTTCATTCGTGTTCAGGAGGCGTACGAGACATTGTCGGATCCGAGGAGGAGAGCGCTGTATGATAGAGATATGGTTGGAGGCCTTCAAGTCGCGTTCTCTGCTCGGAGACGATACGACGCCGATGAGGTATTAGTTCAGTCTACCAATTTTAACTTTGTGTTTTTTTCGTTATTTTAGGGGTAATAATTATGTTTGATTGTTTTTTCGTTTTTTTAGGGGTAATTATGTTTGATTGTTAACAGATTTTGAAATCTCTCGAGTTTCTTTTGCGTTTGCCCTTATTTTTGAAGTGATTTCGTGTTTAATTTCTCAGATGTTGTACAGAGAGATTAGAATGAGTGAAGGATTCGATCTTAATGTTTCTTTGATTTTGGATTTTTGGAGTGACGAAACGAAACGTCGTTTCATTCTGGGTCCTGATTCTCTTTTTCTTTCCTTTTTTGTTCGTCTGACTCTATCCTATACGGATGCAGTAGGATTGGTGGAGACGGATCCTGTTTCTGTTTAACAAGGACCTACAATTGGTTAATCAGTGATTTGTTCTGTAATTAAGAAACGGAATGGGATTGGTCAATCTGCTGTTTTCTGGATATCTTACCCCAAATATGGAATTCCGTTACCATAAACTTTAATTTTCATTACTCTTCTGTTTATCTATATGCAACTTTTTCACATAATATTTCTTTCTGTCTTCATTTAATTGTTGTATTCTAGCAAATCTGTATGAAATCCTTTTTCACCCACTGATTCAAACAAAACATTTGAGTTAATCCCACCTGGGAGTCCTGTTATCTCATTAGAACACATGCCAATTTTTTACTTCCCATTTGAATATCATACACTAAATCTCCAAATTTGATTTTGAATTCAGGAAGTTCCAGAGAGAAGTGGATGGAAAAATTGCTGGCAAGCTCAGATCTCAGAATTGAAGAGAAGAAGCATGGACAAGGATTCGAGACAAAATATGTCATGGGGAGCTCGAATGCGCCGGCAAATGAATGAACAGGTCTGATTGAATTATATAGATTAAGAAAACCATGTTTGACTTTCAGCATCAGTAAATATAATACGGCCTATTTAGTAACAATAATTTTGTTGTTAGTTTTCTCAATTTCCCCTTTGTAATCTGTGCTATACTATATATAAAAAGGGTTATGAGTGTAAAATCTTTGTTTCCCATCTTAGACCTCACAATTTTTCATAAATCCTACTTTGTCTTTCGTCACAACGTGTCGAGGTGATGGTTATTCTTTTATGTACCCGTGTAAGCTAGACCATATTTGAGTAGTACATTATGATCTAACAGCCCATCCAACTAGGATTGAACTCATGACCTCTTAGCCCTTTGTCAGGATCATTCCTCCTATCTAATAAGCCGACCAATGATAGTTTACAAAGAAAATCTAATAAGTTAAAAAATCAGAACCAAATTTGTAATTATATGGTCCCCAATTTTAGTTTTTTGAACTGAGACACAAAAAAATTGAAAGAGACCGTCAAGAGAGTGTTTAAGACGTCGAGTTGAGTTTTGAAATATGAAGTTAATATGATGGAGTTAGGTAGTTTGTGTTTGG

mRNA sequence

TTTTAATTCTAAACCGTACAAGTGACTCAGCCTTGGACGTCCCGTATAAATGTAGAAGATCCAGAGAACGGAAGATAGACGGAGAAAAACAGAGAAATGCAGTGCTCCGATTTTACCTTATCAGGGAGCGATTCACGCTTCTACATCCCTTCGAACCCTAGCACTACCGGCCGCCGACCAATTTCCGGCCACCGATCTCGGATATTCTTTCCCAACACCCTGCCGTGCAATTCGACGCGCTTGCCGTCTCTTTCAATCAGAGCCAAGGCCTCGTACAACGAAGGAATCCTCTCCTCCGAGGTCGCCGAAGGCTCCTTCTACGATTTGCTCGGTATATCTCAGTCCGGATCCTTGGCGGAAATCAAGCACGCGTACAAGCAGCTCGCTCGGAAGTACCATCCGGACGTGTCGCCGCCGGGCCGTGCGGAGGAGTATACGAAGACGTTCATTCGTGTTCAGGAGGCGTACGAGACATTGTCGGATCCGAGGAGGAGAGCGCTGTATGATAGAGATATGGTTGGAGGCCTTCAAGTCGCGTTCTCTGCTCGGAGACGATACGACGCCGATGAGGAAGTTCCAGAGAGAAGTGGATGGAAAAATTGCTGGCAAGCTCAGATCTCAGAATTGAAGAGAAGAAGCATGGACAAGGATTCGAGACAAAATATGTCATGGGGAGCTCGAATGCGCCGGCAAATGAATGAACAGGTCTGATTGAATTATATAGATTAAGAAAACCATGTTTGACTTTCAGCATCAGTAAATATAATACGGCCTATTTAGTAACAATAATTTTGTTGTTAGTTTTCTCAATTTCCCCTTTGTAATCTGTGCTATACTATATATAAAAAGGGTTATGAGTGTAAAATCTTTGTTTCCCATCTTAGACCTCACAATTTTTCATAAATCCTACTTTGTCTTTCGTCACAACGTGTCGAGGTGATGGTTATTCTTTTATGTACCCGTGTAAGCTAGACCATATTTGAGTAGTACATTATGATCTAACAGCCCATCCAACTAGGATTGAACTCATGACCTCTTAGCCCTTTGTCAGGATCATTCCTCCTATCTAATAAGCCGACCAATGATAGTTTACAAAGAAAATCTAATAAGTTAAAAAATCAGAACCAAATTTGTAATTATATGGTCCCCAATTTTAGTTTTTTGAACTGAGACACAAAAAAATTGAAAGAGACCGTCAAGAGAGTGTTTAAGACGTCGAGTTGAGTTTTGAAATATGAAGTTAATATGATGGAGTTAGGTAGTTTGTGTTTGG

Coding sequence (CDS)

ATGCAGTGCTCCGATTTTACCTTATCAGGGAGCGATTCACGCTTCTACATCCCTTCGAACCCTAGCACTACCGGCCGCCGACCAATTTCCGGCCACCGATCTCGGATATTCTTTCCCAACACCCTGCCGTGCAATTCGACGCGCTTGCCGTCTCTTTCAATCAGAGCCAAGGCCTCGTACAACGAAGGAATCCTCTCCTCCGAGGTCGCCGAAGGCTCCTTCTACGATTTGCTCGGTATATCTCAGTCCGGATCCTTGGCGGAAATCAAGCACGCGTACAAGCAGCTCGCTCGGAAGTACCATCCGGACGTGTCGCCGCCGGGCCGTGCGGAGGAGTATACGAAGACGTTCATTCGTGTTCAGGAGGCGTACGAGACATTGTCGGATCCGAGGAGGAGAGCGCTGTATGATAGAGATATGGTTGGAGGCCTTCAAGTCGCGTTCTCTGCTCGGAGACGATACGACGCCGATGAGGAAGTTCCAGAGAGAAGTGGATGGAAAAATTGCTGGCAAGCTCAGATCTCAGAATTGAAGAGAAGAAGCATGGACAAGGATTCGAGACAAAATATGTCATGGGGAGCTCGAATGCGCCGGCAAATGAATGAACAGGTCTGA

Protein sequence

MQCSDFTLSGSDSRFYIPSNPSTTGRRPISGHRSRIFFPNTLPCNSTRLPSLSIRAKASYNEGILSSEVAEGSFYDLLGISQSGSLAEIKHAYKQLARKYHPDVSPPGRAEEYTKTFIRVQEAYETLSDPRRRALYDRDMVGGLQVAFSARRRYDADEEVPERSGWKNCWQAQISELKRRSMDKDSRQNMSWGARMRRQMNEQV
Homology
[Close]
BLAST of Lsi05G018600 vs. Swiss-Prot
Match: DNJ20_ARATH (Chaperone protein dnaJ 20, chloroplastic OS=Arabidopsis thaliana GN=ATJ20 PE=1 SV=2)

HSP 1 Score: 171.8 bits (434), Expect = 7.8e-42
Identity = 95/158 (60.13%), Postives = 114/158 (72.15%), Query Frame = 1 

Query: 47  TRLPSLSIRAKASYNEGILSSEVAEGSFYDLLGISQSGSLAEIKHAYKQLARKYHPDVSP 106
           TR  S  I+++ ++++ +  SE  + SFYDLLG+++S +L EIK AYKQLARKYHPDVSP
Sbjct: 42  TRFSSTRIQSRLTHDDPVKQSE--DLSFYDLLGVTESVTLPEIKQAYKQLARKYHPDVSP 101

Query: 107 PGRAEEYTKTFIRVQEAYETLSDPRRRALYDRDMVGGLQVAFSARR--RYDADEEVPERS 166
           P R EEYT  FIRVQEAYETLSDPRRR LYDRD+  G   +FS RR  RYD  E V E+S
Sbjct: 102 PDRVEEYTDRFIRVQEAYETLSDPRRRVLYDRDLSMGFSFSFSGRRQNRYD-QEVVEEKS 161

Query: 167 GWKNCWQAQISELKRRSMDKDSRQNMSWGARMRRQMNE 203
            WK  WQ Q+S L+RRS  KD+   MSW ARMRRQ  E
Sbjct: 162 EWKAKWQTQLSGLRRRSNQKDN-NTMSWAARMRRQQQE 195

[Close]
BLAST of Lsi05G018600 vs. Swiss-Prot
Match: DNAJ_THEAB (Chaperone protein DnaJ OS=Thermosipho africanus (strain TCF52B) GN=dnaJ PE=3 SV=1)

HSP 1 Score: 73.6 bits (179), Expect = 2.9e-12
Identity = 35/70 (50.00%), Postives = 50/70 (71.43%), Query Frame = 1 

Query: 69  VAEGSFYDLLGISQSGSLAEIKHAYKQLARKYHPDVSPPGRAEEYTKTFIRVQEAYETLS 128
           +A+  +Y++LG+S++ S  EI+ AYKQL +K+HPD +   R E   K F  +QEAYE LS
Sbjct: 1   MAKKDYYEILGVSRNASQEEIRQAYKQLIKKWHPDRNHQNRKEAEEK-FKEIQEAYEVLS 60

Query: 129 DPRRRALYDR 139
           DP +RA+YDR
Sbjct: 61  DPEKRAMYDR 69

[Close]
BLAST of Lsi05G018600 vs. Swiss-Prot
Match: DNAJ_LACRD (Chaperone protein DnaJ OS=Lactobacillus reuteri (strain DSM 20016) GN=dnaJ PE=3 SV=1)

HSP 1 Score: 73.2 bits (178), Expect = 3.8e-12
Identity = 39/79 (49.37%), Postives = 53/79 (67.09%), Query Frame = 1 

Query: 69  VAEGSFYDLLGISQSGSLAEIKHAYKQLARKYHPDVSPPGRAEEYTKTFIRVQEAYETLS 128
           +AE  +YD+LG+S+  S  +IK AY++LA KYHPDV+    AEE    F ++ EAYETLS
Sbjct: 1   MAEQDYYDILGVSKDASEKDIKRAYRRLAAKYHPDVNHEPGAEE---KFKKINEAYETLS 60

Query: 129 DPRRRALYDRDMVGGLQVA 148
           D ++RA YD+    G Q A
Sbjct: 61  DSQKRAQYDQFGSAGPQGA 76

[Close]
BLAST of Lsi05G018600 vs. Swiss-Prot
Match: DNAJ_LACRJ (Chaperone protein DnaJ OS=Lactobacillus reuteri (strain JCM 1112) GN=dnaJ PE=3 SV=1)

HSP 1 Score: 73.2 bits (178), Expect = 3.8e-12
Identity = 39/79 (49.37%), Postives = 53/79 (67.09%), Query Frame = 1 

Query: 69  VAEGSFYDLLGISQSGSLAEIKHAYKQLARKYHPDVSPPGRAEEYTKTFIRVQEAYETLS 128
           +AE  +YD+LG+S+  S  +IK AY++LA KYHPDV+    AEE    F ++ EAYETLS
Sbjct: 1   MAEQDYYDILGVSKDASEKDIKRAYRRLAAKYHPDVNHEPGAEE---KFKKINEAYETLS 60

Query: 129 DPRRRALYDRDMVGGLQVA 148
           D ++RA YD+    G Q A
Sbjct: 61  DSQKRAQYDQFGSAGPQGA 76

[Close]
BLAST of Lsi05G018600 vs. Swiss-Prot
Match: DNAJ_LACF3 (Chaperone protein DnaJ OS=Lactobacillus fermentum (strain NBRC 3956 / LMG 18251) GN=dnaJ PE=3 SV=1)

HSP 1 Score: 73.2 bits (178), Expect = 3.8e-12
Identity = 39/77 (50.65%), Postives = 51/77 (66.23%), Query Frame = 1 

Query: 69  VAEGSFYDLLGISQSGSLAEIKHAYKQLARKYHPDVSPPGRAEEYTKTFIRVQEAYETLS 128
           +AE   YD+LG+ +  S AEIK AY++LA KYHPDV+    AE   K F ++ EAYETLS
Sbjct: 1   MAEEDLYDVLGVKKDASEAEIKRAYRKLAAKYHPDVNHEPGAE---KKFKKINEAYETLS 60

Query: 129 DPRRRALYDRDMVGGLQ 146
           D ++RA YD+    G Q
Sbjct: 61  DDQKRAQYDQFGTTGSQ 74

[Close]
BLAST of Lsi05G018600 vs. TrEMBL
Match: A0A0A0L4L9_CUCSA (Chaperone protein dnaJ 20, chloroplast OS=Cucumis sativus GN=Csa_3G129700 PE=4 SV=1)

HSP 1 Score: 346.3 bits (887), Expect = 2.6e-92
Identity = 172/203 (84.73%), Postives = 186/203 (91.63%), Query Frame = 1 

Query: 1   MQCSDFTLSGSDSRFYIPSNPSTTGRRPISGHRSRIFFPNTLPCNSTRLPSLSIRAKASY 60
           MQCSD T SGSDSRFYIPSNP+TT RRPISG+RSR+ FP+T P NSTRLPSLSIRAKAS+
Sbjct: 14  MQCSDLTFSGSDSRFYIPSNPTTTARRPISGYRSRVCFPHTPPFNSTRLPSLSIRAKASF 73

Query: 61  NEGILSSEVAEGSFYDLLGISQSGSLAEIKHAYKQLARKYHPDVSPPGRAEEYTKTFIRV 120
           NEG +SSEVAEGSFYDLLGIS+SGSL EIK AYKQLARKYHPDVSPPG  EE TK FIRV
Sbjct: 74  NEGFVSSEVAEGSFYDLLGISKSGSLEEIKRAYKQLARKYHPDVSPPGCVEENTKRFIRV 133

Query: 121 QEAYETLSDPRRRALYDRDMVGGLQVAFSARRRYDADEEVPERSGWKNCWQAQISELKRR 180
           QEAYETL+DPRRRALYDRDM+GGLQVAFSARRRYDADEEV ++SGW+N W+AQISELKRR
Sbjct: 134 QEAYETLADPRRRALYDRDMIGGLQVAFSARRRYDADEEVAQKSGWRNSWEAQISELKRR 193

Query: 181 SMDKDSRQNMSWGARMRRQMNEQ 204
           SM+KD R NMSWGARMRRQMNEQ
Sbjct: 194 SMEKDLRPNMSWGARMRRQMNEQ 216

[Close]
BLAST of Lsi05G018600 vs. TrEMBL
Match: W9S291_9ROSA (Chaperone protein dnaJ 20 OS=Morus notabilis GN=L484_011075 PE=4 SV=1)

HSP 1 Score: 236.1 bits (601), Expect = 3.7e-59
Identity = 125/203 (61.58%), Postives = 151/203 (74.38%), Query Frame = 1 

Query: 1   MQCSDFTLS-GSDSRFYIPSNPSTTGRRPISGHRSRIFFPNTLPCNSTRLPSLSIRAKAS 60
           M+C+   +S GSDSRF+ P+N ST          S + FP   P  STR+ +     +AS
Sbjct: 1   MRCNGLAISGGSDSRFFFPTNSSTIYTASARNPSSTVLFPGR-PVFSTRIRA---TPRAS 60

Query: 61  YNEGILSSEVAEGSFYDLLGISQSGSLAEIKHAYKQLARKYHPDVSPPGRAEEYTKTFIR 120
               ++   VAE SFY+LLGI +SGSL EIK AYKQLARKYHPDVSPPGR EEYT+TFIR
Sbjct: 61  AAAAVVGDRVAELSFYELLGIPESGSLGEIKQAYKQLARKYHPDVSPPGRVEEYTRTFIR 120

Query: 121 VQEAYETLSDPRRRALYDRDMVGGLQVAFSARRRYDADEEVPERSGWKNCWQAQISELKR 180
           VQEAYETLSDPR RA+YDRDM  GL +AFSARRRY +DE + E+S WK+ WQ+Q+SELKR
Sbjct: 121 VQEAYETLSDPRLRAIYDRDMAKGLHLAFSARRRYQSDEGMEEKSEWKSHWQSQLSELKR 180

Query: 181 RSMDKDSRQNMSWGARMRRQMNE 203
           RS++KDSR NMSWGARMRR+M +
Sbjct: 181 RSVNKDSRGNMSWGARMRRRMED 199

[Close]
BLAST of Lsi05G018600 vs. TrEMBL
Match: A0A061EJK6_THECC (Chaperone protein dnaJ 20 OS=Theobroma cacao GN=TCM_020242 PE=4 SV=1)

HSP 1 Score: 215.3 bits (547), Expect = 6.8e-53
Identity = 115/200 (57.50%), Postives = 140/200 (70.00%), Query Frame = 1 

Query: 3   CSDFTLSGSDSRFYIPSNPSTTGRRPISGHRSRIFFPNTLPCNSTRLPSLSIRAKASYNE 62
           C    + G+D+RF++P       RRP+               + TR+   S++ KA  NE
Sbjct: 5   CGLAAIPGTDARFFVPDTSFIRLRRPV---------------DPTRVQFGSLKIKARINE 64

Query: 63  GILSSEVAEGSFYDLLGISQSGSLAEIKHAYKQLARKYHPDVSPPGRAEEYTKTFIRVQE 122
                   E SFY+LLGI ++G+L EIK AYKQLARKYHPDVSPP R EEYT+ FIRVQE
Sbjct: 65  AR-----KEVSFYELLGIPETGTLPEIKQAYKQLARKYHPDVSPPDRVEEYTQRFIRVQE 124

Query: 123 AYETLSDPRRRALYDRDMVGGLQVAFSARRRYDADEEVPERSGWKNCWQAQISELKRRSM 182
           AYETLSDPRRRA YDRD+  G+ +AFSARRRY  DEE+ ERS WKN WQ+Q+SELKRRSM
Sbjct: 125 AYETLSDPRRRASYDRDLALGIHLAFSARRRYQYDEELEERSEWKNRWQSQLSELKRRSM 184

Query: 183 DKDSRQNMSWGARMRRQMNE 203
           ++D+R NMSWGARMRRQ +E
Sbjct: 185 NRDARGNMSWGARMRRQRDE 184

[Close]
BLAST of Lsi05G018600 vs. TrEMBL
Match: B9RWC0_RICCO (Chaperone protein dnaJ 20, chloroplast, putative OS=Ricinus communis GN=RCOM_1017840 PE=4 SV=1)

HSP 1 Score: 211.8 bits (538), Expect = 7.6e-52
Identity = 114/199 (57.29%), Postives = 138/199 (69.35%), Query Frame = 1 

Query: 1   MQCSDFTLSGSDSRFYIPSNPSTTGRRPISGHRSRIFFPNTLPCNSTRLPSLSIRAKASY 60
           M C++  L G     Y     S++   PI+  +   F     P   TR P  S + KA+ 
Sbjct: 1   MHCTNLMLPGGSDTVYFFHPSSSSSTTPITKSKPGPFL-RLKPV--TRFPMSSFKTKAAV 60

Query: 61  NEGILSSEVAEGSFYDLLGISQSGSLAEIKHAYKQLARKYHPDVSPPGRAEEYTKTFIRV 120
           ++ I + ++   SFY+LLGI +SGSL +IK AYKQLARKYHPDVSPP R  EYT+ FI+V
Sbjct: 61  SDRIATKDL---SFYELLGIPESGSLIDIKQAYKQLARKYHPDVSPPDRVHEYTQRFIQV 120

Query: 121 QEAYETLSDPRRRALYDRDMVGGLQVAFSARRRYDADEEVPERSGWKNCWQAQISELKRR 180
           QEAYETLSDPRRRA YDRDM  GL +AFSARRRY +DEEV   S WKN W+AQ+SELKRR
Sbjct: 121 QEAYETLSDPRRRATYDRDMARGLHLAFSARRRYQSDEEVEVSSDWKNRWEAQLSELKRR 180

Query: 181 SMDKDSRQNMSWGARMRRQ 200
           SM+KDS +NMSW ARMRRQ
Sbjct: 181 SMNKDSEENMSWAARMRRQ 193

[Close]
BLAST of Lsi05G018600 vs. TrEMBL
Match: A0A059B1U7_EUCGR (Uncharacterized protein OS=Eucalyptus grandis GN=EUGRSUZ_H02689 PE=4 SV=1)

HSP 1 Score: 209.5 bits (532), Expect = 3.8e-51
Identity = 116/203 (57.14%), Postives = 138/203 (67.98%), Query Frame = 1 

Query: 1   MQCSDFTLSGSD-SRFYIPSNPSTTGRRPISGHRSRIFFPNTLPCNSTRLPSLSIRAKAS 60
           M+C    L G D SR  +P  P         GHR+  F P  +   + R  +  I   A 
Sbjct: 1   MRCYGVALPGGDPSRLCLPPPPPPFPSARTHGHRA--FLPTRVRFGAVRCAAGRIDDGAV 60

Query: 61  YNEGILSSEVAEGSFYDLLGISQSGSLAEIKHAYKQLARKYHPDVSPPGRAEEYTKTFIR 120
            +    S+  AE SFYDLLGI +SG+L EIK AYKQLARKYHPDVSPPGR EEYT+ FIR
Sbjct: 61  ADAARCSA--AEASFYDLLGIPESGTLPEIKQAYKQLARKYHPDVSPPGRVEEYTQRFIR 120

Query: 121 VQEAYETLSDPRRRALYDRDMVGGLQVAFSARRRYDADEEVPERSGWKNCWQAQISELKR 180
           VQEAYETLSDPRRRA+YDRD+  G+ +AFSARR Y  DE++ ++S WK+ WQ Q+S LKR
Sbjct: 121 VQEAYETLSDPRRRAVYDRDLARGIHLAFSARRPYRGDEDLEDKSEWKSRWQVQLSGLKR 180

Query: 181 RSMDKDSRQNMSWGARMRRQMNE 203
           RS  KDSR NMSWGARMR+QM E
Sbjct: 181 RSAHKDSRGNMSWGARMRKQMEE 199

[Close]
BLAST of Lsi05G018600 vs. TAIR10
Match: AT4G13830.2 (AT4G13830.2 DNAJ-like 20)

HSP 1 Score: 171.8 bits (434), Expect = 4.4e-43
Identity = 95/158 (60.13%), Postives = 114/158 (72.15%), Query Frame = 1 

Query: 47  TRLPSLSIRAKASYNEGILSSEVAEGSFYDLLGISQSGSLAEIKHAYKQLARKYHPDVSP 106
           TR  S  I+++ ++++ +  SE  + SFYDLLG+++S +L EIK AYKQLARKYHPDVSP
Sbjct: 42  TRFSSTRIQSRLTHDDPVKQSE--DLSFYDLLGVTESVTLPEIKQAYKQLARKYHPDVSP 101

Query: 107 PGRAEEYTKTFIRVQEAYETLSDPRRRALYDRDMVGGLQVAFSARR--RYDADEEVPERS 166
           P R EEYT  FIRVQEAYETLSDPRRR LYDRD+  G   +FS RR  RYD  E V E+S
Sbjct: 102 PDRVEEYTDRFIRVQEAYETLSDPRRRVLYDRDLSMGFSFSFSGRRQNRYD-QEVVEEKS 161

Query: 167 GWKNCWQAQISELKRRSMDKDSRQNMSWGARMRRQMNE 203
            WK  WQ Q+S L+RRS  KD+   MSW ARMRRQ  E
Sbjct: 162 EWKAKWQTQLSGLRRRSNQKDN-NTMSWAARMRRQQQE 195

[Close]
BLAST of Lsi05G018600 vs. TAIR10
Match: AT4G39960.1 (AT4G39960.1 Molecular chaperone Hsp40/DnaJ family protein)

HSP 1 Score: 71.6 bits (174), Expect = 6.2e-13
Identity = 43/119 (36.13%), Postives = 63/119 (52.94%), Query Frame = 1 

Query: 29  ISGHRSRIFFPNTLPCNSTRLPSLSIRAKASYNEGILSSEVAEGSFYDLLGISQSGSLAE 88
           +   RS +F   +LP     L    +        G   +  A+  FY +LG+S++ + AE
Sbjct: 45  LGASRSSMFAQGSLPF----LSLTGVSPNTHSRRGARFTVRADTDFYSVLGVSKNATKAE 104

Query: 89  IKHAYKQLARKYHPDVSPPGRAEEYTKTFIRVQEAYETLSDPRRRALYDRDMVGGLQVA 148
           IK AY++LAR YHPDV+    AE+    F  +  AYE LSD  +R+LYDR    G++ A
Sbjct: 105 IKSAYRKLARSYHPDVNKDAGAED---KFKEISNAYEILSDDEKRSLYDRYGEAGVKGA 156

[Close]
BLAST of Lsi05G018600 vs. TAIR10
Match: AT2G22360.1 (AT2G22360.1 DNAJ heat shock family protein)

HSP 1 Score: 70.5 bits (171), Expect = 1.4e-12
Identity = 36/78 (46.15%), Postives = 51/78 (65.38%), Query Frame = 1 

Query: 70  AEGSFYDLLGISQSGSLAEIKHAYKQLARKYHPDVSPPGRAEEYTKTFIRVQEAYETLSD 129
           A+  +Y +LG+S++ + AEIK AY++LAR YHPDV+    AEE    F  +  AYE LSD
Sbjct: 83  ADADYYSVLGVSKNATKAEIKSAYRKLARNYHPDVNKDPGAEE---KFKEISNAYEVLSD 142

Query: 130 PRRRALYDRDMVGGLQVA 148
             +++LYDR    GL+ A
Sbjct: 143 DEKKSLYDRYGEAGLKGA 157

[Close]
BLAST of Lsi05G018600 vs. TAIR10
Match: AT3G17830.1 (AT3G17830.1 Molecular chaperone Hsp40/DnaJ family protein)

HSP 1 Score: 67.0 bits (162), Expect = 1.5e-11
Identity = 46/134 (34.33%), Postives = 71/134 (52.99%), Query Frame = 1 

Query: 17  IPS-NPSTTGRRPISGHRSRIFFPNTLPCNSTRLPSLSIRAKASYNEGILSSEVAEGSFY 76
           IPS NP+ + R       +  + P TLP  S R+    I     Y   +++   A    Y
Sbjct: 10  IPSINPAHSFRHQFPQPNASFYLPPTLPIFS-RVRRFGI--SGGYRRRVITM-AAGTDHY 69

Query: 77  DLLGISQSGSLAEIKHAYKQLARKYHPDVSPPGRAEEYTKTFIRVQEAYETLSDPRRRAL 136
             L ++++ +L EIK +Y++LARKYHPD++    AE+    F ++  AYE LSD  +R+ 
Sbjct: 70  STLNVNRNATLQEIKSSYRKLARKYHPDMNKNPGAED---KFKQISAAYEVLSDEEKRSA 129

Query: 137 YDRDMVGGLQVAFS 150
           YDR    GL+  F+
Sbjct: 130 YDRFGEAGLEGDFN 136

[Close]
BLAST of Lsi05G018600 vs. TAIR10
Match: AT1G80030.1 (AT1G80030.1 Molecular chaperone Hsp40/DnaJ family protein)

HSP 1 Score: 65.5 bits (158), Expect = 4.4e-11
Identity = 34/68 (50.00%), Postives = 47/68 (69.12%), Query Frame = 1 

Query: 72  GSFYDLLGISQSGSLAEIKHAYKQLARKYHPDVS-PPGRAEEYTKTFIRVQEAYETLSDP 131
           G +Y  LG+S+S +  EIK AY++LAR+YHPDV+  PG  E+    F  +  AYE LSD 
Sbjct: 74  GDYYATLGVSKSANNKEIKAAYRRLARQYHPDVNKEPGATEK----FKEISAAYEVLSDE 133

Query: 132 RRRALYDR 139
           ++RALYD+
Sbjct: 134 QKRALYDQ 137

[Close]
BLAST of Lsi05G018600 vs. NCBI nr
Match: gi|659075782|ref|XP_008438327.1| (PREDICTED: chaperone protein dnaJ 20, chloroplastic-like [Cucumis melo])

HSP 1 Score: 359.8 bits (922), Expect = 3.2e-96
Identity = 178/204 (87.25%), Postives = 191/204 (93.63%), Query Frame = 1 

Query: 1   MQCSDFTLSGSDSRFYIPSNPSTTGRRPISGHRSRIFFPNTLPCNSTRLPSLSIRAKASY 60
           MQCSDFT SGSDSRFYIPSNP+ + RRPISG+RSR+FFP+T P NSTRLPSLSIRAKAS+
Sbjct: 27  MQCSDFTFSGSDSRFYIPSNPTISARRPISGYRSRVFFPHTPPFNSTRLPSLSIRAKASF 86

Query: 61  NEGILSSEVAEGSFYDLLGISQSGSLAEIKHAYKQLARKYHPDVSPPGRAEEYTKTFIRV 120
           NEG +SSEVAEGSFYDLLGISQSGSLAEIK AYKQLARKYHPDVSPPG AEEYTK FIRV
Sbjct: 87  NEGFVSSEVAEGSFYDLLGISQSGSLAEIKRAYKQLARKYHPDVSPPGGAEEYTKMFIRV 146

Query: 121 QEAYETLSDPRRRALYDRDMVGGLQVAFSARRRYDADEEVPERSGWKNCWQAQISELKRR 180
           QEAYETLSDPRRRALYDRDM+GGLQVAFSARRRYDA EEV E+SGW+N W+AQISELKRR
Sbjct: 147 QEAYETLSDPRRRALYDRDMIGGLQVAFSARRRYDAAEEVAEKSGWRNSWEAQISELKRR 206

Query: 181 SMDKDSRQNMSWGARMRRQMNEQV 205
           SM+KD RQNMSWGARMRRQMNEQ+
Sbjct: 207 SMEKDLRQNMSWGARMRRQMNEQI 230

[Close]
BLAST of Lsi05G018600 vs. NCBI nr
Match: gi|449432390|ref|XP_004133982.1| (PREDICTED: chaperone protein dnaJ 20, chloroplastic-like [Cucumis sativus])

HSP 1 Score: 346.3 bits (887), Expect = 3.7e-92
Identity = 172/203 (84.73%), Postives = 186/203 (91.63%), Query Frame = 1 

Query: 1   MQCSDFTLSGSDSRFYIPSNPSTTGRRPISGHRSRIFFPNTLPCNSTRLPSLSIRAKASY 60
           MQCSD T SGSDSRFYIPSNP+TT RRPISG+RSR+ FP+T P NSTRLPSLSIRAKAS+
Sbjct: 14  MQCSDLTFSGSDSRFYIPSNPTTTARRPISGYRSRVCFPHTPPFNSTRLPSLSIRAKASF 73

Query: 61  NEGILSSEVAEGSFYDLLGISQSGSLAEIKHAYKQLARKYHPDVSPPGRAEEYTKTFIRV 120
           NEG +SSEVAEGSFYDLLGIS+SGSL EIK AYKQLARKYHPDVSPPG  EE TK FIRV
Sbjct: 74  NEGFVSSEVAEGSFYDLLGISKSGSLEEIKRAYKQLARKYHPDVSPPGCVEENTKRFIRV 133

Query: 121 QEAYETLSDPRRRALYDRDMVGGLQVAFSARRRYDADEEVPERSGWKNCWQAQISELKRR 180
           QEAYETL+DPRRRALYDRDM+GGLQVAFSARRRYDADEEV ++SGW+N W+AQISELKRR
Sbjct: 134 QEAYETLADPRRRALYDRDMIGGLQVAFSARRRYDADEEVAQKSGWRNSWEAQISELKRR 193

Query: 181 SMDKDSRQNMSWGARMRRQMNEQ 204
           SM+KD R NMSWGARMRRQMNEQ
Sbjct: 194 SMEKDLRPNMSWGARMRRQMNEQ 216

[Close]
BLAST of Lsi05G018600 vs. NCBI nr
Match: gi|703148387|ref|XP_010109333.1| (Chaperone protein dnaJ 20 [Morus notabilis])

HSP 1 Score: 236.1 bits (601), Expect = 5.4e-59
Identity = 125/203 (61.58%), Postives = 151/203 (74.38%), Query Frame = 1 

Query: 1   MQCSDFTLS-GSDSRFYIPSNPSTTGRRPISGHRSRIFFPNTLPCNSTRLPSLSIRAKAS 60
           M+C+   +S GSDSRF+ P+N ST          S + FP   P  STR+ +     +AS
Sbjct: 1   MRCNGLAISGGSDSRFFFPTNSSTIYTASARNPSSTVLFPGR-PVFSTRIRA---TPRAS 60

Query: 61  YNEGILSSEVAEGSFYDLLGISQSGSLAEIKHAYKQLARKYHPDVSPPGRAEEYTKTFIR 120
               ++   VAE SFY+LLGI +SGSL EIK AYKQLARKYHPDVSPPGR EEYT+TFIR
Sbjct: 61  AAAAVVGDRVAELSFYELLGIPESGSLGEIKQAYKQLARKYHPDVSPPGRVEEYTRTFIR 120

Query: 121 VQEAYETLSDPRRRALYDRDMVGGLQVAFSARRRYDADEEVPERSGWKNCWQAQISELKR 180
           VQEAYETLSDPR RA+YDRDM  GL +AFSARRRY +DE + E+S WK+ WQ+Q+SELKR
Sbjct: 121 VQEAYETLSDPRLRAIYDRDMAKGLHLAFSARRRYQSDEGMEEKSEWKSHWQSQLSELKR 180

Query: 181 RSMDKDSRQNMSWGARMRRQMNE 203
           RS++KDSR NMSWGARMRR+M +
Sbjct: 181 RSVNKDSRGNMSWGARMRRRMED 199

[Close]
BLAST of Lsi05G018600 vs. NCBI nr
Match: gi|1009139653|ref|XP_015887231.1| (PREDICTED: chaperone protein dnaJ 20, chloroplastic [Ziziphus jujuba])

HSP 1 Score: 218.8 bits (556), Expect = 8.9e-54
Identity = 122/212 (57.55%), Postives = 144/212 (67.92%), Query Frame = 1 

Query: 1   MQCSDFTLSGSDSRFYI----PSNPSTTGRR------PISGHRSRIFFPNTLPCNSTRLP 60
           M+    T+SGSDSRF+     P N S           P   HR  IF   T     TR  
Sbjct: 1   MRSHGLTISGSDSRFFSFPVKPVNKSIPDPNHNVLFFPAHHHRPIIFTTTT-----TRDS 60

Query: 61  SLSIRAKASYNEGILSSEVAEGSFYDLLGISQSGSLAEIKHAYKQLARKYHPDVSPPGRA 120
             ++R KA+ N+G + SE AE SFY+LLGI + GSL +IK AYKQLARKYHPDVSPPGR 
Sbjct: 61  IGTVRVKATVNDGFVFSEAAELSFYELLGIPEWGSLVDIKQAYKQLARKYHPDVSPPGRV 120

Query: 121 EEYTKTFIRVQEAYETLSDPRRRALYDRDMVGGLQVAFSARRRYDADEEVPERSGWKNCW 180
           EEYT+ FIRVQEAYETLSDPRRRALYDRDM  G+ +AFSAR+RY  DE + ++  WK  W
Sbjct: 121 EEYTQRFIRVQEAYETLSDPRRRALYDRDMAKGIHLAFSARKRYQKDEGMEDKGEWKYRW 180

Query: 181 QAQISELKRRSMDKDSRQNMSWGARMRRQMNE 203
           Q+Q+SELKRRS +KD   NMSWGARMRR+  E
Sbjct: 181 QSQLSELKRRSTNKDMTGNMSWGARMRRRREE 207

[Close]
BLAST of Lsi05G018600 vs. NCBI nr
Match: gi|470121999|ref|XP_004297043.1| (PREDICTED: chaperone protein dnaJ 20, chloroplastic-like [Fragaria vesca subsp. vesca])

HSP 1 Score: 216.1 bits (549), Expect = 5.8e-53
Identity = 120/199 (60.30%), Postives = 142/199 (71.36%), Query Frame = 1 

Query: 1   MQCSDFTLSGSDSRFYIPSNPSTTGRRPISGHRSRIFFPNTLPCNSTRLPSLSIRAKASY 60
           MQ    T+ G+DSR YI      +  RP    +     P++LP  S+R    SI+AKA+ 
Sbjct: 1   MQTYGLTIPGADSRLYITP---ISAARPAPDPK-----PSSLPVFSSRTRFGSIKAKAAT 60

Query: 61  NEGILSSEVAEGSFYDLLGISQSGSLAEIKHAYKQLARKYHPDVSPPGRAEEYTKTFIRV 120
             G +++E  E SFY+LLGI +SGS+ EIK AYKQLARKYHPDVSPPGR EEYT+ FIRV
Sbjct: 61  MNGAVTAEEKELSFYELLGIPESGSIKEIKQAYKQLARKYHPDVSPPGRVEEYTEKFIRV 120

Query: 121 QEAYETLSDPRRRALYDRDMVGGLQVAFSARRRYDADEEVPERSGWKNCWQAQISELKRR 180
           QEAYETLSD RRRALYD+DM  GL +AFSA RRY  DE +  +  WKN WQ+Q+SELKRR
Sbjct: 121 QEAYETLSDSRRRALYDQDMARGLHLAFSA-RRYQYDEGMEVKGDWKNRWQSQLSELKRR 180

Query: 181 SMDKDSRQNMSWGARMRRQ 200
           S  KD RQNMSWGARMRRQ
Sbjct: 181 SSYKDDRQNMSWGARMRRQ 190

The following BLAST results are available for this feature:
BLAST of Lsi05G018600 vs. Swiss-Prot
Match NameE-valueIdentityDescription
DNJ20_ARATH7.8e-4260.13Chaperone protein dnaJ 20, chloroplastic OS=Arabidopsis thaliana GN=ATJ20 PE=1 S... [more]
DNAJ_THEAB2.9e-1250.00Chaperone protein DnaJ OS=Thermosipho africanus (strain TCF52B) GN=dnaJ PE=3 SV=... [more]
DNAJ_LACRD3.8e-1249.37Chaperone protein DnaJ OS=Lactobacillus reuteri (strain DSM 20016) GN=dnaJ PE=3 ... [more]
DNAJ_LACRJ3.8e-1249.37Chaperone protein DnaJ OS=Lactobacillus reuteri (strain JCM 1112) GN=dnaJ PE=3 S... [more]
DNAJ_LACF33.8e-1250.65Chaperone protein DnaJ OS=Lactobacillus fermentum (strain NBRC 3956 / LMG 18251)... [more]
BLAST of Lsi05G018600 vs. TrEMBL
Match NameE-valueIdentityDescription
A0A0A0L4L9_CUCSA2.6e-9284.73Chaperone protein dnaJ 20, chloroplast OS=Cucumis sativus GN=Csa_3G129700 PE=4 S... [more]
W9S291_9ROSA3.7e-5961.58Chaperone protein dnaJ 20 OS=Morus notabilis GN=L484_011075 PE=4 SV=1[more]
A0A061EJK6_THECC6.8e-5357.50Chaperone protein dnaJ 20 OS=Theobroma cacao GN=TCM_020242 PE=4 SV=1[more]
B9RWC0_RICCO7.6e-5257.29Chaperone protein dnaJ 20, chloroplast, putative OS=Ricinus communis GN=RCOM_101... [more]
A0A059B1U7_EUCGR3.8e-5157.14Uncharacterized protein OS=Eucalyptus grandis GN=EUGRSUZ_H02689 PE=4 SV=1[more]
BLAST of Lsi05G018600 vs. TAIR10
Match NameE-valueIdentityDescription
AT4G13830.24.4e-4360.13 DNAJ-like 20[more]
AT4G39960.16.2e-1336.13 Molecular chaperone Hsp40/DnaJ family protein[more]
AT2G22360.11.4e-1246.15 DNAJ heat shock family protein[more]
AT3G17830.11.5e-1134.33 Molecular chaperone Hsp40/DnaJ family protein[more]
AT1G80030.14.4e-1150.00 Molecular chaperone Hsp40/DnaJ family protein[more]
BLAST of Lsi05G018600 vs. NCBI nr
Match NameE-valueIdentityDescription
gi|659075782|ref|XP_008438327.1|3.2e-9687.25PREDICTED: chaperone protein dnaJ 20, chloroplastic-like [Cucumis melo][more]
gi|449432390|ref|XP_004133982.1|3.7e-9284.73PREDICTED: chaperone protein dnaJ 20, chloroplastic-like [Cucumis sativus][more]
gi|703148387|ref|XP_010109333.1|5.4e-5961.58Chaperone protein dnaJ 20 [Morus notabilis][more]
gi|1009139653|ref|XP_015887231.1|8.9e-5457.55PREDICTED: chaperone protein dnaJ 20, chloroplastic [Ziziphus jujuba][more]
gi|470121999|ref|XP_004297043.1|5.8e-5360.30PREDICTED: chaperone protein dnaJ 20, chloroplastic-like [Fragaria vesca subsp. ... [more]
Annotated Terms
The following terms have been associated with this gene:
Vocabulary: INTERPRO
TermDefinition
IPR018253DnaJ_domain_CS
IPR001623DnaJ_domain
GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0008150 biological_process
biological_process GO:0008152 metabolic process
biological_process GO:0044237 cellular metabolic process
biological_process GO:0071704 organic substance metabolic process
biological_process GO:0044238 primary metabolic process
biological_process GO:0019222 regulation of metabolic process
cellular_component GO:0005575 cellular_component
cellular_component GO:0043231 intracellular membrane-bounded organelle
molecular_function GO:0003674 molecular_function
Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lsi05G018600.1Lsi05G018600.1mRNA


InterPro
Analysis Name: InterPro Annotations of Lagenaria siceraria
Date Performed: 2017-09-18
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001623DnaJ domainPRINTSPR00625JDOMAINcoord: 112..132
score: 7.7E-16coord: 132..151
score: 7.7E-16coord: 75..93
score: 7.7E-16coord: 93..108
score: 7.7
IPR001623DnaJ domainGENE3DG3DSA:1.10.287.110coord: 67..144
score: 2.1
IPR001623DnaJ domainPFAMPF00226DnaJcoord: 74..137
score: 2.2
IPR001623DnaJ domainSMARTSM00271dnaj_3coord: 72..132
score: 4.2
IPR001623DnaJ domainPROFILEPS50076DNAJ_2coord: 73..140
score: 19
IPR001623DnaJ domainunknownSSF46565Chaperone J-domaincoord: 73..146
score: 1.96
IPR018253DnaJ domain, conserved sitePROSITEPS00636DNAJ_1coord: 117..136
scor
NoneNo IPR availablePANTHERPTHR24078DNAJ HOMOLOG SUBFAMILY C MEMBERcoord: 74..138
score: 1.0
NoneNo IPR availablePANTHERPTHR24078:SF222SUBFAMILY NOT NAMEDcoord: 74..138
score: 1.0

Synteny
The following gene(s) are orthologous to this gene:
GeneOrthologueOrganismBlock
Lsi05G018600Csa3G129700Cucumber (Chinese Long) v2culsiB209
Lsi05G018600CSPI03G10650Wild cucumber (PI 183967)cpilsiB207
Lsi05G018600Cucsa.256640Cucumber (Gy14) v1cgylsiB415
Lsi05G018600CmaCh14G019070Cucurbita maxima (Rimu)cmalsiB245
Lsi05G018600CmaCh06G012000Cucurbita maxima (Rimu)cmalsiB764
Lsi05G018600CmoCh14G019620Cucurbita moschata (Rifu)cmolsiB233
Lsi05G018600CmoCh06G012370Cucurbita moschata (Rifu)cmolsiB757
Lsi05G018600CmoCh08G009710Cucurbita moschata (Rifu)cmolsiB823
Lsi05G018600Cla021401Watermelon (97103) v1lsiwmB365
Lsi05G018600MELO3C006295Melon (DHL92) v3.5.1lsimeB378
Lsi05G018600ClCG05G003140Watermelon (Charleston Gray)lsiwcgB335
Lsi05G018600MELO3C006295.2Melon (DHL92) v3.6.1lsimedB396
Lsi05G018600Cla97C05G083770Watermelon (97103) v2lsiwmbB333
Lsi05G018600Bhi01G000145Wax gourdlsiwgoB445
Lsi05G018600Cp4.1LG03g18080Cucurbita pepo (Zucchini)cpelsiB506
Lsi05G018600Cp4.1LG08g02660Cucurbita pepo (Zucchini)cpelsiB714
Lsi05G018600CsGy3G010700Cucumber (Gy14) v2cgyblsiB196
Lsi05G018600Carg06307Silver-seed gourdcarlsiB878
Lsi05G018600Carg11046Silver-seed gourdcarlsiB983
Lsi05G018600Carg10152Silver-seed gourdcarlsiB206
Lsi05G018600Carg24775Silver-seed gourdcarlsiB156
Lsi05G018600CsaV3_3G010760Cucumber (Chinese Long) v3cuclsiB228
The following gene(s) are paralogous to this gene:

None

The following block(s) are covering this gene:
GeneOrganismBlock
Lsi05G018600Bottle gourd (USVL1VR-Ls)lsilsiB011
Lsi05G018600Cucurbita maxima (Rimu)cmalsiB356
Lsi05G018600Cucurbita moschata (Rifu)cmolsiB345
Lsi05G018600Wild cucumber (PI 183967)cpilsiB124
Lsi05G018600Wild cucumber (PI 183967)cpilsiB128
Lsi05G018600Melon (DHL92) v3.5.1lsimeB360
Lsi05G018600Watermelon (Charleston Gray)lsiwcgB343
Lsi05G018600Cucumber (Gy14) v2cgyblsiB114
Lsi05G018600Watermelon (97103) v2lsiwmbB341
Lsi05G018600Wax gourdlsiwgoB421