Bhi01G000145 (gene) Wax gourd

Overview
NameBhi01G000145
Typegene
OrganismBenincasa hispida (Wax gourd)
DescriptionChaperone protein DnaJ
Locationchr1 : 3819471 .. 3821217 (-)
Genome Viewer
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
GTACAGATGACTCAGCCTTGGACGTCCCGTATAAATAGCAGAAGATGCAGAGAACGGAATATAGACGGAGAAAAACAGAGAAATGCAGTGCTCTGATTTTACCTTTTCAGGGAGCGATTCACGCTTCTACATCCCTTCGAACCCTAACATTACCGGTCGCCGACCAATTCCCTGCCACCGATCTCGGGTTTTCTTTCCCAGCACTCCGCCGTGCAATTCGACGCGCTCGCCGCCTCTTTCAATCAGAGCCAAGGCCTCGTTCAACGAAGGAACCGTCTCCTCCGAGGTCGCCGAAGGCTCCTTCTACGATTTGCTCGGTATATCTCAGTCCGGATCCTTGGAGGAAATCAAGCACGCGTACAAGCAACTCGCTCGGAAGTACCATCCGGACGTGTCGCCGCCGGGGCGTGCGGAGGAGTATACGAAGAGGTTCATTCGTGTTCAGGAGGCGTACGAGACATTGTCGGATCCGAGGAGGAGAGCGCTGTATGATAGAGATATGGTTGGAGGTCTTCAAGTCGCATTCTCTGGTCGGAGACGATACGACGCCCATGAGGTATTAGTTCAGTCTACCGATTTTAACTTTGTGTTTTCTCGGTATTTTAGGCGTAATTATGTTTGATTGTTGGCGGATTTTGAAATCTCCTGTGTTTCTTTTGTGTTTACCTTGTTTTTTTGAAGTGATTTCGTGTTTAATTTCTCAGATCTTGTCCAGAGAGATTAGAATGAGTGAAGGATTTGATCTCAATGTTCTTTGATTTTGGATTTTTAGAGCGAGGAAAGGAAACGTCGTTATTCTGGGTCCTGATTCTTTTTATCTTTCCTTATTTGTTCTTCTGACTCTATCTTATACGGATTCAATAGGATTGGTGGAGAGCGATTCTGTTTCTGTTTAAACAAGGACCTACCATTCGTTATTCAGTGATTTGTTCTGTAATTAAGAGACGGGATTGGATTGGTCAATCTGCTGTTTACTGAATATCTTACTCCATATATGAAGTTCCGTTACCATAAACTTTAATTTTCATAACTCTTCTGTTTATACATGCAACTCTTTCATTAATTTCTCTCTCTGTCTTCATTTAGTTCTTGTGTTCTAGTAAATCTGATAAATTCTTCTTCCTCCACTGAGTCAAACAAACATTTGAGAGAATCCCACCTGGGAATTATGTTATCTCATTAGAACACTAGCTAATTTTTGACTTCCCATTTGAATATCATATACTTGATCTTCAAATTTGATATTGGATTCAGGAAGTTCCAGGGAGAAGGGAATGGAGAAATGGTTGGCAAGCTCAGATCTCAGAATTGAAGAGAAGAAGCATGGACAGGGATTCAAGACAAAATATGTCATGGGGAGCTCGAATGCGCCGGCAAATGAATGAACAGGTCTGATGGAATTATATATATCAAGGGAAAATTATGTTTGACTCAACATCAGTACATAGAAAAACGGCCTATTTTGGTAACAATAATTTTGATGTTAGTTCTTCCCCCCTTTCCCTTTTGTAATTTGCTTAAGACGTTACTCTATGTAAAAACTAACAATTACTAGAAAACAAATCTAATTCATAGAGTTAGAAAATAAAAGCCAAAGTTGTGCATATGGTCTCCAACTTTAGTTTTCTGAACCAAGGTAGGGAATAATTAAAGGAAACCGTCAAATATCTGCCATTTTGGTCCTGTAATTTAATTTTTATTCTATTTTGGGCCCTTTTACTTTCATCCAACTTTAGTCTATTGAAAA

mRNA sequence

GTACAGATGACTCAGCCTTGGACGTCCCGTATAAATAGCAGAAGATGCAGAGAACGGAATATAGACGGAGAAAAACAGAGAAATGCAGTGCTCTGATTTTACCTTTTCAGGGAGCGATTCACGCTTCTACATCCCTTCGAACCCTAACATTACCGGTCGCCGACCAATTCCCTGCCACCGATCTCGGGTTTTCTTTCCCAGCACTCCGCCGTGCAATTCGACGCGCTCGCCGCCTCTTTCAATCAGAGCCAAGGCCTCGTTCAACGAAGGAACCGTCTCCTCCGAGGTCGCCGAAGGCTCCTTCTACGATTTGCTCGGTATATCTCAGTCCGGATCCTTGGAGGAAATCAAGCACGCGTACAAGCAACTCGCTCGGAAGTACCATCCGGACGTGTCGCCGCCGGGGCGTGCGGAGGAGTATACGAAGAGGTTCATTCGTGTTCAGGAGGCGTACGAGACATTGTCGGATCCGAGGAGGAGAGCGCTGTATGATAGAGATATGGTTGGAGGTCTTCAAGTCGCATTCTCTGGTCGGAGACGATACGACGCCCATGAGGAAGTTCCAGGGAGAAGGGAATGGAGAAATGGTTGGCAAGCTCAGATCTCAGAATTGAAGAGAAGAAGCATGGACAGGGATTCAAGACAAAATATGTCATGGGGAGCTCGAATGCGCCGGCAAATGAATGAACAGGTCTGATGGAATTATATATATCAAGGGAAAATTATGTTTGACTCAACATCAGTACATAGAAAAACGGCCTATTTTGGTAACAATAATTTTGATGTTAGTTCTTCCCCCCTTTCCCTTTTGTAATTTGCTTAAGACGTTACTCTATGTAAAAACTAACAATTACTAGAAAACAAATCTAATTCATAGAGTTAGAAAATAAAAGCCAAAGTTGTGCATATGGTCTCCAACTTTAGTTTTCTGAACCAAGGTAGGGAATAATTAAAGGAAACCGTCAAATATCTGCCATTTTGGTCCTGTAATTTAATTTTTATTCTATTTTGGGCCCTTTTACTTTCATCCAACTTTAGTCTATTGAAAA

Coding sequence (CDS)

ATGCAGTGCTCTGATTTTACCTTTTCAGGGAGCGATTCACGCTTCTACATCCCTTCGAACCCTAACATTACCGGTCGCCGACCAATTCCCTGCCACCGATCTCGGGTTTTCTTTCCCAGCACTCCGCCGTGCAATTCGACGCGCTCGCCGCCTCTTTCAATCAGAGCCAAGGCCTCGTTCAACGAAGGAACCGTCTCCTCCGAGGTCGCCGAAGGCTCCTTCTACGATTTGCTCGGTATATCTCAGTCCGGATCCTTGGAGGAAATCAAGCACGCGTACAAGCAACTCGCTCGGAAGTACCATCCGGACGTGTCGCCGCCGGGGCGTGCGGAGGAGTATACGAAGAGGTTCATTCGTGTTCAGGAGGCGTACGAGACATTGTCGGATCCGAGGAGGAGAGCGCTGTATGATAGAGATATGGTTGGAGGTCTTCAAGTCGCATTCTCTGGTCGGAGACGATACGACGCCCATGAGGAAGTTCCAGGGAGAAGGGAATGGAGAAATGGTTGGCAAGCTCAGATCTCAGAATTGAAGAGAAGAAGCATGGACAGGGATTCAAGACAAAATATGTCATGGGGAGCTCGAATGCGCCGGCAAATGAATGAACAGGTCTGA

Protein sequence

MQCSDFTFSGSDSRFYIPSNPNITGRRPIPCHRSRVFFPSTPPCNSTRSPPLSIRAKASFNEGTVSSEVAEGSFYDLLGISQSGSLEEIKHAYKQLARKYHPDVSPPGRAEEYTKRFIRVQEAYETLSDPRRRALYDRDMVGGLQVAFSGRRRYDAHEEVPGRREWRNGWQAQISELKRRSMDRDSRQNMSWGARMRRQMNEQV
Homology
[Close]
BLAST of Bhi01G000145 vs. TAIR10
Match: AT4G13830.2 (DNAJ-like 20)

HSP 1 Score: 164.5 bits (415), Expect = 7.0e-41
Identity = 95/169 (56.21%), Postives = 118/169 (69.82%), Query Frame = 0 

Query: 36  VFFPSTPPCNSTRSPPLSIRAKASFNEGTVSSEVAEGSFYDLLGISQSGSLEEIKHAYKQ 95
           + +P+    +STR     I+++ + ++    SE  + SFYDLLG+++S +L EIK AYKQ
Sbjct: 36  ISYPTRTRFSSTR-----IQSRLTHDDPVKQSE--DLSFYDLLGVTESVTLPEIKQAYKQ 95

Query: 96  LARKYHPDVSPPGRAEEYTKRFIRVQEAYETLSDPRRRALYDRDMVGGLQVAFSGRR--R 155
           LARKYHPDVSPP R EEYT RFIRVQEAYETLSDPRRR LYDRD+  G   +FSGRR  R
Sbjct: 96  LARKYHPDVSPPDRVEEYTDRFIRVQEAYETLSDPRRRVLYDRDLSMGFSFSFSGRRQNR 155

Query: 156 YDAHEEVPGRREWRNGWQAQISELKRRSMDRDSRQNMSWGARMRRQMNE 203
           YD  E V  + EW+  WQ Q+S L+RRS  +D+   MSW ARMRRQ  E
Sbjct: 156 YD-QEVVEEKSEWKAKWQTQLSGLRRRSNQKDN-NTMSWAARMRRQQQE 195

[Close]
BLAST of Bhi01G000145 vs. TAIR10
Match: AT2G22360.1 (DNAJ heat shock family protein)

HSP 1 Score: 69.7 bits (169), Expect = 2.4e-12
Identity = 35/78 (44.87%), Postives = 51/78 (65.38%), Query Frame = 0 

Query: 70  AEGSFYDLLGISQSGSLEEIKHAYKQLARKYHPDVSPPGRAEEYTKRFIRVQEAYETLSD 129
           A+  +Y +LG+S++ +  EIK AY++LAR YHPDV+    AEE   +F  +  AYE LSD
Sbjct: 83  ADADYYSVLGVSKNATKAEIKSAYRKLARNYHPDVNKDPGAEE---KFKEISNAYEVLSD 142

Query: 130 PRRRALYDRDMVGGLQVA 148
             +++LYDR    GL+ A
Sbjct: 143 DEKKSLYDRYGEAGLKGA 157

[Close]
BLAST of Bhi01G000145 vs. TAIR10
Match: AT1G80030.1 (Molecular chaperone Hsp40/DnaJ family protein)

HSP 1 Score: 68.9 bits (167), Expect = 4.0e-12
Identity = 35/79 (44.30%), Postives = 52/79 (65.82%), Query Frame = 0 

Query: 72  GSFYDLLGISQSGSLEEIKHAYKQLARKYHPDVSPPGRAEEYTKRFIRVQEAYETLSDPR 131
           G +Y  LG+S+S + +EIK AY++LAR+YHPDV+    A   T++F  +  AYE LSD +
Sbjct: 74  GDYYATLGVSKSANNKEIKAAYRRLARQYHPDVNKEPGA---TEKFKEISAAYEVLSDEQ 133

Query: 132 RRALYDRDMVGGLQVAFSG 151
           +RALYD+    G++    G
Sbjct: 134 KRALYDQYGEAGVKSTVGG 149

[Close]
BLAST of Bhi01G000145 vs. TAIR10
Match: AT4G39960.1 (Molecular chaperone Hsp40/DnaJ family protein)

HSP 1 Score: 68.2 bits (165), Expect = 6.8e-12
Identity = 33/69 (47.83%), Postives = 47/69 (68.12%), Query Frame = 0 

Query: 70  AEGSFYDLLGISQSGSLEEIKHAYKQLARKYHPDVSPPGRAEEYTKRFIRVQEAYETLSD 129
           A+  FY +LG+S++ +  EIK AY++LAR YHPDV+    AE+   +F  +  AYE LSD
Sbjct: 82  ADTDFYSVLGVSKNATKAEIKSAYRKLARSYHPDVNKDAGAED---KFKEISNAYEILSD 141

Query: 130 PRRRALYDR 139
             +R+LYDR
Sbjct: 142 DEKRSLYDR 147

[Close]
BLAST of Bhi01G000145 vs. TAIR10
Match: AT3G17830.1 (Molecular chaperone Hsp40/DnaJ family protein)

HSP 1 Score: 65.5 bits (158), Expect = 4.4e-11
Identity = 47/135 (34.81%), Postives = 71/135 (52.59%), Query Frame = 0 

Query: 17  IPS-NPNITGRRPIPCHRSRVFFPSTPPCNSTRSPPLSIRAKASFNEGTVSSEVAEGSFY 76
           IPS NP  + R   P   +  + P T P  S R     I   +      V +  A    Y
Sbjct: 10  IPSINPAHSFRHQFPQPNASFYLPPTLPIFS-RVRRFGI---SGGYRRRVITMAAGTDHY 69

Query: 77  DLLGISQSGSLEEIKHAYKQLARKYHPDVSPPGRAEEYTKRFIRVQEAYETLSDPRRRAL 136
             L ++++ +L+EIK +Y++LARKYHPD++    AE+   +F ++  AYE LSD  +R+ 
Sbjct: 70  STLNVNRNATLQEIKSSYRKLARKYHPDMNKNPGAED---KFKQISAAYEVLSDEEKRSA 129

Query: 137 YDRDMVGGLQVAFSG 151
           YDR    GL+  F+G
Sbjct: 130 YDRFGEAGLEGDFNG 137

[Close]
BLAST of Bhi01G000145 vs. Swiss-Prot
Match: sp|Q9SDN0|DNJ20_ARATH (Chaperone protein dnaJ 20, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=ATJ20 PE=1 SV=2)

HSP 1 Score: 164.5 bits (415), Expect = 1.3e-39
Identity = 95/169 (56.21%), Postives = 118/169 (69.82%), Query Frame = 0 

Query: 36  VFFPSTPPCNSTRSPPLSIRAKASFNEGTVSSEVAEGSFYDLLGISQSGSLEEIKHAYKQ 95
           + +P+    +STR     I+++ + ++    SE  + SFYDLLG+++S +L EIK AYKQ
Sbjct: 36  ISYPTRTRFSSTR-----IQSRLTHDDPVKQSE--DLSFYDLLGVTESVTLPEIKQAYKQ 95

Query: 96  LARKYHPDVSPPGRAEEYTKRFIRVQEAYETLSDPRRRALYDRDMVGGLQVAFSGRR--R 155
           LARKYHPDVSPP R EEYT RFIRVQEAYETLSDPRRR LYDRD+  G   +FSGRR  R
Sbjct: 96  LARKYHPDVSPPDRVEEYTDRFIRVQEAYETLSDPRRRVLYDRDLSMGFSFSFSGRRQNR 155

Query: 156 YDAHEEVPGRREWRNGWQAQISELKRRSMDRDSRQNMSWGARMRRQMNE 203
           YD  E V  + EW+  WQ Q+S L+RRS  +D+   MSW ARMRRQ  E
Sbjct: 156 YD-QEVVEEKSEWKAKWQTQLSGLRRRSNQKDN-NTMSWAARMRRQQQE 195

[Close]
BLAST of Bhi01G000145 vs. Swiss-Prot
Match: sp|A7GT07|DNAJ_BACCN (Chaperone protein DnaJ OS=Bacillus cytotoxicus (strain DSM 22905 / CIP 110041 / 391-98 / NVH 391-98) OX=315749 GN=dnaJ PE=3 SV=1)

HSP 1 Score: 76.3 bits (186), Expect = 4.5e-13
Identity = 38/82 (46.34%), Postives = 56/82 (68.29%), Query Frame = 0 

Query: 69  VAEGSFYDLLGISQSGSLEEIKHAYKQLARKYHPDVSPPGRAEEYTKRFIRVQEAYETLS 128
           +++  +Y++LG+S+S S +EIK AY++LA+KYHPDVS   + E   ++F  VQEAYE LS
Sbjct: 1   MSKRDYYEVLGVSKSASKDEIKKAYRRLAKKYHPDVS---KEENAVEKFKEVQEAYEVLS 60

Query: 129 DPRRRALYDRDMVGGLQVAFSG 151
           D ++RA YD+    G    F G
Sbjct: 61  DEQKRAQYDQFGHAGSSQGFGG 79

[Close]
BLAST of Bhi01G000145 vs. Swiss-Prot
Match: sp|Q8E7Q7|DNAJ_STRA3 (Chaperone protein DnaJ OS=Streptococcus agalactiae serotype III (strain NEM316) OX=211110 GN=dnaJ PE=3 SV=1)

HSP 1 Score: 76.3 bits (186), Expect = 4.5e-13
Identity = 37/77 (48.05%), Postives = 52/77 (67.53%), Query Frame = 0 

Query: 74  FYDLLGISQSGSLEEIKHAYKQLARKYHPDVSPPGRAEEYTKRFIRVQEAYETLSDPRRR 133
           FYD LG+S+  S +EIK AY+++++KYHPD++    AEE   ++  VQEAYETLSD ++R
Sbjct: 6   FYDRLGVSKDASQDEIKKAYRRMSKKYHPDINKETGAEE---KYKEVQEAYETLSDTQKR 65

Query: 134 ALYDRDMVGGLQVAFSG 151
           A YD+    G    F G
Sbjct: 66  AAYDQYGAAGANGGFGG 79

[Close]
BLAST of Bhi01G000145 vs. Swiss-Prot
Match: sp|Q8E298|DNAJ_STRA5 (Chaperone protein DnaJ OS=Streptococcus agalactiae serotype V (strain ATCC BAA-611 / 2603 V/R) OX=208435 GN=dnaJ PE=3 SV=1)

HSP 1 Score: 76.3 bits (186), Expect = 4.5e-13
Identity = 37/77 (48.05%), Postives = 52/77 (67.53%), Query Frame = 0 

Query: 74  FYDLLGISQSGSLEEIKHAYKQLARKYHPDVSPPGRAEEYTKRFIRVQEAYETLSDPRRR 133
           FYD LG+S+  S +EIK AY+++++KYHPD++    AEE   ++  VQEAYETLSD ++R
Sbjct: 6   FYDRLGVSKDASQDEIKKAYRRMSKKYHPDINKETGAEE---KYKEVQEAYETLSDTQKR 65

Query: 134 ALYDRDMVGGLQVAFSG 151
           A YD+    G    F G
Sbjct: 66  AAYDQYGAAGANGGFGG 79

[Close]
BLAST of Bhi01G000145 vs. Swiss-Prot
Match: sp|B7IFE0|DNAJ_THEAB (Chaperone protein DnaJ OS=Thermosipho africanus (strain TCF52B) OX=484019 GN=dnaJ PE=3 SV=1)

HSP 1 Score: 75.9 bits (185), Expect = 5.9e-13
Identity = 35/70 (50.00%), Postives = 53/70 (75.71%), Query Frame = 0 

Query: 69  VAEGSFYDLLGISQSGSLEEIKHAYKQLARKYHPDVSPPGRAEEYTKRFIRVQEAYETLS 128
           +A+  +Y++LG+S++ S EEI+ AYKQL +K+HPD +   R +E  ++F  +QEAYE LS
Sbjct: 1   MAKKDYYEILGVSRNASQEEIRQAYKQLIKKWHPDRNHQNR-KEAEEKFKEIQEAYEVLS 60

Query: 129 DPRRRALYDR 139
           DP +RA+YDR
Sbjct: 61  DPEKRAMYDR 69

[Close]
BLAST of Bhi01G000145 vs. TrEMBL
Match: tr|A0A1S3AWS2|A0A1S3AWS2_CUCME (chaperone protein dnaJ 20, chloroplastic-like OS=Cucumis melo OX=3656 GN=LOC103483465 PE=4 SV=1)

HSP 1 Score: 345.1 bits (884), Expect = 1.1e-91
Identity = 175/204 (85.78%), Postives = 183/204 (89.71%), Query Frame = 0 

Query: 1   MQCSDFTFSGSDSRFYIPSNPNITGRRPIPCHRSRVFFPSTPPCNSTRSPPLSIRAKASF 60
           MQCSDFTFSGSDSRFYIPSNP I+ RRPI  +RSRVFFP TPP NSTR P LSIRAKASF
Sbjct: 27  MQCSDFTFSGSDSRFYIPSNPTISARRPISGYRSRVFFPHTPPFNSTRLPSLSIRAKASF 86

Query: 61  NEGTVSSEVAEGSFYDLLGISQSGSLEEIKHAYKQLARKYHPDVSPPGRAEEYTKRFIRV 120
           NEG VSSEVAEGSFYDLLGISQSGSL EIK AYKQLARKYHPDVSPPG AEEYTK FIRV
Sbjct: 87  NEGFVSSEVAEGSFYDLLGISQSGSLAEIKRAYKQLARKYHPDVSPPGGAEEYTKMFIRV 146

Query: 121 QEAYETLSDPRRRALYDRDMVGGLQVAFSGRRRYDAHEEVPGRREWRNGWQAQISELKRR 180
           QEAYETLSDPRRRALYDRDM+GGLQVAFS RRRYDA EEV  +  WRN W+AQISELKRR
Sbjct: 147 QEAYETLSDPRRRALYDRDMIGGLQVAFSARRRYDAAEEVAEKSGWRNSWEAQISELKRR 206

Query: 181 SMDRDSRQNMSWGARMRRQMNEQV 205
           SM++D RQNMSWGARMRRQMNEQ+
Sbjct: 207 SMEKDLRQNMSWGARMRRQMNEQI 230

[Close]
BLAST of Bhi01G000145 vs. TrEMBL
Match: tr|A0A0A0L4L9|A0A0A0L4L9_CUCSA (Chaperone protein dnaJ 20, chloroplast OS=Cucumis sativus OX=3659 GN=Csa_3G129700 PE=4 SV=1)

HSP 1 Score: 332.4 bits (851), Expect = 7.1e-88
Identity = 170/203 (83.74%), Postives = 178/203 (87.68%), Query Frame = 0 

Query: 1   MQCSDFTFSGSDSRFYIPSNPNITGRRPIPCHRSRVFFPSTPPCNSTRSPPLSIRAKASF 60
           MQCSD TFSGSDSRFYIPSNP  T RRPI  +RSRV FP TPP NSTR P LSIRAKASF
Sbjct: 14  MQCSDLTFSGSDSRFYIPSNPTTTARRPISGYRSRVCFPHTPPFNSTRLPSLSIRAKASF 73

Query: 61  NEGTVSSEVAEGSFYDLLGISQSGSLEEIKHAYKQLARKYHPDVSPPGRAEEYTKRFIRV 120
           NEG VSSEVAEGSFYDLLGIS+SGSLEEIK AYKQLARKYHPDVSPPG  EE TKRFIRV
Sbjct: 74  NEGFVSSEVAEGSFYDLLGISKSGSLEEIKRAYKQLARKYHPDVSPPGCVEENTKRFIRV 133

Query: 121 QEAYETLSDPRRRALYDRDMVGGLQVAFSGRRRYDAHEEVPGRREWRNGWQAQISELKRR 180
           QEAYETL+DPRRRALYDRDM+GGLQVAFS RRRYDA EEV  +  WRN W+AQISELKRR
Sbjct: 134 QEAYETLADPRRRALYDRDMIGGLQVAFSARRRYDADEEVAQKSGWRNSWEAQISELKRR 193

Query: 181 SMDRDSRQNMSWGARMRRQMNEQ 204
           SM++D R NMSWGARMRRQMNEQ
Sbjct: 194 SMEKDLRPNMSWGARMRRQMNEQ 216

[Close]
BLAST of Bhi01G000145 vs. TrEMBL
Match: tr|A0A2P5A3X2|A0A2P5A3X2_PARAD (Terminal organelle assembly protein OS=Parasponia andersonii OX=3476 GN=PanWU01x14_371400 PE=4 SV=1)

HSP 1 Score: 217.2 bits (552), Expect = 3.3e-53
Identity = 121/209 (57.89%), Postives = 149/209 (71.29%), Query Frame = 0 

Query: 1   MQCSDFTFSGSDSRFYIP--SNPNITGRRPIPCHRSRVFF----PSTPPCNSTRSPPLSI 60
           M+C   T SGSDSRF +P  S P  T R P P + S +FF    P        R  P+  
Sbjct: 1   MRCHGLTISGSDSRFLVPQYSTPISTPRPPNP-NLSPLFFSVRIPKPVYSARIRVGPVKA 60

Query: 61  RAKASFNEGTVS-SEVAEGSFYDLLGISQSGSLEEIKHAYKQLARKYHPDVSPPGRAEEY 120
           +A ++ ++G V+ +  AE SFY+LLGI +SGSL +IK AYKQLARKYHPDVSPP R EEY
Sbjct: 61  KAASAVDDGLVAEAAAAELSFYELLGIPESGSLVQIKQAYKQLARKYHPDVSPPDRVEEY 120

Query: 121 TKRFIRVQEAYETLSDPRRRALYDRDMVGGLQVAFSGRRRYDAHEEVPGRREWRNGWQAQ 180
           T+RFIRVQEAYETLSDPRRRA+YDRDM  GL +AFS RRRY   E +  + EW+  WQ+Q
Sbjct: 121 TRRFIRVQEAYETLSDPRRRAMYDRDMAKGLHLAFSARRRYQNDEGMEEKSEWKTRWQSQ 180

Query: 181 ISELKRRSMDRDSRQNMSWGARMRRQMNE 203
           +SELKRRSM++D R NMSWGARMRR+ ++
Sbjct: 181 LSELKRRSMNKDVRGNMSWGARMRRRRDD 208

[Close]
BLAST of Bhi01G000145 vs. TrEMBL
Match: tr|A0A2P5C875|A0A2P5C875_9ROSA (Terminal organelle assembly protein OS=Trema orientalis OX=63057 GN=TorRG33x02_294110 PE=4 SV=1)

HSP 1 Score: 213.4 bits (542), Expect = 4.8e-52
Identity = 120/209 (57.42%), Postives = 147/209 (70.33%), Query Frame = 0 

Query: 1   MQCSDFTFSGSDSRFYIPSN--PNITGRRPIPCHRSRVFF----PSTPPCNSTRSPPLSI 60
           M+C   T  GSDSRF I  N  P  T R P P + S +FF    P        R  P+  
Sbjct: 1   MRCYGLTIPGSDSRFLISQNSTPVSTSRPPNP-NLSPLFFTVRIPKPVYSTRIRVGPVKA 60

Query: 61  RAKASFNEGTVS-SEVAEGSFYDLLGISQSGSLEEIKHAYKQLARKYHPDVSPPGRAEEY 120
           +A ++ ++G V+ +  AE SFY+LLGI +SGSL EIK AYKQLARKYHPDVSPP R EEY
Sbjct: 61  KAASAVDDGFVAEAAAAELSFYELLGIPESGSLVEIKQAYKQLARKYHPDVSPPDRVEEY 120

Query: 121 TKRFIRVQEAYETLSDPRRRALYDRDMVGGLQVAFSGRRRYDAHEEVPGRREWRNGWQAQ 180
           T+RFIRVQEAYETLSDPRRRA+YDRDM  GL +AFS RRR+   E +  + EW+N WQ+Q
Sbjct: 121 TRRFIRVQEAYETLSDPRRRAMYDRDMAKGLHLAFSARRRHQNDEGLEEKSEWKNRWQSQ 180

Query: 181 ISELKRRSMDRDSRQNMSWGARMRRQMNE 203
           +SELKRRSM++D   NMSWGARMRR+ ++
Sbjct: 181 VSELKRRSMNKDVGGNMSWGARMRRRRDD 208

[Close]
BLAST of Bhi01G000145 vs. TrEMBL
Match: tr|A0A1S3C500|A0A1S3C500_CUCME (chaperone protein dnaJ 20, chloroplastic-like OS=Cucumis melo OX=3656 GN=LOC103496947 PE=4 SV=1)

HSP 1 Score: 211.5 bits (537), Expect = 1.8e-51
Identity = 113/202 (55.94%), Postives = 139/202 (68.81%), Query Frame = 0 

Query: 1   MQCSDFTFSGSDSRFYIPSNPNITGRRPIPCHRSRVFFPSTPPCNSTRSPPLSIRAKASF 60
           M+      SG+DSR Y P+ P I    P     S+V F  +P     R P   +R KA+F
Sbjct: 1   MRSCGLALSGTDSRCYFPATPPIFS--PRRASNSQVSFLKSP-----RPPSACVRVKAAF 60

Query: 61  NEGTVSSEVAEGSFYDLLGISQSGSLEEIKHAYKQLARKYHPDVSPPGRAEEYTKRFIRV 120
               V S+  E SFY+LLG+ +SGS+ EIK AYKQ+A KYHPDVSPPGR EEYT++FI++
Sbjct: 61  INDAVVSDAVEVSFYELLGVPESGSMLEIKQAYKQMALKYHPDVSPPGRIEEYTRKFIKL 120

Query: 121 QEAYETLSDPRRRALYDRDMVGGLQVAFSGRRRYDAHEEVPGRREWRNGWQAQISELKRR 180
           QEAYETLSDP  RALYDRD+  GLQ AFS R+R   HEE+  +  WRN WQ Q+S LKRR
Sbjct: 121 QEAYETLSDPNMRALYDRDLARGLQFAFSSRKRCYDHEEMEEKSGWRNRWQDQLSGLKRR 180

Query: 181 SMDRDSRQNMSWGARMRRQMNE 203
           SM+RDS+ NMSWGARMRR+ +E
Sbjct: 181 SMNRDSKANMSWGARMRRERDE 195

[Close]
BLAST of Bhi01G000145 vs. NCBI nr
Match: XP_008438327.1 (PREDICTED: chaperone protein dnaJ 20, chloroplastic-like [Cucumis melo])

HSP 1 Score: 345.1 bits (884), Expect = 1.6e-91
Identity = 175/204 (85.78%), Postives = 183/204 (89.71%), Query Frame = 0 

Query: 1   MQCSDFTFSGSDSRFYIPSNPNITGRRPIPCHRSRVFFPSTPPCNSTRSPPLSIRAKASF 60
           MQCSDFTFSGSDSRFYIPSNP I+ RRPI  +RSRVFFP TPP NSTR P LSIRAKASF
Sbjct: 27  MQCSDFTFSGSDSRFYIPSNPTISARRPISGYRSRVFFPHTPPFNSTRLPSLSIRAKASF 86

Query: 61  NEGTVSSEVAEGSFYDLLGISQSGSLEEIKHAYKQLARKYHPDVSPPGRAEEYTKRFIRV 120
           NEG VSSEVAEGSFYDLLGISQSGSL EIK AYKQLARKYHPDVSPPG AEEYTK FIRV
Sbjct: 87  NEGFVSSEVAEGSFYDLLGISQSGSLAEIKRAYKQLARKYHPDVSPPGGAEEYTKMFIRV 146

Query: 121 QEAYETLSDPRRRALYDRDMVGGLQVAFSGRRRYDAHEEVPGRREWRNGWQAQISELKRR 180
           QEAYETLSDPRRRALYDRDM+GGLQVAFS RRRYDA EEV  +  WRN W+AQISELKRR
Sbjct: 147 QEAYETLSDPRRRALYDRDMIGGLQVAFSARRRYDAAEEVAEKSGWRNSWEAQISELKRR 206

Query: 181 SMDRDSRQNMSWGARMRRQMNEQV 205
           SM++D RQNMSWGARMRRQMNEQ+
Sbjct: 207 SMEKDLRQNMSWGARMRRQMNEQI 230

[Close]
BLAST of Bhi01G000145 vs. NCBI nr
Match: XP_023526392.1 (chaperone protein dnaJ 20, chloroplastic-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 333.2 bits (853), Expect = 6.3e-88
Identity = 169/204 (82.84%), Postives = 182/204 (89.22%), Query Frame = 0 

Query: 1   MQCSDFTFSGSDSRFYIPSNPNITGRRPIPCHRSRVFFPSTPPCNSTRSPPLSIRAKASF 60
           MQCSDFTFSGSDSRFYIPS+P+ITGRRPI  HRS +FFP+T PCN  R P LSIRAKASF
Sbjct: 1   MQCSDFTFSGSDSRFYIPSSPSITGRRPITGHRSGIFFPNTLPCNLMRLPSLSIRAKASF 60

Query: 61  NEGTVSSEVAEGSFYDLLGISQSGSLEEIKHAYKQLARKYHPDVSPPGRAEEYTKRFIRV 120
           NEGTVSSEVA+GSFYDLLG+S+S SL EIK AYKQLARKYHPDVSP GRAEEYTKRFIRV
Sbjct: 61  NEGTVSSEVADGSFYDLLGVSESVSLAEIKRAYKQLARKYHPDVSPIGRAEEYTKRFIRV 120

Query: 121 QEAYETLSDPRRRALYDRDMVGGLQVAFSGRRRYDAHEEVPGRREWRNGWQAQISELKRR 180
           QEAYETLSDPRRRALYDRDMVGGLQVAFS  R YDA +E P +  WR+ WQ+QISELKRR
Sbjct: 121 QEAYETLSDPRRRALYDRDMVGGLQVAFSAWRLYDADQEAPEKSGWRSCWQSQISELKRR 180

Query: 181 SMDRDSRQNMSWGARMRRQMNEQV 205
           SM++DS QNMSWGARMRRQMNEQV
Sbjct: 181 SMNKDSSQNMSWGARMRRQMNEQV 204

[Close]
BLAST of Bhi01G000145 vs. NCBI nr
Match: XP_004133982.1 (PREDICTED: chaperone protein dnaJ 20, chloroplastic-like [Cucumis sativus] >KGN56713.1 Chaperone protein dnaJ 20, chloroplast [Cucumis sativus])

HSP 1 Score: 332.4 bits (851), Expect = 1.1e-87
Identity = 170/203 (83.74%), Postives = 178/203 (87.68%), Query Frame = 0 

Query: 1   MQCSDFTFSGSDSRFYIPSNPNITGRRPIPCHRSRVFFPSTPPCNSTRSPPLSIRAKASF 60
           MQCSD TFSGSDSRFYIPSNP  T RRPI  +RSRV FP TPP NSTR P LSIRAKASF
Sbjct: 14  MQCSDLTFSGSDSRFYIPSNPTTTARRPISGYRSRVCFPHTPPFNSTRLPSLSIRAKASF 73

Query: 61  NEGTVSSEVAEGSFYDLLGISQSGSLEEIKHAYKQLARKYHPDVSPPGRAEEYTKRFIRV 120
           NEG VSSEVAEGSFYDLLGIS+SGSLEEIK AYKQLARKYHPDVSPPG  EE TKRFIRV
Sbjct: 74  NEGFVSSEVAEGSFYDLLGISKSGSLEEIKRAYKQLARKYHPDVSPPGCVEENTKRFIRV 133

Query: 121 QEAYETLSDPRRRALYDRDMVGGLQVAFSGRRRYDAHEEVPGRREWRNGWQAQISELKRR 180
           QEAYETL+DPRRRALYDRDM+GGLQVAFS RRRYDA EEV  +  WRN W+AQISELKRR
Sbjct: 134 QEAYETLADPRRRALYDRDMIGGLQVAFSARRRYDADEEVAQKSGWRNSWEAQISELKRR 193

Query: 181 SMDRDSRQNMSWGARMRRQMNEQ 204
           SM++D R NMSWGARMRRQMNEQ
Sbjct: 194 SMEKDLRPNMSWGARMRRQMNEQ 216

[Close]
BLAST of Bhi01G000145 vs. NCBI nr
Match: XP_022974737.1 (chaperone protein dnaJ 20, chloroplastic-like [Cucurbita maxima])

HSP 1 Score: 326.2 bits (835), Expect = 7.7e-86
Identity = 163/204 (79.90%), Postives = 178/204 (87.25%), Query Frame = 0 

Query: 1   MQCSDFTFSGSDSRFYIPSNPNITGRRPIPCHRSRVFFPSTPPCNSTRSPPLSIRAKASF 60
           MQCSDFT+SG D R  IPS+P+ITGRR I   +SRVFFP+TPPCNSTR P LSIRAKASF
Sbjct: 1   MQCSDFTYSGGDYRILIPSSPSITGRRQISGQKSRVFFPNTPPCNSTRLPSLSIRAKASF 60

Query: 61  NEGTVSSEVAEGSFYDLLGISQSGSLEEIKHAYKQLARKYHPDVSPPGRAEEYTKRFIRV 120
           N G  S E A+GSFYDLLGIS+SGSL EIK AYKQLARKYHPDVSPPGRAEEYTKRFIRV
Sbjct: 61  NGGIASFEAADGSFYDLLGISESGSLAEIKRAYKQLARKYHPDVSPPGRAEEYTKRFIRV 120

Query: 121 QEAYETLSDPRRRALYDRDMVGGLQVAFSGRRRYDAHEEVPGRREWRNGWQAQISELKRR 180
           QEAYETLSDPRRRALYDRDMVGGLQVAFS RRRYD  E VP +  W++ W+AQISELKRR
Sbjct: 121 QEAYETLSDPRRRALYDRDMVGGLQVAFSARRRYDVDEGVPDKSAWKSCWKAQISELKRR 180

Query: 181 SMDRDSRQNMSWGARMRRQMNEQV 205
           SMD+DS+ N+SWGARMR+QMNEQV
Sbjct: 181 SMDKDSKHNLSWGARMRQQMNEQV 204

[Close]
BLAST of Bhi01G000145 vs. NCBI nr
Match: XP_022949677.1 (chaperone protein dnaJ 20, chloroplastic-like [Cucurbita moschata])

HSP 1 Score: 325.1 bits (832), Expect = 1.7e-85
Identity = 163/204 (79.90%), Postives = 178/204 (87.25%), Query Frame = 0 

Query: 1   MQCSDFTFSGSDSRFYIPSNPNITGRRPIPCHRSRVFFPSTPPCNSTRSPPLSIRAKASF 60
           MQCSDFT+S  D R  IPS+P+ITGRR I   RSRVFFP+TPPCNSTR P LSIRAKASF
Sbjct: 35  MQCSDFTYSVGDYRILIPSSPSITGRRQISGQRSRVFFPNTPPCNSTRLPSLSIRAKASF 94

Query: 61  NEGTVSSEVAEGSFYDLLGISQSGSLEEIKHAYKQLARKYHPDVSPPGRAEEYTKRFIRV 120
           N G  SSE A+GSFYDLLGIS+SGSL EIK AYKQLARKYHPDVSPPGRAEEYTKRFIRV
Sbjct: 95  NGGIASSEAADGSFYDLLGISESGSLAEIKRAYKQLARKYHPDVSPPGRAEEYTKRFIRV 154

Query: 121 QEAYETLSDPRRRALYDRDMVGGLQVAFSGRRRYDAHEEVPGRREWRNGWQAQISELKRR 180
           QEAYETLSDPRRRALYDRDMVGGLQVAFS RRRYD  E VP +  W++ W+AQISELK+R
Sbjct: 155 QEAYETLSDPRRRALYDRDMVGGLQVAFSARRRYDVDEGVPDKSAWKSCWKAQISELKKR 214

Query: 181 SMDRDSRQNMSWGARMRRQMNEQV 205
           SMD+DS+ N+SWGARMR+QMNEQV
Sbjct: 215 SMDKDSKHNLSWGARMRQQMNEQV 238

The following BLAST results are available for this feature:
BLAST of Bhi01G000145 vs. TAIR10
Match NameE-valueIdentityDescription
AT4G13830.27.0e-4156.21DNAJ-like 20[more]
AT2G22360.12.4e-1244.87DNAJ heat shock family protein[more]
AT1G80030.14.0e-1244.30Molecular chaperone Hsp40/DnaJ family protein[more]
AT4G39960.16.8e-1247.83Molecular chaperone Hsp40/DnaJ family protein[more]
AT3G17830.14.4e-1134.81Molecular chaperone Hsp40/DnaJ family protein[more]
BLAST of Bhi01G000145 vs. Swiss-Prot
Match NameE-valueIdentityDescription
sp|Q9SDN0|DNJ20_ARATH1.3e-3956.21Chaperone protein dnaJ 20, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=ATJ2... [more]
sp|A7GT07|DNAJ_BACCN4.5e-1346.34Chaperone protein DnaJ OS=Bacillus cytotoxicus (strain DSM 22905 / CIP 110041 / ... [more]
sp|Q8E7Q7|DNAJ_STRA34.5e-1348.05Chaperone protein DnaJ OS=Streptococcus agalactiae serotype III (strain NEM316) ... [more]
sp|Q8E298|DNAJ_STRA54.5e-1348.05Chaperone protein DnaJ OS=Streptococcus agalactiae serotype V (strain ATCC BAA-6... [more]
sp|B7IFE0|DNAJ_THEAB5.9e-1350.00Chaperone protein DnaJ OS=Thermosipho africanus (strain TCF52B) OX=484019 GN=dna... [more]
BLAST of Bhi01G000145 vs. TrEMBL
Match NameE-valueIdentityDescription
tr|A0A1S3AWS2|A0A1S3AWS2_CUCME1.1e-9185.78chaperone protein dnaJ 20, chloroplastic-like OS=Cucumis melo OX=3656 GN=LOC1034... [more]
tr|A0A0A0L4L9|A0A0A0L4L9_CUCSA7.1e-8883.74Chaperone protein dnaJ 20, chloroplast OS=Cucumis sativus OX=3659 GN=Csa_3G12970... [more]
tr|A0A2P5A3X2|A0A2P5A3X2_PARAD3.3e-5357.89Terminal organelle assembly protein OS=Parasponia andersonii OX=3476 GN=PanWU01x... [more]
tr|A0A2P5C875|A0A2P5C875_9ROSA4.8e-5257.42Terminal organelle assembly protein OS=Trema orientalis OX=63057 GN=TorRG33x02_2... [more]
tr|A0A1S3C500|A0A1S3C500_CUCME1.8e-5155.94chaperone protein dnaJ 20, chloroplastic-like OS=Cucumis melo OX=3656 GN=LOC1034... [more]
BLAST of Bhi01G000145 vs. NCBI nr
Match NameE-valueIdentityDescription
XP_008438327.11.6e-9185.78PREDICTED: chaperone protein dnaJ 20, chloroplastic-like [Cucumis melo][more]
XP_023526392.16.3e-8882.84chaperone protein dnaJ 20, chloroplastic-like [Cucurbita pepo subsp. pepo][more]
XP_004133982.11.1e-8783.74PREDICTED: chaperone protein dnaJ 20, chloroplastic-like [Cucumis sativus] >KGN5... [more]
XP_022974737.17.7e-8679.90chaperone protein dnaJ 20, chloroplastic-like [Cucurbita maxima][more]
XP_022949677.11.7e-8579.90chaperone protein dnaJ 20, chloroplastic-like [Cucurbita moschata][more]
Annotated Terms
The following terms have been associated with this gene:
Vocabulary: INTERPRO
TermDefinition
IPR018253DnaJ_domain_CS
IPR036869J_dom_sf
IPR001623DnaJ_domain
GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0061077 chaperone-mediated protein folding
biological_process GO:0044238 primary metabolic process
biological_process GO:0009408 response to heat
biological_process GO:0006457 protein folding
biological_process GO:0008150 biological_process
biological_process GO:0019222 regulation of metabolic process
biological_process GO:0071704 organic substance metabolic process
biological_process GO:0044237 cellular metabolic process
biological_process GO:0008152 metabolic process
cellular_component GO:0009507 chloroplast
cellular_component GO:0043231 intracellular membrane-bounded organelle
cellular_component GO:0005575 cellular_component
molecular_function GO:0003674 molecular_function
molecular_function GO:0005524 ATP binding
molecular_function GO:0031072 heat shock protein binding
molecular_function GO:0046872 metal ion binding
molecular_function GO:0051082 unfolded protein binding
Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Bhi01M000145Bhi01M000145mRNA


InterPro
Analysis Name: InterPro Annotations of wax gourd
Date Performed: 2019-11-17
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001623DnaJ domainPRINTSPR00625JDOMAINcoord: 75..93
score: 44.99
coord: 132..151
score: 25.5
coord: 112..132
score: 39.13
coord: 93..108
score: 48.68
IPR001623DnaJ domainSMARTSM00271dnaj_3coord: 72..132
e-value: 7.2E-21
score: 85.3
IPR001623DnaJ domainPFAMPF00226DnaJcoord: 74..137
e-value: 6.4E-21
score: 74.2
IPR001623DnaJ domainPROSITEPS50076DNAJ_2coord: 73..140
score: 19.901
IPR001623DnaJ domainCDDcd06257DnaJcoord: 74..129
e-value: 5.04976E-20
score: 79.5117
IPR036869Chaperone J-domain superfamilyGENE3DG3DSA:1.10.287.110coord: 61..157
e-value: 2.6E-23
score: 84.0
IPR036869Chaperone J-domain superfamilySUPERFAMILYSSF46565Chaperone J-domaincoord: 73..149
NoneNo IPR availablePANTHERPTHR45090FAMILY NOT NAMEDcoord: 30..203
IPR018253DnaJ domain, conserved sitePROSITEPS00636DNAJ_1coord: 117..136

Synteny
The following gene(s) are orthologous to this gene:
GeneOrthologueOrganismBlock
Bhi01G000145Csa3G129700Cucumber (Chinese Long) v2cuwgoB270
Bhi01G000145MELO3C006295Melon (DHL92) v3.5.1mewgoB531
Bhi01G000145ClCG05G003140Watermelon (Charleston Gray)wcgwgoB428
Bhi01G000145CSPI03G10650Wild cucumber (PI 183967)cpiwgoB270
Bhi01G000145Cucsa.256640Cucumber (Gy14) v1cgywgoB523
Bhi01G000145CmaCh06G012000Cucurbita maxima (Rimu)cmawgoB1007
Bhi01G000145CmaCh14G019070Cucurbita maxima (Rimu)cmawgoB0329
Bhi01G000145CmoCh14G019620Cucurbita moschata (Rifu)cmowgoB0318
Bhi01G000145CmoCh06G012370Cucurbita moschata (Rifu)cmowgoB1006
Bhi01G000145Lsi05G018600Bottle gourd (USVL1VR-Ls)lsiwgoB445
Bhi01G000145Cp4.1LG03g18080Cucurbita pepo (Zucchini)cpewgoB0784
Bhi01G000145Cp4.1LG08g02660Cucurbita pepo (Zucchini)cpewgoB1111
Bhi01G000145Cp4.1LG12g00650Cucurbita pepo (Zucchini)cpewgoB0192
Bhi01G000145CsGy3G010700Cucumber (Gy14) v2cgybwgoB258
Bhi01G000145MELO3C006295.2Melon (DHL92) v3.6.1medwgoB532
Bhi01G000145Carg06307Silver-seed gourdcarwgoB1118
Bhi01G000145Carg24775Silver-seed gourdcarwgoB0224
Bhi01G000145Carg10152Silver-seed gourdcarwgoB0285
Bhi01G000145CsaV3_3G010760Cucumber (Chinese Long) v3cucwgoB273
Bhi01G000145Cla021401Watermelon (97103) v1wgowmB203
Bhi01G000145Cla97C05G083770Watermelon (97103) v2wgowmbB180
The following gene(s) are paralogous to this gene:

None

The following block(s) are covering this gene:
GeneOrganismBlock
Bhi01G000145Cucurbita maxima (Rimu)cmawgoB0475
Bhi01G000145Cucurbita moschata (Rifu)cmowgoB0474
Bhi01G000145Bottle gourd (USVL1VR-Ls)lsiwgoB043
Bhi01G000145Watermelon (Charleston Gray)wcgwgoB525
Bhi01G000145Watermelon (97103) v2wgowmbB206