Lsi05G018600 (gene) Bottle gourd (USVL1VR-Ls) | Cucurbit Genomics Database (CuGenDB)

Overview


Name	Lsi05G018600
Type	gene
Organism	Lagenaria siceraria (Bottle gourd (USVL1VR-Ls))
Description	Chaperone protein dnaJ 20
Location	chr05 : 25829776 .. 25831793 (+)

Genome Viewer

Gene Expression

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

TTTTAATTCTAAACCGTACAAGTGACTCAGCCTTGGACGTCCCGTATAAATGTAGAAGATCCAGAGAACGGAAGATAGACGGAGAAAAACAGAGAAATGCAGTGCTCCGATTTTACCTTATCAGGGAGCGATTCACGCTTCTACATCCCTTCGAACCCTAGCACTACCGGCCGCCGACCAATTTCCGGCCACCGATCTCGGATATTCTTTCCCAACACCCTGCCGTGCAATTCGACGCGCTTGCCGTCTCTTTCAATCAGAGCCAAGGCCTCGTACAACGAAGGAATCCTCTCCTCCGAGGTCGCCGAAGGCTCCTTCTACGATTTGCTCGGTATATCTCAGTCCGGATCCTTGGCGGAAATCAAGCACGCGTACAAGCAGCTCGCTCGGAAGTACCATCCGGACGTGTCGCCGCCGGGCCGTGCGGAGGAGTATACGAAGACGTTCATTCGTGTTCAGGAGGCGTACGAGACATTGTCGGATCCGAGGAGGAGAGCGCTGTATGATAGAGATATGGTTGGAGGCCTTCAAGTCGCGTTCTCTGCTCGGAGACGATACGACGCCGATGAGGTATTAGTTCAGTCTACCAATTTTAACTTTGTGTTTTTTTCGTTATTTTAGGGGTAATAATTATGTTTGATTGTTTTTTCGTTTTTTTAGGGGTAATTATGTTTGATTGTTAACAGATTTTGAAATCTCTCGAGTTTCTTTTGCGTTTGCCCTTATTTTTGAAGTGATTTCGTGTTTAATTTCTCAGATGTTGTACAGAGAGATTAGAATGAGTGAAGGATTCGATCTTAATGTTTCTTTGATTTTGGATTTTTGGAGTGACGAAACGAAACGTCGTTTCATTCTGGGTCCTGATTCTCTTTTTCTTTCCTTTTTTGTTCGTCTGACTCTATCCTATACGGATGCAGTAGGATTGGTGGAGACGGATCCTGTTTCTGTTTAACAAGGACCTACAATTGGTTAATCAGTGATTTGTTCTGTAATTAAGAAACGGAATGGGATTGGTCAATCTGCTGTTTTCTGGATATCTTACCCCAAATATGGAATTCCGTTACCATAAACTTTAATTTTCATTACTCTTCTGTTTATCTATATGCAACTTTTTCACATAATATTTCTTTCTGTCTTCATTTAATTGTTGTATTCTAGCAAATCTGTATGAAATCCTTTTTCACCCACTGATTCAAACAAAACATTTGAGTTAATCCCACCTGGGAGTCCTGTTATCTCATTAGAACACATGCCAATTTTTTACTTCCCATTTGAATATCATACACTAAATCTCCAAATTTGATTTTGAATTCAGGAAGTTCCAGAGAGAAGTGGATGGAAAAATTGCTGGCAAGCTCAGATCTCAGAATTGAAGAGAAGAAGCATGGACAAGGATTCGAGACAAAATATGTCATGGGGAGCTCGAATGCGCCGGCAAATGAATGAACAGGTCTGATTGAATTATATAGATTAAGAAAACCATGTTTGACTTTCAGCATCAGTAAATATAATACGGCCTATTTAGTAACAATAATTTTGTTGTTAGTTTTCTCAATTTCCCCTTTGTAATCTGTGCTATACTATATATAAAAAGGGTTATGAGTGTAAAATCTTTGTTTCCCATCTTAGACCTCACAATTTTTCATAAATCCTACTTTGTCTTTCGTCACAACGTGTCGAGGTGATGGTTATTCTTTTATGTACCCGTGTAAGCTAGACCATATTTGAGTAGTACATTATGATCTAACAGCCCATCCAACTAGGATTGAACTCATGACCTCTTAGCCCTTTGTCAGGATCATTCCTCCTATCTAATAAGCCGACCAATGATAGTTTACAAAGAAAATCTAATAAGTTAAAAAATCAGAACCAAATTTGTAATTATATGGTCCCCAATTTTAGTTTTTTGAACTGAGACACAAAAAAATTGAAAGAGACCGTCAAGAGAGTGTTTAAGACGTCGAGTTGAGTTTTGAAATATGAAGTTAATATGATGGAGTTAGGTAGTTTGTGTTTGG

mRNA sequence

TTTTAATTCTAAACCGTACAAGTGACTCAGCCTTGGACGTCCCGTATAAATGTAGAAGATCCAGAGAACGGAAGATAGACGGAGAAAAACAGAGAAATGCAGTGCTCCGATTTTACCTTATCAGGGAGCGATTCACGCTTCTACATCCCTTCGAACCCTAGCACTACCGGCCGCCGACCAATTTCCGGCCACCGATCTCGGATATTCTTTCCCAACACCCTGCCGTGCAATTCGACGCGCTTGCCGTCTCTTTCAATCAGAGCCAAGGCCTCGTACAACGAAGGAATCCTCTCCTCCGAGGTCGCCGAAGGCTCCTTCTACGATTTGCTCGGTATATCTCAGTCCGGATCCTTGGCGGAAATCAAGCACGCGTACAAGCAGCTCGCTCGGAAGTACCATCCGGACGTGTCGCCGCCGGGCCGTGCGGAGGAGTATACGAAGACGTTCATTCGTGTTCAGGAGGCGTACGAGACATTGTCGGATCCGAGGAGGAGAGCGCTGTATGATAGAGATATGGTTGGAGGCCTTCAAGTCGCGTTCTCTGCTCGGAGACGATACGACGCCGATGAGGAAGTTCCAGAGAGAAGTGGATGGAAAAATTGCTGGCAAGCTCAGATCTCAGAATTGAAGAGAAGAAGCATGGACAAGGATTCGAGACAAAATATGTCATGGGGAGCTCGAATGCGCCGGCAAATGAATGAACAGGTCTGATTGAATTATATAGATTAAGAAAACCATGTTTGACTTTCAGCATCAGTAAATATAATACGGCCTATTTAGTAACAATAATTTTGTTGTTAGTTTTCTCAATTTCCCCTTTGTAATCTGTGCTATACTATATATAAAAAGGGTTATGAGTGTAAAATCTTTGTTTCCCATCTTAGACCTCACAATTTTTCATAAATCCTACTTTGTCTTTCGTCACAACGTGTCGAGGTGATGGTTATTCTTTTATGTACCCGTGTAAGCTAGACCATATTTGAGTAGTACATTATGATCTAACAGCCCATCCAACTAGGATTGAACTCATGACCTCTTAGCCCTTTGTCAGGATCATTCCTCCTATCTAATAAGCCGACCAATGATAGTTTACAAAGAAAATCTAATAAGTTAAAAAATCAGAACCAAATTTGTAATTATATGGTCCCCAATTTTAGTTTTTTGAACTGAGACACAAAAAAATTGAAAGAGACCGTCAAGAGAGTGTTTAAGACGTCGAGTTGAGTTTTGAAATATGAAGTTAATATGATGGAGTTAGGTAGTTTGTGTTTGG

Coding sequence (CDS)

ATGCAGTGCTCCGATTTTACCTTATCAGGGAGCGATTCACGCTTCTACATCCCTTCGAACCCTAGCACTACCGGCCGCCGACCAATTTCCGGCCACCGATCTCGGATATTCTTTCCCAACACCCTGCCGTGCAATTCGACGCGCTTGCCGTCTCTTTCAATCAGAGCCAAGGCCTCGTACAACGAAGGAATCCTCTCCTCCGAGGTCGCCGAAGGCTCCTTCTACGATTTGCTCGGTATATCTCAGTCCGGATCCTTGGCGGAAATCAAGCACGCGTACAAGCAGCTCGCTCGGAAGTACCATCCGGACGTGTCGCCGCCGGGCCGTGCGGAGGAGTATACGAAGACGTTCATTCGTGTTCAGGAGGCGTACGAGACATTGTCGGATCCGAGGAGGAGAGCGCTGTATGATAGAGATATGGTTGGAGGCCTTCAAGTCGCGTTCTCTGCTCGGAGACGATACGACGCCGATGAGGAAGTTCCAGAGAGAAGTGGATGGAAAAATTGCTGGCAAGCTCAGATCTCAGAATTGAAGAGAAGAAGCATGGACAAGGATTCGAGACAAAATATGTCATGGGGAGCTCGAATGCGCCGGCAAATGAATGAACAGGTCTGA

Protein sequence

MQCSDFTLSGSDSRFYIPSNPSTTGRRPISGHRSRIFFPNTLPCNSTRLPSLSIRAKASYNEGILSSEVAEGSFYDLLGISQSGSLAEIKHAYKQLARKYHPDVSPPGRAEEYTKTFIRVQEAYETLSDPRRRALYDRDMVGGLQVAFSARRRYDADEEVPERSGWKNCWQAQISELKRRSMDKDSRQNMSWGARMRRQMNEQV

Homology

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BLAST of Lsi05G018600 vs. Swiss-Prot
Match: DNJ20_ARATH (Chaperone protein dnaJ 20, chloroplastic OS=Arabidopsis thaliana GN=ATJ20 PE=1 SV=2)

HSP 1 Score: 171.8 bits (434), Expect = 7.8e-42
Identity = 95/158 (60.13%), Postives = 114/158 (72.15%), Query Frame = 1


Query: 47  TRLPSLSIRAKASYNEGILSSEVAEGSFYDLLGISQSGSLAEIKHAYKQLARKYHPDVSP 106
           TR  S  I+++ ++++ +  SE  + SFYDLLG+++S +L EIK AYKQLARKYHPDVSP
Sbjct: 42  TRFSSTRIQSRLTHDDPVKQSE--DLSFYDLLGVTESVTLPEIKQAYKQLARKYHPDVSP 101

Query: 107 PGRAEEYTKTFIRVQEAYETLSDPRRRALYDRDMVGGLQVAFSARR--RYDADEEVPERS 166
           P R EEYT  FIRVQEAYETLSDPRRR LYDRD+  G   +FS RR  RYD  E V E+S
Sbjct: 102 PDRVEEYTDRFIRVQEAYETLSDPRRRVLYDRDLSMGFSFSFSGRRQNRYD-QEVVEEKS 161

Query: 167 GWKNCWQAQISELKRRSMDKDSRQNMSWGARMRRQMNE 203
            WK  WQ Q+S L+RRS  KD+   MSW ARMRRQ  E
Sbjct: 162 EWKAKWQTQLSGLRRRSNQKDN-NTMSWAARMRRQQQE 195

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BLAST of Lsi05G018600 vs. Swiss-Prot
Match: DNAJ_THEAB (Chaperone protein DnaJ OS=Thermosipho africanus (strain TCF52B) GN=dnaJ PE=3 SV=1)

HSP 1 Score: 73.6 bits (179), Expect = 2.9e-12
Identity = 35/70 (50.00%), Postives = 50/70 (71.43%), Query Frame = 1


Query: 69  VAEGSFYDLLGISQSGSLAEIKHAYKQLARKYHPDVSPPGRAEEYTKTFIRVQEAYETLS 128
           +A+  +Y++LG+S++ S  EI+ AYKQL +K+HPD +   R E   K F  +QEAYE LS
Sbjct: 1   MAKKDYYEILGVSRNASQEEIRQAYKQLIKKWHPDRNHQNRKEAEEK-FKEIQEAYEVLS 60

Query: 129 DPRRRALYDR 139
           DP +RA+YDR
Sbjct: 61  DPEKRAMYDR 69

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BLAST of Lsi05G018600 vs. Swiss-Prot
Match: DNAJ_LACRD (Chaperone protein DnaJ OS=Lactobacillus reuteri (strain DSM 20016) GN=dnaJ PE=3 SV=1)

HSP 1 Score: 73.2 bits (178), Expect = 3.8e-12
Identity = 39/79 (49.37%), Postives = 53/79 (67.09%), Query Frame = 1


Query: 69  VAEGSFYDLLGISQSGSLAEIKHAYKQLARKYHPDVSPPGRAEEYTKTFIRVQEAYETLS 128
           +AE  +YD+LG+S+  S  +IK AY++LA KYHPDV+    AEE    F ++ EAYETLS
Sbjct: 1   MAEQDYYDILGVSKDASEKDIKRAYRRLAAKYHPDVNHEPGAEE---KFKKINEAYETLS 60

Query: 129 DPRRRALYDRDMVGGLQVA 148
           D ++RA YD+    G Q A
Sbjct: 61  DSQKRAQYDQFGSAGPQGA 76

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BLAST of Lsi05G018600 vs. Swiss-Prot
Match: DNAJ_LACRJ (Chaperone protein DnaJ OS=Lactobacillus reuteri (strain JCM 1112) GN=dnaJ PE=3 SV=1)

HSP 1 Score: 73.2 bits (178), Expect = 3.8e-12
Identity = 39/79 (49.37%), Postives = 53/79 (67.09%), Query Frame = 1


Query: 69  VAEGSFYDLLGISQSGSLAEIKHAYKQLARKYHPDVSPPGRAEEYTKTFIRVQEAYETLS 128
           +AE  +YD+LG+S+  S  +IK AY++LA KYHPDV+    AEE    F ++ EAYETLS
Sbjct: 1   MAEQDYYDILGVSKDASEKDIKRAYRRLAAKYHPDVNHEPGAEE---KFKKINEAYETLS 60

Query: 129 DPRRRALYDRDMVGGLQVA 148
           D ++RA YD+    G Q A
Sbjct: 61  DSQKRAQYDQFGSAGPQGA 76

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BLAST of Lsi05G018600 vs. Swiss-Prot
Match: DNAJ_LACF3 (Chaperone protein DnaJ OS=Lactobacillus fermentum (strain NBRC 3956 / LMG 18251) GN=dnaJ PE=3 SV=1)

HSP 1 Score: 73.2 bits (178), Expect = 3.8e-12
Identity = 39/77 (50.65%), Postives = 51/77 (66.23%), Query Frame = 1


Query: 69  VAEGSFYDLLGISQSGSLAEIKHAYKQLARKYHPDVSPPGRAEEYTKTFIRVQEAYETLS 128
           +AE   YD+LG+ +  S AEIK AY++LA KYHPDV+    AE   K F ++ EAYETLS
Sbjct: 1   MAEEDLYDVLGVKKDASEAEIKRAYRKLAAKYHPDVNHEPGAE---KKFKKINEAYETLS 60

Query: 129 DPRRRALYDRDMVGGLQ 146
           D ++RA YD+    G Q
Sbjct: 61  DDQKRAQYDQFGTTGSQ 74

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BLAST of Lsi05G018600 vs. TrEMBL
Match: A0A0A0L4L9_CUCSA (Chaperone protein dnaJ 20, chloroplast OS=Cucumis sativus GN=Csa_3G129700 PE=4 SV=1)

HSP 1 Score: 346.3 bits (887), Expect = 2.6e-92
Identity = 172/203 (84.73%), Postives = 186/203 (91.63%), Query Frame = 1


Query: 1   MQCSDFTLSGSDSRFYIPSNPSTTGRRPISGHRSRIFFPNTLPCNSTRLPSLSIRAKASY 60
           MQCSD T SGSDSRFYIPSNP+TT RRPISG+RSR+ FP+T P NSTRLPSLSIRAKAS+
Sbjct: 14  MQCSDLTFSGSDSRFYIPSNPTTTARRPISGYRSRVCFPHTPPFNSTRLPSLSIRAKASF 73

Query: 61  NEGILSSEVAEGSFYDLLGISQSGSLAEIKHAYKQLARKYHPDVSPPGRAEEYTKTFIRV 120
           NEG +SSEVAEGSFYDLLGIS+SGSL EIK AYKQLARKYHPDVSPPG  EE TK FIRV
Sbjct: 74  NEGFVSSEVAEGSFYDLLGISKSGSLEEIKRAYKQLARKYHPDVSPPGCVEENTKRFIRV 133

Query: 121 QEAYETLSDPRRRALYDRDMVGGLQVAFSARRRYDADEEVPERSGWKNCWQAQISELKRR 180
           QEAYETL+DPRRRALYDRDM+GGLQVAFSARRRYDADEEV ++SGW+N W+AQISELKRR
Sbjct: 134 QEAYETLADPRRRALYDRDMIGGLQVAFSARRRYDADEEVAQKSGWRNSWEAQISELKRR 193

Query: 181 SMDKDSRQNMSWGARMRRQMNEQ 204
           SM+KD R NMSWGARMRRQMNEQ
Sbjct: 194 SMEKDLRPNMSWGARMRRQMNEQ 216

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BLAST of Lsi05G018600 vs. TrEMBL
Match: W9S291_9ROSA (Chaperone protein dnaJ 20 OS=Morus notabilis GN=L484_011075 PE=4 SV=1)

HSP 1 Score: 236.1 bits (601), Expect = 3.7e-59
Identity = 125/203 (61.58%), Postives = 151/203 (74.38%), Query Frame = 1


Query: 1   MQCSDFTLS-GSDSRFYIPSNPSTTGRRPISGHRSRIFFPNTLPCNSTRLPSLSIRAKAS 60
           M+C+   +S GSDSRF+ P+N ST          S + FP   P  STR+ +     +AS
Sbjct: 1   MRCNGLAISGGSDSRFFFPTNSSTIYTASARNPSSTVLFPGR-PVFSTRIRA---TPRAS 60

Query: 61  YNEGILSSEVAEGSFYDLLGISQSGSLAEIKHAYKQLARKYHPDVSPPGRAEEYTKTFIR 120
               ++   VAE SFY+LLGI +SGSL EIK AYKQLARKYHPDVSPPGR EEYT+TFIR
Sbjct: 61  AAAAVVGDRVAELSFYELLGIPESGSLGEIKQAYKQLARKYHPDVSPPGRVEEYTRTFIR 120

Query: 121 VQEAYETLSDPRRRALYDRDMVGGLQVAFSARRRYDADEEVPERSGWKNCWQAQISELKR 180
           VQEAYETLSDPR RA+YDRDM  GL +AFSARRRY +DE + E+S WK+ WQ+Q+SELKR
Sbjct: 121 VQEAYETLSDPRLRAIYDRDMAKGLHLAFSARRRYQSDEGMEEKSEWKSHWQSQLSELKR 180

Query: 181 RSMDKDSRQNMSWGARMRRQMNE 203
           RS++KDSR NMSWGARMRR+M +
Sbjct: 181 RSVNKDSRGNMSWGARMRRRMED 199

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BLAST of Lsi05G018600 vs. TrEMBL
Match: A0A061EJK6_THECC (Chaperone protein dnaJ 20 OS=Theobroma cacao GN=TCM_020242 PE=4 SV=1)

HSP 1 Score: 215.3 bits (547), Expect = 6.8e-53
Identity = 115/200 (57.50%), Postives = 140/200 (70.00%), Query Frame = 1


Query: 3   CSDFTLSGSDSRFYIPSNPSTTGRRPISGHRSRIFFPNTLPCNSTRLPSLSIRAKASYNE 62
           C    + G+D+RF++P       RRP+               + TR+   S++ KA  NE
Sbjct: 5   CGLAAIPGTDARFFVPDTSFIRLRRPV---------------DPTRVQFGSLKIKARINE 64

Query: 63  GILSSEVAEGSFYDLLGISQSGSLAEIKHAYKQLARKYHPDVSPPGRAEEYTKTFIRVQE 122
                   E SFY+LLGI ++G+L EIK AYKQLARKYHPDVSPP R EEYT+ FIRVQE
Sbjct: 65  AR-----KEVSFYELLGIPETGTLPEIKQAYKQLARKYHPDVSPPDRVEEYTQRFIRVQE 124

Query: 123 AYETLSDPRRRALYDRDMVGGLQVAFSARRRYDADEEVPERSGWKNCWQAQISELKRRSM 182
           AYETLSDPRRRA YDRD+  G+ +AFSARRRY  DEE+ ERS WKN WQ+Q+SELKRRSM
Sbjct: 125 AYETLSDPRRRASYDRDLALGIHLAFSARRRYQYDEELEERSEWKNRWQSQLSELKRRSM 184

Query: 183 DKDSRQNMSWGARMRRQMNE 203
           ++D+R NMSWGARMRRQ +E
Sbjct: 185 NRDARGNMSWGARMRRQRDE 184

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BLAST of Lsi05G018600 vs. TrEMBL
Match: B9RWC0_RICCO (Chaperone protein dnaJ 20, chloroplast, putative OS=Ricinus communis GN=RCOM_1017840 PE=4 SV=1)

HSP 1 Score: 211.8 bits (538), Expect = 7.6e-52
Identity = 114/199 (57.29%), Postives = 138/199 (69.35%), Query Frame = 1


Query: 1   MQCSDFTLSGSDSRFYIPSNPSTTGRRPISGHRSRIFFPNTLPCNSTRLPSLSIRAKASY 60
           M C++  L G     Y     S++   PI+  +   F     P   TR P  S + KA+ 
Sbjct: 1   MHCTNLMLPGGSDTVYFFHPSSSSSTTPITKSKPGPFL-RLKPV--TRFPMSSFKTKAAV 60

Query: 61  NEGILSSEVAEGSFYDLLGISQSGSLAEIKHAYKQLARKYHPDVSPPGRAEEYTKTFIRV 120
           ++ I + ++   SFY+LLGI +SGSL +IK AYKQLARKYHPDVSPP R  EYT+ FI+V
Sbjct: 61  SDRIATKDL---SFYELLGIPESGSLIDIKQAYKQLARKYHPDVSPPDRVHEYTQRFIQV 120

Query: 121 QEAYETLSDPRRRALYDRDMVGGLQVAFSARRRYDADEEVPERSGWKNCWQAQISELKRR 180
           QEAYETLSDPRRRA YDRDM  GL +AFSARRRY +DEEV   S WKN W+AQ+SELKRR
Sbjct: 121 QEAYETLSDPRRRATYDRDMARGLHLAFSARRRYQSDEEVEVSSDWKNRWEAQLSELKRR 180

Query: 181 SMDKDSRQNMSWGARMRRQ 200
           SM+KDS +NMSW ARMRRQ
Sbjct: 181 SMNKDSEENMSWAARMRRQ 193

[Close]

BLAST of Lsi05G018600 vs. TrEMBL
Match: A0A059B1U7_EUCGR (Uncharacterized protein OS=Eucalyptus grandis GN=EUGRSUZ_H02689 PE=4 SV=1)

HSP 1 Score: 209.5 bits (532), Expect = 3.8e-51
Identity = 116/203 (57.14%), Postives = 138/203 (67.98%), Query Frame = 1


Query: 1   MQCSDFTLSGSD-SRFYIPSNPSTTGRRPISGHRSRIFFPNTLPCNSTRLPSLSIRAKAS 60
           M+C    L G D SR  +P  P         GHR+  F P  +   + R  +  I   A 
Sbjct: 1   MRCYGVALPGGDPSRLCLPPPPPPFPSARTHGHRA--FLPTRVRFGAVRCAAGRIDDGAV 60

Query: 61  YNEGILSSEVAEGSFYDLLGISQSGSLAEIKHAYKQLARKYHPDVSPPGRAEEYTKTFIR 120
            +    S+  AE SFYDLLGI +SG+L EIK AYKQLARKYHPDVSPPGR EEYT+ FIR
Sbjct: 61  ADAARCSA--AEASFYDLLGIPESGTLPEIKQAYKQLARKYHPDVSPPGRVEEYTQRFIR 120

Query: 121 VQEAYETLSDPRRRALYDRDMVGGLQVAFSARRRYDADEEVPERSGWKNCWQAQISELKR 180
           VQEAYETLSDPRRRA+YDRD+  G+ +AFSARR Y  DE++ ++S WK+ WQ Q+S LKR
Sbjct: 121 VQEAYETLSDPRRRAVYDRDLARGIHLAFSARRPYRGDEDLEDKSEWKSRWQVQLSGLKR 180

Query: 181 RSMDKDSRQNMSWGARMRRQMNE 203
           RS  KDSR NMSWGARMR+QM E
Sbjct: 181 RSAHKDSRGNMSWGARMRKQMEE 199

[Close]

BLAST of Lsi05G018600 vs. TAIR10
Match: AT4G13830.2 (AT4G13830.2 DNAJ-like 20)

HSP 1 Score: 171.8 bits (434), Expect = 4.4e-43
Identity = 95/158 (60.13%), Postives = 114/158 (72.15%), Query Frame = 1


Query: 47  TRLPSLSIRAKASYNEGILSSEVAEGSFYDLLGISQSGSLAEIKHAYKQLARKYHPDVSP 106
           TR  S  I+++ ++++ +  SE  + SFYDLLG+++S +L EIK AYKQLARKYHPDVSP
Sbjct: 42  TRFSSTRIQSRLTHDDPVKQSE--DLSFYDLLGVTESVTLPEIKQAYKQLARKYHPDVSP 101

Query: 107 PGRAEEYTKTFIRVQEAYETLSDPRRRALYDRDMVGGLQVAFSARR--RYDADEEVPERS 166
           P R EEYT  FIRVQEAYETLSDPRRR LYDRD+  G   +FS RR  RYD  E V E+S
Sbjct: 102 PDRVEEYTDRFIRVQEAYETLSDPRRRVLYDRDLSMGFSFSFSGRRQNRYD-QEVVEEKS 161

Query: 167 GWKNCWQAQISELKRRSMDKDSRQNMSWGARMRRQMNE 203
            WK  WQ Q+S L+RRS  KD+   MSW ARMRRQ  E
Sbjct: 162 EWKAKWQTQLSGLRRRSNQKDN-NTMSWAARMRRQQQE 195

[Close]

BLAST of Lsi05G018600 vs. TAIR10
Match: AT4G39960.1 (AT4G39960.1 Molecular chaperone Hsp40/DnaJ family protein)

HSP 1 Score: 71.6 bits (174), Expect = 6.2e-13
Identity = 43/119 (36.13%), Postives = 63/119 (52.94%), Query Frame = 1


Query: 29  ISGHRSRIFFPNTLPCNSTRLPSLSIRAKASYNEGILSSEVAEGSFYDLLGISQSGSLAE 88
           +   RS +F   +LP     L    +        G   +  A+  FY +LG+S++ + AE
Sbjct: 45  LGASRSSMFAQGSLPF----LSLTGVSPNTHSRRGARFTVRADTDFYSVLGVSKNATKAE 104

Query: 89  IKHAYKQLARKYHPDVSPPGRAEEYTKTFIRVQEAYETLSDPRRRALYDRDMVGGLQVA 148
           IK AY++LAR YHPDV+    AE+    F  +  AYE LSD  +R+LYDR    G++ A
Sbjct: 105 IKSAYRKLARSYHPDVNKDAGAED---KFKEISNAYEILSDDEKRSLYDRYGEAGVKGA 156

[Close]

BLAST of Lsi05G018600 vs. TAIR10
Match: AT2G22360.1 (AT2G22360.1 DNAJ heat shock family protein)

HSP 1 Score: 70.5 bits (171), Expect = 1.4e-12
Identity = 36/78 (46.15%), Postives = 51/78 (65.38%), Query Frame = 1


Query: 70  AEGSFYDLLGISQSGSLAEIKHAYKQLARKYHPDVSPPGRAEEYTKTFIRVQEAYETLSD 129
           A+  +Y +LG+S++ + AEIK AY++LAR YHPDV+    AEE    F  +  AYE LSD
Sbjct: 83  ADADYYSVLGVSKNATKAEIKSAYRKLARNYHPDVNKDPGAEE---KFKEISNAYEVLSD 142

Query: 130 PRRRALYDRDMVGGLQVA 148
             +++LYDR    GL+ A
Sbjct: 143 DEKKSLYDRYGEAGLKGA 157

[Close]

BLAST of Lsi05G018600 vs. TAIR10
Match: AT3G17830.1 (AT3G17830.1 Molecular chaperone Hsp40/DnaJ family protein)

HSP 1 Score: 67.0 bits (162), Expect = 1.5e-11
Identity = 46/134 (34.33%), Postives = 71/134 (52.99%), Query Frame = 1


Query: 17  IPS-NPSTTGRRPISGHRSRIFFPNTLPCNSTRLPSLSIRAKASYNEGILSSEVAEGSFY 76
           IPS NP+ + R       +  + P TLP  S R+    I     Y   +++   A    Y
Sbjct: 10  IPSINPAHSFRHQFPQPNASFYLPPTLPIFS-RVRRFGI--SGGYRRRVITM-AAGTDHY 69

Query: 77  DLLGISQSGSLAEIKHAYKQLARKYHPDVSPPGRAEEYTKTFIRVQEAYETLSDPRRRAL 136
             L ++++ +L EIK +Y++LARKYHPD++    AE+    F ++  AYE LSD  +R+ 
Sbjct: 70  STLNVNRNATLQEIKSSYRKLARKYHPDMNKNPGAED---KFKQISAAYEVLSDEEKRSA 129

Query: 137 YDRDMVGGLQVAFS 150
           YDR    GL+  F+
Sbjct: 130 YDRFGEAGLEGDFN 136

[Close]

BLAST of Lsi05G018600 vs. TAIR10
Match: AT1G80030.1 (AT1G80030.1 Molecular chaperone Hsp40/DnaJ family protein)

HSP 1 Score: 65.5 bits (158), Expect = 4.4e-11
Identity = 34/68 (50.00%), Postives = 47/68 (69.12%), Query Frame = 1


Query: 72  GSFYDLLGISQSGSLAEIKHAYKQLARKYHPDVS-PPGRAEEYTKTFIRVQEAYETLSDP 131
           G +Y  LG+S+S +  EIK AY++LAR+YHPDV+  PG  E+    F  +  AYE LSD 
Sbjct: 74  GDYYATLGVSKSANNKEIKAAYRRLARQYHPDVNKEPGATEK----FKEISAAYEVLSDE 133

Query: 132 RRRALYDR 139
           ++RALYD+
Sbjct: 134 QKRALYDQ 137

[Close]

BLAST of Lsi05G018600 vs. NCBI nr
Match: gi|659075782|ref|XP_008438327.1| (PREDICTED: chaperone protein dnaJ 20, chloroplastic-like [Cucumis melo])

HSP 1 Score: 359.8 bits (922), Expect = 3.2e-96
Identity = 178/204 (87.25%), Postives = 191/204 (93.63%), Query Frame = 1


Query: 1   MQCSDFTLSGSDSRFYIPSNPSTTGRRPISGHRSRIFFPNTLPCNSTRLPSLSIRAKASY 60
           MQCSDFT SGSDSRFYIPSNP+ + RRPISG+RSR+FFP+T P NSTRLPSLSIRAKAS+
Sbjct: 27  MQCSDFTFSGSDSRFYIPSNPTISARRPISGYRSRVFFPHTPPFNSTRLPSLSIRAKASF 86

Query: 61  NEGILSSEVAEGSFYDLLGISQSGSLAEIKHAYKQLARKYHPDVSPPGRAEEYTKTFIRV 120
           NEG +SSEVAEGSFYDLLGISQSGSLAEIK AYKQLARKYHPDVSPPG AEEYTK FIRV
Sbjct: 87  NEGFVSSEVAEGSFYDLLGISQSGSLAEIKRAYKQLARKYHPDVSPPGGAEEYTKMFIRV 146

Query: 121 QEAYETLSDPRRRALYDRDMVGGLQVAFSARRRYDADEEVPERSGWKNCWQAQISELKRR 180
           QEAYETLSDPRRRALYDRDM+GGLQVAFSARRRYDA EEV E+SGW+N W+AQISELKRR
Sbjct: 147 QEAYETLSDPRRRALYDRDMIGGLQVAFSARRRYDAAEEVAEKSGWRNSWEAQISELKRR 206

Query: 181 SMDKDSRQNMSWGARMRRQMNEQV 205
           SM+KD RQNMSWGARMRRQMNEQ+
Sbjct: 207 SMEKDLRQNMSWGARMRRQMNEQI 230

[Close]

BLAST of Lsi05G018600 vs. NCBI nr
Match: gi|449432390|ref|XP_004133982.1| (PREDICTED: chaperone protein dnaJ 20, chloroplastic-like [Cucumis sativus])

HSP 1 Score: 346.3 bits (887), Expect = 3.7e-92
Identity = 172/203 (84.73%), Postives = 186/203 (91.63%), Query Frame = 1


Query: 1   MQCSDFTLSGSDSRFYIPSNPSTTGRRPISGHRSRIFFPNTLPCNSTRLPSLSIRAKASY 60
           MQCSD T SGSDSRFYIPSNP+TT RRPISG+RSR+ FP+T P NSTRLPSLSIRAKAS+
Sbjct: 14  MQCSDLTFSGSDSRFYIPSNPTTTARRPISGYRSRVCFPHTPPFNSTRLPSLSIRAKASF 73

Query: 61  NEGILSSEVAEGSFYDLLGISQSGSLAEIKHAYKQLARKYHPDVSPPGRAEEYTKTFIRV 120
           NEG +SSEVAEGSFYDLLGIS+SGSL EIK AYKQLARKYHPDVSPPG  EE TK FIRV
Sbjct: 74  NEGFVSSEVAEGSFYDLLGISKSGSLEEIKRAYKQLARKYHPDVSPPGCVEENTKRFIRV 133

Query: 121 QEAYETLSDPRRRALYDRDMVGGLQVAFSARRRYDADEEVPERSGWKNCWQAQISELKRR 180
           QEAYETL+DPRRRALYDRDM+GGLQVAFSARRRYDADEEV ++SGW+N W+AQISELKRR
Sbjct: 134 QEAYETLADPRRRALYDRDMIGGLQVAFSARRRYDADEEVAQKSGWRNSWEAQISELKRR 193

Query: 181 SMDKDSRQNMSWGARMRRQMNEQ 204
           SM+KD R NMSWGARMRRQMNEQ
Sbjct: 194 SMEKDLRPNMSWGARMRRQMNEQ 216

[Close]

BLAST of Lsi05G018600 vs. NCBI nr
Match: gi|703148387|ref|XP_010109333.1| (Chaperone protein dnaJ 20 [Morus notabilis])

HSP 1 Score: 236.1 bits (601), Expect = 5.4e-59
Identity = 125/203 (61.58%), Postives = 151/203 (74.38%), Query Frame = 1


Query: 1   MQCSDFTLS-GSDSRFYIPSNPSTTGRRPISGHRSRIFFPNTLPCNSTRLPSLSIRAKAS 60
           M+C+   +S GSDSRF+ P+N ST          S + FP   P  STR+ +     +AS
Sbjct: 1   MRCNGLAISGGSDSRFFFPTNSSTIYTASARNPSSTVLFPGR-PVFSTRIRA---TPRAS 60

Query: 61  YNEGILSSEVAEGSFYDLLGISQSGSLAEIKHAYKQLARKYHPDVSPPGRAEEYTKTFIR 120
               ++   VAE SFY+LLGI +SGSL EIK AYKQLARKYHPDVSPPGR EEYT+TFIR
Sbjct: 61  AAAAVVGDRVAELSFYELLGIPESGSLGEIKQAYKQLARKYHPDVSPPGRVEEYTRTFIR 120

Query: 121 VQEAYETLSDPRRRALYDRDMVGGLQVAFSARRRYDADEEVPERSGWKNCWQAQISELKR 180
           VQEAYETLSDPR RA+YDRDM  GL +AFSARRRY +DE + E+S WK+ WQ+Q+SELKR
Sbjct: 121 VQEAYETLSDPRLRAIYDRDMAKGLHLAFSARRRYQSDEGMEEKSEWKSHWQSQLSELKR 180

Query: 181 RSMDKDSRQNMSWGARMRRQMNE 203
           RS++KDSR NMSWGARMRR+M +
Sbjct: 181 RSVNKDSRGNMSWGARMRRRMED 199

[Close]

BLAST of Lsi05G018600 vs. NCBI nr
Match: gi|1009139653|ref|XP_015887231.1| (PREDICTED: chaperone protein dnaJ 20, chloroplastic [Ziziphus jujuba])

HSP 1 Score: 218.8 bits (556), Expect = 8.9e-54
Identity = 122/212 (57.55%), Postives = 144/212 (67.92%), Query Frame = 1


Query: 1   MQCSDFTLSGSDSRFYI----PSNPSTTGRR------PISGHRSRIFFPNTLPCNSTRLP 60
           M+    T+SGSDSRF+     P N S           P   HR  IF   T     TR  
Sbjct: 1   MRSHGLTISGSDSRFFSFPVKPVNKSIPDPNHNVLFFPAHHHRPIIFTTTT-----TRDS 60

Query: 61  SLSIRAKASYNEGILSSEVAEGSFYDLLGISQSGSLAEIKHAYKQLARKYHPDVSPPGRA 120
             ++R KA+ N+G + SE AE SFY+LLGI + GSL +IK AYKQLARKYHPDVSPPGR 
Sbjct: 61  IGTVRVKATVNDGFVFSEAAELSFYELLGIPEWGSLVDIKQAYKQLARKYHPDVSPPGRV 120

Query: 121 EEYTKTFIRVQEAYETLSDPRRRALYDRDMVGGLQVAFSARRRYDADEEVPERSGWKNCW 180
           EEYT+ FIRVQEAYETLSDPRRRALYDRDM  G+ +AFSAR+RY  DE + ++  WK  W
Sbjct: 121 EEYTQRFIRVQEAYETLSDPRRRALYDRDMAKGIHLAFSARKRYQKDEGMEDKGEWKYRW 180

Query: 181 QAQISELKRRSMDKDSRQNMSWGARMRRQMNE 203
           Q+Q+SELKRRS +KD   NMSWGARMRR+  E
Sbjct: 181 QSQLSELKRRSTNKDMTGNMSWGARMRRRREE 207

[Close]

BLAST of Lsi05G018600 vs. NCBI nr
Match: gi|470121999|ref|XP_004297043.1| (PREDICTED: chaperone protein dnaJ 20, chloroplastic-like [Fragaria vesca subsp. vesca])

HSP 1 Score: 216.1 bits (549), Expect = 5.8e-53
Identity = 120/199 (60.30%), Postives = 142/199 (71.36%), Query Frame = 1


Query: 1   MQCSDFTLSGSDSRFYIPSNPSTTGRRPISGHRSRIFFPNTLPCNSTRLPSLSIRAKASY 60
           MQ    T+ G+DSR YI      +  RP    +     P++LP  S+R    SI+AKA+ 
Sbjct: 1   MQTYGLTIPGADSRLYITP---ISAARPAPDPK-----PSSLPVFSSRTRFGSIKAKAAT 60

Query: 61  NEGILSSEVAEGSFYDLLGISQSGSLAEIKHAYKQLARKYHPDVSPPGRAEEYTKTFIRV 120
             G +++E  E SFY+LLGI +SGS+ EIK AYKQLARKYHPDVSPPGR EEYT+ FIRV
Sbjct: 61  MNGAVTAEEKELSFYELLGIPESGSIKEIKQAYKQLARKYHPDVSPPGRVEEYTEKFIRV 120

Query: 121 QEAYETLSDPRRRALYDRDMVGGLQVAFSARRRYDADEEVPERSGWKNCWQAQISELKRR 180
           QEAYETLSD RRRALYD+DM  GL +AFSA RRY  DE +  +  WKN WQ+Q+SELKRR
Sbjct: 121 QEAYETLSDSRRRALYDQDMARGLHLAFSA-RRYQYDEGMEVKGDWKNRWQSQLSELKRR 180

Query: 181 SMDKDSRQNMSWGARMRRQ 200
           S  KD RQNMSWGARMRRQ
Sbjct: 181 SSYKDDRQNMSWGARMRRQ 190

The following BLAST results are available for this feature:

BLAST of Lsi05G018600 vs. Swiss-Prot


Match Name	E-value	Identity	Description
DNJ20_ARATH	7.8e-42	60.13	Chaperone protein dnaJ 20, chloroplastic OS=Arabidopsis thaliana GN=ATJ20 PE=1 S...	[more]
DNAJ_THEAB	2.9e-12	50.00	Chaperone protein DnaJ OS=Thermosipho africanus (strain TCF52B) GN=dnaJ PE=3 SV=...	[more]
DNAJ_LACRD	3.8e-12	49.37	Chaperone protein DnaJ OS=Lactobacillus reuteri (strain DSM 20016) GN=dnaJ PE=3 ...	[more]
DNAJ_LACRJ	3.8e-12	49.37	Chaperone protein DnaJ OS=Lactobacillus reuteri (strain JCM 1112) GN=dnaJ PE=3 S...	[more]
DNAJ_LACF3	3.8e-12	50.65	Chaperone protein DnaJ OS=Lactobacillus fermentum (strain NBRC 3956 / LMG 18251)...	[more]

BLAST of Lsi05G018600 vs. TrEMBL


Match Name	E-value	Identity	Description
A0A0A0L4L9_CUCSA	2.6e-92	84.73	Chaperone protein dnaJ 20, chloroplast OS=Cucumis sativus GN=Csa_3G129700 PE=4 S...	[more]
W9S291_9ROSA	3.7e-59	61.58	Chaperone protein dnaJ 20 OS=Morus notabilis GN=L484_011075 PE=4 SV=1	[more]
A0A061EJK6_THECC	6.8e-53	57.50	Chaperone protein dnaJ 20 OS=Theobroma cacao GN=TCM_020242 PE=4 SV=1	[more]
B9RWC0_RICCO	7.6e-52	57.29	Chaperone protein dnaJ 20, chloroplast, putative OS=Ricinus communis GN=RCOM_101...	[more]
A0A059B1U7_EUCGR	3.8e-51	57.14	Uncharacterized protein OS=Eucalyptus grandis GN=EUGRSUZ_H02689 PE=4 SV=1	[more]

BLAST of Lsi05G018600 vs. TAIR10


Match Name	E-value	Identity	Description
AT4G13830.2	4.4e-43	60.13	DNAJ-like 20	[more]
AT4G39960.1	6.2e-13	36.13	Molecular chaperone Hsp40/DnaJ family protein	[more]
AT2G22360.1	1.4e-12	46.15	DNAJ heat shock family protein	[more]
AT3G17830.1	1.5e-11	34.33	Molecular chaperone Hsp40/DnaJ family protein	[more]
AT1G80030.1	4.4e-11	50.00	Molecular chaperone Hsp40/DnaJ family protein	[more]

BLAST of Lsi05G018600 vs. NCBI nr


Match Name	E-value	Identity	Description
gi\|659075782\|ref\|XP_008438327.1\|	3.2e-96	87.25	PREDICTED: chaperone protein dnaJ 20, chloroplastic-like [Cucumis melo]	[more]
gi\|449432390\|ref\|XP_004133982.1\|	3.7e-92	84.73	PREDICTED: chaperone protein dnaJ 20, chloroplastic-like [Cucumis sativus]	[more]
gi\|703148387\|ref\|XP_010109333.1\|	5.4e-59	61.58	Chaperone protein dnaJ 20 [Morus notabilis]	[more]
gi\|1009139653\|ref\|XP_015887231.1\|	8.9e-54	57.55	PREDICTED: chaperone protein dnaJ 20, chloroplastic [Ziziphus jujuba]	[more]
gi\|470121999\|ref\|XP_004297043.1\|	5.8e-53	60.30	PREDICTED: chaperone protein dnaJ 20, chloroplastic-like [Fragaria vesca subsp. ...	[more]

Annotated Terms

The following terms have been associated with this gene:

Vocabulary: **INTERPRO**
Term	Definition
IPR018253	DnaJ_domain_CS
IPR001623	DnaJ_domain

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0008150	biological_process
biological_process	GO:0008152	metabolic process
biological_process	GO:0044237	cellular metabolic process
biological_process	GO:0071704	organic substance metabolic process
biological_process	GO:0044238	primary metabolic process
biological_process	GO:0019222	regulation of metabolic process
cellular_component	GO:0005575	cellular_component
cellular_component	GO:0043231	intracellular membrane-bounded organelle
molecular_function	GO:0003674	molecular_function

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lsi05G018600.1	Lsi05G018600.1	mRNA

InterPro

Analysis Name: InterPro Annotations of Lagenaria siceraria
Date Performed: 2017-09-18


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001623	DnaJ domain	PRINTS	PR00625	JDOMAIN	coord: 112..132 score: 7.7E-16coord: 132..151 score: 7.7E-16coord: 75..93 score: 7.7E-16coord: 93..108 score: 7.7
IPR001623	DnaJ domain	GENE3D	G3DSA:1.10.287.110		coord: 67..144 score: 2.1
IPR001623	DnaJ domain	PFAM	PF00226	DnaJ	coord: 74..137 score: 2.2
IPR001623	DnaJ domain	SMART	SM00271	dnaj_3	coord: 72..132 score: 4.2
IPR001623	DnaJ domain	PROFILE	PS50076	DNAJ_2	coord: 73..140 score: 19
IPR001623	DnaJ domain	unknown	SSF46565	Chaperone J-domain	coord: 73..146 score: 1.96
IPR018253	DnaJ domain, conserved site	PROSITE	PS00636	DNAJ_1	coord: 117..136 scor
None	No IPR available	PANTHER	PTHR24078	DNAJ HOMOLOG SUBFAMILY C MEMBER	coord: 74..138 score: 1.0
None	No IPR available	PANTHER	PTHR24078:SF222	SUBFAMILY NOT NAMED	coord: 74..138 score: 1.0

Synteny

The following gene(s) are orthologous to this gene:


Gene	Orthologue	Organism	Block
Lsi05G018600	Csa3G129700	Cucumber (Chinese Long) v2	culsiB209
Lsi05G018600	CSPI03G10650	Wild cucumber (PI 183967)	cpilsiB207
Lsi05G018600	Cucsa.256640	Cucumber (Gy14) v1	cgylsiB415
Lsi05G018600	CmaCh14G019070	Cucurbita maxima (Rimu)	cmalsiB245
Lsi05G018600	CmaCh06G012000	Cucurbita maxima (Rimu)	cmalsiB764
Lsi05G018600	CmoCh14G019620	Cucurbita moschata (Rifu)	cmolsiB233
Lsi05G018600	CmoCh06G012370	Cucurbita moschata (Rifu)	cmolsiB757
Lsi05G018600	CmoCh08G009710	Cucurbita moschata (Rifu)	cmolsiB823
Lsi05G018600	Cla021401	Watermelon (97103) v1	lsiwmB365
Lsi05G018600	MELO3C006295	Melon (DHL92) v3.5.1	lsimeB378
Lsi05G018600	ClCG05G003140	Watermelon (Charleston Gray)	lsiwcgB335
Lsi05G018600	MELO3C006295.2	Melon (DHL92) v3.6.1	lsimedB396
Lsi05G018600	Cla97C05G083770	Watermelon (97103) v2	lsiwmbB333
Lsi05G018600	Bhi01G000145	Wax gourd	lsiwgoB445
Lsi05G018600	Cp4.1LG03g18080	Cucurbita pepo (Zucchini)	cpelsiB506
Lsi05G018600	Cp4.1LG08g02660	Cucurbita pepo (Zucchini)	cpelsiB714
Lsi05G018600	CsGy3G010700	Cucumber (Gy14) v2	cgyblsiB196
Lsi05G018600	Carg06307	Silver-seed gourd	carlsiB878
Lsi05G018600	Carg11046	Silver-seed gourd	carlsiB983
Lsi05G018600	Carg10152	Silver-seed gourd	carlsiB206
Lsi05G018600	Carg24775	Silver-seed gourd	carlsiB156
Lsi05G018600	CsaV3_3G010760	Cucumber (Chinese Long) v3	cuclsiB228

The following gene(s) are paralogous to this gene:

None

The following block(s) are covering this gene:


Gene	Organism	Block
Lsi05G018600	Cucumber (Gy14) v2	cgyblsiB114
Lsi05G018600	Watermelon (97103) v2	lsiwmbB341
Lsi05G018600	Wax gourd	lsiwgoB421
Lsi05G018600	Bottle gourd (USVL1VR-Ls)	lsilsiB011
Lsi05G018600	Cucurbita maxima (Rimu)	cmalsiB356
Lsi05G018600	Cucurbita moschata (Rifu)	cmolsiB345
Lsi05G018600	Wild cucumber (PI 183967)	cpilsiB124
Lsi05G018600	Wild cucumber (PI 183967)	cpilsiB128
Lsi05G018600	Melon (DHL92) v3.5.1	lsimeB360
Lsi05G018600	Watermelon (Charleston Gray)	lsiwcgB343