CsGy3G010700 (gene) Cucumber (Gy14) v2

Overview
NameCsGy3G010700
Typegene
OrganismCucumis sativus (Cucumber (Gy14) v2)
DescriptionChaperone protein dnaJ 20, chloroplast
LocationChr3 : 8400980 .. 8402906 (-)
Genome Viewer
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRexonCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
AATGACTCAGCGTTGGACGTCTCGTATAAATAGCGGAATATGATAGAACGGAAGATAGACGGAGAAAAACAGAGAGAAATGCAGTGTTCCGATTTAACCTTTTCAGGGAGCGATTCACGCTTCTACATCCCTTCTAACCCTACCACTACCGCCCGCCGACCAATTTCCGGCTACCGATCTCGGGTTTGCTTTCCACACACTCCGCCGTTCAATTCGACGCGCTTGCCGTCTCTTTCAATTCGAGCCAAGGCCTCGTTCAACGAAGGATTCGTTTCCTCCGAGGTCGCCGAAGGCTCCTTCTACGATTTGCTCGGCATATCTAAGTCCGGATCCTTGGAGGAAATCAAGCGCGCGTACAAGCAGCTCGCTCGGAAGTACCATCCGGACGTTTCACCGCCGGGGTGTGTGGAGGAAAATACGAAGAGGTTCATTCGTGTTCAGGAGGCGTACGAAACGTTAGCAGATCCGAGGAGGAGAGCGCTGTATGATAGAGATATGATTGGAGGGCTTCAAGTCGCGTTCTCTGCTAGGAGACGATACGACGCCGATGAGGTATTATATTAGTTCACTCTACCGATTTCAACTTTCTTTTTTCGGTATTTTAAGGATAATCATGTTTAATTCTTAGCGGATTTTGGAACCTCCTTCGATTTTTTTGTGTTTCCCCTTGTTTTTTTAAAGTGATTTCGTGTTTAATTTCTCATATGGTGTCCATAGAGATTAGAATCAGTGAAGAATTTTAATCTCAATGATTTTGAATTTTTAAAGTGAGGAAAGGAAGCGTCGTTTTTCTTTTCCTCTTTCCTTTTGTTCTTCCGACTGTCTTATACGGACGCAGTACGATTGGTAGTGTATACTGTTCATGTTTAAACAAGGACCTATCGTTGATTATTCAGTGATTTGTTCTATGATCAAGAAACGGAATTGCACTAGCAAATCCTCCCAATATGGAATTCCGTTACTGTAAACCTCTAATTTTCCCTACTCTTCTGTTTACATTCAACATTTTCACCTAATTTCTTTCCTTCTCCATTAAATTCTTGTGTTCTACCGAACCTGTAAGGAATCCTTTATTTTTCTTCCTCCACTGAGTCTAACAAACAATCAATCCTATTGCAAAATTTCGATTTCCTATTTTCATATCATATACTCAACCTTCCAAATTTGATATTGGATTCAGGAAGTTGCACAGAAAAGTGGATGGAGAAACAGTTGGGAAGCTCAGATTTCGGAATTGAAGAGAAGAAGTATGGAGAAGGATTTGAGACCAAATATGTCATGGGGAGCTCGAATGCGCCGGCAAATGAACGAACAAAGTTGATGGAAATAGAATTATTCATATCAAGGAAAACCCATGTTTGACTCAGAATCAGTAAATAGAATACGGCTTATTCGGTAACATTAGCTTTTGTTGTTAGTTTTTTCTTTGTAATTTCCTTGAAACCTACTCTATGTGAAAAGCTTAACAATAAAAAGAAAAAGTGAGTCATTTTCAAATGACAAATAATTATATGCATTTCTACCCCAAATGCTCATTTTGATGCCCAAACCCGAGTATTACCCTTTGAAATATAGCAAAATATGAACAAAAGTATAGCAAAATTGTTGATTAACTTTTCTTATACTTTCTAAATATTTTTAGTAATTTTGTCATTTATGCAATAATTTTTCAATCTTTTTTCTTTTTAAATCTTATATACATAACAAATCTAAATCTTAAAAGTTAGAAAATCAAAACGAAATTTGTGATCATATGGTTCCTAACTTTGGTTTTATGTGAACCACGACATAGAGAACTTGAAGGAGACCATCAAATATCCTATCAATTTGGTCCTTTATTTATCATTTTTTCTTCTATATTTTTGGTCCCATACTTTGATGCAACTTAATTTTGTCCATTTGATAATGTTGATATCGATTTCCCCCT

mRNA sequence

AATGACTCAGCGTTGGACGTCTCGTATAAATAGCGGAATATGATAGAACGGAAGATAGACGGAGAAAAACAGAGAGAAATGCAGTGTTCCGATTTAACCTTTTCAGGGAGCGATTCACGCTTCTACATCCCTTCTAACCCTACCACTACCGCCCGCCGACCAATTTCCGGCTACCGATCTCGGGTTTGCTTTCCACACACTCCGCCGTTCAATTCGACGCGCTTGCCGTCTCTTTCAATTCGAGCCAAGGCCTCGTTCAACGAAGGATTCGTTTCCTCCGAGGTCGCCGAAGGCTCCTTCTACGATTTGCTCGGCATATCTAAGTCCGGATCCTTGGAGGAAATCAAGCGCGCGTACAAGCAGCTCGCTCGGAAGTACCATCCGGACGTTTCACCGCCGGGGTGTGTGGAGGAAAATACGAAGAGGTTCATTCGTGTTCAGGAGGCGTACGAAACGTTAGCAGATCCGAGGAGGAGAGCGCTGTATGATAGAGATATGATTGGAGGGCTTCAAGTCGCGTTCTCTGCTAGGAGACGATACGACGCCGATGAGGAAGTTGCACAGAAAAGTGGATGGAGAAACAGTTGGGAAGCTCAGATTTCGGAATTGAAGAGAAGAAGTATGGAGAAGGATTTGAGACCAAATATGTCATGGGGAGCTCGAATGCGCCGGCAAATGAACGAACAAAGTTGATGGAAATAGAATTATTCATATCAAGGAAAACCCATGTTTGACTCAGAATCAGTAAATAGAATACGGCTTATTCGGTAACATTAGCTTTTGTTGTTAGTTTTTTCTTTGTAATTTCCTTGAAACCTACTCTATGTGAAAAGCTTAACAATAAAAAGAAAAAGTGAGTCATTTTCAAATGACAAATAATTATATGCATTTCTACCCCAAATGCTCATTTTGATGCCCAAACCCGAGTATTACCCTTTGAAATATAGCAAAATATGAACAAAAGTATAGCAAAATTGTTGATTAACTTTTCTTATACTTTCTAAATATTTTTAGTAATTTTGTCATTTATGCAATAATTTTTCAATCTTTTTTCTTTTTAAATCTTATATACATAACAAATCTAAATCTTAAAAGTTAGAAAATCAAAACGAAATTTGTGATCATATGGTTCCTAACTTTGGTTTTATGTGAACCACGACATAGAGAACTTGAAGGAGACCATCAAATATCCTATCAATTTGGTCCTTTATTTATCATTTTTTCTTCTATATTTTTGGTCCCATACTTTGATGCAACTTAATTTTGTCCATTTGATAATGTTGATATCGATTTCCCCCT

Coding sequence (CDS)

ATGATAGAACGGAAGATAGACGGAGAAAAACAGAGAGAAATGCAGTGTTCCGATTTAACCTTTTCAGGGAGCGATTCACGCTTCTACATCCCTTCTAACCCTACCACTACCGCCCGCCGACCAATTTCCGGCTACCGATCTCGGGTTTGCTTTCCACACACTCCGCCGTTCAATTCGACGCGCTTGCCGTCTCTTTCAATTCGAGCCAAGGCCTCGTTCAACGAAGGATTCGTTTCCTCCGAGGTCGCCGAAGGCTCCTTCTACGATTTGCTCGGCATATCTAAGTCCGGATCCTTGGAGGAAATCAAGCGCGCGTACAAGCAGCTCGCTCGGAAGTACCATCCGGACGTTTCACCGCCGGGGTGTGTGGAGGAAAATACGAAGAGGTTCATTCGTGTTCAGGAGGCGTACGAAACGTTAGCAGATCCGAGGAGGAGAGCGCTGTATGATAGAGATATGATTGGAGGGCTTCAAGTCGCGTTCTCTGCTAGGAGACGATACGACGCCGATGAGGAAGTTGCACAGAAAAGTGGATGGAGAAACAGTTGGGAAGCTCAGATTTCGGAATTGAAGAGAAGAAGTATGGAGAAGGATTTGAGACCAAATATGTCATGGGGAGCTCGAATGCGCCGGCAAATGAACGAACAAAGTTGA

Protein sequence

MIERKIDGEKQREMQCSDLTFSGSDSRFYIPSNPTTTARRPISGYRSRVCFPHTPPFNSTRLPSLSIRAKASFNEGFVSSEVAEGSFYDLLGISKSGSLEEIKRAYKQLARKYHPDVSPPGCVEENTKRFIRVQEAYETLADPRRRALYDRDMIGGLQVAFSARRRYDADEEVAQKSGWRNSWEAQISELKRRSMEKDLRPNMSWGARMRRQMNEQS
Homology
[Close]
BLAST of CsGy3G010700 vs. NCBI nr
Match: XP_004133982.1 (PREDICTED: chaperone protein dnaJ 20, chloroplastic-like [Cucumis sativus] >KGN56713.1 Chaperone protein dnaJ 20, chloroplast [Cucumis sativus])

HSP 1 Score: 434.1 bits (1115), Expect = 2.8e-118
Identity = 217/217 (100.00%), Postives = 217/217 (100.00%), Query Frame = 0 

Query: 1   MIERKIDGEKQREMQCSDLTFSGSDSRFYIPSNPTTTARRPISGYRSRVCFPHTPPFNST 60
           MIERKIDGEKQREMQCSDLTFSGSDSRFYIPSNPTTTARRPISGYRSRVCFPHTPPFNST
Sbjct: 1   MIERKIDGEKQREMQCSDLTFSGSDSRFYIPSNPTTTARRPISGYRSRVCFPHTPPFNST 60

Query: 61  RLPSLSIRAKASFNEGFVSSEVAEGSFYDLLGISKSGSLEEIKRAYKQLARKYHPDVSPP 120
           RLPSLSIRAKASFNEGFVSSEVAEGSFYDLLGISKSGSLEEIKRAYKQLARKYHPDVSPP
Sbjct: 61  RLPSLSIRAKASFNEGFVSSEVAEGSFYDLLGISKSGSLEEIKRAYKQLARKYHPDVSPP 120

Query: 121 GCVEENTKRFIRVQEAYETLADPRRRALYDRDMIGGLQVAFSARRRYDADEEVAQKSGWR 180
           GCVEENTKRFIRVQEAYETLADPRRRALYDRDMIGGLQVAFSARRRYDADEEVAQKSGWR
Sbjct: 121 GCVEENTKRFIRVQEAYETLADPRRRALYDRDMIGGLQVAFSARRRYDADEEVAQKSGWR 180

Query: 181 NSWEAQISELKRRSMEKDLRPNMSWGARMRRQMNEQS 218
           NSWEAQISELKRRSMEKDLRPNMSWGARMRRQMNEQS
Sbjct: 181 NSWEAQISELKRRSMEKDLRPNMSWGARMRRQMNEQS 217

[Close]
BLAST of CsGy3G010700 vs. NCBI nr
Match: XP_008438327.1 (PREDICTED: chaperone protein dnaJ 20, chloroplastic-like [Cucumis melo])

HSP 1 Score: 396.0 bits (1016), Expect = 8.4e-107
Identity = 200/215 (93.02%), Postives = 205/215 (95.35%), Query Frame = 0 

Query: 2   IERKIDGEKQREMQCSDLTFSGSDSRFYIPSNPTTTARRPISGYRSRVCFPHTPPFNSTR 61
           IER+IDGEKQREMQCSD TFSGSDSRFYIPSNPT +ARRPISGYRSRV FPHTPPFNSTR
Sbjct: 15  IERRIDGEKQREMQCSDFTFSGSDSRFYIPSNPTISARRPISGYRSRVFFPHTPPFNSTR 74

Query: 62  LPSLSIRAKASFNEGFVSSEVAEGSFYDLLGISKSGSLEEIKRAYKQLARKYHPDVSPPG 121
           LPSLSIRAKASFNEGFVSSEVAEGSFYDLLGIS+SGSL EIKRAYKQLARKYHPDVSPPG
Sbjct: 75  LPSLSIRAKASFNEGFVSSEVAEGSFYDLLGISQSGSLAEIKRAYKQLARKYHPDVSPPG 134

Query: 122 CVEENTKRFIRVQEAYETLADPRRRALYDRDMIGGLQVAFSARRRYDADEEVAQKSGWRN 181
             EE TK FIRVQEAYETL+DPRRRALYDRDMIGGLQVAFSARRRYDA EEVA+KSGWRN
Sbjct: 135 GAEEYTKMFIRVQEAYETLSDPRRRALYDRDMIGGLQVAFSARRRYDAAEEVAEKSGWRN 194

Query: 182 SWEAQISELKRRSMEKDLRPNMSWGARMRRQMNEQ 217
           SWEAQISELKRRSMEKDLR NMSWGARMRRQMNEQ
Sbjct: 195 SWEAQISELKRRSMEKDLRQNMSWGARMRRQMNEQ 229

[Close]
BLAST of CsGy3G010700 vs. NCBI nr
Match: XP_022949677.1 (chaperone protein dnaJ 20, chloroplastic-like [Cucurbita moschata])

HSP 1 Score: 322.0 bits (824), Expect = 1.5e-84
Identity = 164/215 (76.28%), Postives = 182/215 (84.65%), Query Frame = 0 

Query: 2   IERKIDGEKQREMQCSDLTFSGSDSRFYIPSNPTTTARRPISGYRSRVCFPHTPPFNSTR 61
           +ER+    K REMQCSD T+S  D R  IPS+P+ T RR ISG RSRV FP+TPP NSTR
Sbjct: 26  VERR---RKNREMQCSDFTYSVGDYRILIPSSPSITGRRQISGQRSRVFFPNTPPCNSTR 85

Query: 62  LPSLSIRAKASFNEGFVSSEVAEGSFYDLLGISKSGSLEEIKRAYKQLARKYHPDVSPPG 121
           LPSLSIRAKASFN G  SSE A+GSFYDLLGIS+SGSL EIKRAYKQLARKYHPDVSPPG
Sbjct: 86  LPSLSIRAKASFNGGIASSEAADGSFYDLLGISESGSLAEIKRAYKQLARKYHPDVSPPG 145

Query: 122 CVEENTKRFIRVQEAYETLADPRRRALYDRDMIGGLQVAFSARRRYDADEEVAQKSGWRN 181
             EE TKRFIRVQEAYETL+DPRRRALYDRDM+GGLQVAFSARRRYD DE V  KS W++
Sbjct: 146 RAEEYTKRFIRVQEAYETLSDPRRRALYDRDMVGGLQVAFSARRRYDVDEGVPDKSAWKS 205

Query: 182 SWEAQISELKRRSMEKDLRPNMSWGARMRRQMNEQ 217
            W+AQISELK+RSM+KD + N+SWGARMR+QMNEQ
Sbjct: 206 CWKAQISELKKRSMDKDSKHNLSWGARMRQQMNEQ 237

[Close]
BLAST of CsGy3G010700 vs. NCBI nr
Match: XP_023526392.1 (chaperone protein dnaJ 20, chloroplastic-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 320.5 bits (820), Expect = 4.5e-84
Identity = 162/203 (79.80%), Postives = 178/203 (87.68%), Query Frame = 0 

Query: 14  MQCSDLTFSGSDSRFYIPSNPTTTARRPISGYRSRVCFPHTPPFNSTRLPSLSIRAKASF 73
           MQCSD TFSGSDSRFYIPS+P+ T RRPI+G+RS + FP+T P N  RLPSLSIRAKASF
Sbjct: 1   MQCSDFTFSGSDSRFYIPSSPSITGRRPITGHRSGIFFPNTLPCNLMRLPSLSIRAKASF 60

Query: 74  NEGFVSSEVAEGSFYDLLGISKSGSLEEIKRAYKQLARKYHPDVSPPGCVEENTKRFIRV 133
           NEG VSSEVA+GSFYDLLG+S+S SL EIKRAYKQLARKYHPDVSP G  EE TKRFIRV
Sbjct: 61  NEGTVSSEVADGSFYDLLGVSESVSLAEIKRAYKQLARKYHPDVSPIGRAEEYTKRFIRV 120

Query: 134 QEAYETLADPRRRALYDRDMIGGLQVAFSARRRYDADEEVAQKSGWRNSWEAQISELKRR 193
           QEAYETL+DPRRRALYDRDM+GGLQVAFSA R YDAD+E  +KSGWR+ W++QISELKRR
Sbjct: 121 QEAYETLSDPRRRALYDRDMVGGLQVAFSAWRLYDADQEAPEKSGWRSCWQSQISELKRR 180

Query: 194 SMEKDLRPNMSWGARMRRQMNEQ 217
           SM KD   NMSWGARMRRQMNEQ
Sbjct: 181 SMNKDSSQNMSWGARMRRQMNEQ 203

[Close]
BLAST of CsGy3G010700 vs. NCBI nr
Match: XP_022924259.1 (chaperone protein dnaJ 20, chloroplastic-like isoform X1 [Cucurbita moschata])

HSP 1 Score: 320.1 bits (819), Expect = 5.9e-84
Identity = 160/203 (78.82%), Postives = 176/203 (86.70%), Query Frame = 0 

Query: 14  MQCSDLTFSGSDSRFYIPSNPTTTARRPISGYRSRVCFPHTPPFNSTRLPSLSIRAKASF 73
           MQC D TFSGSDSRFYIPS+P+ T RRPI+G+RS + FP+T P N  RLPSLSIRAKASF
Sbjct: 1   MQCPDFTFSGSDSRFYIPSSPSITGRRPITGHRSGIFFPNTLPCNLMRLPSLSIRAKASF 60

Query: 74  NEGFVSSEVAEGSFYDLLGISKSGSLEEIKRAYKQLARKYHPDVSPPGCVEENTKRFIRV 133
           NEG VSSEVA+GSFYDLLG+S+S SL EIKRAYK LARKYHPDVSP GC EE TKRFIR 
Sbjct: 61  NEGIVSSEVADGSFYDLLGVSESVSLAEIKRAYKHLARKYHPDVSPIGCAEEYTKRFIRF 120

Query: 134 QEAYETLADPRRRALYDRDMIGGLQVAFSARRRYDADEEVAQKSGWRNSWEAQISELKRR 193
           QEAYETL+DPRRRALYDRDM+GGLQVAFSA R YDAD+E  +KSGWR+ W++QISELKRR
Sbjct: 121 QEAYETLSDPRRRALYDRDMVGGLQVAFSAWRLYDADQEAPEKSGWRSCWQSQISELKRR 180

Query: 194 SMEKDLRPNMSWGARMRRQMNEQ 217
           SM KD   NMSWGARMRRQMNEQ
Sbjct: 181 SMNKDSSQNMSWGARMRRQMNEQ 203

[Close]
BLAST of CsGy3G010700 vs. TAIR10
Match: AT4G13830.2 (DNAJ-like 20)

HSP 1 Score: 164.9 bits (416), Expect = 5.7e-41
Identity = 97/191 (50.79%), Postives = 128/191 (67.02%), Query Frame = 0 

Query: 33  NPTTTARRPISGYR-----SRVCFPHTPPFNSTRLPSLSIRAKASFNEGFVSSEVAEGSF 92
           +P    ++PIS  +     + + +P    F+STR     I+++ + ++    SE  + SF
Sbjct: 15  HPFFYKQQPISSLQPTSIPTTISYPTRTRFSSTR-----IQSRLTHDDPVKQSE--DLSF 74

Query: 93  YDLLGISKSGSLEEIKRAYKQLARKYHPDVSPPGCVEENTKRFIRVQEAYETLADPRRRA 152
           YDLLG+++S +L EIK+AYKQLARKYHPDVSPP  VEE T RFIRVQEAYETL+DPRRR 
Sbjct: 75  YDLLGVTESVTLPEIKQAYKQLARKYHPDVSPPDRVEEYTDRFIRVQEAYETLSDPRRRV 134

Query: 153 LYDRDMIGGLQVAFSARRRYDADEEVA-QKSGWRNSWEAQISELKRRSMEKDLRPNMSWG 212
           LYDRD+  G   +FS RR+   D+EV  +KS W+  W+ Q+S L+RRS +KD    MSW 
Sbjct: 135 LYDRDLSMGFSFSFSGRRQNRYDQEVVEEKSEWKAKWQTQLSGLRRRSNQKD-NNTMSWA 194

Query: 213 ARMRRQMNEQS 218
           ARMRRQ  E S
Sbjct: 195 ARMRRQQQESS 197

[Close]
BLAST of CsGy3G010700 vs. TAIR10
Match: AT2G22360.1 (DNAJ heat shock family protein)

HSP 1 Score: 69.3 bits (168), Expect = 3.3e-12
Identity = 47/138 (34.06%), Postives = 71/138 (51.45%), Query Frame = 0 

Query: 27  RFYIPSNPTTTARRPISGYRSRVCFPHTPPFNSTRLPSLSI----RAKASFNEGFVSSEV 86
           R Y PS   +T  +  S   + +    +  F+   LP LS+    R       G   +  
Sbjct: 23  RAYYPSRIESTRHQNSSSQVNCLGASKSSMFSHGSLPFLSMTGMSRNMHPPRRGSRFTVR 82

Query: 87  AEGSFYDLLGISKSGSLEEIKRAYKQLARKYHPDVSPPGCVEENTKRFIRVQEAYETLAD 146
           A+  +Y +LG+SK+ +  EIK AY++LAR YHPDV+     EE   +F  +  AYE L+D
Sbjct: 83  ADADYYSVLGVSKNATKAEIKSAYRKLARNYHPDVNKDPGAEE---KFKEISNAYEVLSD 142

Query: 147 PRRRALYDRDMIGGLQVA 161
             +++LYDR    GL+ A
Sbjct: 143 DEKKSLYDRYGEAGLKGA 157

[Close]
BLAST of CsGy3G010700 vs. TAIR10
Match: AT1G80030.1 (Molecular chaperone Hsp40/DnaJ family protein)

HSP 1 Score: 68.6 bits (166), Expect = 5.6e-12
Identity = 34/68 (50.00%), Postives = 49/68 (72.06%), Query Frame = 0 

Query: 85  GSFYDLLGISKSGSLEEIKRAYKQLARKYHPDVS-PPGCVEENTKRFIRVQEAYETLADP 144
           G +Y  LG+SKS + +EIK AY++LAR+YHPDV+  PG     T++F  +  AYE L+D 
Sbjct: 74  GDYYATLGVSKSANNKEIKAAYRRLARQYHPDVNKEPGA----TEKFKEISAAYEVLSDE 133

Query: 145 RRRALYDR 152
           ++RALYD+
Sbjct: 134 QKRALYDQ 137

[Close]
BLAST of CsGy3G010700 vs. TAIR10
Match: AT4G39960.1 (Molecular chaperone Hsp40/DnaJ family protein)

HSP 1 Score: 67.4 bits (163), Expect = 1.2e-11
Identity = 32/69 (46.38%), Postives = 46/69 (66.67%), Query Frame = 0 

Query: 83  AEGSFYDLLGISKSGSLEEIKRAYKQLARKYHPDVSPPGCVEENTKRFIRVQEAYETLAD 142
           A+  FY +LG+SK+ +  EIK AY++LAR YHPDV+     E+   +F  +  AYE L+D
Sbjct: 82  ADTDFYSVLGVSKNATKAEIKSAYRKLARSYHPDVNKDAGAED---KFKEISNAYEILSD 141

Query: 143 PRRRALYDR 152
             +R+LYDR
Sbjct: 142 DEKRSLYDR 147

[Close]
BLAST of CsGy3G010700 vs. TAIR10
Match: AT3G62600.1 (DNAJ heat shock family protein)

HSP 1 Score: 65.9 bits (159), Expect = 3.6e-11
Identity = 36/82 (43.90%), Postives = 53/82 (64.63%), Query Frame = 0 

Query: 82  VAEGSFYDLLGISKSGSLEEIKRAYKQLARKYHPDVSPPGCVEENTKRFIRVQEAYETLA 141
           +A  S+YD+L + K  S E+IKRAY++LA KYHPD +     EE T++F  +  AYE L+
Sbjct: 22  LAGKSYYDVLQVPKGASDEQIKRAYRKLALKYHPDKNQGN--EEATRKFAEINNAYEVLS 81

Query: 142 DPRRRALYDRDMIGGLQVAFSA 164
           D  +R +Y++    GL+  FSA
Sbjct: 82  DEEKREIYNKYGEEGLK-QFSA 100

[Close]
BLAST of CsGy3G010700 vs. Swiss-Prot
Match: sp|Q9SDN0|DNJ20_ARATH (Chaperone protein dnaJ 20, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=ATJ20 PE=1 SV=2)

HSP 1 Score: 164.9 bits (416), Expect = 1.0e-39
Identity = 97/191 (50.79%), Postives = 128/191 (67.02%), Query Frame = 0 

Query: 33  NPTTTARRPISGYR-----SRVCFPHTPPFNSTRLPSLSIRAKASFNEGFVSSEVAEGSF 92
           +P    ++PIS  +     + + +P    F+STR     I+++ + ++    SE  + SF
Sbjct: 15  HPFFYKQQPISSLQPTSIPTTISYPTRTRFSSTR-----IQSRLTHDDPVKQSE--DLSF 74

Query: 93  YDLLGISKSGSLEEIKRAYKQLARKYHPDVSPPGCVEENTKRFIRVQEAYETLADPRRRA 152
           YDLLG+++S +L EIK+AYKQLARKYHPDVSPP  VEE T RFIRVQEAYETL+DPRRR 
Sbjct: 75  YDLLGVTESVTLPEIKQAYKQLARKYHPDVSPPDRVEEYTDRFIRVQEAYETLSDPRRRV 134

Query: 153 LYDRDMIGGLQVAFSARRRYDADEEVA-QKSGWRNSWEAQISELKRRSMEKDLRPNMSWG 212
           LYDRD+  G   +FS RR+   D+EV  +KS W+  W+ Q+S L+RRS +KD    MSW 
Sbjct: 135 LYDRDLSMGFSFSFSGRRQNRYDQEVVEEKSEWKAKWQTQLSGLRRRSNQKD-NNTMSWA 194

Query: 213 ARMRRQMNEQS 218
           ARMRRQ  E S
Sbjct: 195 ARMRRQQQESS 197

[Close]
BLAST of CsGy3G010700 vs. Swiss-Prot
Match: sp|Q9Z9E9|DNAJ_CHLPN (Chaperone protein DnaJ OS=Chlamydia pneumoniae OX=83558 GN=dnaJ PE=3 SV=1)

HSP 1 Score: 75.5 bits (184), Expect = 8.2e-13
Identity = 37/65 (56.92%), Postives = 47/65 (72.31%), Query Frame = 0 

Query: 87  FYDLLGISKSGSLEEIKRAYKQLARKYHPDVSPPGCVEENTKRFIRVQEAYETLADPRRR 146
           +Y +LGISK+ S EEIK+AY++LA KYHPD +P     E  KRF  V EAYE L+DP++R
Sbjct: 3   YYSILGISKTASAEEIKKAYRKLAVKYHPDKNPGDAAAE--KRFKEVSEAYEVLSDPQKR 62

Query: 147 ALYDR 152
             YDR
Sbjct: 63  DSYDR 65

[Close]
BLAST of CsGy3G010700 vs. Swiss-Prot
Match: sp|A7GT07|DNAJ_BACCN (Chaperone protein DnaJ OS=Bacillus cytotoxicus (strain DSM 22905 / CIP 110041 / 391-98 / NVH 391-98) OX=315749 GN=dnaJ PE=3 SV=1)

HSP 1 Score: 75.1 bits (183), Expect = 1.1e-12
Identity = 37/71 (52.11%), Postives = 55/71 (77.46%), Query Frame = 0 

Query: 82  VAEGSFYDLLGISKSGSLEEIKRAYKQLARKYHPDVSPPGCVEEN-TKRFIRVQEAYETL 141
           +++  +Y++LG+SKS S +EIK+AY++LA+KYHPDVS     EEN  ++F  VQEAYE L
Sbjct: 1   MSKRDYYEVLGVSKSASKDEIKKAYRRLAKKYHPDVSK----EENAVEKFKEVQEAYEVL 60

Query: 142 ADPRRRALYDR 152
           +D ++RA YD+
Sbjct: 61  SDEQKRAQYDQ 67

[Close]
BLAST of CsGy3G010700 vs. Swiss-Prot
Match: sp|Q5L6F7|DNAJ_CHLAB (Chaperone protein DnaJ OS=Chlamydia abortus (strain DSM 27085 / S26/3) OX=218497 GN=dnaJ PE=3 SV=1)

HSP 1 Score: 75.1 bits (183), Expect = 1.1e-12
Identity = 36/65 (55.38%), Postives = 48/65 (73.85%), Query Frame = 0 

Query: 87  FYDLLGISKSGSLEEIKRAYKQLARKYHPDVSPPGCVEENTKRFIRVQEAYETLADPRRR 146
           +YD+LG+SK+ S EEIK++Y++LA KYHPD +P     E  KRF  V EAYE L+DP++R
Sbjct: 3   YYDVLGVSKTASPEEIKKSYRKLAVKYHPDKNPGDA--EAEKRFKEVSEAYEVLSDPQKR 62

Query: 147 ALYDR 152
             YDR
Sbjct: 63  ESYDR 65

[Close]
BLAST of CsGy3G010700 vs. Swiss-Prot
Match: sp|A5VJE8|DNAJ_LACRD (Chaperone protein DnaJ OS=Lactobacillus reuteri (strain DSM 20016) OX=557436 GN=dnaJ PE=3 SV=1)

HSP 1 Score: 75.1 bits (183), Expect = 1.1e-12
Identity = 36/70 (51.43%), Postives = 52/70 (74.29%), Query Frame = 0 

Query: 82  VAEGSFYDLLGISKSGSLEEIKRAYKQLARKYHPDVSPPGCVEENTKRFIRVQEAYETLA 141
           +AE  +YD+LG+SK  S ++IKRAY++LA KYHPDV+     EE   +F ++ EAYETL+
Sbjct: 1   MAEQDYYDILGVSKDASEKDIKRAYRRLAAKYHPDVNHEPGAEE---KFKKINEAYETLS 60

Query: 142 DPRRRALYDR 152
           D ++RA YD+
Sbjct: 61  DSQKRAQYDQ 67

[Close]
BLAST of CsGy3G010700 vs. TrEMBL
Match: tr|A0A0A0L4L9|A0A0A0L4L9_CUCSA (Chaperone protein dnaJ 20, chloroplast OS=Cucumis sativus OX=3659 GN=Csa_3G129700 PE=4 SV=1)

HSP 1 Score: 434.1 bits (1115), Expect = 1.8e-118
Identity = 217/217 (100.00%), Postives = 217/217 (100.00%), Query Frame = 0 

Query: 1   MIERKIDGEKQREMQCSDLTFSGSDSRFYIPSNPTTTARRPISGYRSRVCFPHTPPFNST 60
           MIERKIDGEKQREMQCSDLTFSGSDSRFYIPSNPTTTARRPISGYRSRVCFPHTPPFNST
Sbjct: 1   MIERKIDGEKQREMQCSDLTFSGSDSRFYIPSNPTTTARRPISGYRSRVCFPHTPPFNST 60

Query: 61  RLPSLSIRAKASFNEGFVSSEVAEGSFYDLLGISKSGSLEEIKRAYKQLARKYHPDVSPP 120
           RLPSLSIRAKASFNEGFVSSEVAEGSFYDLLGISKSGSLEEIKRAYKQLARKYHPDVSPP
Sbjct: 61  RLPSLSIRAKASFNEGFVSSEVAEGSFYDLLGISKSGSLEEIKRAYKQLARKYHPDVSPP 120

Query: 121 GCVEENTKRFIRVQEAYETLADPRRRALYDRDMIGGLQVAFSARRRYDADEEVAQKSGWR 180
           GCVEENTKRFIRVQEAYETLADPRRRALYDRDMIGGLQVAFSARRRYDADEEVAQKSGWR
Sbjct: 121 GCVEENTKRFIRVQEAYETLADPRRRALYDRDMIGGLQVAFSARRRYDADEEVAQKSGWR 180

Query: 181 NSWEAQISELKRRSMEKDLRPNMSWGARMRRQMNEQS 218
           NSWEAQISELKRRSMEKDLRPNMSWGARMRRQMNEQS
Sbjct: 181 NSWEAQISELKRRSMEKDLRPNMSWGARMRRQMNEQS 217

[Close]
BLAST of CsGy3G010700 vs. TrEMBL
Match: tr|A0A1S3AWS2|A0A1S3AWS2_CUCME (chaperone protein dnaJ 20, chloroplastic-like OS=Cucumis melo OX=3656 GN=LOC103483465 PE=4 SV=1)

HSP 1 Score: 396.0 bits (1016), Expect = 5.6e-107
Identity = 200/215 (93.02%), Postives = 205/215 (95.35%), Query Frame = 0 

Query: 2   IERKIDGEKQREMQCSDLTFSGSDSRFYIPSNPTTTARRPISGYRSRVCFPHTPPFNSTR 61
           IER+IDGEKQREMQCSD TFSGSDSRFYIPSNPT +ARRPISGYRSRV FPHTPPFNSTR
Sbjct: 15  IERRIDGEKQREMQCSDFTFSGSDSRFYIPSNPTISARRPISGYRSRVFFPHTPPFNSTR 74

Query: 62  LPSLSIRAKASFNEGFVSSEVAEGSFYDLLGISKSGSLEEIKRAYKQLARKYHPDVSPPG 121
           LPSLSIRAKASFNEGFVSSEVAEGSFYDLLGIS+SGSL EIKRAYKQLARKYHPDVSPPG
Sbjct: 75  LPSLSIRAKASFNEGFVSSEVAEGSFYDLLGISQSGSLAEIKRAYKQLARKYHPDVSPPG 134

Query: 122 CVEENTKRFIRVQEAYETLADPRRRALYDRDMIGGLQVAFSARRRYDADEEVAQKSGWRN 181
             EE TK FIRVQEAYETL+DPRRRALYDRDMIGGLQVAFSARRRYDA EEVA+KSGWRN
Sbjct: 135 GAEEYTKMFIRVQEAYETLSDPRRRALYDRDMIGGLQVAFSARRRYDAAEEVAEKSGWRN 194

Query: 182 SWEAQISELKRRSMEKDLRPNMSWGARMRRQMNEQ 217
           SWEAQISELKRRSMEKDLR NMSWGARMRRQMNEQ
Sbjct: 195 SWEAQISELKRRSMEKDLRQNMSWGARMRRQMNEQ 229

[Close]
BLAST of CsGy3G010700 vs. TrEMBL
Match: tr|A0A2P5C875|A0A2P5C875_9ROSA (Terminal organelle assembly protein OS=Trema orientalis OX=63057 GN=TorRG33x02_294110 PE=4 SV=1)

HSP 1 Score: 221.5 bits (563), Expect = 1.9e-54
Identity = 124/210 (59.05%), Postives = 153/210 (72.86%), Query Frame = 0 

Query: 14  MQCSDLTFSGSDSRFYIPSN--PTTTARRPISGYRSRVCFPHTP-PFNSTRLPSLSIRAK 73
           M+C  LT  GSDSRF I  N  P +T+R P             P P  STR+    ++AK
Sbjct: 1   MRCYGLTIPGSDSRFLISQNSTPVSTSRPPNPNLSPLFFTVRIPKPVYSTRIRVGPVKAK 60

Query: 74  A--SFNEGFVS-SEVAEGSFYDLLGISKSGSLEEIKRAYKQLARKYHPDVSPPGCVEENT 133
           A  + ++GFV+ +  AE SFY+LLGI +SGSL EIK+AYKQLARKYHPDVSPP  VEE T
Sbjct: 61  AASAVDDGFVAEAAAAELSFYELLGIPESGSLVEIKQAYKQLARKYHPDVSPPDRVEEYT 120

Query: 134 KRFIRVQEAYETLADPRRRALYDRDMIGGLQVAFSARRRYDADEEVAQKSGWRNSWEAQI 193
           +RFIRVQEAYETL+DPRRRA+YDRDM  GL +AFSARRR+  DE + +KS W+N W++Q+
Sbjct: 121 RRFIRVQEAYETLSDPRRRAMYDRDMAKGLHLAFSARRRHQNDEGLEEKSEWKNRWQSQV 180

Query: 194 SELKRRSMEKDLRPNMSWGARMRRQMNEQS 218
           SELKRRSM KD+  NMSWGARMRR+ ++ S
Sbjct: 181 SELKRRSMNKDVGGNMSWGARMRRRRDDLS 210

[Close]
BLAST of CsGy3G010700 vs. TrEMBL
Match: tr|A0A2P5A3X2|A0A2P5A3X2_PARAD (Terminal organelle assembly protein OS=Parasponia andersonii OX=3476 GN=PanWU01x14_371400 PE=4 SV=1)

HSP 1 Score: 221.1 bits (562), Expect = 2.4e-54
Identity = 123/210 (58.57%), Postives = 152/210 (72.38%), Query Frame = 0 

Query: 14  MQCSDLTFSGSDSRFYIP--SNPTTTARRPISGYRSRVCFPHTP-PFNSTRLPSLSIRAK 73
           M+C  LT SGSDSRF +P  S P +T R P             P P  S R+    ++AK
Sbjct: 1   MRCHGLTISGSDSRFLVPQYSTPISTPRPPNPNLSPLFFSVRIPKPVYSARIRVGPVKAK 60

Query: 74  A--SFNEGFVS-SEVAEGSFYDLLGISKSGSLEEIKRAYKQLARKYHPDVSPPGCVEENT 133
           A  + ++G V+ +  AE SFY+LLGI +SGSL +IK+AYKQLARKYHPDVSPP  VEE T
Sbjct: 61  AASAVDDGLVAEAAAAELSFYELLGIPESGSLVQIKQAYKQLARKYHPDVSPPDRVEEYT 120

Query: 134 KRFIRVQEAYETLADPRRRALYDRDMIGGLQVAFSARRRYDADEEVAQKSGWRNSWEAQI 193
           +RFIRVQEAYETL+DPRRRA+YDRDM  GL +AFSARRRY  DE + +KS W+  W++Q+
Sbjct: 121 RRFIRVQEAYETLSDPRRRAMYDRDMAKGLHLAFSARRRYQNDEGMEEKSEWKTRWQSQL 180

Query: 194 SELKRRSMEKDLRPNMSWGARMRRQMNEQS 218
           SELKRRSM KD+R NMSWGARMRR+ ++ S
Sbjct: 181 SELKRRSMNKDVRGNMSWGARMRRRRDDLS 210

[Close]
BLAST of CsGy3G010700 vs. TrEMBL
Match: tr|W9S291|W9S291_9ROSA (Chaperone protein dnaJ 20 OS=Morus notabilis OX=981085 GN=L484_011075 PE=4 SV=1)

HSP 1 Score: 217.2 bits (552), Expect = 3.5e-53
Identity = 121/203 (59.61%), Postives = 145/203 (71.43%), Query Frame = 0 

Query: 14  MQCSDLTFS-GSDSRFYIPSNPTTTARRPISGYRSRVCFPHTPPFNSTRLPSLSIRAKAS 73
           M+C+ L  S GSDSRF+ P+N +T          S V FP  P F STR+ +     +AS
Sbjct: 1   MRCNGLAISGGSDSRFFFPTNSSTIYTASARNPSSTVLFPGRPVF-STRIRA---TPRAS 60

Query: 74  FNEGFVSSEVAEGSFYDLLGISKSGSLEEIKRAYKQLARKYHPDVSPPGCVEENTKRFIR 133
                V   VAE SFY+LLGI +SGSL EIK+AYKQLARKYHPDVSPPG VEE T+ FIR
Sbjct: 61  AAAAVVGDRVAELSFYELLGIPESGSLGEIKQAYKQLARKYHPDVSPPGRVEEYTRTFIR 120

Query: 134 VQEAYETLADPRRRALYDRDMIGGLQVAFSARRRYDADEEVAQKSGWRNSWEAQISELKR 193
           VQEAYETL+DPR RA+YDRDM  GL +AFSARRRY +DE + +KS W    ++Q+SELKR
Sbjct: 121 VQEAYETLSDPRLRAIYDRDMAKGLHLAFSARRRYQSDEGMEEKSEWXXXXQSQLSELKR 180

Query: 194 RSMEKDLRPNMSWGARMRRQMNE 216
           RS+ KD R NMSWGARMRR+M +
Sbjct: 181 RSVNKDSRGNMSWGARMRRRMED 199

The following BLAST results are available for this feature:
BLAST of CsGy3G010700 vs. NCBI nr
Match NameE-valueIdentityDescription
XP_004133982.12.8e-118100.00PREDICTED: chaperone protein dnaJ 20, chloroplastic-like [Cucumis sativus] >KGN5... [more]
XP_008438327.18.4e-10793.02PREDICTED: chaperone protein dnaJ 20, chloroplastic-like [Cucumis melo][more]
XP_022949677.11.5e-8476.28chaperone protein dnaJ 20, chloroplastic-like [Cucurbita moschata][more]
XP_023526392.14.5e-8479.80chaperone protein dnaJ 20, chloroplastic-like [Cucurbita pepo subsp. pepo][more]
XP_022924259.15.9e-8478.82chaperone protein dnaJ 20, chloroplastic-like isoform X1 [Cucurbita moschata][more]
BLAST of CsGy3G010700 vs. TAIR10
Match NameE-valueIdentityDescription
AT4G13830.25.7e-4150.79DNAJ-like 20[more]
AT2G22360.13.3e-1234.06DNAJ heat shock family protein[more]
AT1G80030.15.6e-1250.00Molecular chaperone Hsp40/DnaJ family protein[more]
AT4G39960.11.2e-1146.38Molecular chaperone Hsp40/DnaJ family protein[more]
AT3G62600.13.6e-1143.90DNAJ heat shock family protein[more]
BLAST of CsGy3G010700 vs. Swiss-Prot
Match NameE-valueIdentityDescription
sp|Q9SDN0|DNJ20_ARATH1.0e-3950.79Chaperone protein dnaJ 20, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=ATJ2... [more]
sp|Q9Z9E9|DNAJ_CHLPN8.2e-1356.92Chaperone protein DnaJ OS=Chlamydia pneumoniae OX=83558 GN=dnaJ PE=3 SV=1[more]
sp|A7GT07|DNAJ_BACCN1.1e-1252.11Chaperone protein DnaJ OS=Bacillus cytotoxicus (strain DSM 22905 / CIP 110041 / ... [more]
sp|Q5L6F7|DNAJ_CHLAB1.1e-1255.38Chaperone protein DnaJ OS=Chlamydia abortus (strain DSM 27085 / S26/3) OX=218497... [more]
sp|A5VJE8|DNAJ_LACRD1.1e-1251.43Chaperone protein DnaJ OS=Lactobacillus reuteri (strain DSM 20016) OX=557436 GN=... [more]
BLAST of CsGy3G010700 vs. TrEMBL
Match NameE-valueIdentityDescription
tr|A0A0A0L4L9|A0A0A0L4L9_CUCSA1.8e-118100.00Chaperone protein dnaJ 20, chloroplast OS=Cucumis sativus OX=3659 GN=Csa_3G12970... [more]
tr|A0A1S3AWS2|A0A1S3AWS2_CUCME5.6e-10793.02chaperone protein dnaJ 20, chloroplastic-like OS=Cucumis melo OX=3656 GN=LOC1034... [more]
tr|A0A2P5C875|A0A2P5C875_9ROSA1.9e-5459.05Terminal organelle assembly protein OS=Trema orientalis OX=63057 GN=TorRG33x02_2... [more]
tr|A0A2P5A3X2|A0A2P5A3X2_PARAD2.4e-5458.57Terminal organelle assembly protein OS=Parasponia andersonii OX=3476 GN=PanWU01x... [more]
tr|W9S291|W9S291_9ROSA3.5e-5359.61Chaperone protein dnaJ 20 OS=Morus notabilis OX=981085 GN=L484_011075 PE=4 SV=1[more]
Annotated Terms
The following terms have been associated with this gene:
Vocabulary: INTERPRO
TermDefinition
IPR018253DnaJ_domain_CS
IPR036869J_dom_sf
IPR001623DnaJ_domain
GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0008150 biological_process
cellular_component GO:0005575 cellular_component
molecular_function GO:0003674 molecular_function
Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CsGy3G010700.1CsGy3G010700.1mRNA


InterPro
Analysis Name: InterPro Annotations of cucumber Gy14 genome (v2)
Date Performed: 2018-09-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001623DnaJ domainPRINTSPR00625JDOMAINcoord: 106..121
score: 48.68
coord: 125..145
score: 35.96
coord: 145..164
score: 23.64
coord: 88..106
score: 48.04
IPR001623DnaJ domainSMARTSM00271dnaj_3coord: 85..145
e-value: 2.3E-20
score: 83.7
IPR001623DnaJ domainPFAMPF00226DnaJcoord: 87..150
e-value: 1.8E-20
score: 72.7
IPR001623DnaJ domainPROSITEPS50076DNAJ_2coord: 86..153
score: 20.357
IPR001623DnaJ domainCDDcd06257DnaJcoord: 87..142
e-value: 5.90464E-19
score: 76.8153
IPR036869Chaperone J-domain superfamilyGENE3DG3DSA:1.10.287.110coord: 78..185
e-value: 1.4E-22
score: 81.7
IPR036869Chaperone J-domain superfamilySUPERFAMILYSSF46565Chaperone J-domaincoord: 86..160
NoneNo IPR availablePANTHERPTHR45090FAMILY NOT NAMEDcoord: 52..217
IPR018253DnaJ domain, conserved sitePROSITEPS00636DNAJ_1coord: 130..149

Synteny
The following gene(s) are orthologous to this gene:
GeneOrthologueOrganismBlock
CsGy3G010700Cla021401Watermelon (97103) v1cgybwmB217
CsGy3G010700Csa3G129700Cucumber (Chinese Long) v2cgybcuB109
CsGy3G010700MELO3C006295Melon (DHL92) v3.5.1cgybmeB200
CsGy3G010700ClCG05G003140Watermelon (Charleston Gray)cgybwcgB203
CsGy3G010700CSPI03G10650Wild cucumber (PI 183967)cgybcpiB110
CsGy3G010700CmaCh14G019070Cucurbita maxima (Rimu)cgybcmaB318
CsGy3G010700CmaCh17G000850Cucurbita maxima (Rimu)cgybcmaB345
CsGy3G010700CmaCh06G012000Cucurbita maxima (Rimu)cgybcmaB427
CsGy3G010700CmoCh14G019620Cucurbita moschata (Rifu)cgybcmoB290
CsGy3G010700CmoCh06G012370Cucurbita moschata (Rifu)cgybcmoB401
CsGy3G010700CmoCh17G000910Cucurbita moschata (Rifu)cgybcmoB317
CsGy3G010700Lsi05G018600Bottle gourd (USVL1VR-Ls)cgyblsiB196
CsGy3G010700Cp4.1LG08g02660Cucurbita pepo (Zucchini)cgybcpeB454
CsGy3G010700Cp4.1LG03g18080Cucurbita pepo (Zucchini)cgybcpeB406
CsGy3G010700Cp4.1LG12g00650Cucurbita pepo (Zucchini)cgybcpeB304
CsGy3G010700MELO3C006295.2Melon (DHL92) v3.6.1cgybmedB201
CsGy3G010700CsaV3_3G010760Cucumber (Chinese Long) v3cgybcucB117
CsGy3G010700Cla97C05G083770Watermelon (97103) v2cgybwmbB196
CsGy3G010700Bhi01G000145Wax gourdcgybwgoB258
CsGy3G010700Carg11046Silver-seed gourdcarcgybB1059
CsGy3G010700Carg06307Silver-seed gourdcarcgybB0948
CsGy3G010700Carg10152Silver-seed gourdcarcgybB0245
CsGy3G010700Carg24775Silver-seed gourdcarcgybB0187
The following gene(s) are paralogous to this gene:

None

The following block(s) are covering this gene:
GeneOrganismBlock
CsGy3G010700Bottle gourd (USVL1VR-Ls)cgyblsiB136
CsGy3G010700Melon (DHL92) v3.5.1cgybmeB195
CsGy3G010700Watermelon (Charleston Gray)cgybwcgB230
CsGy3G010700Wild cucumber (PI 183967)cgybcpiB109
CsGy3G010700Watermelon (97103) v2cgybwmbB222
CsGy3G010700Wax gourdcgybwgoB242
CsGy3G010700Cucumber (Gy14) v2cgybcgybB054