Lsi05G015850 (gene) Bottle gourd (USVL1VR-Ls) | Cucurbit Genomics Database (CuGenDB)

Overview


Name	Lsi05G015850
Type	gene
Organism	Lagenaria siceraria (Bottle gourd (USVL1VR-Ls))
Description	DNA-directed RNA polymerase subunit
Location	chr05 : 23614441 .. 23617266 (+)

Genome Viewer

Gene Expression

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

AGATAATAGTGTAATATGCAGTTACAATTAAGACCTTCTATGTATAAAACCCCTAAGACTACCCCTTAATTTACTGGGCTACCAAAATTTGATTTGGGTTCTGGAATTCGAAGCCCATTTACAATTTCTTCAGTTTCTCCTGCCATTGGAAGAAAACTCCTCTTTCGCCTTATCGACGTTTCTAGAACCGCAGGGCTGATTTTTGATCGTCTTCTTTACACTCTACTCAAACTCTTCAAAATCTATCATTTTCTTTGATATACTCTTAGATTATCTCGCAGATTCCTTCTGATTCTCAGCTGCTAAAACCCTAGGGAGAAATTTATCTTTGTTTAGCTCAGGGTTTGTTGTTTTTCTACCTTCTGCAAGATGAGTACCATGAAGTTCTGCCGGGAATGGTATGCCTTATCATAACCACACACTCGCGTACTTGCGCTCAATGATCGCACATGGCTTTCATTCTTCTGTGTTGATTAGTTTAAGTTTTTGGAAAATTTTGTAATCATTGAAATTTTTATGTTGCAGTAACAACATTCTCTATCCGAAAGAGGATAGAGATGAGAAGATTCTTCTCTATGCGTGTCGTAACTGTGATCACCAGGTACCTTCTCTTTCTTTTGATTTCATCTTTCTATTTTAATGTTGTGAACATTTTAGGTTTTTTAGTTAGCCTTTCATTTCTAATGTATATTAGTTGCGACTTACGATCACGGTGCTCTCTTTTCATATTTTGTCTGGTCTCTGGTGAAATCCTTTGTTATGGTTTTCTTCCTGTAGTTAAAATCATATATCAATTTGTTTTTTTAATGTAAATTGTCTTGCTTTGTAGTAGTTTTGACCTAGGATATGGTTATTTGGACCTGATTCATGATTATGCCCTCAAATGCTCGAGGTTCCCCCTTTAGTTCGGCGCACTGTGTTAAAGTTTTACTTATGGCGTCCTATTACCTCTGTTTTTTATCTTACCTTTCTCTGAACTTGGATGGGAACCTTTGTTCTTCGCTGCTTTTGTAGTCCATTTTCATATCAATAAATTTCAGTAAGGGAAAATTTATGAACATGATGATTTTAACTAGTTATCATCTGTTAATGAAATGCTAACGGGCCCTTACCGTCCTTAGTTGAAGATATGCATGTATAACTGTGAATATAATTGGAGAAAAATGGTAGAAACAGTCTCTTGTTTCATTTTATCCAGGAGGGGGAATGTAACCTTTTTTGGGATGGTTAATTCAGCCTTTATAAAATTTTTAGGATAATATATATTACTTATTTAATTCTGAAGGTTGCAAGTTTAATCTAATTTTTATTTGATGTTAGAGTGTTATCTCATTCCCTGAGATCACGAAATTGTCAAAGTTAATCTTCATGAGTTGCCTTTGATTCCCCAGTTCTTTTTTATTTATTTGTGTTCTTAAGTTCCTTGGCATGTTACTTATGGTGGGAGACTGTAAGGTTTTGAGCCTTGTTCTTTACCAATTGAATTGCATATCTTTACTCGGTATATGTTTTCTTGTTGCGCCATTGTTGAAAACTTCCTTTCTGCTTTTGTGTAACGAAAGGAATTTTATTTTTACCTACTGGAAGATAGACCTGCTTTTGTATTCTGAGATTGATGTTTTCACCATTATTGTAGCCTTATTTATTTGTTCATATTGCAACAAAATCATCCCATTGATGGAGTGGAAATATACGTACCCATATTTTTCCGTCGCTGTCCTCCCTCTTTCACTTACAGATCCTCTTCTCTGTGTATGGACTTGTTTTATTTCTAGATATTGATGGTTTCCTCCCCTCTTATTCTGCCGGTTAATATTCTTTATTTTGTTATTTTATAAAAATTGTATTCACAGGAAGTTGCTGAAAATAACTGCGTCTATAGGAATGAGATTCATCACGCTGTAGGGGAACGCACTCAAGTTCTGCAAGATGTTGCTGCAGATCCAACTCTCCCTCGCACTAAAGCTGTCCGATGTGCTCAATGCAACCATGGAGAAGCTGTTTTTTTCCAGGTCCACTTCCTTTTTTCCCCCTTTTGGAAAGCCTTCGTTTGTTTGCAAGGATTGGGCTTTCTTTTCCTCTATTCTCTCATTTATCACCGTTAAAGGGTTTTTTAACACATGCTTTATTCTTTTCAACTCCCAAAACTTTAAGTTGGTTCTGTTTTTCATTCTACATCATCATCTTCTTAATACGGTTGTGAATACTGATAAGGTTTTCTTGAATGAGAAATCAGAATTGTTAGTTTGATTGTGTAATTGAGTTGGATATTTTGTCAAGCTGCTTGGAGGTTATAAATTAAAATTACATCATTTCATCATTGTTACTTGTATTTAAGCCTAGATATCATTGCGTTCTGTCTGCAGGCCACTGCCAGAGGGGAGGAAGGTATGACATTGTTCTTCGTCTGCTGCAATCCAAATTGTGGCCACAGATGGAGAGATTGATATCAAATACATGCCGAATTTTTCCTTTGTGCAATCGAAGTTTTATTGGCACACCAAATGTTTTAACCATCTTATGCTTGAAGACAATATGTTTTTAACATGGGATTTACAATTGTACTGATTGTCTGTGGGTTGATGAGAACCATTAACTTCGCAACTTCTCAACTTCTCAATTTAACTTTGCAGAGATGTGCTGGAAACAATGCTGTTTGGGAGTGTTTTAAAATTATTTGTCATTTTTTAAATTGTTTGTGTTTTTAATCATTCAAAATTAATTTTGACTAGATGAGAATTGCATTAAAAAAAAAAGTTAAATTAAAATCGATTTTGAGTTATTAAAGATGTATTTTGAAGTAGTTTCGAATATGATAAAAAGTAA

mRNA sequence

AGATAATAGTGTAATATGCAGTTACAATTAAGACCTTCTATGTATAAAACCCCTAAGACTACCCCTTAATTTACTGGGCTACCAAAATTTGATTTGGGTTCTGGAATTCGAAGCCCATTTACAATTTCTTCAGTTTCTCCTGCCATTGGAAGAAAACTCCTCTTTCGCCTTATCGACGTTTCTAGAACCGCAGGGCTGATTTTTGATCGTCTTCTTTACACTCTACTCAAACTCTTCAAAATCTATCATTTTCTTTGATATACTCTTAGATTATCTCGCAGATTCCTTCTGATTCTCAGCTGCTAAAACCCTAGGGAGAAATTTATCTTTGTTTAGCTCAGGGTTTGTTGTTTTTCTACCTTCTGCAAGATGAGTACCATGAAGTTCTGCCGGGAATGTAACAACATTCTCTATCCGAAAGAGGATAGAGATGAGAAGATTCTTCTCTATGCGTGTCGTAACTGTGATCACCAGGAAGTTGCTGAAAATAACTGCGTCTATAGGAATGAGATTCATCACGCTGTAGGGGAACGCACTCAAGTTCTGCAAGATGTTGCTGCAGATCCAACTCTCCCTCGCACTAAAGCTGTCCGATGTGCTCAATGCAACCATGGAGAAGCTGTTTTTTTCCAGGCCACTGCCAGAGGGGAGGAAGGTATGACATTGTTCTTCGTCTGCTGCAATCCAAATTGTGGCCACAGATGGAGAGATTGATATCAAATACATGCCGAATTTTTCCTTTGTGCAATCGAAGTTTTATTGGCACACCAAATGTTTTAACCATCTTATGCTTGAAGACAATATGTTTTTAACATGGGATTTACAATTGTACTGATTGTCTGTGGGTTGATGAGAACCATTAACTTCGCAACTTCTCAACTTCTCAATTTAACTTTGCAGAGATGTGCTGGAAACAATGCTGTTTGGGAGTGTTTTAAAATTATTTGTCATTTTTTAAATTGTTTGTGTTTTTAATCATTCAAAATTAATTTTGACTAGATGAGAATTGCATTAAAAAAAAAAGTTAAATTAAAATCGATTTTGAGTTATTAAAGATGTATTTTGAAGTAGTTTCGAATATGATAAAAAGTAA

Coding sequence (CDS)

ATGAGTACCATGAAGTTCTGCCGGGAATGTAACAACATTCTCTATCCGAAAGAGGATAGAGATGAGAAGATTCTTCTCTATGCGTGTCGTAACTGTGATCACCAGGAAGTTGCTGAAAATAACTGCGTCTATAGGAATGAGATTCATCACGCTGTAGGGGAACGCACTCAAGTTCTGCAAGATGTTGCTGCAGATCCAACTCTCCCTCGCACTAAAGCTGTCCGATGTGCTCAATGCAACCATGGAGAAGCTGTTTTTTTCCAGGCCACTGCCAGAGGGGAGGAAGGTATGACATTGTTCTTCGTCTGCTGCAATCCAAATTGTGGCCACAGATGGAGAGATTGA

Protein sequence

MSTMKFCRECNNILYPKEDRDEKILLYACRNCDHQEVAENNCVYRNEIHHAVGERTQVLQDVAADPTLPRTKAVRCAQCNHGEAVFFQATARGEEGMTLFFVCCNPNCGHRWRD

Homology

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BLAST of Lsi05G015850 vs. Swiss-Prot
Match: RPB9A_ARATH (DNA-directed RNA polymerases II, IV and V subunit 9A OS=Arabidopsis thaliana GN=NRPB9A PE=1 SV=1)

HSP 1 Score: 229.2 bits (583), Expect = 2.3e-59
Identity = 98/114 (85.96%), Postives = 110/114 (96.49%), Query Frame = 1


Query: 1   MSTMKFCRECNNILYPKEDRDEKILLYACRNCDHQEVAENNCVYRNEIHHAVGERTQVLQ 60
           MSTMKFCRECNNILYPKED+++KILLYACRNCDHQEVA+N+CVYRNE+HH+V ERTQ+L 
Sbjct: 1   MSTMKFCRECNNILYPKEDKEQKILLYACRNCDHQEVADNSCVYRNEVHHSVSERTQILT 60

Query: 61  DVAADPTLPRTKAVRCAQCNHGEAVFFQATARGEEGMTLFFVCCNPNCGHRWRD 115
           DVA+DPTLPRTKAVRC++C H EAVFFQATARGEEGMTLFFVCCNPNCGHRWR+
Sbjct: 61  DVASDPTLPRTKAVRCSKCQHREAVFFQATARGEEGMTLFFVCCNPNCGHRWRE 114

[Close]

BLAST of Lsi05G015850 vs. Swiss-Prot
Match: RPB9B_ARATH (DNA-directed RNA polymerases II, IV and V subunit 9B OS=Arabidopsis thaliana GN=NRPB9B PE=1 SV=1)

HSP 1 Score: 228.4 bits (581), Expect = 3.9e-59
Identity = 97/114 (85.09%), Postives = 108/114 (94.74%), Query Frame = 1


Query: 1   MSTMKFCRECNNILYPKEDRDEKILLYACRNCDHQEVAENNCVYRNEIHHAVGERTQVLQ 60
           MSTMKFCRECNNILYPKED+++ ILLYACRNCDHQE A+NNCVYRNE+HH+V E+TQ+L 
Sbjct: 1   MSTMKFCRECNNILYPKEDKEQSILLYACRNCDHQEAADNNCVYRNEVHHSVSEQTQILS 60

Query: 61  DVAADPTLPRTKAVRCAQCNHGEAVFFQATARGEEGMTLFFVCCNPNCGHRWRD 115
           DVA+DPTLPRTKAVRCA+C HGEAVFFQATARGEEGMTLFFVCCNPNC HRWR+
Sbjct: 61  DVASDPTLPRTKAVRCAKCQHGEAVFFQATARGEEGMTLFFVCCNPNCSHRWRE 114

[Close]

BLAST of Lsi05G015850 vs. Swiss-Prot
Match: RPB9_BOVIN (DNA-directed RNA polymerase II subunit RPB9 OS=Bos taurus GN=POLR2I PE=1 SV=1)

HSP 1 Score: 156.8 bits (395), Expect = 1.5e-37
Identity = 65/112 (58.04%), Postives = 90/112 (80.36%), Query Frame = 1


Query: 4   MKFCRECNNILYPKEDRDEKILLYACRNCDHQEVAENNCVYRNEIHHAVGERTQVLQDVA 63
           ++FC+ECNN+LYPKED++ +ILLYACRNCD+Q+ A+N+C+Y N+I H V E TQ++ DV+
Sbjct: 14  IRFCQECNNMLYPKEDKENRILLYACRNCDYQQEADNSCIYVNKITHEVDELTQIIADVS 73

Query: 64  ADPTLPRTKAVRCAQCNHGEAVFFQA-TARGEEGMTLFFVCCNPNCGHRWRD 115
            DPTLPRT+   C +C H EAVFFQ+ +AR E+ M L++VC  P+CGHRW +
Sbjct: 74  QDPTLPRTEDHPCQKCGHKEAVFFQSHSARAEDAMRLYYVCTAPHCGHRWTE 125

[Close]

BLAST of Lsi05G015850 vs. Swiss-Prot
Match: RPB9_HUMAN (DNA-directed RNA polymerase II subunit RPB9 OS=Homo sapiens GN=POLR2I PE=1 SV=1)

HSP 1 Score: 156.8 bits (395), Expect = 1.5e-37
Identity = 65/112 (58.04%), Postives = 90/112 (80.36%), Query Frame = 1


Query: 4   MKFCRECNNILYPKEDRDEKILLYACRNCDHQEVAENNCVYRNEIHHAVGERTQVLQDVA 63
           ++FC+ECNN+LYPKED++ +ILLYACRNCD+Q+ A+N+C+Y N+I H V E TQ++ DV+
Sbjct: 14  IRFCQECNNMLYPKEDKENRILLYACRNCDYQQEADNSCIYVNKITHEVDELTQIIADVS 73

Query: 64  ADPTLPRTKAVRCAQCNHGEAVFFQA-TARGEEGMTLFFVCCNPNCGHRWRD 115
            DPTLPRT+   C +C H EAVFFQ+ +AR E+ M L++VC  P+CGHRW +
Sbjct: 74  QDPTLPRTEDHPCQKCGHKEAVFFQSHSARAEDAMRLYYVCTAPHCGHRWTE 125

[Close]

BLAST of Lsi05G015850 vs. Swiss-Prot
Match: RPB9_MOUSE (DNA-directed RNA polymerase II subunit RPB9 OS=Mus musculus GN=Polr2i PE=1 SV=1)

HSP 1 Score: 156.8 bits (395), Expect = 1.5e-37
Identity = 65/112 (58.04%), Postives = 90/112 (80.36%), Query Frame = 1


Query: 4   MKFCRECNNILYPKEDRDEKILLYACRNCDHQEVAENNCVYRNEIHHAVGERTQVLQDVA 63
           ++FC+ECNN+LYPKED++ +ILLYACRNCD+Q+ A+N+C+Y N+I H V E TQ++ DV+
Sbjct: 14  IRFCQECNNMLYPKEDKENRILLYACRNCDYQQEADNSCIYVNKITHEVDELTQIIADVS 73

Query: 64  ADPTLPRTKAVRCAQCNHGEAVFFQA-TARGEEGMTLFFVCCNPNCGHRWRD 115
            DPTLPRT+   C +C H EAVFFQ+ +AR E+ M L++VC  P+CGHRW +
Sbjct: 74  QDPTLPRTEDHPCQKCGHKEAVFFQSHSARAEDAMRLYYVCTAPHCGHRWTE 125

[Close]

BLAST of Lsi05G015850 vs. TrEMBL
Match: A0A0A0L816_CUCSA (DNA-directed RNA polymerase subunit OS=Cucumis sativus GN=Csa_3G166240 PE=3 SV=1)

HSP 1 Score: 252.3 bits (643), Expect = 2.8e-64
Identity = 113/114 (99.12%), Postives = 114/114 (100.00%), Query Frame = 1


Query: 1   MSTMKFCRECNNILYPKEDRDEKILLYACRNCDHQEVAENNCVYRNEIHHAVGERTQVLQ 60
           MSTMKFCRECNNILYPKEDRDEKILLYACRNCDHQEVA+NNCVYRNEIHHAVGERTQVLQ
Sbjct: 1   MSTMKFCRECNNILYPKEDRDEKILLYACRNCDHQEVADNNCVYRNEIHHAVGERTQVLQ 60

Query: 61  DVAADPTLPRTKAVRCAQCNHGEAVFFQATARGEEGMTLFFVCCNPNCGHRWRD 115
           DVAADPTLPRTKAVRCAQCNHGEAVFFQATARGEEGMTLFFVCCNPNCGHRWRD
Sbjct: 61  DVAADPTLPRTKAVRCAQCNHGEAVFFQATARGEEGMTLFFVCCNPNCGHRWRD 114

[Close]

BLAST of Lsi05G015850 vs. TrEMBL
Match: A0A103XBI1_CYNCS (DNA-directed RNA polymerase subunit OS=Cynara cardunculus var. scolymus GN=Ccrd_025042 PE=3 SV=1)

HSP 1 Score: 247.7 bits (631), Expect = 7.0e-63
Identity = 110/114 (96.49%), Postives = 113/114 (99.12%), Query Frame = 1


Query: 1   MSTMKFCRECNNILYPKEDRDEKILLYACRNCDHQEVAENNCVYRNEIHHAVGERTQVLQ 60
           MSTMKFCRECNNILYPKED+D+KILLYACRNCDHQEVA+NNCVYRNEIHHAVGERTQVLQ
Sbjct: 1   MSTMKFCRECNNILYPKEDKDQKILLYACRNCDHQEVADNNCVYRNEIHHAVGERTQVLQ 60

Query: 61  DVAADPTLPRTKAVRCAQCNHGEAVFFQATARGEEGMTLFFVCCNPNCGHRWRD 115
           DVAADPTLPRTKAVRCAQC HGEAVFFQATARGEEGMTLFFVCCNPNCGHRWRD
Sbjct: 61  DVAADPTLPRTKAVRCAQCGHGEAVFFQATARGEEGMTLFFVCCNPNCGHRWRD 114

[Close]

BLAST of Lsi05G015850 vs. TrEMBL
Match: A5AGZ7_VITVI (DNA-directed RNA polymerase subunit OS=Vitis vinifera GN=VIT_05s0077g01170 PE=3 SV=1)

HSP 1 Score: 246.1 bits (627), Expect = 2.0e-62
Identity = 109/114 (95.61%), Postives = 114/114 (100.00%), Query Frame = 1


Query: 1   MSTMKFCRECNNILYPKEDRDEKILLYACRNCDHQEVAENNCVYRNEIHHAVGERTQVLQ 60
           MSTMKFCRECNNILYPKEDR++KILLYACRNCDHQEVAENNCVYRNEIHH+VGERTQVLQ
Sbjct: 1   MSTMKFCRECNNILYPKEDREQKILLYACRNCDHQEVAENNCVYRNEIHHSVGERTQVLQ 60

Query: 61  DVAADPTLPRTKAVRCAQCNHGEAVFFQATARGEEGMTLFFVCCNPNCGHRWRD 115
           DVAADPTLPRTK+VRCAQCNHGEAVFFQATARGEEGMTLFFVCCNPNCG+RWRD
Sbjct: 61  DVAADPTLPRTKSVRCAQCNHGEAVFFQATARGEEGMTLFFVCCNPNCGYRWRD 114

[Close]

BLAST of Lsi05G015850 vs. TrEMBL
Match: M5XGV0_PRUPE (DNA-directed RNA polymerase subunit OS=Prunus persica GN=PRUPE_ppa013594mg PE=3 SV=1)

HSP 1 Score: 243.0 bits (619), Expect = 1.7e-61
Identity = 106/114 (92.98%), Postives = 113/114 (99.12%), Query Frame = 1


Query: 1   MSTMKFCRECNNILYPKEDRDEKILLYACRNCDHQEVAENNCVYRNEIHHAVGERTQVLQ 60
           MSTMKFCRECNNILYPKED+D+KILLYACRNCDHQEVA+NNCVYRNEIHHAVGERTQ+LQ
Sbjct: 1   MSTMKFCRECNNILYPKEDKDQKILLYACRNCDHQEVADNNCVYRNEIHHAVGERTQILQ 60

Query: 61  DVAADPTLPRTKAVRCAQCNHGEAVFFQATARGEEGMTLFFVCCNPNCGHRWRD 115
           DVAADPTLPRTKAVRC++C HGEAVFFQATARGEEGMTLFFVCCNP+CGHRWRD
Sbjct: 61  DVAADPTLPRTKAVRCSECKHGEAVFFQATARGEEGMTLFFVCCNPSCGHRWRD 114

[Close]

BLAST of Lsi05G015850 vs. TrEMBL
Match: A0A0K9RIQ4_SPIOL (DNA-directed RNA polymerase subunit OS=Spinacia oleracea GN=SOVF_067320 PE=3 SV=1)

HSP 1 Score: 242.7 bits (618), Expect = 2.2e-61
Identity = 106/114 (92.98%), Postives = 112/114 (98.25%), Query Frame = 1


Query: 1   MSTMKFCRECNNILYPKEDRDEKILLYACRNCDHQEVAENNCVYRNEIHHAVGERTQVLQ 60
           MSTMKFCRECNNILYPKED+D+KIL YACRNCDHQE+AENNCVYRNEIHHAVGERTQVLQ
Sbjct: 1   MSTMKFCRECNNILYPKEDKDQKILQYACRNCDHQEIAENNCVYRNEIHHAVGERTQVLQ 60

Query: 61  DVAADPTLPRTKAVRCAQCNHGEAVFFQATARGEEGMTLFFVCCNPNCGHRWRD 115
           DVAADPTLPRTK+VRC QCNHGEAVFFQAT+RGEEGMTLFFVCCNP+CGHRWRD
Sbjct: 61  DVAADPTLPRTKSVRCTQCNHGEAVFFQATSRGEEGMTLFFVCCNPSCGHRWRD 114

[Close]

BLAST of Lsi05G015850 vs. TAIR10
Match: AT3G16980.1 (AT3G16980.1 RNA polymerases M/15 Kd subunit)

HSP 1 Score: 229.2 bits (583), Expect = 1.3e-60
Identity = 98/114 (85.96%), Postives = 110/114 (96.49%), Query Frame = 1


Query: 1   MSTMKFCRECNNILYPKEDRDEKILLYACRNCDHQEVAENNCVYRNEIHHAVGERTQVLQ 60
           MSTMKFCRECNNILYPKED+++KILLYACRNCDHQEVA+N+CVYRNE+HH+V ERTQ+L 
Sbjct: 1   MSTMKFCRECNNILYPKEDKEQKILLYACRNCDHQEVADNSCVYRNEVHHSVSERTQILT 60

Query: 61  DVAADPTLPRTKAVRCAQCNHGEAVFFQATARGEEGMTLFFVCCNPNCGHRWRD 115
           DVA+DPTLPRTKAVRC++C H EAVFFQATARGEEGMTLFFVCCNPNCGHRWR+
Sbjct: 61  DVASDPTLPRTKAVRCSKCQHREAVFFQATARGEEGMTLFFVCCNPNCGHRWRE 114

[Close]

BLAST of Lsi05G015850 vs. TAIR10
Match: AT4G16265.1 (AT4G16265.1 RNA polymerases M/15 Kd subunit)

HSP 1 Score: 228.4 bits (581), Expect = 2.2e-60
Identity = 97/114 (85.09%), Postives = 108/114 (94.74%), Query Frame = 1


Query: 1   MSTMKFCRECNNILYPKEDRDEKILLYACRNCDHQEVAENNCVYRNEIHHAVGERTQVLQ 60
           MSTMKFCRECNNILYPKED+++ ILLYACRNCDHQE A+NNCVYRNE+HH+V E+TQ+L 
Sbjct: 1   MSTMKFCRECNNILYPKEDKEQSILLYACRNCDHQEAADNNCVYRNEVHHSVSEQTQILS 60

Query: 61  DVAADPTLPRTKAVRCAQCNHGEAVFFQATARGEEGMTLFFVCCNPNCGHRWRD 115
           DVA+DPTLPRTKAVRCA+C HGEAVFFQATARGEEGMTLFFVCCNPNC HRWR+
Sbjct: 61  DVASDPTLPRTKAVRCAKCQHGEAVFFQATARGEEGMTLFFVCCNPNCSHRWRE 114

[Close]

BLAST of Lsi05G015850 vs. NCBI nr
Match: gi|659076932|ref|XP_008438942.1| (PREDICTED: DNA-directed RNA polymerases II, IV and V subunit 9A [Cucumis melo])

HSP 1 Score: 252.3 bits (643), Expect = 4.1e-64
Identity = 113/114 (99.12%), Postives = 114/114 (100.00%), Query Frame = 1


Query: 1   MSTMKFCRECNNILYPKEDRDEKILLYACRNCDHQEVAENNCVYRNEIHHAVGERTQVLQ 60
           MSTMKFCRECNNILYPKEDRDEKILLYACRNCDHQEVA+NNCVYRNEIHHAVGERTQVLQ
Sbjct: 1   MSTMKFCRECNNILYPKEDRDEKILLYACRNCDHQEVADNNCVYRNEIHHAVGERTQVLQ 60

Query: 61  DVAADPTLPRTKAVRCAQCNHGEAVFFQATARGEEGMTLFFVCCNPNCGHRWRD 115
           DVAADPTLPRTKAVRCAQCNHGEAVFFQATARGEEGMTLFFVCCNPNCGHRWRD
Sbjct: 61  DVAADPTLPRTKAVRCAQCNHGEAVFFQATARGEEGMTLFFVCCNPNCGHRWRD 114

[Close]

BLAST of Lsi05G015850 vs. NCBI nr
Match: gi|976898897|gb|KVH87674.1| (DNA-directed RNA polymerase, M/15kDa subunit [Cynara cardunculus var. scolymus])

HSP 1 Score: 247.7 bits (631), Expect = 1.0e-62
Identity = 110/114 (96.49%), Postives = 113/114 (99.12%), Query Frame = 1


Query: 1   MSTMKFCRECNNILYPKEDRDEKILLYACRNCDHQEVAENNCVYRNEIHHAVGERTQVLQ 60
           MSTMKFCRECNNILYPKED+D+KILLYACRNCDHQEVA+NNCVYRNEIHHAVGERTQVLQ
Sbjct: 1   MSTMKFCRECNNILYPKEDKDQKILLYACRNCDHQEVADNNCVYRNEIHHAVGERTQVLQ 60

Query: 61  DVAADPTLPRTKAVRCAQCNHGEAVFFQATARGEEGMTLFFVCCNPNCGHRWRD 115
           DVAADPTLPRTKAVRCAQC HGEAVFFQATARGEEGMTLFFVCCNPNCGHRWRD
Sbjct: 61  DVAADPTLPRTKAVRCAQCGHGEAVFFQATARGEEGMTLFFVCCNPNCGHRWRD 114

[Close]

BLAST of Lsi05G015850 vs. NCBI nr
Match: gi|225431922|ref|XP_002276956.1| (PREDICTED: DNA-directed RNA polymerases II, IV and V subunit 9A [Vitis vinifera])

HSP 1 Score: 246.1 bits (627), Expect = 2.9e-62
Identity = 109/114 (95.61%), Postives = 114/114 (100.00%), Query Frame = 1


Query: 1   MSTMKFCRECNNILYPKEDRDEKILLYACRNCDHQEVAENNCVYRNEIHHAVGERTQVLQ 60
           MSTMKFCRECNNILYPKEDR++KILLYACRNCDHQEVAENNCVYRNEIHH+VGERTQVLQ
Sbjct: 1   MSTMKFCRECNNILYPKEDREQKILLYACRNCDHQEVAENNCVYRNEIHHSVGERTQVLQ 60

Query: 61  DVAADPTLPRTKAVRCAQCNHGEAVFFQATARGEEGMTLFFVCCNPNCGHRWRD 115
           DVAADPTLPRTK+VRCAQCNHGEAVFFQATARGEEGMTLFFVCCNPNCG+RWRD
Sbjct: 61  DVAADPTLPRTKSVRCAQCNHGEAVFFQATARGEEGMTLFFVCCNPNCGYRWRD 114

[Close]

BLAST of Lsi05G015850 vs. NCBI nr
Match: gi|720038529|ref|XP_010268008.1| (PREDICTED: DNA-directed RNA polymerases II, IV and V subunit 9A [Nelumbo nucifera])

HSP 1 Score: 245.0 bits (624), Expect = 6.5e-62
Identity = 107/114 (93.86%), Postives = 114/114 (100.00%), Query Frame = 1


Query: 1   MSTMKFCRECNNILYPKEDRDEKILLYACRNCDHQEVAENNCVYRNEIHHAVGERTQVLQ 60
           MSTMKFCRECNNILYPKED+++KILLYACRNCDHQEVA+NNCVYRNEIHH+VGERTQVLQ
Sbjct: 1   MSTMKFCRECNNILYPKEDKEQKILLYACRNCDHQEVADNNCVYRNEIHHSVGERTQVLQ 60

Query: 61  DVAADPTLPRTKAVRCAQCNHGEAVFFQATARGEEGMTLFFVCCNPNCGHRWRD 115
           DVAADPTLPRTK+VRCAQCNHGEAVFFQAT+RGEEGMTLFFVCCNPNCGHRWRD
Sbjct: 61  DVAADPTLPRTKSVRCAQCNHGEAVFFQATSRGEEGMTLFFVCCNPNCGHRWRD 114

[Close]

BLAST of Lsi05G015850 vs. NCBI nr
Match: gi|1012109297|ref|XP_015959557.1| (PREDICTED: DNA-directed RNA polymerases II, IV and V subunit 9A [Arachis duranensis])

HSP 1 Score: 244.2 bits (622), Expect = 1.1e-61
Identity = 107/114 (93.86%), Postives = 113/114 (99.12%), Query Frame = 1


Query: 1   MSTMKFCRECNNILYPKEDRDEKILLYACRNCDHQEVAENNCVYRNEIHHAVGERTQVLQ 60
           MSTMKFCRECNNILYPKEDR++KILLYACRNCDHQEVA+NNCVYRNE+HH+VGERTQVLQ
Sbjct: 1   MSTMKFCRECNNILYPKEDREQKILLYACRNCDHQEVADNNCVYRNEVHHSVGERTQVLQ 60

Query: 61  DVAADPTLPRTKAVRCAQCNHGEAVFFQATARGEEGMTLFFVCCNPNCGHRWRD 115
           DVAADPTLPRTKAVRC QCNHGEAVFFQATARGEEGMTLFFVCCNPNCG+RWRD
Sbjct: 61  DVAADPTLPRTKAVRCVQCNHGEAVFFQATARGEEGMTLFFVCCNPNCGYRWRD 114

The following BLAST results are available for this feature:

BLAST of Lsi05G015850 vs. Swiss-Prot


Match Name	E-value	Identity	Description
RPB9A_ARATH	2.3e-59	85.96	DNA-directed RNA polymerases II, IV and V subunit 9A OS=Arabidopsis thaliana GN=...	[more]
RPB9B_ARATH	3.9e-59	85.09	DNA-directed RNA polymerases II, IV and V subunit 9B OS=Arabidopsis thaliana GN=...	[more]
RPB9_BOVIN	1.5e-37	58.04	DNA-directed RNA polymerase II subunit RPB9 OS=Bos taurus GN=POLR2I PE=1 SV=1	[more]
RPB9_HUMAN	1.5e-37	58.04	DNA-directed RNA polymerase II subunit RPB9 OS=Homo sapiens GN=POLR2I PE=1 SV=1	[more]
RPB9_MOUSE	1.5e-37	58.04	DNA-directed RNA polymerase II subunit RPB9 OS=Mus musculus GN=Polr2i PE=1 SV=1	[more]

BLAST of Lsi05G015850 vs. TrEMBL


Match Name	E-value	Identity	Description
A0A0A0L816_CUCSA	2.8e-64	99.12	DNA-directed RNA polymerase subunit OS=Cucumis sativus GN=Csa_3G166240 PE=3 SV=1	[more]
A0A103XBI1_CYNCS	7.0e-63	96.49	DNA-directed RNA polymerase subunit OS=Cynara cardunculus var. scolymus GN=Ccrd_...	[more]
A5AGZ7_VITVI	2.0e-62	95.61	DNA-directed RNA polymerase subunit OS=Vitis vinifera GN=VIT_05s0077g01170 PE=3 ...	[more]
M5XGV0_PRUPE	1.7e-61	92.98	DNA-directed RNA polymerase subunit OS=Prunus persica GN=PRUPE_ppa013594mg PE=3 ...	[more]
A0A0K9RIQ4_SPIOL	2.2e-61	92.98	DNA-directed RNA polymerase subunit OS=Spinacia oleracea GN=SOVF_067320 PE=3 SV=...	[more]

BLAST of Lsi05G015850 vs. TAIR10


Match Name	E-value	Identity	Description
AT3G16980.1	1.3e-60	85.96	RNA polymerases M/15 Kd subunit	[more]
AT4G16265.1	2.2e-60	85.09	RNA polymerases M/15 Kd subunit	[more]

BLAST of Lsi05G015850 vs. NCBI nr


Match Name	E-value	Identity	Description
gi\|659076932\|ref\|XP_008438942.1\|	4.1e-64	99.12	PREDICTED: DNA-directed RNA polymerases II, IV and V subunit 9A [Cucumis melo]	[more]
gi\|976898897\|gb\|KVH87674.1\|	1.0e-62	96.49	DNA-directed RNA polymerase, M/15kDa subunit [Cynara cardunculus var. scolymus]	[more]
gi\|225431922\|ref\|XP_002276956.1\|	2.9e-62	95.61	PREDICTED: DNA-directed RNA polymerases II, IV and V subunit 9A [Vitis vinifera]	[more]
gi\|720038529\|ref\|XP_010268008.1\|	6.5e-62	93.86	PREDICTED: DNA-directed RNA polymerases II, IV and V subunit 9A [Nelumbo nucifer...	[more]
gi\|1012109297\|ref\|XP_015959557.1\|	1.1e-61	93.86	PREDICTED: DNA-directed RNA polymerases II, IV and V subunit 9A [Arachis duranen...	[more]

Annotated Terms

The following terms have been associated with this gene:

Vocabulary: **Molecular Function**
Term	Definition
GO:0008270	zinc ion binding
GO:0003676	nucleic acid binding

Vocabulary: **Biological Process**
Term	Definition
GO:0006351	transcription, DNA-templated

Vocabulary: **INTERPRO**
Term	Definition
IPR019761	DNA-dir_RNA_pol-M_15_CS
IPR012164	Rpa12/Rpb9/Rpc10/TFS
IPR001529	DNA-dir_RNA_pol_M/15kDasu
IPR001222	Znf_TFIIS

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0001193	maintenance of transcriptional fidelity during DNA-templated transcription elongation from RNA polymerase II promoter
biological_process	GO:0006144	purine nucleobase metabolic process
biological_process	GO:0006206	pyrimidine nucleobase metabolic process
biological_process	GO:0006283	transcription-coupled nucleotide-excision repair
biological_process	GO:0006367	transcription initiation from RNA polymerase II promoter
biological_process	GO:0006351	transcription, DNA-templated
biological_process	GO:0006379	mRNA cleavage
cellular_component	GO:0005665	DNA-directed RNA polymerase II, core complex
cellular_component	GO:0005730	nucleolus
cellular_component	GO:0005575	cellular_component
molecular_function	GO:0003677	DNA binding
molecular_function	GO:0003899	DNA-directed RNA polymerase activity
molecular_function	GO:0008270	zinc ion binding
molecular_function	GO:0003676	nucleic acid binding
molecular_function	GO:0003674	molecular_function

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lsi05G015850.1	Lsi05G015850.1	mRNA

InterPro

Analysis Name: InterPro Annotations of Lagenaria siceraria
Date Performed: 2017-09-18


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001222	Zinc finger, TFIIS-type	PFAM	PF01096	TFIIS_C	coord: 75..113 score: 6.4
IPR001222	Zinc finger, TFIIS-type	SMART	SM00440	Cys4_2	coord: 74..114 score: 2.
IPR001222	Zinc finger, TFIIS-type	PROFILE	PS51133	ZF_TFIIS_2	coord: 72..113 score: 1
IPR001529	DNA-directed RNA polymerase, M/15kDa subunit	PFAM	PF02150	RNA_POL_M_15KD	coord: 4..36 score: 4.0
IPR001529	DNA-directed RNA polymerase, M/15kDa subunit	SMART	SM00661	rpol9cneu	coord: 5..58 score: 9.4
IPR012164	DNA-directed RNA polymerase subunit/transcription factor S	PIR	PIRSF005586	RNApol_RpoM	coord: 4..114 score: 4.3
IPR019761	DNA-directed RNA polymerase M, 15kDa subunit, conserved site	PROSITE	PS01030	RNA_POL_M_15KD	coord: 6..32 scor
None	No IPR available	GENE3D	G3DSA:2.20.25.10		coord: 1..46 score: 1.3E-25coord: 48..114 score: 2.4
None	No IPR available	PANTHER	PTHR11239	DNA-DIRECTED RNA POLYMERASE	coord: 1..114 score: 3.8
None	No IPR available	PANTHER	PTHR11239:SF15	SUBFAMILY NOT NAMED	coord: 1..114 score: 3.8
None	No IPR available	unknown	SSF57783	Zinc beta-ribbon	coord: 1..48 score: 1.7E-18coord: 52..114 score: 2.95

Synteny

The following gene(s) are orthologous to this gene:


Gene	Orthologue	Organism	Block
Lsi05G015850	Csa3G166240	Cucumber (Chinese Long) v2	culsiB199
Lsi05G015850	CSPI03G14900	Wild cucumber (PI 183967)	cpilsiB198
Lsi05G015850	Cucsa.252620	Cucumber (Gy14) v1	cgylsiB420
Lsi05G015850	CmaCh06G015570	Cucurbita maxima (Rimu)	cmalsiB757
Lsi05G015850	CmaCh14G015950	Cucurbita maxima (Rimu)	cmalsiB250
Lsi05G015850	Cla021782	Watermelon (97103) v1	lsiwmB362
Lsi05G015850	MELO3C006706	Melon (DHL92) v3.5.1	lsimeB369
Lsi05G015850	ClCG05G006460	Watermelon (Charleston Gray)	lsiwcgB333
Lsi05G015850	Cla97C05G088090	Watermelon (97103) v2	lsiwmbB330
Lsi05G015850	Bhi01G000773	Wax gourd	lsiwgoB432
Lsi05G015850	Cp4.1LG08g05130	Cucurbita pepo (Zucchini)	cpelsiB718
Lsi05G015850	Cp4.1LG03g11720	Cucurbita pepo (Zucchini)	cpelsiB512
Lsi05G015850	CsGy3G015010	Cucumber (Gy14) v2	cgyblsiB184
Lsi05G015850	Carg19630	Silver-seed gourd	carlsiB254
Lsi05G015850	Carg23740	Silver-seed gourd	carlsiB869

The following gene(s) are paralogous to this gene:

None

The following block(s) are covering this gene:


Gene	Organism	Block
Lsi05G015850	Cucumber (Chinese Long) v3	cuclsiB218
Lsi05G015850	Cucurbita moschata (Rifu)	cmolsiB238
Lsi05G015850	Cucurbita moschata (Rifu)	cmolsiB752
Lsi05G015850	Melon (DHL92) v3.6.1	lsimedB385