Lsi03G004870 (gene) Bottle gourd (USVL1VR-Ls) | Cucurbit Genomics Database (CuGenDB)

Overview


Name	Lsi03G004870
Type	gene
Organism	Lagenaria siceraria (Bottle gourd (USVL1VR-Ls))
Description	Calmodulin-like protein 1
Location	chr03 : 5564930 .. 5566194 (-)

Genome Viewer

Gene Expression

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGCCCATAAACCCAAATCTCCTTGACCTGCAATACAACGTCTCAAAGAAAAAGTTCCTTCGGGAACCTTCCCGGCTGTTCTCCTTCAAGGATCGGCAGAACTCCGGGCTCCGGCGGGCGCTGCAGCCGAGCACGGAGGAGATAAAGCAGATCTTCAACAAGTTCGACACAAACAGAGACGGGAGAATCTCAAAGCATGAATACAGGGGAATTCTGAAGGCGCTGGGGAGAGGTAATTCAATGGAAGAGGTGCAGAAGATTTTCAGGGCGGTAGATGCCGACGGCGATGGGTACATAAACTTGAACGAGTTTATGGAGGTTCACCGGAGCGGTGGCGGAGTTCAAGCGAGAGACGTTGAATTTGCTTTCAAGACGTTCGATTTGAATGGGGATCGGAAGATTAGTGCAGAGGAAGTGATGAGGGTGTTGAAGGGATTGGGGGAAAAGTGTAGCATTGAGGATTGCCGGCGGATGGTGAGGGCGGTGGATAGCGACGGCGACGGCATGGTGGACATTAATGAGTTTATGACCATGATGACTCGCTCTGCAAAGTATTAGAGGATGAGTCTTCTTAAGTTTGAGGGCTCTTTAATGGCGTTTCATGGGTTGTAAAATAGTTGAAATTTTTGTACGAGTGACCTTTTCACATGTCTCTTATCATTGATGACCCACTCCTCGCAATTTTATGAAATCTAATATACTGGTTAGGAACTTAGGCGAAGTATGTGGCCATTCCTAATGCGATAGTGCGCTTTGATGTAAGCATAATGTTAAATTTCATACATTTGTAAGAGACAGGTTATCAATCATAAAGAAGTCGTGAAAGGGTCACGGGTACAAAAAAAACTCTATAATTGTTGTGTAATGTAAATGCTTTGTGTTAATTTGGAGCTACAACTATTTCCATCCATCTACCAACTTAAATATAGCTAAACTGGCGTGAAGTTGTATTATCAACCTCGAGATATGAAACTTATCCTTTATTATGTATATTGGGAAAAATAGAAGAAAAAACACTATTTCCAACATAACACTAATGTTTTTTTTTTCTTTAGTTCAACAATGTTAGATAGTCAAATGAGATGGAACAATTAACCTTTAAGTTTTAATTGGTGCCTTATCCCTCGATTAAAAGAGTTATTATAACTTAATAAATAAAAAGAATATTGATTAATTAACAAAAGGTTTAATTATCATTTTTTTGTACATGCACTTATGGTTTTTGTTTCTTTTAGCCCATTTTGTTCAAGTCTGGTTCATTTTAG

mRNA sequence

ATGCCCATAAACCCAAATCTCCTTGACCTGCAATACAACGTCTCAAAGAAAAAGTTCCTTCGGGAACCTTCCCGGCTGTTCTCCTTCAAGGATCGGCAGAACTCCGGGCTCCGGCGGGCGCTGCAGCCGAGCACGGAGGAGATAAAGCAGATCTTCAACAAGTTCGACACAAACAGAGACGGGAGAATCTCAAAGCATGAATACAGGGGAATTCTGAAGGCGCTGGGGAGAGGTAATTCAATGGAAGAGGTGCAGAAGATTTTCAGGGCGGTAGATGCCGACGGCGATGGGTACATAAACTTGAACGAGTTTATGGAGGTTCACCGGAGCGGTGGCGGAGTTCAAGCGAGAGACGTTGAATTTGCTTTCAAGACGTTCGATTTGAATGGGGATCGGAAGATTAGTGCAGAGGAAGTGATGAGGGTGTTGAAGGGATTGGGGGAAAAGTGTAGCATTGAGGATTGCCGGCGGATGGTGAGGGCGGTGGATAGCGACGGCGACGGCATGCCCATTTTGTTCAAGTCTGGTTCATTTTAG

Coding sequence (CDS)

ATGCCCATAAACCCAAATCTCCTTGACCTGCAATACAACGTCTCAAAGAAAAAGTTCCTTCGGGAACCTTCCCGGCTGTTCTCCTTCAAGGATCGGCAGAACTCCGGGCTCCGGCGGGCGCTGCAGCCGAGCACGGAGGAGATAAAGCAGATCTTCAACAAGTTCGACACAAACAGAGACGGGAGAATCTCAAAGCATGAATACAGGGGAATTCTGAAGGCGCTGGGGAGAGGTAATTCAATGGAAGAGGTGCAGAAGATTTTCAGGGCGGTAGATGCCGACGGCGATGGGTACATAAACTTGAACGAGTTTATGGAGGTTCACCGGAGCGGTGGCGGAGTTCAAGCGAGAGACGTTGAATTTGCTTTCAAGACGTTCGATTTGAATGGGGATCGGAAGATTAGTGCAGAGGAAGTGATGAGGGTGTTGAAGGGATTGGGGGAAAAGTGTAGCATTGAGGATTGCCGGCGGATGGTGAGGGCGGTGGATAGCGACGGCGACGGCATGCCCATTTTGTTCAAGTCTGGTTCATTTTAG

Protein sequence

MPINPNLLDLQYNVSKKKFLREPSRLFSFKDRQNSGLRRALQPSTEEIKQIFNKFDTNRDGRISKHEYRGILKALGRGNSMEEVQKIFRAVDADGDGYINLNEFMEVHRSGGGVQARDVEFAFKTFDLNGDRKISAEEVMRVLKGLGEKCSIEDCRRMVRAVDSDGDGMPILFKSGSF

Homology

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BLAST of Lsi03G004870 vs. Swiss-Prot
Match: CML1_ARATH (Calmodulin-like protein 1 OS=Arabidopsis thaliana GN=CML1 PE=2 SV=1)

HSP 1 Score: 192.2 bits (487), Expect = 4.9e-48
Identity = 93/171 (54.39%), Postives = 133/171 (77.78%), Query Frame = 1


Query: 4   NPNLLDLQYNVSKKKFLREPSRLFSFKDRQNSGLRRA-----LQPSTEEIKQIFNKFDTN 63
           N + L+LQY +SK K LR+PSR+FS +DRQ+SGL         QPS  E++++F++FD +
Sbjct: 3   NVSFLELQYKLSKNKMLRKPSRMFS-RDRQSSGLSSPGPGGFSQPSVNEMRRVFSRFDLD 62

Query: 64  RDGRISKHEYRGILKALGRGNSMEEVQKIFRAVDADGDGYINLNEFMEVHRSGGGVQARD 123
           +DG+IS+ EY+ +L+ALG+  ++E+V KIF+AVD DGDG+I+  EF++ ++  GG+++ D
Sbjct: 63  KDGKISQTEYKVVLRALGQERAIEDVPKIFKAVDLDGDGFIDFREFIDAYKRSGGIRSSD 122

Query: 124 VEFAFKTFDLNGDRKISAEEVMRVLKGLGEKCSIEDCRRMVRAVDSDGDGM 170
           +  +F TFDLNGD KISAEEVM VL  LGE+CS+EDC RMVRAVD+DGDG+
Sbjct: 123 IRNSFWTFDLNGDGKISAEEVMSVLWKLGERCSLEDCNRMVRAVDADGDGL 172

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BLAST of Lsi03G004870 vs. Swiss-Prot
Match: CML10_ORYSJ (Probable calcium-binding protein CML10 OS=Oryza sativa subsp. japonica GN=CML10 PE=2 SV=1)

HSP 1 Score: 116.7 bits (291), Expect = 2.6e-25
Identity = 55/127 (43.31%), Postives = 85/127 (66.93%), Query Frame = 1


Query: 46  EEIKQIFNKFDTNRDGRISKHEYRGILKALGRGNSMEEVQKIFRAVDADGDGYINLNEFM 105
           EE++++F KFD N DGRIS+ E   + ++LG   + +E+ ++    DADGDG+I+L+EF 
Sbjct: 39  EEMERVFRKFDANGDGRISRSELGALFESLGHAATDDELARMMAEADADGDGFISLDEFA 98

Query: 106 EVHRSGGGVQA---RDVEFAFKTFDLNGDRKISAEEVMRVLKGLGEKCSIEDCRRMVRAV 165
            ++ +  G  A    D+  AF+ FD +G+  ISA E+ RVL GLGEK +++ CRRM+  V
Sbjct: 99  ALNATASGDAAAVEEDLRHAFRVFDADGNGTISAAELARVLHGLGEKATVQQCRRMIEGV 158

Query: 166 DSDGDGM 170
           D +GDG+
Sbjct: 159 DQNGDGL 165

[Close]

BLAST of Lsi03G004870 vs. Swiss-Prot
Match: CML23_ARATH (Probable calcium-binding protein CML23 OS=Arabidopsis thaliana GN=CML23 PE=2 SV=1)

HSP 1 Score: 115.9 bits (289), Expect = 4.4e-25
Identity = 57/129 (44.19%), Postives = 86/129 (66.67%), Query Frame = 1


Query: 44  STEEIKQIFNKFDTNRDGRISKHEYRGILKALGRGNSMEEVQKIFRAVDADGDGYINLNE 103
           S E+IK++F +FD N DG+IS  E + ++ AL    S EE + + +  D DG+G+I+L+E
Sbjct: 12  SMEDIKKVFQRFDKNNDGKISIDELKDVIGALSPNASQEETKAMMKEFDLDGNGFIDLDE 71

Query: 104 FMEVHR----SGGGVQARDVEFAFKTFDLNGDRKISAEEVMRVLKGLGEKCSIEDCRRMV 163
           F+ + +    S      RD++ AF  +DL+ + +ISA E+  V+K LGEKCSI+DC+RM+
Sbjct: 72  FVALFQISDQSSNNSAIRDLKEAFDLYDLDRNGRISANELHSVMKNLGEKCSIQDCQRMI 131

Query: 164 RAVDSDGDG 169
             VDSDGDG
Sbjct: 132 NKVDSDGDG 140

[Close]

BLAST of Lsi03G004870 vs. Swiss-Prot
Match: CML24_ARATH (Calcium-binding protein CML24 OS=Arabidopsis thaliana GN=CML24 PE=2 SV=2)

HSP 1 Score: 115.2 bits (287), Expect = 7.6e-25
Identity = 58/140 (41.43%), Postives = 94/140 (67.14%), Query Frame = 1


Query: 35  SGLRRALQPSTEEIKQIFNKFDTNRDGRISKHEYRGILKALGRGNSMEEVQKIFRAVDAD 94
           +G+ R+   S ++IK++F +FD N DG+IS  E + +++AL    S EE   + +  D D
Sbjct: 5   NGVVRSCLGSMDDIKKVFQRFDKNGDGKISVDELKEVIRALSPTASPEETVTMMKQFDLD 64

Query: 95  GDGYINLNEFMEVHR---SGGGVQARDV---EFAFKTFDLNGDRKISAEEVMRVLKGLGE 154
           G+G+I+L+EF+ + +    GGG    DV   + AF+ +DL+G+ +ISA+E+  V+K LGE
Sbjct: 65  GNGFIDLDEFVALFQIGIGGGGNNRNDVSDLKEAFELYDLDGNGRISAKELHSVMKNLGE 124

Query: 155 KCSIEDCRRMVRAVDSDGDG 169
           KCS++DC++M+  VD DGDG
Sbjct: 125 KCSVQDCKKMISKVDIDGDG 144

[Close]

BLAST of Lsi03G004870 vs. Swiss-Prot
Match: CML25_ARATH (Probable calcium-binding protein CML25 OS=Arabidopsis thaliana GN=CML25 PE=2 SV=1)

HSP 1 Score: 110.5 bits (275), Expect = 1.9e-23
Identity = 54/132 (40.91%), Postives = 82/132 (62.12%), Query Frame = 1


Query: 39  RALQPSTEEIKQIFNKFDTNRDGRISKHEYRGILKALGRGNSMEEVQKIFRAVDADGDGY 98
           R+ +    E++ +F KFD N DG+IS  E   I+ +LG     EE++K    +D  GDGY
Sbjct: 29  RSGKTEIRELEAVFKKFDVNGDGKISSKELGAIMTSLGHEVPEEELEKAITEIDRKGDGY 88

Query: 99  INLNEFMEVHRSGGGVQ--ARDVEFAFKTFDLNGDRKISAEEVMRVLKGLGEKCSIEDCR 158
           IN  EF+E++  G        +++ AF  +D++G+  ISAEE+  VL+ LG++CSI +CR
Sbjct: 89  INFEEFVELNTKGMDQNDVLENLKDAFSVYDIDGNGSISAEELHEVLRSLGDECSIAECR 148

Query: 159 RMVRAVDSDGDG 169
           +M+  VD DGDG
Sbjct: 149 KMIGGVDKDGDG 160

[Close]

BLAST of Lsi03G004870 vs. TrEMBL
Match: A0A0A0K804_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_7G073720 PE=4 SV=1)

HSP 1 Score: 327.8 bits (839), Expect = 8.3e-87
Identity = 161/169 (95.27%), Postives = 168/169 (99.41%), Query Frame = 1


Query: 1   MPINPNLLDLQYNVSKKKFLREPSRLFSFKDRQNSGLRRALQPSTEEIKQIFNKFDTNRD 60
           MPINPN L+LQYNVSKKKFLREPSRLFSFKDRQ+SGLRRALQPS+EEIKQIFNKFDTN+D
Sbjct: 1   MPINPNFLELQYNVSKKKFLREPSRLFSFKDRQSSGLRRALQPSSEEIKQIFNKFDTNKD 60

Query: 61  GRISKHEYRGILKALGRGNSMEEVQKIFRAVDADGDGYINLNEFMEVHRSGGGVQARDVE 120
           GRISKHEYRGILKALGRGNSMEEVQKIFRAVD+DGDGYINLNEFMEVHRSGGGVQA++VE
Sbjct: 61  GRISKHEYRGILKALGRGNSMEEVQKIFRAVDSDGDGYINLNEFMEVHRSGGGVQAKEVE 120

Query: 121 FAFKTFDLNGDRKISAEEVMRVLKGLGEKCSIEDCRRMVRAVDSDGDGM 170
           FAFKTFDLNGDRKISAEEVMRVLKGLGEKCSIEDCRRMVRAVDSDGDGM
Sbjct: 121 FAFKTFDLNGDRKISAEEVMRVLKGLGEKCSIEDCRRMVRAVDSDGDGM 169

[Close]

BLAST of Lsi03G004870 vs. TrEMBL
Match: M5VNS5_PRUPE (Uncharacterized protein OS=Prunus persica GN=PRUPE_ppa011990mg PE=4 SV=1)

HSP 1 Score: 229.6 bits (584), Expect = 3.1e-57
Identity = 105/164 (64.02%), Postives = 137/164 (83.54%), Query Frame = 1


Query: 6   NLLDLQYNVSKKKFLREPSRLFSFKDRQNSGLRRALQPSTEEIKQIFNKFDTNRDGRISK 65
           N LD QYN+SK+KFLR+PS +FSF+DRQNSG+  + +P+  E+K++F+KFD+N+DG+IS+
Sbjct: 5   NFLDFQYNLSKRKFLRKPSHVFSFRDRQNSGVSPSFEPNPNEMKRVFDKFDSNKDGKISQ 64

Query: 66  HEYRGILKALGRGNSMEEVQKIFRAVDADGDGYINLNEFMEVHRSGGGVQARDVEFAFKT 125
            EY+ IL+ALG+GN + EV KIFR  D DGDG+IN  EFMEVH+ GGG++  D++ AF+T
Sbjct: 65  PEYKAILRALGKGNMVGEVPKIFRIADLDGDGFINFKEFMEVHKKGGGIRTIDIQNAFRT 124

Query: 126 FDLNGDRKISAEEVMRVLKGLGEKCSIEDCRRMVRAVDSDGDGM 170
           FDLNGD KISAEEVM VL  LGE CS+EDC+RMVRAVD+DGDG+
Sbjct: 125 FDLNGDGKISAEEVMEVLGRLGESCSLEDCQRMVRAVDTDGDGV 168

[Close]

BLAST of Lsi03G004870 vs. TrEMBL
Match: A0A0B2RCG8_GLYSO (Calmodulin-like protein 1 OS=Glycine soja GN=glysoja_020368 PE=4 SV=1)

HSP 1 Score: 215.7 bits (548), Expect = 4.6e-53
Identity = 104/165 (63.03%), Postives = 132/165 (80.00%), Query Frame = 1


Query: 6   NLLDLQYNVSKKKFLREPSRLFSFKDRQNSGLRRA-LQPSTEEIKQIFNKFDTNRDGRIS 65
           + LD QYN+S++K+LR+PSRLFS +DRQNSGL+   LQP+ +E+KQ+F+KFD+N+DG+IS
Sbjct: 97  SFLDFQYNLSRRKYLRKPSRLFS-RDRQNSGLKGVFLQPNADEMKQVFDKFDSNKDGKIS 156

Query: 66  KHEYRGILKALGRGNSMEEVQKIFRAVDADGDGYINLNEFMEVHRSGGGVQARDVEFAFK 125
           + EY+  +KALG G+S+ EV  IFR VD DGDG+IN  EFME    GGGV+  D+  AF+
Sbjct: 157 QQEYKATMKALGMGDSVHEVPNIFRVVDLDGDGFINFKEFMEAQNKGGGVRTMDIHSAFR 216

Query: 126 TFDLNGDRKISAEEVMRVLKGLGEKCSIEDCRRMVRAVDSDGDGM 170
           TFD NGD +ISAEEV   L  LGE+CSIEDCRRMVRAVD+DGDGM
Sbjct: 217 TFDRNGDGRISAEEVKETLGRLGERCSIEDCRRMVRAVDTDGDGM 260

[Close]

BLAST of Lsi03G004870 vs. TrEMBL
Match: C6TAX4_SOYBN (Uncharacterized protein OS=Glycine max GN=GLYMA_07G212000 PE=2 SV=1)

HSP 1 Score: 215.7 bits (548), Expect = 4.6e-53
Identity = 104/165 (63.03%), Postives = 132/165 (80.00%), Query Frame = 1


Query: 6   NLLDLQYNVSKKKFLREPSRLFSFKDRQNSGLRRA-LQPSTEEIKQIFNKFDTNRDGRIS 65
           + LD QYN+S++K+LR+PSRLFS +DRQNSGL+   LQP+ +E+KQ+F+KFD+N+DG+IS
Sbjct: 5   SFLDFQYNLSRRKYLRKPSRLFS-RDRQNSGLKGVFLQPNADEMKQVFDKFDSNKDGKIS 64

Query: 66  KHEYRGILKALGRGNSMEEVQKIFRAVDADGDGYINLNEFMEVHRSGGGVQARDVEFAFK 125
           + EY+  +KALG G+S+ EV  IFR VD DGDG+IN  EFME    GGGV+  D+  AF+
Sbjct: 65  QQEYKATMKALGMGDSVHEVPNIFRVVDLDGDGFINFKEFMEAQNKGGGVRTMDIHSAFR 124

Query: 126 TFDLNGDRKISAEEVMRVLKGLGEKCSIEDCRRMVRAVDSDGDGM 170
           TFD NGD +ISAEEV   L  LGE+CSIEDCRRMVRAVD+DGDGM
Sbjct: 125 TFDRNGDGRISAEEVKETLGRLGERCSIEDCRRMVRAVDTDGDGM 168

[Close]

BLAST of Lsi03G004870 vs. TrEMBL
Match: B9HS04_POPTR (Calmodulin-related family protein OS=Populus trichocarpa GN=POPTR_0009s10530g PE=4 SV=1)

HSP 1 Score: 214.5 bits (545), Expect = 1.0e-52
Identity = 98/166 (59.04%), Postives = 133/166 (80.12%), Query Frame = 1


Query: 4   NPNLLDLQYNVSKKKFLREPSRLFSFKDRQNSGLRRALQPSTEEIKQIFNKFDTNRDGRI 63
           N + L+ QY +S+ KFLR+PSRLFS +DRQ+SG+    QP+ +E++Q+F+KFD+N+DG+I
Sbjct: 3   NLSFLEFQYKLSRNKFLRKPSRLFSSRDRQSSGMLSTFQPNMKEMRQVFDKFDSNKDGKI 62

Query: 64  SKHEYRGILKALGRGNSMEEVQKIFRAVDADGDGYINLNEFMEVHRSGGGVQARDVEFAF 123
           S+ EY+  L+ALG+GN + EV KIF+ VD DGDG+I+  EF+E  + GGG++  D++ AF
Sbjct: 63  SQQEYKDTLRALGQGNMLGEVPKIFQVVDLDGDGFIDFKEFVEAQKKGGGIRTTDIQTAF 122

Query: 124 KTFDLNGDRKISAEEVMRVLKGLGEKCSIEDCRRMVRAVDSDGDGM 170
           +TFD NGD KISAEEVM VL+ LGE+CS+EDCRRMV AVD DGDGM
Sbjct: 123 QTFDSNGDGKISAEEVMEVLRRLGERCSLEDCRRMVNAVDIDGDGM 168

[Close]

BLAST of Lsi03G004870 vs. TAIR10
Match: AT2G15680.1 (AT2G15680.1 Calcium-binding EF-hand family protein)

HSP 1 Score: 192.2 bits (487), Expect = 2.7e-49
Identity = 93/171 (54.39%), Postives = 133/171 (77.78%), Query Frame = 1


Query: 4   NPNLLDLQYNVSKKKFLREPSRLFSFKDRQNSGLRRA-----LQPSTEEIKQIFNKFDTN 63
           N + L+LQY +SK K LR+PSR+FS +DRQ+SGL         QPS  E++++F++FD +
Sbjct: 3   NVSFLELQYKLSKNKMLRKPSRMFS-RDRQSSGLSSPGPGGFSQPSVNEMRRVFSRFDLD 62

Query: 64  RDGRISKHEYRGILKALGRGNSMEEVQKIFRAVDADGDGYINLNEFMEVHRSGGGVQARD 123
           +DG+IS+ EY+ +L+ALG+  ++E+V KIF+AVD DGDG+I+  EF++ ++  GG+++ D
Sbjct: 63  KDGKISQTEYKVVLRALGQERAIEDVPKIFKAVDLDGDGFIDFREFIDAYKRSGGIRSSD 122

Query: 124 VEFAFKTFDLNGDRKISAEEVMRVLKGLGEKCSIEDCRRMVRAVDSDGDGM 170
           +  +F TFDLNGD KISAEEVM VL  LGE+CS+EDC RMVRAVD+DGDG+
Sbjct: 123 IRNSFWTFDLNGDGKISAEEVMSVLWKLGERCSLEDCNRMVRAVDADGDGL 172

[Close]

BLAST of Lsi03G004870 vs. TAIR10
Match: AT1G66400.1 (AT1G66400.1 calmodulin like 23)

HSP 1 Score: 115.9 bits (289), Expect = 2.5e-26
Identity = 57/129 (44.19%), Postives = 86/129 (66.67%), Query Frame = 1


Query: 44  STEEIKQIFNKFDTNRDGRISKHEYRGILKALGRGNSMEEVQKIFRAVDADGDGYINLNE 103
           S E+IK++F +FD N DG+IS  E + ++ AL    S EE + + +  D DG+G+I+L+E
Sbjct: 12  SMEDIKKVFQRFDKNNDGKISIDELKDVIGALSPNASQEETKAMMKEFDLDGNGFIDLDE 71

Query: 104 FMEVHR----SGGGVQARDVEFAFKTFDLNGDRKISAEEVMRVLKGLGEKCSIEDCRRMV 163
           F+ + +    S      RD++ AF  +DL+ + +ISA E+  V+K LGEKCSI+DC+RM+
Sbjct: 72  FVALFQISDQSSNNSAIRDLKEAFDLYDLDRNGRISANELHSVMKNLGEKCSIQDCQRMI 131

Query: 164 RAVDSDGDG 169
             VDSDGDG
Sbjct: 132 NKVDSDGDG 140

[Close]

BLAST of Lsi03G004870 vs. TAIR10
Match: AT5G37770.1 (AT5G37770.1 EF hand calcium-binding protein family)

HSP 1 Score: 115.2 bits (287), Expect = 4.3e-26
Identity = 58/140 (41.43%), Postives = 94/140 (67.14%), Query Frame = 1


Query: 35  SGLRRALQPSTEEIKQIFNKFDTNRDGRISKHEYRGILKALGRGNSMEEVQKIFRAVDAD 94
           +G+ R+   S ++IK++F +FD N DG+IS  E + +++AL    S EE   + +  D D
Sbjct: 5   NGVVRSCLGSMDDIKKVFQRFDKNGDGKISVDELKEVIRALSPTASPEETVTMMKQFDLD 64

Query: 95  GDGYINLNEFMEVHR---SGGGVQARDV---EFAFKTFDLNGDRKISAEEVMRVLKGLGE 154
           G+G+I+L+EF+ + +    GGG    DV   + AF+ +DL+G+ +ISA+E+  V+K LGE
Sbjct: 65  GNGFIDLDEFVALFQIGIGGGGNNRNDVSDLKEAFELYDLDGNGRISAKELHSVMKNLGE 124

Query: 155 KCSIEDCRRMVRAVDSDGDG 169
           KCS++DC++M+  VD DGDG
Sbjct: 125 KCSVQDCKKMISKVDIDGDG 144

[Close]

BLAST of Lsi03G004870 vs. TAIR10
Match: AT1G24620.1 (AT1G24620.1 EF hand calcium-binding protein family)

HSP 1 Score: 110.5 bits (275), Expect = 1.0e-24
Identity = 54/132 (40.91%), Postives = 82/132 (62.12%), Query Frame = 1


Query: 39  RALQPSTEEIKQIFNKFDTNRDGRISKHEYRGILKALGRGNSMEEVQKIFRAVDADGDGY 98
           R+ +    E++ +F KFD N DG+IS  E   I+ +LG     EE++K    +D  GDGY
Sbjct: 29  RSGKTEIRELEAVFKKFDVNGDGKISSKELGAIMTSLGHEVPEEELEKAITEIDRKGDGY 88

Query: 99  INLNEFMEVHRSGGGVQ--ARDVEFAFKTFDLNGDRKISAEEVMRVLKGLGEKCSIEDCR 158
           IN  EF+E++  G        +++ AF  +D++G+  ISAEE+  VL+ LG++CSI +CR
Sbjct: 89  INFEEFVELNTKGMDQNDVLENLKDAFSVYDIDGNGSISAEELHEVLRSLGDECSIAECR 148

Query: 159 RMVRAVDSDGDG 169
           +M+  VD DGDG
Sbjct: 149 KMIGGVDKDGDG 160

[Close]

BLAST of Lsi03G004870 vs. TAIR10
Match: AT1G18210.1 (AT1G18210.1 Calcium-binding EF-hand family protein)

HSP 1 Score: 110.2 bits (274), Expect = 1.4e-24
Identity = 55/123 (44.72%), Postives = 80/123 (65.04%), Query Frame = 1


Query: 46  EEIKQIFNKFDTNRDGRISKHEYRGILKALGRGNSMEEVQKIFRAVDADGDGYINLNEFM 105
           EE+K++F++FD+N DG+IS  E  G+ KA+G   +  E+ ++   VD D DGYINL+EF 
Sbjct: 22  EELKKVFDQFDSNGDGKISVLELGGVFKAMGTSYTETELNRVLEEVDTDRDGYINLDEFS 81

Query: 106 EVHRSGGGVQARDVEFAFKTFDLNGDRKISAEEVMRVLKGLGEKCSIEDCRRMVRAVDSD 165
            + RS     A ++  AF  +D + +  ISA E+ +VL  LG  CS+EDC RM+  VD+D
Sbjct: 82  TLCRSSS--SAAEIRDAFDLYDQDKNGLISASELHQVLNRLGMSCSVEDCTRMIGPVDAD 141

Query: 166 GDG 169
           GDG
Sbjct: 142 GDG 142

[Close]

BLAST of Lsi03G004870 vs. NCBI nr
Match: gi|778725125|ref|XP_004137114.2| (PREDICTED: calmodulin-like protein 1 [Cucumis sativus])

HSP 1 Score: 327.8 bits (839), Expect = 1.2e-86
Identity = 161/169 (95.27%), Postives = 168/169 (99.41%), Query Frame = 1


Query: 1   MPINPNLLDLQYNVSKKKFLREPSRLFSFKDRQNSGLRRALQPSTEEIKQIFNKFDTNRD 60
           MPINPN L+LQYNVSKKKFLREPSRLFSFKDRQ+SGLRRALQPS+EEIKQIFNKFDTN+D
Sbjct: 1   MPINPNFLELQYNVSKKKFLREPSRLFSFKDRQSSGLRRALQPSSEEIKQIFNKFDTNKD 60

Query: 61  GRISKHEYRGILKALGRGNSMEEVQKIFRAVDADGDGYINLNEFMEVHRSGGGVQARDVE 120
           GRISKHEYRGILKALGRGNSMEEVQKIFRAVD+DGDGYINLNEFMEVHRSGGGVQA++VE
Sbjct: 61  GRISKHEYRGILKALGRGNSMEEVQKIFRAVDSDGDGYINLNEFMEVHRSGGGVQAKEVE 120

Query: 121 FAFKTFDLNGDRKISAEEVMRVLKGLGEKCSIEDCRRMVRAVDSDGDGM 170
           FAFKTFDLNGDRKISAEEVMRVLKGLGEKCSIEDCRRMVRAVDSDGDGM
Sbjct: 121 FAFKTFDLNGDRKISAEEVMRVLKGLGEKCSIEDCRRMVRAVDSDGDGM 169

[Close]

BLAST of Lsi03G004870 vs. NCBI nr
Match: gi|659109907|ref|XP_008454944.1| (PREDICTED: calmodulin-like protein 1 [Cucumis melo])

HSP 1 Score: 326.6 bits (836), Expect = 2.6e-86
Identity = 161/169 (95.27%), Postives = 167/169 (98.82%), Query Frame = 1


Query: 1   MPINPNLLDLQYNVSKKKFLREPSRLFSFKDRQNSGLRRALQPSTEEIKQIFNKFDTNRD 60
           MPINPN LDLQYNVSKKKFLREPSRLFSFKDRQ+SGLRRALQPS EEIKQIFNKFDTN+D
Sbjct: 1   MPINPNFLDLQYNVSKKKFLREPSRLFSFKDRQSSGLRRALQPSLEEIKQIFNKFDTNKD 60

Query: 61  GRISKHEYRGILKALGRGNSMEEVQKIFRAVDADGDGYINLNEFMEVHRSGGGVQARDVE 120
           GRISKHEYRGILKALGRG+SMEEVQKIFRAVD+DGDGYINLNEFMEVHRSGGGVQA++VE
Sbjct: 61  GRISKHEYRGILKALGRGSSMEEVQKIFRAVDSDGDGYINLNEFMEVHRSGGGVQAKEVE 120

Query: 121 FAFKTFDLNGDRKISAEEVMRVLKGLGEKCSIEDCRRMVRAVDSDGDGM 170
           FAFKTFDLNGDRKISAEEVMRVLKGLGEKCSIEDCRRMVRAVDSDGDGM
Sbjct: 121 FAFKTFDLNGDRKISAEEVMRVLKGLGEKCSIEDCRRMVRAVDSDGDGM 169

[Close]

BLAST of Lsi03G004870 vs. NCBI nr
Match: gi|595798018|ref|XP_007201342.1| (hypothetical protein PRUPE_ppa011990mg [Prunus persica])

HSP 1 Score: 229.6 bits (584), Expect = 4.4e-57
Identity = 105/164 (64.02%), Postives = 137/164 (83.54%), Query Frame = 1


Query: 6   NLLDLQYNVSKKKFLREPSRLFSFKDRQNSGLRRALQPSTEEIKQIFNKFDTNRDGRISK 65
           N LD QYN+SK+KFLR+PS +FSF+DRQNSG+  + +P+  E+K++F+KFD+N+DG+IS+
Sbjct: 5   NFLDFQYNLSKRKFLRKPSHVFSFRDRQNSGVSPSFEPNPNEMKRVFDKFDSNKDGKISQ 64

Query: 66  HEYRGILKALGRGNSMEEVQKIFRAVDADGDGYINLNEFMEVHRSGGGVQARDVEFAFKT 125
            EY+ IL+ALG+GN + EV KIFR  D DGDG+IN  EFMEVH+ GGG++  D++ AF+T
Sbjct: 65  PEYKAILRALGKGNMVGEVPKIFRIADLDGDGFINFKEFMEVHKKGGGIRTIDIQNAFRT 124

Query: 126 FDLNGDRKISAEEVMRVLKGLGEKCSIEDCRRMVRAVDSDGDGM 170
           FDLNGD KISAEEVM VL  LGE CS+EDC+RMVRAVD+DGDG+
Sbjct: 125 FDLNGDGKISAEEVMEVLGRLGESCSLEDCQRMVRAVDTDGDGV 168

[Close]

BLAST of Lsi03G004870 vs. NCBI nr
Match: gi|764562626|ref|XP_011461491.1| (PREDICTED: calmodulin-like protein 1 [Fragaria vesca subsp. vesca])

HSP 1 Score: 229.6 bits (584), Expect = 4.4e-57
Identity = 107/166 (64.46%), Postives = 137/166 (82.53%), Query Frame = 1


Query: 4   NPNLLDLQYNVSKKKFLREPSRLFSFKDRQNSGLRRALQPSTEEIKQIFNKFDTNRDGRI 63
           N + LD QYN+SK+KFLR P+RLFS +DRQNSGL    +P+  E+K++F KFDTN+DG+I
Sbjct: 3   NMSFLDFQYNLSKRKFLRTPTRLFSSRDRQNSGLTPGFEPNLNEMKRVFEKFDTNKDGKI 62

Query: 64  SKHEYRGILKALGRGNSMEEVQKIFRAVDADGDGYINLNEFMEVHRSGGGVQARDVEFAF 123
           S+ +YR IL+ALG+G+ + EV KIF+ VD DGDG+IN  EFMEVH+  GGV+  D++ AF
Sbjct: 63  SQQDYRAILRALGKGSMVGEVPKIFKLVDLDGDGFINFKEFMEVHKKSGGVKTIDIQNAF 122

Query: 124 KTFDLNGDRKISAEEVMRVLKGLGEKCSIEDCRRMVRAVDSDGDGM 170
           +TFDLNGD KISAEEVM VLK LGE+CS+E+C+RMVRAVD+DGDGM
Sbjct: 123 QTFDLNGDGKISAEEVMEVLKRLGERCSLEECQRMVRAVDTDGDGM 168

[Close]

BLAST of Lsi03G004870 vs. NCBI nr
Match: gi|645261897|ref|XP_008236514.1| (PREDICTED: calmodulin-like protein 1 [Prunus mume])

HSP 1 Score: 228.8 bits (582), Expect = 7.5e-57
Identity = 104/164 (63.41%), Postives = 136/164 (82.93%), Query Frame = 1


Query: 6   NLLDLQYNVSKKKFLREPSRLFSFKDRQNSGLRRALQPSTEEIKQIFNKFDTNRDGRISK 65
           N LD Q+N+SK+KFLR+PS + SF+DRQNSGL  + +P+  E+K +F KFD+N+DG+IS+
Sbjct: 5   NFLDFQFNLSKRKFLRKPSHVCSFRDRQNSGLSPSFEPNPNEMKIVFEKFDSNKDGKISQ 64

Query: 66  HEYRGILKALGRGNSMEEVQKIFRAVDADGDGYINLNEFMEVHRSGGGVQARDVEFAFKT 125
            EY+ IL+ALG+GN++ EV KIFR  D DGDG+IN  EFMEVH+ GGG++  D++ AF+T
Sbjct: 65  EEYKAILRALGKGNTVREVPKIFRIADLDGDGFINFKEFMEVHKKGGGIRTMDIQNAFRT 124

Query: 126 FDLNGDRKISAEEVMRVLKGLGEKCSIEDCRRMVRAVDSDGDGM 170
           FDLNGD KISAEEVM VL+ LGE CS+EDC+RMVRAVD+DGDG+
Sbjct: 125 FDLNGDGKISAEEVMEVLRRLGESCSLEDCQRMVRAVDTDGDGV 168

The following BLAST results are available for this feature:

BLAST of Lsi03G004870 vs. Swiss-Prot


Match Name	E-value	Identity	Description
CML1_ARATH	4.9e-48	54.39	Calmodulin-like protein 1 OS=Arabidopsis thaliana GN=CML1 PE=2 SV=1	[more]
CML10_ORYSJ	2.6e-25	43.31	Probable calcium-binding protein CML10 OS=Oryza sativa subsp. japonica GN=CML10 ...	[more]
CML23_ARATH	4.4e-25	44.19	Probable calcium-binding protein CML23 OS=Arabidopsis thaliana GN=CML23 PE=2 SV=...	[more]
CML24_ARATH	7.6e-25	41.43	Calcium-binding protein CML24 OS=Arabidopsis thaliana GN=CML24 PE=2 SV=2	[more]
CML25_ARATH	1.9e-23	40.91	Probable calcium-binding protein CML25 OS=Arabidopsis thaliana GN=CML25 PE=2 SV=...	[more]

BLAST of Lsi03G004870 vs. TrEMBL


Match Name	E-value	Identity	Description
A0A0A0K804_CUCSA	8.3e-87	95.27	Uncharacterized protein OS=Cucumis sativus GN=Csa_7G073720 PE=4 SV=1	[more]
M5VNS5_PRUPE	3.1e-57	64.02	Uncharacterized protein OS=Prunus persica GN=PRUPE_ppa011990mg PE=4 SV=1	[more]
A0A0B2RCG8_GLYSO	4.6e-53	63.03	Calmodulin-like protein 1 OS=Glycine soja GN=glysoja_020368 PE=4 SV=1	[more]
C6TAX4_SOYBN	4.6e-53	63.03	Uncharacterized protein OS=Glycine max GN=GLYMA_07G212000 PE=2 SV=1	[more]
B9HS04_POPTR	1.0e-52	59.04	Calmodulin-related family protein OS=Populus trichocarpa GN=POPTR_0009s10530g PE...	[more]

BLAST of Lsi03G004870 vs. TAIR10


Match Name	E-value	Identity	Description
AT2G15680.1	2.7e-49	54.39	Calcium-binding EF-hand family protein	[more]
AT1G66400.1	2.5e-26	44.19	calmodulin like 23	[more]
AT5G37770.1	4.3e-26	41.43	EF hand calcium-binding protein family	[more]
AT1G24620.1	1.0e-24	40.91	EF hand calcium-binding protein family	[more]
AT1G18210.1	1.4e-24	44.72	Calcium-binding EF-hand family protein	[more]

BLAST of Lsi03G004870 vs. NCBI nr


Match Name	E-value	Identity	Description
gi\|778725125\|ref\|XP_004137114.2\|	1.2e-86	95.27	PREDICTED: calmodulin-like protein 1 [Cucumis sativus]	[more]
gi\|659109907\|ref\|XP_008454944.1\|	2.6e-86	95.27	PREDICTED: calmodulin-like protein 1 [Cucumis melo]	[more]
gi\|595798018\|ref\|XP_007201342.1\|	4.4e-57	64.02	hypothetical protein PRUPE_ppa011990mg [Prunus persica]	[more]
gi\|764562626\|ref\|XP_011461491.1\|	4.4e-57	64.46	PREDICTED: calmodulin-like protein 1 [Fragaria vesca subsp. vesca]	[more]
gi\|645261897\|ref\|XP_008236514.1\|	7.5e-57	63.41	PREDICTED: calmodulin-like protein 1 [Prunus mume]	[more]

Annotated Terms

The following terms have been associated with this gene:

Vocabulary: **Molecular Function**
Term	Definition
GO:0005509	calcium ion binding

Vocabulary: **INTERPRO**
Term	Definition
IPR018247	EF_Hand_1_Ca_BS
IPR011992	EF-hand-dom_pair
IPR002048	EF_hand_dom

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0008150	biological_process
cellular_component	GO:0005739	mitochondrion
cellular_component	GO:0005575	cellular_component
molecular_function	GO:0005509	calcium ion binding

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lsi03G004870.1	Lsi03G004870.1	mRNA

InterPro

Analysis Name: InterPro Annotations of Lagenaria siceraria
Date Performed: 2017-09-18


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR002048	EF-hand domain	PFAM	PF13499	EF-hand_7	coord: 46..106 score: 1.1
IPR002048	EF-hand domain	PFAM	PF13833	EF-hand_8	coord: 133..169 score: 0.
IPR002048	EF-hand domain	SMART	SM00054	efh_1	coord: 47..75 score: 7.2E-7coord: 118..146 score: 0.0016coord: 83..111 score: 3.
IPR002048	EF-hand domain	PROFILE	PS50222	EF_HAND_2	coord: 79..114 score: 14.541coord: 118..149 score: 12.533coord: 43..78 score: 15
IPR011992	EF-hand domain pair	GENE3D	G3DSA:1.10.238.10		coord: 45..132 score: 1.3E-25coord: 133..169 score: 6.2
IPR011992	EF-hand domain pair	unknown	SSF47473	EF-hand	coord: 45..168 score: 1.37
IPR018247	EF-Hand 1, calcium-binding site	PROSITE	PS00018	EF_HAND_1	coord: 92..104 score: -coord: 127..139 score: -coord: 56..68 scor
None	No IPR available	PANTHER	PTHR10891	EF-HAND CALCIUM-BINDING DOMAIN CONTAINING PROTEIN	coord: 42..169 score: 1.3
None	No IPR available	PANTHER	PTHR10891:SF533	SUBFAMILY NOT NAMED	coord: 42..169 score: 1.3

Synteny

The following gene(s) are orthologous to this gene:


Gene	Orthologue	Organism	Block
Lsi03G004870	Csa7G073720	Cucumber (Chinese Long) v2	culsiB522
Lsi03G004870	CSPI07G07690	Wild cucumber (PI 183967)	cpilsiB536
Lsi03G004870	Cucsa.219910	Cucumber (Gy14) v1	cgylsiB367
Lsi03G004870	CmaCh19G006090	Cucurbita maxima (Rimu)	cmalsiB484
Lsi03G004870	CmoCh11G013580	Cucurbita moschata (Rifu)	cmolsiB103
Lsi03G004870	CmoCh19G006270	Cucurbita moschata (Rifu)	cmolsiB468
Lsi03G004870	Cla016007	Watermelon (97103) v1	lsiwmB268
Lsi03G004870	MELO3C018445	Melon (DHL92) v3.5.1	lsimeB247
Lsi03G004870	ClCG02G005880	Watermelon (Charleston Gray)	lsiwcgB233
Lsi03G004870	MELO3C018445.2	Melon (DHL92) v3.6.1	lsimedB260
Lsi03G004870	Cla97C02G032860	Watermelon (97103) v2	lsiwmbB226
Lsi03G004870	Bhi05G000996	Wax gourd	lsiwgoB321
Lsi03G004870	Cp4.1LG04g08070	Cucurbita pepo (Zucchini)	cpelsiB543
Lsi03G004870	Cp4.1LG15g04910	Cucurbita pepo (Zucchini)	cpelsiB208
Lsi03G004870	CsGy7G007250	Cucumber (Gy14) v2	cgyblsiB488
Lsi03G004870	CsaV3_7G008650	Cucumber (Chinese Long) v3	cuclsiB555

The following gene(s) are paralogous to this gene:

None

The following block(s) are covering this gene:


Gene	Organism	Block
Lsi03G004870	Silver-seed gourd	carlsiB187
Lsi03G004870	Cucumber (Chinese Long) v3	cuclsiB205
Lsi03G004870	Watermelon (97103) v2	lsiwmbB236
Lsi03G004870	Watermelon (97103) v2	lsiwmbB244
Lsi03G004870	Wax gourd	lsiwgoB314
Lsi03G004870	Wax gourd	lsiwgoB323
Lsi03G004870	Bottle gourd (USVL1VR-Ls)	lsilsiB132
Lsi03G004870	Cucumber (Gy14) v1	cgylsiB403
Lsi03G004870	Cucurbita maxima (Rimu)	cmalsiB116
Lsi03G004870	Cucurbita maxima (Rimu)	cmalsiB662
Lsi03G004870	Cucurbita moschata (Rifu)	cmolsiB650
Lsi03G004870	Cucurbita pepo (Zucchini)	cpelsiB171
Lsi03G004870	Cucurbita pepo (Zucchini)	cpelsiB348
Lsi03G004870	Cucurbita pepo (Zucchini)	cpelsiB454
Lsi03G004870	Cucumber (Chinese Long) v2	culsiB189
Lsi03G004870	Melon (DHL92) v3.5.1	lsimeB267
Lsi03G004870	Watermelon (Charleston Gray)	lsiwcgB242
Lsi03G004870	Watermelon (97103) v1	lsiwmB255
Lsi03G004870	Cucumber (Gy14) v2	cgyblsiB103
Lsi03G004870	Cucumber (Gy14) v2	cgyblsiB173
Lsi03G004870	Melon (DHL92) v3.6.1	lsimedB271
Lsi03G004870	Melon (DHL92) v3.6.1	lsimedB280