Cucsa.219910 (gene) Cucumber (Gy14) v1

Overview
NameCucsa.219910
Typegene
OrganismCucumis sativus (Cucumber (Gy14) v1)
DescriptionCalmodulin-related family protein
Locationscaffold01658 : 1503454 .. 1504197 (+)
Genome Viewer
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
TCTTCAAATCCATCTTCCTTCATCTTCAATTTCATTGCCTATCTTCCACTGTTTCAATAAaCAAAAAaCAGAGCAACCTTGTTTTCACTACTCAACCACTGAAATTCCATCTGGGTTCCTCTAAATTTTGAAAACCAATCTTATTTTCATCCAAAAACACAAAAACAGTGCAACCCATCGTTAAAAAAAaTGCCAATTAACCCGAATTTCCTTGAACTACAGTACAACGTCTCAAAGAAAAAGTTCCTCCGTGAACCTTCCCGGCTGTTCTCCTTCAAGGACCGGCAGAGCTCTGGCCTCCGGCGGGCACTGCAGCCGAGCTCGGAGGAGATAAAGCAGATCTTCAACAAGTTTGACACGAACAAAGACGGGAGGATCTCGAAGCATGAATACAGGGGAATTCTGAAGGCGTTGGGGAGAGGGAATTCGATGGAAGAAGTTCAGAAGATTTTTAGGGCGGTGGATTCCGACGGCGATGGGTATATAAATTTGAATGAGTTTATGGAGGTTCACCGGAGCGGCGGGGGAGTTCAGGCAAAAGAGGTGGAATTTGCCTTCAAGACGTTTGATtTGAATGGGGATCGGAAGATTAGTGCAGAGGAAGTGATGAGAGTGTTGAAGGgATTGGGGgAGAAGTGTAGCATTGAGGATTGCCGGCGAATGGTGAGGGCGGTGGATAGCGACGGCGACGGCATGGTGGACATTAATGAGTTTATGACCATGATGACGCGCTCTGCTAAGTGA

mRNA sequence

TCTTCAAATCCATCTTCCTTCATCTTCAATTTCATTGCCTATCTTCCACTGTTTCAATAAACAAAAAACAGAGCAACCTTGTTTTCACTACTCAACCACTGAAATTCCATCTGGGTTCCTCTAAATTTTGAAAACCAATCTTATTTTCATCCAAAAACACAAAAACAGTGCAACCCATCGTTAAAAAAAATGCCAATTAACCCGAATTTCCTTGAACTACAGTACAACGTCTCAAAGAAAAAGTTCCTCCGTGAACCTTCCCGGCTGTTCTCCTTCAAGGACCGGCAGAGCTCTGGCCTCCGGCGGGCACTGCAGCCGAGCTCGGAGGAGATAAAGCAGATCTTCAACAAGTTTGACACGAACAAAGACGGGAGGATCTCGAAGCATGAATACAGGGGAATTCTGAAGGCGTTGGGGAGAGGGAATTCGATGGAAGAAGTTCAGAAGATTTTTAGGGCGGTGGATTCCGACGGCGATGGGTATATAAATTTGAATGAGTTTATGGAGGTTCACCGGAGCGGCGGGGGAGTTCAGGCAAAAGAGGTGGAATTTGCCTTCAAGACGTTTGATTTGAATGGGGATCGGAAGATTAGTGCAGAGGAAGTGATGAGAGTGTTGAAGGGATTGGGGGAGAAGTGTAGCATTGAGGATTGCCGGCGAATGGTGAGGGCGGTGGATAGCGACGGCGACGGCATGGTGGACATTAATGAGTTTATGACCATGATGACGCGCTCTGCTAAGTGA

Coding sequence (CDS)

aTGCCAATTAACCCGAATTTCCTTGAACTACAGTACAACGTCTCAAAGAAAAAGTTCCTCCGTGAACCTTCCCGGCTGTTCTCCTTCAAGGACCGGCAGAGCTCTGGCCTCCGGCGGGCACTGCAGCCGAGCTCGGAGGAGATAAAGCAGATCTTCAACAAGTTTGACACGAACAAAGACGGGAGGATCTCGAAGCATGAATACAGGGGAATTCTGAAGGCGTTGGGGAGAGGGAATTCGATGGAAGAAGTTCAGAAGATTTTTAGGGCGGTGGATTCCGACGGCGATGGGTATATAAATTTGAATGAGTTTATGGAGGTTCACCGGAGCGGCGGGGGAGTTCAGGCAAAAGAGGTGGAATTTGCCTTCAAGACGTTTGATtTGAATGGGGATCGGAAGATTAGTGCAGAGGAAGTGATGAGAGTGTTGAAGGgATTGGGGgAGAAGTGTAGCATTGAGGATTGCCGGCGAATGGTGAGGGCGGTGGATAGCGACGGCGACGGCATGGTGGACATTAATGAGTTTATGACCATGATGACGCGCTCTGCTAAGTGA

Protein sequence

MPINPNFLELQYNVSKKKFLREPSRLFSFKDRQSSGLRRALQPSSEEIKQIFNKFDTNKDGRISKHEYRGILKALGRGNSMEEVQKIFRAVDSDGDGYINLNEFMEVHRSGGGVQAKEVEFAFKTFDLNGDRKISAEEVMRVLKGLGEKCSIEDCRRMVRAVDSDGDGMVDINEFMTMMTRSAK*
Homology
[Close]
BLAST of Cucsa.219910 vs. Swiss-Prot
Match: CML1_ARATH (Calmodulin-like protein 1 OS=Arabidopsis thaliana GN=CML1 PE=2 SV=1)

HSP 1 Score: 207.2 bits (526), Expect = 1.5e-52
Identity = 101/182 (55.49%), Postives = 143/182 (78.57%), Query Frame = 1 

Query: 4   NPNFLELQYNVSKKKFLREPSRLFSFKDRQSSGLRRA-----LQPSSEEIKQIFNKFDTN 63
           N +FLELQY +SK K LR+PSR+FS +DRQSSGL         QPS  E++++F++FD +
Sbjct: 3   NVSFLELQYKLSKNKMLRKPSRMFS-RDRQSSGLSSPGPGGFSQPSVNEMRRVFSRFDLD 62

Query: 64  KDGRISKHEYRGILKALGRGNSMEEVQKIFRAVDSDGDGYINLNEFMEVHRSGGGVQAKE 123
           KDG+IS+ EY+ +L+ALG+  ++E+V KIF+AVD DGDG+I+  EF++ ++  GG+++ +
Sbjct: 63  KDGKISQTEYKVVLRALGQERAIEDVPKIFKAVDLDGDGFIDFREFIDAYKRSGGIRSSD 122

Query: 124 VEFAFKTFDLNGDRKISAEEVMRVLKGLGEKCSIEDCRRMVRAVDSDGDGMVDINEFMTM 181
           +  +F TFDLNGD KISAEEVM VL  LGE+CS+EDC RMVRAVD+DGDG+V++ EF+ M
Sbjct: 123 IRNSFWTFDLNGDGKISAEEVMSVLWKLGERCSLEDCNRMVRAVDADGDGLVNMEEFIKM 182

[Close]
BLAST of Cucsa.219910 vs. Swiss-Prot
Match: CML23_ARATH (Probable calcium-binding protein CML23 OS=Arabidopsis thaliana GN=CML23 PE=2 SV=1)

HSP 1 Score: 124.4 bits (311), Expect = 1.3e-27
Identity = 63/150 (42.00%), Postives = 95/150 (63.33%), Query Frame = 1 

Query: 34  SSGLRRALQPSSEEIKQIFNKFDTNKDGRISKHEYRGILKALGRGNSMEEVQKIFRAVDS 93
           S  + R    S E+IK++F +FD N DG+IS  E + ++ AL    S EE + + +  D 
Sbjct: 2   SKNVSRNCLGSMEDIKKVFQRFDKNNDGKISIDELKDVIGALSPNASQEETKAMMKEFDL 61

Query: 94  DGDGYINLNEFMEVHR----SGGGVQAKEVEFAFKTFDLNGDRKISAEEVMRVLKGLGEK 153
           DG+G+I+L+EF+ + +    S      ++++ AF  +DL+ + +ISA E+  V+K LGEK
Sbjct: 62  DGNGFIDLDEFVALFQISDQSSNNSAIRDLKEAFDLYDLDRNGRISANELHSVMKNLGEK 121

Query: 154 CSIEDCRRMVRAVDSDGDGMVDINEFMTMM 180
           CSI+DC+RM+  VDSDGDG VD  EF  MM
Sbjct: 122 CSIQDCQRMINKVDSDGDGCVDFEEFKKMM 151

[Close]
BLAST of Cucsa.219910 vs. Swiss-Prot
Match: CML27_ARATH (Probable calcium-binding protein CML27 OS=Arabidopsis thaliana GN=CML27 PE=1 SV=1)

HSP 1 Score: 124.0 bits (310), Expect = 1.7e-27
Identity = 63/138 (45.65%), Postives = 90/138 (65.22%), Query Frame = 1 

Query: 46  EEIKQIFNKFDTNKDGRISKHEYRGILKALGRGNSMEEVQKIFRAVDSDGDGYINLNEFM 105
           EE+K++F++FD+N DG+IS  E  G+ KA+G   +  E+ ++   VD+D DGYINL+EF 
Sbjct: 22  EELKKVFDQFDSNGDGKISVLELGGVFKAMGTSYTETELNRVLEEVDTDRDGYINLDEFS 81

Query: 106 EVHRSGGGVQAKEVEFAFKTFDLNGDRKISAEEVMRVLKGLGEKCSIEDCRRMVRAVDSD 165
            + RS     A E+  AF  +D + +  ISA E+ +VL  LG  CS+EDC RM+  VD+D
Sbjct: 82  TLCRSSS--SAAEIRDAFDLYDQDKNGLISASELHQVLNRLGMSCSVEDCTRMIGPVDAD 141

Query: 166 GDGMVDINEFMTMMTRSA 184
           GDG V+  EF  MMT S+
Sbjct: 142 GDGNVNFEEFQKMMTSSS 157

[Close]
BLAST of Cucsa.219910 vs. Swiss-Prot
Match: CML10_ORYSJ (Probable calcium-binding protein CML10 OS=Oryza sativa subsp. japonica GN=CML10 PE=2 SV=1)

HSP 1 Score: 123.6 bits (309), Expect = 2.2e-27
Identity = 62/152 (40.79%), Postives = 96/152 (63.16%), Query Frame = 1 

Query: 31  DRQSSGLRRALQPSSEEIKQIFNKFDTNKDGRISKHEYRGILKALGRGNSMEEVQKIFRA 90
           D  S G   A  P  EE++++F KFD N DGRIS+ E   + ++LG   + +E+ ++   
Sbjct: 25  DPASGGGSPAPTPE-EEMERVFRKFDANGDGRISRSELGALFESLGHAATDDELARMMAE 84

Query: 91  VDSDGDGYINLNEFMEVHRSGGGVQA---KEVEFAFKTFDLNGDRKISAEEVMRVLKGLG 150
            D+DGDG+I+L+EF  ++ +  G  A   +++  AF+ FD +G+  ISA E+ RVL GLG
Sbjct: 85  ADADGDGFISLDEFAALNATASGDAAAVEEDLRHAFRVFDADGNGTISAAELARVLHGLG 144

Query: 151 EKCSIEDCRRMVRAVDSDGDGMVDINEFMTMM 180
           EK +++ CRRM+  VD +GDG++   EF  MM
Sbjct: 145 EKATVQQCRRMIEGVDQNGDGLISFEEFKVMM 175

[Close]
BLAST of Cucsa.219910 vs. Swiss-Prot
Match: CML25_ARATH (Probable calcium-binding protein CML25 OS=Arabidopsis thaliana GN=CML25 PE=2 SV=1)

HSP 1 Score: 123.2 bits (308), Expect = 2.9e-27
Identity = 59/140 (42.14%), Postives = 88/140 (62.86%), Query Frame = 1 

Query: 47  EIKQIFNKFDTNKDGRISKHEYRGILKALGRGNSMEEVQKIFRAVDSDGDGYINLNEFME 106
           E++ +F KFD N DG+IS  E   I+ +LG     EE++K    +D  GDGYIN  EF+E
Sbjct: 37  ELEAVFKKFDVNGDGKISSKELGAIMTSLGHEVPEEELEKAITEIDRKGDGYINFEEFVE 96

Query: 107 VHRSGGGVQ--AKEVEFAFKTFDLNGDRKISAEEVMRVLKGLGEKCSIEDCRRMVRAVDS 166
           ++  G       + ++ AF  +D++G+  ISAEE+  VL+ LG++CSI +CR+M+  VD 
Sbjct: 97  LNTKGMDQNDVLENLKDAFSVYDIDGNGSISAEELHEVLRSLGDECSIAECRKMIGGVDK 156

Query: 167 DGDGMVDINEFMTMMTRSAK 185
           DGDG +D  EF  MMT  ++
Sbjct: 157 DGDGTIDFEEFKIMMTMGSR 176

[Close]
BLAST of Cucsa.219910 vs. TrEMBL
Match: A0A0A0K804_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_7G073720 PE=4 SV=1)

HSP 1 Score: 367.5 bits (942), Expect = 9.8e-99
Identity = 184/184 (100.00%), Postives = 184/184 (100.00%), Query Frame = 1 

Query: 1   MPINPNFLELQYNVSKKKFLREPSRLFSFKDRQSSGLRRALQPSSEEIKQIFNKFDTNKD 60
           MPINPNFLELQYNVSKKKFLREPSRLFSFKDRQSSGLRRALQPSSEEIKQIFNKFDTNKD
Sbjct: 1   MPINPNFLELQYNVSKKKFLREPSRLFSFKDRQSSGLRRALQPSSEEIKQIFNKFDTNKD 60

Query: 61  GRISKHEYRGILKALGRGNSMEEVQKIFRAVDSDGDGYINLNEFMEVHRSGGGVQAKEVE 120
           GRISKHEYRGILKALGRGNSMEEVQKIFRAVDSDGDGYINLNEFMEVHRSGGGVQAKEVE
Sbjct: 61  GRISKHEYRGILKALGRGNSMEEVQKIFRAVDSDGDGYINLNEFMEVHRSGGGVQAKEVE 120

Query: 121 FAFKTFDLNGDRKISAEEVMRVLKGLGEKCSIEDCRRMVRAVDSDGDGMVDINEFMTMMT 180
           FAFKTFDLNGDRKISAEEVMRVLKGLGEKCSIEDCRRMVRAVDSDGDGMVDINEFMTMMT
Sbjct: 121 FAFKTFDLNGDRKISAEEVMRVLKGLGEKCSIEDCRRMVRAVDSDGDGMVDINEFMTMMT 180

Query: 181 RSAK 185
           RSAK
Sbjct: 181 RSAK 184

[Close]
BLAST of Cucsa.219910 vs. TrEMBL
Match: M5VNS5_PRUPE (Uncharacterized protein OS=Prunus persica GN=PRUPE_ppa011990mg PE=4 SV=1)

HSP 1 Score: 253.1 bits (645), Expect = 2.7e-64
Identity = 117/179 (65.36%), Postives = 152/179 (84.92%), Query Frame = 1 

Query: 6   NFLELQYNVSKKKFLREPSRLFSFKDRQSSGLRRALQPSSEEIKQIFNKFDTNKDGRISK 65
           NFL+ QYN+SK+KFLR+PS +FSF+DRQ+SG+  + +P+  E+K++F+KFD+NKDG+IS+
Sbjct: 5   NFLDFQYNLSKRKFLRKPSHVFSFRDRQNSGVSPSFEPNPNEMKRVFDKFDSNKDGKISQ 64

Query: 66  HEYRGILKALGRGNSMEEVQKIFRAVDSDGDGYINLNEFMEVHRSGGGVQAKEVEFAFKT 125
            EY+ IL+ALG+GN + EV KIFR  D DGDG+IN  EFMEVH+ GGG++  +++ AF+T
Sbjct: 65  PEYKAILRALGKGNMVGEVPKIFRIADLDGDGFINFKEFMEVHKKGGGIRTIDIQNAFRT 124

Query: 126 FDLNGDRKISAEEVMRVLKGLGEKCSIEDCRRMVRAVDSDGDGMVDINEFMTMMTRSAK 185
           FDLNGD KISAEEVM VL  LGE CS+EDC+RMVRAVD+DGDG+VDI+EFMTMMTRS K
Sbjct: 125 FDLNGDGKISAEEVMEVLGRLGESCSLEDCQRMVRAVDTDGDGVVDIDEFMTMMTRSMK 183

[Close]
BLAST of Cucsa.219910 vs. TrEMBL
Match: B9HS04_POPTR (Calmodulin-related family protein OS=Populus trichocarpa GN=POPTR_0009s10530g PE=4 SV=1)

HSP 1 Score: 239.2 bits (609), Expect = 4.0e-60
Identity = 111/179 (62.01%), Postives = 147/179 (82.12%), Query Frame = 1 

Query: 4   NPNFLELQYNVSKKKFLREPSRLFSFKDRQSSGLRRALQPSSEEIKQIFNKFDTNKDGRI 63
           N +FLE QY +S+ KFLR+PSRLFS +DRQSSG+    QP+ +E++Q+F+KFD+NKDG+I
Sbjct: 3   NLSFLEFQYKLSRNKFLRKPSRLFSSRDRQSSGMLSTFQPNMKEMRQVFDKFDSNKDGKI 62

Query: 64  SKHEYRGILKALGRGNSMEEVQKIFRAVDSDGDGYINLNEFMEVHRSGGGVQAKEVEFAF 123
           S+ EY+  L+ALG+GN + EV KIF+ VD DGDG+I+  EF+E  + GGG++  +++ AF
Sbjct: 63  SQQEYKDTLRALGQGNMLGEVPKIFQVVDLDGDGFIDFKEFVEAQKKGGGIRTTDIQTAF 122

Query: 124 KTFDLNGDRKISAEEVMRVLKGLGEKCSIEDCRRMVRAVDSDGDGMVDINEFMTMMTRS 183
           +TFD NGD KISAEEVM VL+ LGE+CS+EDCRRMV AVD DGDGMV+++EFMTMMTRS
Sbjct: 123 QTFDSNGDGKISAEEVMEVLRRLGERCSLEDCRRMVNAVDIDGDGMVNMDEFMTMMTRS 181

[Close]
BLAST of Cucsa.219910 vs. TrEMBL
Match: A0A0B2RCG8_GLYSO (Calmodulin-like protein 1 OS=Glycine soja GN=glysoja_020368 PE=4 SV=1)

HSP 1 Score: 233.4 bits (594), Expect = 2.2e-58
Identity = 112/180 (62.22%), Postives = 147/180 (81.67%), Query Frame = 1 

Query: 6   NFLELQYNVSKKKFLREPSRLFSFKDRQSSGLRRA-LQPSSEEIKQIFNKFDTNKDGRIS 65
           +FL+ QYN+S++K+LR+PSRLFS +DRQ+SGL+   LQP+++E+KQ+F+KFD+NKDG+IS
Sbjct: 97  SFLDFQYNLSRRKYLRKPSRLFS-RDRQNSGLKGVFLQPNADEMKQVFDKFDSNKDGKIS 156

Query: 66  KHEYRGILKALGRGNSMEEVQKIFRAVDSDGDGYINLNEFMEVHRSGGGVQAKEVEFAFK 125
           + EY+  +KALG G+S+ EV  IFR VD DGDG+IN  EFME    GGGV+  ++  AF+
Sbjct: 157 QQEYKATMKALGMGDSVHEVPNIFRVVDLDGDGFINFKEFMEAQNKGGGVRTMDIHSAFR 216

Query: 126 TFDLNGDRKISAEEVMRVLKGLGEKCSIEDCRRMVRAVDSDGDGMVDINEFMTMMTRSAK 185
           TFD NGD +ISAEEV   L  LGE+CSIEDCRRMVRAVD+DGDGMVD++EF TMMT+S +
Sbjct: 217 TFDRNGDGRISAEEVKETLGRLGERCSIEDCRRMVRAVDTDGDGMVDMDEFTTMMTQSLR 275

[Close]
BLAST of Cucsa.219910 vs. TrEMBL
Match: C6TAX4_SOYBN (Uncharacterized protein OS=Glycine max GN=GLYMA_07G212000 PE=2 SV=1)

HSP 1 Score: 233.4 bits (594), Expect = 2.2e-58
Identity = 112/180 (62.22%), Postives = 147/180 (81.67%), Query Frame = 1 

Query: 6   NFLELQYNVSKKKFLREPSRLFSFKDRQSSGLRRA-LQPSSEEIKQIFNKFDTNKDGRIS 65
           +FL+ QYN+S++K+LR+PSRLFS +DRQ+SGL+   LQP+++E+KQ+F+KFD+NKDG+IS
Sbjct: 5   SFLDFQYNLSRRKYLRKPSRLFS-RDRQNSGLKGVFLQPNADEMKQVFDKFDSNKDGKIS 64

Query: 66  KHEYRGILKALGRGNSMEEVQKIFRAVDSDGDGYINLNEFMEVHRSGGGVQAKEVEFAFK 125
           + EY+  +KALG G+S+ EV  IFR VD DGDG+IN  EFME    GGGV+  ++  AF+
Sbjct: 65  QQEYKATMKALGMGDSVHEVPNIFRVVDLDGDGFINFKEFMEAQNKGGGVRTMDIHSAFR 124

Query: 126 TFDLNGDRKISAEEVMRVLKGLGEKCSIEDCRRMVRAVDSDGDGMVDINEFMTMMTRSAK 185
           TFD NGD +ISAEEV   L  LGE+CSIEDCRRMVRAVD+DGDGMVD++EF TMMT+S +
Sbjct: 125 TFDRNGDGRISAEEVKETLGRLGERCSIEDCRRMVRAVDTDGDGMVDMDEFTTMMTQSLR 183

[Close]
BLAST of Cucsa.219910 vs. TAIR10
Match: AT2G15680.1 (AT2G15680.1 Calcium-binding EF-hand family protein)

HSP 1 Score: 207.2 bits (526), Expect = 8.6e-54
Identity = 101/182 (55.49%), Postives = 143/182 (78.57%), Query Frame = 1 

Query: 4   NPNFLELQYNVSKKKFLREPSRLFSFKDRQSSGLRRA-----LQPSSEEIKQIFNKFDTN 63
           N +FLELQY +SK K LR+PSR+FS +DRQSSGL         QPS  E++++F++FD +
Sbjct: 3   NVSFLELQYKLSKNKMLRKPSRMFS-RDRQSSGLSSPGPGGFSQPSVNEMRRVFSRFDLD 62

Query: 64  KDGRISKHEYRGILKALGRGNSMEEVQKIFRAVDSDGDGYINLNEFMEVHRSGGGVQAKE 123
           KDG+IS+ EY+ +L+ALG+  ++E+V KIF+AVD DGDG+I+  EF++ ++  GG+++ +
Sbjct: 63  KDGKISQTEYKVVLRALGQERAIEDVPKIFKAVDLDGDGFIDFREFIDAYKRSGGIRSSD 122

Query: 124 VEFAFKTFDLNGDRKISAEEVMRVLKGLGEKCSIEDCRRMVRAVDSDGDGMVDINEFMTM 181
           +  +F TFDLNGD KISAEEVM VL  LGE+CS+EDC RMVRAVD+DGDG+V++ EF+ M
Sbjct: 123 IRNSFWTFDLNGDGKISAEEVMSVLWKLGERCSLEDCNRMVRAVDADGDGLVNMEEFIKM 182

[Close]
BLAST of Cucsa.219910 vs. TAIR10
Match: AT1G66400.1 (AT1G66400.1 calmodulin like 23)

HSP 1 Score: 124.4 bits (311), Expect = 7.3e-29
Identity = 63/150 (42.00%), Postives = 95/150 (63.33%), Query Frame = 1 

Query: 34  SSGLRRALQPSSEEIKQIFNKFDTNKDGRISKHEYRGILKALGRGNSMEEVQKIFRAVDS 93
           S  + R    S E+IK++F +FD N DG+IS  E + ++ AL    S EE + + +  D 
Sbjct: 2   SKNVSRNCLGSMEDIKKVFQRFDKNNDGKISIDELKDVIGALSPNASQEETKAMMKEFDL 61

Query: 94  DGDGYINLNEFMEVHR----SGGGVQAKEVEFAFKTFDLNGDRKISAEEVMRVLKGLGEK 153
           DG+G+I+L+EF+ + +    S      ++++ AF  +DL+ + +ISA E+  V+K LGEK
Sbjct: 62  DGNGFIDLDEFVALFQISDQSSNNSAIRDLKEAFDLYDLDRNGRISANELHSVMKNLGEK 121

Query: 154 CSIEDCRRMVRAVDSDGDGMVDINEFMTMM 180
           CSI+DC+RM+  VDSDGDG VD  EF  MM
Sbjct: 122 CSIQDCQRMINKVDSDGDGCVDFEEFKKMM 151

[Close]
BLAST of Cucsa.219910 vs. TAIR10
Match: AT1G18210.1 (AT1G18210.1 Calcium-binding EF-hand family protein)

HSP 1 Score: 124.0 bits (310), Expect = 9.5e-29
Identity = 63/138 (45.65%), Postives = 90/138 (65.22%), Query Frame = 1 

Query: 46  EEIKQIFNKFDTNKDGRISKHEYRGILKALGRGNSMEEVQKIFRAVDSDGDGYINLNEFM 105
           EE+K++F++FD+N DG+IS  E  G+ KA+G   +  E+ ++   VD+D DGYINL+EF 
Sbjct: 22  EELKKVFDQFDSNGDGKISVLELGGVFKAMGTSYTETELNRVLEEVDTDRDGYINLDEFS 81

Query: 106 EVHRSGGGVQAKEVEFAFKTFDLNGDRKISAEEVMRVLKGLGEKCSIEDCRRMVRAVDSD 165
            + RS     A E+  AF  +D + +  ISA E+ +VL  LG  CS+EDC RM+  VD+D
Sbjct: 82  TLCRSSS--SAAEIRDAFDLYDQDKNGLISASELHQVLNRLGMSCSVEDCTRMIGPVDAD 141

Query: 166 GDGMVDINEFMTMMTRSA 184
           GDG V+  EF  MMT S+
Sbjct: 142 GDGNVNFEEFQKMMTSSS 157

[Close]
BLAST of Cucsa.219910 vs. TAIR10
Match: AT1G24620.1 (AT1G24620.1 EF hand calcium-binding protein family)

HSP 1 Score: 123.2 bits (308), Expect = 1.6e-28
Identity = 59/140 (42.14%), Postives = 88/140 (62.86%), Query Frame = 1 

Query: 47  EIKQIFNKFDTNKDGRISKHEYRGILKALGRGNSMEEVQKIFRAVDSDGDGYINLNEFME 106
           E++ +F KFD N DG+IS  E   I+ +LG     EE++K    +D  GDGYIN  EF+E
Sbjct: 37  ELEAVFKKFDVNGDGKISSKELGAIMTSLGHEVPEEELEKAITEIDRKGDGYINFEEFVE 96

Query: 107 VHRSGGGVQ--AKEVEFAFKTFDLNGDRKISAEEVMRVLKGLGEKCSIEDCRRMVRAVDS 166
           ++  G       + ++ AF  +D++G+  ISAEE+  VL+ LG++CSI +CR+M+  VD 
Sbjct: 97  LNTKGMDQNDVLENLKDAFSVYDIDGNGSISAEELHEVLRSLGDECSIAECRKMIGGVDK 156

Query: 167 DGDGMVDINEFMTMMTRSAK 185
           DGDG +D  EF  MMT  ++
Sbjct: 157 DGDGTIDFEEFKIMMTMGSR 176

[Close]
BLAST of Cucsa.219910 vs. TAIR10
Match: AT5G37770.1 (AT5G37770.1 EF hand calcium-binding protein family)

HSP 1 Score: 122.9 bits (307), Expect = 2.1e-28
Identity = 62/152 (40.79%), Postives = 102/152 (67.11%), Query Frame = 1 

Query: 35  SGLRRALQPSSEEIKQIFNKFDTNKDGRISKHEYRGILKALGRGNSMEEVQKIFRAVDSD 94
           +G+ R+   S ++IK++F +FD N DG+IS  E + +++AL    S EE   + +  D D
Sbjct: 5   NGVVRSCLGSMDDIKKVFQRFDKNGDGKISVDELKEVIRALSPTASPEETVTMMKQFDLD 64

Query: 95  GDGYINLNEFMEVHR---SGGGVQAKEV---EFAFKTFDLNGDRKISAEEVMRVLKGLGE 154
           G+G+I+L+EF+ + +    GGG    +V   + AF+ +DL+G+ +ISA+E+  V+K LGE
Sbjct: 65  GNGFIDLDEFVALFQIGIGGGGNNRNDVSDLKEAFELYDLDGNGRISAKELHSVMKNLGE 124

Query: 155 KCSIEDCRRMVRAVDSDGDGMVDINEFMTMMT 181
           KCS++DC++M+  VD DGDG V+ +EF  MM+
Sbjct: 125 KCSVQDCKKMISKVDIDGDGCVNFDEFKKMMS 156

[Close]
BLAST of Cucsa.219910 vs. NCBI nr
Match: gi|778725125|ref|XP_004137114.2| (PREDICTED: calmodulin-like protein 1 [Cucumis sativus])

HSP 1 Score: 367.5 bits (942), Expect = 1.4e-98
Identity = 184/184 (100.00%), Postives = 184/184 (100.00%), Query Frame = 1 

Query: 1   MPINPNFLELQYNVSKKKFLREPSRLFSFKDRQSSGLRRALQPSSEEIKQIFNKFDTNKD 60
           MPINPNFLELQYNVSKKKFLREPSRLFSFKDRQSSGLRRALQPSSEEIKQIFNKFDTNKD
Sbjct: 1   MPINPNFLELQYNVSKKKFLREPSRLFSFKDRQSSGLRRALQPSSEEIKQIFNKFDTNKD 60

Query: 61  GRISKHEYRGILKALGRGNSMEEVQKIFRAVDSDGDGYINLNEFMEVHRSGGGVQAKEVE 120
           GRISKHEYRGILKALGRGNSMEEVQKIFRAVDSDGDGYINLNEFMEVHRSGGGVQAKEVE
Sbjct: 61  GRISKHEYRGILKALGRGNSMEEVQKIFRAVDSDGDGYINLNEFMEVHRSGGGVQAKEVE 120

Query: 121 FAFKTFDLNGDRKISAEEVMRVLKGLGEKCSIEDCRRMVRAVDSDGDGMVDINEFMTMMT 180
           FAFKTFDLNGDRKISAEEVMRVLKGLGEKCSIEDCRRMVRAVDSDGDGMVDINEFMTMMT
Sbjct: 121 FAFKTFDLNGDRKISAEEVMRVLKGLGEKCSIEDCRRMVRAVDSDGDGMVDINEFMTMMT 180

Query: 181 RSAK 185
           RSAK
Sbjct: 181 RSAK 184

[Close]
BLAST of Cucsa.219910 vs. NCBI nr
Match: gi|659109907|ref|XP_008454944.1| (PREDICTED: calmodulin-like protein 1 [Cucumis melo])

HSP 1 Score: 362.1 bits (928), Expect = 5.9e-97
Identity = 181/184 (98.37%), Postives = 183/184 (99.46%), Query Frame = 1 

Query: 1   MPINPNFLELQYNVSKKKFLREPSRLFSFKDRQSSGLRRALQPSSEEIKQIFNKFDTNKD 60
           MPINPNFL+LQYNVSKKKFLREPSRLFSFKDRQSSGLRRALQPS EEIKQIFNKFDTNKD
Sbjct: 1   MPINPNFLDLQYNVSKKKFLREPSRLFSFKDRQSSGLRRALQPSLEEIKQIFNKFDTNKD 60

Query: 61  GRISKHEYRGILKALGRGNSMEEVQKIFRAVDSDGDGYINLNEFMEVHRSGGGVQAKEVE 120
           GRISKHEYRGILKALGRG+SMEEVQKIFRAVDSDGDGYINLNEFMEVHRSGGGVQAKEVE
Sbjct: 61  GRISKHEYRGILKALGRGSSMEEVQKIFRAVDSDGDGYINLNEFMEVHRSGGGVQAKEVE 120

Query: 121 FAFKTFDLNGDRKISAEEVMRVLKGLGEKCSIEDCRRMVRAVDSDGDGMVDINEFMTMMT 180
           FAFKTFDLNGDRKISAEEVMRVLKGLGEKCSIEDCRRMVRAVDSDGDGMVDINEFMTMMT
Sbjct: 121 FAFKTFDLNGDRKISAEEVMRVLKGLGEKCSIEDCRRMVRAVDSDGDGMVDINEFMTMMT 180

Query: 181 RSAK 185
           RSAK
Sbjct: 181 RSAK 184

[Close]
BLAST of Cucsa.219910 vs. NCBI nr
Match: gi|595798018|ref|XP_007201342.1| (hypothetical protein PRUPE_ppa011990mg [Prunus persica])

HSP 1 Score: 253.1 bits (645), Expect = 3.9e-64
Identity = 117/179 (65.36%), Postives = 152/179 (84.92%), Query Frame = 1 

Query: 6   NFLELQYNVSKKKFLREPSRLFSFKDRQSSGLRRALQPSSEEIKQIFNKFDTNKDGRISK 65
           NFL+ QYN+SK+KFLR+PS +FSF+DRQ+SG+  + +P+  E+K++F+KFD+NKDG+IS+
Sbjct: 5   NFLDFQYNLSKRKFLRKPSHVFSFRDRQNSGVSPSFEPNPNEMKRVFDKFDSNKDGKISQ 64

Query: 66  HEYRGILKALGRGNSMEEVQKIFRAVDSDGDGYINLNEFMEVHRSGGGVQAKEVEFAFKT 125
            EY+ IL+ALG+GN + EV KIFR  D DGDG+IN  EFMEVH+ GGG++  +++ AF+T
Sbjct: 65  PEYKAILRALGKGNMVGEVPKIFRIADLDGDGFINFKEFMEVHKKGGGIRTIDIQNAFRT 124

Query: 126 FDLNGDRKISAEEVMRVLKGLGEKCSIEDCRRMVRAVDSDGDGMVDINEFMTMMTRSAK 185
           FDLNGD KISAEEVM VL  LGE CS+EDC+RMVRAVD+DGDG+VDI+EFMTMMTRS K
Sbjct: 125 FDLNGDGKISAEEVMEVLGRLGESCSLEDCQRMVRAVDTDGDGVVDIDEFMTMMTRSMK 183

[Close]
BLAST of Cucsa.219910 vs. NCBI nr
Match: gi|645261897|ref|XP_008236514.1| (PREDICTED: calmodulin-like protein 1 [Prunus mume])

HSP 1 Score: 252.3 bits (643), Expect = 6.6e-64
Identity = 116/179 (64.80%), Postives = 151/179 (84.36%), Query Frame = 1 

Query: 6   NFLELQYNVSKKKFLREPSRLFSFKDRQSSGLRRALQPSSEEIKQIFNKFDTNKDGRISK 65
           NFL+ Q+N+SK+KFLR+PS + SF+DRQ+SGL  + +P+  E+K +F KFD+NKDG+IS+
Sbjct: 5   NFLDFQFNLSKRKFLRKPSHVCSFRDRQNSGLSPSFEPNPNEMKIVFEKFDSNKDGKISQ 64

Query: 66  HEYRGILKALGRGNSMEEVQKIFRAVDSDGDGYINLNEFMEVHRSGGGVQAKEVEFAFKT 125
            EY+ IL+ALG+GN++ EV KIFR  D DGDG+IN  EFMEVH+ GGG++  +++ AF+T
Sbjct: 65  EEYKAILRALGKGNTVREVPKIFRIADLDGDGFINFKEFMEVHKKGGGIRTMDIQNAFRT 124

Query: 126 FDLNGDRKISAEEVMRVLKGLGEKCSIEDCRRMVRAVDSDGDGMVDINEFMTMMTRSAK 185
           FDLNGD KISAEEVM VL+ LGE CS+EDC+RMVRAVD+DGDG+VDI+EFMTMMTRS K
Sbjct: 125 FDLNGDGKISAEEVMEVLRRLGESCSLEDCQRMVRAVDTDGDGVVDIDEFMTMMTRSMK 183

[Close]
BLAST of Cucsa.219910 vs. NCBI nr
Match: gi|764562626|ref|XP_011461491.1| (PREDICTED: calmodulin-like protein 1 [Fragaria vesca subsp. vesca])

HSP 1 Score: 250.8 bits (639), Expect = 1.9e-63
Identity = 117/181 (64.64%), Postives = 152/181 (83.98%), Query Frame = 1 

Query: 4   NPNFLELQYNVSKKKFLREPSRLFSFKDRQSSGLRRALQPSSEEIKQIFNKFDTNKDGRI 63
           N +FL+ QYN+SK+KFLR P+RLFS +DRQ+SGL    +P+  E+K++F KFDTNKDG+I
Sbjct: 3   NMSFLDFQYNLSKRKFLRTPTRLFSSRDRQNSGLTPGFEPNLNEMKRVFEKFDTNKDGKI 62

Query: 64  SKHEYRGILKALGRGNSMEEVQKIFRAVDSDGDGYINLNEFMEVHRSGGGVQAKEVEFAF 123
           S+ +YR IL+ALG+G+ + EV KIF+ VD DGDG+IN  EFMEVH+  GGV+  +++ AF
Sbjct: 63  SQQDYRAILRALGKGSMVGEVPKIFKLVDLDGDGFINFKEFMEVHKKSGGVKTIDIQNAF 122

Query: 124 KTFDLNGDRKISAEEVMRVLKGLGEKCSIEDCRRMVRAVDSDGDGMVDINEFMTMMTRSA 183
           +TFDLNGD KISAEEVM VLK LGE+CS+E+C+RMVRAVD+DGDGMVD++EFMTMMTRS 
Sbjct: 123 QTFDLNGDGKISAEEVMEVLKRLGERCSLEECQRMVRAVDTDGDGMVDLDEFMTMMTRSM 182

Query: 184 K 185
           +
Sbjct: 183 R 183

The following BLAST results are available for this feature:
BLAST of Cucsa.219910 vs. Swiss-Prot
Match NameE-valueIdentityDescription
CML1_ARATH1.5e-5255.49Calmodulin-like protein 1 OS=Arabidopsis thaliana GN=CML1 PE=2 SV=1[more]
CML23_ARATH1.3e-2742.00Probable calcium-binding protein CML23 OS=Arabidopsis thaliana GN=CML23 PE=2 SV=... [more]
CML27_ARATH1.7e-2745.65Probable calcium-binding protein CML27 OS=Arabidopsis thaliana GN=CML27 PE=1 SV=... [more]
CML10_ORYSJ2.2e-2740.79Probable calcium-binding protein CML10 OS=Oryza sativa subsp. japonica GN=CML10 ... [more]
CML25_ARATH2.9e-2742.14Probable calcium-binding protein CML25 OS=Arabidopsis thaliana GN=CML25 PE=2 SV=... [more]
BLAST of Cucsa.219910 vs. TrEMBL
Match NameE-valueIdentityDescription
A0A0A0K804_CUCSA9.8e-99100.00Uncharacterized protein OS=Cucumis sativus GN=Csa_7G073720 PE=4 SV=1[more]
M5VNS5_PRUPE2.7e-6465.36Uncharacterized protein OS=Prunus persica GN=PRUPE_ppa011990mg PE=4 SV=1[more]
B9HS04_POPTR4.0e-6062.01Calmodulin-related family protein OS=Populus trichocarpa GN=POPTR_0009s10530g PE... [more]
A0A0B2RCG8_GLYSO2.2e-5862.22Calmodulin-like protein 1 OS=Glycine soja GN=glysoja_020368 PE=4 SV=1[more]
C6TAX4_SOYBN2.2e-5862.22Uncharacterized protein OS=Glycine max GN=GLYMA_07G212000 PE=2 SV=1[more]
BLAST of Cucsa.219910 vs. TAIR10
Match NameE-valueIdentityDescription
AT2G15680.18.6e-5455.49 Calcium-binding EF-hand family protein[more]
AT1G66400.17.3e-2942.00 calmodulin like 23[more]
AT1G18210.19.5e-2945.65 Calcium-binding EF-hand family protein[more]
AT1G24620.11.6e-2842.14 EF hand calcium-binding protein family[more]
AT5G37770.12.1e-2840.79 EF hand calcium-binding protein family[more]
BLAST of Cucsa.219910 vs. NCBI nr
Match NameE-valueIdentityDescription
gi|778725125|ref|XP_004137114.2|1.4e-98100.00PREDICTED: calmodulin-like protein 1 [Cucumis sativus][more]
gi|659109907|ref|XP_008454944.1|5.9e-9798.37PREDICTED: calmodulin-like protein 1 [Cucumis melo][more]
gi|595798018|ref|XP_007201342.1|3.9e-6465.36hypothetical protein PRUPE_ppa011990mg [Prunus persica][more]
gi|645261897|ref|XP_008236514.1|6.6e-6464.80PREDICTED: calmodulin-like protein 1 [Prunus mume][more]
gi|764562626|ref|XP_011461491.1|1.9e-6364.64PREDICTED: calmodulin-like protein 1 [Fragaria vesca subsp. vesca][more]
Annotated Terms
The following terms have been associated with this gene:
Vocabulary: INTERPRO
TermDefinition
IPR002048EF_hand_dom
IPR011992EF-hand-dom_pair
IPR018247EF_Hand_1_Ca_BS
Vocabulary: Molecular Function
TermDefinition
GO:0005509calcium ion binding
GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0008150 biological_process
cellular_component GO:0005739 mitochondrion
cellular_component GO:0005575 cellular_component
molecular_function GO:0005509 calcium ion binding
Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Cucsa.219910.1Cucsa.219910.1mRNA


InterPro
Analysis Name: InterPro Annotations of cucumber (Gy14)
Date Performed: 2017-01-17
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR002048EF-hand domainPFAMPF13499EF-hand_7coord: 119..179
score: 5.2E-11coord: 46..106
score: 4.3
IPR002048EF-hand domainSMARTSM00054efh_1coord: 83..111
score: 8.3E-5coord: 118..146
score: 3.6E-4coord: 154..182
score: 0.0086coord: 47..75
score: 6.
IPR002048EF-hand domainPROFILEPS50222EF_HAND_2coord: 79..114
score: 14.43coord: 150..184
score: 11.417coord: 43..78
score: 15.936coord: 117..149
score: 12
IPR011992EF-hand domain pairGENE3DG3DSA:1.10.238.10coord: 130..181
score: 1.9E-19coord: 45..129
score: 1.1
IPR011992EF-hand domain pairunknownSSF47473EF-handcoord: 46..180
score: 2.58
IPR018247EF-Hand 1, calcium-binding sitePROSITEPS00018EF_HAND_1coord: 127..139
score: -coord: 56..68
score: -coord: 92..104
score: -coord: 163..175
scor
NoneNo IPR availablePANTHERPTHR10891EF-HAND CALCIUM-BINDING DOMAIN CONTAINING PROTEINcoord: 42..184
score: 3.8
NoneNo IPR availablePANTHERPTHR10891:SF533SUBFAMILY NOT NAMEDcoord: 42..184
score: 3.8

Synteny
The following gene(s) are orthologous to this gene:
GeneOrthologueOrganismBlock
Cucsa.219910Cla016007Watermelon (97103) v1cgywmB427
Cucsa.219910Csa7G073720Cucumber (Chinese Long) v2cgycuB322
Cucsa.219910MELO3C018445Melon (DHL92) v3.5.1cgymeB379
Cucsa.219910ClCG02G005880Watermelon (Charleston Gray)cgywcgB396
Cucsa.219910CSPI07G07690Wild cucumber (PI 183967)cgycpiB336
Cucsa.219910CmoCh11G013580Cucurbita moschata (Rifu)cgycmoB0624
Cucsa.219910Lsi03G004870Bottle gourd (USVL1VR-Ls)cgylsiB367
Cucsa.219910Cp4.1LG04g08070Cucurbita pepo (Zucchini)cgycpeB0602
Cucsa.219910Cp4.1LG15g04910Cucurbita pepo (Zucchini)cgycpeB0594
Cucsa.219910MELO3C018445.2Melon (DHL92) v3.6.1cgymedB377
Cucsa.219910CsaV3_7G008650Cucumber (Chinese Long) v3cgycucB342
Cucsa.219910Cla97C02G032860Watermelon (97103) v2cgywmbB395
Cucsa.219910Bhi05G000996Wax gourdcgywgoB446
Cucsa.219910Carg20367Silver-seed gourdcarcgyB0618
The following gene(s) are paralogous to this gene:

None

The following block(s) are covering this gene:
GeneOrganismBlock
Cucsa.219910Cucumber (Chinese Long) v3cgycucB338
Cucsa.219910Cucumber (Chinese Long) v3cgycucB340
Cucsa.219910Watermelon (97103) v2cgywmbB396
Cucsa.219910Watermelon (97103) v2cgywmbB392
Cucsa.219910Watermelon (97103) v2cgywmbB398
Cucsa.219910Wax gourdcgywgoB444
Cucsa.219910Wax gourdcgywgoB452
Cucsa.219910Cucumber (Gy14) v1cgycgyB117
Cucsa.219910Cucurbita maxima (Rimu)cgycmaB0620
Cucsa.219910Cucurbita maxima (Rimu)cgycmaB0624
Cucsa.219910Cucurbita maxima (Rimu)cgycmaB0629
Cucsa.219910Cucurbita maxima (Rimu)cgycmaB0630
Cucsa.219910Cucurbita moschata (Rifu)cgycmoB0626
Cucsa.219910Cucurbita moschata (Rifu)cgycmoB0627
Cucsa.219910Cucurbita moschata (Rifu)cgycmoB0632
Cucsa.219910Cucurbita moschata (Rifu)cgycmoB0633
Cucsa.219910Wild cucumber (PI 183967)cgycpiB331
Cucsa.219910Cucumber (Chinese Long) v2cgycuB318
Cucsa.219910Cucumber (Chinese Long) v2cgycuB320
Cucsa.219910Cucumber (Chinese Long) v2cgycuB321
Cucsa.219910Melon (DHL92) v3.5.1cgymeB380
Cucsa.219910Melon (DHL92) v3.5.1cgymeB381
Cucsa.219910Melon (DHL92) v3.5.1cgymeB382
Cucsa.219910Melon (DHL92) v3.5.1cgymeB384
Cucsa.219910Watermelon (Charleston Gray)cgywcgB394
Cucsa.219910Watermelon (Charleston Gray)cgywcgB397
Cucsa.219910Watermelon (Charleston Gray)cgywcgB399
Cucsa.219910Watermelon (97103) v1cgywmB422
Cucsa.219910Watermelon (97103) v1cgywmB423
Cucsa.219910Watermelon (97103) v1cgywmB425
Cucsa.219910Cucurbita pepo (Zucchini)cgycpeB0593
Cucsa.219910Cucurbita pepo (Zucchini)cgycpeB0599
Cucsa.219910Bottle gourd (USVL1VR-Ls)cgylsiB362
Cucsa.219910Bottle gourd (USVL1VR-Ls)cgylsiB369
Cucsa.219910Bottle gourd (USVL1VR-Ls)cgylsiB370
Cucsa.219910Melon (DHL92) v3.6.1cgymedB378
Cucsa.219910Melon (DHL92) v3.6.1cgymedB379
Cucsa.219910Melon (DHL92) v3.6.1cgymedB380
Cucsa.219910Melon (DHL92) v3.6.1cgymedB382
Cucsa.219910Silver-seed gourdcarcgyB0251
Cucsa.219910Silver-seed gourdcarcgyB0434
Cucsa.219910Silver-seed gourdcarcgyB0873
Cucsa.219910Silver-seed gourdcarcgyB0920