Cp4.1LG04g08070 (gene) Cucurbita pepo (Zucchini)

Overview
NameCp4.1LG04g08070
Typegene
OrganismCucurbita pepo (Cucurbita pepo (Zucchini))
DescriptionCalcium-binding EF-hand family protein
LocationCp4.1LG04 : 3425690 .. 3426244 (-)
Genome Viewer
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGCCGATGAACCCAAATCTCCTCGACCTGCAATACAGAGTCTCCAAGAAAAAGTTCCTCCGGGAGCCCTCCCGACTGTTCTCCTTCAAGGACCGGCAGAGCTCCGGCGTCCGGCGGGCTCTGCAACCGAGCACGGAGGAGATCAAGCAGATCTTCAACAAGTTCGACACGAACAGAGATGGGCTGATCTCAAAGCACGAATACAGGGGAGTTCTTAAGGCGCTGGGGAGAGGGAACTCGATGGAGGAGGTGCAGAAGATTTTCAGGGCGGTGGATACCGACGGCGATGGGTACATAAACTTGAAGGAGTTCATGGAGGTTCACCAGAGTGGGGACGGAGTTCAGGCGAGAGATGTGGAATTTGCTTTTAAGACGTTTGATTTGAATGGGGATCGGAAGATTAGTGCAGAGGAAATTATGAGAGTGTTGAGGGGATTAGGGGAAAAGTGTAGCATTGAGGATTGCCGGCGGATGGTGAGGGCGGTGGACAGCGACGGCGACGGTATGGTGGACATTGATGAGTTCATGACTATGATGACTCGCTCTGTGATGTAA

mRNA sequence

ATGCCGATGAACCCAAATCTCCTCGACCTGCAATACAGAGTCTCCAAGAAAAAGTTCCTCCGGGAGCCCTCCCGACTGTTCTCCTTCAAGGACCGGCAGAGCTCCGGCGTCCGGCGGGCTCTGCAACCGAGCACGGAGGAGATCAAGCAGATCTTCAACAAGTTCGACACGAACAGAGATGGGCTGATCTCAAAGCACGAATACAGGGGAGTTCTTAAGGCGCTGGGGAGAGGGAACTCGATGGAGGAGGTGCAGAAGATTTTCAGGGCGGTGGATACCGACGGCGATGGGTACATAAACTTGAAGGAGTTCATGGAGGTTCACCAGAGTGGGGACGGAGTTCAGGCGAGAGATGTGGAATTTGCTTTTAAGACGTTTGATTTGAATGGGGATCGGAAGATTAGTGCAGAGGAAATTATGAGAGTGTTGAGGGGATTAGGGGAAAAGTGTAGCATTGAGGATTGCCGGCGGATGGTGAGGGCGGTGGACAGCGACGGCGACGGTATGGTGGACATTGATGAGTTCATGACTATGATGACTCGCTCTGTGATGTAA

Coding sequence (CDS)

ATGCCGATGAACCCAAATCTCCTCGACCTGCAATACAGAGTCTCCAAGAAAAAGTTCCTCCGGGAGCCCTCCCGACTGTTCTCCTTCAAGGACCGGCAGAGCTCCGGCGTCCGGCGGGCTCTGCAACCGAGCACGGAGGAGATCAAGCAGATCTTCAACAAGTTCGACACGAACAGAGATGGGCTGATCTCAAAGCACGAATACAGGGGAGTTCTTAAGGCGCTGGGGAGAGGGAACTCGATGGAGGAGGTGCAGAAGATTTTCAGGGCGGTGGATACCGACGGCGATGGGTACATAAACTTGAAGGAGTTCATGGAGGTTCACCAGAGTGGGGACGGAGTTCAGGCGAGAGATGTGGAATTTGCTTTTAAGACGTTTGATTTGAATGGGGATCGGAAGATTAGTGCAGAGGAAATTATGAGAGTGTTGAGGGGATTAGGGGAAAAGTGTAGCATTGAGGATTGCCGGCGGATGGTGAGGGCGGTGGACAGCGACGGCGACGGTATGGTGGACATTGATGAGTTCATGACTATGATGACTCGCTCTGTGATGTAA

Protein sequence

MPMNPNLLDLQYRVSKKKFLREPSRLFSFKDRQSSGVRRALQPSTEEIKQIFNKFDTNRDGLISKHEYRGVLKALGRGNSMEEVQKIFRAVDTDGDGYINLKEFMEVHQSGDGVQARDVEFAFKTFDLNGDRKISAEEIMRVLRGLGEKCSIEDCRRMVRAVDSDGDGMVDIDEFMTMMTRSVM
Homology
[Close]
BLAST of Cp4.1LG04g08070 vs. Swiss-Prot
Match: CML1_ARATH (Calmodulin-like protein 1 OS=Arabidopsis thaliana GN=CML1 PE=2 SV=1)

HSP 1 Score: 199.1 bits (505), Expect = 4.1e-50
Identity = 97/182 (53.30%), Postives = 143/182 (78.57%), Query Frame = 1 

Query: 4   NPNLLDLQYRVSKKKFLREPSRLFSFKDRQSSGVRRA-----LQPSTEEIKQIFNKFDTN 63
           N + L+LQY++SK K LR+PSR+FS +DRQSSG+         QPS  E++++F++FD +
Sbjct: 3   NVSFLELQYKLSKNKMLRKPSRMFS-RDRQSSGLSSPGPGGFSQPSVNEMRRVFSRFDLD 62

Query: 64  RDGLISKHEYRGVLKALGRGNSMEEVQKIFRAVDTDGDGYINLKEFMEVHQSGDGVQARD 123
           +DG IS+ EY+ VL+ALG+  ++E+V KIF+AVD DGDG+I+ +EF++ ++   G+++ D
Sbjct: 63  KDGKISQTEYKVVLRALGQERAIEDVPKIFKAVDLDGDGFIDFREFIDAYKRSGGIRSSD 122

Query: 124 VEFAFKTFDLNGDRKISAEEIMRVLRGLGEKCSIEDCRRMVRAVDSDGDGMVDIDEFMTM 181
           +  +F TFDLNGD KISAEE+M VL  LGE+CS+EDC RMVRAVD+DGDG+V+++EF+ M
Sbjct: 123 IRNSFWTFDLNGDGKISAEEVMSVLWKLGERCSLEDCNRMVRAVDADGDGLVNMEEFIKM 182

[Close]
BLAST of Cp4.1LG04g08070 vs. Swiss-Prot
Match: CML23_ARATH (Probable calcium-binding protein CML23 OS=Arabidopsis thaliana GN=CML23 PE=2 SV=1)

HSP 1 Score: 127.9 bits (320), Expect = 1.2e-28
Identity = 67/150 (44.67%), Postives = 95/150 (63.33%), Query Frame = 1 

Query: 34  SSGVRRALQPSTEEIKQIFNKFDTNRDGLISKHEYRGVLKALGRGNSMEEVQKIFRAVDT 93
           S  V R    S E+IK++F +FD N DG IS  E + V+ AL    S EE + + +  D 
Sbjct: 2   SKNVSRNCLGSMEDIKKVFQRFDKNNDGKISIDELKDVIGALSPNASQEETKAMMKEFDL 61

Query: 94  DGDGYINLKEFMEVHQ----SGDGVQARDVEFAFKTFDLNGDRKISAEEIMRVLRGLGEK 153
           DG+G+I+L EF+ + Q    S +    RD++ AF  +DL+ + +ISA E+  V++ LGEK
Sbjct: 62  DGNGFIDLDEFVALFQISDQSSNNSAIRDLKEAFDLYDLDRNGRISANELHSVMKNLGEK 121

Query: 154 CSIEDCRRMVRAVDSDGDGMVDIDEFMTMM 180
           CSI+DC+RM+  VDSDGDG VD +EF  MM
Sbjct: 122 CSIQDCQRMINKVDSDGDGCVDFEEFKKMM 151

[Close]
BLAST of Cp4.1LG04g08070 vs. Swiss-Prot
Match: CML24_ARATH (Calcium-binding protein CML24 OS=Arabidopsis thaliana GN=CML24 PE=2 SV=2)

HSP 1 Score: 124.8 bits (312), Expect = 9.9e-28
Identity = 64/152 (42.11%), Postives = 99/152 (65.13%), Query Frame = 1 

Query: 35  SGVRRALQPSTEEIKQIFNKFDTNRDGLISKHEYRGVLKALGRGNSMEEVQKIFRAVDTD 94
           +GV R+   S ++IK++F +FD N DG IS  E + V++AL    S EE   + +  D D
Sbjct: 5   NGVVRSCLGSMDDIKKVFQRFDKNGDGKISVDELKEVIRALSPTASPEETVTMMKQFDLD 64

Query: 95  GDGYINLKEFMEVHQSGDGV------QARDVEFAFKTFDLNGDRKISAEEIMRVLRGLGE 154
           G+G+I+L EF+ + Q G G          D++ AF+ +DL+G+ +ISA+E+  V++ LGE
Sbjct: 65  GNGFIDLDEFVALFQIGIGGGGNNRNDVSDLKEAFELYDLDGNGRISAKELHSVMKNLGE 124

Query: 155 KCSIEDCRRMVRAVDSDGDGMVDIDEFMTMMT 181
           KCS++DC++M+  VD DGDG V+ DEF  MM+
Sbjct: 125 KCSVQDCKKMISKVDIDGDGCVNFDEFKKMMS 156

[Close]
BLAST of Cp4.1LG04g08070 vs. Swiss-Prot
Match: CML25_ARATH (Probable calcium-binding protein CML25 OS=Arabidopsis thaliana GN=CML25 PE=2 SV=1)

HSP 1 Score: 123.2 bits (308), Expect = 2.9e-27
Identity = 60/144 (41.67%), Postives = 90/144 (62.50%), Query Frame = 1 

Query: 39  RALQPSTEEIKQIFNKFDTNRDGLISKHEYRGVLKALGRGNSMEEVQKIFRAVDTDGDGY 98
           R+ +    E++ +F KFD N DG IS  E   ++ +LG     EE++K    +D  GDGY
Sbjct: 29  RSGKTEIRELEAVFKKFDVNGDGKISSKELGAIMTSLGHEVPEEELEKAITEIDRKGDGY 88

Query: 99  INLKEFMEVHQSG--DGVQARDVEFAFKTFDLNGDRKISAEEIMRVLRGLGEKCSIEDCR 158
           IN +EF+E++  G        +++ AF  +D++G+  ISAEE+  VLR LG++CSI +CR
Sbjct: 89  INFEEFVELNTKGMDQNDVLENLKDAFSVYDIDGNGSISAEELHEVLRSLGDECSIAECR 148

Query: 159 RMVRAVDSDGDGMVDIDEFMTMMT 181
           +M+  VD DGDG +D +EF  MMT
Sbjct: 149 KMIGGVDKDGDGTIDFEEFKIMMT 172

[Close]
BLAST of Cp4.1LG04g08070 vs. Swiss-Prot
Match: CML10_ORYSJ (Probable calcium-binding protein CML10 OS=Oryza sativa subsp. japonica GN=CML10 PE=2 SV=1)

HSP 1 Score: 121.3 bits (303), Expect = 1.1e-26
Identity = 62/152 (40.79%), Postives = 93/152 (61.18%), Query Frame = 1 

Query: 31  DRQSSGVRRALQPSTEEIKQIFNKFDTNRDGLISKHEYRGVLKALGRGNSMEEVQKIFRA 90
           D  S G   A  P  EE++++F KFD N DG IS+ E   + ++LG   + +E+ ++   
Sbjct: 25  DPASGGGSPAPTPE-EEMERVFRKFDANGDGRISRSELGALFESLGHAATDDELARMMAE 84

Query: 91  VDTDGDGYINLKEFMEVHQSGDGVQA---RDVEFAFKTFDLNGDRKISAEEIMRVLRGLG 150
            D DGDG+I+L EF  ++ +  G  A    D+  AF+ FD +G+  ISA E+ RVL GLG
Sbjct: 85  ADADGDGFISLDEFAALNATASGDAAAVEEDLRHAFRVFDADGNGTISAAELARVLHGLG 144

Query: 151 EKCSIEDCRRMVRAVDSDGDGMVDIDEFMTMM 180
           EK +++ CRRM+  VD +GDG++  +EF  MM
Sbjct: 145 EKATVQQCRRMIEGVDQNGDGLISFEEFKVMM 175

[Close]
BLAST of Cp4.1LG04g08070 vs. TrEMBL
Match: A0A0A0K804_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_7G073720 PE=4 SV=1)

HSP 1 Score: 335.1 bits (858), Expect = 5.3e-89
Identity = 164/182 (90.11%), Postives = 177/182 (97.25%), Query Frame = 1 

Query: 1   MPMNPNLLDLQYRVSKKKFLREPSRLFSFKDRQSSGVRRALQPSTEEIKQIFNKFDTNRD 60
           MP+NPN L+LQY VSKKKFLREPSRLFSFKDRQSSG+RRALQPS+EEIKQIFNKFDTN+D
Sbjct: 1   MPINPNFLELQYNVSKKKFLREPSRLFSFKDRQSSGLRRALQPSSEEIKQIFNKFDTNKD 60

Query: 61  GLISKHEYRGVLKALGRGNSMEEVQKIFRAVDTDGDGYINLKEFMEVHQSGDGVQARDVE 120
           G ISKHEYRG+LKALGRGNSMEEVQKIFRAVD+DGDGYINL EFMEVH+SG GVQA++VE
Sbjct: 61  GRISKHEYRGILKALGRGNSMEEVQKIFRAVDSDGDGYINLNEFMEVHRSGGGVQAKEVE 120

Query: 121 FAFKTFDLNGDRKISAEEIMRVLRGLGEKCSIEDCRRMVRAVDSDGDGMVDIDEFMTMMT 180
           FAFKTFDLNGDRKISAEE+MRVL+GLGEKCSIEDCRRMVRAVDSDGDGMVDI+EFMTMMT
Sbjct: 121 FAFKTFDLNGDRKISAEEVMRVLKGLGEKCSIEDCRRMVRAVDSDGDGMVDINEFMTMMT 180

Query: 181 RS 183
           RS
Sbjct: 181 RS 182

[Close]
BLAST of Cp4.1LG04g08070 vs. TrEMBL
Match: M5VNS5_PRUPE (Uncharacterized protein OS=Prunus persica GN=PRUPE_ppa011990mg PE=4 SV=1)

HSP 1 Score: 247.3 bits (630), Expect = 1.5e-62
Identity = 115/178 (64.61%), Postives = 149/178 (83.71%), Query Frame = 1 

Query: 6   NLLDLQYRVSKKKFLREPSRLFSFKDRQSSGVRRALQPSTEEIKQIFNKFDTNRDGLISK 65
           N LD QY +SK+KFLR+PS +FSF+DRQ+SGV  + +P+  E+K++F+KFD+N+DG IS+
Sbjct: 5   NFLDFQYNLSKRKFLRKPSHVFSFRDRQNSGVSPSFEPNPNEMKRVFDKFDSNKDGKISQ 64

Query: 66  HEYRGVLKALGRGNSMEEVQKIFRAVDTDGDGYINLKEFMEVHQSGDGVQARDVEFAFKT 125
            EY+ +L+ALG+GN + EV KIFR  D DGDG+IN KEFMEVH+ G G++  D++ AF+T
Sbjct: 65  PEYKAILRALGKGNMVGEVPKIFRIADLDGDGFINFKEFMEVHKKGGGIRTIDIQNAFRT 124

Query: 126 FDLNGDRKISAEEIMRVLRGLGEKCSIEDCRRMVRAVDSDGDGMVDIDEFMTMMTRSV 184
           FDLNGD KISAEE+M VL  LGE CS+EDC+RMVRAVD+DGDG+VDIDEFMTMMTRS+
Sbjct: 125 FDLNGDGKISAEEVMEVLGRLGESCSLEDCQRMVRAVDTDGDGVVDIDEFMTMMTRSM 182

[Close]
BLAST of Cp4.1LG04g08070 vs. TrEMBL
Match: B9HS04_POPTR (Calmodulin-related family protein OS=Populus trichocarpa GN=POPTR_0009s10530g PE=4 SV=1)

HSP 1 Score: 235.7 bits (600), Expect = 4.4e-59
Identity = 110/180 (61.11%), Postives = 147/180 (81.67%), Query Frame = 1 

Query: 4   NPNLLDLQYRVSKKKFLREPSRLFSFKDRQSSGVRRALQPSTEEIKQIFNKFDTNRDGLI 63
           N + L+ QY++S+ KFLR+PSRLFS +DRQSSG+    QP+ +E++Q+F+KFD+N+DG I
Sbjct: 3   NLSFLEFQYKLSRNKFLRKPSRLFSSRDRQSSGMLSTFQPNMKEMRQVFDKFDSNKDGKI 62

Query: 64  SKHEYRGVLKALGRGNSMEEVQKIFRAVDTDGDGYINLKEFMEVHQSGDGVQARDVEFAF 123
           S+ EY+  L+ALG+GN + EV KIF+ VD DGDG+I+ KEF+E  + G G++  D++ AF
Sbjct: 63  SQQEYKDTLRALGQGNMLGEVPKIFQVVDLDGDGFIDFKEFVEAQKKGGGIRTTDIQTAF 122

Query: 124 KTFDLNGDRKISAEEIMRVLRGLGEKCSIEDCRRMVRAVDSDGDGMVDIDEFMTMMTRSV 183
           +TFD NGD KISAEE+M VLR LGE+CS+EDCRRMV AVD DGDGMV++DEFMTMMTRS+
Sbjct: 123 QTFDSNGDGKISAEEVMEVLRRLGERCSLEDCRRMVNAVDIDGDGMVNMDEFMTMMTRSM 182

[Close]
BLAST of Cp4.1LG04g08070 vs. TrEMBL
Match: A0A067F0V1_CITSI (Uncharacterized protein OS=Citrus sinensis GN=CISIN_1g041237mg PE=4 SV=1)

HSP 1 Score: 227.6 bits (579), Expect = 1.2e-56
Identity = 105/180 (58.33%), Postives = 144/180 (80.00%), Query Frame = 1 

Query: 4   NPNLLDLQYRVSKKKFLREPSRLFSFKDRQSSGVRRALQPSTEEIKQIFNKFDTNRDGLI 63
           N + LD QY++SK KFLR+PSRLFS +DRQ+S +    Q    E+K++F++FD+N+DG I
Sbjct: 3   NLSFLDFQYKISKNKFLRKPSRLFSSRDRQNSSLLPTFQADRNEMKRVFDRFDSNKDGKI 62

Query: 64  SKHEYRGVLKALGRGNSMEEVQKIFRAVDTDGDGYINLKEFMEVHQSGDGVQARDVEFAF 123
           S+ EY+ +L+A+G+ + + EV KIF+ VD DGDG+I+ KEFME H+ G G++  D++ AF
Sbjct: 63  SQMEYKAILRAIGQASMISEVPKIFQVVDLDGDGFIDFKEFMEAHKKGGGIRTMDIQSAF 122

Query: 124 KTFDLNGDRKISAEEIMRVLRGLGEKCSIEDCRRMVRAVDSDGDGMVDIDEFMTMMTRSV 183
           +TFD N D KISAEEI+ +LR LGE CS+EDCR+MVRAVD+DGDGMV++DEFMTMMTRS+
Sbjct: 123 RTFDKNDDGKISAEEILEMLRRLGESCSLEDCRKMVRAVDTDGDGMVNMDEFMTMMTRSM 182

[Close]
BLAST of Cp4.1LG04g08070 vs. TrEMBL
Match: V4SBR8_9ROSI (Uncharacterized protein OS=Citrus clementina GN=CICLE_v10029360mg PE=4 SV=1)

HSP 1 Score: 227.6 bits (579), Expect = 1.2e-56
Identity = 105/180 (58.33%), Postives = 144/180 (80.00%), Query Frame = 1 

Query: 4   NPNLLDLQYRVSKKKFLREPSRLFSFKDRQSSGVRRALQPSTEEIKQIFNKFDTNRDGLI 63
           N + LD QY++SK KFLR+PSRLFS +DRQ+S +    Q    E+K++F++FD+N+DG I
Sbjct: 3   NLSFLDFQYKISKNKFLRKPSRLFSSRDRQNSSLLPTFQADRNEMKRVFDRFDSNKDGKI 62

Query: 64  SKHEYRGVLKALGRGNSMEEVQKIFRAVDTDGDGYINLKEFMEVHQSGDGVQARDVEFAF 123
           S+ EY+ +L+A+G+ + + EV KIF+ VD DGDG+I+ KEFME H+ G G++  D++ AF
Sbjct: 63  SQMEYKAILRAIGQASMISEVPKIFQVVDLDGDGFIDFKEFMEAHKKGGGIRTMDIQSAF 122

Query: 124 KTFDLNGDRKISAEEIMRVLRGLGEKCSIEDCRRMVRAVDSDGDGMVDIDEFMTMMTRSV 183
           +TFD N D KISAEEI+ +LR LGE CS+EDCR+MVRAVD+DGDGMV++DEFMTMMTRS+
Sbjct: 123 RTFDKNDDGKISAEEILEMLRRLGESCSLEDCRKMVRAVDTDGDGMVNMDEFMTMMTRSM 182

[Close]
BLAST of Cp4.1LG04g08070 vs. TAIR10
Match: AT2G15680.1 (AT2G15680.1 Calcium-binding EF-hand family protein)

HSP 1 Score: 199.1 bits (505), Expect = 2.3e-51
Identity = 97/182 (53.30%), Postives = 143/182 (78.57%), Query Frame = 1 

Query: 4   NPNLLDLQYRVSKKKFLREPSRLFSFKDRQSSGVRRA-----LQPSTEEIKQIFNKFDTN 63
           N + L+LQY++SK K LR+PSR+FS +DRQSSG+         QPS  E++++F++FD +
Sbjct: 3   NVSFLELQYKLSKNKMLRKPSRMFS-RDRQSSGLSSPGPGGFSQPSVNEMRRVFSRFDLD 62

Query: 64  RDGLISKHEYRGVLKALGRGNSMEEVQKIFRAVDTDGDGYINLKEFMEVHQSGDGVQARD 123
           +DG IS+ EY+ VL+ALG+  ++E+V KIF+AVD DGDG+I+ +EF++ ++   G+++ D
Sbjct: 63  KDGKISQTEYKVVLRALGQERAIEDVPKIFKAVDLDGDGFIDFREFIDAYKRSGGIRSSD 122

Query: 124 VEFAFKTFDLNGDRKISAEEIMRVLRGLGEKCSIEDCRRMVRAVDSDGDGMVDIDEFMTM 181
           +  +F TFDLNGD KISAEE+M VL  LGE+CS+EDC RMVRAVD+DGDG+V+++EF+ M
Sbjct: 123 IRNSFWTFDLNGDGKISAEEVMSVLWKLGERCSLEDCNRMVRAVDADGDGLVNMEEFIKM 182

[Close]
BLAST of Cp4.1LG04g08070 vs. TAIR10
Match: AT1G66400.1 (AT1G66400.1 calmodulin like 23)

HSP 1 Score: 127.9 bits (320), Expect = 6.6e-30
Identity = 67/150 (44.67%), Postives = 95/150 (63.33%), Query Frame = 1 

Query: 34  SSGVRRALQPSTEEIKQIFNKFDTNRDGLISKHEYRGVLKALGRGNSMEEVQKIFRAVDT 93
           S  V R    S E+IK++F +FD N DG IS  E + V+ AL    S EE + + +  D 
Sbjct: 2   SKNVSRNCLGSMEDIKKVFQRFDKNNDGKISIDELKDVIGALSPNASQEETKAMMKEFDL 61

Query: 94  DGDGYINLKEFMEVHQ----SGDGVQARDVEFAFKTFDLNGDRKISAEEIMRVLRGLGEK 153
           DG+G+I+L EF+ + Q    S +    RD++ AF  +DL+ + +ISA E+  V++ LGEK
Sbjct: 62  DGNGFIDLDEFVALFQISDQSSNNSAIRDLKEAFDLYDLDRNGRISANELHSVMKNLGEK 121

Query: 154 CSIEDCRRMVRAVDSDGDGMVDIDEFMTMM 180
           CSI+DC+RM+  VDSDGDG VD +EF  MM
Sbjct: 122 CSIQDCQRMINKVDSDGDGCVDFEEFKKMM 151

[Close]
BLAST of Cp4.1LG04g08070 vs. TAIR10
Match: AT5G37770.1 (AT5G37770.1 EF hand calcium-binding protein family)

HSP 1 Score: 124.8 bits (312), Expect = 5.6e-29
Identity = 64/152 (42.11%), Postives = 99/152 (65.13%), Query Frame = 1 

Query: 35  SGVRRALQPSTEEIKQIFNKFDTNRDGLISKHEYRGVLKALGRGNSMEEVQKIFRAVDTD 94
           +GV R+   S ++IK++F +FD N DG IS  E + V++AL    S EE   + +  D D
Sbjct: 5   NGVVRSCLGSMDDIKKVFQRFDKNGDGKISVDELKEVIRALSPTASPEETVTMMKQFDLD 64

Query: 95  GDGYINLKEFMEVHQSGDGV------QARDVEFAFKTFDLNGDRKISAEEIMRVLRGLGE 154
           G+G+I+L EF+ + Q G G          D++ AF+ +DL+G+ +ISA+E+  V++ LGE
Sbjct: 65  GNGFIDLDEFVALFQIGIGGGGNNRNDVSDLKEAFELYDLDGNGRISAKELHSVMKNLGE 124

Query: 155 KCSIEDCRRMVRAVDSDGDGMVDIDEFMTMMT 181
           KCS++DC++M+  VD DGDG V+ DEF  MM+
Sbjct: 125 KCSVQDCKKMISKVDIDGDGCVNFDEFKKMMS 156

[Close]
BLAST of Cp4.1LG04g08070 vs. TAIR10
Match: AT1G24620.1 (AT1G24620.1 EF hand calcium-binding protein family)

HSP 1 Score: 123.2 bits (308), Expect = 1.6e-28
Identity = 60/144 (41.67%), Postives = 90/144 (62.50%), Query Frame = 1 

Query: 39  RALQPSTEEIKQIFNKFDTNRDGLISKHEYRGVLKALGRGNSMEEVQKIFRAVDTDGDGY 98
           R+ +    E++ +F KFD N DG IS  E   ++ +LG     EE++K    +D  GDGY
Sbjct: 29  RSGKTEIRELEAVFKKFDVNGDGKISSKELGAIMTSLGHEVPEEELEKAITEIDRKGDGY 88

Query: 99  INLKEFMEVHQSG--DGVQARDVEFAFKTFDLNGDRKISAEEIMRVLRGLGEKCSIEDCR 158
           IN +EF+E++  G        +++ AF  +D++G+  ISAEE+  VLR LG++CSI +CR
Sbjct: 89  INFEEFVELNTKGMDQNDVLENLKDAFSVYDIDGNGSISAEELHEVLRSLGDECSIAECR 148

Query: 159 RMVRAVDSDGDGMVDIDEFMTMMT 181
           +M+  VD DGDG +D +EF  MMT
Sbjct: 149 KMIGGVDKDGDGTIDFEEFKIMMT 172

[Close]
BLAST of Cp4.1LG04g08070 vs. TAIR10
Match: AT1G18210.1 (AT1G18210.1 Calcium-binding EF-hand family protein)

HSP 1 Score: 120.9 bits (302), Expect = 8.0e-28
Identity = 63/137 (45.99%), Postives = 88/137 (64.23%), Query Frame = 1 

Query: 46  EEIKQIFNKFDTNRDGLISKHEYRGVLKALGRGNSMEEVQKIFRAVDTDGDGYINLKEFM 105
           EE+K++F++FD+N DG IS  E  GV KA+G   +  E+ ++   VDTD DGYINL EF 
Sbjct: 22  EELKKVFDQFDSNGDGKISVLELGGVFKAMGTSYTETELNRVLEEVDTDRDGYINLDEFS 81

Query: 106 EVHQSGDGVQARDVEFAFKTFDLNGDRKISAEEIMRVLRGLGEKCSIEDCRRMVRAVDSD 165
            + +S     A ++  AF  +D + +  ISA E+ +VL  LG  CS+EDC RM+  VD+D
Sbjct: 82  TLCRSSSS--AAEIRDAFDLYDQDKNGLISASELHQVLNRLGMSCSVEDCTRMIGPVDAD 141

Query: 166 GDGMVDIDEFMTMMTRS 183
           GDG V+ +EF  MMT S
Sbjct: 142 GDGNVNFEEFQKMMTSS 156

[Close]
BLAST of Cp4.1LG04g08070 vs. NCBI nr
Match: gi|778725125|ref|XP_004137114.2| (PREDICTED: calmodulin-like protein 1 [Cucumis sativus])

HSP 1 Score: 335.1 bits (858), Expect = 7.7e-89
Identity = 164/182 (90.11%), Postives = 177/182 (97.25%), Query Frame = 1 

Query: 1   MPMNPNLLDLQYRVSKKKFLREPSRLFSFKDRQSSGVRRALQPSTEEIKQIFNKFDTNRD 60
           MP+NPN L+LQY VSKKKFLREPSRLFSFKDRQSSG+RRALQPS+EEIKQIFNKFDTN+D
Sbjct: 1   MPINPNFLELQYNVSKKKFLREPSRLFSFKDRQSSGLRRALQPSSEEIKQIFNKFDTNKD 60

Query: 61  GLISKHEYRGVLKALGRGNSMEEVQKIFRAVDTDGDGYINLKEFMEVHQSGDGVQARDVE 120
           G ISKHEYRG+LKALGRGNSMEEVQKIFRAVD+DGDGYINL EFMEVH+SG GVQA++VE
Sbjct: 61  GRISKHEYRGILKALGRGNSMEEVQKIFRAVDSDGDGYINLNEFMEVHRSGGGVQAKEVE 120

Query: 121 FAFKTFDLNGDRKISAEEIMRVLRGLGEKCSIEDCRRMVRAVDSDGDGMVDIDEFMTMMT 180
           FAFKTFDLNGDRKISAEE+MRVL+GLGEKCSIEDCRRMVRAVDSDGDGMVDI+EFMTMMT
Sbjct: 121 FAFKTFDLNGDRKISAEEVMRVLKGLGEKCSIEDCRRMVRAVDSDGDGMVDINEFMTMMT 180

Query: 181 RS 183
           RS
Sbjct: 181 RS 182

[Close]
BLAST of Cp4.1LG04g08070 vs. NCBI nr
Match: gi|659109907|ref|XP_008454944.1| (PREDICTED: calmodulin-like protein 1 [Cucumis melo])

HSP 1 Score: 334.0 bits (855), Expect = 1.7e-88
Identity = 164/182 (90.11%), Postives = 176/182 (96.70%), Query Frame = 1 

Query: 1   MPMNPNLLDLQYRVSKKKFLREPSRLFSFKDRQSSGVRRALQPSTEEIKQIFNKFDTNRD 60
           MP+NPN LDLQY VSKKKFLREPSRLFSFKDRQSSG+RRALQPS EEIKQIFNKFDTN+D
Sbjct: 1   MPINPNFLDLQYNVSKKKFLREPSRLFSFKDRQSSGLRRALQPSLEEIKQIFNKFDTNKD 60

Query: 61  GLISKHEYRGVLKALGRGNSMEEVQKIFRAVDTDGDGYINLKEFMEVHQSGDGVQARDVE 120
           G ISKHEYRG+LKALGRG+SMEEVQKIFRAVD+DGDGYINL EFMEVH+SG GVQA++VE
Sbjct: 61  GRISKHEYRGILKALGRGSSMEEVQKIFRAVDSDGDGYINLNEFMEVHRSGGGVQAKEVE 120

Query: 121 FAFKTFDLNGDRKISAEEIMRVLRGLGEKCSIEDCRRMVRAVDSDGDGMVDIDEFMTMMT 180
           FAFKTFDLNGDRKISAEE+MRVL+GLGEKCSIEDCRRMVRAVDSDGDGMVDI+EFMTMMT
Sbjct: 121 FAFKTFDLNGDRKISAEEVMRVLKGLGEKCSIEDCRRMVRAVDSDGDGMVDINEFMTMMT 180

Query: 181 RS 183
           RS
Sbjct: 181 RS 182

[Close]
BLAST of Cp4.1LG04g08070 vs. NCBI nr
Match: gi|595798018|ref|XP_007201342.1| (hypothetical protein PRUPE_ppa011990mg [Prunus persica])

HSP 1 Score: 247.3 bits (630), Expect = 2.1e-62
Identity = 115/178 (64.61%), Postives = 149/178 (83.71%), Query Frame = 1 

Query: 6   NLLDLQYRVSKKKFLREPSRLFSFKDRQSSGVRRALQPSTEEIKQIFNKFDTNRDGLISK 65
           N LD QY +SK+KFLR+PS +FSF+DRQ+SGV  + +P+  E+K++F+KFD+N+DG IS+
Sbjct: 5   NFLDFQYNLSKRKFLRKPSHVFSFRDRQNSGVSPSFEPNPNEMKRVFDKFDSNKDGKISQ 64

Query: 66  HEYRGVLKALGRGNSMEEVQKIFRAVDTDGDGYINLKEFMEVHQSGDGVQARDVEFAFKT 125
            EY+ +L+ALG+GN + EV KIFR  D DGDG+IN KEFMEVH+ G G++  D++ AF+T
Sbjct: 65  PEYKAILRALGKGNMVGEVPKIFRIADLDGDGFINFKEFMEVHKKGGGIRTIDIQNAFRT 124

Query: 126 FDLNGDRKISAEEIMRVLRGLGEKCSIEDCRRMVRAVDSDGDGMVDIDEFMTMMTRSV 184
           FDLNGD KISAEE+M VL  LGE CS+EDC+RMVRAVD+DGDG+VDIDEFMTMMTRS+
Sbjct: 125 FDLNGDGKISAEEVMEVLGRLGESCSLEDCQRMVRAVDTDGDGVVDIDEFMTMMTRSM 182

[Close]
BLAST of Cp4.1LG04g08070 vs. NCBI nr
Match: gi|645261897|ref|XP_008236514.1| (PREDICTED: calmodulin-like protein 1 [Prunus mume])

HSP 1 Score: 245.4 bits (625), Expect = 8.0e-62
Identity = 113/178 (63.48%), Postives = 148/178 (83.15%), Query Frame = 1 

Query: 6   NLLDLQYRVSKKKFLREPSRLFSFKDRQSSGVRRALQPSTEEIKQIFNKFDTNRDGLISK 65
           N LD Q+ +SK+KFLR+PS + SF+DRQ+SG+  + +P+  E+K +F KFD+N+DG IS+
Sbjct: 5   NFLDFQFNLSKRKFLRKPSHVCSFRDRQNSGLSPSFEPNPNEMKIVFEKFDSNKDGKISQ 64

Query: 66  HEYRGVLKALGRGNSMEEVQKIFRAVDTDGDGYINLKEFMEVHQSGDGVQARDVEFAFKT 125
            EY+ +L+ALG+GN++ EV KIFR  D DGDG+IN KEFMEVH+ G G++  D++ AF+T
Sbjct: 65  EEYKAILRALGKGNTVREVPKIFRIADLDGDGFINFKEFMEVHKKGGGIRTMDIQNAFRT 124

Query: 126 FDLNGDRKISAEEIMRVLRGLGEKCSIEDCRRMVRAVDSDGDGMVDIDEFMTMMTRSV 184
           FDLNGD KISAEE+M VLR LGE CS+EDC+RMVRAVD+DGDG+VDIDEFMTMMTRS+
Sbjct: 125 FDLNGDGKISAEEVMEVLRRLGESCSLEDCQRMVRAVDTDGDGVVDIDEFMTMMTRSM 182

[Close]
BLAST of Cp4.1LG04g08070 vs. NCBI nr
Match: gi|657963113|ref|XP_008373157.1| (PREDICTED: calmodulin-like protein 1 [Malus domestica])

HSP 1 Score: 243.8 bits (621), Expect = 2.3e-61
Identity = 114/180 (63.33%), Postives = 149/180 (82.78%), Query Frame = 1 

Query: 4   NPNLLDLQYRVSKKKFLREPSRLFSFKDRQSSGVRRALQPSTEEIKQIFNKFDTNRDGLI 63
           N N LD QY +SK+KFLR+PS++FSF+DRQ SG+    +P+  E+K++F KFD+N+DG I
Sbjct: 3   NANFLDFQYNLSKRKFLRKPSQMFSFRDRQYSGIAPTFEPNPNEMKKVFEKFDSNKDGKI 62

Query: 64  SKHEYRGVLKALGRGNSMEEVQKIFRAVDTDGDGYINLKEFMEVHQSGDGVQARDVEFAF 123
           S+ EY+ +L+ALG+GN + EV KIF+ VD D DG+IN KEFMEVH+ G G++  D++ AF
Sbjct: 63  SQAEYKAILRALGKGNMIGEVPKIFKFVDLDEDGFINFKEFMEVHKKG-GIRTMDIQNAF 122

Query: 124 KTFDLNGDRKISAEEIMRVLRGLGEKCSIEDCRRMVRAVDSDGDGMVDIDEFMTMMTRSV 183
           + FDLNGD KISAEE+M VLRGL E+C++EDCRRMVRAVD+DGDGMVD+DEFMTMMTRS+
Sbjct: 123 RMFDLNGDGKISAEEVMEVLRGLEERCNLEDCRRMVRAVDTDGDGMVDLDEFMTMMTRSM 181

The following BLAST results are available for this feature:
BLAST of Cp4.1LG04g08070 vs. Swiss-Prot
Match NameE-valueIdentityDescription
CML1_ARATH4.1e-5053.30Calmodulin-like protein 1 OS=Arabidopsis thaliana GN=CML1 PE=2 SV=1[more]
CML23_ARATH1.2e-2844.67Probable calcium-binding protein CML23 OS=Arabidopsis thaliana GN=CML23 PE=2 SV=... [more]
CML24_ARATH9.9e-2842.11Calcium-binding protein CML24 OS=Arabidopsis thaliana GN=CML24 PE=2 SV=2[more]
CML25_ARATH2.9e-2741.67Probable calcium-binding protein CML25 OS=Arabidopsis thaliana GN=CML25 PE=2 SV=... [more]
CML10_ORYSJ1.1e-2640.79Probable calcium-binding protein CML10 OS=Oryza sativa subsp. japonica GN=CML10 ... [more]
BLAST of Cp4.1LG04g08070 vs. TrEMBL
Match NameE-valueIdentityDescription
A0A0A0K804_CUCSA5.3e-8990.11Uncharacterized protein OS=Cucumis sativus GN=Csa_7G073720 PE=4 SV=1[more]
M5VNS5_PRUPE1.5e-6264.61Uncharacterized protein OS=Prunus persica GN=PRUPE_ppa011990mg PE=4 SV=1[more]
B9HS04_POPTR4.4e-5961.11Calmodulin-related family protein OS=Populus trichocarpa GN=POPTR_0009s10530g PE... [more]
A0A067F0V1_CITSI1.2e-5658.33Uncharacterized protein OS=Citrus sinensis GN=CISIN_1g041237mg PE=4 SV=1[more]
V4SBR8_9ROSI1.2e-5658.33Uncharacterized protein OS=Citrus clementina GN=CICLE_v10029360mg PE=4 SV=1[more]
BLAST of Cp4.1LG04g08070 vs. TAIR10
Match NameE-valueIdentityDescription
AT2G15680.12.3e-5153.30 Calcium-binding EF-hand family protein[more]
AT1G66400.16.6e-3044.67 calmodulin like 23[more]
AT5G37770.15.6e-2942.11 EF hand calcium-binding protein family[more]
AT1G24620.11.6e-2841.67 EF hand calcium-binding protein family[more]
AT1G18210.18.0e-2845.99 Calcium-binding EF-hand family protein[more]
BLAST of Cp4.1LG04g08070 vs. NCBI nr
Match NameE-valueIdentityDescription
gi|778725125|ref|XP_004137114.2|7.7e-8990.11PREDICTED: calmodulin-like protein 1 [Cucumis sativus][more]
gi|659109907|ref|XP_008454944.1|1.7e-8890.11PREDICTED: calmodulin-like protein 1 [Cucumis melo][more]
gi|595798018|ref|XP_007201342.1|2.1e-6264.61hypothetical protein PRUPE_ppa011990mg [Prunus persica][more]
gi|645261897|ref|XP_008236514.1|8.0e-6263.48PREDICTED: calmodulin-like protein 1 [Prunus mume][more]
gi|657963113|ref|XP_008373157.1|2.3e-6163.33PREDICTED: calmodulin-like protein 1 [Malus domestica][more]
Annotated Terms
The following terms have been associated with this gene:
Vocabulary: Molecular Function
TermDefinition
GO:0005509calcium ion binding
Vocabulary: INTERPRO
TermDefinition
IPR018247EF_Hand_1_Ca_BS
IPR011992EF-hand-dom_pair
IPR002048EF_hand_dom
GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0008150 biological_process
cellular_component GO:0005575 cellular_component
cellular_component GO:0005739 mitochondrion
molecular_function GO:0005509 calcium ion binding
Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Cp4.1LG04g08070.1Cp4.1LG04g08070.1mRNA


InterPro
Analysis Name: InterPro Annotations of Cucurbita pepo
Date Performed: 2017-12-02
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR002048EF-hand domainPFAMPF13499EF-hand_7coord: 46..106
score: 1.5E-14coord: 119..179
score: 1.1
IPR002048EF-hand domainSMARTSM00054efh_1coord: 118..146
score: 0.0024coord: 154..182
score: 0.0063coord: 83..111
score: 3.4E-5coord: 47..75
score: 3.
IPR002048EF-hand domainPROFILEPS50222EF_HAND_2coord: 43..78
score: 15.239coord: 118..149
score: 12.477coord: 79..114
score: 14.011coord: 150..184
score: 11
IPR011992EF-hand domain pairGENE3DG3DSA:1.10.238.10coord: 45..129
score: 1.2E-25coord: 130..181
score: 1.0
IPR011992EF-hand domain pairunknownSSF47473EF-handcoord: 45..181
score: 1.01
IPR018247EF-Hand 1, calcium-binding sitePROSITEPS00018EF_HAND_1coord: 163..175
score: -coord: 56..68
score: -coord: 127..139
score: -coord: 92..104
scor
NoneNo IPR availablePANTHERPTHR10891EF-HAND CALCIUM-BINDING DOMAIN CONTAINING PROTEINcoord: 42..181
score: 4.4
NoneNo IPR availablePANTHERPTHR10891:SF533SUBFAMILY NOT NAMEDcoord: 42..181
score: 4.4

Synteny
The following gene(s) are orthologous to this gene:
GeneOrthologueOrganismBlock
Cp4.1LG04g08070Cucsa.219910Cucumber (Gy14) v1cgycpeB0602
Cp4.1LG04g08070CmaCh19G006090Cucurbita maxima (Rimu)cmacpeB539
Cp4.1LG04g08070CmoCh11G013580Cucurbita moschata (Rifu)cmocpeB126
Cp4.1LG04g08070Cla016007Watermelon (97103) v1cpewmB665
Cp4.1LG04g08070Csa7G073720Cucumber (Chinese Long) v2cpecuB695
Cp4.1LG04g08070MELO3C018445Melon (DHL92) v3.5.1cpemeB604
Cp4.1LG04g08070ClCG02G005880Watermelon (Charleston Gray)cpewcgB590
Cp4.1LG04g08070CSPI07G07690Wild cucumber (PI 183967)cpecpiB697
Cp4.1LG04g08070Lsi03G004870Bottle gourd (USVL1VR-Ls)cpelsiB543
Cp4.1LG04g08070MELO3C018445.2Melon (DHL92) v3.6.1cpemedB715
Cp4.1LG04g08070CsaV3_7G008650Cucumber (Chinese Long) v3cpecucB0868
Cp4.1LG04g08070Bhi05G000996Wax gourdcpewgoB0855
Cp4.1LG04g08070CsGy7G007250Cucumber (Gy14) v2cgybcpeB969
Cp4.1LG04g08070Carg20367Silver-seed gourdcarcpeB0764
The following gene(s) are paralogous to this gene:
GeneParalogueOrganismBlock
Cp4.1LG04g08070Cp4.1LG15g04910Cucurbita pepo (Zucchini)cpecpeB269
The following block(s) are covering this gene:
GeneOrganismBlock
Cp4.1LG04g08070Cucurbita pepo (Zucchini)cpecpeB400
Cp4.1LG04g08070Cucurbita pepo (Zucchini)cpecpeB417
Cp4.1LG04g08070Cucurbita pepo (Zucchini)cpecpeB500
Cp4.1LG04g08070Cucumber (Gy14) v1cgycpeB0222
Cp4.1LG04g08070Cucurbita maxima (Rimu)cmacpeB154
Cp4.1LG04g08070Cucurbita maxima (Rimu)cmacpeB749
Cp4.1LG04g08070Cucurbita maxima (Rimu)cmacpeB884
Cp4.1LG04g08070Cucurbita moschata (Rifu)cmocpeB494
Cp4.1LG04g08070Cucurbita moschata (Rifu)cmocpeB703
Cp4.1LG04g08070Cucurbita moschata (Rifu)cmocpeB822
Cp4.1LG04g08070Wild cucumber (PI 183967)cpecpiB689
Cp4.1LG04g08070Cucumber (Chinese Long) v2cpecuB686
Cp4.1LG04g08070Bottle gourd (USVL1VR-Ls)cpelsiB536
Cp4.1LG04g08070Watermelon (Charleston Gray)cpewcgB588
Cp4.1LG04g08070Watermelon (Charleston Gray)cpewcgB611
Cp4.1LG04g08070Watermelon (Charleston Gray)cpewcgB624
Cp4.1LG04g08070Watermelon (97103) v1cpewmB656
Cp4.1LG04g08070Watermelon (97103) v1cpewmB663
Cp4.1LG04g08070Melon (DHL92) v3.5.1cpemeB644
Cp4.1LG04g08070Cucumber (Gy14) v2cgybcpeB237
Cp4.1LG04g08070Melon (DHL92) v3.6.1cpemedB746
Cp4.1LG04g08070Melon (DHL92) v3.6.1cpemedB772
Cp4.1LG04g08070Silver-seed gourdcarcpeB0296
Cp4.1LG04g08070Silver-seed gourdcarcpeB0529
Cp4.1LG04g08070Cucumber (Chinese Long) v3cpecucB0857
Cp4.1LG04g08070Wax gourdcpewgoB0841
Cp4.1LG04g08070Wax gourdcpewgoB0860