Carg20367 (gene) Silver-seed gourd | Cucurbit Genomics Database (CuGenDB)

Overview


Name	Carg20367
Type	gene
Organism	Cucurbita argyrosperma (Silver-seed gourd)
Description	Calcium-binding EF-hand family protein
Location	Cucurbita_argyrosperma_scaffold_178 : 342601 .. 343155 (+)

Genome Viewer

Gene Expression

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGCCGATGAACCCAAATCTCCTCGACCTGCAATACAGAGTCTCCAAGAAAAAGTTCCTCCGGGAGCCCTCCCGACTGTTCTCCTTCAAGGACCGGCAGAGCTCCGGCGTCCGGCGGGCTCTGCAACCGAGCACGGAGGAGATAAAGCAGATCTTCAACAAGTTCGACACGAACAGAGACGGGCGGATCTCAAAGCACGAATACAGGGGAGTTCTTAAGGCGCTGGGGAGAGGGAACTCGATGGAAGAGGTGCAGAAGATTTTCAGGGCGGTGGATACCGACGGCGATGGGTACATAAACTTGAATGAGTTCATGGAGGTTCACCGGAGTGGCGACGGAGTTCAGGCGAGAGATGTGGAATTTGCTTTTAAGACGTTTGATTTGAATGGGGATCGGAAGATTAGTGCAGAGGAAATTATGAGAGTGTTGAGGGGATTAGGGGAAAAGTGTAGCATTGAGGATTGCCGGCGGATGGTGAGGGCGGTGGACAGCGACGGCGACGGTATGGTGGACATTGATGAGTTCATGACTATGATGACTCGCTCTGTGATGTAA

mRNA sequence

ATGCCGATGAACCCAAATCTCCTCGACCTGCAATACAGAGTCTCCAAGAAAAAGTTCCTCCGGGAGCCCTCCCGACTGTTCTCCTTCAAGGACCGGCAGAGCTCCGGCGTCCGGCGGGCTCTGCAACCGAGCACGGAGGAGATAAAGCAGATCTTCAACAAGTTCGACACGAACAGAGACGGGCGGATCTCAAAGCACGAATACAGGGGAGTTCTTAAGGCGCTGGGGAGAGGGAACTCGATGGAAGAGGTGCAGAAGATTTTCAGGGCGGTGGATACCGACGGCGATGGGTACATAAACTTGAATGAGTTCATGGAGGTTCACCGGAGTGGCGACGGAGTTCAGGCGAGAGATGTGGAATTTGCTTTTAAGACGTTTGATTTGAATGGGGATCGGAAGATTAGTGCAGAGGAAATTATGAGAGTGTTGAGGGGATTAGGGGAAAAGTGTAGCATTGAGGATTGCCGGCGGATGGTGAGGGCGGTGGACAGCGACGGCGACGGTATGGTGGACATTGATGAGTTCATGACTATGATGACTCGCTCTGTGATGTAA

Coding sequence (CDS)

ATGCCGATGAACCCAAATCTCCTCGACCTGCAATACAGAGTCTCCAAGAAAAAGTTCCTCCGGGAGCCCTCCCGACTGTTCTCCTTCAAGGACCGGCAGAGCTCCGGCGTCCGGCGGGCTCTGCAACCGAGCACGGAGGAGATAAAGCAGATCTTCAACAAGTTCGACACGAACAGAGACGGGCGGATCTCAAAGCACGAATACAGGGGAGTTCTTAAGGCGCTGGGGAGAGGGAACTCGATGGAAGAGGTGCAGAAGATTTTCAGGGCGGTGGATACCGACGGCGATGGGTACATAAACTTGAATGAGTTCATGGAGGTTCACCGGAGTGGCGACGGAGTTCAGGCGAGAGATGTGGAATTTGCTTTTAAGACGTTTGATTTGAATGGGGATCGGAAGATTAGTGCAGAGGAAATTATGAGAGTGTTGAGGGGATTAGGGGAAAAGTGTAGCATTGAGGATTGCCGGCGGATGGTGAGGGCGGTGGACAGCGACGGCGACGGTATGGTGGACATTGATGAGTTCATGACTATGATGACTCGCTCTGTGATGTAA

Protein sequence

MPMNPNLLDLQYRVSKKKFLREPSRLFSFKDRQSSGVRRALQPSTEEIKQIFNKFDTNRDGRISKHEYRGVLKALGRGNSMEEVQKIFRAVDTDGDGYINLNEFMEVHRSGDGVQARDVEFAFKTFDLNGDRKISAEEIMRVLRGLGEKCSIEDCRRMVRAVDSDGDGMVDIDEFMTMMTRSVM

Homology

[Close]

BLAST of Carg20367 vs. NCBI nr
Match: XP_022927648.1 (calmodulin-like protein 1 [Cucurbita moschata])

HSP 1 Score: 237.3 bits (604), Expect = 4.2e-59
Identity = 183/184 (99.46%), Postives = 183/184 (99.46%), Query Frame = 0


Query: 1   MPMNPNLLDLQYRVSKKKFLREPSRLFSFKDRQSSGVRRALQPSTEEIKQIFNKFDTNRD 60
           MPMNPNLLDLQYRVSKKKFLREPSRLFSFKDRQSSGVRRALQPSTEEIKQIFNKFDTNRD
Sbjct: 1   MPMNPNLLDLQYRVSKKKFLREPSRLFSFKDRQSSGVRRALQPSTEEIKQIFNKFDTNRD 60

Query: 61  GRISKHEYRGVLKALGRGNXXXXXXXXXXXXXXXXXXXXXXXXXXXXHRSGDGVQAXXXX 120
           G ISKHEYRGVLKALGRGNXXXXXXXXXXXXXXXXXXXXXXXXXXXXHRSGDGVQAXXXX
Sbjct: 61  GLISKHEYRGVLKALGRGNXXXXXXXXXXXXXXXXXXXXXXXXXXXXHRSGDGVQAXXXX 120

Query: 121 XXXXXXXXXXXXXXXXXXXXXXLRGLGEKCSIEDCRRMVRAVDSDGDGMVDIDEFMTMMT 180
           XXXXXXXXXXXXXXXXXXXXXXLRGLGEKCSIEDCRRMVRAVDSDGDGMVDIDEFMTMMT
Sbjct: 121 XXXXXXXXXXXXXXXXXXXXXXLRGLGEKCSIEDCRRMVRAVDSDGDGMVDIDEFMTMMT 180

Query: 181 RSVM 185
           RSVM
Sbjct: 181 RSVM 184

[Close]

BLAST of Carg20367 vs. NCBI nr
Match: XP_023530136.1 (calmodulin-like protein 1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 235.7 bits (600), Expect = 1.2e-58
Identity = 178/184 (96.74%), Postives = 179/184 (97.28%), Query Frame = 0


Query: 1   MPMNPNLLDLQYRVSKKKFLREPSRLFSFKDRQSSGVRRALQPSTEEIKQIFNKFDTNRD 60
           MPMNPNLLDLQYRVSKKKFLREPSRLFSFKDRQSSGVRRALQPSTEEIKQIFNKFDTNRD
Sbjct: 1   MPMNPNLLDLQYRVSKKKFLREPSRLFSFKDRQSSGVRRALQPSTEEIKQIFNKFDTNRD 60

Query: 61  GRISKHEYRGVLKALGRGNXXXXXXXXXXXXXXXXXXXXXXXXXXXXHRSGDGVQAXXXX 120
           G ISKHEYRGVLKALGRGNXXXXXXXXXXXXXXXXXXXXXXXXXX  H+SGDGVQA XXX
Sbjct: 61  GLISKHEYRGVLKALGRGNXXXXXXXXXXXXXXXXXXXXXXXXXXEVHQSGDGVQARXXX 120

Query: 121 XXXXXXXXXXXXXXXXXXXXXXLRGLGEKCSIEDCRRMVRAVDSDGDGMVDIDEFMTMMT 180
           XXXXXXXXXXXXXXXXXXXXX LRGLGEKCSIEDCRRMVRAVDSDGDGMVDIDEFMTMMT
Sbjct: 121 XXXXXXXXXXXXXXXXXXXXXVLRGLGEKCSIEDCRRMVRAVDSDGDGMVDIDEFMTMMT 180

Query: 181 RSVM 185
           RSVM
Sbjct: 181 RSVM 184

[Close]

BLAST of Carg20367 vs. NCBI nr
Match: XP_022988967.1 (calmodulin-like protein 1 [Cucurbita maxima])

HSP 1 Score: 226.9 bits (577), Expect = 5.7e-56
Identity = 177/184 (96.20%), Postives = 177/184 (96.20%), Query Frame = 0


Query: 1   MPMNPNLLDLQYRVSKKKFLREPSRLFSFKDRQSSGVRRALQPSTEEIKQIFNKFDTNRD 60
           MPMNPNLLDLQYRVSKKKFLREPSRLFSFKDRQSSGVRRALQPSTEEIKQIFNKFDTNRD
Sbjct: 1   MPMNPNLLDLQYRVSKKKFLREPSRLFSFKDRQSSGVRRALQPSTEEIKQIFNKFDTNRD 60

Query: 61  GRISKHEYRGVLKALGRGNXXXXXXXXXXXXXXXXXXXXXXXXXXXXHRSGDGVQAXXXX 120
           GRISKHEYRGVLKALGRGNXXXXXXXXXXXXXXXXXXXXXXXXXXX HRSGD    XXXX
Sbjct: 61  GRISKHEYRGVLKALGRGNXXXXXXXXXXXXXXXXXXXXXXXXXXXVHRSGDXXXXXXXX 120

Query: 121 XXXXXXXXXXXXXXXXXXXXXXLRGLGEKCSIEDCRRMVRAVDSDGDGMVDIDEFMTMMT 180
           XXXXXXXXXXXXXXXXXXXXXX  GLGEKCSIEDCRRMVRAVDSDGDGMVDIDEFMTMMT
Sbjct: 121 XXXXXXXXXXXXXXXXXXXXXXXXGLGEKCSIEDCRRMVRAVDSDGDGMVDIDEFMTMMT 180

Query: 181 RSVM 185
           RSVM
Sbjct: 181 RSVM 184

[Close]

BLAST of Carg20367 vs. NCBI nr
Match: XP_004137114.2 (PREDICTED: calmodulin-like protein 1 [Cucumis sativus] >KGN43936.1 hypothetical protein Csa_7G073720 [Cucumis sativus])

HSP 1 Score: 208.4 bits (529), Expect = 2.1e-50
Identity = 166/182 (91.21%), Postives = 174/182 (95.60%), Query Frame = 0


Query: 1   MPMNPNLLDLQYRVSKKKFLREPSRLFSFKDRQSSGVRRALQPSTEEIKQIFNKFDTNRD 60
           MP+NPN L+LQY VSKKKFLREPSRLFSFKDRQSSG+RRALQPS+EEIKQIFNKFDTN+D
Sbjct: 1   MPINPNFLELQYNVSKKKFLREPSRLFSFKDRQSSGLRRALQPSSEEIKQIFNKFDTNKD 60

Query: 61  GRISKHEYRGVLKALGRGNXXXXXXXXXXXXXXXXXXXXXXXXXXXXHRSGDGVQAXXXX 120
           GRISKHEYRG+LKALGRGNXXXXXXXXXXXXXXXXXXXXXXXXXXXXHRS      XXXX
Sbjct: 61  GRISKHEYRGILKALGRGNXXXXXXXXXXXXXXXXXXXXXXXXXXXXHRSXXXXXXXXXX 120

Query: 121 XXXXXXXXXXXXXXXXXXXXXXLRGLGEKCSIEDCRRMVRAVDSDGDGMVDIDEFMTMMT 180
           XXXXXXXXXXXXXXXXXXXXXXL+GLGEKCSIEDCRRMVRAVDSDGDGMVDI+EFMTMMT
Sbjct: 121 XXXXXXXXXXXXXXXXXXXXXXLKGLGEKCSIEDCRRMVRAVDSDGDGMVDINEFMTMMT 180

Query: 181 RS 183
           RS
Sbjct: 181 RS 182

[Close]

BLAST of Carg20367 vs. NCBI nr
Match: XP_008454944.1 (PREDICTED: calmodulin-like protein 1 [Cucumis melo])

HSP 1 Score: 206.5 bits (524), Expect = 8.0e-50
Identity = 166/182 (91.21%), Postives = 172/182 (94.51%), Query Frame = 0


Query: 1   MPMNPNLLDLQYRVSKKKFLREPSRLFSFKDRQSSGVRRALQPSTEEIKQIFNKFDTNRD 60
           MP+NPN LDLQY VSKKKFLREPSRLFSFKDRQSSG+RRALQPS EEIKQIFNKFDTN+D
Sbjct: 1   MPINPNFLDLQYNVSKKKFLREPSRLFSFKDRQSSGLRRALQPSLEEIKQIFNKFDTNKD 60

Query: 61  GRISKHEYRGVLKALGRGNXXXXXXXXXXXXXXXXXXXXXXXXXXXXHRSGDGVQAXXXX 120
           GRISKHEYRG+LKALGRG XXXXXXXXXXXXXXXXXXXXXXXXXXXXHRS      XXXX
Sbjct: 61  GRISKHEYRGILKALGRGXXXXXXXXXXXXXXXXXXXXXXXXXXXXXHRSXXXXXXXXXX 120

Query: 121 XXXXXXXXXXXXXXXXXXXXXXLRGLGEKCSIEDCRRMVRAVDSDGDGMVDIDEFMTMMT 180
           XXXXXXXXXXXXXXXXXXXXXXL+GLGEKCSIEDCRRMVRAVDSDGDGMVDI+EFMTMMT
Sbjct: 121 XXXXXXXXXXXXXXXXXXXXXXLKGLGEKCSIEDCRRMVRAVDSDGDGMVDINEFMTMMT 180

Query: 181 RS 183
           RS
Sbjct: 181 RS 182

[Close]

BLAST of Carg20367 vs. TAIR10
Match: AT2G15680.1 (Calcium-binding EF-hand family protein)

HSP 1 Score: 113.2 bits (282), Expect = 1.7e-25
Identity = 82/182 (45.05%), Postives = 117/182 (64.29%), Query Frame = 0


Query: 4   NPNLLDLQYRVSKKKFLREPSRLFSFKDRQSSGVRRA-----LQPSTEEIKQIFNKFDTN 63
           N + L+LQY++SK K LR+PSR+FS +DRQSSG+         QPS  E++++F++FD +
Sbjct: 3   NVSFLELQYKLSKNKMLRKPSRMFS-RDRQSSGLSSPGPGGFSQPSVNEMRRVFSRFDLD 62

Query: 64  RDGRISKHEYRGVLKALGRGNXXXXXXXXXXXXXXXXXXXXXXXXXXXXHRSGDGVQAXX 123
           +DG+IS+ EY+ VL+ALG+      XXXXXXXXXXXXXXXXX       ++   G+++  
Sbjct: 63  KDGKISQTEYKVVLRALGQERAIEDXXXXXXXXXXXXXXXXXFREFIDAYKRSGGIRSSD 122

Query: 124 XXXXXXXXXXXXXXXXXXXXXXXXLRGLGEKCSIEDCRRMVRAVDSDGDGMVDIDEFMTM 181
                                   L  LGE+CS+EDC RMVRAVD+DGDG+V+++EF+ M
Sbjct: 123 IRNSFWTFDLNGDGKISAEEVMSVLWKLGERCSLEDCNRMVRAVDADGDGLVNMEEFIKM 182

[Close]

BLAST of Carg20367 vs. TAIR10
Match: AT1G18210.1 (Calcium-binding EF-hand family protein)

HSP 1 Score: 63.5 bits (153), Expect = 1.5e-10
Identity = 43/137 (31.39%), Postives = 56/137 (40.88%), Query Frame = 0


Query: 46  EEIKQIFNKFDTNRDGRISKHEYRGVLKALGRGNXXXXXXXXXXXXXXXXXXXXXXXXXX 105
           EE+K++F++FD+N DG+IS  E  GV KA+G                             
Sbjct: 22  EELKKVFDQFDSNGDGKISVLELGGVFKAMGTSYTETELNRVLEEVDTDRDGYINLDEFS 81

Query: 106 XXHRSGDGVQAXXXXXXXXXXXXXXXXXXXXXXXXXXLRGLGEKCSIEDCRRMVRAVDSD 165
              RS     A                          L  LG  CS+EDC RM+  VD+D
Sbjct: 82  TLCRSSS--SAAEIRDAFDLYDQDKNGLISASELHQVLNRLGMSCSVEDCTRMIGPVDAD 141

Query: 166 GDGMVDIDEFMTMMTRS 183
           GDG V+ +EF  MMT S
Sbjct: 142 GDGNVNFEEFQKMMTSS 156

[Close]

BLAST of Carg20367 vs. TAIR10
Match: AT1G66400.1 (calmodulin like 23)

HSP 1 Score: 60.5 bits (145), Expect = 1.3e-09
Identity = 87/150 (58.00%), Postives = 99/150 (66.00%), Query Frame = 0


Query: 34  SSGVRRALQPSTEEIKQIFNKFDTNRDGRISKHEYRGVLKALGRGNXXXXXXXXXXXXXX 93
           S  V R    S E+IK++F +FD N DG+IS  E + V+ AL    XXXXXXXXXXXXXX
Sbjct: 2   SKNVSRNCLGSMEDIKKVFQRFDKNNDGKISIDELKDVIGALSPNAXXXXXXXXXXXXXX 61

Query: 94  XXXXXXXXXXXXXXHRSGDGVQAXXXXXXXXXXXXXXXXXXXXXXXXXXL----RGLGEK 153
           XXXXXXXXXXXXXX         XXXXXXXXXXXXXXX           L    + LGEK
Sbjct: 62  XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXLDRNGRISANELHSVMKNLGEK 121

Query: 154 CSIEDCRRMVRAVDSDGDGMVDIDEFMTMM 180
           CSI+DC+RM+  VDSDGDG VD +EF  MM
Sbjct: 122 CSIQDCQRMINKVDSDGDGCVDFEEFKKMM 151

[Close]

BLAST of Carg20367 vs. TAIR10
Match: AT1G24620.1 (EF hand calcium-binding protein family)

HSP 1 Score: 55.5 bits (132), Expect = 4.1e-08
Identity = 37/144 (25.69%), Postives = 54/144 (37.50%), Query Frame = 0


Query: 39  RALQPSTEEIKQIFNKFDTNRDGRISKHEYRGVLKALGRGNXXXXXXXXXXXXXXXXXXX 98
           R+ +    E++ +F KFD N DG+IS  E   ++ +LG                      
Sbjct: 29  RSGKTEIRELEAVFKKFDVNGDGKISSKELGAIMTSLGHEVPEEELEKAITEIDRKGDGY 88

Query: 99  XXXXXXXXXHRSG--DGVQAXXXXXXXXXXXXXXXXXXXXXXXXXXLRGLGEKCSIEDCR 158
                    +  G                                 LR LG++CSI +CR
Sbjct: 89  INFEEFVELNTKGMDQNDVLENLKDAFSVYDIDGNGSISAEELHEVLRSLGDECSIAECR 148

Query: 159 RMVRAVDSDGDGMVDIDEFMTMMT 181
           +M+  VD DGDG +D +EF  MMT
Sbjct: 149 KMIGGVDKDGDGTIDFEEFKIMMT 172

[Close]

BLAST of Carg20367 vs. TAIR10
Match: AT1G73630.1 (EF hand calcium-binding protein family)

HSP 1 Score: 52.0 bits (123), Expect = 4.6e-07
Identity = 67/143 (46.85%), Postives = 83/143 (58.04%), Query Frame = 0


Query: 39  RALQPSTE-EIKQIFNKFDTNRDGRISKHEYRGVLKALGRGNXXXXXXXXXXXXXXXXXX 98
           ++  PST+ E+K++F+KFD N DG+IS  E   V K++  G XXXXXXXXXXXXXXXXXX
Sbjct: 11  KSTTPSTDMELKKVFDKFDANGDGKISVSELGNVFKSM--GXXXXXXXXXXXXXXXXXXX 70

Query: 99  XXXXXXXXXXHRSGDGVQAXXXXXXXXXXXXXXXXXXXXXXXXXXLRGLGEKCSIEDCRR 158
           XXXXXXXXXX                                   L  LG  CS+EDC R
Sbjct: 71  XXXXXXXXXXXXXXXXXXXVEIREAFDLYDQNKNGLISSSEIHKVLNRLGMTCSVEDCVR 130

Query: 159 MVRAVDSDGDGMVDIDEFMTMMT 181
           M+  VD+DGDG V+ +EF  MM+
Sbjct: 131 MIGHVDTDGDGNVNFEEFQKMMS 151

[Close]

BLAST of Carg20367 vs. Swiss-Prot
Match: sp|Q9ZQE6|CML30_ARATH (Calmodulin-like protein 30 OS=Arabidopsis thaliana OX=3702 GN=CML30 PE=2 SV=1)

HSP 1 Score: 113.2 bits (282), Expect = 3.0e-24
Identity = 82/182 (45.05%), Postives = 117/182 (64.29%), Query Frame = 0


Query: 4   NPNLLDLQYRVSKKKFLREPSRLFSFKDRQSSGVRRA-----LQPSTEEIKQIFNKFDTN 63
           N + L+LQY++SK K LR+PSR+FS +DRQSSG+         QPS  E++++F++FD +
Sbjct: 3   NVSFLELQYKLSKNKMLRKPSRMFS-RDRQSSGLSSPGPGGFSQPSVNEMRRVFSRFDLD 62

Query: 64  RDGRISKHEYRGVLKALGRGNXXXXXXXXXXXXXXXXXXXXXXXXXXXXHRSGDGVQAXX 123
           +DG+IS+ EY+ VL+ALG+      XXXXXXXXXXXXXXXXX       ++   G+++  
Sbjct: 63  KDGKISQTEYKVVLRALGQERAIEDXXXXXXXXXXXXXXXXXFREFIDAYKRSGGIRSSD 122

Query: 124 XXXXXXXXXXXXXXXXXXXXXXXXLRGLGEKCSIEDCRRMVRAVDSDGDGMVDIDEFMTM 181
                                   L  LGE+CS+EDC RMVRAVD+DGDG+V+++EF+ M
Sbjct: 123 IRNSFWTFDLNGDGKISAEEVMSVLWKLGERCSLEDCNRMVRAVDADGDGLVNMEEFIKM 182

[Close]

BLAST of Carg20367 vs. Swiss-Prot
Match: sp|Q9LE22|CML27_ARATH (Probable calcium-binding protein CML27 OS=Arabidopsis thaliana OX=3702 GN=CML27 PE=1 SV=1)

HSP 1 Score: 63.5 bits (153), Expect = 2.7e-09
Identity = 43/137 (31.39%), Postives = 56/137 (40.88%), Query Frame = 0


Query: 46  EEIKQIFNKFDTNRDGRISKHEYRGVLKALGRGNXXXXXXXXXXXXXXXXXXXXXXXXXX 105
           EE+K++F++FD+N DG+IS  E  GV KA+G                             
Sbjct: 22  EELKKVFDQFDSNGDGKISVLELGGVFKAMGTSYTETELNRVLEEVDTDRDGYINLDEFS 81

Query: 106 XXHRSGDGVQAXXXXXXXXXXXXXXXXXXXXXXXXXXLRGLGEKCSIEDCRRMVRAVDSD 165
              RS     A                          L  LG  CS+EDC RM+  VD+D
Sbjct: 82  TLCRSSS--SAAEIRDAFDLYDQDKNGLISASELHQVLNRLGMSCSVEDCTRMIGPVDAD 141

Query: 166 GDGMVDIDEFMTMMTRS 183
           GDG V+ +EF  MMT S
Sbjct: 142 GDGNVNFEEFQKMMTSS 156

[Close]

BLAST of Carg20367 vs. Swiss-Prot
Match: sp|Q9C8Y1|CML23_ARATH (Probable calcium-binding protein CML23 OS=Arabidopsis thaliana OX=3702 GN=CML23 PE=2 SV=1)

HSP 1 Score: 60.5 bits (145), Expect = 2.3e-08
Identity = 87/150 (58.00%), Postives = 99/150 (66.00%), Query Frame = 0


Query: 34  SSGVRRALQPSTEEIKQIFNKFDTNRDGRISKHEYRGVLKALGRGNXXXXXXXXXXXXXX 93
           S  V R    S E+IK++F +FD N DG+IS  E + V+ AL    XXXXXXXXXXXXXX
Sbjct: 2   SKNVSRNCLGSMEDIKKVFQRFDKNNDGKISIDELKDVIGALSPNAXXXXXXXXXXXXXX 61

Query: 94  XXXXXXXXXXXXXXHRSGDGVQAXXXXXXXXXXXXXXXXXXXXXXXXXXL----RGLGEK 153
           XXXXXXXXXXXXXX         XXXXXXXXXXXXXXX           L    + LGEK
Sbjct: 62  XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXLDRNGRISANELHSVMKNLGEK 121

Query: 154 CSIEDCRRMVRAVDSDGDGMVDIDEFMTMM 180
           CSI+DC+RM+  VDSDGDG VD +EF  MM
Sbjct: 122 CSIQDCQRMINKVDSDGDGCVDFEEFKKMM 151

[Close]

BLAST of Carg20367 vs. Swiss-Prot
Match: sp|Q0DJV6|CML18_ORYSJ (Probable calcium-binding protein CML18 OS=Oryza sativa subsp. japonica OX=39947 GN=CML18 PE=2 SV=1)

HSP 1 Score: 60.1 bits (144), Expect = 3.0e-08
Identity = 42/147 (28.57%), Postives = 56/147 (38.10%), Query Frame = 0


Query: 47  EIKQIFNKFDTNRDGRISKHEYRGVLKALGRGNXXXXXXXXXXXXXXXXXXXXXXXXXXX 106
           E++Q+F ++D N DG+IS  E   VL+ALG                              
Sbjct: 5   ELEQVFRRYDANGDGKISAEELASVLRALGAPLGPGEVRRMMDEMDSDRDGFVDLSEFAA 64

Query: 107 XH---------RSGD-----GVQAXXXXXXXXXXXXXXXXXXXXXXXXXXLRGLGEKCSI 166
            H         + GD                                   LR LG+KCS+
Sbjct: 65  FHCGPTPAHGGKGGDAKDQEAASEAELREAFRMYDADSNGKISARELHRVLRQLGDKCSV 124

Query: 167 EDCRRMVRAVDSDGDGMVDIDEFMTMM 180
            DC RM+R+VD+DGDG V+ DEF  MM
Sbjct: 125 ADCSRMIRSVDADGDGCVNFDEFKKMM 151

[Close]

BLAST of Carg20367 vs. Swiss-Prot
Match: sp|Q5ZCK5|CML16_ORYSJ (Probable calcium-binding protein CML16 OS=Oryza sativa subsp. japonica OX=39947 GN=CML16 PE=2 SV=1)

HSP 1 Score: 55.8 bits (133), Expect = 5.7e-07
Identity = 40/148 (27.03%), Postives = 53/148 (35.81%), Query Frame = 0


Query: 40  ALQPSTEEIKQIFNKFDTNRDGRISKHEYRGVLKAL------GRGNXXXXXXXXXXXXXX 99
           AL P+  EI+++F +FD + DGRIS  E   V +A+        G               
Sbjct: 20  ALAPADAEIERVFTRFDADGDGRISPSELAAVTRAIAPPPSESAGGREVAAMMNELDTDR 79

Query: 100 XXXXXXXXXXXXXXHRSGDGVQAXXXXXXXXXXXXXXXXXXXXXXXXXXLRGLGEKCSIE 159
                            GD                              L  +GE CS E
Sbjct: 80  DGFVDLGEFAAFHGRGRGDAEHEAELRAAFDVYDVDGDGRITAAELGKVLGRIGEGCSAE 139

Query: 160 DCRRMVRAVDSDGDGMVDIDEFMTMMTR 182
           +C RM+ +VD DGDG V  +EF  MM R
Sbjct: 140 ECERMIASVDVDGDGCVGFEEFKKMMCR 167

[Close]

BLAST of Carg20367 vs. TrEMBL
Match: tr|A0A0A0K804|A0A0A0K804_CUCSA (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_7G073720 PE=4 SV=1)

HSP 1 Score: 208.4 bits (529), Expect = 1.4e-50
Identity = 166/182 (91.21%), Postives = 174/182 (95.60%), Query Frame = 0


Query: 1   MPMNPNLLDLQYRVSKKKFLREPSRLFSFKDRQSSGVRRALQPSTEEIKQIFNKFDTNRD 60
           MP+NPN L+LQY VSKKKFLREPSRLFSFKDRQSSG+RRALQPS+EEIKQIFNKFDTN+D
Sbjct: 1   MPINPNFLELQYNVSKKKFLREPSRLFSFKDRQSSGLRRALQPSSEEIKQIFNKFDTNKD 60

Query: 61  GRISKHEYRGVLKALGRGNXXXXXXXXXXXXXXXXXXXXXXXXXXXXHRSGDGVQAXXXX 120
           GRISKHEYRG+LKALGRGNXXXXXXXXXXXXXXXXXXXXXXXXXXXXHRS      XXXX
Sbjct: 61  GRISKHEYRGILKALGRGNXXXXXXXXXXXXXXXXXXXXXXXXXXXXHRSXXXXXXXXXX 120

Query: 121 XXXXXXXXXXXXXXXXXXXXXXLRGLGEKCSIEDCRRMVRAVDSDGDGMVDIDEFMTMMT 180
           XXXXXXXXXXXXXXXXXXXXXXL+GLGEKCSIEDCRRMVRAVDSDGDGMVDI+EFMTMMT
Sbjct: 121 XXXXXXXXXXXXXXXXXXXXXXLKGLGEKCSIEDCRRMVRAVDSDGDGMVDINEFMTMMT 180

Query: 181 RS 183
           RS
Sbjct: 181 RS 182

[Close]

BLAST of Carg20367 vs. TrEMBL
Match: tr|A0A1S3C0X9|A0A1S3C0X9_CUCME (calmodulin-like protein 1 OS=Cucumis melo OX=3656 GN=LOC103495233 PE=4 SV=1)

HSP 1 Score: 206.5 bits (524), Expect = 5.3e-50
Identity = 166/182 (91.21%), Postives = 172/182 (94.51%), Query Frame = 0


Query: 1   MPMNPNLLDLQYRVSKKKFLREPSRLFSFKDRQSSGVRRALQPSTEEIKQIFNKFDTNRD 60
           MP+NPN LDLQY VSKKKFLREPSRLFSFKDRQSSG+RRALQPS EEIKQIFNKFDTN+D
Sbjct: 1   MPINPNFLDLQYNVSKKKFLREPSRLFSFKDRQSSGLRRALQPSLEEIKQIFNKFDTNKD 60

Query: 61  GRISKHEYRGVLKALGRGNXXXXXXXXXXXXXXXXXXXXXXXXXXXXHRSGDGVQAXXXX 120
           GRISKHEYRG+LKALGRG XXXXXXXXXXXXXXXXXXXXXXXXXXXXHRS      XXXX
Sbjct: 61  GRISKHEYRGILKALGRGXXXXXXXXXXXXXXXXXXXXXXXXXXXXXHRSXXXXXXXXXX 120

Query: 121 XXXXXXXXXXXXXXXXXXXXXXLRGLGEKCSIEDCRRMVRAVDSDGDGMVDIDEFMTMMT 180
           XXXXXXXXXXXXXXXXXXXXXXL+GLGEKCSIEDCRRMVRAVDSDGDGMVDI+EFMTMMT
Sbjct: 121 XXXXXXXXXXXXXXXXXXXXXXLKGLGEKCSIEDCRRMVRAVDSDGDGMVDINEFMTMMT 180

Query: 181 RS 183
           RS
Sbjct: 181 RS 182

[Close]

BLAST of Carg20367 vs. TrEMBL
Match: tr|A0A2P5CQP2|A0A2P5CQP2_9ROSA (Parvalbumin OS=Trema orientalis OX=63057 GN=TorRG33x02_276230 PE=4 SV=1)

HSP 1 Score: 154.1 bits (388), Expect = 3.1e-34
Identity = 78/180 (43.33%), Postives = 107/180 (59.44%), Query Frame = 0


Query: 4   NPNLLDLQYRVSKKKFLREPSRLFSFKDRQSSGVRRALQPSTEEIKQIFNKFDTNRDGRI 63
           N + LD QY +SK+K L+ PSRLFS +DRQSSG++ A  P+ +E+K +F+KFD+N+DG+I
Sbjct: 3   NISFLDFQYNISKRKLLQRPSRLFSSRDRQSSGLKPAFMPNPDEMKLVFDKFDSNKDGKI 62

Query: 64  SKHEYRGVLKALGRGNXXXXXXXXXXXXXXXXXXXXXXXXXXXXHRSGDGVQAXXXXXXX 123
           S+ EY+ VL+ALG+GN                            H+ G G++        
Sbjct: 63  SQQEYKAVLRALGKGNTAGEVTKIFQIVDLDGDGFINFKEFMEAHKKGGGIRTMDIQSAF 122

Query: 124 XXXXXXXXXXXXXXXXXXXLRGLGEKCSIEDCRRMVRAVDSDGDGMVDIDEFMTMMTRSV 183
                              LR LGE+CS++DCRRMVRAVD+DGDGMVD+DEFMTMMT ++
Sbjct: 123 HTFDANGDGKISPEEVLDVLRRLGEQCSLDDCRRMVRAVDTDGDGMVDMDEFMTMMTNNM 182

[Close]

BLAST of Carg20367 vs. TrEMBL
Match: tr|A0A2I4EEJ0|A0A2I4EEJ0_9ROSI (calmodulin-like protein 1 OS=Juglans regia OX=51240 GN=LOC108988889 PE=4 SV=1)

HSP 1 Score: 150.2 bits (378), Expect = 4.5e-33
Identity = 100/178 (56.18%), Postives = 129/178 (72.47%), Query Frame = 0


Query: 6   NLLDLQYRVSKKKFLREPSRLFSFKDRQSSGVRRALQPSTEEIKQIFNKFDTNRDGRISK 65
           + LD QY +SK KFLR+PSRLFS +DRQ+S ++ A QP+ +E+KQ+F+KFD+N+DG+IS+
Sbjct: 5   SFLDFQYNISKTKFLRKPSRLFSSRDRQNSSLKPAFQPNQDEMKQVFDKFDSNKDGKISQ 64

Query: 66  HEYRGVLKALGRGNXXXXXXXXXXXXXXXXXXXXXXXXXXXXHRSGDGVQAXXXXXXXXX 125
            EY+ +LKALG+GN                            H+   G++   XXXXXXX
Sbjct: 65  QEYKAILKALGKGNTIGEVSKIFQEVDLNGDGFIDFKEFMELHKKDGGIKTTDXXXXXXX 124

Query: 126 XXXXXXXXXXXXXXXXXLRGLGEKCSIEDCRRMVRAVDSDGDGMVDIDEFMTMMTRSV 184
           XXXXXXXXXXXXXXXXX   LGE+CS+EDCRRMVRAVD+DGDGMV++DEFM MMTR++
Sbjct: 125 XXXXXXXXXXXXXXXXXXGKLGERCSLEDCRRMVRAVDADGDGMVNLDEFMDMMTRNL 182

[Close]

BLAST of Carg20367 vs. TrEMBL
Match: tr|A0A2P5D716|A0A2P5D716_PARAD (Parvalbumin OS=Parasponia andersonii OX=3476 GN=PanWU01x14_091280 PE=4 SV=1)

HSP 1 Score: 147.9 bits (372), Expect = 2.2e-32
Identity = 76/180 (42.22%), Postives = 105/180 (58.33%), Query Frame = 0


Query: 4   NPNLLDLQYRVSKKKFLREPSRLFSFKDRQSSGVRRALQPSTEEIKQIFNKFDTNRDGRI 63
           N + LD QY +SK+K L+ PSRLFS +DRQSS +  A  P+ +E+K +F+KFD+N+DG+I
Sbjct: 3   NISFLDFQYNISKRKLLQGPSRLFSSRDRQSSRLTPAFMPNPDEMKLVFDKFDSNKDGKI 62

Query: 64  SKHEYRGVLKALGRGNXXXXXXXXXXXXXXXXXXXXXXXXXXXXHRSGDGVQAXXXXXXX 123
           S+ EY+ VL+ALG+GN                            H+ G G++        
Sbjct: 63  SQQEYKAVLRALGKGNTAGEVTKIFQIVDLDGDGFINFKEFMEAHKKGGGIRTMDIQSAF 122

Query: 124 XXXXXXXXXXXXXXXXXXXLRGLGEKCSIEDCRRMVRAVDSDGDGMVDIDEFMTMMTRSV 183
                              LR LGE+CS++DCRRMVRAVD+DGDG+VD+DEFMTMMT ++
Sbjct: 123 RTFDANDDGKISPEDVLDVLRRLGERCSLDDCRRMVRAVDTDGDGVVDMDEFMTMMTNNM 182

The following BLAST results are available for this feature:

BLAST of Carg20367 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022927648.1	4.2e-59	99.46	calmodulin-like protein 1 [Cucurbita moschata]	[more]
XP_023530136.1	1.2e-58	96.74	calmodulin-like protein 1 [Cucurbita pepo subsp. pepo]	[more]
XP_022988967.1	5.7e-56	96.20	calmodulin-like protein 1 [Cucurbita maxima]	[more]
XP_004137114.2	2.1e-50	91.21	PREDICTED: calmodulin-like protein 1 [Cucumis sativus] >KGN43936.1 hypothetical ...	[more]
XP_008454944.1	8.0e-50	91.21	PREDICTED: calmodulin-like protein 1 [Cucumis melo]	[more]

BLAST of Carg20367 vs. TAIR10


Match Name	E-value	Identity	Description
AT2G15680.1	1.7e-25	45.05	Calcium-binding EF-hand family protein	[more]
AT1G18210.1	1.5e-10	31.39	Calcium-binding EF-hand family protein	[more]
AT1G66400.1	1.3e-09	58.00	calmodulin like 23	[more]
AT1G24620.1	4.1e-08	25.69	EF hand calcium-binding protein family	[more]
AT1G73630.1	4.6e-07	46.85	EF hand calcium-binding protein family	[more]

BLAST of Carg20367 vs. Swiss-Prot


Match Name	E-value	Identity	Description
sp\|Q9ZQE6\|CML30_ARATH	3.0e-24	45.05	Calmodulin-like protein 30 OS=Arabidopsis thaliana OX=3702 GN=CML30 PE=2 SV=1	[more]
sp\|Q9LE22\|CML27_ARATH	2.7e-09	31.39	Probable calcium-binding protein CML27 OS=Arabidopsis thaliana OX=3702 GN=CML27 ...	[more]
sp\|Q9C8Y1\|CML23_ARATH	2.3e-08	58.00	Probable calcium-binding protein CML23 OS=Arabidopsis thaliana OX=3702 GN=CML23 ...	[more]
sp\|Q0DJV6\|CML18_ORYSJ	3.0e-08	28.57	Probable calcium-binding protein CML18 OS=Oryza sativa subsp. japonica OX=39947 ...	[more]
sp\|Q5ZCK5\|CML16_ORYSJ	5.7e-07	27.03	Probable calcium-binding protein CML16 OS=Oryza sativa subsp. japonica OX=39947 ...	[more]

BLAST of Carg20367 vs. TrEMBL


Match Name	E-value	Identity	Description
tr\|A0A0A0K804\|A0A0A0K804_CUCSA	1.4e-50	91.21	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_7G073720 PE=4 SV=1	[more]
tr\|A0A1S3C0X9\|A0A1S3C0X9_CUCME	5.3e-50	91.21	calmodulin-like protein 1 OS=Cucumis melo OX=3656 GN=LOC103495233 PE=4 SV=1	[more]
tr\|A0A2P5CQP2\|A0A2P5CQP2_9ROSA	3.1e-34	43.33	Parvalbumin OS=Trema orientalis OX=63057 GN=TorRG33x02_276230 PE=4 SV=1	[more]
tr\|A0A2I4EEJ0\|A0A2I4EEJ0_9ROSI	4.5e-33	56.18	calmodulin-like protein 1 OS=Juglans regia OX=51240 GN=LOC108988889 PE=4 SV=1	[more]
tr\|A0A2P5D716\|A0A2P5D716_PARAD	2.2e-32	42.22	Parvalbumin OS=Parasponia andersonii OX=3476 GN=PanWU01x14_091280 PE=4 SV=1	[more]

Annotated Terms

The following terms have been associated with this gene:

Vocabulary: **Molecular Function**
Term	Definition
GO:0005509	calcium ion binding

Vocabulary: **INTERPRO**
Term	Definition
IPR011992	EF-hand-dom_pair
IPR018247	EF_Hand_1_Ca_BS
IPR002048	EF_hand_dom

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0008150	biological_process
cellular_component	GO:0005575	cellular_component
molecular_function	GO:0005509	calcium ion binding

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Carg20367-RA	Carg20367-RA	mRNA

InterPro

Analysis Name: InterPro Annotations of silver-seed gourd
Date Performed: 2019-03-07


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR002048	EF-hand domain	SMART	SM00054	efh_1	coord: 47..75 e-value: 1.2E-6 score: 38.1 coord: 83..111 e-value: 2.3E-5 score: 33.8 coord: 154..182 e-value: 0.0063 score: 25.7 coord: 118..146 e-value: 0.0024 score: 27.1
IPR002048	EF-hand domain	PFAM	PF13499	EF-hand_7	coord: 119..179 e-value: 1.5E-11 score: 44.5 coord: 46..106 e-value: 2.4E-15 score: 56.6
IPR002048	EF-hand domain	PROSITE	PS50222	EF_HAND_2	coord: 43..78 score: 15.629
IPR002048	EF-hand domain	PROSITE	PS50222	EF_HAND_2	coord: 79..114 score: 14.179
IPR002048	EF-hand domain	PROSITE	PS50222	EF_HAND_2	coord: 118..149 score: 12.477
IPR002048	EF-hand domain	PROSITE	PS50222	EF_HAND_2	coord: 150..184 score: 11.194
IPR002048	EF-hand domain	CDD	cd00051	EFh	coord: 47..109 e-value: 5.06307E-18 score: 73.7361
None	No IPR available	GENE3D	G3DSA:1.10.238.10		coord: 8..110 e-value: 1.3E-23 score: 85.9
None	No IPR available	GENE3D	G3DSA:1.10.238.10		coord: 147..181 e-value: 2.5E-12 score: 48.5
None	No IPR available	GENE3D	G3DSA:1.10.238.10		coord: 111..146 e-value: 3.1E-6 score: 28.8
None	No IPR available	PANTHER	PTHR10891:SF620	CALMODULIN-LIKE PROTEIN 1	coord: 4..183
None	No IPR available	PANTHER	PTHR10891	EF-HAND CALCIUM-BINDING DOMAIN CONTAINING PROTEIN	coord: 4..183
IPR018247	EF-Hand 1, calcium-binding site	PROSITE	PS00018	EF_HAND_1	coord: 56..68
IPR018247	EF-Hand 1, calcium-binding site	PROSITE	PS00018	EF_HAND_1	coord: 127..139
IPR018247	EF-Hand 1, calcium-binding site	PROSITE	PS00018	EF_HAND_1	coord: 163..175
IPR018247	EF-Hand 1, calcium-binding site	PROSITE	PS00018	EF_HAND_1	coord: 92..104
IPR011992	EF-hand domain pair	SUPERFAMILY	SSF47473	EF-hand	coord: 45..181

Synteny

The following gene(s) are orthologous to this gene:


Gene	Orthologue	Organism	Block
Carg20367	Cla016007	Watermelon (97103) v1	carwmB0538
Carg20367	Csa7G073720	Cucumber (Chinese Long) v2	carcuB0571
Carg20367	MELO3C018445	Melon (DHL92) v3.5.1	carmeB0543
Carg20367	ClCG02G005880	Watermelon (Charleston Gray)	carwcgB0523
Carg20367	CSPI07G07690	Wild cucumber (PI 183967)	carcpiB0577
Carg20367	Cucsa.219910	Cucumber (Gy14) v1	carcgyB0618
Carg20367	CmoCh11G013580	Cucurbita moschata (Rifu)	carcmoB0728
Carg20367	Cp4.1LG04g08070	Cucurbita pepo (Zucchini)	carcpeB0764
Carg20367	CsGy7G007250	Cucumber (Gy14) v2	carcgybB0547
Carg20367	MELO3C018445.2	Melon (DHL92) v3.6.1	carmedB0548
Carg20367	CsaV3_7G008650	Cucumber (Chinese Long) v3	carcucB0578
Carg20367	Cla97C02G032860	Watermelon (97103) v2	carwmbB0564
Carg20367	Bhi05G000996	Wax gourd	carwgoB0650

The following gene(s) are paralogous to this gene:

None

The following block(s) are covering this gene:

None