Csa1G123470 (gene) Cucumber (Chinese Long) v2 | Cucurbit Genomics Database (CuGenDB)

Overview


Name	Csa1G123470
Type	gene
Organism	Cucumis. sativus (Cucumber (Chinese Long) v2)
Description	Cysteine proteinase inhibitor 4
Location	Chr1 : 9169805 .. 9170409 (+)

Genome Viewer

Gene Expression

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

AACTATATAAATACCAAACTCGTAATTAAAGTATTCATAAACAAATCATAGAATTAAAAGTATTACTACATACACAATATGGGTAGAGCAGAACCAATTAAGGATGTGAATGACTCAGAAGTGAAATTTGCTGCAGGGCAAGCAGTTAAAATCCATAACGATCAAAAACACGATACATTGGTTTTCATTCGTGTAGTGAATGGTCTGAAGCAGGAAGTTGATACTGCCAAGCTGTATTCTCTTCTGATAGAAGCTACAAATGGTGATGGAACTCATTGGGGATATATCGCTAAGATTAAGTTGTGGCCTAAGGCCCCAAGTCATTATGATCCATATTCCCATACCTTTGAGCTCATATCTTGGGAAGATCCCCTCAAATTCTGGAACTAAGTATTCCAATGTTATCTTTTTACATCTTTACATCTTTACATCTTTACATCATAATAATATGTCCTTCTCTATATATAGAGAGAGGGCATTATTGTCTTCCAACTACTATATGAAAATGGTATAGCCTTGATTAAATAGTGATACACATAATCTATTTCTATATAGTATATGTGTGTCCATATATATATGTTTAACTTTTTCTCTTCTCTCTCCCA

mRNA sequence

ATGGGTAGAGCAGAACCAATTAAGGATGTGAATGACTCAGAAGTGAAATTTGCTGCAGGGCAAGCAGTTAAAATCCATAACGATCAAAAACACGATACATTGGTTTTCATTCGTGTAGTGAATGGTCTGAAGCAGGAAGTTGATACTGCCAAGCTGTATTCTCTTCTGATAGAAGCTACAAATGGTGATGGAACTCATTGGGGATATATCGCTAAGATTAAGTTGTGGCCTAAGGCCCCAAGTCATTATGATCCATATTCCCATACCTTTGAGCTCATATCTTGGGAAGATCCCCTCAAATTCTGGAACTAA

Coding sequence (CDS)

ATGGGTAGAGCAGAACCAATTAAGGATGTGAATGACTCAGAAGTGAAATTTGCTGCAGGGCAAGCAGTTAAAATCCATAACGATCAAAAACACGATACATTGGTTTTCATTCGTGTAGTGAATGGTCTGAAGCAGGAAGTTGATACTGCCAAGCTGTATTCTCTTCTGATAGAAGCTACAAATGGTGATGGAACTCATTGGGGATATATCGCTAAGATTAAGTTGTGGCCTAAGGCCCCAAGTCATTATGATCCATATTCCCATACCTTTGAGCTCATATCTTGGGAAGATCCCCTCAAATTCTGGAACTAA

Protein sequence

MGRAEPIKDVNDSEVKFAAGQAVKIHNDQKHDTLVFIRVVNGLKQEVDTAKLYSLLIEATNGDGTHWGYIAKIKLWPKAPSHYDPYSHTFELISWEDPLKFWN*

Homology

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BLAST of Csa1G123470 vs. Swiss-Prot
Match: CYT4_ARATH (Cysteine proteinase inhibitor 4 OS=Arabidopsis thaliana GN=CYS4 PE=3 SV=2)

HSP 1 Score: 51.6 bits (122), Expect = 6.0e-06
Identity = 31/78 (39.74%), Postives = 42/78 (53.85%), Query Frame = 1


Query: 1   MGRAEPIKDVNDSEVKFAAGQAVKIHNDQKHDTLVFIRVVNGLKQEVDTAKLYSLLIEAT 60
           +G  +PIK+V+D +V   A  A++ HN +  + LVF++VV G  Q V   K Y L I A 
Sbjct: 29  LGSRKPIKNVSDPDVVAVAKYAIEEHNKESKEKLVFVKVVEGTTQVVSGTK-YDLKIAAK 88

Query: 61  NGDGTHWGYIAKI--KLW 77
           +G G    Y A +  KLW
Sbjct: 89  DGGGKIKNYEAVVVEKLW 105

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BLAST of Csa1G123470 vs. TrEMBL
Match: A0A0A0LVH4_CUCSA (Cystatin Hv-CPI6 OS=Cucumis sativus GN=Csa_1G123470 PE=4 SV=1)

HSP 1 Score: 220.3 bits (560), Expect = 1.1e-54
Identity = 103/103 (100.00%), Postives = 103/103 (100.00%), Query Frame = 1


Query: 1   MGRAEPIKDVNDSEVKFAAGQAVKIHNDQKHDTLVFIRVVNGLKQEVDTAKLYSLLIEAT 60
           MGRAEPIKDVNDSEVKFAAGQAVKIHNDQKHDTLVFIRVVNGLKQEVDTAKLYSLLIEAT
Sbjct: 1   MGRAEPIKDVNDSEVKFAAGQAVKIHNDQKHDTLVFIRVVNGLKQEVDTAKLYSLLIEAT 60

Query: 61  NGDGTHWGYIAKIKLWPKAPSHYDPYSHTFELISWEDPLKFWN 104
           NGDGTHWGYIAKIKLWPKAPSHYDPYSHTFELISWEDPLKFWN
Sbjct: 61  NGDGTHWGYIAKIKLWPKAPSHYDPYSHTFELISWEDPLKFWN 103

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BLAST of Csa1G123470 vs. TAIR10
Match: AT4G16500.1 (AT4G16500.1 Cystatin/monellin superfamily protein)

HSP 1 Score: 51.6 bits (122), Expect = 3.4e-07
Identity = 31/78 (39.74%), Postives = 42/78 (53.85%), Query Frame = 1


Query: 1   MGRAEPIKDVNDSEVKFAAGQAVKIHNDQKHDTLVFIRVVNGLKQEVDTAKLYSLLIEAT 60
           +G  +PIK+V+D +V   A  A++ HN +  + LVF++VV G  Q V   K Y L I A 
Sbjct: 29  LGSRKPIKNVSDPDVVAVAKYAIEEHNKESKEKLVFVKVVEGTTQVVSGTK-YDLKIAAK 88

Query: 61  NGDGTHWGYIAKI--KLW 77
           +G G    Y A +  KLW
Sbjct: 89  DGGGKIKNYEAVVVEKLW 105

[Close]

BLAST of Csa1G123470 vs. NCBI nr
Match: gi|700209736|gb|KGN64832.1| (Cystatin Hv-CPI6 [Cucumis sativus])

HSP 1 Score: 220.3 bits (560), Expect = 1.6e-54
Identity = 103/103 (100.00%), Postives = 103/103 (100.00%), Query Frame = 1


Query: 1   MGRAEPIKDVNDSEVKFAAGQAVKIHNDQKHDTLVFIRVVNGLKQEVDTAKLYSLLIEAT 60
           MGRAEPIKDVNDSEVKFAAGQAVKIHNDQKHDTLVFIRVVNGLKQEVDTAKLYSLLIEAT
Sbjct: 1   MGRAEPIKDVNDSEVKFAAGQAVKIHNDQKHDTLVFIRVVNGLKQEVDTAKLYSLLIEAT 60

Query: 61  NGDGTHWGYIAKIKLWPKAPSHYDPYSHTFELISWEDPLKFWN 104
           NGDGTHWGYIAKIKLWPKAPSHYDPYSHTFELISWEDPLKFWN
Sbjct: 61  NGDGTHWGYIAKIKLWPKAPSHYDPYSHTFELISWEDPLKFWN 103

[Close]

BLAST of Csa1G123470 vs. NCBI nr
Match: gi|727555227|ref|XP_010449778.1| (PREDICTED: cysteine proteinase inhibitor 4-like [Camelina sativa])

HSP 1 Score: 59.3 bits (142), Expect = 4.6e-06
Identity = 36/83 (43.37%), Postives = 47/83 (56.63%), Query Frame = 1


Query: 1   MGRAEPIKDVNDSEVKFAAGQAVKIHNDQKHDTLVFIRVVNGLKQEVDTAKLYSLLIEAT 60
           +G A+PIKDV+D  V   A  A++ HN Q  + LVF++VV G  Q V   K Y L I A 
Sbjct: 26  LGGAKPIKDVSDPNVVAIANYAIEEHNKQSKENLVFVKVVEGTTQVVSGTK-YDLKIAAK 85

Query: 61  NGDGTHWGYIAKI--KLWPKAPS 82
           NG G    Y A +  KLW ++ S
Sbjct: 86  NGGGQIKNYEAVVVEKLWLRSKS 107

The following BLAST results are available for this feature:

BLAST of Csa1G123470 vs. Swiss-Prot


Match Name	E-value	Identity	Description
CYT4_ARATH	6.0e-06	39.74	Cysteine proteinase inhibitor 4 OS=Arabidopsis thaliana GN=CYS4 PE=3 SV=2	[more]

BLAST of Csa1G123470 vs. TrEMBL


Match Name	E-value	Identity	Description
A0A0A0LVH4_CUCSA	1.1e-54	100.00	Cystatin Hv-CPI6 OS=Cucumis sativus GN=Csa_1G123470 PE=4 SV=1	[more]

BLAST of Csa1G123470 vs. TAIR10


Match Name	E-value	Identity	Description
AT4G16500.1	3.4e-07	39.74	Cystatin/monellin superfamily protein	[more]

BLAST of Csa1G123470 vs. NCBI nr


Match Name	E-value	Identity	Description
gi\|700209736\|gb\|KGN64832.1\|	1.6e-54	100.00	Cystatin Hv-CPI6 [Cucumis sativus]	[more]
gi\|727555227\|ref\|XP_010449778.1\|	4.6e-06	43.37	PREDICTED: cysteine proteinase inhibitor 4-like [Camelina sativa]	[more]

Annotated Terms

The following terms have been associated with this gene:

Vocabulary: **INTERPRO**
Term	Definition
IPR020381	Proteinase inhibitor I25, cystatin, conserved region

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0008150	biological_process
biological_process	GO:0010951	negative regulation of endopeptidase activity
cellular_component	GO:0005575	cellular_component
molecular_function	GO:0003674	molecular_function
molecular_function	GO:0004869	cysteine-type endopeptidase inhibitor activity

Unigene

This gene is associated with the following unigenes:


Unigene Name	Analysis Name	Sequence type in Unigene
CU086041	cucumber EST collection version 3.0	transcribed_cluster
CU107959	cucumber EST collection version 3.0	transcribed_cluster
CU131604	cucumber EST collection version 3.0	transcribed_cluster
CU132227	cucumber EST collection version 3.0	transcribed_cluster
CU153782	cucumber EST collection version 3.0	transcribed_cluster
CU178219	cucumber EST collection version 3.0	transcribed_cluster

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Csa1G123470.1	Csa1G123470.1	mRNA

The following transcribed_cluster feature(s) are associated with this gene:


Feature Name	Unique Name	Type
CU107959	CU107959	transcribed_cluster
CU131604	CU131604	transcribed_cluster
CU178219	CU178219	transcribed_cluster
CU132227	CU132227	transcribed_cluster
CU086041	CU086041	transcribed_cluster
CU153782	CU153782	transcribed_cluster

InterPro

Analysis Name: InterPro Annotations of cucumber (Chinese Long)
Date Performed: 2016-09-28


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR020381	Proteinase inhibitor I25, cystatin, conserved region	PFAM	PF16845	SQAPI	coord: 7..72 score: 2.8
None	No IPR available	GENE3D	G3DSA:3.10.450.10		coord: 2..98 score: 2.
None	No IPR available	unknown	SSF54403	Cystatin/monellin	coord: 4..66 score: 3.

Synteny

The following gene(s) are orthologous to this gene:


Gene	Orthologue	Organism	Block
Csa1G123470	MELO3C004914	Melon (DHL92) v3.5.1	cumeB015
Csa1G123470	MELO3C004914.2	Melon (DHL92) v3.6.1	cumedB010
Csa1G123470	CSPI01G13680	Wild cucumber (PI 183967)	cpicuB000
Csa1G123470	Cucsa.206990	Cucumber (Gy14) v1	cgycuB313
Csa1G123470	CsGy1G013700	Cucumber (Gy14) v2	cgybcuB001

The following gene(s) are paralogous to this gene:

None

The following block(s) are covering this gene:


Gene	Organism	Block
Csa1G123470	Cucurbita pepo (Zucchini)	cpecuB516
Csa1G123470	Cucurbita pepo (Zucchini)	cpecuB528
Csa1G123470	Bottle gourd (USVL1VR-Ls)	culsiB044
Csa1G123470	Cucumber (Gy14) v2	cgybcuB097
Csa1G123470	Silver-seed gourd	carcuB0185
Csa1G123470	Silver-seed gourd	carcuB0360
Csa1G123470	Silver-seed gourd	carcuB0472
Csa1G123470	Watermelon (97103) v2	cuwmbB034
Csa1G123470	Wax gourd	cuwgoB016
Csa1G123470	Wax gourd	cuwgoB049
Csa1G123470	Cucurbita maxima (Rimu)	cmacuB203
Csa1G123470	Cucurbita maxima (Rimu)	cmacuB431
Csa1G123470	Cucurbita moschata (Rifu)	cmocuB188
Csa1G123470	Cucurbita moschata (Rifu)	cmocuB421
Csa1G123470	Watermelon (Charleston Gray)	cuwcgB037
Csa1G123470	Watermelon (97103) v1	cuwmB010