CSPI01G13680 (gene) Wild cucumber (PI 183967) | Cucurbit Genomics Database (CuGenDB)

Overview


Name	CSPI01G13680
Type	gene
Organism	Cucumis sativus (Wild cucumber (PI 183967))
Description	Cystatin Hv-CPI6
Location	Chr1 : 9227740 .. 9228378 (+)

Genome Viewer

Gene Expression

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

GTATTCATAAACAAATCATAGAATTAAAAGTATTACTACATACACAATATGGGTAGAGCAGAACCAATTAAGGATGTGAATGACTCAGAAGTGAAATTTGCTGCAGGGCAAGCAGTTAAAATCCATAACGATCAAAAACACGATACATTGGTTTTCATTCGTGTAGTGAATGGTCTGAAGCAGGAAGTTGATACTGCCAAGCTGTATTCTCTTCTGATAGAAGCTACAAATGGTGATGGAACTCATTGGGGATATTTCGCTAAGATTAAGTTGTGGCCTAAGGCCCCAAGTCATTATGATCCATATTCCCATACCTTTGAGCTCATATCTTGGGAAGATCCCCTCAAATTCTGGAACTAAGTATTCCAATGTTATCTTTTTACATCTTTACATCTTTACATCTTTACATCATAATAATATGTCCTTCTATATATATATAGAGAGGGCATTATTGTCTTCCAACTACTATATGAAAATGGTATAGCCTTGATTAAATAGTGATACACATAATCTATTTCTATATAGTATATGTGTGTCCATATATATATGTTTAATTTTTTCTCTTCTCTCTCCCGTTTATGTTCTATGTATGTAAAGAGTTTAAATAAGACTAAACCATTAAATAGTTAATTTTACTTT

mRNA sequence

ATGGGTAGAGCAGAACCAATTAAGGATGTGAATGACTCAGAAGTGAAATTTGCTGCAGGGCAAGCAGTTAAAATCCATAACGATCAAAAACACGATACATTGGTTTTCATTCGTGTAGTGAATGGTCTGAAGCAGGAAGTTGATACTGCCAAGCTGTATTCTCTTCTGATAGAAGCTACAAATGGTGATGGAACTCATTGGGGATATTTCGCTAAGATTAAGTTGTGGCCTAAGGCCCCAAGTCATTATGATCCATATTCCCATACCTTTGAGCTCATATCTTGGGAAGATCCCCTCAAATTCTGGAACTAA

Coding sequence (CDS)

ATGGGTAGAGCAGAACCAATTAAGGATGTGAATGACTCAGAAGTGAAATTTGCTGCAGGGCAAGCAGTTAAAATCCATAACGATCAAAAACACGATACATTGGTTTTCATTCGTGTAGTGAATGGTCTGAAGCAGGAAGTTGATACTGCCAAGCTGTATTCTCTTCTGATAGAAGCTACAAATGGTGATGGAACTCATTGGGGATATTTCGCTAAGATTAAGTTGTGGCCTAAGGCCCCAAGTCATTATGATCCATATTCCCATACCTTTGAGCTCATATCTTGGGAAGATCCCCTCAAATTCTGGAACTAA

Homology

[Close]

BLAST of CSPI01G13680 vs. Swiss-Prot
Match: CYT4_ARATH (Cysteine proteinase inhibitor 4 OS=Arabidopsis thaliana GN=CYS4 PE=3 SV=2)

HSP 1 Score: 52.0 bits (123), Expect = 4.6e-06
Identity = 31/78 (39.74%), Postives = 44/78 (56.41%), Query Frame = 1


Query: 1   MGRAEPIKDVNDSEVKFAAGQAVKIHNDQKHDTLVFIRVVNGLKQEVDTAKLYSLLIEAT 60
           +G  +PIK+V+D +V   A  A++ HN +  + LVF++VV G  Q V   K Y L I A 
Sbjct: 29  LGSRKPIKNVSDPDVVAVAKYAIEEHNKESKEKLVFVKVVEGTTQVVSGTK-YDLKIAAK 88

Query: 61  NGDGTHWGYFAKI--KLW 77
           +G G    Y A +  KLW
Sbjct: 89  DGGGKIKNYEAVVVEKLW 105

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BLAST of CSPI01G13680 vs. TrEMBL
Match: A0A0A0LVH4_CUCSA (Cystatin Hv-CPI6 OS=Cucumis sativus GN=Csa_1G123470 PE=4 SV=1)

HSP 1 Score: 220.3 bits (560), Expect = 1.1e-54
Identity = 102/103 (99.03%), Postives = 102/103 (99.03%), Query Frame = 1


Query: 1   MGRAEPIKDVNDSEVKFAAGQAVKIHNDQKHDTLVFIRVVNGLKQEVDTAKLYSLLIEAT 60
           MGRAEPIKDVNDSEVKFAAGQAVKIHNDQKHDTLVFIRVVNGLKQEVDTAKLYSLLIEAT
Sbjct: 1   MGRAEPIKDVNDSEVKFAAGQAVKIHNDQKHDTLVFIRVVNGLKQEVDTAKLYSLLIEAT 60

Query: 61  NGDGTHWGYFAKIKLWPKAPSHYDPYSHTFELISWEDPLKFWN 104
           NGDGTHWGY AKIKLWPKAPSHYDPYSHTFELISWEDPLKFWN
Sbjct: 61  NGDGTHWGYIAKIKLWPKAPSHYDPYSHTFELISWEDPLKFWN 103

[Close]

BLAST of CSPI01G13680 vs. TAIR10
Match: AT4G16500.1 (AT4G16500.1 Cystatin/monellin superfamily protein)

HSP 1 Score: 52.0 bits (123), Expect = 2.6e-07
Identity = 31/78 (39.74%), Postives = 44/78 (56.41%), Query Frame = 1


Query: 1   MGRAEPIKDVNDSEVKFAAGQAVKIHNDQKHDTLVFIRVVNGLKQEVDTAKLYSLLIEAT 60
           +G  +PIK+V+D +V   A  A++ HN +  + LVF++VV G  Q V   K Y L I A 
Sbjct: 29  LGSRKPIKNVSDPDVVAVAKYAIEEHNKESKEKLVFVKVVEGTTQVVSGTK-YDLKIAAK 88

Query: 61  NGDGTHWGYFAKI--KLW 77
           +G G    Y A +  KLW
Sbjct: 89  DGGGKIKNYEAVVVEKLW 105

[Close]

BLAST of CSPI01G13680 vs. NCBI nr
Match: gi|700209736|gb|KGN64832.1| (Cystatin Hv-CPI6 [Cucumis sativus])

HSP 1 Score: 220.3 bits (560), Expect = 1.6e-54
Identity = 102/103 (99.03%), Postives = 102/103 (99.03%), Query Frame = 1


Query: 1   MGRAEPIKDVNDSEVKFAAGQAVKIHNDQKHDTLVFIRVVNGLKQEVDTAKLYSLLIEAT 60
           MGRAEPIKDVNDSEVKFAAGQAVKIHNDQKHDTLVFIRVVNGLKQEVDTAKLYSLLIEAT
Sbjct: 1   MGRAEPIKDVNDSEVKFAAGQAVKIHNDQKHDTLVFIRVVNGLKQEVDTAKLYSLLIEAT 60

Query: 61  NGDGTHWGYFAKIKLWPKAPSHYDPYSHTFELISWEDPLKFWN 104
           NGDGTHWGY AKIKLWPKAPSHYDPYSHTFELISWEDPLKFWN
Sbjct: 61  NGDGTHWGYIAKIKLWPKAPSHYDPYSHTFELISWEDPLKFWN 103

[Close]

BLAST of CSPI01G13680 vs. NCBI nr
Match: gi|727555227|ref|XP_010449778.1| (PREDICTED: cysteine proteinase inhibitor 4-like [Camelina sativa])

HSP 1 Score: 60.1 bits (144), Expect = 2.7e-06
Identity = 36/83 (43.37%), Postives = 47/83 (56.63%), Query Frame = 1


Query: 1   MGRAEPIKDVNDSEVKFAAGQAVKIHNDQKHDTLVFIRVVNGLKQEVDTAKLYSLLIEAT 60
           +G A+PIKDV+D  V   A  A++ HN Q  + LVF++VV G  Q V   K Y L I A 
Sbjct: 26  LGGAKPIKDVSDPNVVAIANYAIEEHNKQSKENLVFVKVVEGTTQVVSGTK-YDLKIAAK 85

Query: 61  NGDGTHWGYFAKI--KLWPKAPS 82
           NG G    Y A +  KLW ++ S
Sbjct: 86  NGGGQIKNYEAVVVEKLWLRSKS 107

The following BLAST results are available for this feature:

BLAST of CSPI01G13680 vs. Swiss-Prot


Match Name	E-value	Identity	Description
CYT4_ARATH	4.6e-06	39.74	Cysteine proteinase inhibitor 4 OS=Arabidopsis thaliana GN=CYS4 PE=3 SV=2	[more]

BLAST of CSPI01G13680 vs. TrEMBL


Match Name	E-value	Identity	Description
A0A0A0LVH4_CUCSA	1.1e-54	99.03	Cystatin Hv-CPI6 OS=Cucumis sativus GN=Csa_1G123470 PE=4 SV=1	[more]

BLAST of CSPI01G13680 vs. TAIR10


Match Name	E-value	Identity	Description
AT4G16500.1	2.6e-07	39.74	Cystatin/monellin superfamily protein	[more]

BLAST of CSPI01G13680 vs. NCBI nr


Match Name	E-value	Identity	Description
gi\|700209736\|gb\|KGN64832.1\|	1.6e-54	99.03	Cystatin Hv-CPI6 [Cucumis sativus]	[more]
gi\|727555227\|ref\|XP_010449778.1\|	2.7e-06	43.37	PREDICTED: cysteine proteinase inhibitor 4-like [Camelina sativa]	[more]

Annotated Terms

The following terms have been associated with this gene:

Vocabulary: **INTERPRO**
Term	Definition
IPR020381	Proteinase inhibitor I25, cystatin, conserved region

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0008150	biological_process
biological_process	GO:0010951	negative regulation of endopeptidase activity
cellular_component	GO:0005575	cellular_component
molecular_function	GO:0003674	molecular_function
molecular_function	GO:0004869	cysteine-type endopeptidase inhibitor activity

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CSPI01G13680.1	CSPI01G13680.1	mRNA

InterPro

Analysis Name: InterPro Annotations of cucumber (PI183967)
Date Performed: 2017-01-17


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR020381	Proteinase inhibitor I25, cystatin, conserved region	PFAM	PF16845	SQAPI	coord: 7..72 score: 3.2
None	No IPR available	GENE3D	G3DSA:3.10.450.10		coord: 2..98 score: 2.
None	No IPR available	unknown	SSF54403	Cystatin/monellin	coord: 4..66 score: 3.

Synteny

The following gene(s) are orthologous to this gene:


Gene	Orthologue	Organism	Block
CSPI01G13680	Csa1G123470	Cucumber (Chinese Long) v2	cpicuB000
CSPI01G13680	MELO3C004914	Melon (DHL92) v3.5.1	cpimeB011
CSPI01G13680	MELO3C004914.2	Melon (DHL92) v3.6.1	cpimedB011
CSPI01G13680	CsaV3_1G013900	Cucumber (Chinese Long) v3	cpicucB001
CSPI01G13680	Cucsa.206990	Cucumber (Gy14) v1	cgycpiB326
CSPI01G13680	CsGy1G013700	Cucumber (Gy14) v2	cgybcpiB001

The following gene(s) are paralogous to this gene:

None

The following block(s) are covering this gene:


Gene	Organism	Block
CSPI01G13680	Silver-seed gourd	carcpiB0192
CSPI01G13680	Silver-seed gourd	carcpiB0477
CSPI01G13680	Watermelon (97103) v2	cpiwmbB034
CSPI01G13680	Wax gourd	cpiwgoB017
CSPI01G13680	Wax gourd	cpiwgoB049
CSPI01G13680	Wild cucumber (PI 183967)	cpicpiB017
CSPI01G13680	Cucurbita maxima (Rimu)	cmacpiB205
CSPI01G13680	Cucurbita maxima (Rimu)	cmacpiB434
CSPI01G13680	Cucurbita moschata (Rifu)	cmocpiB192
CSPI01G13680	Cucurbita moschata (Rifu)	cmocpiB425
CSPI01G13680	Cucumber (Chinese Long) v2	cpicuB026
CSPI01G13680	Watermelon (Charleston Gray)	cpiwcgB007
CSPI01G13680	Watermelon (Charleston Gray)	cpiwcgB040
CSPI01G13680	Watermelon (97103) v1	cpiwmB007
CSPI01G13680	Watermelon (97103) v1	cpiwmB012
CSPI01G13680	Cucurbita pepo (Zucchini)	cpecpiB518
CSPI01G13680	Cucurbita pepo (Zucchini)	cpecpiB530
CSPI01G13680	Bottle gourd (USVL1VR-Ls)	cpilsiB046