MELO3C004914.2 (gene) Melon (DHL92) v3.6.1

Overview
NameMELO3C004914.2
Typegene
OrganismCucumis melo (Melon (DHL92) v3.6.1)
DescriptionCysteine proteinase inhibitor
Locationchr12 : 7023508 .. 7023819 (+)
Genome Viewer
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDSexon
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGAGTGGTGCCGAACCAATTAAGGATGTCAATGACTCAGAAGTAAAATTTGCTGCAGGGGAAGCAGTTAAAATCCATAACGATAAAAAGGACGACGATTTGGTTTTCATCCGCGTAGTGAATGGTGTGAAGCAGCAAGTTGATTATGGCAAGCAGTATTCTCtGCTCATAGAAGCTAGAAATGGTGATGGAACTCATTGGGGATATTGCGCTAAGATTAAGTTGCTGCGTAAGCCCGTAAGTCATCATGATCCATATCCGCATAGCTATGTGCTCATATCTTGGGAAGATCCCCTCAAATTCTACGACTAG

mRNA sequence

ATGAGTGGTGCCGAACCAATTAAGGATGTCAATGACTCAGAAGTAAAATTTGCTGCAGGGGAAGCAGTTAAAATCCATAACGATAAAAAGGACGACGATTTGGTTTTCATCCGCGTAGTGAATGGTGTGAAGCAGCAAGTTGATTATGGCAAGCAGTATTCTCtGCTCATAGAAGCTAGAAATGGTGATGGAACTCATTGGGGATATTGCGCTAAGATTAAGTTGCTGCGTAAGCCCGTAAGTCATCATGATCCATATCCGCATAGCTATGTGCTCATATCTTGGGAAGATCCCCTCAAATTCTACGACTAG

Coding sequence (CDS)

ATGAGTGGTGCCGAACCAATTAAGGATGTCAATGACTCAGAAGTAAAATTTGCTGCAGGGGAAGCAGTTAAAATCCATAACGATAAAAAGGACGACGATTTGGTTTTCATCCGCGTAGTGAATGGTGTGAAGCAGCAAGTTGATTATGGCAAGCAGTATTCTCtGCTCATAGAAGCTAGAAATGGTGATGGAACTCATTGGGGATATTGCGCTAAGATTAAGTTGCTGCGTAAGCCCGTAAGTCATCATGATCCATATCCGCATAGCTATGTGCTCATATCTTGGGAAGATCCCCTCAAATTCTACGACTAG

Protein sequence

MSGAEPIKDVNDSEVKFAAGEAVKIHNDKKDDDLVFIRVVNGVKQQVDYGKQYSLLIEARNGDGTHWGYCAKIKLLRKPVSHHDPYPHSYVLISWEDPLKFYD
Homology
[Close]
BLAST of MELO3C004914.2 vs. NCBI nr
Match: KGN64832.1 (Cystatin Hv-CPI6 [Cucumis sativus])

HSP 1 Score: 165.2 bits (417), Expect = 1.1e-37
Identity = 79/103 (76.70%), Postives = 88/103 (85.44%), Query Frame = 0 

Query: 1   MSGAEPIKDVNDSEVKFAAGEAVKIHNDKKDDDLVFIRVVNGVKQQVDYGKQYSLLIEAR 60
           M  AEPIKDVNDSEVKFAAG+AVKIHND+K D LVFIRVVNG+KQ+VD  K YSLLIEA 
Sbjct: 1   MGRAEPIKDVNDSEVKFAAGQAVKIHNDQKHDTLVFIRVVNGLKQEVDTAKLYSLLIEAT 60

Query: 61  NGDGTHWGYCAKIKLLRKPVSHHDPYPHSYVLISWEDPLKFYD 104
           NGDGTHWGY AKIKL  K  SH+DPY H++ LISWEDPLKF++
Sbjct: 61  NGDGTHWGYIAKIKLWPKAPSHYDPYSHTFELISWEDPLKFWN 103

[Close]
BLAST of MELO3C004914.2 vs. NCBI nr
Match: XP_006414340.1 (cysteine proteinase inhibitor 4 [Eutrema salsugineum] >ESQ55793.1 hypothetical protein EUTSA_v10026611mg [Eutrema salsugineum])

HSP 1 Score: 57.0 bits (136), Expect = 4.4e-05
Identity = 32/73 (43.84%), Postives = 46/73 (63.01%), Query Frame = 0 

Query: 1  MSGAEPIKDVNDSEVKFAAGEAVKIHNDKKDDDLVFIRVVNGVKQQVDYGKQYSLLIEAR 60
          + G +PIKDV+D  V+  A  A++ HN +   +LVF +VV G K+QV  GK+Y L+I A+
Sbjct: 23 LGGWKPIKDVSDPNVEAIAKYAIEEHNKQSKANLVFEKVVRG-KEQVVSGKKYDLIIAAK 82

Query: 61 NGDGTHWGYCAKI 74
          NG G    Y A +
Sbjct: 83 NGGGATKNYEAVV 94

[Close]
BLAST of MELO3C004914.2 vs. NCBI nr
Match: XP_010449778.1 (PREDICTED: cysteine proteinase inhibitor 4-like [Camelina sativa])

HSP 1 Score: 56.6 bits (135), Expect = 5.7e-05
Identity = 31/73 (42.47%), Postives = 44/73 (60.27%), Query Frame = 0 

Query: 1  MSGAEPIKDVNDSEVKFAAGEAVKIHNDKKDDDLVFIRVVNGVKQQVDYGKQYSLLIEAR 60
          + GA+PIKDV+D  V   A  A++ HN +  ++LVF++VV G  Q V  G +Y L I A+
Sbjct: 26 LGGAKPIKDVSDPNVVAIANYAIEEHNKQSKENLVFVKVVEGTTQVVS-GTKYDLKIAAK 85

Query: 61 NGDGTHWGYCAKI 74
          NG G    Y A +
Sbjct: 86 NGGGQIKNYEAVV 97

[Close]
BLAST of MELO3C004914.2 vs. NCBI nr
Match: XP_010434812.1 (PREDICTED: cysteine proteinase inhibitor 4-like [Camelina sativa])

HSP 1 Score: 54.3 bits (129), Expect = 2.8e-04
Identity = 29/73 (39.73%), Postives = 44/73 (60.27%), Query Frame = 0 

Query: 1  MSGAEPIKDVNDSEVKFAAGEAVKIHNDKKDDDLVFIRVVNGVKQQVDYGKQYSLLIEAR 60
          + GA+PIKD++D  V   A  A++ HN +  ++LVF++VV G  Q V  G +Y + I A+
Sbjct: 26 LGGAKPIKDLSDPNVVAVAKYAIEEHNKQSKENLVFVKVVEGTTQVVS-GTKYDMKIAAK 85

Query: 61 NGDGTHWGYCAKI 74
          NG G    Y A +
Sbjct: 86 NGGGQIKNYEAVV 97

[Close]
BLAST of MELO3C004914.2 vs. NCBI nr
Match: XP_013591334.1 (PREDICTED: cysteine proteinase inhibitor 4-like [Brassica oleracea var. oleracea] >XP_013691433.1 cysteine proteinase inhibitor 4 [Brassica napus] >CDY25483.1 BnaC09g39980D [Brassica napus])

HSP 1 Score: 53.9 bits (128), Expect = 3.7e-04
Identity = 33/83 (39.76%), Postives = 48/83 (57.83%), Query Frame = 0 

Query: 1   MSGAEPIKDVNDSEVKFAAGEAVKIHNDKKDDDLVFIRVVNGVKQQVDYGKQYSLLIEAR 60
           + G +PIKD++D  ++  A  AV  HN +   +LVF+++V G K+QV  GK YSL I A+
Sbjct: 22  LGGWKPIKDLSDPNIQAVAKYAVDEHNKQITGNLVFVKIVEG-KEQVVSGKTYSLTIAAK 81

Query: 61  NGDGTHWGYCAKIKLLRKPVSHH 84
           NG      Y A +  +    SHH
Sbjct: 82  NGGAGTKNYEAVV--VETMRSHH 101

[Close]
BLAST of MELO3C004914.2 vs. TAIR10
Match: AT4G16500.1 (Cystatin/monellin superfamily protein)

HSP 1 Score: 48.1 bits (113), Expect = 3.7e-06
Identity = 27/73 (36.99%), Postives = 42/73 (57.53%), Query Frame = 0 

Query: 1   MSGAEPIKDVNDSEVKFAAGEAVKIHNDKKDDDLVFIRVVNGVKQQVDYGKQYSLLIEAR 60
           +   +PIK+V+D +V   A  A++ HN +  + LVF++VV G  Q V  G +Y L I A+
Sbjct: 29  LGSRKPIKNVSDPDVVAVAKYAIEEHNKESKEKLVFVKVVEGTTQVVS-GTKYDLKIAAK 88

Query: 61  NGDGTHWGYCAKI 74
           +G G    Y A +
Sbjct: 89  DGGGKIKNYEAVV 100

[Close]
BLAST of MELO3C004914.2 vs. Swiss-Prot
Match: sp|Q84WT8|CYT4_ARATH (Cysteine proteinase inhibitor 4 OS=Arabidopsis thaliana OX=3702 GN=CYS4 PE=3 SV=2)

HSP 1 Score: 48.1 bits (113), Expect = 6.7e-05
Identity = 27/73 (36.99%), Postives = 42/73 (57.53%), Query Frame = 0 

Query: 1   MSGAEPIKDVNDSEVKFAAGEAVKIHNDKKDDDLVFIRVVNGVKQQVDYGKQYSLLIEAR 60
           +   +PIK+V+D +V   A  A++ HN +  + LVF++VV G  Q V  G +Y L I A+
Sbjct: 29  LGSRKPIKNVSDPDVVAVAKYAIEEHNKESKEKLVFVKVVEGTTQVVS-GTKYDLKIAAK 88

Query: 61  NGDGTHWGYCAKI 74
           +G G    Y A +
Sbjct: 89  DGGGKIKNYEAVV 100

[Close]
BLAST of MELO3C004914.2 vs. TrEMBL
Match: tr|A0A0A0LVH4|A0A0A0LVH4_CUCSA (Cystatin Hv-CPI6 OS=Cucumis sativus OX=3659 GN=Csa_1G123470 PE=4 SV=1)

HSP 1 Score: 165.2 bits (417), Expect = 7.6e-38
Identity = 79/103 (76.70%), Postives = 88/103 (85.44%), Query Frame = 0 

Query: 1   MSGAEPIKDVNDSEVKFAAGEAVKIHNDKKDDDLVFIRVVNGVKQQVDYGKQYSLLIEAR 60
           M  AEPIKDVNDSEVKFAAG+AVKIHND+K D LVFIRVVNG+KQ+VD  K YSLLIEA 
Sbjct: 1   MGRAEPIKDVNDSEVKFAAGQAVKIHNDQKHDTLVFIRVVNGLKQEVDTAKLYSLLIEAT 60

Query: 61  NGDGTHWGYCAKIKLLRKPVSHHDPYPHSYVLISWEDPLKFYD 104
           NGDGTHWGY AKIKL  K  SH+DPY H++ LISWEDPLKF++
Sbjct: 61  NGDGTHWGYIAKIKLWPKAPSHYDPYSHTFELISWEDPLKFWN 103

[Close]
BLAST of MELO3C004914.2 vs. TrEMBL
Match: tr|V4MRH3|V4MRH3_EUTSA (Cysteine proteinase inhibitor OS=Eutrema salsugineum OX=72664 GN=EUTSA_v10026611mg PE=3 SV=1)

HSP 1 Score: 57.0 bits (136), Expect = 2.9e-05
Identity = 32/73 (43.84%), Postives = 46/73 (63.01%), Query Frame = 0 

Query: 1  MSGAEPIKDVNDSEVKFAAGEAVKIHNDKKDDDLVFIRVVNGVKQQVDYGKQYSLLIEAR 60
          + G +PIKDV+D  V+  A  A++ HN +   +LVF +VV G K+QV  GK+Y L+I A+
Sbjct: 23 LGGWKPIKDVSDPNVEAIAKYAIEEHNKQSKANLVFEKVVRG-KEQVVSGKKYDLIIAAK 82

Query: 61 NGDGTHWGYCAKI 74
          NG G    Y A +
Sbjct: 83 NGGGATKNYEAVV 94

[Close]
BLAST of MELO3C004914.2 vs. TrEMBL
Match: tr|A0A0D3A7L6|A0A0D3A7L6_BRAOL (Cysteine proteinase inhibitor OS=Brassica oleracea var. oleracea OX=109376 GN=106299867 PE=3 SV=1)

HSP 1 Score: 53.9 bits (128), Expect = 2.5e-04
Identity = 33/83 (39.76%), Postives = 48/83 (57.83%), Query Frame = 0 

Query: 1   MSGAEPIKDVNDSEVKFAAGEAVKIHNDKKDDDLVFIRVVNGVKQQVDYGKQYSLLIEAR 60
           + G +PIKD++D  ++  A  AV  HN +   +LVF+++V G K+QV  GK YSL I A+
Sbjct: 22  LGGWKPIKDLSDPNIQAVAKYAVDEHNKQITGNLVFVKIVEG-KEQVVSGKTYSLTIAAK 81

Query: 61  NGDGTHWGYCAKIKLLRKPVSHH 84
           NG      Y A +  +    SHH
Sbjct: 82  NGGAGTKNYEAVV--VETMRSHH 101

[Close]
BLAST of MELO3C004914.2 vs. TrEMBL
Match: tr|A0A078GLJ0|A0A078GLJ0_BRANA (Cysteine proteinase inhibitor OS=Brassica napus OX=3708 GN=BnaC09g39980D PE=3 SV=1)

HSP 1 Score: 53.9 bits (128), Expect = 2.5e-04
Identity = 33/83 (39.76%), Postives = 48/83 (57.83%), Query Frame = 0 

Query: 1   MSGAEPIKDVNDSEVKFAAGEAVKIHNDKKDDDLVFIRVVNGVKQQVDYGKQYSLLIEAR 60
           + G +PIKD++D  ++  A  AV  HN +   +LVF+++V G K+QV  GK YSL I A+
Sbjct: 22  LGGWKPIKDLSDPNIQAVAKYAVDEHNKQITGNLVFVKIVEG-KEQVVSGKTYSLTIAAK 81

Query: 61  NGDGTHWGYCAKIKLLRKPVSHH 84
           NG      Y A +  +    SHH
Sbjct: 82  NGGAGTKNYEAVV--VETMRSHH 101

[Close]
BLAST of MELO3C004914.2 vs. TrEMBL
Match: tr|M4FBL2|M4FBL2_BRARP (Cysteine proteinase inhibitor OS=Brassica rapa subsp. pekinensis OX=51351 PE=3 SV=1)

HSP 1 Score: 52.8 bits (125), Expect = 5.5e-04
Identity = 32/83 (38.55%), Postives = 48/83 (57.83%), Query Frame = 0 

Query: 1   MSGAEPIKDVNDSEVKFAAGEAVKIHNDKKDDDLVFIRVVNGVKQQVDYGKQYSLLIEAR 60
           + G  PIKD++D  ++  A  AV+ HN +   +LVF++++ G K+QV  GK YSL I A+
Sbjct: 22  LGGWTPIKDLSDPNIQAVAKYAVEEHNKQITGNLVFVKILKG-KEQVVAGKTYSLTIAAK 81

Query: 61  NGDGTHWGYCAKIKLLRKPVSHH 84
           NG      Y A +  +    SHH
Sbjct: 82  NGGAGTKNYEAVV--VETMRSHH 101

The following BLAST results are available for this feature:
BLAST of MELO3C004914.2 vs. NCBI nr
Match NameE-valueIdentityDescription
KGN64832.11.1e-3776.70Cystatin Hv-CPI6 [Cucumis sativus][more]
XP_006414340.14.4e-0543.84cysteine proteinase inhibitor 4 [Eutrema salsugineum] >ESQ55793.1 hypothetical p... [more]
XP_010449778.15.7e-0542.47PREDICTED: cysteine proteinase inhibitor 4-like [Camelina sativa][more]
XP_010434812.12.8e-0439.73PREDICTED: cysteine proteinase inhibitor 4-like [Camelina sativa][more]
XP_013591334.13.7e-0439.76PREDICTED: cysteine proteinase inhibitor 4-like [Brassica oleracea var. oleracea... [more]
BLAST of MELO3C004914.2 vs. TAIR10
Match NameE-valueIdentityDescription
AT4G16500.13.7e-0636.99Cystatin/monellin superfamily protein[more]
BLAST of MELO3C004914.2 vs. Swiss-Prot
Match NameE-valueIdentityDescription
sp|Q84WT8|CYT4_ARATH6.7e-0536.99Cysteine proteinase inhibitor 4 OS=Arabidopsis thaliana OX=3702 GN=CYS4 PE=3 SV=... [more]
BLAST of MELO3C004914.2 vs. TrEMBL
Match NameE-valueIdentityDescription
tr|A0A0A0LVH4|A0A0A0LVH4_CUCSA7.6e-3876.70Cystatin Hv-CPI6 OS=Cucumis sativus OX=3659 GN=Csa_1G123470 PE=4 SV=1[more]
tr|V4MRH3|V4MRH3_EUTSA2.9e-0543.84Cysteine proteinase inhibitor OS=Eutrema salsugineum OX=72664 GN=EUTSA_v10026611... [more]
tr|A0A0D3A7L6|A0A0D3A7L6_BRAOL2.5e-0439.76Cysteine proteinase inhibitor OS=Brassica oleracea var. oleracea OX=109376 GN=10... [more]
tr|A0A078GLJ0|A0A078GLJ0_BRANA2.5e-0439.76Cysteine proteinase inhibitor OS=Brassica napus OX=3708 GN=BnaC09g39980D PE=3 SV... [more]
tr|M4FBL2|M4FBL2_BRARP5.5e-0438.55Cysteine proteinase inhibitor OS=Brassica rapa subsp. pekinensis OX=51351 PE=3 S... [more]
Annotated Terms
The following terms have been associated with this gene:
Vocabulary: Molecular Function
TermDefinition
GO:0004869cysteine-type endopeptidase inhibitor activity
Vocabulary: INTERPRO
TermDefinition
IPR000010Cystatin_dom
GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0010951 negative regulation of endopeptidase activity
biological_process GO:0010466 negative regulation of peptidase activity
biological_process GO:0008150 biological_process
cellular_component GO:0005575 cellular_component
molecular_function GO:0004869 cysteine-type endopeptidase inhibitor activity
molecular_function GO:0030414 peptidase inhibitor activity
molecular_function GO:0003674 molecular_function
Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
MELO3C004914.2.1MELO3C004914.2.1mRNA


InterPro
Analysis Name: InterPro Annotations of melon v3.6.1
Date Performed: 2018-09-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000010Cystatin domainPFAMPF16845SQAPIcoord: 7..72
e-value: 5.0E-13
score: 49.1
NoneNo IPR availableGENE3DG3DSA:3.10.450.10coord: 1..97
e-value: 1.4E-13
score: 52.6
NoneNo IPR availableSUPERFAMILYSSF54403Cystatin/monellincoord: 3..66

Synteny
The following gene(s) are orthologous to this gene:
GeneOrthologueOrganismBlock
MELO3C004914.2Csa1G123470Cucumber (Chinese Long) v2cumedB010
MELO3C004914.2CSPI01G13680Wild cucumber (PI 183967)cpimedB011
MELO3C004914.2Cucsa.206990Cucumber (Gy14) v1cgymedB370
MELO3C004914.2CsGy1G013700Cucumber (Gy14) v2cgybmedB009
MELO3C004914.2CsaV3_1G013900Cucumber (Chinese Long) v3cucmedB009
The following gene(s) are paralogous to this gene:

None

The following block(s) are covering this gene:
GeneOrganismBlock
MELO3C004914.2Watermelon (97103) v2medwmbB142
MELO3C004914.2Wax gourdmedwgoB176
MELO3C004914.2Cucurbita maxima (Rimu)cmamedB215
MELO3C004914.2Cucurbita maxima (Rimu)cmamedB451
MELO3C004914.2Cucurbita moschata (Rifu)cmomedB203
MELO3C004914.2Cucurbita moschata (Rifu)cmomedB439
MELO3C004914.2Cucurbita pepo (Zucchini)cpemedB563
MELO3C004914.2Cucurbita pepo (Zucchini)cpemedB583
MELO3C004914.2Bottle gourd (USVL1VR-Ls)lsimedB255
MELO3C004914.2Watermelon (Charleston Gray)medwcgB141
MELO3C004914.2Watermelon (97103) v1medwmB143
MELO3C004914.2Silver-seed gourdcarmedB0452