Cp4.1LG01g03820 (gene) Cucurbita pepo (Zucchini)

Overview
NameCp4.1LG01g03820
Typegene
OrganismCucurbita pepo (Cucurbita pepo (Zucchini))
DescriptionPlant invertase/pectin methylesterase inhibitor
LocationCp4.1LG01 : 1643760 .. 1644347 (+)
Genome Viewer
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCTAAATCCGCCTTCCTTCTCCTACTCCTCTCTATCTCCATCCTATTCGCCACCTCCAGCGCTGCCGACTTCGGCGGAGCTTCCAATTTCATCAAATCGAGATGCAATTTCACAACGTACCCTGACCTATGCGTTCAATCCCTCACAAATTTCGCCTCAAGAATCCAACAAAACCCTCGCCAATTAGTCCAAACCGCTCTAATGGTGAGCCTTTCTCACGCTCGATCCGCCCGATCCTTCGTCTGGAAACTGACGAAGTTCACCGGATTGAAGCCGCGAGAGTACGCGGCGTTGAGAGACTGTCTAGAGGAGGTCGGCGACACGGTGGAACGGCTGAGCAAATCGGTGGAAGAACTGAAGCTGGTTAGGAGATCGAAGAGGAAGGATTTCGAATGGCACATTAGCAACGTGGAGACTTGGGTGAGCGCGGCGATGACGGACGGGAACACTTGCTCCGATGGATTTGCAGGGGCGGTGCTGAATGGAAGAATTAAATCATCGATTAGGCGTCGCATTGTGGATTTAACTCGATTCATAAGTAATTCCTTGGCTCTGATTAACAACTATGCTCAAAATCAAAGCTAG

mRNA sequence

ATGGCTAAATCCGCCTTCCTTCTCCTACTCCTCTCTATCTCCATCCTATTCGCCACCTCCAGCGCTGCCGACTTCGGCGGAGCTTCCAATTTCATCAAATCGAGATGCAATTTCACAACGTACCCTGACCTATGCGTTCAATCCCTCACAAATTTCGCCTCAAGAATCCAACAAAACCCTCGCCAATTAGTCCAAACCGCTCTAATGGTGAGCCTTTCTCACGCTCGATCCGCCCGATCCTTCGTCTGGAAACTGACGAAGTTCACCGGATTGAAGCCGCGAGAGTACGCGGCGTTGAGAGACTGTCTAGAGGAGGTCGGCGACACGGTGGAACGGCTGAGCAAATCGGTGGAAGAACTGAAGCTGGTTAGGAGATCGAAGAGGAAGGATTTCGAATGGCACATTAGCAACGTGGAGACTTGGGTGAGCGCGGCGATGACGGACGGGAACACTTGCTCCGATGGATTTGCAGGGGCGGTGCTGAATGGAAGAATTAAATCATCGATTAGGCGTCGCATTGTGGATTTAACTCGATTCATAAGTAATTCCTTGGCTCTGATTAACAACTATGCTCAAAATCAAAGCTAG

Coding sequence (CDS)

ATGGCTAAATCCGCCTTCCTTCTCCTACTCCTCTCTATCTCCATCCTATTCGCCACCTCCAGCGCTGCCGACTTCGGCGGAGCTTCCAATTTCATCAAATCGAGATGCAATTTCACAACGTACCCTGACCTATGCGTTCAATCCCTCACAAATTTCGCCTCAAGAATCCAACAAAACCCTCGCCAATTAGTCCAAACCGCTCTAATGGTGAGCCTTTCTCACGCTCGATCCGCCCGATCCTTCGTCTGGAAACTGACGAAGTTCACCGGATTGAAGCCGCGAGAGTACGCGGCGTTGAGAGACTGTCTAGAGGAGGTCGGCGACACGGTGGAACGGCTGAGCAAATCGGTGGAAGAACTGAAGCTGGTTAGGAGATCGAAGAGGAAGGATTTCGAATGGCACATTAGCAACGTGGAGACTTGGGTGAGCGCGGCGATGACGGACGGGAACACTTGCTCCGATGGATTTGCAGGGGCGGTGCTGAATGGAAGAATTAAATCATCGATTAGGCGTCGCATTGTGGATTTAACTCGATTCATAAGTAATTCCTTGGCTCTGATTAACAACTATGCTCAAAATCAAAGCTAG

Protein sequence

MAKSAFLLLLLSISILFATSSAADFGGASNFIKSRCNFTTYPDLCVQSLTNFASRIQQNPRQLVQTALMVSLSHARSARSFVWKLTKFTGLKPREYAALRDCLEEVGDTVERLSKSVEELKLVRRSKRKDFEWHISNVETWVSAAMTDGNTCSDGFAGAVLNGRIKSSIRRRIVDLTRFISNSLALINNYAQNQS
Homology
[Close]
BLAST of Cp4.1LG01g03820 vs. Swiss-Prot
Match: 21KD_DAUCA (21 kDa protein OS=Daucus carota PE=2 SV=1)

HSP 1 Score: 182.6 bits (462), Expect = 4.2e-45
Identity = 93/191 (48.69%), Postives = 136/191 (71.20%), Query Frame = 1 

Query: 1   MAKSAFLLLLLSISILFATSSAADFGGASNFIKSRCNFTTYPDLCVQSLTNFASRIQQNP 60
           M  S   L+  ++ ++ A +SAA    A+ FIK+ C  TTYP +C QSL+ +A  IQ NP
Sbjct: 1   MKLSKSTLVFSALLVILAAASAAP---ANQFIKTSCTLTTYPAVCEQSLSAYAKTIQNNP 60

Query: 61  RQLVQTALMVSLSHARSARSFVWKLTKFTGLKPREYAALRDCLEEVGDTVERLSKSVEEL 120
           ++L  TAL VSL+  + A++F+ +L KF GLK R+YAA+ DCLEEV D+++R+S+S +E+
Sbjct: 61  QELASTALQVSLTRTQQAQTFMKRLNKFKGLKARQYAAIHDCLEEVEDSLDRVSRSCDEM 120

Query: 121 KLVRRSKRKDFEWHISNVETWVSAAMTDGNTCSDGFAGAVLNGRIKSSIRRRIVDLTRFI 180
           K +  +K  DF + +SNVETWVSAA+TD  TC DGFAG  ++G+IK S+R ++V + R  
Sbjct: 121 KNLSHAKGNDFTFRMSNVETWVSAALTDETTCMDGFAGKGMDGKIKESVRAQVVAVARVT 180

Query: 181 SNSLALINNYA 192
           SN+LAL+NN+A
Sbjct: 181 SNALALVNNFA 188

[Close]
BLAST of Cp4.1LG01g03820 vs. Swiss-Prot
Match: PME3_CITSI (Pectinesterase 3 OS=Citrus sinensis PE=1 SV=1)

HSP 1 Score: 87.4 bits (215), Expect = 1.8e-16
Identity = 58/193 (30.05%), Postives = 101/193 (52.33%), Query Frame = 1 

Query: 4   SAFLLLLLSISILFATSSAADFGGASN-----FIKSRCNFTTYPDLCVQSLTNF--ASRI 63
           +  L++   I I+   +S  + G   N      +KS C+ T YPDLC  ++     AS+ 
Sbjct: 36  ATLLVVAAVIGIVAGVNSRKNSGDNGNEPHHAILKSSCSSTRYPDLCFSAIAAVPEASKK 95

Query: 64  QQNPRQLVQTALMVSLSHARSARSFVWKLTKFTGLKPREYAALRDCLEEVGDTVERLSKS 123
             + + +++ +L ++ +        + KL K T L  RE  AL DCLE + +T++ L K+
Sbjct: 96  VTSQKDVIEMSLNITTTAVEHNYFGIQKLLKRTNLTKREKVALHDCLETIDETLDELHKA 155

Query: 124 VEELKLVRRSKRKDFEWHISNVETWVSAAMTDGNTCSDGFAGAVLNGRIKSSIRRRIVDL 183
           VE+L+      +K    H  +++T +SAAMT+  TC DGF+    N  ++ ++    V +
Sbjct: 156 VEDLE--EYPNKKSLSQHADDLKTLMSAAMTNQGTCLDGFSHDDANKHVRDALSDGQVHV 215

Query: 184 TRFISNSLALINN 190
            +  SN+LA+I N
Sbjct: 216 EKMCSNALAMIKN 226

[Close]
BLAST of Cp4.1LG01g03820 vs. Swiss-Prot
Match: PME1_CITSI (Pectinesterase 1 OS=Citrus sinensis GN=PECS-1.1 PE=2 SV=1)

HSP 1 Score: 87.4 bits (215), Expect = 1.8e-16
Identity = 58/193 (30.05%), Postives = 101/193 (52.33%), Query Frame = 1 

Query: 4   SAFLLLLLSISILFATSSAADFGGASN-----FIKSRCNFTTYPDLCVQSLTNF--ASRI 63
           +  L++   I I+   +S  + G   N      +KS C+ T YPDLC  ++     AS+ 
Sbjct: 36  ATLLVVAAVIGIVAGVNSRKNSGDNGNEPHHAILKSSCSSTRYPDLCFSAIAAVPEASKK 95

Query: 64  QQNPRQLVQTALMVSLSHARSARSFVWKLTKFTGLKPREYAALRDCLEEVGDTVERLSKS 123
             + + +++ +L ++ +        + KL K T L  RE  AL DCLE + +T++ L K+
Sbjct: 96  VTSQKDVIEMSLNITTTAVEHNYFGIQKLLKRTNLTKREKVALHDCLETIDETLDELHKA 155

Query: 124 VEELKLVRRSKRKDFEWHISNVETWVSAAMTDGNTCSDGFAGAVLNGRIKSSIRRRIVDL 183
           VE+L+      +K    H  +++T +SAAMT+  TC DGF+    N  ++ ++    V +
Sbjct: 156 VEDLE--EYPNKKSLSQHADDLKTLMSAAMTNQGTCLDGFSHDDANKHVRDALSDGQVHV 215

Query: 184 TRFISNSLALINN 190
            +  SN+LA+I N
Sbjct: 216 EKMCSNALAMIKN 226

[Close]
BLAST of Cp4.1LG01g03820 vs. Swiss-Prot
Match: PME3_ARATH (Pectinesterase/pectinesterase inhibitor 3 OS=Arabidopsis thaliana GN=PME3 PE=2 SV=2)

HSP 1 Score: 82.8 bits (203), Expect = 4.6e-15
Identity = 53/197 (26.90%), Postives = 104/197 (52.79%), Query Frame = 1 

Query: 7   LLLLLSISILFATSSAADFGGASN-------------FIKSRCNFTTYPDLCVQSLTNFA 66
           +LL  ++++LF  + A    GAS               ++S C+ T YP+LC+ ++    
Sbjct: 21  VLLSAAVALLFVAAVAGISAGASKANEKRTLSPSSHAVLRSSCSSTRYPELCISAVVTAG 80

Query: 67  SRIQQNPRQLVQTALMVSLSHARSARSFVWKLTKFT-GLKPREYAALRDCLEEVGDTVER 126
                + + +++ ++ ++++        V KL K   GL PRE  AL DCLE + +T++ 
Sbjct: 81  GVELTSQKDVIEASVNLTITAVEHNYFTVKKLIKKRKGLTPREKTALHDCLETIDETLDE 140

Query: 127 LSKSVEELKLVRRSKRKDFEWHISNVETWVSAAMTDGNTCSDGFAGAVLNGRIKSSIRRR 186
           L ++VE+L L     +K    H  +++T +S+A+T+  TC DGF+    + +++ ++ + 
Sbjct: 141 LHETVEDLHLY--PTKKTLREHAGDLKTLISSAITNQETCLDGFSHDDADKQVRKALLKG 200

Query: 187 IVDLTRFISNSLALINN 190
            + +    SN+LA+I N
Sbjct: 201 QIHVEHMCSNALAMIKN 215

[Close]
BLAST of Cp4.1LG01g03820 vs. Swiss-Prot
Match: PMEU1_SOLLC (Pectinesterase/pectinesterase inhibitor U1 OS=Solanum lycopersicum GN=PMEU1 PE=2 SV=1)

HSP 1 Score: 82.8 bits (203), Expect = 4.6e-15
Identity = 49/165 (29.70%), Postives = 95/165 (57.58%), Query Frame = 1 

Query: 28  ASNFIKSRCNFTTYPDLCVQSLTNFA--SRIQQNPRQLVQTALMVSLSHARSARSFVWKL 87
           A   +KS C+ T +P+LC  ++ N +  S+   + + +++ +L +++   R     V +L
Sbjct: 62  AHAIVKSACSNTLHPELCYSAIVNVSDFSKKVTSQKDVIELSLNITVKAVRRNYYAVKEL 121

Query: 88  TKFT-GLKPREYAALRDCLEEVGDTVERLSKSVEELKLVRRSKRKDFEWHISNVETWVSA 147
            K   GL PRE  AL DCLE + +T++ L  +VE+L+L     +K  + H+ +++T +S+
Sbjct: 122 IKTRKGLTPREKVALHDCLETMDETLDELHTAVEDLELY--PNKKSLKEHVEDLKTLISS 181

Query: 148 AMTDGNTCSDGFAGAVLNGRIKSSIRRRIVDLTRFISNSLALINN 190
           A+T+  TC DGF+    + +++  + +    + +  SN+LA+I N
Sbjct: 182 AITNQETCLDGFSHDEADKKVRKVLLKGQKHVEKMCSNALAMICN 224

[Close]
BLAST of Cp4.1LG01g03820 vs. TrEMBL
Match: A0A0A0L8D7_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_3G176260 PE=4 SV=1)

HSP 1 Score: 278.1 bits (710), Expect = 8.2e-72
Identity = 149/195 (76.41%), Postives = 173/195 (88.72%), Query Frame = 1 

Query: 1   MAKSAFLLLLLSISILFATSSAADFGGASNFIKSRCNFTTYPDLCVQSLTNFASRIQQNP 60
           MAKS  LLLLL +SIL  ++SA + GGAS+FIKS+C+  TYPDLCVQSL++F+S IQ+NP
Sbjct: 1   MAKSLSLLLLL-LSILTISASAFN-GGASSFIKSKCSAATYPDLCVQSLSSFSSTIQRNP 60

Query: 61  RQLVQTALMVSLSHARSARSFVWKLTKFTGLKPREYAALRDCLEEVGDTVERLSKSVEEL 120
           RQLVQTAL VSLSHA+S RSFVWKLTKF+GLKPRE AAL+DC+EEVGDTV+RL+KSVEEL
Sbjct: 61  RQLVQTALAVSLSHAQSTRSFVWKLTKFSGLKPRERAALKDCMEEVGDTVDRLNKSVEEL 120

Query: 121 KLVRRSKRKDFEWHISNVETWVSAAMTDGNTCSDGFAGAVLNGRIKSSIRRRIVDLTRFI 180
           K V  SK KDF+WHISNVETWVSAAMTD NTCSDGFAG+ LNGRIKSS+R RIVD+TR I
Sbjct: 121 KRVSGSK-KDFQWHISNVETWVSAAMTDENTCSDGFAGSALNGRIKSSVRGRIVDVTRVI 180

Query: 181 SNSLALINNYAQNQS 196
           SN+L+LIN YA+NQS
Sbjct: 181 SNALSLINKYAENQS 192

[Close]
BLAST of Cp4.1LG01g03820 vs. TrEMBL
Match: B9R9Q7_RICCO (21 kDa protein, putative OS=Ricinus communis GN=RCOM_1500010 PE=4 SV=1)

HSP 1 Score: 226.5 bits (576), Expect = 2.8e-56
Identity = 118/193 (61.14%), Postives = 149/193 (77.20%), Query Frame = 1 

Query: 1   MAKSAFLLLLLSISILFATS--SAADFGGASNFIKSRCNFTTYPDLCVQSLTNFASRIQQ 60
           M K   LLLL+S+ +  +++  SAA    ASNFIK+ C  TTYP LC+QSL+ +A+ IQQ
Sbjct: 1   MVKPTLLLLLVSVLLYISSTATSAAAPAAASNFIKASCRTTTYPALCIQSLSAYATSIQQ 60

Query: 61  NPRQLVQTALMVSLSHARSARSFVWKLTKFTGLKPREYAALRDCLEEVGDTVERLSKSVE 120
           NP QL QTAL VSL +A+S R+FV+KLTKF G+KPRE AAL+DCLEE+ DT +RLSKS  
Sbjct: 61  NPLQLTQTALSVSLENAQSTRTFVYKLTKFKGVKPREMAALKDCLEEIDDTADRLSKSYN 120

Query: 121 ELKLVRRSKRKDFEWHISNVETWVSAAMTDGNTCSDGFAGAVLNGRIKSSIRRRIVDLTR 180
           ELK +   K KDF+WH+SNVETWVSAA+TD NTC+DGFAG  LNG++KSSI+ RIV + +
Sbjct: 121 ELKNLGHYKGKDFQWHMSNVETWVSAALTDENTCTDGFAGKALNGKMKSSIKARIVKVAQ 180

Query: 181 FISNSLALINNYA 192
             SN+L+LIN YA
Sbjct: 181 VTSNALSLINKYA 193

[Close]
BLAST of Cp4.1LG01g03820 vs. TrEMBL
Match: A9PDL3_POPTR (21K protein OS=Populus trichocarpa GN=POPTR_0012s14560g PE=2 SV=1)

HSP 1 Score: 221.1 bits (562), Expect = 1.2e-54
Identity = 115/194 (59.28%), Postives = 148/194 (76.29%), Query Frame = 1 

Query: 1   MAKSAFLLLLLSISILFATSSAADF----GGASNFIKSRCNFTTYPDLCVQSLTNFASRI 60
           MAKS+ +LLLLSI  +  T+++A        A+NFIK+ C  TTYP LCVQSL+ +A+ I
Sbjct: 1   MAKSSLVLLLLSILYIAGTATSASTTSSSASATNFIKASCTATTYPALCVQSLSLYATSI 60

Query: 61  QQNPRQLVQTALMVSLSHARSARSFVWKLTKFTGLKPREYAALRDCLEEVGDTVERLSKS 120
            Q+PRQL+QTAL VSL  A+S ++FV+KLTKF G+KPRE AA++DC EE+ DTV+RL KS
Sbjct: 61  NQSPRQLIQTALAVSLDKAQSTKTFVYKLTKFKGVKPREKAAIKDCFEEIDDTVDRLVKS 120

Query: 121 VEELKLVRRSKRKDFEWHISNVETWVSAAMTDGNTCSDGFAGAVLNGRIKSSIRRRIVDL 180
           V+ELK +  SK  DF+WHISNV+TW+SA +TD NTC DGFAG  LNGRIK+SI+ R V +
Sbjct: 121 VKELKNMGSSKGSDFQWHISNVQTWISAGLTDENTCVDGFAGKALNGRIKNSIKARFVHV 180

Query: 181 TRFISNSLALINNY 191
            R  SN+LALIN +
Sbjct: 181 ERVTSNALALINKF 194

[Close]
BLAST of Cp4.1LG01g03820 vs. TrEMBL
Match: M5X6V6_PRUPE (Uncharacterized protein OS=Prunus persica GN=PRUPE_ppa011831mg PE=4 SV=1)

HSP 1 Score: 219.5 bits (558), Expect = 3.5e-54
Identity = 109/191 (57.07%), Postives = 150/191 (78.53%), Query Frame = 1 

Query: 1   MAKSAFLLLLLSISILFATSSAADFGGASNFIKSRCNFTTYPDLCVQSLTNFASRIQQNP 60
           MA+++F LLLL I     T+++      +NFIKS C+ TTYP +C+QSL+++A+ IQQ+P
Sbjct: 1   MARASFFLLLLPIFYFVGTANSTATTSDTNFIKSSCSATTYPQVCIQSLSSYATSIQQSP 60

Query: 61  RQLVQTALMVSLSHARSARSFVWKLTKFTGLKPREYAALRDCLEEVGDTVERLSKSVEEL 120
           RQL Q AL VSL+ A+ A++FV KL KF GLK REY A++DCLEE+GDTV+RLSKSV+EL
Sbjct: 61  RQLAQAALSVSLTRAQKAKTFVTKLAKFKGLKAREYGAVKDCLEEMGDTVDRLSKSVQEL 120

Query: 121 KLVRRSKRKDFEWHISNVETWVSAAMTDGNTCSDGFAGAVLNGRIKSSIRRRIVDLTRFI 180
           K + +SK +DF WH+SNVETWVSAA+TD NTC DGF+G  L+G+IK+SIR +++++ +  
Sbjct: 121 KNMGKSKGQDFVWHMSNVETWVSAALTDDNTCLDGFSGKALDGKIKASIRAQVLNVAQCT 180

Query: 181 SNSLALINNYA 192
           SN+LAL N +A
Sbjct: 181 SNALALCNRFA 191

[Close]
BLAST of Cp4.1LG01g03820 vs. TrEMBL
Match: A9PD34_POPTR (Putative uncharacterized protein OS=Populus trichocarpa PE=2 SV=1)

HSP 1 Score: 218.8 bits (556), Expect = 5.9e-54
Identity = 114/194 (58.76%), Postives = 147/194 (75.77%), Query Frame = 1 

Query: 1   MAKSAFLLLLLSISILFATSSAADF----GGASNFIKSRCNFTTYPDLCVQSLTNFASRI 60
           MAKS+ +LLLLSI  +  T+++A        A+NFIK+ C  TTYP LCVQSL+ +A+ I
Sbjct: 1   MAKSSLVLLLLSILYIAGTATSASTTSSSASATNFIKASCTATTYPALCVQSLSLYATSI 60

Query: 61  QQNPRQLVQTALMVSLSHARSARSFVWKLTKFTGLKPREYAALRDCLEEVGDTVERLSKS 120
            Q+PRQL+QTAL VSL  A+S ++FV+KLTKF G+KPRE AA++DC EE+ DTV+RL KS
Sbjct: 61  NQSPRQLIQTALAVSLDKAQSTKTFVYKLTKFKGVKPREKAAIKDCFEEIDDTVDRLVKS 120

Query: 121 VEELKLVRRSKRKDFEWHISNVETWVSAAMTDGNTCSDGFAGAVLNGRIKSSIRRRIVDL 180
           V+ELK +  SK  DF+WHISNV+TW+SA +TD  TC DGFAG  LNGRIK+SI+ R V +
Sbjct: 121 VKELKNMGSSKGSDFQWHISNVQTWISAGLTDEKTCVDGFAGKALNGRIKNSIKARFVHV 180

Query: 181 TRFISNSLALINNY 191
            R  SN+LALIN +
Sbjct: 181 ERVTSNALALINKF 194

[Close]
BLAST of Cp4.1LG01g03820 vs. TAIR10
Match: AT3G47380.1 (AT3G47380.1 Plant invertase/pectin methylesterase inhibitor superfamily protein)

HSP 1 Score: 187.2 bits (474), Expect = 9.7e-48
Identity = 101/200 (50.50%), Postives = 143/200 (71.50%), Query Frame = 1 

Query: 1   MAKSAF---LLLLLSISILFATSSAADFGGAS----NFIKSRCNFTTYPDLCVQSLTNFA 60
           MAK  F    L LLS +IL A+SSA      S    NFI++ C  TTYP +CV SLT +A
Sbjct: 1   MAKQIFYTLFLFLLSTAILTASSSAPRAAITSKRAINFIQASCKATTYPTVCVNSLTGYA 60

Query: 61  SRIQQNPRQLVQTALMVSLSHARSARSFVWKLTKFTGLKPREYAALRDCLEEVGDTVERL 120
           + IQ +PR+L +TAL V+++ A+S + FVW+L +FT LK RE  A++DC+EE+ D V+RL
Sbjct: 61  NSIQTSPRRLAETALNVTVTQAQSTKVFVWRLGRFTSLKKREIQAVKDCIEEIHDAVDRL 120

Query: 121 SKSVEELKLVRRSKRKDFEW-HISNVETWVSAAMTDGNTCSDGFAGAVLNGRIKSSIRRR 180
           + S+ E+K+   +K +D  W H+SN +TW SAA+T+ NTCSDGFAG V++GR+K+S+R R
Sbjct: 121 TMSIHEVKMCGSAKGRDQFWFHMSNAQTWTSAALTNANTCSDGFAGRVMDGRVKNSVRAR 180

Query: 181 IVDLTRFISNSLALINNYAQ 193
           I++L R  SN+LALIN +A+
Sbjct: 181 ILNLGRGTSNALALINAFAK 200

[Close]
BLAST of Cp4.1LG01g03820 vs. TAIR10
Match: AT5G62350.1 (AT5G62350.1 Plant invertase/pectin methylesterase inhibitor superfamily protein)

HSP 1 Score: 185.3 bits (469), Expect = 3.7e-47
Identity = 104/201 (51.74%), Postives = 148/201 (73.63%), Query Frame = 1 

Query: 1   MAKSAFLLLLLSISILFA----TSSAADFGGAS----NFIKSRCNFTTYPDLCVQSLTNF 60
           MAK  +L +LLSIS L +     ++AA   GAS    NFI+S C  TTYP LCV SL+ +
Sbjct: 1   MAKQ-YLFVLLSISYLLSLELTAATAASQTGASKKAINFIQSSCKTTTYPALCVHSLSVY 60

Query: 61  ASRIQQNPRQLVQTALMVSLSHARSARSFVWKLTKFTGLKPREYAALRDCLEEVGDTVER 120
           A+ IQ +P++L +TA+ V+LS A+S + FV +LT+  GLK RE  A++DC+EE+ DTV+R
Sbjct: 61  ANDIQTSPKRLAETAIAVTLSRAQSTKLFVSRLTRMKGLKKREVEAIKDCVEEMNDTVDR 120

Query: 121 LSKSVEELKLVRRSKRKD-FEWHISNVETWVSAAMTDGNTCSDGFAGAVLNGRIKSSIRR 180
           L+KSV+ELKL   +K +D F +H+SN +TW SAA+TD NTCSDGF+G V++GRIK+S+R 
Sbjct: 121 LTKSVQELKLCGSAKDQDQFAYHMSNAQTWTSAALTDENTCSDGFSGRVMDGRIKNSVRA 180

Query: 181 RIVDLTRFISNSLALINNYAQ 193
           RI+++    SN+L+LIN +A+
Sbjct: 181 RIMNVGHETSNALSLINAFAK 200

[Close]
BLAST of Cp4.1LG01g03820 vs. TAIR10
Match: AT4G12390.1 (AT4G12390.1 pectin methylesterase inhibitor 1)

HSP 1 Score: 166.4 bits (420), Expect = 1.8e-41
Identity = 92/186 (49.46%), Postives = 124/186 (66.67%), Query Frame = 1 

Query: 8   LLLLSISILFATSSAADFGGASNFIKSRCNFTTYPDLCVQSLTNFASRIQQNPRQLVQTA 67
           L LL  S +   SS+ +   + NFI S C  T Y  LCV+ L  FA +I++N  QL QTA
Sbjct: 16  LPLLCQSTIAKPSSSPNPSSSINFIVSSCRVTRYQTLCVKCLAAFADKIRRNENQLAQTA 75

Query: 68  LMVSLSHARSARSFVWKLTKFTGLKPREYAALRDCLEEVGDTVERLSKSVEELKLVRRS- 127
           L V+L   +S   +V KLTK   +K REY A++DC+E +GD +E L++S+ ELK V RS 
Sbjct: 76  LAVTLVRVQSTTIYVGKLTKARRIKRREYLAVKDCVENLGDGLEMLAQSMRELKQVGRSG 135

Query: 128 -KRKDFEWHISNVETWVSAAMTDGNTCSDGFAGAVLNGRIKSSIRRRIVDLTRFISNSLA 187
             R +F W +SNVETWVSAA+TD  TC DGF G V++G +KS+IRRR+V + R  SN+LA
Sbjct: 136 RDRDEFLWRLSNVETWVSAALTDETTCLDGFDGKVMDGVVKSAIRRRVVHVARVTSNALA 195

Query: 188 LINNYA 192
           L+N +A
Sbjct: 196 LVNRFA 201

[Close]
BLAST of Cp4.1LG01g03820 vs. TAIR10
Match: AT4G25260.1 (AT4G25260.1 Plant invertase/pectin methylesterase inhibitor superfamily protein)

HSP 1 Score: 166.0 bits (419), Expect = 2.3e-41
Identity = 86/187 (45.99%), Postives = 129/187 (68.98%), Query Frame = 1 

Query: 6   FLLLLLSISILFATSSAADFGGASNFIKSRCNFTTYPDLCVQSLTNFASRIQQNPRQLVQ 65
           F+L L + +I+ A +   +  G + FIK+ C  T+YPD C QSL+++AS I++ PR+L +
Sbjct: 12  FVLYLSTAAIVMARNLEEESSGDTEFIKASCETTSYPDRCFQSLSSYASEIKKQPRKLAE 71

Query: 66  TALMVSLSHARSARSFVWKLTKFTGLKPREYAALRDCLEEVGDTVERLSKSVEELK-LVR 125
           TAL VS++ A+SA+++V ++T + G+  R++ A+ DCLEE+GDTV+RLS S++ELK L  
Sbjct: 72  TALAVSIARAKSAKTYVSEMTDYKGITKRQHEAVADCLEEMGDTVDRLSNSLKELKHLEE 131

Query: 126 RSKRKDFEWHISNVETWVSAAMTDGNTCSDGFAGAVLNGRIKSSIRRRIVDLTRFISNSL 185
               +DF + +SNV TW SAA+TD   C DGF G  + G +KS IR  IV +    SN+L
Sbjct: 132 GDSGEDFWFCLSNVRTWTSAALTDETACMDGFGGKAMAGELKSLIRTHIVSVAEETSNAL 191

Query: 186 ALINNYA 192
           ALIN++A
Sbjct: 192 ALINDFA 198

[Close]
BLAST of Cp4.1LG01g03820 vs. TAIR10
Match: AT1G62770.1 (AT1G62770.1 Plant invertase/pectin methylesterase inhibitor superfamily protein)

HSP 1 Score: 159.8 bits (403), Expect = 1.7e-39
Identity = 88/198 (44.44%), Postives = 136/198 (68.69%), Query Frame = 1 

Query: 1   MAKSAFLLLLLSISILFATSSAADFGGASNFIKSRCNFTTYPDLCVQSLTNFASRIQQNP 60
           +  + FL++LLSI+IL ++S+  +   +  FI S C  T YP LCV +L+ +A++I+ N 
Sbjct: 3   LKNTIFLVILLSITILQSSSATPNRSESDQFIVSSCQTTQYPSLCVHTLSAYATKIRHNN 62

Query: 61  RQ-LVQTALMVSLSHARSARSFVWKLTKFTG-LKPREYAALRDCLEEVGDTVERLSKSVE 120
            Q L QTAL++SL+ A+S   FV KLTK T   K REY A++DC+E +G++V+RL++SV+
Sbjct: 63  DQDLAQTALIISLARAKSVTIFVAKLTKETPKFKRREYLAIKDCIEVLGNSVDRLAQSVK 122

Query: 121 ELKLVRRS-KRKDFEWHISNVETWVSAAMTDGNTCSDGFAGAVLNGRIKSSIRRRIVDLT 180
           EL     +   +DF W +SNV+TWVSAA+TD  TC DGF+   + G++K  IR ++V + 
Sbjct: 123 ELARAGHAVASEDFMWKMSNVQTWVSAALTDETTCLDGFSERAMGGKVKRLIRYKVVHVA 182

Query: 181 RFISNSLALINNYAQNQS 196
           +  SN+LAL+N +A+ +S
Sbjct: 183 QVTSNALALVNQFAEKRS 200

[Close]
BLAST of Cp4.1LG01g03820 vs. NCBI nr
Match: gi|659077241|ref|XP_008439100.1| (PREDICTED: 21 kDa protein-like [Cucumis melo])

HSP 1 Score: 279.3 bits (713), Expect = 5.3e-72
Identity = 149/195 (76.41%), Postives = 171/195 (87.69%), Query Frame = 1 

Query: 1   MAKSAFLLLLLSISILFATSSAADFGGASNFIKSRCNFTTYPDLCVQSLTNFASRIQQNP 60
           MAKS  LLLLL +SIL  ++SA + GGASNFIKS+C+  TYPDLCVQSL++FAS IQ+NP
Sbjct: 1   MAKSLSLLLLL-LSILTISASAFN-GGASNFIKSKCSAATYPDLCVQSLSSFASTIQRNP 60

Query: 61  RQLVQTALMVSLSHARSARSFVWKLTKFTGLKPREYAALRDCLEEVGDTVERLSKSVEEL 120
           RQLVQTAL VSLS A+S RSFVWKLTKF+GLKPRE AAL+DC+EEVGDTV+RL+KSVEEL
Sbjct: 61  RQLVQTALAVSLSRAQSTRSFVWKLTKFSGLKPRERAALKDCMEEVGDTVDRLNKSVEEL 120

Query: 121 KLVRRSKRKDFEWHISNVETWVSAAMTDGNTCSDGFAGAVLNGRIKSSIRRRIVDLTRFI 180
           K V  SK+KDF WHISNVETWVSAAMTD NTCSDGFAG+ LNGRIKSS+R RIVD+TR I
Sbjct: 121 KRVSGSKKKDFLWHISNVETWVSAAMTDENTCSDGFAGSALNGRIKSSVRGRIVDVTRVI 180

Query: 181 SNSLALINNYAQNQS 196
           SN+L+LIN YA+ QS
Sbjct: 181 SNALSLINKYAETQS 193

[Close]
BLAST of Cp4.1LG01g03820 vs. NCBI nr
Match: gi|449460808|ref|XP_004148136.1| (PREDICTED: 21 kDa protein-like [Cucumis sativus])

HSP 1 Score: 278.1 bits (710), Expect = 1.2e-71
Identity = 149/195 (76.41%), Postives = 173/195 (88.72%), Query Frame = 1 

Query: 1   MAKSAFLLLLLSISILFATSSAADFGGASNFIKSRCNFTTYPDLCVQSLTNFASRIQQNP 60
           MAKS  LLLLL +SIL  ++SA + GGAS+FIKS+C+  TYPDLCVQSL++F+S IQ+NP
Sbjct: 1   MAKSLSLLLLL-LSILTISASAFN-GGASSFIKSKCSAATYPDLCVQSLSSFSSTIQRNP 60

Query: 61  RQLVQTALMVSLSHARSARSFVWKLTKFTGLKPREYAALRDCLEEVGDTVERLSKSVEEL 120
           RQLVQTAL VSLSHA+S RSFVWKLTKF+GLKPRE AAL+DC+EEVGDTV+RL+KSVEEL
Sbjct: 61  RQLVQTALAVSLSHAQSTRSFVWKLTKFSGLKPRERAALKDCMEEVGDTVDRLNKSVEEL 120

Query: 121 KLVRRSKRKDFEWHISNVETWVSAAMTDGNTCSDGFAGAVLNGRIKSSIRRRIVDLTRFI 180
           K V  SK KDF+WHISNVETWVSAAMTD NTCSDGFAG+ LNGRIKSS+R RIVD+TR I
Sbjct: 121 KRVSGSK-KDFQWHISNVETWVSAAMTDENTCSDGFAGSALNGRIKSSVRGRIVDVTRVI 180

Query: 181 SNSLALINNYAQNQS 196
           SN+L+LIN YA+NQS
Sbjct: 181 SNALSLINKYAENQS 192

[Close]
BLAST of Cp4.1LG01g03820 vs. NCBI nr
Match: gi|255539735|ref|XP_002510932.1| (PREDICTED: 21 kDa protein [Ricinus communis])

HSP 1 Score: 226.5 bits (576), Expect = 4.1e-56
Identity = 118/193 (61.14%), Postives = 149/193 (77.20%), Query Frame = 1 

Query: 1   MAKSAFLLLLLSISILFATS--SAADFGGASNFIKSRCNFTTYPDLCVQSLTNFASRIQQ 60
           M K   LLLL+S+ +  +++  SAA    ASNFIK+ C  TTYP LC+QSL+ +A+ IQQ
Sbjct: 1   MVKPTLLLLLVSVLLYISSTATSAAAPAAASNFIKASCRTTTYPALCIQSLSAYATSIQQ 60

Query: 61  NPRQLVQTALMVSLSHARSARSFVWKLTKFTGLKPREYAALRDCLEEVGDTVERLSKSVE 120
           NP QL QTAL VSL +A+S R+FV+KLTKF G+KPRE AAL+DCLEE+ DT +RLSKS  
Sbjct: 61  NPLQLTQTALSVSLENAQSTRTFVYKLTKFKGVKPREMAALKDCLEEIDDTADRLSKSYN 120

Query: 121 ELKLVRRSKRKDFEWHISNVETWVSAAMTDGNTCSDGFAGAVLNGRIKSSIRRRIVDLTR 180
           ELK +   K KDF+WH+SNVETWVSAA+TD NTC+DGFAG  LNG++KSSI+ RIV + +
Sbjct: 121 ELKNLGHYKGKDFQWHMSNVETWVSAALTDENTCTDGFAGKALNGKMKSSIKARIVKVAQ 180

Query: 181 FISNSLALINNYA 192
             SN+L+LIN YA
Sbjct: 181 VTSNALSLINKYA 193

[Close]
BLAST of Cp4.1LG01g03820 vs. NCBI nr
Match: gi|224120250|ref|XP_002318283.1| (21K protein precursor [Populus trichocarpa])

HSP 1 Score: 221.1 bits (562), Expect = 1.7e-54
Identity = 115/194 (59.28%), Postives = 148/194 (76.29%), Query Frame = 1 

Query: 1   MAKSAFLLLLLSISILFATSSAADF----GGASNFIKSRCNFTTYPDLCVQSLTNFASRI 60
           MAKS+ +LLLLSI  +  T+++A        A+NFIK+ C  TTYP LCVQSL+ +A+ I
Sbjct: 1   MAKSSLVLLLLSILYIAGTATSASTTSSSASATNFIKASCTATTYPALCVQSLSLYATSI 60

Query: 61  QQNPRQLVQTALMVSLSHARSARSFVWKLTKFTGLKPREYAALRDCLEEVGDTVERLSKS 120
            Q+PRQL+QTAL VSL  A+S ++FV+KLTKF G+KPRE AA++DC EE+ DTV+RL KS
Sbjct: 61  NQSPRQLIQTALAVSLDKAQSTKTFVYKLTKFKGVKPREKAAIKDCFEEIDDTVDRLVKS 120

Query: 121 VEELKLVRRSKRKDFEWHISNVETWVSAAMTDGNTCSDGFAGAVLNGRIKSSIRRRIVDL 180
           V+ELK +  SK  DF+WHISNV+TW+SA +TD NTC DGFAG  LNGRIK+SI+ R V +
Sbjct: 121 VKELKNMGSSKGSDFQWHISNVQTWISAGLTDENTCVDGFAGKALNGRIKNSIKARFVHV 180

Query: 181 TRFISNSLALINNY 191
            R  SN+LALIN +
Sbjct: 181 ERVTSNALALINKF 194

[Close]
BLAST of Cp4.1LG01g03820 vs. NCBI nr
Match: gi|657944738|ref|XP_008376261.1| (PREDICTED: 21 kDa protein [Malus domestica])

HSP 1 Score: 219.5 bits (558), Expect = 5.0e-54
Identity = 109/193 (56.48%), Postives = 150/193 (77.72%), Query Frame = 1 

Query: 1   MAKSAFLLLLLSISILFATSSAADFGGASNFIKSRCNFTTYPDLCVQSLTNFASRIQQNP 60
           MA+++  LLLL I  L  T+ +A     +NFI++ C  TTYP +C+QSL+++A+ IQQ+P
Sbjct: 1   MARASVFLLLLPIFYLVGTAKSASTTTTTNFIETSCRATTYPQVCIQSLSSYANSIQQSP 60

Query: 61  RQLVQTALMVSLSHARSARSFVWKLTKFTGLKPREYAALRDCLEEVGDTVERLSKSVEEL 120
           RQL Q AL VSL+ A+SA++FV KL KF GLK REY A++DCLEE+GD+V+R+SKSV EL
Sbjct: 61  RQLAQAALSVSLTKAKSAKTFVTKLAKFKGLKSREYGAIKDCLEEMGDSVDRMSKSVTEL 120

Query: 121 KLVRRSKRKDFEWHISNVETWVSAAMTDGNTCSDGFAGAVLNGRIKSSIRRRIVDLTRFI 180
           K + +SK +DF WH+SNVETWVSAA+TD NTC DGF+G  LNG+IKSS+R +++++ +  
Sbjct: 121 KNMGKSKGQDFLWHMSNVETWVSAALTDDNTCLDGFSGKALNGKIKSSVRAQVLNVAQCT 180

Query: 181 SNSLALINNYAQN 194
           SN+LAL N +A N
Sbjct: 181 SNALALCNRFAGN 193

The following BLAST results are available for this feature:
BLAST of Cp4.1LG01g03820 vs. Swiss-Prot
Match NameE-valueIdentityDescription
21KD_DAUCA4.2e-4548.6921 kDa protein OS=Daucus carota PE=2 SV=1[more]
PME3_CITSI1.8e-1630.05Pectinesterase 3 OS=Citrus sinensis PE=1 SV=1[more]
PME1_CITSI1.8e-1630.05Pectinesterase 1 OS=Citrus sinensis GN=PECS-1.1 PE=2 SV=1[more]
PME3_ARATH4.6e-1526.90Pectinesterase/pectinesterase inhibitor 3 OS=Arabidopsis thaliana GN=PME3 PE=2 S... [more]
PMEU1_SOLLC4.6e-1529.70Pectinesterase/pectinesterase inhibitor U1 OS=Solanum lycopersicum GN=PMEU1 PE=2... [more]
BLAST of Cp4.1LG01g03820 vs. TrEMBL
Match NameE-valueIdentityDescription
A0A0A0L8D7_CUCSA8.2e-7276.41Uncharacterized protein OS=Cucumis sativus GN=Csa_3G176260 PE=4 SV=1[more]
B9R9Q7_RICCO2.8e-5661.1421 kDa protein, putative OS=Ricinus communis GN=RCOM_1500010 PE=4 SV=1[more]
A9PDL3_POPTR1.2e-5459.2821K protein OS=Populus trichocarpa GN=POPTR_0012s14560g PE=2 SV=1[more]
M5X6V6_PRUPE3.5e-5457.07Uncharacterized protein OS=Prunus persica GN=PRUPE_ppa011831mg PE=4 SV=1[more]
A9PD34_POPTR5.9e-5458.76Putative uncharacterized protein OS=Populus trichocarpa PE=2 SV=1[more]
BLAST of Cp4.1LG01g03820 vs. TAIR10
Match NameE-valueIdentityDescription
AT3G47380.19.7e-4850.50 Plant invertase/pectin methylesterase inhibitor superfamily protein[more]
AT5G62350.13.7e-4751.74 Plant invertase/pectin methylesterase inhibitor superfamily protein[more]
AT4G12390.11.8e-4149.46 pectin methylesterase inhibitor 1[more]
AT4G25260.12.3e-4145.99 Plant invertase/pectin methylesterase inhibitor superfamily protein[more]
AT1G62770.11.7e-3944.44 Plant invertase/pectin methylesterase inhibitor superfamily protein[more]
BLAST of Cp4.1LG01g03820 vs. NCBI nr
Match NameE-valueIdentityDescription
gi|659077241|ref|XP_008439100.1|5.3e-7276.41PREDICTED: 21 kDa protein-like [Cucumis melo][more]
gi|449460808|ref|XP_004148136.1|1.2e-7176.41PREDICTED: 21 kDa protein-like [Cucumis sativus][more]
gi|255539735|ref|XP_002510932.1|4.1e-5661.14PREDICTED: 21 kDa protein [Ricinus communis][more]
gi|224120250|ref|XP_002318283.1|1.7e-5459.2821K protein precursor [Populus trichocarpa][more]
gi|657944738|ref|XP_008376261.1|5.0e-5456.48PREDICTED: 21 kDa protein [Malus domestica][more]
Annotated Terms
The following terms have been associated with this gene:
Vocabulary: Molecular Function
TermDefinition
GO:0004857enzyme inhibitor activity
Vocabulary: INTERPRO
TermDefinition
IPR006501Pectinesterase_inhib_dom
GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0043086 negative regulation of catalytic activity
biological_process GO:0005982 starch metabolic process
biological_process GO:0005985 sucrose metabolic process
biological_process GO:0008150 biological_process
cellular_component GO:0005575 cellular_component
molecular_function GO:0004857 enzyme inhibitor activity
molecular_function GO:0030599 pectinesterase activity
Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Cp4.1LG01g03820.1Cp4.1LG01g03820.1mRNA


InterPro
Analysis Name: InterPro Annotations of Cucurbita pepo
Date Performed: 2017-12-02
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR006501Pectinesterase inhibitor domainGENE3DG3DSA:1.20.140.40coord: 29..189
score: 7.2
IPR006501Pectinesterase inhibitor domainPFAMPF04043PMEIcoord: 30..186
score: 1.3
IPR006501Pectinesterase inhibitor domainSMARTSM00856PMEI_2coord: 27..186
score: 7.6
IPR006501Pectinesterase inhibitor domainTIGRFAMsTIGR01614TIGR01614coord: 1..190
score: 2.6
IPR006501Pectinesterase inhibitor domainunknownSSF101148Plant invertase/pectin methylesterase inhibitorcoord: 29..190
score: 5.36
NoneNo IPR availablePANTHERPTHR31080FAMILY NOT NAMEDcoord: 6..192
score: 6.3
NoneNo IPR availablePANTHERPTHR31080:SF9INVERTASE/PECTIN METHYLESTERASE INHIBITOR FAMILY PROTEIN / DC 1.2-LIKE PROTEIN-RELATEDcoord: 6..192
score: 6.3