CmaCh16G001840 (gene) Cucurbita maxima (Rimu)

Overview
NameCmaCh16G001840
Typegene
OrganismCucurbita maxima (Cucurbita maxima (Rimu))
DescriptionPlant invertase/pectin methylesterase inhibitor superfamily protein
LocationCma_Chr16 : 811696 .. 812510 (+)
Genome Viewer
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCGAAATCCGCCTCCCTTCTCCTACTCCTCTCCATTCTCACCATCGCCGCCACCACCAGCGCCGCTCGTAACGGCGGAGCTTCCAATTTCATCAAATCGAAGTGCAGCGCCGCAACTTACCCTGACCTCTGCGTCCAATCCCTCTCAACCTTCGCTTCCACAATCCAACAAAACCCTCGCCAATTAGTCCAAACCGCCCTAGTCGTGAGCCTCTCACGCGCTCAATCCACCCGATCATTCGTCTGGAAAATGACGAAGTTCAGTGGCTTGAAGAAGAGAGAGTATGCGGCCTTGAGGGATTGTATGGAGGAGGTCGGCGACACCGTGGATCGCCTAAGCAAATCAGTCGAGGAACTGAAGCGCGTTAGCGGATCGAAGCAGAAGGACTTCCAATGGCACATGAGCAACGTGGAGACTTGGGTGAGCGCTGCCATGACCGACGAAAACACTTGCTCCGACGGATTTGCCGGCTCGGCGCTGAACGGAAGAATTAAGTCTTCGATCAGAGCTCGCATCGTGGATGTTTCTCGAGTCATAAGCAATGCGTTGTCTCTGATTAACAAGTACGCTGAAAATGAAAGTTAGAGATGTGGTTTCAGGTTCTTCGAATCCCTACACTGCTTCTGCGTCTGAAGATGAATTAGATGAATCTGTCAAGAGTTTGATGTATATTAAGAGAGCTTTTGAGTGTTTTGAAGTGGTAAATGCAACCATAACGATGTAGTGCTTGCTCTGTTTTTGGGTATTCTCTGTGCAACCGTAATAGATACTCTTAGTTTCGGTTTTGTCTTAAACAGAAAAGTTTTAGCTAA

mRNA sequence

ATGGCGAAATCCGCCTCCCTTCTCCTACTCCTCTCCATTCTCACCATCGCCGCCACCACCAGCGCCGCTCGTAACGGCGGAGCTTCCAATTTCATCAAATCGAAGTGCAGCGCCGCAACTTACCCTGACCTCTGCGTCCAATCCCTCTCAACCTTCGCTTCCACAATCCAACAAAACCCTCGCCAATTAGTCCAAACCGCCCTAGTCGTGAGCCTCTCACGCGCTCAATCCACCCGATCATTCGTCTGGAAAATGACGAAGTTCAGTGGCTTGAAGAAGAGAGAGTATGCGGCCTTGAGGGATTGTATGGAGGAGGTCGGCGACACCGTGGATCGCCTAAGCAAATCAGTCGAGGAACTGAAGCGCGTTAGCGGATCGAAGCAGAAGGACTTCCAATGGCACATGAGCAACGTGGAGACTTGGGTGAGCGCTGCCATGACCGACGAAAACACTTGCTCCGACGGATTTGCCGGCTCGGCGCTGAACGGAAGAATTAAGTCTTCGATCAGAGCTCGCATCGTGGATGTTTCTCGAGTCATAAGCAATGCGTTGTCTCTGATTAACAAGTACGCTGAAAATGAAAGTTCTTCGAATCCCTACACTGCTTCTGCGTCTGAAGATGAATTAGATGAATCTGTCAAGAGTTTGATGTATATTAAGAGAGCTTTTGAGTGTTTTGAAGTGAAAAGTTTTAGCTAA

Coding sequence (CDS)

ATGGCGAAATCCGCCTCCCTTCTCCTACTCCTCTCCATTCTCACCATCGCCGCCACCACCAGCGCCGCTCGTAACGGCGGAGCTTCCAATTTCATCAAATCGAAGTGCAGCGCCGCAACTTACCCTGACCTCTGCGTCCAATCCCTCTCAACCTTCGCTTCCACAATCCAACAAAACCCTCGCCAATTAGTCCAAACCGCCCTAGTCGTGAGCCTCTCACGCGCTCAATCCACCCGATCATTCGTCTGGAAAATGACGAAGTTCAGTGGCTTGAAGAAGAGAGAGTATGCGGCCTTGAGGGATTGTATGGAGGAGGTCGGCGACACCGTGGATCGCCTAAGCAAATCAGTCGAGGAACTGAAGCGCGTTAGCGGATCGAAGCAGAAGGACTTCCAATGGCACATGAGCAACGTGGAGACTTGGGTGAGCGCTGCCATGACCGACGAAAACACTTGCTCCGACGGATTTGCCGGCTCGGCGCTGAACGGAAGAATTAAGTCTTCGATCAGAGCTCGCATCGTGGATGTTTCTCGAGTCATAAGCAATGCGTTGTCTCTGATTAACAAGTACGCTGAAAATGAAAGTTCTTCGAATCCCTACACTGCTTCTGCGTCTGAAGATGAATTAGATGAATCTGTCAAGAGTTTGATGTATATTAAGAGAGCTTTTGAGTGTTTTGAAGTGAAAAGTTTTAGCTAA

Protein sequence

MAKSASLLLLLSILTIAATTSAARNGGASNFIKSKCSAATYPDLCVQSLSTFASTIQQNPRQLVQTALVVSLSRAQSTRSFVWKMTKFSGLKKREYAALRDCMEEVGDTVDRLSKSVEELKRVSGSKQKDFQWHMSNVETWVSAAMTDENTCSDGFAGSALNGRIKSSIRARIVDVSRVISNALSLINKYAENESSSNPYTASASEDELDESVKSLMYIKRAFECFEVKSFS
Homology
[Close]
BLAST of CmaCh16G001840 vs. Swiss-Prot
Match: 21KD_DAUCA (21 kDa protein OS=Daucus carota PE=2 SV=1)

HSP 1 Score: 195.3 bits (495), Expect = 7.5e-49
Identity = 102/191 (53.40%), Postives = 141/191 (73.82%), Query Frame = 1 

Query: 1   MAKSASLLLLLSILTIAATTSAARNGGASNFIKSKCSAATYPDLCVQSLSTFASTIQQNP 60
           M  S S L+  ++L I A  SAA    A+ FIK+ C+  TYP +C QSLS +A TIQ NP
Sbjct: 1   MKLSKSTLVFSALLVILAAASAAP---ANQFIKTSCTLTTYPAVCEQSLSAYAKTIQNNP 60

Query: 61  RQLVQTALVVSLSRAQSTRSFVWKMTKFSGLKKREYAALRDCMEEVGDTVDRLSKSVEEL 120
           ++L  TAL VSL+R Q  ++F+ ++ KF GLK R+YAA+ DC+EEV D++DR+S+S +E+
Sbjct: 61  QELASTALQVSLTRTQQAQTFMKRLNKFKGLKARQYAAIHDCLEEVEDSLDRVSRSCDEM 120

Query: 121 KRVSGSKQKDFQWHMSNVETWVSAAMTDENTCSDGFAGSALNGRIKSSIRARIVDVSRVI 180
           K +S +K  DF + MSNVETWVSAA+TDE TC DGFAG  ++G+IK S+RA++V V+RV 
Sbjct: 121 KNLSHAKGNDFTFRMSNVETWVSAALTDETTCMDGFAGKGMDGKIKESVRAQVVAVARVT 180

Query: 181 SNALSLINKYA 192
           SNAL+L+N +A
Sbjct: 181 SNALALVNNFA 188

[Close]
BLAST of CmaCh16G001840 vs. Swiss-Prot
Match: PME1_CITSI (Pectinesterase 1 OS=Citrus sinensis GN=PECS-1.1 PE=2 SV=1)

HSP 1 Score: 95.1 bits (235), Expect = 1.1e-18
Identity = 59/202 (29.21%), Postives = 113/202 (55.94%), Query Frame = 1 

Query: 1   MAKSASLLLLLSILTIAATTSAARNGGASN------FIKSKCSAATYPDLCVQSLSTF-- 60
           +A  A+LL++ +++ I A  ++ +N G +        +KS CS+  YPDLC  +++    
Sbjct: 32  LALFATLLVVAAVIGIVAGVNSRKNSGDNGNEPHHAILKSSCSSTRYPDLCFSAIAAVPE 91

Query: 61  ASTIQQNPRQLVQTALVVSLSRAQSTRSFVWKMTKFSGLKKREYAALRDCMEEVGDTVDR 120
           AS    + + +++ +L ++ +  +     + K+ K + L KRE  AL DC+E + +T+D 
Sbjct: 92  ASKKVTSQKDVIEMSLNITTTAVEHNYFGIQKLLKRTNLTKREKVALHDCLETIDETLDE 151

Query: 121 LSKSVEELKRVSGSKQKDFQWHMSNVETWVSAAMTDENTCSDGFAGSALNGRIKSSIRAR 180
           L K+VE+L+      +K    H  +++T +SAAMT++ TC DGF+    N  ++ ++   
Sbjct: 152 LHKAVEDLEEYPN--KKSLSQHADDLKTLMSAAMTNQGTCLDGFSHDDANKHVRDALSDG 211

Query: 181 IVDVSRVISNALSLINKYAENE 195
            V V ++ SNAL++I    + +
Sbjct: 212 QVHVEKMCSNALAMIKNMTDTD 231

[Close]
BLAST of CmaCh16G001840 vs. Swiss-Prot
Match: PME3_CITSI (Pectinesterase 3 OS=Citrus sinensis PE=1 SV=1)

HSP 1 Score: 95.1 bits (235), Expect = 1.1e-18
Identity = 59/202 (29.21%), Postives = 113/202 (55.94%), Query Frame = 1 

Query: 1   MAKSASLLLLLSILTIAATTSAARNGGASN------FIKSKCSAATYPDLCVQSLSTF-- 60
           +A  A+LL++ +++ I A  ++ +N G +        +KS CS+  YPDLC  +++    
Sbjct: 32  LALFATLLVVAAVIGIVAGVNSRKNSGDNGNEPHHAILKSSCSSTRYPDLCFSAIAAVPE 91

Query: 61  ASTIQQNPRQLVQTALVVSLSRAQSTRSFVWKMTKFSGLKKREYAALRDCMEEVGDTVDR 120
           AS    + + +++ +L ++ +  +     + K+ K + L KRE  AL DC+E + +T+D 
Sbjct: 92  ASKKVTSQKDVIEMSLNITTTAVEHNYFGIQKLLKRTNLTKREKVALHDCLETIDETLDE 151

Query: 121 LSKSVEELKRVSGSKQKDFQWHMSNVETWVSAAMTDENTCSDGFAGSALNGRIKSSIRAR 180
           L K+VE+L+      +K    H  +++T +SAAMT++ TC DGF+    N  ++ ++   
Sbjct: 152 LHKAVEDLEEYPN--KKSLSQHADDLKTLMSAAMTNQGTCLDGFSHDDANKHVRDALSDG 211

Query: 181 IVDVSRVISNALSLINKYAENE 195
            V V ++ SNAL++I    + +
Sbjct: 212 QVHVEKMCSNALAMIKNMTDTD 231

[Close]
BLAST of CmaCh16G001840 vs. Swiss-Prot
Match: PME2_ARATH (Pectinesterase 2 OS=Arabidopsis thaliana GN=PME2 PE=2 SV=2)

HSP 1 Score: 87.4 bits (215), Expect = 2.2e-16
Identity = 59/198 (29.80%), Postives = 108/198 (54.55%), Query Frame = 1 

Query: 6   SLLLLLSILTIAATTS--------AARNGGASNFIKSKCSAATYPDLCVQSLSTFASTIQ 65
           +LLLL SI+ IAATT+           +  +   +KS CS+  YP+LC  +++       
Sbjct: 28  ALLLLASIVGIAATTTNQNKNQKITTLSSTSHAILKSVCSSTLYPELCFSAVAATGGKEL 87

Query: 66  QNPRQLVQTALVVSLSRAQSTRSFVWKM-TKFSGLKKREYAALRDCMEEVGDTVDRLSKS 125
            + +++++ +L ++    +     V K+  K  GL  RE  AL DC+E + +T+D L  +
Sbjct: 88  TSQKEVIEASLNLTTKAVKHNYFAVKKLIAKRKGLTPREVTALHDCLETIDETLDELHVA 147

Query: 126 VEELKRVSGSKQKDFQWHMSNVETWVSAAMTDENTCSDGFAGSALNGRIKSSIRARIVDV 185
           VE+L +    KQK  + H  +++T +S+A+T++ TC DGF+    + +++ ++    V V
Sbjct: 148 VEDLHQYP--KQKSLRKHADDLKTLISSAITNQGTCLDGFSYDDADRKVRKALLKGQVHV 207

Query: 186 SRVISNALSLINKYAENE 195
             + SNAL++I    E +
Sbjct: 208 EHMCSNALAMIKNMTETD 223

[Close]
BLAST of CmaCh16G001840 vs. Swiss-Prot
Match: PME3_ARATH (Pectinesterase/pectinesterase inhibitor 3 OS=Arabidopsis thaliana GN=PME3 PE=2 SV=2)

HSP 1 Score: 76.3 bits (186), Expect = 5.1e-13
Identity = 49/200 (24.50%), Postives = 109/200 (54.50%), Query Frame = 1 

Query: 1   MAKSASLLLLLSILTIAATTSAAR-----NGGASNFIKSKCSAATYPDLCVQSLSTFAST 60
           ++ + +LL + ++  I+A  S A      +  +   ++S CS+  YP+LC+ ++ T    
Sbjct: 23  LSAAVALLFVAAVAGISAGASKANEKRTLSPSSHAVLRSSCSSTRYPELCISAVVTAGGV 82

Query: 61  IQQNPRQLVQTALVVSLSRAQSTRSFVWKMTKF-SGLKKREYAALRDCMEEVGDTVDRLS 120
              + + +++ ++ ++++  +     V K+ K   GL  RE  AL DC+E + +T+D L 
Sbjct: 83  ELTSQKDVIEASVNLTITAVEHNYFTVKKLIKKRKGLTPREKTALHDCLETIDETLDELH 142

Query: 121 KSVEELKRVSGSKQKDFQWHMSNVETWVSAAMTDENTCSDGFAGSALNGRIKSSIRARIV 180
           ++VE+L       +K  + H  +++T +S+A+T++ TC DGF+    + +++ ++    +
Sbjct: 143 ETVEDLHLYP--TKKTLREHAGDLKTLISSAITNQETCLDGFSHDDADKQVRKALLKGQI 202

Query: 181 DVSRVISNALSLINKYAENE 195
            V  + SNAL++I    + +
Sbjct: 203 HVEHMCSNALAMIKNMTDTD 220

[Close]
BLAST of CmaCh16G001840 vs. TrEMBL
Match: A0A0A0L8D7_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_3G176260 PE=4 SV=1)

HSP 1 Score: 308.9 bits (790), Expect = 5.2e-81
Identity = 171/196 (87.24%), Postives = 184/196 (93.88%), Query Frame = 1 

Query: 1   MAKSASLLLLL-SILTIAATTSAARNGGASNFIKSKCSAATYPDLCVQSLSTFASTIQQN 60
           MAKS SLLLLL SILTI+A+   A NGGAS+FIKSKCSAATYPDLCVQSLS+F+STIQ+N
Sbjct: 1   MAKSLSLLLLLLSILTISAS---AFNGGASSFIKSKCSAATYPDLCVQSLSSFSSTIQRN 60

Query: 61  PRQLVQTALVVSLSRAQSTRSFVWKMTKFSGLKKREYAALRDCMEEVGDTVDRLSKSVEE 120
           PRQLVQTAL VSLS AQSTRSFVWK+TKFSGLK RE AAL+DCMEEVGDTVDRL+KSVEE
Sbjct: 61  PRQLVQTALAVSLSHAQSTRSFVWKLTKFSGLKPRERAALKDCMEEVGDTVDRLNKSVEE 120

Query: 121 LKRVSGSKQKDFQWHMSNVETWVSAAMTDENTCSDGFAGSALNGRIKSSIRARIVDVSRV 180
           LKRVSGSK KDFQWH+SNVETWVSAAMTDENTCSDGFAGSALNGRIKSS+R RIVDV+RV
Sbjct: 121 LKRVSGSK-KDFQWHISNVETWVSAAMTDENTCSDGFAGSALNGRIKSSVRGRIVDVTRV 180

Query: 181 ISNALSLINKYAENES 196
           ISNALSLINKYAEN+S
Sbjct: 181 ISNALSLINKYAENQS 192

[Close]
BLAST of CmaCh16G001840 vs. TrEMBL
Match: B9R9Q7_RICCO (21 kDa protein, putative OS=Ricinus communis GN=RCOM_1500010 PE=4 SV=1)

HSP 1 Score: 243.4 bits (620), Expect = 2.7e-61
Identity = 130/193 (67.36%), Postives = 154/193 (79.79%), Query Frame = 1 

Query: 1   MAKSASLLLLLSILTIAATT--SAARNGGASNFIKSKCSAATYPDLCVQSLSTFASTIQQ 60
           M K   LLLL+S+L   ++T  SAA    ASNFIK+ C   TYP LC+QSLS +A++IQQ
Sbjct: 1   MVKPTLLLLLVSVLLYISSTATSAAAPAAASNFIKASCRTTTYPALCIQSLSAYATSIQQ 60

Query: 61  NPRQLVQTALVVSLSRAQSTRSFVWKMTKFSGLKKREYAALRDCMEEVGDTVDRLSKSVE 120
           NP QL QTAL VSL  AQSTR+FV+K+TKF G+K RE AAL+DC+EE+ DT DRLSKS  
Sbjct: 61  NPLQLTQTALSVSLENAQSTRTFVYKLTKFKGVKPREMAALKDCLEEIDDTADRLSKSYN 120

Query: 121 ELKRVSGSKQKDFQWHMSNVETWVSAAMTDENTCSDGFAGSALNGRIKSSIRARIVDVSR 180
           ELK +   K KDFQWHMSNVETWVSAA+TDENTC+DGFAG ALNG++KSSI+ARIV V++
Sbjct: 121 ELKNLGHYKGKDFQWHMSNVETWVSAALTDENTCTDGFAGKALNGKMKSSIKARIVKVAQ 180

Query: 181 VISNALSLINKYA 192
           V SNALSLINKYA
Sbjct: 181 VTSNALSLINKYA 193

[Close]
BLAST of CmaCh16G001840 vs. TrEMBL
Match: A9PDL3_POPTR (21K protein OS=Populus trichocarpa GN=POPTR_0012s14560g PE=2 SV=1)

HSP 1 Score: 241.5 bits (615), Expect = 1.0e-60
Identity = 126/194 (64.95%), Postives = 158/194 (81.44%), Query Frame = 1 

Query: 1   MAKSASLLLLLSILTIAATTSAAR----NGGASNFIKSKCSAATYPDLCVQSLSTFASTI 60
           MAKS+ +LLLLSIL IA T ++A     +  A+NFIK+ C+A TYP LCVQSLS +A++I
Sbjct: 1   MAKSSLVLLLLSILYIAGTATSASTTSSSASATNFIKASCTATTYPALCVQSLSLYATSI 60

Query: 61  QQNPRQLVQTALVVSLSRAQSTRSFVWKMTKFSGLKKREYAALRDCMEEVGDTVDRLSKS 120
            Q+PRQL+QTAL VSL +AQST++FV+K+TKF G+K RE AA++DC EE+ DTVDRL KS
Sbjct: 61  NQSPRQLIQTALAVSLDKAQSTKTFVYKLTKFKGVKPREKAAIKDCFEEIDDTVDRLVKS 120

Query: 121 VEELKRVSGSKQKDFQWHMSNVETWVSAAMTDENTCSDGFAGSALNGRIKSSIRARIVDV 180
           V+ELK +  SK  DFQWH+SNV+TW+SA +TDENTC DGFAG ALNGRIK+SI+AR V V
Sbjct: 121 VKELKNMGSSKGSDFQWHISNVQTWISAGLTDENTCVDGFAGKALNGRIKNSIKARFVHV 180

Query: 181 SRVISNALSLINKY 191
            RV SNAL+LINK+
Sbjct: 181 ERVTSNALALINKF 194

[Close]
BLAST of CmaCh16G001840 vs. TrEMBL
Match: A9PD34_POPTR (Putative uncharacterized protein OS=Populus trichocarpa PE=2 SV=1)

HSP 1 Score: 239.2 bits (609), Expect = 5.0e-60
Identity = 125/194 (64.43%), Postives = 157/194 (80.93%), Query Frame = 1 

Query: 1   MAKSASLLLLLSILTIAATTSAAR----NGGASNFIKSKCSAATYPDLCVQSLSTFASTI 60
           MAKS+ +LLLLSIL IA T ++A     +  A+NFIK+ C+A TYP LCVQSLS +A++I
Sbjct: 1   MAKSSLVLLLLSILYIAGTATSASTTSSSASATNFIKASCTATTYPALCVQSLSLYATSI 60

Query: 61  QQNPRQLVQTALVVSLSRAQSTRSFVWKMTKFSGLKKREYAALRDCMEEVGDTVDRLSKS 120
            Q+PRQL+QTAL VSL +AQST++FV+K+TKF G+K RE AA++DC EE+ DTVDRL KS
Sbjct: 61  NQSPRQLIQTALAVSLDKAQSTKTFVYKLTKFKGVKPREKAAIKDCFEEIDDTVDRLVKS 120

Query: 121 VEELKRVSGSKQKDFQWHMSNVETWVSAAMTDENTCSDGFAGSALNGRIKSSIRARIVDV 180
           V+ELK +  SK  DFQWH+SNV+TW+SA +TDE TC DGFAG ALNGRIK+SI+AR V V
Sbjct: 121 VKELKNMGSSKGSDFQWHISNVQTWISAGLTDEKTCVDGFAGKALNGRIKNSIKARFVHV 180

Query: 181 SRVISNALSLINKY 191
            RV SNAL+LINK+
Sbjct: 181 ERVTSNALALINKF 194

[Close]
BLAST of CmaCh16G001840 vs. TrEMBL
Match: A9PBM0_POPTR (21K protein OS=Populus trichocarpa GN=POPTR_0015s14640g PE=2 SV=1)

HSP 1 Score: 233.4 bits (594), Expect = 2.8e-58
Identity = 121/195 (62.05%), Postives = 158/195 (81.03%), Query Frame = 1 

Query: 1   MAKSASLLLLLSILTIAATTSAAR-----NGGASNFIKSKCSAATYPDLCVQSLSTFAST 60
           MAK + + LL+S+L IA+T + A      +  ASNFIK+ CSA TYP LCVQSLS +AS+
Sbjct: 1   MAKPSLIWLLISLLYIASTANCASTTTKSSSRASNFIKASCSATTYPALCVQSLSLYASS 60

Query: 61  IQQNPRQLVQTALVVSLSRAQSTRSFVWKMTKFSGLKKREYAALRDCMEEVGDTVDRLSK 120
           I+Q+PRQL+QTAL VSL +AQST++FV+K+TKF G+K RE AA++DC EE+ DT+D+L K
Sbjct: 61  IKQSPRQLIQTALSVSLDKAQSTKTFVYKLTKFKGIKPREKAAIKDCFEEIDDTLDKLVK 120

Query: 121 SVEELKRVSGSKQKDFQWHMSNVETWVSAAMTDENTCSDGFAGSALNGRIKSSIRARIVD 180
           SV+ELK +  SK +D+QWH+SNV+TW+SAA+TDENTC DGFAG AL+GR+K+SI AR V 
Sbjct: 121 SVKELKNMGSSKGQDYQWHISNVQTWISAALTDENTCVDGFAGKALDGRVKNSITARFVH 180

Query: 181 VSRVISNALSLINKY 191
           V RV SNAL+LINK+
Sbjct: 181 VERVTSNALALINKF 195

[Close]
BLAST of CmaCh16G001840 vs. TAIR10
Match: AT5G62350.1 (AT5G62350.1 Plant invertase/pectin methylesterase inhibitor superfamily protein)

HSP 1 Score: 199.5 bits (506), Expect = 2.2e-51
Identity = 108/195 (55.38%), Postives = 146/195 (74.87%), Query Frame = 1 

Query: 7   LLLLLSI-----LTIAATTSAARNGG---ASNFIKSKCSAATYPDLCVQSLSTFASTIQQ 66
           L +LLSI     L + A T+A++ G    A NFI+S C   TYP LCV SLS +A+ IQ 
Sbjct: 6   LFVLLSISYLLSLELTAATAASQTGASKKAINFIQSSCKTTTYPALCVHSLSVYANDIQT 65

Query: 67  NPRQLVQTALVVSLSRAQSTRSFVWKMTKFSGLKKREYAALRDCMEEVGDTVDRLSKSVE 126
           +P++L +TA+ V+LSRAQST+ FV ++T+  GLKKRE  A++DC+EE+ DTVDRL+KSV+
Sbjct: 66  SPKRLAETAIAVTLSRAQSTKLFVSRLTRMKGLKKREVEAIKDCVEEMNDTVDRLTKSVQ 125

Query: 127 ELKRVSGSKQKD-FQWHMSNVETWVSAAMTDENTCSDGFAGSALNGRIKSSIRARIVDVS 186
           ELK    +K +D F +HMSN +TW SAA+TDENTCSDGF+G  ++GRIK+S+RARI++V 
Sbjct: 126 ELKLCGSAKDQDQFAYHMSNAQTWTSAALTDENTCSDGFSGRVMDGRIKNSVRARIMNVG 185

Query: 187 RVISNALSLINKYAE 193
              SNALSLIN +A+
Sbjct: 186 HETSNALSLINAFAK 200

[Close]
BLAST of CmaCh16G001840 vs. TAIR10
Match: AT3G47380.1 (AT3G47380.1 Plant invertase/pectin methylesterase inhibitor superfamily protein)

HSP 1 Score: 185.3 bits (469), Expect = 4.4e-47
Identity = 96/190 (50.53%), Postives = 143/190 (75.26%), Query Frame = 1 

Query: 8   LLLLSILTIAATTSAAR----NGGASNFIKSKCSAATYPDLCVQSLSTFASTIQQNPRQL 67
           L LLS   + A++SA R    +  A NFI++ C A TYP +CV SL+ +A++IQ +PR+L
Sbjct: 11  LFLLSTAILTASSSAPRAAITSKRAINFIQASCKATTYPTVCVNSLTGYANSIQTSPRRL 70

Query: 68  VQTALVVSLSRAQSTRSFVWKMTKFSGLKKREYAALRDCMEEVGDTVDRLSKSVEELKRV 127
            +TAL V++++AQST+ FVW++ +F+ LKKRE  A++DC+EE+ D VDRL+ S+ E+K  
Sbjct: 71  AETALNVTVTQAQSTKVFVWRLGRFTSLKKREIQAVKDCIEEIHDAVDRLTMSIHEVKMC 130

Query: 128 SGSKQKD-FQWHMSNVETWVSAAMTDENTCSDGFAGSALNGRIKSSIRARIVDVSRVISN 187
             +K +D F +HMSN +TW SAA+T+ NTCSDGFAG  ++GR+K+S+RARI+++ R  SN
Sbjct: 131 GSAKGRDQFWFHMSNAQTWTSAALTNANTCSDGFAGRVMDGRVKNSVRARILNLGRGTSN 190

Query: 188 ALSLINKYAE 193
           AL+LIN +A+
Sbjct: 191 ALALINAFAK 200

[Close]
BLAST of CmaCh16G001840 vs. TAIR10
Match: AT4G25260.1 (AT4G25260.1 Plant invertase/pectin methylesterase inhibitor superfamily protein)

HSP 1 Score: 172.9 bits (437), Expect = 2.2e-43
Identity = 96/198 (48.48%), Postives = 137/198 (69.19%), Query Frame = 1 

Query: 1   MAKSASLLLLLSILTIA-ATTSAARN-----GGASNFIKSKCSAATYPDLCVQSLSTFAS 60
           MA++  L L+L +L ++ A    ARN      G + FIK+ C   +YPD C QSLS++AS
Sbjct: 1   MARNFELSLILFVLYLSTAAIVMARNLEEESSGDTEFIKASCETTSYPDRCFQSLSSYAS 60

Query: 61  TIQQNPRQLVQTALVVSLSRAQSTRSFVWKMTKFSGLKKREYAALRDCMEEVGDTVDRLS 120
            I++ PR+L +TAL VS++RA+S +++V +MT + G+ KR++ A+ DC+EE+GDTVDRLS
Sbjct: 61  EIKKQPRKLAETALAVSIARAKSAKTYVSEMTDYKGITKRQHEAVADCLEEMGDTVDRLS 120

Query: 121 KSVEELKRV-SGSKQKDFQWHMSNVETWVSAAMTDENTCSDGFAGSALNGRIKSSIRARI 180
            S++ELK +  G   +DF + +SNV TW SAA+TDE  C DGF G A+ G +KS IR  I
Sbjct: 121 NSLKELKHLEEGDSGEDFWFCLSNVRTWTSAALTDETACMDGFGGKAMAGELKSLIRTHI 180

Query: 181 VDVSRVISNALSLINKYA 192
           V V+   SNAL+LIN +A
Sbjct: 181 VSVAEETSNALALINDFA 198

[Close]
BLAST of CmaCh16G001840 vs. TAIR10
Match: AT4G12390.1 (AT4G12390.1 pectin methylesterase inhibitor 1)

HSP 1 Score: 172.2 bits (435), Expect = 3.8e-43
Identity = 96/195 (49.23%), Postives = 133/195 (68.21%), Query Frame = 1 

Query: 7   LLLLLSIL---TIAATTSAARNGGASNFIKSKCSAATYPDLCVQSLSTFASTIQQNPRQL 66
           LLL L +L   TIA  +S+     + NFI S C    Y  LCV+ L+ FA  I++N  QL
Sbjct: 12  LLLFLPLLCQSTIAKPSSSPNPSSSINFIVSSCRVTRYQTLCVKCLAAFADKIRRNENQL 71

Query: 67  VQTALVVSLSRAQSTRSFVWKMTKFSGLKKREYAALRDCMEEVGDTVDRLSKSVEELKRV 126
            QTAL V+L R QST  +V K+TK   +K+REY A++DC+E +GD ++ L++S+ ELK+V
Sbjct: 72  AQTALAVTLVRVQSTTIYVGKLTKARRIKRREYLAVKDCVENLGDGLEMLAQSMRELKQV 131

Query: 127 --SGSKQKDFQWHMSNVETWVSAAMTDENTCSDGFAGSALNGRIKSSIRARIVDVSRVIS 186
             SG  + +F W +SNVETWVSAA+TDE TC DGF G  ++G +KS+IR R+V V+RV S
Sbjct: 132 GRSGRDRDEFLWRLSNVETWVSAALTDETTCLDGFDGKVMDGVVKSAIRRRVVHVARVTS 191

Query: 187 NALSLINKYAENESS 197
           NAL+L+N++A    S
Sbjct: 192 NALALVNRFAARHKS 206

[Close]
BLAST of CmaCh16G001840 vs. TAIR10
Match: AT1G62770.1 (AT1G62770.1 Plant invertase/pectin methylesterase inhibitor superfamily protein)

HSP 1 Score: 165.6 bits (418), Expect = 3.6e-41
Identity = 92/196 (46.94%), Postives = 136/196 (69.39%), Query Frame = 1 

Query: 7   LLLLLSILTIAATTSAARNGGASNFIKSKCSAATYPDLCVQSLSTFASTIQQNPRQ-LVQ 66
           L++LLSI  + ++++      +  FI S C    YP LCV +LS +A+ I+ N  Q L Q
Sbjct: 9   LVILLSITILQSSSATPNRSESDQFIVSSCQTTQYPSLCVHTLSAYATKIRHNNDQDLAQ 68

Query: 67  TALVVSLSRAQSTRSFVWKMTKFSG-LKKREYAALRDCMEEVGDTVDRLSKSVEELKRVS 126
           TAL++SL+RA+S   FV K+TK +   K+REY A++DC+E +G++VDRL++SV+EL R  
Sbjct: 69  TALIISLARAKSVTIFVAKLTKETPKFKRREYLAIKDCIEVLGNSVDRLAQSVKELARAG 128

Query: 127 GS-KQKDFQWHMSNVETWVSAAMTDENTCSDGFAGSALNGRIKSSIRARIVDVSRVISNA 186
            +   +DF W MSNV+TWVSAA+TDE TC DGF+  A+ G++K  IR ++V V++V SNA
Sbjct: 129 HAVASEDFMWKMSNVQTWVSAALTDETTCLDGFSERAMGGKVKRLIRYKVVHVAQVTSNA 188

Query: 187 LSLINKYAENESSSNP 200
           L+L+N++AE  S   P
Sbjct: 189 LALVNQFAEKRSVKFP 204

[Close]
BLAST of CmaCh16G001840 vs. NCBI nr
Match: gi|659077241|ref|XP_008439100.1| (PREDICTED: 21 kDa protein-like [Cucumis melo])

HSP 1 Score: 313.9 bits (803), Expect = 2.3e-82
Identity = 172/196 (87.76%), Postives = 184/196 (93.88%), Query Frame = 1 

Query: 1   MAKSASLLLLL-SILTIAATTSAARNGGASNFIKSKCSAATYPDLCVQSLSTFASTIQQN 60
           MAKS SLLLLL SILTI+A+   A NGGASNFIKSKCSAATYPDLCVQSLS+FASTIQ+N
Sbjct: 1   MAKSLSLLLLLLSILTISAS---AFNGGASNFIKSKCSAATYPDLCVQSLSSFASTIQRN 60

Query: 61  PRQLVQTALVVSLSRAQSTRSFVWKMTKFSGLKKREYAALRDCMEEVGDTVDRLSKSVEE 120
           PRQLVQTAL VSLSRAQSTRSFVWK+TKFSGLK RE AAL+DCMEEVGDTVDRL+KSVEE
Sbjct: 61  PRQLVQTALAVSLSRAQSTRSFVWKLTKFSGLKPRERAALKDCMEEVGDTVDRLNKSVEE 120

Query: 121 LKRVSGSKQKDFQWHMSNVETWVSAAMTDENTCSDGFAGSALNGRIKSSIRARIVDVSRV 180
           LKRVSGSK+KDF WH+SNVETWVSAAMTDENTCSDGFAGSALNGRIKSS+R RIVDV+RV
Sbjct: 121 LKRVSGSKKKDFLWHISNVETWVSAAMTDENTCSDGFAGSALNGRIKSSVRGRIVDVTRV 180

Query: 181 ISNALSLINKYAENES 196
           ISNALSLINKYAE +S
Sbjct: 181 ISNALSLINKYAETQS 193

[Close]
BLAST of CmaCh16G001840 vs. NCBI nr
Match: gi|449460808|ref|XP_004148136.1| (PREDICTED: 21 kDa protein-like [Cucumis sativus])

HSP 1 Score: 308.9 bits (790), Expect = 7.4e-81
Identity = 171/196 (87.24%), Postives = 184/196 (93.88%), Query Frame = 1 

Query: 1   MAKSASLLLLL-SILTIAATTSAARNGGASNFIKSKCSAATYPDLCVQSLSTFASTIQQN 60
           MAKS SLLLLL SILTI+A+   A NGGAS+FIKSKCSAATYPDLCVQSLS+F+STIQ+N
Sbjct: 1   MAKSLSLLLLLLSILTISAS---AFNGGASSFIKSKCSAATYPDLCVQSLSSFSSTIQRN 60

Query: 61  PRQLVQTALVVSLSRAQSTRSFVWKMTKFSGLKKREYAALRDCMEEVGDTVDRLSKSVEE 120
           PRQLVQTAL VSLS AQSTRSFVWK+TKFSGLK RE AAL+DCMEEVGDTVDRL+KSVEE
Sbjct: 61  PRQLVQTALAVSLSHAQSTRSFVWKLTKFSGLKPRERAALKDCMEEVGDTVDRLNKSVEE 120

Query: 121 LKRVSGSKQKDFQWHMSNVETWVSAAMTDENTCSDGFAGSALNGRIKSSIRARIVDVSRV 180
           LKRVSGSK KDFQWH+SNVETWVSAAMTDENTCSDGFAGSALNGRIKSS+R RIVDV+RV
Sbjct: 121 LKRVSGSK-KDFQWHISNVETWVSAAMTDENTCSDGFAGSALNGRIKSSVRGRIVDVTRV 180

Query: 181 ISNALSLINKYAENES 196
           ISNALSLINKYAEN+S
Sbjct: 181 ISNALSLINKYAENQS 192

[Close]
BLAST of CmaCh16G001840 vs. NCBI nr
Match: gi|255539735|ref|XP_002510932.1| (PREDICTED: 21 kDa protein [Ricinus communis])

HSP 1 Score: 243.4 bits (620), Expect = 3.8e-61
Identity = 130/193 (67.36%), Postives = 154/193 (79.79%), Query Frame = 1 

Query: 1   MAKSASLLLLLSILTIAATT--SAARNGGASNFIKSKCSAATYPDLCVQSLSTFASTIQQ 60
           M K   LLLL+S+L   ++T  SAA    ASNFIK+ C   TYP LC+QSLS +A++IQQ
Sbjct: 1   MVKPTLLLLLVSVLLYISSTATSAAAPAAASNFIKASCRTTTYPALCIQSLSAYATSIQQ 60

Query: 61  NPRQLVQTALVVSLSRAQSTRSFVWKMTKFSGLKKREYAALRDCMEEVGDTVDRLSKSVE 120
           NP QL QTAL VSL  AQSTR+FV+K+TKF G+K RE AAL+DC+EE+ DT DRLSKS  
Sbjct: 61  NPLQLTQTALSVSLENAQSTRTFVYKLTKFKGVKPREMAALKDCLEEIDDTADRLSKSYN 120

Query: 121 ELKRVSGSKQKDFQWHMSNVETWVSAAMTDENTCSDGFAGSALNGRIKSSIRARIVDVSR 180
           ELK +   K KDFQWHMSNVETWVSAA+TDENTC+DGFAG ALNG++KSSI+ARIV V++
Sbjct: 121 ELKNLGHYKGKDFQWHMSNVETWVSAALTDENTCTDGFAGKALNGKMKSSIKARIVKVAQ 180

Query: 181 VISNALSLINKYA 192
           V SNALSLINKYA
Sbjct: 181 VTSNALSLINKYA 193

[Close]
BLAST of CmaCh16G001840 vs. NCBI nr
Match: gi|224120250|ref|XP_002318283.1| (21K protein precursor [Populus trichocarpa])

HSP 1 Score: 241.5 bits (615), Expect = 1.5e-60
Identity = 126/194 (64.95%), Postives = 158/194 (81.44%), Query Frame = 1 

Query: 1   MAKSASLLLLLSILTIAATTSAAR----NGGASNFIKSKCSAATYPDLCVQSLSTFASTI 60
           MAKS+ +LLLLSIL IA T ++A     +  A+NFIK+ C+A TYP LCVQSLS +A++I
Sbjct: 1   MAKSSLVLLLLSILYIAGTATSASTTSSSASATNFIKASCTATTYPALCVQSLSLYATSI 60

Query: 61  QQNPRQLVQTALVVSLSRAQSTRSFVWKMTKFSGLKKREYAALRDCMEEVGDTVDRLSKS 120
            Q+PRQL+QTAL VSL +AQST++FV+K+TKF G+K RE AA++DC EE+ DTVDRL KS
Sbjct: 61  NQSPRQLIQTALAVSLDKAQSTKTFVYKLTKFKGVKPREKAAIKDCFEEIDDTVDRLVKS 120

Query: 121 VEELKRVSGSKQKDFQWHMSNVETWVSAAMTDENTCSDGFAGSALNGRIKSSIRARIVDV 180
           V+ELK +  SK  DFQWH+SNV+TW+SA +TDENTC DGFAG ALNGRIK+SI+AR V V
Sbjct: 121 VKELKNMGSSKGSDFQWHISNVQTWISAGLTDENTCVDGFAGKALNGRIKNSIKARFVHV 180

Query: 181 SRVISNALSLINKY 191
            RV SNAL+LINK+
Sbjct: 181 ERVTSNALALINKF 194

[Close]
BLAST of CmaCh16G001840 vs. NCBI nr
Match: gi|118484840|gb|ABK94287.1| (unknown [Populus trichocarpa])

HSP 1 Score: 239.2 bits (609), Expect = 7.2e-60
Identity = 125/194 (64.43%), Postives = 157/194 (80.93%), Query Frame = 1 

Query: 1   MAKSASLLLLLSILTIAATTSAAR----NGGASNFIKSKCSAATYPDLCVQSLSTFASTI 60
           MAKS+ +LLLLSIL IA T ++A     +  A+NFIK+ C+A TYP LCVQSLS +A++I
Sbjct: 1   MAKSSLVLLLLSILYIAGTATSASTTSSSASATNFIKASCTATTYPALCVQSLSLYATSI 60

Query: 61  QQNPRQLVQTALVVSLSRAQSTRSFVWKMTKFSGLKKREYAALRDCMEEVGDTVDRLSKS 120
            Q+PRQL+QTAL VSL +AQST++FV+K+TKF G+K RE AA++DC EE+ DTVDRL KS
Sbjct: 61  NQSPRQLIQTALAVSLDKAQSTKTFVYKLTKFKGVKPREKAAIKDCFEEIDDTVDRLVKS 120

Query: 121 VEELKRVSGSKQKDFQWHMSNVETWVSAAMTDENTCSDGFAGSALNGRIKSSIRARIVDV 180
           V+ELK +  SK  DFQWH+SNV+TW+SA +TDE TC DGFAG ALNGRIK+SI+AR V V
Sbjct: 121 VKELKNMGSSKGSDFQWHISNVQTWISAGLTDEKTCVDGFAGKALNGRIKNSIKARFVHV 180

Query: 181 SRVISNALSLINKY 191
            RV SNAL+LINK+
Sbjct: 181 ERVTSNALALINKF 194

The following BLAST results are available for this feature:
BLAST of CmaCh16G001840 vs. Swiss-Prot
Match NameE-valueIdentityDescription
21KD_DAUCA7.5e-4953.4021 kDa protein OS=Daucus carota PE=2 SV=1[more]
PME1_CITSI1.1e-1829.21Pectinesterase 1 OS=Citrus sinensis GN=PECS-1.1 PE=2 SV=1[more]
PME3_CITSI1.1e-1829.21Pectinesterase 3 OS=Citrus sinensis PE=1 SV=1[more]
PME2_ARATH2.2e-1629.80Pectinesterase 2 OS=Arabidopsis thaliana GN=PME2 PE=2 SV=2[more]
PME3_ARATH5.1e-1324.50Pectinesterase/pectinesterase inhibitor 3 OS=Arabidopsis thaliana GN=PME3 PE=2 S... [more]
BLAST of CmaCh16G001840 vs. TrEMBL
Match NameE-valueIdentityDescription
A0A0A0L8D7_CUCSA5.2e-8187.24Uncharacterized protein OS=Cucumis sativus GN=Csa_3G176260 PE=4 SV=1[more]
B9R9Q7_RICCO2.7e-6167.3621 kDa protein, putative OS=Ricinus communis GN=RCOM_1500010 PE=4 SV=1[more]
A9PDL3_POPTR1.0e-6064.9521K protein OS=Populus trichocarpa GN=POPTR_0012s14560g PE=2 SV=1[more]
A9PD34_POPTR5.0e-6064.43Putative uncharacterized protein OS=Populus trichocarpa PE=2 SV=1[more]
A9PBM0_POPTR2.8e-5862.0521K protein OS=Populus trichocarpa GN=POPTR_0015s14640g PE=2 SV=1[more]
BLAST of CmaCh16G001840 vs. TAIR10
Match NameE-valueIdentityDescription
AT5G62350.12.2e-5155.38 Plant invertase/pectin methylesterase inhibitor superfamily protein[more]
AT3G47380.14.4e-4750.53 Plant invertase/pectin methylesterase inhibitor superfamily protein[more]
AT4G25260.12.2e-4348.48 Plant invertase/pectin methylesterase inhibitor superfamily protein[more]
AT4G12390.13.8e-4349.23 pectin methylesterase inhibitor 1[more]
AT1G62770.13.6e-4146.94 Plant invertase/pectin methylesterase inhibitor superfamily protein[more]
BLAST of CmaCh16G001840 vs. NCBI nr
Match NameE-valueIdentityDescription
gi|659077241|ref|XP_008439100.1|2.3e-8287.76PREDICTED: 21 kDa protein-like [Cucumis melo][more]
gi|449460808|ref|XP_004148136.1|7.4e-8187.24PREDICTED: 21 kDa protein-like [Cucumis sativus][more]
gi|255539735|ref|XP_002510932.1|3.8e-6167.36PREDICTED: 21 kDa protein [Ricinus communis][more]
gi|224120250|ref|XP_002318283.1|1.5e-6064.9521K protein precursor [Populus trichocarpa][more]
gi|118484840|gb|ABK94287.1|7.2e-6064.43unknown [Populus trichocarpa][more]
Annotated Terms
The following terms have been associated with this gene:
Vocabulary: INTERPRO
TermDefinition
IPR006501Pectinesterase_inhib_dom
Vocabulary: Molecular Function
TermDefinition
GO:0004857enzyme inhibitor activity
GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0043086 negative regulation of catalytic activity
biological_process GO:0005982 starch metabolic process
biological_process GO:0005985 sucrose metabolic process
biological_process GO:0008150 biological_process
cellular_component GO:0005575 cellular_component
molecular_function GO:0004857 enzyme inhibitor activity
molecular_function GO:0030599 pectinesterase activity
Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CmaCh16G001840.1CmaCh16G001840.1mRNA


InterPro
Analysis Name: InterPro Annotations of Cucurbita maxima
Date Performed: 2017-05-20
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR006501Pectinesterase inhibitor domainGENE3DG3DSA:1.20.140.40coord: 29..189
score: 2.0
IPR006501Pectinesterase inhibitor domainPFAMPF04043PMEIcoord: 30..186
score: 1.1
IPR006501Pectinesterase inhibitor domainSMARTSM00856PMEI_2coord: 27..186
score: 7.8
IPR006501Pectinesterase inhibitor domainTIGRFAMsTIGR01614TIGR01614coord: 1..190
score: 1.8
IPR006501Pectinesterase inhibitor domainunknownSSF101148Plant invertase/pectin methylesterase inhibitorcoord: 29..190
score: 7.85
NoneNo IPR availableunknownCoilCoilcoord: 103..123
scor
NoneNo IPR availablePANTHERPTHR31080FAMILY NOT NAMEDcoord: 6..199
score: 2.7
NoneNo IPR availablePANTHERPTHR31080:SF9INVERTASE/PECTIN METHYLESTERASE INHIBITOR FAMILY PROTEIN / DC 1.2-LIKE PROTEIN-RELATEDcoord: 6..199
score: 2.7