CmoCh04G003470 (gene) Cucurbita moschata (Rifu)

Overview
NameCmoCh04G003470
Typegene
OrganismCucurbita moschata (Cucurbita moschata (Rifu))
DescriptionPlant invertase/pectin methylesterase inhibitor
LocationCmo_Chr04 : 1699344 .. 1699931 (+)
Genome Viewer
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCGAAATCCGCCTTCCTTCTCCCACTCCTCTCTATCTCCGTCCTATTCGCCACCACCAGCGCTGCCGGCTACGGCGGAGCTTCCAATTTCATCAAATCGAGATGCAATTTCACAACATATCCTGACCTATGCGTTCAATCCCTCACAAATTTCGCCTCAAGAATCCAACAAAACCCTCGCCAATTAGTCCAAACAGCTCTAACGGTGAGCCTCTCTCACGCTCGATCCGCCCGATCCTTCGTCTGGAAACTGACGAAGTTCACCGGATTGAAGCCGCGAGAGTACGCGGCGTTGAGAGACTGTCTAGAGGAGGTCGGCGACACGGTGGAACGGCTGAGCAAATCGGTGGAAGAACTGAAGCTGGTTAGAAGATCGAAGAGGAAGGATTTCGAGTGGCACATTAGCAACGTGGAGACCTGGGTGAGTGCGGCGATGACGGACGGTAACACTTGCTCCGATGGATTTGCAGGGGCAGTGCTGAATGGAAGAATTAAATCATCGATTAGGCGTCGCATTGTGGATTTAACTCGATTCATAAGTAATGCCTTGGCTCTGATTAACAACTATGCTCAAAATCAAAGCTAG

mRNA sequence

ATGGCGAAATCCGCCTTCCTTCTCCCACTCCTCTCTATCTCCGTCCTATTCGCCACCACCAGCGCTGCCGGCTACGGCGGAGCTTCCAATTTCATCAAATCGAGATGCAATTTCACAACATATCCTGACCTATGCGTTCAATCCCTCACAAATTTCGCCTCAAGAATCCAACAAAACCCTCGCCAATTAGTCCAAACAGCTCTAACGGTGAGCCTCTCTCACGCTCGATCCGCCCGATCCTTCGTCTGGAAACTGACGAAGTTCACCGGATTGAAGCCGCGAGAGTACGCGGCGTTGAGAGACTGTCTAGAGGAGGTCGGCGACACGGTGGAACGGCTGAGCAAATCGGTGGAAGAACTGAAGCTGGTTAGAAGATCGAAGAGGAAGGATTTCGAGTGGCACATTAGCAACGTGGAGACCTGGGTGAGTGCGGCGATGACGGACGGTAACACTTGCTCCGATGGATTTGCAGGGGCAGTGCTGAATGGAAGAATTAAATCATCGATTAGGCGTCGCATTGTGGATTTAACTCGATTCATAAGTAATGCCTTGGCTCTGATTAACAACTATGCTCAAAATCAAAGCTAG

Coding sequence (CDS)

ATGGCGAAATCCGCCTTCCTTCTCCCACTCCTCTCTATCTCCGTCCTATTCGCCACCACCAGCGCTGCCGGCTACGGCGGAGCTTCCAATTTCATCAAATCGAGATGCAATTTCACAACATATCCTGACCTATGCGTTCAATCCCTCACAAATTTCGCCTCAAGAATCCAACAAAACCCTCGCCAATTAGTCCAAACAGCTCTAACGGTGAGCCTCTCTCACGCTCGATCCGCCCGATCCTTCGTCTGGAAACTGACGAAGTTCACCGGATTGAAGCCGCGAGAGTACGCGGCGTTGAGAGACTGTCTAGAGGAGGTCGGCGACACGGTGGAACGGCTGAGCAAATCGGTGGAAGAACTGAAGCTGGTTAGAAGATCGAAGAGGAAGGATTTCGAGTGGCACATTAGCAACGTGGAGACCTGGGTGAGTGCGGCGATGACGGACGGTAACACTTGCTCCGATGGATTTGCAGGGGCAGTGCTGAATGGAAGAATTAAATCATCGATTAGGCGTCGCATTGTGGATTTAACTCGATTCATAAGTAATGCCTTGGCTCTGATTAACAACTATGCTCAAAATCAAAGCTAG
Homology
[Close]
BLAST of CmoCh04G003470 vs. Swiss-Prot
Match: 21KD_DAUCA (21 kDa protein OS=Daucus carota PE=2 SV=1)

HSP 1 Score: 186.0 bits (471), Expect = 3.8e-46
Identity = 92/181 (50.83%), Postives = 129/181 (71.27%), Query Frame = 1 

Query: 11  LSISVLFATTSAAGYGGASNFIKSRCNFTTYPDLCVQSLTNFASRIQQNPRQLVQTALTV 70
           L  S L    +AA    A+ FIK+ C  TTYP +C QSL+ +A  IQ NP++L  TAL V
Sbjct: 8   LVFSALLVILAAASAAPANQFIKTSCTLTTYPAVCEQSLSAYAKTIQNNPQELASTALQV 67

Query: 71  SLSHARSARSFVWKLTKFTGLKPREYAALRDCLEEVGDTVERLSKSVEELKLVRRSKRKD 130
           SL+  + A++F+ +L KF GLK R+YAA+ DCLEEV D+++R+S+S +E+K +  +K  D
Sbjct: 68  SLTRTQQAQTFMKRLNKFKGLKARQYAAIHDCLEEVEDSLDRVSRSCDEMKNLSHAKGND 127

Query: 131 FEWHISNVETWVSAAMTDGNTCSDGFAGAVLNGRIKSSIRRRIVDLTRFISNALALINNY 190
           F + +SNVETWVSAA+TD  TC DGFAG  ++G+IK S+R ++V + R  SNALAL+NN+
Sbjct: 128 FTFRMSNVETWVSAALTDETTCMDGFAGKGMDGKIKESVRAQVVAVARVTSNALALVNNF 187

Query: 191 A 192
           A
Sbjct: 188 A 188

[Close]
BLAST of CmoCh04G003470 vs. Swiss-Prot
Match: PME1_CITSI (Pectinesterase 1 OS=Citrus sinensis GN=PECS-1.1 PE=2 SV=1)

HSP 1 Score: 91.7 bits (226), Expect = 9.8e-18
Identity = 53/160 (33.12%), Postives = 89/160 (55.62%), Query Frame = 1 

Query: 32  IKSRCNFTTYPDLCVQSLTNF--ASRIQQNPRQLVQTALTVSLSHARSARSFVWKLTKFT 91
           +KS C+ T YPDLC  ++     AS+   + + +++ +L ++ +        + KL K T
Sbjct: 69  LKSSCSSTRYPDLCFSAIAAVPEASKKVTSQKDVIEMSLNITTTAVEHNYFGIQKLLKRT 128

Query: 92  GLKPREYAALRDCLEEVGDTVERLSKSVEELKLVRRSKRKDFEWHISNVETWVSAAMTDG 151
            L  RE  AL DCLE + +T++ L K+VE+L+      +K    H  +++T +SAAMT+ 
Sbjct: 129 NLTKREKVALHDCLETIDETLDELHKAVEDLE--EYPNKKSLSQHADDLKTLMSAAMTNQ 188

Query: 152 NTCSDGFAGAVLNGRIKSSIRRRIVDLTRFISNALALINN 190
            TC DGF+    N  ++ ++    V + +  SNALA+I N
Sbjct: 189 GTCLDGFSHDDANKHVRDALSDGQVHVEKMCSNALAMIKN 226

[Close]
BLAST of CmoCh04G003470 vs. Swiss-Prot
Match: PME3_CITSI (Pectinesterase 3 OS=Citrus sinensis PE=1 SV=1)

HSP 1 Score: 91.7 bits (226), Expect = 9.8e-18
Identity = 53/160 (33.12%), Postives = 89/160 (55.62%), Query Frame = 1 

Query: 32  IKSRCNFTTYPDLCVQSLTNF--ASRIQQNPRQLVQTALTVSLSHARSARSFVWKLTKFT 91
           +KS C+ T YPDLC  ++     AS+   + + +++ +L ++ +        + KL K T
Sbjct: 69  LKSSCSSTRYPDLCFSAIAAVPEASKKVTSQKDVIEMSLNITTTAVEHNYFGIQKLLKRT 128

Query: 92  GLKPREYAALRDCLEEVGDTVERLSKSVEELKLVRRSKRKDFEWHISNVETWVSAAMTDG 151
            L  RE  AL DCLE + +T++ L K+VE+L+      +K    H  +++T +SAAMT+ 
Sbjct: 129 NLTKREKVALHDCLETIDETLDELHKAVEDLE--EYPNKKSLSQHADDLKTLMSAAMTNQ 188

Query: 152 NTCSDGFAGAVLNGRIKSSIRRRIVDLTRFISNALALINN 190
            TC DGF+    N  ++ ++    V + +  SNALA+I N
Sbjct: 189 GTCLDGFSHDDANKHVRDALSDGQVHVEKMCSNALAMIKN 226

[Close]
BLAST of CmoCh04G003470 vs. Swiss-Prot
Match: PME2_ARATH (Pectinesterase 2 OS=Arabidopsis thaliana GN=PME2 PE=2 SV=2)

HSP 1 Score: 86.7 bits (213), Expect = 3.2e-16
Identity = 49/162 (30.25%), Postives = 91/162 (56.17%), Query Frame = 1 

Query: 32  IKSRCNFTTYPDLCVQSLTNFASRIQQNPRQLVQTALTVSLSHARSARSFVWKL-TKFTG 91
           +KS C+ T YP+LC  ++     +   + +++++ +L ++    +     V KL  K  G
Sbjct: 62  LKSVCSSTLYPELCFSAVAATGGKELTSQKEVIEASLNLTTKAVKHNYFAVKKLIAKRKG 121

Query: 92  LKPREYAALRDCLEEVGDTVERLSKSVEELKLVRRSKRKDFEWHISNVETWVSAAMTDGN 151
           L PRE  AL DCLE + +T++ L  +VE+L   +  K+K    H  +++T +S+A+T+  
Sbjct: 122 LTPREVTALHDCLETIDETLDELHVAVEDLH--QYPKQKSLRKHADDLKTLISSAITNQG 181

Query: 152 TCSDGFAGAVLNGRIKSSIRRRIVDLTRFISNALALINNYAQ 193
           TC DGF+    + +++ ++ +  V +    SNALA+I N  +
Sbjct: 182 TCLDGFSYDDADRKVRKALLKGQVHVEHMCSNALAMIKNMTE 221

[Close]
BLAST of CmoCh04G003470 vs. Swiss-Prot
Match: PMEU1_SOLLC (Pectinesterase/pectinesterase inhibitor U1 OS=Solanum lycopersicum GN=PMEU1 PE=2 SV=1)

HSP 1 Score: 84.7 bits (208), Expect = 1.2e-15
Identity = 50/165 (30.30%), Postives = 95/165 (57.58%), Query Frame = 1 

Query: 28  ASNFIKSRCNFTTYPDLCVQSLTNFA--SRIQQNPRQLVQTALTVSLSHARSARSFVWKL 87
           A   +KS C+ T +P+LC  ++ N +  S+   + + +++ +L +++   R     V +L
Sbjct: 62  AHAIVKSACSNTLHPELCYSAIVNVSDFSKKVTSQKDVIELSLNITVKAVRRNYYAVKEL 121

Query: 88  TKFT-GLKPREYAALRDCLEEVGDTVERLSKSVEELKLVRRSKRKDFEWHISNVETWVSA 147
            K   GL PRE  AL DCLE + +T++ L  +VE+L+L     +K  + H+ +++T +S+
Sbjct: 122 IKTRKGLTPREKVALHDCLETMDETLDELHTAVEDLELY--PNKKSLKEHVEDLKTLISS 181

Query: 148 AMTDGNTCSDGFAGAVLNGRIKSSIRRRIVDLTRFISNALALINN 190
           A+T+  TC DGF+    + +++  + +    + +  SNALA+I N
Sbjct: 182 AITNQETCLDGFSHDEADKKVRKVLLKGQKHVEKMCSNALAMICN 224

[Close]
BLAST of CmoCh04G003470 vs. TrEMBL
Match: A0A0A0L8D7_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_3G176260 PE=4 SV=1)

HSP 1 Score: 277.3 bits (708), Expect = 1.4e-71
Identity = 148/195 (75.90%), Postives = 170/195 (87.18%), Query Frame = 1 

Query: 1   MAKSAFLLPLLSISVLFATTSAAGYGGASNFIKSRCNFTTYPDLCVQSLTNFASRIQQNP 60
           MAKS  LL LL +S+L  + SA   GGAS+FIKS+C+  TYPDLCVQSL++F+S IQ+NP
Sbjct: 1   MAKSLSLL-LLLLSILTISASAFN-GGASSFIKSKCSAATYPDLCVQSLSSFSSTIQRNP 60

Query: 61  RQLVQTALTVSLSHARSARSFVWKLTKFTGLKPREYAALRDCLEEVGDTVERLSKSVEEL 120
           RQLVQTAL VSLSHA+S RSFVWKLTKF+GLKPRE AAL+DC+EEVGDTV+RL+KSVEEL
Sbjct: 61  RQLVQTALAVSLSHAQSTRSFVWKLTKFSGLKPRERAALKDCMEEVGDTVDRLNKSVEEL 120

Query: 121 KLVRRSKRKDFEWHISNVETWVSAAMTDGNTCSDGFAGAVLNGRIKSSIRRRIVDLTRFI 180
           K V  SK KDF+WHISNVETWVSAAMTD NTCSDGFAG+ LNGRIKSS+R RIVD+TR I
Sbjct: 121 KRVSGSK-KDFQWHISNVETWVSAAMTDENTCSDGFAGSALNGRIKSSVRGRIVDVTRVI 180

Query: 181 SNALALINNYAQNQS 196
           SNAL+LIN YA+NQS
Sbjct: 181 SNALSLINKYAENQS 192

[Close]
BLAST of CmoCh04G003470 vs. TrEMBL
Match: B9R9Q7_RICCO (21 kDa protein, putative OS=Ricinus communis GN=RCOM_1500010 PE=4 SV=1)

HSP 1 Score: 229.6 bits (584), Expect = 3.4e-57
Identity = 119/193 (61.66%), Postives = 149/193 (77.20%), Query Frame = 1 

Query: 1   MAKSAFLLPLLSISVLFATT--SAAGYGGASNFIKSRCNFTTYPDLCVQSLTNFASRIQQ 60
           M K   LL L+S+ +  ++T  SAA    ASNFIK+ C  TTYP LC+QSL+ +A+ IQQ
Sbjct: 1   MVKPTLLLLLVSVLLYISSTATSAAAPAAASNFIKASCRTTTYPALCIQSLSAYATSIQQ 60

Query: 61  NPRQLVQTALTVSLSHARSARSFVWKLTKFTGLKPREYAALRDCLEEVGDTVERLSKSVE 120
           NP QL QTAL+VSL +A+S R+FV+KLTKF G+KPRE AAL+DCLEE+ DT +RLSKS  
Sbjct: 61  NPLQLTQTALSVSLENAQSTRTFVYKLTKFKGVKPREMAALKDCLEEIDDTADRLSKSYN 120

Query: 121 ELKLVRRSKRKDFEWHISNVETWVSAAMTDGNTCSDGFAGAVLNGRIKSSIRRRIVDLTR 180
           ELK +   K KDF+WH+SNVETWVSAA+TD NTC+DGFAG  LNG++KSSI+ RIV + +
Sbjct: 121 ELKNLGHYKGKDFQWHMSNVETWVSAALTDENTCTDGFAGKALNGKMKSSIKARIVKVAQ 180

Query: 181 FISNALALINNYA 192
             SNAL+LIN YA
Sbjct: 181 VTSNALSLINKYA 193

[Close]
BLAST of CmoCh04G003470 vs. TrEMBL
Match: A9PDL3_POPTR (21K protein OS=Populus trichocarpa GN=POPTR_0012s14560g PE=2 SV=1)

HSP 1 Score: 221.1 bits (562), Expect = 1.2e-54
Identity = 115/194 (59.28%), Postives = 146/194 (75.26%), Query Frame = 1 

Query: 1   MAKSAFLLPLLSISVLFATTSAAGY----GGASNFIKSRCNFTTYPDLCVQSLTNFASRI 60
           MAKS+ +L LLSI  +  T ++A        A+NFIK+ C  TTYP LCVQSL+ +A+ I
Sbjct: 1   MAKSSLVLLLLSILYIAGTATSASTTSSSASATNFIKASCTATTYPALCVQSLSLYATSI 60

Query: 61  QQNPRQLVQTALTVSLSHARSARSFVWKLTKFTGLKPREYAALRDCLEEVGDTVERLSKS 120
            Q+PRQL+QTAL VSL  A+S ++FV+KLTKF G+KPRE AA++DC EE+ DTV+RL KS
Sbjct: 61  NQSPRQLIQTALAVSLDKAQSTKTFVYKLTKFKGVKPREKAAIKDCFEEIDDTVDRLVKS 120

Query: 121 VEELKLVRRSKRKDFEWHISNVETWVSAAMTDGNTCSDGFAGAVLNGRIKSSIRRRIVDL 180
           V+ELK +  SK  DF+WHISNV+TW+SA +TD NTC DGFAG  LNGRIK+SI+ R V +
Sbjct: 121 VKELKNMGSSKGSDFQWHISNVQTWISAGLTDENTCVDGFAGKALNGRIKNSIKARFVHV 180

Query: 181 TRFISNALALINNY 191
            R  SNALALIN +
Sbjct: 181 ERVTSNALALINKF 194

[Close]
BLAST of CmoCh04G003470 vs. TrEMBL
Match: M5X6V6_PRUPE (Uncharacterized protein OS=Prunus persica GN=PRUPE_ppa011831mg PE=4 SV=1)

HSP 1 Score: 220.7 bits (561), Expect = 1.6e-54
Identity = 109/191 (57.07%), Postives = 149/191 (78.01%), Query Frame = 1 

Query: 1   MAKSAFLLPLLSISVLFATTSAAGYGGASNFIKSRCNFTTYPDLCVQSLTNFASRIQQNP 60
           MA+++F L LL I     T ++      +NFIKS C+ TTYP +C+QSL+++A+ IQQ+P
Sbjct: 1   MARASFFLLLLPIFYFVGTANSTATTSDTNFIKSSCSATTYPQVCIQSLSSYATSIQQSP 60

Query: 61  RQLVQTALTVSLSHARSARSFVWKLTKFTGLKPREYAALRDCLEEVGDTVERLSKSVEEL 120
           RQL Q AL+VSL+ A+ A++FV KL KF GLK REY A++DCLEE+GDTV+RLSKSV+EL
Sbjct: 61  RQLAQAALSVSLTRAQKAKTFVTKLAKFKGLKAREYGAVKDCLEEMGDTVDRLSKSVQEL 120

Query: 121 KLVRRSKRKDFEWHISNVETWVSAAMTDGNTCSDGFAGAVLNGRIKSSIRRRIVDLTRFI 180
           K + +SK +DF WH+SNVETWVSAA+TD NTC DGF+G  L+G+IK+SIR +++++ +  
Sbjct: 121 KNMGKSKGQDFVWHMSNVETWVSAALTDDNTCLDGFSGKALDGKIKASIRAQVLNVAQCT 180

Query: 181 SNALALINNYA 192
           SNALAL N +A
Sbjct: 181 SNALALCNRFA 191

[Close]
BLAST of CmoCh04G003470 vs. TrEMBL
Match: A9PD34_POPTR (Putative uncharacterized protein OS=Populus trichocarpa PE=2 SV=1)

HSP 1 Score: 218.8 bits (556), Expect = 5.9e-54
Identity = 114/194 (58.76%), Postives = 145/194 (74.74%), Query Frame = 1 

Query: 1   MAKSAFLLPLLSISVLFATTSAAGY----GGASNFIKSRCNFTTYPDLCVQSLTNFASRI 60
           MAKS+ +L LLSI  +  T ++A        A+NFIK+ C  TTYP LCVQSL+ +A+ I
Sbjct: 1   MAKSSLVLLLLSILYIAGTATSASTTSSSASATNFIKASCTATTYPALCVQSLSLYATSI 60

Query: 61  QQNPRQLVQTALTVSLSHARSARSFVWKLTKFTGLKPREYAALRDCLEEVGDTVERLSKS 120
            Q+PRQL+QTAL VSL  A+S ++FV+KLTKF G+KPRE AA++DC EE+ DTV+RL KS
Sbjct: 61  NQSPRQLIQTALAVSLDKAQSTKTFVYKLTKFKGVKPREKAAIKDCFEEIDDTVDRLVKS 120

Query: 121 VEELKLVRRSKRKDFEWHISNVETWVSAAMTDGNTCSDGFAGAVLNGRIKSSIRRRIVDL 180
           V+ELK +  SK  DF+WHISNV+TW+SA +TD  TC DGFAG  LNGRIK+SI+ R V +
Sbjct: 121 VKELKNMGSSKGSDFQWHISNVQTWISAGLTDEKTCVDGFAGKALNGRIKNSIKARFVHV 180

Query: 181 TRFISNALALINNY 191
            R  SNALALIN +
Sbjct: 181 ERVTSNALALINKF 194

[Close]
BLAST of CmoCh04G003470 vs. TAIR10
Match: AT3G47380.1 (AT3G47380.1 Plant invertase/pectin methylesterase inhibitor superfamily protein)

HSP 1 Score: 187.6 bits (475), Expect = 7.4e-48
Identity = 100/200 (50.00%), Postives = 143/200 (71.50%), Query Frame = 1 

Query: 1   MAKSAF---LLPLLSISVLFATTSAAGYGGAS----NFIKSRCNFTTYPDLCVQSLTNFA 60
           MAK  F    L LLS ++L A++SA      S    NFI++ C  TTYP +CV SLT +A
Sbjct: 1   MAKQIFYTLFLFLLSTAILTASSSAPRAAITSKRAINFIQASCKATTYPTVCVNSLTGYA 60

Query: 61  SRIQQNPRQLVQTALTVSLSHARSARSFVWKLTKFTGLKPREYAALRDCLEEVGDTVERL 120
           + IQ +PR+L +TAL V+++ A+S + FVW+L +FT LK RE  A++DC+EE+ D V+RL
Sbjct: 61  NSIQTSPRRLAETALNVTVTQAQSTKVFVWRLGRFTSLKKREIQAVKDCIEEIHDAVDRL 120

Query: 121 SKSVEELKLVRRSKRKDFEW-HISNVETWVSAAMTDGNTCSDGFAGAVLNGRIKSSIRRR 180
           + S+ E+K+   +K +D  W H+SN +TW SAA+T+ NTCSDGFAG V++GR+K+S+R R
Sbjct: 121 TMSIHEVKMCGSAKGRDQFWFHMSNAQTWTSAALTNANTCSDGFAGRVMDGRVKNSVRAR 180

Query: 181 IVDLTRFISNALALINNYAQ 193
           I++L R  SNALALIN +A+
Sbjct: 181 ILNLGRGTSNALALINAFAK 200

[Close]
BLAST of CmoCh04G003470 vs. TAIR10
Match: AT5G62350.1 (AT5G62350.1 Plant invertase/pectin methylesterase inhibitor superfamily protein)

HSP 1 Score: 187.6 bits (475), Expect = 7.4e-48
Identity = 105/201 (52.24%), Postives = 146/201 (72.64%), Query Frame = 1 

Query: 1   MAKSAFLLPLLSISVLFA----TTSAAGYGGAS----NFIKSRCNFTTYPDLCVQSLTNF 60
           MAK  +L  LLSIS L +      +AA   GAS    NFI+S C  TTYP LCV SL+ +
Sbjct: 1   MAKQ-YLFVLLSISYLLSLELTAATAASQTGASKKAINFIQSSCKTTTYPALCVHSLSVY 60

Query: 61  ASRIQQNPRQLVQTALTVSLSHARSARSFVWKLTKFTGLKPREYAALRDCLEEVGDTVER 120
           A+ IQ +P++L +TA+ V+LS A+S + FV +LT+  GLK RE  A++DC+EE+ DTV+R
Sbjct: 61  ANDIQTSPKRLAETAIAVTLSRAQSTKLFVSRLTRMKGLKKREVEAIKDCVEEMNDTVDR 120

Query: 121 LSKSVEELKLVRRSKRKD-FEWHISNVETWVSAAMTDGNTCSDGFAGAVLNGRIKSSIRR 180
           L+KSV+ELKL   +K +D F +H+SN +TW SAA+TD NTCSDGF+G V++GRIK+S+R 
Sbjct: 121 LTKSVQELKLCGSAKDQDQFAYHMSNAQTWTSAALTDENTCSDGFSGRVMDGRIKNSVRA 180

Query: 181 RIVDLTRFISNALALINNYAQ 193
           RI+++    SNAL+LIN +A+
Sbjct: 181 RIMNVGHETSNALSLINAFAK 200

[Close]
BLAST of CmoCh04G003470 vs. TAIR10
Match: AT4G12390.1 (AT4G12390.1 pectin methylesterase inhibitor 1)

HSP 1 Score: 172.2 bits (435), Expect = 3.2e-43
Identity = 93/186 (50.00%), Postives = 124/186 (66.67%), Query Frame = 1 

Query: 8   LPLLSISVLFATTSAAGYGGASNFIKSRCNFTTYPDLCVQSLTNFASRIQQNPRQLVQTA 67
           LPLL  S +   +S+     + NFI S C  T Y  LCV+ L  FA +I++N  QL QTA
Sbjct: 16  LPLLCQSTIAKPSSSPNPSSSINFIVSSCRVTRYQTLCVKCLAAFADKIRRNENQLAQTA 75

Query: 68  LTVSLSHARSARSFVWKLTKFTGLKPREYAALRDCLEEVGDTVERLSKSVEELKLVRRS- 127
           L V+L   +S   +V KLTK   +K REY A++DC+E +GD +E L++S+ ELK V RS 
Sbjct: 76  LAVTLVRVQSTTIYVGKLTKARRIKRREYLAVKDCVENLGDGLEMLAQSMRELKQVGRSG 135

Query: 128 -KRKDFEWHISNVETWVSAAMTDGNTCSDGFAGAVLNGRIKSSIRRRIVDLTRFISNALA 187
             R +F W +SNVETWVSAA+TD  TC DGF G V++G +KS+IRRR+V + R  SNALA
Sbjct: 136 RDRDEFLWRLSNVETWVSAALTDETTCLDGFDGKVMDGVVKSAIRRRVVHVARVTSNALA 195

Query: 188 LINNYA 192
           L+N +A
Sbjct: 196 LVNRFA 201

[Close]
BLAST of CmoCh04G003470 vs. TAIR10
Match: AT4G25260.1 (AT4G25260.1 Plant invertase/pectin methylesterase inhibitor superfamily protein)

HSP 1 Score: 166.0 bits (419), Expect = 2.3e-41
Identity = 86/187 (45.99%), Postives = 127/187 (67.91%), Query Frame = 1 

Query: 6   FLLPLLSISVLFATTSAAGYGGASNFIKSRCNFTTYPDLCVQSLTNFASRIQQNPRQLVQ 65
           F+L L + +++ A        G + FIK+ C  T+YPD C QSL+++AS I++ PR+L +
Sbjct: 12  FVLYLSTAAIVMARNLEEESSGDTEFIKASCETTSYPDRCFQSLSSYASEIKKQPRKLAE 71

Query: 66  TALTVSLSHARSARSFVWKLTKFTGLKPREYAALRDCLEEVGDTVERLSKSVEELK-LVR 125
           TAL VS++ A+SA+++V ++T + G+  R++ A+ DCLEE+GDTV+RLS S++ELK L  
Sbjct: 72  TALAVSIARAKSAKTYVSEMTDYKGITKRQHEAVADCLEEMGDTVDRLSNSLKELKHLEE 131

Query: 126 RSKRKDFEWHISNVETWVSAAMTDGNTCSDGFAGAVLNGRIKSSIRRRIVDLTRFISNAL 185
               +DF + +SNV TW SAA+TD   C DGF G  + G +KS IR  IV +    SNAL
Sbjct: 132 GDSGEDFWFCLSNVRTWTSAALTDETACMDGFGGKAMAGELKSLIRTHIVSVAEETSNAL 191

Query: 186 ALINNYA 192
           ALIN++A
Sbjct: 192 ALINDFA 198

[Close]
BLAST of CmoCh04G003470 vs. TAIR10
Match: AT1G62770.1 (AT1G62770.1 Plant invertase/pectin methylesterase inhibitor superfamily protein)

HSP 1 Score: 158.3 bits (399), Expect = 4.8e-39
Identity = 87/198 (43.94%), Postives = 133/198 (67.17%), Query Frame = 1 

Query: 1   MAKSAFLLPLLSISVLFATTSAAGYGGASNFIKSRCNFTTYPDLCVQSLTNFASRIQQNP 60
           +  + FL+ LLSI++L ++++      +  FI S C  T YP LCV +L+ +A++I+ N 
Sbjct: 3   LKNTIFLVILLSITILQSSSATPNRSESDQFIVSSCQTTQYPSLCVHTLSAYATKIRHNN 62

Query: 61  RQ-LVQTALTVSLSHARSARSFVWKLTKFTG-LKPREYAALRDCLEEVGDTVERLSKSVE 120
            Q L QTAL +SL+ A+S   FV KLTK T   K REY A++DC+E +G++V+RL++SV+
Sbjct: 63  DQDLAQTALIISLARAKSVTIFVAKLTKETPKFKRREYLAIKDCIEVLGNSVDRLAQSVK 122

Query: 121 ELKLVRRS-KRKDFEWHISNVETWVSAAMTDGNTCSDGFAGAVLNGRIKSSIRRRIVDLT 180
           EL     +   +DF W +SNV+TWVSAA+TD  TC DGF+   + G++K  IR ++V + 
Sbjct: 123 ELARAGHAVASEDFMWKMSNVQTWVSAALTDETTCLDGFSERAMGGKVKRLIRYKVVHVA 182

Query: 181 RFISNALALINNYAQNQS 196
           +  SNALAL+N +A+ +S
Sbjct: 183 QVTSNALALVNQFAEKRS 200

[Close]
BLAST of CmoCh04G003470 vs. NCBI nr
Match: gi|659077241|ref|XP_008439100.1| (PREDICTED: 21 kDa protein-like [Cucumis melo])

HSP 1 Score: 278.5 bits (711), Expect = 9.0e-72
Identity = 148/195 (75.90%), Postives = 168/195 (86.15%), Query Frame = 1 

Query: 1   MAKSAFLLPLLSISVLFATTSAAGYGGASNFIKSRCNFTTYPDLCVQSLTNFASRIQQNP 60
           MAKS  LL LL +S+L  + SA   GGASNFIKS+C+  TYPDLCVQSL++FAS IQ+NP
Sbjct: 1   MAKSLSLL-LLLLSILTISASAFN-GGASNFIKSKCSAATYPDLCVQSLSSFASTIQRNP 60

Query: 61  RQLVQTALTVSLSHARSARSFVWKLTKFTGLKPREYAALRDCLEEVGDTVERLSKSVEEL 120
           RQLVQTAL VSLS A+S RSFVWKLTKF+GLKPRE AAL+DC+EEVGDTV+RL+KSVEEL
Sbjct: 61  RQLVQTALAVSLSRAQSTRSFVWKLTKFSGLKPRERAALKDCMEEVGDTVDRLNKSVEEL 120

Query: 121 KLVRRSKRKDFEWHISNVETWVSAAMTDGNTCSDGFAGAVLNGRIKSSIRRRIVDLTRFI 180
           K V  SK+KDF WHISNVETWVSAAMTD NTCSDGFAG+ LNGRIKSS+R RIVD+TR I
Sbjct: 121 KRVSGSKKKDFLWHISNVETWVSAAMTDENTCSDGFAGSALNGRIKSSVRGRIVDVTRVI 180

Query: 181 SNALALINNYAQNQS 196
           SNAL+LIN YA+ QS
Sbjct: 181 SNALSLINKYAETQS 193

[Close]
BLAST of CmoCh04G003470 vs. NCBI nr
Match: gi|449460808|ref|XP_004148136.1| (PREDICTED: 21 kDa protein-like [Cucumis sativus])

HSP 1 Score: 277.3 bits (708), Expect = 2.0e-71
Identity = 148/195 (75.90%), Postives = 170/195 (87.18%), Query Frame = 1 

Query: 1   MAKSAFLLPLLSISVLFATTSAAGYGGASNFIKSRCNFTTYPDLCVQSLTNFASRIQQNP 60
           MAKS  LL LL +S+L  + SA   GGAS+FIKS+C+  TYPDLCVQSL++F+S IQ+NP
Sbjct: 1   MAKSLSLL-LLLLSILTISASAFN-GGASSFIKSKCSAATYPDLCVQSLSSFSSTIQRNP 60

Query: 61  RQLVQTALTVSLSHARSARSFVWKLTKFTGLKPREYAALRDCLEEVGDTVERLSKSVEEL 120
           RQLVQTAL VSLSHA+S RSFVWKLTKF+GLKPRE AAL+DC+EEVGDTV+RL+KSVEEL
Sbjct: 61  RQLVQTALAVSLSHAQSTRSFVWKLTKFSGLKPRERAALKDCMEEVGDTVDRLNKSVEEL 120

Query: 121 KLVRRSKRKDFEWHISNVETWVSAAMTDGNTCSDGFAGAVLNGRIKSSIRRRIVDLTRFI 180
           K V  SK KDF+WHISNVETWVSAAMTD NTCSDGFAG+ LNGRIKSS+R RIVD+TR I
Sbjct: 121 KRVSGSK-KDFQWHISNVETWVSAAMTDENTCSDGFAGSALNGRIKSSVRGRIVDVTRVI 180

Query: 181 SNALALINNYAQNQS 196
           SNAL+LIN YA+NQS
Sbjct: 181 SNALSLINKYAENQS 192

[Close]
BLAST of CmoCh04G003470 vs. NCBI nr
Match: gi|255539735|ref|XP_002510932.1| (PREDICTED: 21 kDa protein [Ricinus communis])

HSP 1 Score: 229.6 bits (584), Expect = 4.8e-57
Identity = 119/193 (61.66%), Postives = 149/193 (77.20%), Query Frame = 1 

Query: 1   MAKSAFLLPLLSISVLFATT--SAAGYGGASNFIKSRCNFTTYPDLCVQSLTNFASRIQQ 60
           M K   LL L+S+ +  ++T  SAA    ASNFIK+ C  TTYP LC+QSL+ +A+ IQQ
Sbjct: 1   MVKPTLLLLLVSVLLYISSTATSAAAPAAASNFIKASCRTTTYPALCIQSLSAYATSIQQ 60

Query: 61  NPRQLVQTALTVSLSHARSARSFVWKLTKFTGLKPREYAALRDCLEEVGDTVERLSKSVE 120
           NP QL QTAL+VSL +A+S R+FV+KLTKF G+KPRE AAL+DCLEE+ DT +RLSKS  
Sbjct: 61  NPLQLTQTALSVSLENAQSTRTFVYKLTKFKGVKPREMAALKDCLEEIDDTADRLSKSYN 120

Query: 121 ELKLVRRSKRKDFEWHISNVETWVSAAMTDGNTCSDGFAGAVLNGRIKSSIRRRIVDLTR 180
           ELK +   K KDF+WH+SNVETWVSAA+TD NTC+DGFAG  LNG++KSSI+ RIV + +
Sbjct: 121 ELKNLGHYKGKDFQWHMSNVETWVSAALTDENTCTDGFAGKALNGKMKSSIKARIVKVAQ 180

Query: 181 FISNALALINNYA 192
             SNAL+LIN YA
Sbjct: 181 VTSNALSLINKYA 193

[Close]
BLAST of CmoCh04G003470 vs. NCBI nr
Match: gi|224120250|ref|XP_002318283.1| (21K protein precursor [Populus trichocarpa])

HSP 1 Score: 221.1 bits (562), Expect = 1.7e-54
Identity = 115/194 (59.28%), Postives = 146/194 (75.26%), Query Frame = 1 

Query: 1   MAKSAFLLPLLSISVLFATTSAAGY----GGASNFIKSRCNFTTYPDLCVQSLTNFASRI 60
           MAKS+ +L LLSI  +  T ++A        A+NFIK+ C  TTYP LCVQSL+ +A+ I
Sbjct: 1   MAKSSLVLLLLSILYIAGTATSASTTSSSASATNFIKASCTATTYPALCVQSLSLYATSI 60

Query: 61  QQNPRQLVQTALTVSLSHARSARSFVWKLTKFTGLKPREYAALRDCLEEVGDTVERLSKS 120
            Q+PRQL+QTAL VSL  A+S ++FV+KLTKF G+KPRE AA++DC EE+ DTV+RL KS
Sbjct: 61  NQSPRQLIQTALAVSLDKAQSTKTFVYKLTKFKGVKPREKAAIKDCFEEIDDTVDRLVKS 120

Query: 121 VEELKLVRRSKRKDFEWHISNVETWVSAAMTDGNTCSDGFAGAVLNGRIKSSIRRRIVDL 180
           V+ELK +  SK  DF+WHISNV+TW+SA +TD NTC DGFAG  LNGRIK+SI+ R V +
Sbjct: 121 VKELKNMGSSKGSDFQWHISNVQTWISAGLTDENTCVDGFAGKALNGRIKNSIKARFVHV 180

Query: 181 TRFISNALALINNY 191
            R  SNALALIN +
Sbjct: 181 ERVTSNALALINKF 194

[Close]
BLAST of CmoCh04G003470 vs. NCBI nr
Match: gi|596049257|ref|XP_007220475.1| (hypothetical protein PRUPE_ppa011831mg [Prunus persica])

HSP 1 Score: 220.7 bits (561), Expect = 2.2e-54
Identity = 109/191 (57.07%), Postives = 149/191 (78.01%), Query Frame = 1 

Query: 1   MAKSAFLLPLLSISVLFATTSAAGYGGASNFIKSRCNFTTYPDLCVQSLTNFASRIQQNP 60
           MA+++F L LL I     T ++      +NFIKS C+ TTYP +C+QSL+++A+ IQQ+P
Sbjct: 1   MARASFFLLLLPIFYFVGTANSTATTSDTNFIKSSCSATTYPQVCIQSLSSYATSIQQSP 60

Query: 61  RQLVQTALTVSLSHARSARSFVWKLTKFTGLKPREYAALRDCLEEVGDTVERLSKSVEEL 120
           RQL Q AL+VSL+ A+ A++FV KL KF GLK REY A++DCLEE+GDTV+RLSKSV+EL
Sbjct: 61  RQLAQAALSVSLTRAQKAKTFVTKLAKFKGLKAREYGAVKDCLEEMGDTVDRLSKSVQEL 120

Query: 121 KLVRRSKRKDFEWHISNVETWVSAAMTDGNTCSDGFAGAVLNGRIKSSIRRRIVDLTRFI 180
           K + +SK +DF WH+SNVETWVSAA+TD NTC DGF+G  L+G+IK+SIR +++++ +  
Sbjct: 121 KNMGKSKGQDFVWHMSNVETWVSAALTDDNTCLDGFSGKALDGKIKASIRAQVLNVAQCT 180

Query: 181 SNALALINNYA 192
           SNALAL N +A
Sbjct: 181 SNALALCNRFA 191

The following BLAST results are available for this feature:
BLAST of CmoCh04G003470 vs. Swiss-Prot
Match NameE-valueIdentityDescription
21KD_DAUCA3.8e-4650.8321 kDa protein OS=Daucus carota PE=2 SV=1[more]
PME1_CITSI9.8e-1833.13Pectinesterase 1 OS=Citrus sinensis GN=PECS-1.1 PE=2 SV=1[more]
PME3_CITSI9.8e-1833.13Pectinesterase 3 OS=Citrus sinensis PE=1 SV=1[more]
PME2_ARATH3.2e-1630.25Pectinesterase 2 OS=Arabidopsis thaliana GN=PME2 PE=2 SV=2[more]
PMEU1_SOLLC1.2e-1530.30Pectinesterase/pectinesterase inhibitor U1 OS=Solanum lycopersicum GN=PMEU1 PE=2... [more]
BLAST of CmoCh04G003470 vs. TrEMBL
Match NameE-valueIdentityDescription
A0A0A0L8D7_CUCSA1.4e-7175.90Uncharacterized protein OS=Cucumis sativus GN=Csa_3G176260 PE=4 SV=1[more]
B9R9Q7_RICCO3.4e-5761.6621 kDa protein, putative OS=Ricinus communis GN=RCOM_1500010 PE=4 SV=1[more]
A9PDL3_POPTR1.2e-5459.2821K protein OS=Populus trichocarpa GN=POPTR_0012s14560g PE=2 SV=1[more]
M5X6V6_PRUPE1.6e-5457.07Uncharacterized protein OS=Prunus persica GN=PRUPE_ppa011831mg PE=4 SV=1[more]
A9PD34_POPTR5.9e-5458.76Putative uncharacterized protein OS=Populus trichocarpa PE=2 SV=1[more]
BLAST of CmoCh04G003470 vs. TAIR10
Match NameE-valueIdentityDescription
AT3G47380.17.4e-4850.00 Plant invertase/pectin methylesterase inhibitor superfamily protein[more]
AT5G62350.17.4e-4852.24 Plant invertase/pectin methylesterase inhibitor superfamily protein[more]
AT4G12390.13.2e-4350.00 pectin methylesterase inhibitor 1[more]
AT4G25260.12.3e-4145.99 Plant invertase/pectin methylesterase inhibitor superfamily protein[more]
AT1G62770.14.8e-3943.94 Plant invertase/pectin methylesterase inhibitor superfamily protein[more]
BLAST of CmoCh04G003470 vs. NCBI nr
Match NameE-valueIdentityDescription
gi|659077241|ref|XP_008439100.1|9.0e-7275.90PREDICTED: 21 kDa protein-like [Cucumis melo][more]
gi|449460808|ref|XP_004148136.1|2.0e-7175.90PREDICTED: 21 kDa protein-like [Cucumis sativus][more]
gi|255539735|ref|XP_002510932.1|4.8e-5761.66PREDICTED: 21 kDa protein [Ricinus communis][more]
gi|224120250|ref|XP_002318283.1|1.7e-5459.2821K protein precursor [Populus trichocarpa][more]
gi|596049257|ref|XP_007220475.1|2.2e-5457.07hypothetical protein PRUPE_ppa011831mg [Prunus persica][more]
Annotated Terms
The following terms have been associated with this gene:
Vocabulary: INTERPRO
TermDefinition
IPR006501Pectinesterase_inhib_dom
Vocabulary: Molecular Function
TermDefinition
GO:0004857enzyme inhibitor activity
GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0043086 negative regulation of catalytic activity
biological_process GO:0005982 starch metabolic process
biological_process GO:0005985 sucrose metabolic process
biological_process GO:0008150 biological_process
cellular_component GO:0005575 cellular_component
molecular_function GO:0004857 enzyme inhibitor activity
molecular_function GO:0030599 pectinesterase activity
Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CmoCh04G003470.1CmoCh04G003470.1mRNA


InterPro
Analysis Name: InterPro Annotations of Cucurbita moschata
Date Performed: 2017-05-19
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR006501Pectinesterase inhibitor domainGENE3DG3DSA:1.20.140.40coord: 29..189
score: 1.8
IPR006501Pectinesterase inhibitor domainPFAMPF04043PMEIcoord: 30..186
score: 2.9
IPR006501Pectinesterase inhibitor domainSMARTSM00856PMEI_2coord: 27..186
score: 3.4
IPR006501Pectinesterase inhibitor domainTIGRFAMsTIGR01614TIGR01614coord: 1..190
score: 1.5
IPR006501Pectinesterase inhibitor domainunknownSSF101148Plant invertase/pectin methylesterase inhibitorcoord: 29..190
score: 7.19
NoneNo IPR availablePANTHERPTHR31080FAMILY NOT NAMEDcoord: 6..192
score: 2.3
NoneNo IPR availablePANTHERPTHR31080:SF9INVERTASE/PECTIN METHYLESTERASE INHIBITOR FAMILY PROTEIN / DC 1.2-LIKE PROTEIN-RELATEDcoord: 6..192
score: 2.3