CSPI03G16000 (gene) Wild cucumber (PI 183967) | Cucurbit Genomics Database (CuGenDB)

Overview


Name	CSPI03G16000
Type	gene
Organism	Cucumis sativus (Wild cucumber (PI 183967))
Description	Plant invertase/pectin methylesterase inhibitor superfamily protein
Location	Chr3 : 11985725 .. 11986693 (-)

Genome Viewer

Gene Expression

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

CCTTCCTCCTAAATTCCTTCCATTTGCATAAAAAAAGAAAAGAAAAGAAAACAGAGATGGGTATCTCAAACTCCAAGAAAAGTTTGATTCTTTTCCAATTAATTCTCCAATTAACACTAATTCATTCAGCCATAACTCCACAAAGCAGCACAGAGTTCATAAAAAGCTCTTGCAGTTCCACCACTTACCCTCGTCTCTGTTTTTCATCTCTTTCAGTCCATGCCAATGCCATCCAAACAAGTCCCCGCCTTTTGGCCACTGCCGCCCTCTCCGTTTCCCTCTCCTCCGTCAAGTCCACCGCCACTCAAATCCTCAAGCTTTCTCACTCCCATGGCCTCCCCTCCAGAGATGTCTCTGCTCTTGACGACTGTTTGGAAGAACTGAGTGACTCTGTCGACTCCCTCGCTGCTTCCATTTCCGAGATGCCGAAGCTCCGTGGAACCAATTTCGACCTCGCAATGAGTAATGTTCAGACATGGGTTAGCGCCGCTCTGACGGATGAAACCACATGTAGCGAAGGGTTTCAGGGGAAGACGGTGAACGGAGGCGTTAAGGGTGTGGTTAGGACTAAGATTGTCAACATTGCGCAATTGACTAGTAACGCTCTGTCTTTGATTAATCAAATTGGTGATCTGCATTAGCGACGATGGGATGTGGGTTGGTGTGTACTATATTTTATTGGTGTGAGATACAAAAGTTGGAATCGTACGTATGATATTTAGGTTAGAAGGTGGAGTCTTTGTCATAGTATTGTATACTTAGTAATATATAGGTTTAAATAGGGTTTATGTGTGGAGTTTGGAGTTATTTTTGCATTATTAATTGGATTAATTAGGATTTGTGGGGAGATGATTTCTGTTTACTATTCACTAAACACGGTAAAAAAAGAACATATTTGTAAAAGTAAAGGTATAGTGAGATGAAATGGAGTATGGTATTCTTAAAATACATCCCTAAAATACCTTTGAT

mRNA sequence

ATGGGTATCTCAAACTCCAAGAAAAGTTTGATTCTTTTCCAATTAATTCTCCAATTAACACTAATTCATTCAGCCATAACTCCACAAAGCAGCACAGAGTTCATAAAAAGCTCTTGCAGTTCCACCACTTACCCTCGTCTCTGTTTTTCATCTCTTTCAGTCCATGCCAATGCCATCCAAACAAGTCCCCGCCTTTTGGCCACTGCCGCCCTCTCCGTTTCCCTCTCCTCCGTCAAGTCCACCGCCACTCAAATCCTCAAGCTTTCTCACTCCCATGGCCTCCCCTCCAGAGATGTCTCTGCTCTTGACGACTGTTTGGAAGAACTGAGTGACTCTGTCGACTCCCTCGCTGCTTCCATTTCCGAGATGCCGAAGCTCCGTGGAACCAATTTCGACCTCGCAATGAGTAATGTTCAGACATGGGTTAGCGCCGCTCTGACGGATGAAACCACATGTAGCGAAGGGTTTCAGGGGAAGACGGTGAACGGAGGCGTTAAGGGTGTGGTTAGGACTAAGATTGTCAACATTGCGCAATTGACTAGTAACGCTCTGTCTTTGATTAATCAAATTGGTGATCTGCATTAG

Coding sequence (CDS)

ATGGGTATCTCAAACTCCAAGAAAAGTTTGATTCTTTTCCAATTAATTCTCCAATTAACACTAATTCATTCAGCCATAACTCCACAAAGCAGCACAGAGTTCATAAAAAGCTCTTGCAGTTCCACCACTTACCCTCGTCTCTGTTTTTCATCTCTTTCAGTCCATGCCAATGCCATCCAAACAAGTCCCCGCCTTTTGGCCACTGCCGCCCTCTCCGTTTCCCTCTCCTCCGTCAAGTCCACCGCCACTCAAATCCTCAAGCTTTCTCACTCCCATGGCCTCCCCTCCAGAGATGTCTCTGCTCTTGACGACTGTTTGGAAGAACTGAGTGACTCTGTCGACTCCCTCGCTGCTTCCATTTCCGAGATGCCGAAGCTCCGTGGAACCAATTTCGACCTCGCAATGAGTAATGTTCAGACATGGGTTAGCGCCGCTCTGACGGATGAAACCACATGTAGCGAAGGGTTTCAGGGGAAGACGGTGAACGGAGGCGTTAAGGGTGTGGTTAGGACTAAGATTGTCAACATTGCGCAATTGACTAGTAACGCTCTGTCTTTGATTAATCAAATTGGTGATCTGCATTAG

Homology

[Close]

BLAST of CSPI03G16000 vs. Swiss-Prot
Match: 21KD_DAUCA (21 kDa protein OS=Daucus carota PE=2 SV=1)

HSP 1 Score: 150.6 bits (379), Expect = 1.8e-35
Identity = 85/190 (44.74%), Postives = 126/190 (66.32%), Query Frame = 1


Query: 8   KSLILFQLILQLTLIHSAITPQSSTEFIKSSCSSTTYPRLCFSSLSVHANAIQTSPRLLA 67
           KS ++F  +L   +I +A +   + +FIK+SC+ TTYP +C  SLS +A  IQ +P+ LA
Sbjct: 5   KSTLVFSALL---VILAAASAAPANQFIKTSCTLTTYPAVCEQSLSAYAKTIQNNPQELA 64

Query: 68  TAALSVSLSSVKSTATQILKLSHSHGLPSRDVSALDDCLEELSDSVDSLAASISEMPKL- 127
           + AL VSL+  +   T + +L+   GL +R  +A+ DCLEE+ DS+D ++ S  EM  L 
Sbjct: 65  STALQVSLTRTQQAQTFMKRLNKFKGLKARQYAAIHDCLEEVEDSLDRVSRSCDEMKNLS 124

Query: 128 --RGTNFDLAMSNVQTWVSAALTDETTCSEGFQGKTVNGGVKGVVRTKIVNIAQLTSNAL 187
             +G +F   MSNV+TWVSAALTDETTC +GF GK ++G +K  VR ++V +A++TSNAL
Sbjct: 125 HAKGNDFTFRMSNVETWVSAALTDETTCMDGFAGKGMDGKIKESVRAQVVAVARVTSNAL 184

Query: 188 SLINQIGDLH 195
           +L+N     H
Sbjct: 185 ALVNNFAAKH 191

[Close]

BLAST of CSPI03G16000 vs. Swiss-Prot
Match: PME2_ARATH (Pectinesterase 2 OS=Arabidopsis thaliana GN=PME2 PE=2 SV=2)

HSP 1 Score: 75.9 bits (185), Expect = 5.6e-13
Identity = 52/169 (30.77%), Postives = 96/169 (56.80%), Query Frame = 1


Query: 26  ITPQSSTE--FIKSSCSSTTYPRLCFSSLSVHANAIQTSPRLLATAALSVSLSSVKSTAT 85
           IT  SST    +KS CSST YP LCFS+++       TS + +  A+L+++  +VK    
Sbjct: 51  ITTLSSTSHAILKSVCSSTLYPELCFSAVAATGGKELTSQKEVIEASLNLTTKAVKHNYF 110

Query: 86  QILKL-SHSHGLPSRDVSALDDCLEELSDSVDSLAASISEMPKL-RGTNFDLAMSNVQTW 145
            + KL +   GL  R+V+AL DCLE + +++D L  ++ ++ +  +  +      +++T 
Sbjct: 111 AVKKLIAKRKGLTPREVTALHDCLETIDETLDELHVAVEDLHQYPKQKSLRKHADDLKTL 170

Query: 146 VSAALTDETTCSEGFQGKTVNGGVKGVVRTKIVNIAQLTSNALSLINQI 191
           +S+A+T++ TC +GF     +  V+  +    V++  + SNAL++I  +
Sbjct: 171 ISSAITNQGTCLDGFSYDDADRKVRKALLKGQVHVEHMCSNALAMIKNM 219

[Close]

BLAST of CSPI03G16000 vs. Swiss-Prot
Match: PME1_CITSI (Pectinesterase 1 OS=Citrus sinensis GN=PECS-1.1 PE=2 SV=1)

HSP 1 Score: 74.3 bits (181), Expect = 1.6e-12
Identity = 49/161 (30.43%), Postives = 91/161 (56.52%), Query Frame = 1


Query: 35  IKSSCSSTTYPRLCFSSLSVHANAIQ--TSPRLLATAALSVSLSSVKSTATQILKLSHSH 94
           +KSSCSST YP LCFS+++    A +  TS + +   +L+++ ++V+     I KL    
Sbjct: 69  LKSSCSSTRYPDLCFSAIAAVPEASKKVTSQKDVIEMSLNITTTAVEHNYFGIQKLLKRT 128

Query: 95  GLPSRDVSALDDCLEELSDSVDSLAASISEMPKLRGT-NFDLAMSNVQTWVSAALTDETT 154
            L  R+  AL DCLE + +++D L  ++ ++ +     +      +++T +SAA+T++ T
Sbjct: 129 NLTKREKVALHDCLETIDETLDELHKAVEDLEEYPNKKSLSQHADDLKTLMSAAMTNQGT 188

Query: 155 CSEGFQGKTVNGGVKGVVRTKIVNIAQLTSNALSLINQIGD 193
           C +GF     N  V+  +    V++ ++ SNAL++I  + D
Sbjct: 189 CLDGFSHDDANKHVRDALSDGQVHVEKMCSNALAMIKNMTD 229

[Close]

BLAST of CSPI03G16000 vs. Swiss-Prot
Match: PME3_CITSI (Pectinesterase 3 OS=Citrus sinensis PE=1 SV=1)

HSP 1 Score: 74.3 bits (181), Expect = 1.6e-12
Identity = 49/161 (30.43%), Postives = 91/161 (56.52%), Query Frame = 1


Query: 35  IKSSCSSTTYPRLCFSSLSVHANAIQ--TSPRLLATAALSVSLSSVKSTATQILKLSHSH 94
           +KSSCSST YP LCFS+++    A +  TS + +   +L+++ ++V+     I KL    
Sbjct: 69  LKSSCSSTRYPDLCFSAIAAVPEASKKVTSQKDVIEMSLNITTTAVEHNYFGIQKLLKRT 128

Query: 95  GLPSRDVSALDDCLEELSDSVDSLAASISEMPKLRGT-NFDLAMSNVQTWVSAALTDETT 154
            L  R+  AL DCLE + +++D L  ++ ++ +     +      +++T +SAA+T++ T
Sbjct: 129 NLTKREKVALHDCLETIDETLDELHKAVEDLEEYPNKKSLSQHADDLKTLMSAAMTNQGT 188

Query: 155 CSEGFQGKTVNGGVKGVVRTKIVNIAQLTSNALSLINQIGD 193
           C +GF     N  V+  +    V++ ++ SNAL++I  + D
Sbjct: 189 CLDGFSHDDANKHVRDALSDGQVHVEKMCSNALAMIKNMTD 229

[Close]

BLAST of CSPI03G16000 vs. Swiss-Prot
Match: PME3_PHAVU (Pectinesterase 3 OS=Phaseolus vulgaris GN=MPE3 PE=2 SV=1)

HSP 1 Score: 71.2 bits (173), Expect = 1.4e-11
Identity = 55/167 (32.93%), Postives = 85/167 (50.90%), Query Frame = 1


Query: 27  TPQSSTEFIKSSCSSTTYPRLCFSSLSVHANAIQTSPRLLATAALSVSLSSVKSTATQIL 86
           T  S    +K+ C +T YP  CFSS+S    +  T P LL   +L V++  + S  +   
Sbjct: 69  TELSPAASLKAVCDTTRYPSSCFSSISSLPESNTTDPELLFKLSLRVAIDELSSFPS--- 128

Query: 87  KLSHSHGLPSRDVSALDDCLEELSDSVDSLAASISEMPKLRGTNFDLA-MSNVQTWVSAA 146
           KL  +    +R   A+D C     D++D L  SIS +  + G     A +SNV+TW+SAA
Sbjct: 129 KLRANAEQDARLQKAIDVCSSVFGDALDRLNDSISALGTVAGRIASSASVSNVETWLSAA 188

Query: 147 LTDETTCSE--GFQGKTVNGGVKGVVRTKIVNIAQLTSNALSLINQI 191
           LTD+ TC +  G    T   G    + T + N  +  SN+L+++ +I
Sbjct: 189 LTDQDTCLDAVGELNSTAARGALQEIETAMRNSTEFASNSLAIVTKI 232

[Close]

BLAST of CSPI03G16000 vs. TrEMBL
Match: A0A0A0L8D1_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_3G175740 PE=4 SV=1)

HSP 1 Score: 354.4 bits (908), Expect = 9.0e-95
Identity = 191/193 (98.96%), Postives = 192/193 (99.48%), Query Frame = 1


Query: 2   GISNSKKSLILFQLILQLTLIHSAITPQSSTEFIKSSCSSTTYPRLCFSSLSVHANAIQT 61
           GISNSKKSLILFQLILQLTLIHSAITPQSSTEFIKSSCSSTTYPRLCFSSLSVHANAIQT
Sbjct: 44  GISNSKKSLILFQLILQLTLIHSAITPQSSTEFIKSSCSSTTYPRLCFSSLSVHANAIQT 103

Query: 62  SPRLLATAALSVSLSSVKSTATQILKLSHSHGLPSRDVSALDDCLEELSDSVDSLAASIS 121
           SPRLLATAALSVSLSSVKSTATQILKLSHSHGLPSRDVSALDDCLEELSDSVDSLAASIS
Sbjct: 104 SPRLLATAALSVSLSSVKSTATQILKLSHSHGLPSRDVSALDDCLEELSDSVDSLAASIS 163

Query: 122 EMPKLRGTNFDLAMSNVQTWVSAALTDETTCSEGFQGKTVNGGVKGVVRTKIVNIAQLTS 181
           EMPKLRGTNFDLAMSNVQTWVSAALTDETTCSEGFQGKTVNGGVKG VRTKIVNIAQLTS
Sbjct: 164 EMPKLRGTNFDLAMSNVQTWVSAALTDETTCSEGFQGKTVNGGVKGAVRTKIVNIAQLTS 223

Query: 182 NALSLINQIGDLH 195
           NALSLIN+IGDLH
Sbjct: 224 NALSLINRIGDLH 236

[Close]

BLAST of CSPI03G16000 vs. TrEMBL
Match: A0A0A0LB27_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_3G176245 PE=4 SV=1)

HSP 1 Score: 352.4 bits (903), Expect = 3.4e-94
Identity = 190/194 (97.94%), Postives = 192/194 (98.97%), Query Frame = 1


Query: 1   MGISNSKKSLILFQLILQLTLIHSAITPQSSTEFIKSSCSSTTYPRLCFSSLSVHANAIQ 60
           MGISNSKK+LILFQLILQLTLIHSAITPQSSTEFIKSSCSSTTYPRLCFSSLSVHANAIQ
Sbjct: 1   MGISNSKKTLILFQLILQLTLIHSAITPQSSTEFIKSSCSSTTYPRLCFSSLSVHANAIQ 60

Query: 61  TSPRLLATAALSVSLSSVKSTATQILKLSHSHGLPSRDVSALDDCLEELSDSVDSLAASI 120
           TSPRLLATAALSVSLSSVKSTATQILKLSHSHGLPSRDVSALDDCLEELSDSVDSLAASI
Sbjct: 61  TSPRLLATAALSVSLSSVKSTATQILKLSHSHGLPSRDVSALDDCLEELSDSVDSLAASI 120

Query: 121 SEMPKLRGTNFDLAMSNVQTWVSAALTDETTCSEGFQGKTVNGGVKGVVRTKIVNIAQLT 180
           SEMPKLRGTNFDLAMSNVQTWVSAALTDETTCSEGFQGKTVNGGVKG VRTKIVNIAQLT
Sbjct: 121 SEMPKLRGTNFDLAMSNVQTWVSAALTDETTCSEGFQGKTVNGGVKGAVRTKIVNIAQLT 180

Query: 181 SNALSLINQIGDLH 195
           SNALSLIN+I DLH
Sbjct: 181 SNALSLINRIADLH 194

[Close]

BLAST of CSPI03G16000 vs. TrEMBL
Match: A0A061G793_THECC (DC1.2-like, putative OS=Theobroma cacao GN=TCM_014946 PE=4 SV=1)

HSP 1 Score: 209.1 bits (531), Expect = 4.7e-51
Identity = 114/194 (58.76%), Postives = 146/194 (75.26%), Query Frame = 1


Query: 4   SNSKKSLILFQLILQLTLIHSA---ITPQSSTEFIKSSCSSTTYPRLCFSSLSVHANAIQ 63
           S+S K L++   I       SA   I PQSS EFI++SCSSTTYP+LCF +LS HA+ IQ
Sbjct: 7   SHSFKFLLILLAINSYINFSSAGRQIAPQSSAEFIRTSCSSTTYPKLCFETLSTHASLIQ 66

Query: 64  TSPRLLATAALSVSLSSVKSTATQILKLSHSHGLPSRDVSALDDCLEELSDSVDSLAASI 123
           TSP+LLA AAL+V+LS+ +ST+  ++ LS SHGL  R+  A+ DC+EELSDS+D L  SI
Sbjct: 67  TSPQLLAHAALNVTLSTTESTSAMMVTLSKSHGLKPREAEAMQDCVEELSDSIDELRKSI 126

Query: 124 SEMPKLRGTNFDLAMSNVQTWVSAALTDETTCSEGFQGKTVNGGVKGVVRTKIVNIAQLT 183
           SEM +++G+NF L +++VQTWVSAALTDE+TCS+GFQG  +NG VK  VRTKI+ IA LT
Sbjct: 127 SEMGQVKGSNFGLMINDVQTWVSAALTDESTCSDGFQGNNMNGNVKTAVRTKILRIAHLT 186

Query: 184 SNALSLINQIGDLH 195
           SNAL+LIN    LH
Sbjct: 187 SNALALINNYASLH 200

[Close]

BLAST of CSPI03G16000 vs. TrEMBL
Match: M5XKR0_PRUPE (Uncharacterized protein OS=Prunus persica GN=PRUPE_ppa011607mg PE=4 SV=1)

HSP 1 Score: 203.4 bits (516), Expect = 2.6e-49
Identity = 108/179 (60.34%), Postives = 139/179 (77.65%), Query Frame = 1


Query: 16  ILQLTLIHSAITPQSSTEFIKSSCSSTTYPRLCFSSLSVHANAIQTSPRLLATAALSVSL 75
           I+  T     + P+++TEFIK+SCS+TTYP+LC +SLS HA+ IQTSP+L+A AAL+V+L
Sbjct: 24  IISSTSAARDLAPKTNTEFIKTSCSATTYPKLCLTSLSSHASTIQTSPKLMAGAALNVTL 83

Query: 76  SSVKSTATQILKLSHSHGLPSRDVSALDDCLEELSDSVDSLAASISEMPKLRGTNFDLAM 135
           SS KST+  +LKLS SHGL  ++V A+ DCLEELSDSVD L +SI+EM   +  +F L +
Sbjct: 84  SSAKSTSAVMLKLSQSHGLKPKEVGAMRDCLEELSDSVDELQSSIAEMGNFKSYDFQLMI 143

Query: 136 SNVQTWVSAALTDETTCSEGFQGKTVNGGVKGVVRTKIVNIAQLTSNALSLINQIGDLH 195
           S+VQTWVSAALTDE TCSEGF G  +NG +K  VR +IVNIAQLTSNAL+LIN+   +H
Sbjct: 144 SDVQTWVSAALTDENTCSEGFGGNGMNGNLKTAVRGRIVNIAQLTSNALALINRYASVH 202

[Close]

BLAST of CSPI03G16000 vs. TrEMBL
Match: B9R9R0_RICCO (21 kDa protein, putative OS=Ricinus communis GN=RCOM_1500140 PE=4 SV=1)

HSP 1 Score: 199.5 bits (506), Expect = 3.7e-48
Identity = 106/195 (54.36%), Postives = 145/195 (74.36%), Query Frame = 1


Query: 4   SNSKKSLILFQLILQLTLIHSA----ITPQSSTEFIKSSCSSTTYPRLCFSSLSVHANAI 63
           SN+  + +L  L +   +  S+    +   +STEFI++SCS+TTYPRLC+SSL +HA+ I
Sbjct: 6   SNTFSNFLLILLAISFHINSSSAGRNLAQTTSTEFIRTSCSTTTYPRLCYSSLKIHASKI 65

Query: 64  QTSPRLLATAALSVSLSSVKSTATQILKLSHSHGLPSRDVSALDDCLEELSDSVDSLAAS 123
           QTSP LLA AAL+V+L+S +ST+T + KLS SHG+  R+VSA+ DC++EL+DSVD L  S
Sbjct: 66  QTSPMLLANAALNVTLASTRSTSTMMQKLSKSHGMKPREVSAMQDCMDELTDSVDELRKS 125

Query: 124 ISEMPKLRGTNFDLAMSNVQTWVSAALTDETTCSEGFQGKTVNGGVKGVVRTKIVNIAQL 183
           I E+ K +G+ F L M++VQTWVSAALTDE+TCS+GF G T+NG +K +VR + V IA L
Sbjct: 126 IDELGKAQGSKFGLMMNDVQTWVSAALTDESTCSDGFAGSTMNGELKTLVRQQTVKIAHL 185

Query: 184 TSNALSLINQIGDLH 195
           TSNALSL+N    +H
Sbjct: 186 TSNALSLVNSYASVH 200

[Close]

BLAST of CSPI03G16000 vs. TAIR10
Match: AT5G62360.1 (AT5G62360.1 Plant invertase/pectin methylesterase inhibitor superfamily protein)

HSP 1 Score: 178.3 bits (451), Expect = 4.5e-45
Identity = 95/184 (51.63%), Postives = 140/184 (76.09%), Query Frame = 1


Query: 15  LILQLTLIHSAITPQSST----EFIKSSCSSTTYPRLCFSSLSVHANAIQTSPRLLATAA 74
           +I  L L+H+  T  ++T    EF+KSSC+ TTYPRLCFSSLS HA+ IQTSP+L+A AA
Sbjct: 20  IIAMLKLVHTTTTTTTTTTTNTEFVKSSCTFTTYPRLCFSSLSTHASLIQTSPKLMAHAA 79

Query: 75  LSVSLSSVKSTATQILKLSHSHGLPSRDVSALDDCLEELSDSVDSLAASISEMPKLRGTN 134
           L+++L+S K T+  +++LS+S  L  ++VSA+ DC+EEL D+++ L  SI EM +L G+N
Sbjct: 80  LNITLASAKVTSAMMVRLSNSR-LKPKEVSAMRDCVEELGDTLEELRKSIGEMCQLSGSN 139

Query: 135 FDLAMSNVQTWVSAALTDETTCSEGFQGKTVNGGVKGVVRTKIVNIAQLTSNALSLINQI 194
           +++ +S++QTWVSAALTD  TC++GF+G+ ++G VK +VR +I+ IA LTSNAL+LIN  
Sbjct: 140 YEVYISDIQTWVSAALTDVNTCTDGFEGEDMDGKVKVLVRGRILVIAHLTSNALALINHF 199

[Close]

BLAST of CSPI03G16000 vs. TAIR10
Match: AT5G62350.1 (AT5G62350.1 Plant invertase/pectin methylesterase inhibitor superfamily protein)

HSP 1 Score: 151.4 bits (381), Expect = 5.9e-37
Identity = 91/186 (48.92%), Postives = 124/186 (66.67%), Query Frame = 1


Query: 11  ILFQLILQLTLIHSAITPQSSTE---FIKSSCSSTTYPRLCFSSLSVHANAIQTSPRLLA 70
           I + L L+LT   +A    +S +   FI+SSC +TTYP LC  SLSV+AN IQTSP+ LA
Sbjct: 12  ISYLLSLELTAATAASQTGASKKAINFIQSSCKTTTYPALCVHSLSVYANDIQTSPKRLA 71

Query: 71  TAALSVSLSSVKSTATQILKLSHSHGLPSRDVSALDDCLEELSDSVDSLAASISEMPKLR 130
             A++V+LS  +ST   + +L+   GL  R+V A+ DC+EE++D+VD L  S+ E+ KL 
Sbjct: 72  ETAIAVTLSRAQSTKLFVSRLTRMKGLKKREVEAIKDCVEEMNDTVDRLTKSVQEL-KLC 131

Query: 131 GT-----NFDLAMSNVQTWVSAALTDETTCSEGFQGKTVNGGVKGVVRTKIVNIAQLTSN 189
           G+      F   MSN QTW SAALTDE TCS+GF G+ ++G +K  VR +I+N+   TSN
Sbjct: 132 GSAKDQDQFAYHMSNAQTWTSAALTDENTCSDGFSGRVMDGRIKNSVRARIMNVGHETSN 191

[Close]

BLAST of CSPI03G16000 vs. TAIR10
Match: AT4G25260.1 (AT4G25260.1 Plant invertase/pectin methylesterase inhibitor superfamily protein)

HSP 1 Score: 150.2 bits (378), Expect = 1.3e-36
Identity = 91/198 (45.96%), Postives = 126/198 (63.64%), Query Frame = 1


Query: 5   NSKKSLILFQLILQLTLIHSA--ITPQSS--TEFIKSSCSSTTYPRLCFSSLSVHANAIQ 64
           N + SLILF L L    I  A  +  +SS  TEFIK+SC +T+YP  CF SLS +A+ I+
Sbjct: 4   NFELSLILFVLYLSTAAIVMARNLEEESSGDTEFIKASCETTSYPDRCFQSLSSYASEIK 63

Query: 65  TSPRLLATAALSVSLSSVKSTATQILKLSHSHGLPSRDVSALDDCLEELSDSVDSLAASI 124
             PR LA  AL+VS++  KS  T + +++   G+  R   A+ DCLEE+ D+VD L+ S+
Sbjct: 64  KQPRKLAETALAVSIARAKSAKTYVSEMTDYKGITKRQHEAVADCLEEMGDTVDRLSNSL 123

Query: 125 SEMPKLR----GTNFDLAMSNVQTWVSAALTDETTCSEGFQGKTVNGGVKGVVRTKIVNI 184
            E+  L     G +F   +SNV+TW SAALTDET C +GF GK + G +K ++RT IV++
Sbjct: 124 KELKHLEEGDSGEDFWFCLSNVRTWTSAALTDETACMDGFGGKAMAGELKSLIRTHIVSV 183

Query: 185 AQLTSNALSLINQIGDLH 195
           A+ TSNAL+LIN     H
Sbjct: 184 AEETSNALALINDFASKH 201

[Close]

BLAST of CSPI03G16000 vs. TAIR10
Match: AT3G47380.1 (AT3G47380.1 Plant invertase/pectin methylesterase inhibitor superfamily protein)

HSP 1 Score: 140.2 bits (352), Expect = 1.4e-33
Identity = 79/170 (46.47%), Postives = 116/170 (68.24%), Query Frame = 1


Query: 24  SAITPQSSTEFIKSSCSSTTYPRLCFSSLSVHANAIQTSPRLLATAALSVSLSSVKSTAT 83
           +AIT + +  FI++SC +TTYP +C +SL+ +AN+IQTSPR LA  AL+V+++  +ST  
Sbjct: 28  AAITSKRAINFIQASCKATTYPTVCVNSLTGYANSIQTSPRRLAETALNVTVTQAQSTKV 87

Query: 84  QILKLSHSHGLPSRDVSALDDCLEELSDSVDSLAASISEMPKLRGT-----NFDLAMSNV 143
            + +L     L  R++ A+ DC+EE+ D+VD L  SI E+ K+ G+      F   MSN 
Sbjct: 88  FVWRLGRFTSLKKREIQAVKDCIEEIHDAVDRLTMSIHEV-KMCGSAKGRDQFWFHMSNA 147

Query: 144 QTWVSAALTDETTCSEGFQGKTVNGGVKGVVRTKIVNIAQLTSNALSLIN 189
           QTW SAALT+  TCS+GF G+ ++G VK  VR +I+N+ + TSNAL+LIN
Sbjct: 148 QTWTSAALTNANTCSDGFAGRVMDGRVKNSVRARILNLGRGTSNALALIN 196

[Close]

BLAST of CSPI03G16000 vs. TAIR10
Match: AT1G62760.1 (AT1G62760.1 Plant invertase/pectin methylesterase inhibitor superfamily protein)

HSP 1 Score: 131.0 bits (328), Expect = 8.2e-31
Identity = 74/168 (44.05%), Postives = 114/168 (67.86%), Query Frame = 1


Query: 24  SAITPQSSTEFIKSSCSSTTYPRLCFSSLSVHANAIQTSPRLLATAALSVSLSSVKSTAT 83
           S  + Q++ ++IK+SC+ T Y  +C++SLS +A+ I+++P+ LA  AL+++LSS KS + 
Sbjct: 137 STYSNQTNLDYIKTSCNITLYKTICYNSLSPYASTIRSNPQKLAVIALNLTLSSAKSASK 196

Query: 84  QILKLSHSHGLPSRDVSALDDCLEELSDSVDSLAASISEMPKLR---GTNFDLAMSNVQT 143
            +  +SH  GL   +V A+ DC+EE+ DSV SL  SI E+  +       F++ MS+V+T
Sbjct: 197 FVKNISHGGGLTRLEVVAVADCVEEIGDSVTSLQDSIRELDSINYKDSAKFEMVMSDVET 256

Query: 144 WVSAALTDETTCSEGFQGKTVNGGVKGVVRTKIVNIAQLTSNALSLIN 189
           WVSAALT++ TC +GF    V   VK +VR  +V +A+LTSNAL+LIN
Sbjct: 257 WVSAALTNDDTCMDGF--SLVKTAVKDLVRRHVVEVARLTSNALALIN 302

[Close]

BLAST of CSPI03G16000 vs. NCBI nr
Match: gi|778688522|ref|XP_011652768.1| (PREDICTED: 21 kDa protein-like [Cucumis sativus])

HSP 1 Score: 354.4 bits (908), Expect = 1.3e-94
Identity = 191/193 (98.96%), Postives = 192/193 (99.48%), Query Frame = 1


Query: 2   GISNSKKSLILFQLILQLTLIHSAITPQSSTEFIKSSCSSTTYPRLCFSSLSVHANAIQT 61
           GISNSKKSLILFQLILQLTLIHSAITPQSSTEFIKSSCSSTTYPRLCFSSLSVHANAIQT
Sbjct: 44  GISNSKKSLILFQLILQLTLIHSAITPQSSTEFIKSSCSSTTYPRLCFSSLSVHANAIQT 103

Query: 62  SPRLLATAALSVSLSSVKSTATQILKLSHSHGLPSRDVSALDDCLEELSDSVDSLAASIS 121
           SPRLLATAALSVSLSSVKSTATQILKLSHSHGLPSRDVSALDDCLEELSDSVDSLAASIS
Sbjct: 104 SPRLLATAALSVSLSSVKSTATQILKLSHSHGLPSRDVSALDDCLEELSDSVDSLAASIS 163

Query: 122 EMPKLRGTNFDLAMSNVQTWVSAALTDETTCSEGFQGKTVNGGVKGVVRTKIVNIAQLTS 181
           EMPKLRGTNFDLAMSNVQTWVSAALTDETTCSEGFQGKTVNGGVKG VRTKIVNIAQLTS
Sbjct: 164 EMPKLRGTNFDLAMSNVQTWVSAALTDETTCSEGFQGKTVNGGVKGAVRTKIVNIAQLTS 223

Query: 182 NALSLINQIGDLH 195
           NALSLIN+IGDLH
Sbjct: 224 NALSLINRIGDLH 236

[Close]

BLAST of CSPI03G16000 vs. NCBI nr
Match: gi|778679325|ref|XP_004148138.2| (PREDICTED: 21 kDa protein [Cucumis sativus])

HSP 1 Score: 352.4 bits (903), Expect = 4.9e-94
Identity = 190/194 (97.94%), Postives = 192/194 (98.97%), Query Frame = 1


Query: 1   MGISNSKKSLILFQLILQLTLIHSAITPQSSTEFIKSSCSSTTYPRLCFSSLSVHANAIQ 60
           MGISNSKK+LILFQLILQLTLIHSAITPQSSTEFIKSSCSSTTYPRLCFSSLSVHANAIQ
Sbjct: 1   MGISNSKKTLILFQLILQLTLIHSAITPQSSTEFIKSSCSSTTYPRLCFSSLSVHANAIQ 60

Query: 61  TSPRLLATAALSVSLSSVKSTATQILKLSHSHGLPSRDVSALDDCLEELSDSVDSLAASI 120
           TSPRLLATAALSVSLSSVKSTATQILKLSHSHGLPSRDVSALDDCLEELSDSVDSLAASI
Sbjct: 61  TSPRLLATAALSVSLSSVKSTATQILKLSHSHGLPSRDVSALDDCLEELSDSVDSLAASI 120

Query: 121 SEMPKLRGTNFDLAMSNVQTWVSAALTDETTCSEGFQGKTVNGGVKGVVRTKIVNIAQLT 180
           SEMPKLRGTNFDLAMSNVQTWVSAALTDETTCSEGFQGKTVNGGVKG VRTKIVNIAQLT
Sbjct: 121 SEMPKLRGTNFDLAMSNVQTWVSAALTDETTCSEGFQGKTVNGGVKGAVRTKIVNIAQLT 180

Query: 181 SNALSLINQIGDLH 195
           SNALSLIN+I DLH
Sbjct: 181 SNALSLINRIADLH 194

[Close]

BLAST of CSPI03G16000 vs. NCBI nr
Match: gi|659133745|ref|XP_008466883.1| (PREDICTED: 21 kDa protein-like, partial [Cucumis melo])

HSP 1 Score: 209.5 bits (532), Expect = 5.2e-51
Identity = 117/122 (95.90%), Postives = 119/122 (97.54%), Query Frame = 1


Query: 1   MGISNSKKSLILFQLILQLTLIHSAITPQSSTEFIKSSCSSTTYPRLCFSSLSVHANAIQ 60
           MGISN KKSLILFQLILQLT+IHSAITPQSSTEFIKSSCSSTTYPRLCFSSLSVHANAIQ
Sbjct: 1   MGISNCKKSLILFQLILQLTIIHSAITPQSSTEFIKSSCSSTTYPRLCFSSLSVHANAIQ 60

Query: 61  TSPRLLATAALSVSLSSVKSTATQILKLSHSHGLPSRDVSALDDCLEELSDSVDSLAASI 120
           TSPRLLATAALSVSLSSVKST TQILKLSHSHGL SRDVSAL+DCLEELSDSVDSLAASI
Sbjct: 61  TSPRLLATAALSVSLSSVKSTTTQILKLSHSHGLSSRDVSALNDCLEELSDSVDSLAASI 120

Query: 121 SE 123
           SE
Sbjct: 121 SE 122

[Close]

BLAST of CSPI03G16000 vs. NCBI nr
Match: gi|590671750|ref|XP_007038418.1| (DC1.2-like, putative [Theobroma cacao])

HSP 1 Score: 209.1 bits (531), Expect = 6.7e-51
Identity = 114/194 (58.76%), Postives = 146/194 (75.26%), Query Frame = 1


Query: 4   SNSKKSLILFQLILQLTLIHSA---ITPQSSTEFIKSSCSSTTYPRLCFSSLSVHANAIQ 63
           S+S K L++   I       SA   I PQSS EFI++SCSSTTYP+LCF +LS HA+ IQ
Sbjct: 7   SHSFKFLLILLAINSYINFSSAGRQIAPQSSAEFIRTSCSSTTYPKLCFETLSTHASLIQ 66

Query: 64  TSPRLLATAALSVSLSSVKSTATQILKLSHSHGLPSRDVSALDDCLEELSDSVDSLAASI 123
           TSP+LLA AAL+V+LS+ +ST+  ++ LS SHGL  R+  A+ DC+EELSDS+D L  SI
Sbjct: 67  TSPQLLAHAALNVTLSTTESTSAMMVTLSKSHGLKPREAEAMQDCVEELSDSIDELRKSI 126

Query: 124 SEMPKLRGTNFDLAMSNVQTWVSAALTDETTCSEGFQGKTVNGGVKGVVRTKIVNIAQLT 183
           SEM +++G+NF L +++VQTWVSAALTDE+TCS+GFQG  +NG VK  VRTKI+ IA LT
Sbjct: 127 SEMGQVKGSNFGLMINDVQTWVSAALTDESTCSDGFQGNNMNGNVKTAVRTKILRIAHLT 186

Query: 184 SNALSLINQIGDLH 195
           SNAL+LIN    LH
Sbjct: 187 SNALALINNYASLH 200

[Close]

BLAST of CSPI03G16000 vs. NCBI nr
Match: gi|596006342|ref|XP_007218426.1| (hypothetical protein PRUPE_ppa011607mg [Prunus persica])

HSP 1 Score: 203.4 bits (516), Expect = 3.7e-49
Identity = 108/179 (60.34%), Postives = 139/179 (77.65%), Query Frame = 1


Query: 16  ILQLTLIHSAITPQSSTEFIKSSCSSTTYPRLCFSSLSVHANAIQTSPRLLATAALSVSL 75
           I+  T     + P+++TEFIK+SCS+TTYP+LC +SLS HA+ IQTSP+L+A AAL+V+L
Sbjct: 24  IISSTSAARDLAPKTNTEFIKTSCSATTYPKLCLTSLSSHASTIQTSPKLMAGAALNVTL 83

Query: 76  SSVKSTATQILKLSHSHGLPSRDVSALDDCLEELSDSVDSLAASISEMPKLRGTNFDLAM 135
           SS KST+  +LKLS SHGL  ++V A+ DCLEELSDSVD L +SI+EM   +  +F L +
Sbjct: 84  SSAKSTSAVMLKLSQSHGLKPKEVGAMRDCLEELSDSVDELQSSIAEMGNFKSYDFQLMI 143

Query: 136 SNVQTWVSAALTDETTCSEGFQGKTVNGGVKGVVRTKIVNIAQLTSNALSLINQIGDLH 195
           S+VQTWVSAALTDE TCSEGF G  +NG +K  VR +IVNIAQLTSNAL+LIN+   +H
Sbjct: 144 SDVQTWVSAALTDENTCSEGFGGNGMNGNLKTAVRGRIVNIAQLTSNALALINRYASVH 202

The following BLAST results are available for this feature:

BLAST of CSPI03G16000 vs. Swiss-Prot


Match Name	E-value	Identity	Description
21KD_DAUCA	1.8e-35	44.74	21 kDa protein OS=Daucus carota PE=2 SV=1	[more]
PME2_ARATH	5.6e-13	30.77	Pectinesterase 2 OS=Arabidopsis thaliana GN=PME2 PE=2 SV=2	[more]
PME1_CITSI	1.6e-12	30.43	Pectinesterase 1 OS=Citrus sinensis GN=PECS-1.1 PE=2 SV=1	[more]
PME3_CITSI	1.6e-12	30.43	Pectinesterase 3 OS=Citrus sinensis PE=1 SV=1	[more]
PME3_PHAVU	1.4e-11	32.93	Pectinesterase 3 OS=Phaseolus vulgaris GN=MPE3 PE=2 SV=1	[more]

BLAST of CSPI03G16000 vs. TrEMBL


Match Name	E-value	Identity	Description
A0A0A0L8D1_CUCSA	9.0e-95	98.96	Uncharacterized protein OS=Cucumis sativus GN=Csa_3G175740 PE=4 SV=1	[more]
A0A0A0LB27_CUCSA	3.4e-94	97.94	Uncharacterized protein OS=Cucumis sativus GN=Csa_3G176245 PE=4 SV=1	[more]
A0A061G793_THECC	4.7e-51	58.76	DC1.2-like, putative OS=Theobroma cacao GN=TCM_014946 PE=4 SV=1	[more]
M5XKR0_PRUPE	2.6e-49	60.34	Uncharacterized protein OS=Prunus persica GN=PRUPE_ppa011607mg PE=4 SV=1	[more]
B9R9R0_RICCO	3.7e-48	54.36	21 kDa protein, putative OS=Ricinus communis GN=RCOM_1500140 PE=4 SV=1	[more]

BLAST of CSPI03G16000 vs. TAIR10


Match Name	E-value	Identity	Description
AT5G62360.1	4.5e-45	51.63	Plant invertase/pectin methylesterase inhibitor superfamily protein	[more]
AT5G62350.1	5.9e-37	48.92	Plant invertase/pectin methylesterase inhibitor superfamily protein	[more]
AT4G25260.1	1.3e-36	45.96	Plant invertase/pectin methylesterase inhibitor superfamily protein	[more]
AT3G47380.1	1.4e-33	46.47	Plant invertase/pectin methylesterase inhibitor superfamily protein	[more]
AT1G62760.1	8.2e-31	44.05	Plant invertase/pectin methylesterase inhibitor superfamily protein	[more]

BLAST of CSPI03G16000 vs. NCBI nr


Match Name	E-value	Identity	Description
gi\|778688522\|ref\|XP_011652768.1\|	1.3e-94	98.96	PREDICTED: 21 kDa protein-like [Cucumis sativus]	[more]
gi\|778679325\|ref\|XP_004148138.2\|	4.9e-94	97.94	PREDICTED: 21 kDa protein [Cucumis sativus]	[more]
gi\|659133745\|ref\|XP_008466883.1\|	5.2e-51	95.90	PREDICTED: 21 kDa protein-like, partial [Cucumis melo]	[more]
gi\|590671750\|ref\|XP_007038418.1\|	6.7e-51	58.76	DC1.2-like, putative [Theobroma cacao]	[more]
gi\|596006342\|ref\|XP_007218426.1\|	3.7e-49	60.34	hypothetical protein PRUPE_ppa011607mg [Prunus persica]	[more]

Annotated Terms

The following terms have been associated with this gene:

Vocabulary: **INTERPRO**
Term	Definition
IPR006501	Pectinesterase_inhib_dom

Vocabulary: **Molecular Function**
Term	Definition
GO:0004857	enzyme inhibitor activity

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0043086	negative regulation of catalytic activity
biological_process	GO:0005982	starch metabolic process
biological_process	GO:0005985	sucrose metabolic process
biological_process	GO:0008150	biological_process
cellular_component	GO:0005575	cellular_component
molecular_function	GO:0004857	enzyme inhibitor activity
molecular_function	GO:0030599	pectinesterase activity

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CSPI03G16000.1	CSPI03G16000.1	mRNA

InterPro

Analysis Name: InterPro Annotations of cucumber (PI183967)
Date Performed: 2017-01-17


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR006501	Pectinesterase inhibitor domain	GENE3D	G3DSA:1.20.140.40		coord: 31..190 score: 8.9
IPR006501	Pectinesterase inhibitor domain	PFAM	PF04043	PMEI	coord: 34..186 score: 2.2
IPR006501	Pectinesterase inhibitor domain	SMART	SM00856	PMEI_2	coord: 30..186 score: 3.4
IPR006501	Pectinesterase inhibitor domain	TIGRFAMs	TIGR01614	TIGR01614	coord: 7..190 score: 1.7
IPR006501	Pectinesterase inhibitor domain	unknown	SSF101148	Plant invertase/pectin methylesterase inhibitor	coord: 33..190 score: 8.89
None	No IPR available	PANTHER	PTHR31080	FAMILY NOT NAMED	coord: 1..194 score: 2.0
None	No IPR available	PANTHER	PTHR31080:SF19	PLANT INVERTASE/PECTIN METHYLESTERASE INHIBITOR SUPERFAMILY PROTEIN	coord: 1..194 score: 2.0

Synteny

The following gene(s) are orthologous to this gene:


Gene	Orthologue	Organism	Block
CSPI03G16000	Cla011550	Watermelon (97103) v1	cpiwmB261
CSPI03G16000	Cla006266	Watermelon (97103) v1	cpiwmB231
CSPI03G16000	Csa3G175740	Cucumber (Chinese Long) v2	cpicuB120
CSPI03G16000	Csa5G160140	Cucumber (Chinese Long) v2	cpicuB141
CSPI03G16000	MELO3C005566	Melon (DHL92) v3.5.1	cpimeB163
CSPI03G16000	MELO3C006819	Melon (DHL92) v3.5.1	cpimeB230
CSPI03G16000	ClCG01G003740	Watermelon (Charleston Gray)	cpiwcgB210
CSPI03G16000	Lsi05G013170	Bottle gourd (USVL1VR-Ls)	cpilsiB208
CSPI03G16000	Lsi09G003880	Bottle gourd (USVL1VR-Ls)	cpilsiB155
CSPI03G16000	MELO3C005567.2	Melon (DHL92) v3.6.1	cpimedB154
CSPI03G16000	MELO3C006819.2	Melon (DHL92) v3.6.1	cpimedB224
CSPI03G16000	CsaV3_3G016210	Cucumber (Chinese Long) v3	cpicucB144
CSPI03G16000	CsaV3_5G003880	Cucumber (Chinese Long) v3	cpicucB169
CSPI03G16000	Cla97C01G003880	Watermelon (97103) v2	cpiwmbB181
CSPI03G16000	Cla97C05G089170	Watermelon (97103) v2	cpiwmbB209
CSPI03G16000	Bhi01G000900	Wax gourd	cpiwgoB269
CSPI03G16000	Cucsa.302150	Cucumber (Gy14) v1	cgycpiB453
CSPI03G16000	CmaCh15G011540	Cucurbita maxima (Rimu)	cmacpiB286
CSPI03G16000	CmoCh04G003470	Cucurbita moschata (Rifu)	cmocpiB704
CSPI03G16000	CmoCh15G012160	Cucurbita moschata (Rifu)	cmocpiB276
CSPI03G16000	Cp4.1LG13g02110	Cucurbita pepo (Zucchini)	cpecpiB171
CSPI03G16000	CsGy3G016070	Cucumber (Gy14) v2	cgybcpiB110
CSPI03G16000	CsGy5G003770	Cucumber (Gy14) v2	cgybcpiB213
CSPI03G16000	Carg02463	Silver-seed gourd	carcpiB0955

The following gene(s) are paralogous to this gene:


Gene	Paralogue	Organism	Block
CSPI03G16000	CSPI05G06470	Wild cucumber (PI 183967)	cpicpiB116

The following block(s) are covering this gene:


Gene	Organism	Block
CSPI03G16000	Cucurbita maxima (Rimu)	cmacpiB335
CSPI03G16000	Cucurbita maxima (Rimu)	cmacpiB601
CSPI03G16000	Cucurbita maxima (Rimu)	cmacpiB609
CSPI03G16000	Cucurbita maxima (Rimu)	cmacpiB712
CSPI03G16000	Cucurbita moschata (Rifu)	cmocpiB325
CSPI03G16000	Cucurbita moschata (Rifu)	cmocpiB589
CSPI03G16000	Cucurbita moschata (Rifu)	cmocpiB598
CSPI03G16000	Watermelon (Charleston Gray)	cpiwcgB226
CSPI03G16000	Cucurbita pepo (Zucchini)	cpecpiB217
CSPI03G16000	Cucurbita pepo (Zucchini)	cpecpiB393
CSPI03G16000	Cucurbita pepo (Zucchini)	cpecpiB721
CSPI03G16000	Cucurbita pepo (Zucchini)	cpecpiB725
CSPI03G16000	Silver-seed gourd	carcpiB0406
CSPI03G16000	Silver-seed gourd	carcpiB0905
CSPI03G16000	Cucumber (Gy14) v1	cgycpiB383